biopipen 0.21.0__py3-none-any.whl → 0.34.26__py3-none-any.whl

biopipen/scripts/scrna_metabolic_landscape/MetabolicFeatures.R

@@ -1,112 +1,477 @@
-source("{{biopipen_dir}}/utils/gsea.R")
-
-library(parallel)
+library(rlang)
 library(Seurat)
+library(biopipen.utils)
+library(enrichit)
+library(tidyseurat)
 
 sobjfile <- {{ in.sobjfile | r }}
 outdir <- {{ out.outdir | r }}
-gmtfile <- {{ envs.gmtfile | r }}
 ncores <- {{ envs.ncores | r }}
-fgsea <- {{ envs.fgsea | r }}
-top <- {{ envs.top | r }}
 prerank_method <- {{ envs.prerank_method | r }}
-grouping <- {{ envs.grouping | r }}
-grouping_prefix <- {{ envs.grouping_prefix | r }}
-subsetting_cols <- {{ envs.subsetting | r }}
-subsetting_prefix <- {{ envs.subsetting_prefix | r }}
+gmtfile <- {{ envs.gmtfile | r }}
+subset_by <- {{ envs.subset_by | r }}
+group_by <- {{ envs.group_by | r }}
+comparisons <- {{ envs.comparisons | r }}
+fgsea_args <- {{ envs.fgsea_args | r }}
+plots <- {{ envs.plots | r }}
+cases <- {{ envs.cases | r }}
 
-if (!is.null(grouping_prefix) && nchar(grouping_prefix) > 0) {
-    grouping_prefix = paste0(grouping_prefix, "_")
-}
+set.seed(8525)
 
-if (!is.null(subsetting_prefix) && nchar(subsetting_prefix) > 0) {
-    subsetting_prefix = paste0(subsetting_prefix, "_")
-}
+log <- get_logger()
+reporter <- get_reporter()
 
-set.seed(8525)
+log$info("Loading Seurat object ...")
+sobj <- read_obj(sobjfile)
 
-## gmt_pathways is copied from fgsea package.
-gmt_pathways <- function(gmt_file) {
-    pathway_lines <- strsplit(readLines(gmt_file), "\t")
-    pathways <- lapply(pathway_lines, tail, -2)
-    names(pathways) <- sapply(pathway_lines, head, 1)
-    pathways
-}
+defaults <- list(
+    prerank_method = prerank_method,
+    subset_by = subset_by,
+    group_by = group_by,
+    comparisons = comparisons,
+    fgsea_args = fgsea_args,
+    plots = plots
+)
+log$info("Expanding cases ...")
+default_case <- subset_by %||% "DEFAULT"
+cases <- expand_cases(
+    cases,
+    defaults,
+    function(name, case) {
+        if (is.null(case$group_by)) {
+            stop("'group_by' is required in case: ", name)
+        }
+        stats::setNames(list(case), name)
+    },
+    default_case = default_case)
 
