bio-velvet_underground 0.0.1
Sign up to get free protection for your applications and to get access to all the features.
- checksums.yaml +7 -0
- data/.document +5 -0
- data/.gitmodules +3 -0
- data/.travis.yml +13 -0
- data/Gemfile +19 -0
- data/LICENSE.txt +20 -0
- data/README.md +53 -0
- data/Rakefile +51 -0
- data/VERSION +1 -0
- data/ext/bioruby.patch +60 -0
- data/ext/mkrf_conf.rb +50 -0
- data/ext/src/Makefile +125 -0
- data/ext/src/src/allocArray.c +305 -0
- data/ext/src/src/allocArray.h +86 -0
- data/ext/src/src/autoOpen.c +107 -0
- data/ext/src/src/autoOpen.h +18 -0
- data/ext/src/src/binarySequences.c +813 -0
- data/ext/src/src/binarySequences.h +125 -0
- data/ext/src/src/concatenatedGraph.c +233 -0
- data/ext/src/src/concatenatedGraph.h +30 -0
- data/ext/src/src/concatenatedPreGraph.c +262 -0
- data/ext/src/src/concatenatedPreGraph.h +29 -0
- data/ext/src/src/correctedGraph.c +2642 -0
- data/ext/src/src/correctedGraph.h +32 -0
- data/ext/src/src/dfib.c +509 -0
- data/ext/src/src/dfib.h +69 -0
- data/ext/src/src/dfibHeap.c +89 -0
- data/ext/src/src/dfibHeap.h +39 -0
- data/ext/src/src/dfibpriv.h +105 -0
- data/ext/src/src/fib.c +628 -0
- data/ext/src/src/fib.h +78 -0
- data/ext/src/src/fibHeap.c +79 -0
- data/ext/src/src/fibHeap.h +41 -0
- data/ext/src/src/fibpriv.h +110 -0
- data/ext/src/src/globals.h +153 -0
- data/ext/src/src/graph.c +3983 -0
- data/ext/src/src/graph.h +233 -0
- data/ext/src/src/graphReConstruction.c +1472 -0
- data/ext/src/src/graphReConstruction.h +30 -0
- data/ext/src/src/graphStats.c +2167 -0
- data/ext/src/src/graphStats.h +72 -0
- data/ext/src/src/kmer.c +652 -0
- data/ext/src/src/kmer.h +73 -0
- data/ext/src/src/kmerOccurenceTable.c +236 -0
- data/ext/src/src/kmerOccurenceTable.h +44 -0
- data/ext/src/src/kseq.h +223 -0
- data/ext/src/src/locallyCorrectedGraph.c +557 -0
- data/ext/src/src/locallyCorrectedGraph.h +40 -0
- data/ext/src/src/passageMarker.c +677 -0
- data/ext/src/src/passageMarker.h +137 -0
- data/ext/src/src/preGraph.c +1717 -0
- data/ext/src/src/preGraph.h +106 -0
- data/ext/src/src/preGraphConstruction.c +990 -0
- data/ext/src/src/preGraphConstruction.h +26 -0
- data/ext/src/src/readCoherentGraph.c +557 -0
- data/ext/src/src/readCoherentGraph.h +30 -0
- data/ext/src/src/readSet.c +1734 -0
- data/ext/src/src/readSet.h +67 -0
- data/ext/src/src/recycleBin.c +199 -0
- data/ext/src/src/recycleBin.h +58 -0
- data/ext/src/src/roadMap.c +342 -0
- data/ext/src/src/roadMap.h +65 -0
- data/ext/src/src/run.c +318 -0
- data/ext/src/src/run.h +52 -0
- data/ext/src/src/run2.c +712 -0
- data/ext/src/src/scaffold.c +1876 -0
- data/ext/src/src/scaffold.h +64 -0
- data/ext/src/src/shortReadPairs.c +1243 -0
- data/ext/src/src/shortReadPairs.h +32 -0
- data/ext/src/src/splay.c +259 -0
- data/ext/src/src/splay.h +43 -0
- data/ext/src/src/splayTable.c +1315 -0
- data/ext/src/src/splayTable.h +31 -0
- data/ext/src/src/tightString.c +362 -0
- data/ext/src/src/tightString.h +82 -0
- data/ext/src/src/utility.c +199 -0
- data/ext/src/src/utility.h +98 -0
- data/ext/src/third-party/zlib-1.2.3/ChangeLog +855 -0
- data/ext/src/third-party/zlib-1.2.3/FAQ +339 -0
- data/ext/src/third-party/zlib-1.2.3/INDEX +51 -0
- data/ext/src/third-party/zlib-1.2.3/Makefile +154 -0
- data/ext/src/third-party/zlib-1.2.3/Makefile.in +154 -0
- data/ext/src/third-party/zlib-1.2.3/README +125 -0
- data/ext/src/third-party/zlib-1.2.3/adler32.c +149 -0
- data/ext/src/third-party/zlib-1.2.3/algorithm.txt +209 -0
- data/ext/src/third-party/zlib-1.2.3/amiga/Makefile.pup +66 -0
- data/ext/src/third-party/zlib-1.2.3/amiga/Makefile.sas +65 -0
- data/ext/src/third-party/zlib-1.2.3/as400/bndsrc +132 -0
- data/ext/src/third-party/zlib-1.2.3/as400/compile.clp +123 -0
- data/ext/src/third-party/zlib-1.2.3/as400/readme.txt +111 -0
- data/ext/src/third-party/zlib-1.2.3/as400/zlib.inc +331 -0
- data/ext/src/third-party/zlib-1.2.3/compress.c +79 -0
- data/ext/src/third-party/zlib-1.2.3/configure +459 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/README.contrib +71 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/ada/buffer_demo.adb +106 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/ada/mtest.adb +156 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/ada/read.adb +156 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/ada/readme.txt +65 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/ada/test.adb +463 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/ada/zlib-streams.adb +225 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/ada/zlib-streams.ads +114 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/ada/zlib-thin.adb +141 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/ada/zlib-thin.ads +450 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/ada/zlib.adb +701 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/ada/zlib.ads +328 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/ada/zlib.gpr +20 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/asm586/README.586 +43 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/asm586/match.S +364 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/asm686/README.686 +34 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/asm686/match.S +329 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/blast/Makefile +8 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/blast/README +4 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/blast/blast.c +444 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/blast/blast.h +71 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/blast/test.pk +0 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/blast/test.txt +1 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/delphi/ZLib.pas +557 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/delphi/ZLibConst.pas +11 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/delphi/readme.txt +76 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/delphi/zlibd32.mak +93 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/dotzlib/DotZLib.build +33 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/dotzlib/DotZLib.chm +0 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/dotzlib/DotZLib.sln +21 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/dotzlib/DotZLib/AssemblyInfo.cs +58 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/dotzlib/DotZLib/ChecksumImpl.cs +202 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/dotzlib/DotZLib/CircularBuffer.cs +83 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/dotzlib/DotZLib/CodecBase.cs +198 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/dotzlib/DotZLib/Deflater.cs +106 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/dotzlib/DotZLib/DotZLib.cs +288 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/dotzlib/DotZLib/DotZLib.csproj +141 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/dotzlib/DotZLib/GZipStream.cs +301 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/dotzlib/DotZLib/Inflater.cs +105 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/dotzlib/DotZLib/UnitTests.cs +274 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/dotzlib/LICENSE_1_0.txt +23 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/dotzlib/readme.txt +58 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/infback9/README +1 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/infback9/infback9.c +608 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/infback9/infback9.h +37 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/infback9/inffix9.h +107 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/infback9/inflate9.h +47 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/infback9/inftree9.c +323 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/infback9/inftree9.h +55 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/inflate86/inffas86.c +1157 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/inflate86/inffast.S +1368 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/iostream/test.cpp +24 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/iostream/zfstream.cpp +329 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/iostream/zfstream.h +128 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/iostream2/zstream.h +307 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/iostream2/zstream_test.cpp +25 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/iostream3/README +35 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/iostream3/TODO +17 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/iostream3/test.cc +50 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/iostream3/zfstream.cc +479 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/iostream3/zfstream.h +466 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/masm686/match.asm +413 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/masmx64/bld_ml64.bat +2 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/masmx64/gvmat64.asm +513 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/masmx64/gvmat64.obj +0 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/masmx64/inffas8664.c +186 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/masmx64/inffasx64.asm +392 