-pathways <- gmt_pathways(gmtfile)
+log$info("Loading metabolic pathways ...")
+pathways <- ParseGMT(gmtfile)
+pathway_names <- names(pathways)
 metabolics <- unique(as.vector(unname(unlist(pathways))))
-sobj <- readRDS(sobjfile)
-
-do_one_group <- function(obj, group, outputdir) {
-    print(paste("- Processing group", grouping, ":", group))
-    groupname = paste0(grouping_prefix, group)
-    odir = file.path(outputdir, groupname)
-    dir.create(odir, showWarnings = FALSE)
-
-    classes = as.character(obj@meta.data[[grouping]])
-    classes[classes != group] <- "_REST"
-    classes[classes == group] <- groupname
-    exprs = GetAssayData(obj)
-    tryCatch({
-        if (fgsea) {
-            runFGSEA(
-                prerank(exprs, groupname, "_REST", classes, method = prerank_method),
-                gmtfile,
-                top = top,
-                outdir = odir
+
+
+do_comparison <- function(object, caseinfo, subset_by, subset_val, group_by, group1, group2, prerank_method, plots, fgsea_args) {
+    log$info("  {group_by}: {group1} vs {group2} ...")
+    if (!is.null(group2)) {
+        # object <- subset(object, subset = !!sym(group_by) %in% c(group1, group2))
+        object <- tryCatch(
+            filter(object, !!sym(group_by) %in% c(group1, group2)),
+            error = function(e) NULL
+        )
+    }
+
+    if (!is.null(subset_by)) {
+        if (length(cases) == 1 && identical(caseinfo$name, subset_by)) {
+            # No need to show the case name in report
+            h1 <- paste0(subset_by, ": ", subset_val)
+            h2 <- paste0(group_by, ": ", group1, " vs ", group2 %||% "REST")
+            h3 <- "#"
+        } else {
+            h1 <- caseinfo$name
+            h2 <- paste0(subset_by, ": ", subset_val)
+            h3 <- paste0(group_by, ": ", group1, " vs ", group2 %||% "REST")
+        }
+        odir <- file.path(caseinfo$prefix, slugify(paste0(subset_by, "_", subset_val)))
+    } else if (length(cases) > 1) {
+        h1 <- caseinfo$name
+        h2 <- "#"
+        h3 <- paste0(group_by, ": ", group1, " vs ", group2 %||% "REST")
+        odir <- file.path(caseinfo$prefix, "No_Subsetting")
+    } else {
+        h1 <- paste0(group_by, ": ", group1, " vs ", group2 %||% "REST")
+        h2 <- "#"
+        h3 <- "#"
+        odir <- caseinfo$prefix
+    }
+
+    if (is.null(object) || ncol(object) < 10) {
+        msg <- paste0("  ! skipped. Groups together have less than 10 cells: ", group_by, " = ", group1, " vs ", group2)
+        log$warn(msg)
+        reporter$add(
+            list(kind = "error", content = msg),
+            h1 = h1,
+            h2 = h2,
+            h3 = h3
+        )
+        return(invisible())
+    }
+
+    classes <- as.character(object@meta.data[[group_by]])
+    if (!group1 %in% classes) {
+        stop("Group '", group1, "' not found in '", group_by, "' column of the Seurat object.")
+    }
+    if (!is.null(group2) && !group2 %in% classes) {
+        stop("Group '", group2, "' not found in '", group_by, "' column of the Seurat object.")
+    }
+    classes[classes != group1] <- "Other"
+    if (any(table(classes) < 5)) {
+        msg <- paste0(
+            "  ! skipped. Group has less than 5 cells: ",
+            paste(names(table(classes)[table(classes) < 5]), collapse = ", ")
+        )
+        log$warn(msg)
+
+        reporter$add(
+            list(kind = "error", content = msg),
+            h1 = h1,
+            h2 = h2,
+            h3 = h3
+        )
+        return(invisible())
+    }
+
+    features = intersect(rownames(object), metabolics)
+    ranks <- RunGSEAPreRank(
+        GetAssayData(object)[features, , drop = FALSE],
+        classes = object@meta.data[[group_by]],
+        case = group1,
+        control = group2,
+        method = prerank_method
+    )
+
+    fgsea_args <- fgsea_args %||% list()
+    fgsea_args$ranks <- ranks
+    fgsea_args$genesets <- pathways
+    fgsea_args$nproc <- fgsea_args$nproc %||% ncores
+    result <- do_call(RunGSEA, fgsea_args)
+
+    if (is.null(group2)) {
+        odir <- file.path(odir, slugify(paste0(group_by, "_", group1, "_vs_REST")))
+    } else {
+        odir <- file.path(odir, slugify(paste0(group_by, "_", group1, "_vs_", group2)))
+    }
+
+    dir.create(odir, showWarnings = FALSE, recursive = TRUE)
+    write.table(as.data.frame(result), file = file.path(odir, "fgsea_results.txt"), sep = "\t", quote = FALSE, row.names = FALSE)
+    write.table(data.frame(Gene = names(ranks), Rank = ranks), file = file.path(odir, "fgsea_ranks.txt"), sep = "\t", quote = FALSE, row.names = FALSE)
+
+    reporter$add(
+        list(kind = "descr", content = "A summary table of the GSEA results"),
+        list(kind = "table", src = file.path(odir, "fgsea_results.txt")),
+        h1 = h1,
+        h2 = h2,
+        h3 = h3
+    )
+
+    for (plot in names(plots)) {
+        plotargs <- plots[[plot]]
+        plotargs$level <- plotargs$level %||% "group"
+        if (plotargs$level != "group") { next }
+        plotargs$devpars <- plotargs$devpars %||% list()
+        plotargs$devpars$res <- plotargs$devpars$res %||% 100
+
+        if (identical(plotargs$plot_type, "summary")) {
+            p <- do_call(VizGSEA, c(list(result), plotargs))
+            plotprefix <- file.path(odir, slugify(plot))
+            plotargs$devpars$width <- plotargs$devpars$width %||% (attr(p, "width") * plotargs$devpars$res) %||% 800
+            plotargs$devpars$height <- plotargs$devpars$height %||% (attr(p, "height") * plotargs$devpars$res) %||% 600
+            png(
+                filename = paste0(plotprefix, ".png"),
+                width = plotargs$devpars$width,
+                height = plotargs$devpars$height,
+                res = plotargs$devpars$res
+            )
+            print(p)
+            dev.off()
+
+            reporter$add(
+                list(
+                    name = plot,
+                    contents = list(
+                        list(kind = "descr", content = plotargs$descr %||% plot),
+                        reporter$image(plotprefix, "png", FALSE, kind = "image")
+                    )
+                ),
+                h1 = h1,
+                h2 = h2,
+                h3 = h3,
+                ui = "tabs"
             )
         } else {
-            runGSEA(
-                exprs,
-                classes,
-                gmtfile,
-                odir
+            plotargs$combine = FALSE
+            plotargs$top_term = plotargs$top_term %||% 10
+            plotargs$gs <- result$pathway[1:plotargs$top_term]
+
+            ps <- do_call(VizGSEA, c(list(result), plotargs))
+            plotprefix <- file.path(odir, slugify(plot))
+            devpars <- plotargs$devpars
+            images <- list()
+            for (pname in names(ps)) {
+                p <- ps[[pname]]
+                devpars$width <- devpars$width %||% (attr(p, "width") * devpars$res) %||% 800
+                devpars$height <- devpars$height %||% (attr(p, "height") * devpars$res) %||% 600
+                prefix <- paste0(plotprefix, ".", slugify(pname))
+                images[[length(images) + 1]] <- reporter$image(prefix, c(), FALSE, kind = "table_image")
+                png(
+                    filename = paste0(prefix, ".png"),
+                    width = devpars$width,
+                    height = devpars$height,
+                    res = devpars$res
+                )
+                print(p)
+                dev.off()
+            }
+
+            reporter$add(
+                list(
+                    name = plot,
+                    ui = "table_of_images:2",
+                    contents = images
+                ),
+                h1 = h1,
+                h2 = h2,
+                h3 = h3,
+                ui = "tabs"
             )
         }
-    }, error=function(e) {
-        unlink(odir, recursive = T, force = T)
-        warning(paste("Unable to run for", group))
-        warning(e)
-    })
-
+    }
+    result$comparison <- paste0(group1, " vs ", group2 %||% "REST")
+    return(result)
 }
 