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/masmx64/inffasx64.obj +0 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/masmx64/readme.txt +28 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/masmx86/bld_ml32.bat +2 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/masmx86/gvmat32.asm +972 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/masmx86/gvmat32.obj +0 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/masmx86/gvmat32c.c +62 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/masmx86/inffas32.asm +1083 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/masmx86/inffas32.obj +0 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/masmx86/mkasm.bat +3 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/masmx86/readme.txt +21 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/minizip/ChangeLogUnzip +67 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/minizip/Makefile +25 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/minizip/crypt.h +132 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/minizip/ioapi.c +177 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/minizip/ioapi.h +75 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/minizip/iowin32.c +270 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/minizip/iowin32.h +21 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/minizip/miniunz.c +585 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/minizip/minizip.c +420 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/minizip/mztools.c +281 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/minizip/mztools.h +31 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/minizip/unzip.c +1598 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/minizip/unzip.h +354 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/minizip/zip.c +1219 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/minizip/zip.h +235 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/pascal/example.pas +599 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/pascal/readme.txt +76 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/pascal/zlibd32.mak +93 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/pascal/zlibpas.pas +236 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/puff/Makefile +8 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/puff/README +63 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/puff/puff.c +837 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/puff/puff.h +31 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/puff/zeros.raw +0 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/testzlib/testzlib.c +275 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/testzlib/testzlib.txt +10 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/untgz/Makefile +14 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/untgz/Makefile.msc +17 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/untgz/untgz.c +674 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/vstudio/readme.txt +73 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/vstudio/vc7/miniunz.vcproj +126 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/vstudio/vc7/minizip.vcproj +126 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/vstudio/vc7/testzlib.vcproj +126 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/vstudio/vc7/zlib.rc +32 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/vstudio/vc7/zlibstat.vcproj +246 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/vstudio/vc7/zlibvc.def +92 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/vstudio/vc7/zlibvc.sln +78 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/vstudio/vc7/zlibvc.vcproj +445 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/vstudio/vc8/miniunz.vcproj +566 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/vstudio/vc8/minizip.vcproj +563 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/vstudio/vc8/testzlib.vcproj +948 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/vstudio/vc8/testzlibdll.vcproj +567 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/vstudio/vc8/zlib.rc +32 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/vstudio/vc8/zlibstat.vcproj +870 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/vstudio/vc8/zlibvc.def +92 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/vstudio/vc8/zlibvc.sln +144 -0
- data/ext/src/third-party/zlib-1.2.3/contrib/vstudio/vc8/zlibvc.vcproj +1219 -0
- data/ext/src/third-party/zlib-1.2.3/crc32.c +423 -0
- data/ext/src/third-party/zlib-1.2.3/crc32.h +441 -0
- data/ext/src/third-party/zlib-1.2.3/deflate.c +1736 -0
- data/ext/src/third-party/zlib-1.2.3/deflate.h +331 -0
- data/ext/src/third-party/zlib-1.2.3/example.c +565 -0
- data/ext/src/third-party/zlib-1.2.3/examples/README.examples +42 -0
- data/ext/src/third-party/zlib-1.2.3/examples/fitblk.c +233 -0
- data/ext/src/third-party/zlib-1.2.3/examples/gun.c +693 -0
- data/ext/src/third-party/zlib-1.2.3/examples/gzappend.c +500 -0
- data/ext/src/third-party/zlib-1.2.3/examples/gzjoin.c +448 -0
- data/ext/src/third-party/zlib-1.2.3/examples/gzlog.c +413 -0
- data/ext/src/third-party/zlib-1.2.3/examples/gzlog.h +58 -0
- data/ext/src/third-party/zlib-1.2.3/examples/zlib_how.html +523 -0
- data/ext/src/third-party/zlib-1.2.3/examples/zpipe.c +191 -0
- data/ext/src/third-party/zlib-1.2.3/examples/zran.c +404 -0
- data/ext/src/third-party/zlib-1.2.3/gzio.c +1026 -0
- data/ext/src/third-party/zlib-1.2.3/infback.c +623 -0
- data/ext/src/third-party/zlib-1.2.3/inffast.c +318 -0
- data/ext/src/third-party/zlib-1.2.3/inffast.h +11 -0
- data/ext/src/third-party/zlib-1.2.3/inffixed.h +94 -0
- data/ext/src/third-party/zlib-1.2.3/inflate.c +1368 -0
- data/ext/src/third-party/zlib-1.2.3/inflate.h +115 -0
- data/ext/src/third-party/zlib-1.2.3/inftrees.c +329 -0
- data/ext/src/third-party/zlib-1.2.3/inftrees.h +55 -0
- data/ext/src/third-party/zlib-1.2.3/make_vms.com +461 -0
- data/ext/src/third-party/zlib-1.2.3/minigzip.c +322 -0
- data/ext/src/third-party/zlib-1.2.3/msdos/Makefile.bor +109 -0
- data/ext/src/third-party/zlib-1.2.3/msdos/Makefile.dj2 +104 -0
- data/ext/src/third-party/zlib-1.2.3/msdos/Makefile.emx +69 -0
- data/ext/src/third-party/zlib-1.2.3/msdos/Makefile.msc +106 -0
- data/ext/src/third-party/zlib-1.2.3/msdos/Makefile.tc +94 -0
- data/ext/src/third-party/zlib-1.2.3/old/Makefile.riscos +151 -0
- data/ext/src/third-party/zlib-1.2.3/old/README +3 -0
- data/ext/src/third-party/zlib-1.2.3/old/descrip.mms +48 -0
- data/ext/src/third-party/zlib-1.2.3/old/os2/Makefile.os2 +136 -0
- data/ext/src/third-party/zlib-1.2.3/old/os2/zlib.def +51 -0
- data/ext/src/third-party/zlib-1.2.3/old/visual-basic.txt +160 -0
- data/ext/src/third-party/zlib-1.2.3/old/zlib.html +971 -0
- data/ext/src/third-party/zlib-1.2.3/projects/README.projects +41 -0
- data/ext/src/third-party/zlib-1.2.3/projects/visualc6/README.txt +73 -0
- data/ext/src/third-party/zlib-1.2.3/projects/visualc6/example.dsp +278 -0
- data/ext/src/third-party/zlib-1.2.3/projects/visualc6/minigzip.dsp +278 -0
- data/ext/src/third-party/zlib-1.2.3/projects/visualc6/zlib.dsp +609 -0
- data/ext/src/third-party/zlib-1.2.3/projects/visualc6/zlib.dsw +59 -0
- data/ext/src/third-party/zlib-1.2.3/qnx/package.qpg +141 -0
- data/ext/src/third-party/zlib-1.2.3/trees.c +1219 -0
- data/ext/src/third-party/zlib-1.2.3/trees.h +128 -0
- data/ext/src/third-party/zlib-1.2.3/uncompr.c +61 -0
- data/ext/src/third-party/zlib-1.2.3/win32/DLL_FAQ.txt +397 -0
- data/ext/src/third-party/zlib-1.2.3/win32/Makefile.bor +107 -0
- data/ext/src/third-party/zlib-1.2.3/win32/Makefile.emx +69 -0
- data/ext/src/third-party/zlib-1.2.3/win32/Makefile.gcc +141 -0
- data/ext/src/third-party/zlib-1.2.3/win32/Makefile.msc +126 -0
- data/ext/src/third-party/zlib-1.2.3/win32/VisualC.txt +3 -0
- data/ext/src/third-party/zlib-1.2.3/win32/zlib.def +60 -0
- data/ext/src/third-party/zlib-1.2.3/win32/zlib1.rc +39 -0
- data/ext/src/third-party/zlib-1.2.3/zconf.h +332 -0
- data/ext/src/third-party/zlib-1.2.3/zconf.in.h +332 -0
- data/ext/src/third-party/zlib-1.2.3/zlib.3 +159 -0
- data/ext/src/third-party/zlib-1.2.3/zlib.h +1357 -0
- data/ext/src/third-party/zlib-1.2.3/zutil.c +318 -0
- data/ext/src/third-party/zlib-1.2.3/zutil.h +269 -0
- data/lib/bio-velvet_underground.rb +12 -0
- data/lib/bio-velvet_underground/external/VERSION +1 -0
- data/lib/bio-velvet_underground/velvet_underground.rb +72 -0
- data/spec/binary_sequence_store_spec.rb +27 -0
- data/spec/data/1/CnyUnifiedSeq +0 -0
- data/spec/spec_helper.rb +31 -0
- metadata +456 -0
data/ext/src/src/graph.c
ADDED
@@ -0,0 +1,3983 @@
|
|
1
|
+
/*
|
2
|
+
Copyright 2007, 2008 Daniel Zerbino (zerbino@ebi.ac.uk)
|
3
|
+
|
4
|
+
This file is part of Velvet.
|
5
|
+
|
6
|
+
Velvet is free software; you can redistribute it and/or modify
|
7
|
+
it under the terms of the GNU General Public License as published by
|
8
|
+
the Free Software Foundation; either version 2 of the License, or
|
9
|
+
(at your option) any later version.
|
10
|
+
|
11
|
+
Velvet is distributed in the hope that it will be useful,
|
12
|
+
but WITHOUT ANY WARRANTY; without even the implied warranty of
|
13
|
+
MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
|
14
|
+
GNU General Public License for more details.
|
15
|
+
|
16
|
+
You should have received a copy of the GNU General Public License
|
17
|
+
along with Velvet; if not, write to the Free Software
|
18
|
+
Foundation, Inc., 51 Franklin St, Fifth Floor, Boston, MA 02110-1301 USA
|
19
|
+
|
20
|
+
*/
|
21
|
+
#include <stdlib.h>
|
22
|
+
#include <stdio.h>
|
23
|
+
#include <string.h>
|
24
|
+
|
25
|
+
#include "globals.h"
|
26
|
+
#include "graph.h"
|
27
|
+
#include "recycleBin.h"
|
28
|
+
#include "tightString.h"
|
29
|
+
#include "passageMarker.h"
|
30
|
+
#include "utility.h"
|
31
|
+
#include "kmer.h"
|
32
|
+
|
33
|
+
#define ADENINE 0
|
34
|
+
#define CYTOSINE 1
|
35
|
+
#define GUANINE 2
|
36
|
+
#define THYMINE 3
|
37
|
+
|
38
|
+
struct arc_st {
|
39
|
+
Arc *twinArc; // 64
|
40
|
+
Arc *next; // 64
|
41
|
+
Arc *previous; // 64
|
42
|
+
Arc *nextInLookupTable; // 64
|
43
|
+
Node *destination; // 64
|
44
|
+
IDnum multiplicity; // 32
|
45
|
+
} ATTRIBUTE_PACKED; // 352 Total
|
46
|
+
|
47
|
+
struct node_st {
|
48
|
+
Node *twinNode; // 64
|
49
|
+
Arc *arc; // 64
|
50
|
+
Descriptor *descriptor; // 64
|
51
|
+
PassageMarkerI marker; // 32
|
52
|
+
IDnum length; // 32
|
53
|
+
#ifndef SINGLE_COV_CAT
|
54
|
+
IDnum virtualCoverage[CATEGORIES]; // 32 * 2
|
55
|
+
IDnum originalVirtualCoverage[CATEGORIES]; // 32 * 2
|
56
|
+
#else
|
57
|
+
IDnum virtualCoverage; // 32 * 2
|
58
|
+
#endif
|
59
|
+
IDnum ID; // 32
|
60
|
+
IDnum arcCount; // 32
|
61
|
+
boolean status; // 1
|
62
|
+
boolean uniqueness; // 1
|
63
|
+
} ATTRIBUTE_PACKED; // 418 Total
|
64
|
+
|
65
|
+
struct shortReadMarker_st {
|
66
|
+
IDnum position;
|
67
|
+
IDnum readID;
|
68
|
+
ShortLength offset;
|
69
|
+
} ATTRIBUTE_PACKED;
|
70
|
+
|
71
|
+
struct gapMarker_st {
|
72
|
+
GapMarker *next;
|
73
|
+
IDnum position;
|
74
|
+
IDnum length;
|
75
|
+
} ATTRIBUTE_PACKED;
|
76
|
+
|
77
|
+
struct graph_st {
|
78
|
+
Node **nodes;
|
79
|
+
Arc **arcLookupTable;
|
80
|
+
ShortReadMarker **nodeReads;
|
81
|
+
IDnum *nodeReadCounts;
|
82
|
+
GapMarker **gapMarkers;
|
83
|
+
Coordinate insertLengths[CATEGORIES + 1];
|
84
|
+
double insertLengths_var[CATEGORIES + 1];
|
85
|
+
IDnum sequenceCount;
|
86
|
+
IDnum nodeCount;
|
87
|
+
int wordLength;
|
88
|
+
boolean double_stranded;
|
89
|
+
};
|
90
|
+
|
91
|
+
static RecycleBin *arcMemory = NULL;
|
92
|
+
static RecycleBin *nodeMemory = NULL;
|
93
|
+
static RecycleBin *gapMarkerMemory = NULL;
|
94
|
+
|
95
|
+
#define BLOCKSIZE 50
|
96
|
+
#define GAPBLOCKSIZE 10000
|
97
|
+
|
98
|
+
Arc *allocateArc()
|
99
|
+
{
|
100
|
+
if (arcMemory == NULL)
|
101
|
+
arcMemory = newRecycleBin(sizeof(Arc), BLOCKSIZE);
|
102
|
+
|
103
|
+
return allocatePointer(arcMemory);
|
104
|
+
}
|
105
|
+
|
106
|
+
void deallocateArc(Arc * arc)
|
107
|
+
{
|
108
|
+
deallocatePointer(arcMemory, arc);
|
109
|
+
}
|
110
|
+
|
111
|
+
Node *allocateNode()
|
112
|
+
{
|
113
|
+
if (nodeMemory == NULL)
|
114
|
+
nodeMemory = newRecycleBin(sizeof(Node), BLOCKSIZE);
|
115
|
+
|
116
|
+
return (Node *) allocatePointer(nodeMemory);
|
117
|
+
}
|
118
|
+
|
119
|
+
void deallocateNode(Node * node)
|
120
|
+
{
|
121
|
+
deallocatePointer(nodeMemory, node);
|
122
|
+
}
|
123
|
+
|
124
|
+
// Returns the twin node of a given node
|
125
|
+
Node *getTwinNode(Node * node)
|
126
|
+
{
|
127
|
+
return node->twinNode;
|
128
|
+
}
|
129
|
+
|
130
|
+
// Inserts new passage marker in the marker list of destination node
|
131
|
+
void insertPassageMarker(PassageMarkerI marker, Node * destination)
|
132
|
+
{
|
133
|
+
setTopOfTheNode(marker);
|
134
|
+
setNextInNode(marker, destination->marker);
|
135
|
+
destination->marker = marker;
|
136
|
+
}
|
137
|
+
|
138
|
+
// Returns the length of the node's descriptor list
|
139
|
+
Coordinate getNodeLength(Node * node)
|
140
|
+
{
|
141
|
+
return node->length;
|
142
|
+
}
|
143
|
+
|
144
|
+
// Returns the number of nodes in the graph
|
145
|
+
IDnum nodeCount(Graph * graph)
|
146
|
+
{
|
147
|
+
return graph->nodeCount;
|
148
|
+
}
|
149
|
+
|
150
|
+
// returns the number of sequences used to buid the graph
|
151
|
+
IDnum sequenceCount(Graph * graph)
|
152
|
+
{
|
153
|
+
return graph->sequenceCount;
|
154
|
+
}
|
155
|
+
|
156
|
+
// Creates an arc from node origin to node destination.
|
157
|
+
// If this arc already exists, increments its multiplicity by 1.
|
158
|
+
Arc *createArc(Node * originNode, Node * destinationNode, Graph * graph)
|
159
|
+
{
|
160
|
+
Arc *arc, *twinArc;
|
161
|
+
Node *destinationTwin;
|
162
|
+
IDnum lookupIndex;
|
163
|
+
|
164
|
+
if (originNode == NULL || destinationNode == NULL)
|
165
|
+
return NULL;
|
166
|
+
|
167
|
+
// velvetLog("Connecting nodes %i -> %i\n", originNode->ID, destinationNode->ID);
|
168
|
+
|
169
|
+
arc = getArcBetweenNodes(originNode, destinationNode, graph);
|
170
|
+
|
171
|
+
if (arc != NULL) {
|
172
|
+
arc->multiplicity++;
|
173
|
+
arc->twinArc->multiplicity++;
|
174
|
+
return arc;
|
175
|
+
}
|
176
|
+
// If not found
|
177
|
+
#ifdef _OPENMP
|
178
|
+
#pragma omp critical
|
179
|
+
#endif
|
180
|
+
arc = allocateArc();
|
181
|
+
arc->destination = destinationNode;
|
182
|
+
arc->multiplicity = 1;
|
183
|
+
arc->previous = NULL;
|
184
|
+
arc->next = originNode->arc;
|
185
|
+
if (originNode->arc != NULL)
|
186
|
+
originNode->arc->previous = arc;
|
187
|
+
originNode->arc = arc;
|
188
|
+
originNode->arcCount++;
|
189
|
+
|
190
|
+
destinationTwin = destinationNode->twinNode;
|
191
|
+
|
192
|
+
// Hairpin case
|
193
|
+
if (destinationTwin == originNode) {
|
194
|
+
arc->multiplicity++;
|
195
|
+
arc->twinArc = arc;
|
196
|
+
if (graph->arcLookupTable != NULL) {
|
197
|
+
lookupIndex =
|
198
|
+
2 * originNode->ID + destinationNode->ID +
|
199
|
+
3 * graph->nodeCount;
|
200
|
+
arc->nextInLookupTable =
|
201
|
+
graph->arcLookupTable[lookupIndex];
|
202
|
+
graph->arcLookupTable[lookupIndex] = arc;
|
203
|
+
}
|
204
|
+
return arc;
|
205
|
+
}
|
206
|
+
|
207
|
+
#ifdef _OPENMP
|
208
|
+
#pragma omp critical
|
209
|
+
#endif
|
210
|
+
twinArc = allocateArc();
|
211
|
+
twinArc->destination = originNode->twinNode;
|
212
|
+
twinArc->multiplicity = 1;
|
213
|
+
twinArc->previous = NULL;
|
214
|
+
twinArc->next = destinationTwin->arc;
|
215
|
+
if (destinationTwin->arc != NULL)
|
216
|
+
destinationTwin->arc->previous = twinArc;
|
217
|
+
destinationTwin->arc = twinArc;
|
218
|
+
destinationTwin->arcCount++;
|
219
|
+
|
220
|
+
arc->twinArc = twinArc;
|
221
|
+
twinArc->twinArc = arc;
|
222
|
+
|
223
|
+
if (graph->arcLookupTable != NULL) {
|
224
|
+
lookupIndex =
|
225
|
+
2 * originNode->ID + destinationNode->ID +
|
226
|
+
3 * graph->nodeCount;
|
227
|
+
arc->nextInLookupTable =
|
228
|
+
graph->arcLookupTable[lookupIndex];
|
229
|
+
graph->arcLookupTable[lookupIndex] = arc;
|
230
|
+
|
231
|
+
lookupIndex =
|
232
|
+
-2 * destinationNode->ID - originNode->ID +
|
233
|
+
3 * graph->nodeCount;
|
234
|
+
twinArc->nextInLookupTable =
|
235
|
+
graph->arcLookupTable[lookupIndex];