-do_one_subset <- function(s, subset_col, subset_prefix) {
-    print(paste("Processing subset", subset_col, ":", s))
-    if (is.null(s)) {
-        outputdir <- file.path(outdir, "ALL")
-        subset_obj <- sobj
+
+do_subset <- function(object, caseinfo, subset_by, subset_val, group_by, comparisons, prerank_method, plots, fgsea_args) {
+    if (!is.null(subset_by)) {
+        log$info("- Handling subset: {subset_by} = {subset_val} ...")
+        # object <- subset(object, subset = !!sym(subset_by) == subset_val)
+        object <- tryCatch(
+            filter(object, !!sym(subset_by) == subset_val & !is.na(!!sym(group_by))),
+            error = function(e) NULL
+        )
+
+        if (is.null(object) || ncol(object) < 5) {
+            if (length(cases) == 1 && identical(caseinfo$name, subset_by)) {
+                # No need to show case name in report
+                h1 <- paste0(subset_by, ": ", subset_val)
+                h2 <- "#"
+            } else {
+                h1 <- caseinfo$name
+                h2 <- paste0(subset_by, ": ", subset_val)
+            }
+
+            msg <- paste0("  ! skipped. Subset has less than 5 cells: ", subset_by, " = ", subset_val)
+            log$warn(msg)
+            reporter$add(list(kind = "error", content = msg), h1 = h1)
+            return(NULL)
+        }
+    }
+
+    groups <- unique(object@meta.data[[group_by]])
+    if (length(comparisons) == 0) {
+        result <- do_call(
+            rbind, lapply(
+                as.character(groups),
+                function(group) {
+                    do_comparison(object, caseinfo, subset_by, subset_val, group_by, group, NULL, prerank_method, plots, fgsea_args)
+                }
+            )
+        )
     } else {
-        outputdir <- file.path(outdir, paste0(subset_prefix, s))
-        subset_code <- paste0("subset(sobj, subset = ", subset_col, "=='", s, "')")
-        subset_obj <- eval(parse(text = subset_code))
+        result <- do_call(
+            rbind, lapply(
+                as.character(comparisons),
+                function(comparison) {
+                    if (grepl(":", comparison)) {
+                        group1 <- trimws(unlist(strsplit(comparison, ":")))
+                        group2 <- group1[2]
+                        group1 <- group1[1]
+                    } else {
+                        group1 <- comparison
+                        group2 <- NULL
+                    }
+                    do_comparison(object, caseinfo, subset_by, subset_val, group_by, group1, group2, prerank_method, plots, fgsea_args)
+                }
+            )
+        )
     }
-    dir.create(outputdir, showWarnings = FALSE)
 