|
236
|
+
graph->arcLookupTable[lookupIndex] = twinArc;
|
237
|
+
}
|
238
|
+
return arc;
|
239
|
+
}
|
240
|
+
|
241
|
+
void createAnalogousArc(Node * originNode, Node * destinationNode,
|
242
|
+
Arc * refArc, Graph * graph)
|
243
|
+
{
|
244
|
+
Arc *arc, *twinArc;
|
245
|
+
Node *destinationTwin;
|
246
|
+
IDnum lookupIndex;
|
247
|
+
|
248
|
+
if (originNode == NULL || destinationNode == NULL)
|
249
|
+
return;
|
250
|
+
|
251
|
+
// velvetLog("Connecting nodes %i -> %i\n", originNode->ID, destinationNode->ID);
|
252
|
+
|
253
|
+
arc = getArcBetweenNodes(originNode, destinationNode, graph);
|
254
|
+
|
255
|
+
if (arc != NULL) {
|
256
|
+
if (refArc->twinArc != refArc) {
|
257
|
+
arc->multiplicity += getMultiplicity(refArc);
|
258
|
+
arc->twinArc->multiplicity +=
|
259
|
+
getMultiplicity(refArc);
|
260
|
+
} else {
|
261
|
+
arc->multiplicity += getMultiplicity(refArc) / 2;
|
262
|
+
arc->twinArc->multiplicity +=
|
263
|
+
getMultiplicity(refArc) / 2;
|
264
|
+
}
|
265
|
+
return;
|
266
|
+
}
|
267
|
+
// If not found
|
268
|
+
arc = allocateArc();
|
269
|
+
arc->destination = destinationNode;
|
270
|
+
arc->multiplicity = getMultiplicity(refArc);
|
271
|
+
arc->previous = NULL;
|
272
|
+
arc->next = originNode->arc;
|
273
|
+
if (originNode->arc != NULL)
|
274
|
+
originNode->arc->previous = arc;
|
275
|
+
originNode->arc = arc;
|
276
|
+
originNode->arcCount++;
|
277
|
+
|
278
|
+
destinationTwin = destinationNode->twinNode;
|
279
|
+
|
280
|
+
// Hairpin case
|
281
|
+
if (destinationTwin == originNode) {
|
282
|
+
arc->twinArc = arc;
|
283
|
+
if (refArc->twinArc != refArc)
|
284
|
+
arc->multiplicity *= 2;
|
285
|
+
|
286
|
+
if (graph->arcLookupTable != NULL) {
|
287
|
+
lookupIndex =
|
288
|
+
2 * originNode->ID + destinationNode->ID
|
289
|
+
+ 3 * graph->nodeCount;
|
290
|
+
arc->nextInLookupTable =
|
291
|
+
graph->arcLookupTable[lookupIndex];
|
292
|
+
graph->arcLookupTable[lookupIndex] = arc;
|
293
|
+
}
|
294
|
+
return;
|
295
|
+
}
|
296
|
+
|
297
|
+
twinArc = allocateArc();
|
298
|
+
twinArc->destination = originNode->twinNode;
|
299
|
+
twinArc->multiplicity = getMultiplicity(refArc);
|
300
|
+
twinArc->previous = NULL;
|
301
|
+
twinArc->next = destinationTwin->arc;
|
302
|
+
if (destinationTwin->arc != NULL)
|
303
|
+
destinationTwin->arc->previous = twinArc;
|
304
|
+
destinationTwin->arc = twinArc;
|
305
|
+
destinationTwin->arcCount++;
|
306
|
+
|
307
|
+
arc->twinArc = twinArc;
|
308
|
+
twinArc->twinArc = arc;
|
309
|
+
|
310
|
+
if (graph->arcLookupTable != NULL) {
|
311
|
+
lookupIndex =
|
312
|
+
2 * originNode->ID + destinationNode->ID +
|
313
|
+
3 * graph->nodeCount;
|
314
|
+
arc->nextInLookupTable =
|
315
|
+
graph->arcLookupTable[lookupIndex];
|
316
|
+
graph->arcLookupTable[lookupIndex] = arc;
|
317
|
+
|
318
|
+
lookupIndex =
|
319
|
+
-2 * destinationNode->ID - originNode->ID +
|
320
|
+
3 * graph->nodeCount;
|
321
|
+
twinArc->nextInLookupTable =
|
322
|
+
graph->arcLookupTable[lookupIndex];
|
323
|
+
graph->arcLookupTable[lookupIndex] = twinArc;
|
324
|
+
}
|
325
|
+
}
|
326
|
+
|
327
|
+
Arc *getArcBetweenNodes(Node * originNode, Node * destinationNode,
|
328
|
+
Graph * graph)
|
329
|
+
{
|
330
|
+
Arc *arc;
|
331
|
+
Node *twinDestination, *twinOrigin;
|
332
|
+
|
333
|
+
if (originNode == NULL || destinationNode == NULL)
|
334
|
+
return NULL;
|
335
|
+
|
336
|
+
if (graph->arcLookupTable != NULL) {
|
337
|
+
for (arc =
|
338
|
+
graph->arcLookupTable[2 * originNode->ID +
|
339
|
+
destinationNode->ID +
|
340
|
+
3 * graph->nodeCount];
|
341
|
+
arc != NULL; arc = arc->nextInLookupTable) {
|
342
|
+
if (arc->destination == destinationNode) {
|
343
|
+
return arc;
|
344
|
+
}
|
345
|
+
}
|
346
|
+
return NULL;
|
347
|
+
}
|
348
|
+
|
349
|
+
twinDestination = destinationNode->twinNode;
|
350
|
+
if (originNode->arcCount <= twinDestination->arcCount) {
|
351
|
+
for (arc = originNode->arc; arc != NULL; arc = arc->next)
|
352
|
+
if (arc->destination == destinationNode)
|
353
|
+
return arc;
|
354
|
+
return NULL;
|
355
|
+
}
|
356
|
+
|
357
|
+
twinOrigin = originNode->twinNode;
|
358
|
+
for (arc = twinDestination->arc; arc != NULL; arc = arc->next)
|
359
|
+
if (arc->destination == twinOrigin)
|
360
|
+
return arc->twinArc;
|
361
|
+
return NULL;
|
362
|
+
}
|
363
|
+
|
364
|
+
void destroyArc(Arc * arc, Graph * graph)
|
365
|
+
{
|
366
|
+
Node *origin, *destination;
|
367
|
+
Arc *twinArc;
|
368
|
+
Arc *currentArc;
|
369
|
+
IDnum lookupIndex;
|
370
|
+
|
371
|
+
if (arc == NULL)
|
372
|
+
return;
|
373
|
+
|
374
|
+
twinArc = arc->twinArc;
|
375
|
+
origin = twinArc->destination->twinNode;
|
376
|
+
destination = arc->destination->twinNode;
|
377
|
+
|
378
|
+
//velvetLog("Destroying arc %p\n", arc);
|
379
|
+
|
380
|
+
// Removing arc from list
|
381
|
+
if (origin->arc == arc) {
|
382
|
+
origin->arc = arc->next;
|
383
|
+
if (origin->arc != NULL)
|
384
|
+
origin->arc->previous = NULL;
|
385
|
+
} else {
|
386
|
+
arc->previous->next = arc->next;
|
387
|
+
if (arc->next != NULL)
|
388
|
+
arc->next->previous = arc->previous;
|
389
|
+
}
|
390
|
+
|
391
|
+
origin->arcCount--;
|
392
|
+
|
393
|
+
if (destination == origin) {
|
394
|
+
if (graph->arcLookupTable != NULL) {
|
395
|
+
lookupIndex =
|
396
|
+
2 * origin->ID - destination->ID +
|
397
|
+
3 * graph->nodeCount;
|
398
|
+
currentArc = graph->arcLookupTable[lookupIndex];
|
399
|
+
if (currentArc == arc)
|
400
|
+
graph->arcLookupTable[lookupIndex] =
|
401
|
+
arc->nextInLookupTable;
|
402
|
+
else {
|
403
|
+
while (currentArc->nextInLookupTable !=
|
404
|
+
arc)
|
405
|
+
currentArc =
|
406
|
+
currentArc->nextInLookupTable;
|
407
|
+
|
408
|
+
currentArc->nextInLookupTable =
|
409
|
+
twinArc->nextInLookupTable;
|
410
|
+
}
|
411
|
+
}
|
412
|
+
|
413
|
+
deallocateArc(arc);
|
414
|
+
return;
|
415
|
+
}
|
416
|
+
// Removing arc's twin from list
|
417
|
+
if (destination->arc == twinArc) {
|
418
|
+
destination->arc = twinArc->next;
|
419
|
+
if (destination->arc != NULL)
|
420
|
+
destination->arc->previous = NULL;
|
421
|
+
} else {
|
422
|
+
twinArc->previous->next = twinArc->next;
|
423
|
+
if (twinArc->next != NULL)
|
424
|
+
twinArc->next->previous = twinArc->previous;
|
425
|
+
}
|
426
|
+
|
427
|
+
destination->arcCount--;
|
428
|
+
|
429
|
+
if (graph->arcLookupTable != NULL) {
|
430
|
+
lookupIndex =
|
431
|
+
2 * origin->ID - destination->ID +
|
432
|
+
3 * graph->nodeCount;
|
433
|
+
currentArc = graph->arcLookupTable[lookupIndex];
|
434
|
+
if (currentArc == arc)
|
435
|
+
graph->arcLookupTable[lookupIndex] =
|
436
|
+
arc->nextInLookupTable;
|
437
|
+
else {
|
438
|
+
while (currentArc->nextInLookupTable != arc)
|
439
|
+
currentArc = currentArc->nextInLookupTable;
|
440
|
+
|
441
|
+
currentArc->nextInLookupTable =
|
442
|
+
arc->nextInLookupTable;
|
443
|
+
}
|
444
|
+
|
445
|
+
lookupIndex =
|
446
|
+
2 * destination->ID - origin->ID +
|
447
|
+
3 * graph->nodeCount;
|
448