-    subset_obj <- subset(subset_obj, features = intersect(rownames(subset_obj), metabolics))
+    result[["-log10(pval)"]] <- -log10(result$pval)
+    result[["-log10(padj)"]] <- -log10(result$padj)
 
-    groups = subset_obj@meta.data[[grouping]]
-    x = mclapply(as.character(unique(groups)), function(group) {
-        do_one_group(subset_obj, group, outputdir)
-    }, mc.cores = ncores)
-    if (any(unlist(lapply(x, class)) == "try-error")) {
-        stop("mclapply error")
+    odir <- NULL
+    if (!is.null(subset_by)) {
+        if (length(cases) == 1 && identical(caseinfo$name, subset_by)) {
+            # No need to show case name in report
+            h1 <- paste0(subset_by, ": ", subset_val)
+            h2 <- "Summary plots for all comparisons"
+            h3 <- "#"
+        } else {
+            h1 <- caseinfo$name
+            h2 <- paste0(subset_by, ": ", subset_val)
+            h3 <- "Summary plots for all comparisons"
+        }
+        odir <- file.path(caseinfo$prefix, slugify(paste0(subset_by, "_", subset_val)))
+    } else if (length(cases) > 1) {
+        h1 <- caseinfo$name
+        h2 <- "Summary plots for all comparisons"
+        h3 <- "#"
+        odir <- file.path(caseinfo$prefix, "No_Subsetting")
     }
-}
 
-do_one_subset_col <- function(subset_col, subset_prefix) {
-    if (is.null(subset_col)) {
-        do_one_subset(NULL, subset_col = NULL, subset_prefix = NULL)
+    if (!is.null(odir)) {
+        dir.create(odir, showWarnings = FALSE, recursive = TRUE)
+    }
+
+    for (plot in names(plots)) {
+        plotargs <- plots[[plot]]
+        plotargs$level <- plotargs$level %||% "group"
+        if (plotargs$level != "subset") { next }
+        if (is.null(odir)) {
+            stop("'subset_by' is NULL but plot level is 'subset': ", plot, ", use level = 'case' instead.")
+        }
+        plotargs$devpars <- plotargs$devpars %||% list()
+        plotargs$devpars$res <- plotargs$devpars$res %||% 100
+        plotargs$plot_type <- plotargs$plot_type %||% "dot"
+
+        if (identical(plotargs$plot_type, "dot")) {
+            plotargs$x <- plotargs$x %||% "comparison"
+            plotargs$y <- plotargs$y %||% "pathway"
+            plotargs$size_by <- plotargs$size_by %||% "NES"
+            plotargs$fill_by <- plotargs$fill_by %||% "-log10(padj)"
+            plotargs$fill_cutoff <- plotargs$fill_cutoff %||% -log10(0.05)
+            plotargs$fill_cutoff_name <- plotargs$fill_cutoff_name %||% "Insignificant"
+            plotargs$aspect.ratio <- plotargs$aspect.ratio %||% (length(unique(result$pathway)) / length(unique(result$comparison)) / 4)
+            plotargs$x_text_angle <- plotargs$x_text_angle %||% 90
+            p <- do_call(plotthis::DotPlot, c(list(result), plotargs))
+            plotprefix <- file.path(odir, slugify(plot))
+            plotargs$devpars$width <- plotargs$devpars$width %||% (attr(p, "width") * plotargs$devpars$res) %||% 800
+            plotargs$devpars$height <- plotargs$devpars$height %||% (attr(p, "height") * plotargs$devpars$res) %||% 600
+            png(
+                filename = paste0(plotprefix, ".png"),
+                width = plotargs$devpars$width,
+                height = plotargs$devpars$height,
+                res = plotargs$devpars$res
+            )
+            print(p)
+            dev.off()
+
+            reporter$add(
+                list(kind = "descr", content = plotargs$descr %||% plot),
+                reporter$image(plotprefix, "png", FALSE, kind = "image"),
+                h1 = h1,
+                h2 = h2,
+                h3 = h3
+            )
+        } else {
+            stop("`subset` level plot type not supported yet: ", plotargs$plot_type)
+        }
     }
-    subsets <- na.omit(unique(sobj@meta.data[[subset_col]]))
-    lapply(subsets, do_one_subset, subset_col = subset_col, subset_prefix = subset_prefix)
+    if (!is.null(subset_by)) {
+        result[[subset_by]] <- subset_val
+    }
+
+    return(result)
 }
 