|
+
currentArc = graph->arcLookupTable[lookupIndex];
|
449
|
+
if (currentArc == twinArc)
|
450
|
+
graph->arcLookupTable[lookupIndex] =
|
451
|
+
twinArc->nextInLookupTable;
|
452
|
+
else {
|
453
|
+
while (currentArc->nextInLookupTable != twinArc)
|
454
|
+
currentArc = currentArc->nextInLookupTable;
|
455
|
+
|
456
|
+
currentArc->nextInLookupTable =
|
457
|
+
twinArc->nextInLookupTable;
|
458
|
+
}
|
459
|
+
}
|
460
|
+
// Freeing memory
|
461
|
+
deallocateArc(arc);
|
462
|
+
deallocateArc(twinArc);
|
463
|
+
}
|
464
|
+
|
465
|
+
void destroyNode(Node * node, Graph * graph)
|
466
|
+
{
|
467
|
+
Node *twin = node->twinNode;
|
468
|
+
IDnum ID = node->ID;
|
469
|
+
IDnum index;
|
470
|
+
|
471
|
+
//velvetLog("Destroying %d\n and twin %d\n", getNodeID(node), getNodeID(twin));
|
472
|
+
|
473
|
+
if (ID < 0)
|
474
|
+
ID = -ID;
|
475
|
+
|
476
|
+
// Node arcs:
|
477
|
+
while (node->arc != NULL)
|
478
|
+
destroyArc(node->arc, graph);
|
479
|
+
while (twin->arc != NULL)
|
480
|
+
destroyArc(twin->arc, graph);
|
481
|
+
|
482
|
+
// Descriptors
|
483
|
+
free(node->descriptor);
|
484
|
+
free(twin->descriptor);
|
485
|
+
|
486
|
+
// Passage markers
|
487
|
+
while (node->marker != NULL_IDX)
|
488
|
+
destroyPassageMarker(node->marker);
|
489
|
+
|
490
|
+
// Reads starts
|
491
|
+
if (graph->nodeReads != NULL) {
|
492
|
+
index = ID + graph->nodeCount;
|
493
|
+
free(graph->nodeReads[index]);
|
494
|
+
graph->nodeReads[index] = NULL;
|
495
|
+
graph->nodeReadCounts[index] = 0;
|
496
|
+
|
497
|
+
index = -ID + graph->nodeCount;
|
498
|
+
free(graph->nodeReads[index]);
|
499
|
+
graph->nodeReads[index] = NULL;
|
500
|
+
graph->nodeReadCounts[index] = 0;
|
501
|
+
}
|
502
|
+
|
503
|
+
graph->nodes[ID] = NULL;
|
504
|
+
deallocateNode(node);
|
505
|
+
deallocateNode(twin);
|
506
|
+
}
|
507
|
+
|
508
|
+
int outDegree(Node * node)
|
509
|
+
{
|
510
|
+
int result = 0;
|
511
|
+
Arc *arc = node->arc;
|
512
|
+
while (arc != NULL) {
|
513
|
+
result += arc->multiplicity;
|
514
|
+
arc = arc->next;
|
515
|
+
}
|
516
|
+
|
517
|
+
return result;
|
518
|
+
}
|
519
|
+
|
520
|
+
int simpleArcCount(Node * node)
|
521
|
+
{
|
522
|
+
return node->arcCount;
|
523
|
+
}
|
524
|
+
|
525
|
+
int arcCount(Node * node)
|
526
|
+
{
|
527
|
+
int result = 0;
|
528
|
+
Arc *arc;
|
529
|
+
|
530
|
+
if (node == NULL)
|
531
|
+
return result;
|
532
|
+
|
533
|
+
arc = node->arc;
|
534
|
+
while (arc != NULL) {
|
535
|
+
result++;
|
536
|
+
if (arc->destination == node->twinNode)
|
537
|
+
result++;
|
538
|
+
arc = arc->next;
|
539
|
+
}
|
540
|
+
|
541
|
+
return result;
|
542
|
+
|
543
|
+
}
|
544
|
+
|
545
|
+
static Nucleotide getNucleotideInDescriptor(Descriptor * descriptor,
|
546
|
+
Coordinate i)
|
547
|
+
{
|
548
|
+
Descriptor *fourMer = descriptor + i / 4;
|
549
|
+
|
550
|
+
switch (i % 4) {
|
551
|
+
case 0:
|
552
|
+
return (*fourMer & 3);
|
553
|
+
case 1:
|
554
|
+
return (*fourMer & 12) >> 2;
|
555
|
+
case 2:
|
556
|
+
return (*fourMer & 48) >> 4;
|
557
|
+
case 3:
|
558
|
+
return (*fourMer & 192) >> 6;
|
559
|
+
}
|
560
|
+
return 0;
|
561
|
+
}
|
562
|
+
|
563
|
+
Nucleotide getNucleotideInNode(Node * node, Coordinate index) {
|
564
|
+
return getNucleotideInDescriptor(node->descriptor, index);
|
565
|
+
}
|
566
|
+
|
567
|
+
PassageMarkerI getMarker(Node * node)
|
568
|
+
{
|
569
|
+
return node->marker;
|
570
|
+
}
|
571
|
+
|
572
|
+
void setMarker(Node * node, PassageMarkerI marker)
|
573
|
+
{
|
574
|
+
if (node == NULL)
|
575
|
+
return;
|
576
|
+
|
577
|
+
if (marker == NULL_IDX) {
|
578
|
+
node->marker = NULL_IDX;
|
579
|
+
node->twinNode->marker = NULL_IDX;
|
580
|
+
return;
|
581
|
+
}
|
582
|
+
|
583
|
+
node->marker = marker;
|
584
|
+
setTopOfTheNode(marker);
|
585
|
+
node->twinNode->marker = getTwinMarker(marker);
|
586
|
+
setTopOfTheNode(getTwinMarker(marker));
|
587
|
+
}
|
588
|
+
|
589
|
+
void setNodeStatus(Node * node, boolean status)
|
590
|
+
{
|
591
|
+
node->status = status;
|
592
|
+
node->twinNode->status = status;
|
593
|
+
}
|
594
|
+
|
595
|
+
void setSingleNodeStatus(Node * node, boolean status)
|
596
|
+
{
|
597
|
+
node->status = status;
|
598
|
+
}
|
599
|
+
|
600
|
+
boolean getNodeStatus(Node * node)
|
601
|
+
{
|
602
|
+
if (node == NULL)
|
603
|
+
return false;
|
604
|
+
return node->status;
|
605
|
+
}
|
606
|
+
|
607
|
+
IDnum getNodeID(Node * node)
|
608
|
+
{
|
609
|
+
if (node == NULL)
|
610
|
+
return 0;
|
611
|
+
|
612
|
+
return node->ID;
|
613
|
+
}
|
614
|
+
|
615
|
+
void resetNodeStatus(Graph * graph)
|
616
|
+
{
|
617
|
+
IDnum nodeIndex;
|
618
|
+
Node *node;
|
619
|
+
|
620
|
+
for (nodeIndex = 1; nodeIndex <= graph->nodeCount; nodeIndex++) {
|
621
|
+
node = graph->nodes[nodeIndex];
|
622
|
+
if (node == NULL)
|
623
|
+
continue;
|
624
|
+
|
625
|
+
node->status = false;
|
626
|
+
node->twinNode->status = false;
|
627
|
+
}
|
628
|
+
}
|
629
|
+
|
630
|
+
Node *getNodeInGraph(Graph * graph, IDnum nodeID)
|
631
|
+
{
|
632
|
+
if (nodeID == 0)
|
633
|
+
return NULL;
|
634
|
+
else if (nodeID > 0)
|
635
|
+
return graph->nodes[nodeID];
|
636
|
+
else if (graph->nodes[-nodeID] == NULL)
|
637
|
+
return NULL;
|
638
|
+
else
|
639
|
+
return graph->nodes[-nodeID]->twinNode;
|
640
|
+
}
|
641
|
+
|
642
|
+
Arc *getArc(Node * node)
|
643
|
+
{
|
644
|
+
return node->arc;
|
645
|
+
}
|
646
|
+
|
647
|
+
Arc *getNextArc(Arc * arc)
|
648
|
+
{
|
649
|
+
return arc->next;
|
650
|
+
}
|
651
|
+
|
652
|
+
IDnum getMultiplicity(Arc * arc)
|
653
|
+
{
|
654
|
+
if (arc == NULL)
|
655
|
+
return 0;
|
656
|
+
|
657
|
+
return arc->multiplicity;
|
658
|
+
}
|
659
|
+
|
660
|
+
Node *getOrigin(Arc * arc)
|
661
|
+
{
|
662
|
+
if (arc == NULL)
|
663
|
+
return NULL;
|
664
|
+
|
665
|
+
return arc->twinArc->destination->twinNode;
|
666
|
+
}
|
667
|
+
|
668
|
+
Node *getDestination(Arc * arc)
|
669
|
+
{
|
670
|
+
if (arc == NULL)
|
671
|
+
return NULL;
|
672
|
+
|
673
|
+
return arc->destination;
|
674
|
+
}
|
675
|
+
|
676
|
+
IDnum markerCount(Node * node)
|
677
|
+
{
|
678
|
+
IDnum count = 0;
|
679
|
+
PassageMarkerI marker;
|
680
|
+
|
681
|
+
for (marker = getMarker(node); marker != NULL_IDX;
|
682
|
+
marker = getNextInNode(marker))
|
683
|
+
count++;
|
684
|
+
|
685
|
+
return count;
|
686
|
+
}
|
687
|
+
|
688
|
+
void appendNodeSequence(Node * node, TightString * sequence,
|
689
|
+
Coordinate writeIndex)
|
690
|
+
{
|
691
|
+
Coordinate i;
|
692
|
+
Nucleotide nucleotide;
|
693
|
+
|
694
|
+
//velvetLog("Getting sequence from node %d of length %d (%d)\n", getNodeID(node), getNodeLength(node), getLength(nodeLabel));
|
695
|
+
|
696
|
+
for (i = 0; i < getNodeLength(node); i++) {
|
697
|
+
nucleotide =
|
698
|
+
getNucleotideInDescriptor(node->descriptor, i);
|
699
|
+
writeNucleotideAtPosition(nucleotide, i + writeIndex,
|
700
|
+
sequence);
|
701
|
+
}
|
702
|
+
}
|
703
|
+
|
704
|
+
static void writeNucleotideInDescriptor(Nucleotide nucleotide,
|
705
|
+