-if (is.null(subsetting_cols)) {
-    do_one_subset_col(NULL)
-} else {
-    for (i in seq_along(subsetting_cols)) {
-        do_one_subset_col(subsetting_cols[i], subsetting_prefix[i])
+
+do_case <- function(casename) {
+    log$info("Processing case: {casename} ...")
+    case <- cases[[casename]]
+    caseinfo <- case_info(casename, outdir, create = TRUE)
+
+    if (is.null(case$subset_by)) {
+        result <- do_subset(
+            sobj,
+            caseinfo,
+            subset_by = NULL,
+            subset_val = NULL,
+            group_by = case$group_by,
+            comparisons = case$comparisons,
+            prerank_method = case$prerank_method,
+            plots = case$plots,
+            fgsea_args = case$fgsea_args
+        )
+    } else {
+        sobj_avail <- filter(sobj, !is.na(!!sym(case$subset_by)))
+        subsets <- if (is.factor(sobj_avail@meta.data[[case$subset_by]])) {
+            levels(sobj_avail@meta.data[[case$subset_by]])
+        } else {
+            unique(sobj_avail@meta.data[[case$subset_by]])
+        }
+        result <- do_call(
+            rbind, lapply(
+                as.character(subsets),
+                function(subset_val) {
+                    do_subset(
+                        sobj_avail,
+                        caseinfo,
+                        subset_by = case$subset_by,
+                        subset_val = subset_val,
+                        group_by = case$group_by,
+                        comparisons = case$comparisons,
+                        prerank_method = case$prerank_method,
+                        plots = case$plots,
+                        fgsea_args = case$fgsea_args
+                    )
+                }
+            )
+        )
+        result[[case$subset_by]] <- factor(result[[case$subset_by]], levels = subsets)
+    }
+    result$pathway <- factor(result$pathway, levels = pathway_names)
+
+    if (!is.null(case$subset_by)) {
+        if (length(cases) == 1 && identical(caseinfo$name, case$subset_by)) {
+            h1 <- "Summary plots for all subsets"
+            h2 <- "#"
+        } else {
+            h1 <- caseinfo$name
+            h2 <- "Summary plots for all subsets"
+        }
+    } else if (length(cases) > 1) {
+        h1 <- caseinfo$name
+        h2 <- "Summary plots for all comparisons"
+    } else {
+        h1 <- "Summary plots for all comparisons"
+        h2 <- "#"
+    }
+
+    for (plot in names(plots)) {
+        plotargs <- plots[[plot]]
+        plotargs$level <- plotargs$level %||% "group"
+        if (plotargs$level != "case") { next }
+        plotargs$devpars <- plotargs$devpars %||% list()
+        plotargs$devpars$res <- plotargs$devpars$res %||% 100
+        plotargs$plot_type <- plotargs$plot_type %||% "dot"
+
+        if (identical(plotargs$plot_type, "dot")) {
+            plotargs$x <- plotargs$x %||% "comparison"
+            plotargs$y <- plotargs$y %||% "pathway"
+            plotargs$size_by <- plotargs$size_by %||% "NES"
+            plotargs$fill_by <- plotargs$fill_by %||% "-log10(padj)"
+            plotargs$fill_cutoff <- plotargs$fill_cutoff %||% -log10(0.05)
+            plotargs$fill_cutoff_name <- plotargs$fill_cutoff_name %||% "Insignificant"
+            plotargs$aspect.ratio <- plotargs$aspect.ratio %||% (length(unique(result$pathway)) / length(unique(result$comparison)) / 4)
+            plotargs$x_text_angle <- plotargs$x_text_angle %||% 90
+            if (!is.null(subset_by)) {
+                plotargs$split_by <- plotargs$split_by %||% subset_by
+            }
+            p <- do_call(plotthis::DotPlot, c(list(result), plotargs))
+            plotprefix <- file.path(caseinfo$prefix, slugify(plot))
+            plotargs$devpars$width <- plotargs$devpars$width %||% (attr(p, "width") * plotargs$devpars$res) %||% 800
+            plotargs$devpars$height <- plotargs$devpars$height %||% (attr(p, "height") * plotargs$devpars$res) %||% 600
+            png(
+                filename = paste0(plotprefix, ".png"),
+                width = plotargs$devpars$width,
+                height = plotargs$devpars$height,
+                res = plotargs$devpars$res
+            )
+            print(p)
+            dev.off()
+
+            reporter$add(
+                list(kind = "descr", content = plotargs$descr %||% plot),
+                reporter$image(plotprefix, "png", FALSE, kind = "image"),
+                h1 = h1,
+                h2 = h2
+            )
+        } else {
+            stop("`case` level plot type not supported yet: ", plotargs$plot_type)
+        }
     }
 }
 
+sapply(names(cases), do_case)
+
+reporter$save(dirname(outdir))