Descriptor * descriptor,
|
706
|
+
Coordinate i)
|
707
|
+
{
|
708
|
+
Descriptor *fourMer = descriptor + i / 4;
|
709
|
+
switch (i % 4) {
|
710
|
+
case 3:
|
711
|
+
*fourMer &= 63;
|
712
|
+
*fourMer += nucleotide << 6;
|
713
|
+
return;
|
714
|
+
case 2:
|
715
|
+
*fourMer &= 207;
|
716
|
+
*fourMer += nucleotide << 4;
|
717
|
+
return;
|
718
|
+
case 1:
|
719
|
+
*fourMer &= 243;
|
720
|
+
*fourMer += nucleotide << 2;
|
721
|
+
return;
|
722
|
+
case 0:
|
723
|
+
*fourMer &= 252;
|
724
|
+
*fourMer += nucleotide;
|
725
|
+
}
|
726
|
+
}
|
727
|
+
|
728
|
+
static inline Descriptor *mergeDescriptors(Descriptor * descr,
|
729
|
+
Coordinate destinationLength,
|
730
|
+
Descriptor * copy,
|
731
|
+
Coordinate sourceLength,
|
732
|
+
size_t arrayLength)
|
733
|
+
{
|
734
|
+
Descriptor *readPtr, *writePtr;
|
735
|
+
Descriptor readCopy;
|
736
|
+
int readOffset, writeOffset;
|
737
|
+
Descriptor *new = callocOrExit(arrayLength, Descriptor);
|
738
|
+
Coordinate index;
|
739
|
+
|
740
|
+
readPtr = descr;
|
741
|
+
readCopy = *readPtr;
|
742
|
+
writePtr = new;
|
743
|
+
writeOffset = 0;
|
744
|
+
for (index = 0; index < destinationLength; index++) {
|
745
|
+
(*writePtr) >>= 2;
|
746
|
+
(*writePtr) += (readCopy & 3) << 6;
|
747
|
+
readCopy >>= 2;
|
748
|
+
|
749
|
+
writeOffset++;
|
750
|
+
if (writeOffset == 4) {
|
751
|
+
writePtr++;
|
752
|
+
readPtr++;
|
753
|
+
if (index < destinationLength - 1)
|
754
|
+
readCopy = *readPtr;
|
755
|
+
writeOffset = 0;
|
756
|
+
}
|
757
|
+
}
|
758
|
+
|
759
|
+
readPtr = copy;
|
760
|
+
readCopy = *readPtr;
|
761
|
+
readOffset = 0;
|
762
|
+
for (index = 0; index < sourceLength; index++) {
|
763
|
+
(*writePtr) >>= 2;
|
764
|
+
(*writePtr) += (readCopy & 3) << 6;
|
765
|
+
readCopy >>= 2;
|
766
|
+
|
767
|
+
writeOffset++;
|
768
|
+
if (writeOffset == 4) {
|
769
|
+
writePtr++;
|
770
|
+
writeOffset = 0;
|
771
|
+
}
|
772
|
+
|
773
|
+
readOffset++;
|
774
|
+
if (readOffset == 4) {
|
775
|
+
readPtr++;
|
776
|
+
if (index < sourceLength - 1)
|
777
|
+
readCopy = *readPtr;
|
778
|
+
readOffset = 0;
|
779
|
+
}
|
780
|
+
}
|
781
|
+
|
782
|
+
if (writeOffset != 0) {
|
783
|
+
while (writeOffset != 4) {
|
784
|
+
(*writePtr) >>= 2;
|
785
|
+
writeOffset++;
|
786
|
+
}
|
787
|
+
}
|
788
|
+
|
789
|
+
return new;
|
790
|
+
}
|
791
|
+
|
792
|
+
static void addBufferToDescriptor(Node * node, Coordinate length)
|
793
|
+
{
|
794
|
+
Descriptor *descr;
|
795
|
+
Coordinate newLength;
|
796
|
+
size_t arrayLength;
|
797
|
+
Node *twinNode;
|
798
|
+
Coordinate index;
|
799
|
+
Descriptor *old_descriptor;
|
800
|
+
|
801
|
+
if (node == NULL)
|
802
|
+
return;
|
803
|
+
|
804
|
+
twinNode = node->twinNode;
|
805
|
+
descr = node->descriptor;
|
806
|
+
|
807
|
+
// Amendments for empty descriptors
|
808
|
+
if (descr == NULL) {
|
809
|
+
arrayLength = length / 4;
|
810
|
+
if (length % 4 != 0)
|
811
|
+
arrayLength++;
|
812
|
+
|
813
|
+
node->descriptor = callocOrExit(arrayLength, Descriptor);
|
814
|
+
node->length = length;
|
815
|
+
twinNode->descriptor =
|
816
|
+
callocOrExit(arrayLength, Descriptor);
|
817
|
+
twinNode->length = length;
|
818
|
+
return;
|
819
|
+
}
|
820
|
+
|
821
|
+
newLength = node->length + length;
|
822
|
+
arrayLength = newLength / 4;
|
823
|
+
if (newLength % 4 != 0)
|
824
|
+
arrayLength++;
|
825
|
+
|
826
|
+
// Merging forward descriptors
|
827
|
+
node->descriptor =
|
828
|
+
reallocOrExit(node->descriptor, arrayLength, Descriptor);
|
829
|
+
|
830
|
+
for (index = node->length; index < newLength; index++)
|
831
|
+
writeNucleotideInDescriptor(ADENINE, node->descriptor,
|
832
|
+
index);
|
833
|
+
node->length = newLength;
|
834
|
+
|
835
|
+
// Merging reverse descriptors
|
836
|
+
old_descriptor = twinNode->descriptor;
|
837
|
+
twinNode->descriptor = callocOrExit(arrayLength, Descriptor);
|
838
|
+
for (index = 0; index < twinNode->length; index++)
|
839
|
+
writeNucleotideInDescriptor(getNucleotideInDescriptor
|
840
|
+
(old_descriptor, index),
|
841
|
+
twinNode->descriptor,
|
842
|
+
index + length);
|
843
|
+
for (index = 0; index < length; index++)
|
844
|
+
writeNucleotideInDescriptor(THYMINE, twinNode->descriptor,
|
845
|
+
index);
|
846
|
+
free(old_descriptor);
|
847
|
+
twinNode->length = newLength;
|
848
|
+
}
|
849
|
+
|
850
|
+
void appendDescriptors(Node * destination, Node * source)
|
851
|
+
{
|
852
|
+
Descriptor *copy;
|
853
|
+
Descriptor *twinCopy;
|
854
|
+
Descriptor *descr;
|
855
|
+
Descriptor *twinDescr;
|
856
|
+
Coordinate newLength, destinationLength, sourceLength;
|
857
|
+
size_t arrayLength;
|
858
|
+
Descriptor *new;
|
859
|
+
Node *twinDestination;
|
860
|
+
|
861
|
+
if (source == NULL || destination == NULL)
|
862
|
+
return;
|
863
|
+
|
864
|
+
twinDestination = destination->twinNode;
|
865
|
+
descr = destination->descriptor;
|
866
|
+
twinDescr = twinDestination->descriptor;
|
867
|
+
copy = source->descriptor;
|
868
|
+
twinCopy = source->twinNode->descriptor;
|
869
|
+
|
870
|
+
// Amendments for empty descriptors
|
871
|
+
if (getNodeLength(source) == 0)
|
872
|
+
return;
|
873
|
+
if (getNodeLength(destination) == 0) {
|
874
|
+
destination->descriptor = copy;
|
875
|
+
twinDestination->descriptor = twinCopy;
|
876
|
+
source->descriptor = NULL;
|
877
|
+
source->twinNode->descriptor = NULL;
|
878
|
+
destination->length = source->length;
|
879
|
+
destination->twinNode->length = source->length;
|
880
|
+
source->length = 0;
|
881
|
+
source->twinNode->length = 0;
|
882
|
+
return;
|
883
|
+
}
|
884
|
+
|
885
|
+
destinationLength = destination->length;
|
886
|
+
sourceLength = source->length;
|
887
|
+
newLength = destinationLength + sourceLength;
|
888
|
+
arrayLength = newLength / 4;
|
889
|
+
if (newLength % 4 != 0)
|
890
|
+
arrayLength++;
|
891
|
+
|
892
|
+
// Merging forward descriptors
|
893
|
+
new =
|
894
|
+
mergeDescriptors(descr, destinationLength, copy, sourceLength,
|
895
|
+
arrayLength);
|
896
|
+
free(descr);
|
897
|
+
destination->descriptor = new;
|
898
|
+
destination->length = newLength;
|
899
|
+
|
900
|
+
// Merging reverse descriptors
|
901
|
+
new =
|
902
|
+
mergeDescriptors(twinCopy, sourceLength, twinDescr,
|
903
|
+
destinationLength, arrayLength);
|
904
|
+
free(twinDescr);
|
905
|
+
twinDestination->descriptor = new;
|
906
|
+
twinDestination->length = newLength;
|
907
|
+
}
|
908
|
+
|
909
|
+
static void catDescriptors(Descriptor * descr, Coordinate destinationLength, Descriptor * copy, Coordinate sourceLength)
|
910
|
+
{
|
911
|
+
Coordinate index;
|
912
|
+
Nucleotide nucleotide;
|
913
|
+
|
914
|
+
for (index = 0; index < sourceLength; index++) {
|
915
|
+
nucleotide = getNucleotideInDescriptor(copy, index);
|
916
|
+
writeNucleotideInDescriptor(nucleotide, descr, index + destinationLength);
|
917
|
+
}
|
918
|
+
}
|
919
|
+
|
920
|
+
static void reverseCatDescriptors(Descriptor * descr, Coordinate destinationLength, Descriptor * copy, Coordinate sourceLength, Coordinate totalLength)
|
921
|
+
{
|
922
|
+
Coordinate shift = totalLength - destinationLength - sourceLength;
|
923
|
+
Coordinate index;
|
924
|
+
Nucleotide nucleotide;
|
925
|
+
|
926
|
+
for (index = 0; index < sourceLength; index++) {
|
927
|
+
nucleotide = getNucleotideInDescriptor(copy, index);
|
928
|
+
writeNucleotideInDescriptor(nucleotide, descr, index + shift);
|
929
|
+
}
|
930
|
+
}
|
931
|
+
|
932
|
+
void directlyAppendDescriptors(Node * destination, Node * source, Coordinate totalLength)
|
933
|
+
{
|
934
|
+
Descriptor *copy;
|
935
|
+
Descriptor *twinCopy;
|
936
|
+
Descriptor *descr;
|
937
|
+
Descriptor *twinDescr;
|
938
|
+
Coordinate destinationLength, sourceLength;
|
939
|
+
|
940
|
+
if (source == NULL || destination == NULL)
|
941
|
+
return;
|
942
|
+
|
943
|
+
descr = destination->descriptor;
|
944
|
+
twinDescr = destination->twinNode->descriptor;
|
945
|
+
copy = source->descriptor;
|
946
|
+
twinCopy = source->twinNode->descriptor;
|
947
|
+
|
948
|
+
// Amendments for empty descriptors
|
949
|
+
if (getNodeLength(source) == 0)
|
950
|
+
return;
|
951
|
+
|
952
|
+
destinationLength = destination->length;
|
953
|
+
sourceLength = source->length;
|
954
|
+
|
955
|
+
// Merging forward descriptors
|
956
|
+
catDescriptors(descr, destinationLength, copy, sourceLength);
|
957
|
+
|
958
|
+
// Merging reverse descriptors
|
959
|
+
reverseCatDescriptors(twinDescr, destinationLength, twinCopy, sourceLength, totalLength);
|
960
|
+
|
961
|
+
destination->length += source->length;
|
962
|
+
destination->twinNode->length += source->length;
|
963
|
+
}
|
964
|
+
|
965
|
+
static void copyDownDescriptor(Descriptor ** writePtr, int *writeOffset,
|
966
|
+
Descriptor * source, Coordinate length)
|
967
|
+
{
|
968
|
+
Descriptor *readPtr = source;
|
969
|
+
Descriptor readCopy = *readPtr;
|
970
|
+
int readOffset = 0;
|
971
|
+
Coordinate index;
|
972
|
+
|
973
|
+
for (index = 0; index < length; index++) {
|
974
|
+
(**writePtr) >>= 2;
|
975
|
+
(**writePtr) += (readCopy & 3) << 6;
|
976
|
+
readCopy >>= 2;
|
977
|
+
|
978
|
+
(*writeOffset)++;
|
979
|
+
if (*writeOffset == 4) {
|
980
|
+
(*writePtr)++;
|
981
|
+
*writeOffset = 0;
|
982
|
+
}
|
983
|
+
|
984
|
+
readOffset++;
|
985
|
+
if (readOffset == 4) {
|
986
|
+
readPtr++;
|
987
|
+
if (index < length - 1)
|
988
|
+
readCopy = *readPtr;
|
989
|
+
readOffset = 0;
|
990
|
+
}
|
991
|
+
}
|
992
|
+
}
|
993
|
+
|
994
|
+
static void copyDownSequence(Descriptor ** writePtr, int *writeOffset,
|
995
|
+
TightString * sequence, Coordinate start,
|
996
|
+
Coordinate finish, int WORDLENGTH)
|
997
|
+
{
|
998
|
+
boolean forward = (start < finish);
|
999
|
+
Coordinate sourceLength = finish - start;
|
1000
|
+
Coordinate index;
|
1001
|
+
Nucleotide nucleotide;
|
1002
|
+
|
1003
|
+
if (!forward)
|
1004
|
+
sourceLength *= -1;
|
1005
|
+
|
1006
|
+
for (index = 0; index < sourceLength; index++) {
|
1007
|
+
if (forward)
|
1008
|
+
nucleotide =
|
1009
|
+
getNucleotide(start + WORDLENGTH - 1 + index,
|
1010
|
+
sequence);
|
1011
|
+
else
|
1012
|
+
nucleotide =
|
1013
|
+
#ifndef COLOR
|
1014
|
+
3 - getNucleotide(start - index - 1, sequence);
|
1015
|
+
#else
|
1016
|
+
getNucleotide(start - index - 1, sequence);
|
1017
|
+
#endif
|
1018
|
+
|
1019
|
+
(**writePtr) >>= 2;
|
1020
|
+
(**writePtr) += nucleotide << 6;
|
1021
|
+
|
1022
|
+
(*writeOffset)++;
|
1023
|
+
if (*writeOffset == 4) {
|
1024
|
+
(*writePtr)++;
|
1025
|
+
*writeOffset = 0;
|
1026
|
+
}
|
1027
|
+
}
|
1028
|
+
}
|
1029
|
+
|
1030
|
+
static Descriptor *appendSequenceToDescriptor(Descriptor * descr,
|
1031
|
+
Coordinate nodeLength,
|
1032
|
+
PassageMarkerI marker,
|
1033
|
+
TightString *sequences,
|
1034
|
+
int WORDLENGTH,
|
1035
|
+
size_t arrayLength,
|
1036
|
+
boolean downStream)
|
1037
|
+
{
|
1038
|
+
int writeOffset = 0;
|
1039
|
+
Descriptor *new = callocOrExit(arrayLength, Descriptor);
|
1040
|
+
Descriptor *writePtr = new;
|
1041
|
+
TightString *sequence;
|
1042
|
+
IDnum sequenceID = getPassageMarkerSequenceID(marker);
|
1043
|
+
Coordinate start = getPassageMarkerStart(marker);
|
1044
|
+
Coordinate finish = getPassageMarkerFinish(marker);
|
1045
|
+
|
1046
|
+
if (sequenceID > 0)
|
1047
|
+
sequence = getTightStringInArray(sequences, sequenceID - 1);
|
1048
|
+
else
|
1049
|
+
sequence = getTightStringInArray(sequences, -sequenceID - 1);
|
1050
|
+
|
1051
|
+
if (downStream)
|
1052
|
+
copyDownDescriptor(&writePtr, &writeOffset, descr,
|
1053
|
+
nodeLength);
|
1054
|
+
|
1055
|
+
copyDownSequence(&writePtr, &writeOffset, sequence, start, finish,
|
1056
|
+
WORDLENGTH);
|
1057
|
+
|
1058
|
+
if (!downStream)
|
1059
|
+
copyDownDescriptor(&writePtr, &writeOffset, descr,
|
1060
|
+
nodeLength);
|
1061
|
+
|
1062
|
+
if (writeOffset != 0) {
|
1063
|
+
while (writeOffset != 4) {
|
1064
|
+
(*writePtr) >>= 2;
|
1065
|
+
writeOffset++;
|
1066
|
+
}
|
1067
|
+
}
|
1068
|
+
|
1069
|
+
return new;
|
1070
|
+
}
|
1071
|
+
|
1072
|
+
void appendSequence(Node * node, TightString * reads,
|
1073
|
+
PassageMarkerI guide, Graph * graph)
|
1074
|
+
{
|
1075
|
+
Descriptor *descr;
|
1076
|
+
Descriptor *twinDescr;
|
1077
|
+
Coordinate newLength, nodeLength, sourceLength;
|
1078
|
+
size_t arrayLength;
|
1079
|
+
Descriptor *new;
|
1080
|
+
Node *twinNode;
|
1081
|
+
|
1082
|
+
if (node == NULL)
|
1083
|
+
return;
|
1084
|
+
|
1085
|
+
twinNode = node->twinNode;
|
1086
|
+
descr = node->descriptor;
|
1087
|
+
twinDescr = twinNode->descriptor;
|
1088
|
+
nodeLength = node->length;
|
1089
|
+
sourceLength = getPassageMarkerLength(guide);
|
1090
|
+
|
1091
|
+
// Amendments for empty descriptors
|
1092
|
+
if (sourceLength == 0)
|
1093
|
+
return;
|
1094
|
+
|
1095
|
+
newLength = nodeLength + sourceLength;
|
1096
|
+
arrayLength = newLength / 4;
|
1097
|
+
if (newLength % 4 != 0)
|
1098
|
+
arrayLength++;
|
1099
|
+
|
1100
|
+
// Merging forward descriptors
|
1101
|
+
new =
|
1102
|
+
appendSequenceToDescriptor(descr, nodeLength, guide, reads,
|
1103
|
+
getWordLength(graph), arrayLength,
|
1104
|
+
true);
|
1105
|
+
free(descr);
|
1106
|
+
node->descriptor = new;
|
1107
|
+
node->length = newLength;
|
1108
|
+
|
1109
|
+
// Merging reverse descriptors
|
1110
|
+
new =
|
1111
|
+
appendSequenceToDescriptor(twinDescr, nodeLength,
|
1112
|
+
getTwinMarker(guide), reads,
|
1113
|
+
getWordLength(graph), arrayLength,
|
1114
|
+
false);
|
1115
|
+
free(twinDescr);
|
1116
|
+
twinNode->descriptor = new;
|
1117
|
+
twinNode->length = newLength;
|
1118
|
+
}
|
1119
|
+
|
1120
|
+
void setMultiplicity(Arc * arc, IDnum mult)
|
1121
|
+
{
|
1122
|
+
arc->multiplicity = mult;
|
1123
|
+
arc->twinArc->multiplicity = mult;
|
1124
|
+
}
|
1125
|
+
|
1126
|
+
// Reshuffles the graph->nodes array to remove NULL pointers
|
1127
|
+
// Beware that node IDs are accordingly reshuffled (all pointers remain valid though)
|
1128
|
+
void renumberNodes(Graph * graph)
|
1129
|
+
{
|
1130
|
+
IDnum nodeIndex;
|
1131
|
+
Node *currentNode;
|
1132
|
+
IDnum counter = 0;
|
1133
|
+
IDnum nodes = graph->nodeCount;
|
1134
|
+
IDnum newIndex;
|
1135
|
+
|
1136
|
+
velvetLog("Renumbering nodes\n");
|
1137
|
+
velvetLog("Initial node count %li\n", (long) graph->nodeCount);
|
1138
|
+
|
1139
|
+
for (nodeIndex = 1; nodeIndex <= nodes; nodeIndex++) {
|
1140
|
+
currentNode = getNodeInGraph(graph, nodeIndex);
|
1141
|
+
|
1142
|
+
if (currentNode == NULL)
|
1143
|
+
counter++;
|
1144
|
+
else if (counter != 0) {
|
1145
|
+
newIndex = nodeIndex - counter;
|
1146
|
+
currentNode->ID = newIndex;
|
1147
|
+
currentNode->twinNode->ID = -newIndex;
|
1148
|
+
graph->nodes[newIndex] = currentNode;
|
1149
|
+
|
1150
|
+
if (graph->nodeReads != NULL) {
|
1151
|
+
graph->nodeReads[newIndex + nodes] =
|
1152
|
+
graph->nodeReads[nodeIndex + nodes];
|
1153
|
+
graph->nodeReadCounts[newIndex + nodes] =
|
1154
|
+
graph->nodeReadCounts[nodeIndex +
|
1155
|
+
nodes];
|
1156
|
+
|
1157
|
+
graph->nodeReads[nodeIndex + nodes] = NULL;
|
1158
|
+
graph->nodeReadCounts[nodeIndex + nodes] =
|
1159
|
+
0;
|
1160
|
+
|
1161
|
+
graph->nodeReads[-newIndex + nodes] =
|
1162
|
+
graph->nodeReads[-nodeIndex + nodes];
|
1163
|
+
graph->nodeReadCounts[-newIndex + nodes] =
|
1164
|
+
graph->nodeReadCounts[-nodeIndex +
|
1165
|
+
nodes];
|
1166
|
+
|
1167
|
+
graph->nodeReads[-nodeIndex + nodes] =
|
1168
|
+
NULL;
|
1169
|
+
graph->nodeReadCounts[-nodeIndex + nodes] =
|
1170
|
+
0;
|
1171
|
+
}
|
1172
|
+
|
1173
|
+
if (graph->gapMarkers != NULL) {
|
1174
|
+
graph->gapMarkers[newIndex] =
|
1175
|
+
graph->gapMarkers[nodeIndex];
|
1176
|
+
graph->gapMarkers[nodeIndex] = NULL;
|
1177
|
+
}
|
1178
|
+
}
|
1179
|
+
}
|
1180
|
+
|
1181
|
+
// Shitfting array to the left
|
1182
|
+
if (graph->nodeReads != NULL && counter != 0) {
|
1183
|
+
for (nodeIndex = counter; nodeIndex <= 2 * nodes - counter;
|
1184
|
+
nodeIndex++) {
|
1185
|
+
graph->nodeReads[nodeIndex - counter] =
|
1186
|
+
graph->nodeReads[nodeIndex];
|
1187
|
+
graph->nodeReadCounts[nodeIndex - counter] =
|
1188
|
+
graph->nodeReadCounts[nodeIndex];
|
1189
|
+
}
|
1190
|
+
}
|
1191
|
+
|
1192
|
+
// Rellocating node space
|
1193
|
+
graph->nodeCount -= counter;
|
1194
|
+
graph->nodes =
|
1195
|
+
reallocOrExit(graph->nodes, graph->nodeCount + 1, Node *);
|
1196
|
+
|
1197
|
+
// Reallocating short read marker arrays
|
1198
|
+
if (graph->nodeReads != NULL) {
|
1199
|
+
graph->nodeReads =
|
1200
|
+
reallocOrExit(graph->nodeReads,
|
1201
|
+
2 * graph->nodeCount +
|
1202
|
+
1, ShortReadMarker *);
|
1203
|
+
graph->nodeReadCounts =
|
1204
|
+
reallocOrExit(graph->nodeReadCounts,
|
1205
|
+
2 * graph->nodeCount + 1, IDnum);
|
1206
|
+
}
|
1207
|
+
|
1208
|
+
// Reallocating gap marker table
|
1209
|
+
if (graph->gapMarkers != NULL)
|
1210
|
+
graph->gapMarkers = reallocOrExit(graph->gapMarkers,
|
1211
|
+
graph->nodeCount +
|
1212
|
+
1, GapMarker *);
|
1213
|
+
|
1214
|
+
velvetLog("Removed %li null nodes\n", (long) counter);
|
1215
|
+
}
|
1216
|
+
|
1217
|
+
void splitNodeDescriptor(Node * source, Node * target, Coordinate offset)
|
1218
|
+
{
|
1219
|
+
Coordinate originalLength = source->length;
|
1220
|
+
Coordinate backLength = originalLength - offset;
|
1221
|
+
Coordinate index;
|
1222
|
+
Descriptor *descriptor, *new;
|
1223
|
+
size_t arrayLength;
|
1224
|
+
Nucleotide nucleotide;
|
1225
|
+
|
1226
|
+
source->length = offset;
|
1227
|
+
source->twinNode->length = offset;
|
1228
|
+
|
1229
|
+
if (target != NULL) {
|
1230
|
+
target->length = backLength;
|
1231
|
+
target->twinNode->length = backLength;
|
1232
|
+
free(target->descriptor);
|
1233
|
+
free(target->twinNode->descriptor);
|
1234
|
+
target->descriptor = NULL;
|
1235
|
+
target->twinNode->descriptor = NULL;
|
1236
|
+
}
|
1237
|
+
|
1238
|
+
if (backLength == 0)
|
1239
|
+
return;
|
1240
|
+
|
1241
|
+
descriptor = source->descriptor;
|
1242
|
+
|
1243
|
+
arrayLength = backLength / 4;
|
1244
|
+
if (backLength % 4 > 0)
|
1245
|
+
arrayLength++;
|
1246
|
+
|
1247
|
+
if (target != NULL) {
|
1248
|
+
// Target node .. forwards
|
1249
|
+
new = mallocOrExit(arrayLength, Descriptor);
|
1250
|
+
target->descriptor = new;
|
1251
|
+
for (index = 0; index < backLength; index++) {
|
1252
|
+
nucleotide =
|
1253
|
+
getNucleotideInDescriptor(descriptor, index);
|
1254
|
+
writeNucleotideInDescriptor(nucleotide, new,
|
1255
|
+
index);
|
1256
|
+
}
|
1257
|
+
}
|
1258
|
+
// Source node
|
1259
|
+
for (index = backLength; index < originalLength; index++) {
|
1260
|
+
nucleotide = getNucleotideInDescriptor(descriptor, index);
|
1261
|
+
writeNucleotideInDescriptor(nucleotide, descriptor,
|
1262
|
+
index - backLength);
|
1263
|
+
}
|
1264
|
+
|
1265
|
+
if (target == NULL)
|
1266
|
+
return;
|
1267
|
+
|
1268
|
+
// target node other way
|
1269
|
+
descriptor = source->twinNode->descriptor;
|
1270
|
+
new = mallocOrExit(arrayLength, Descriptor);
|
1271
|
+
target->twinNode->descriptor = new;
|
1272
|
+
|
1273
|
+
for (index = offset; index < originalLength; index++) {
|
1274
|
+
nucleotide = getNucleotideInDescriptor(descriptor, index);
|
1275
|
+
writeNucleotideInDescriptor(nucleotide, new,
|
1276
|
+
index - offset);
|
1277
|
+
}
|
1278
|
+
}
|
1279
|
+
|
1280
|
+
void reduceNode(Node * node)
|
1281
|
+
{
|
1282
|
+
free(node->descriptor);
|
1283
|
+
node->descriptor = NULL;
|
1284
|
+
node->length = 0;
|
1285
|
+
|
1286
|
+
free(node->twinNode->descriptor);
|
1287
|
+
node->twinNode->descriptor = NULL;
|
1288
|
+
node->twinNode->length = 0;
|
1289
|
+
}
|
1290
|
+
|
1291
|
+
// Allocate memory for an empty graph created with sequenceCount different sequences
|
1292
|
+
Graph *emptyGraph(IDnum sequenceCount, int wordLength)
|
1293
|
+
{
|
1294
|
+
Graph *newGraph = mallocOrExit(1, Graph);
|
1295
|
+
newGraph->sequenceCount = sequenceCount;
|
1296
|
+
newGraph->arcLookupTable = NULL;
|
1297
|
+
newGraph->nodeReads = NULL;
|
1298
|
+
newGraph->nodeReadCounts = NULL;
|
1299
|
+
newGraph->wordLength = wordLength;
|
1300
|
+
newGraph->gapMarkers = NULL;
|
1301
|
+
return newGraph;
|
1302
|
+
}
|
1303
|
+
|
1304
|
+
static Descriptor *newPositiveDescriptor(IDnum sequenceID,
|
1305
|
+
Coordinate start,
|
1306
|
+
Coordinate finish,
|
1307
|
+
TightString *sequences,
|
1308
|
+
int WORDLENGTH)
|
1309
|
+
{
|
1310
|
+
Coordinate index;
|
1311
|
+
Nucleotide nucleotide;
|
1312
|
+
TightString *tString = getTightStringInArray (sequences, sequenceID - 1);
|
1313
|
+
Coordinate length = finish - start;
|
1314
|
+
Descriptor *res;
|
1315
|
+
size_t arrayLength = length / 4;
|
1316
|
+
|
1317
|
+
if (length % 4 > 0)
|
1318
|
+
arrayLength++;
|
1319
|
+
|
1320
|
+
res = mallocOrExit(arrayLength, Descriptor);
|
1321
|
+
|
1322
|
+
for (index = 0; index < length; index++) {
|
1323
|
+
nucleotide =
|
1324
|
+
getNucleotide(start + index + WORDLENGTH - 1, tString);
|
1325
|
+
writeNucleotideInDescriptor(nucleotide, res, index);
|
1326
|
+
}
|
1327
|
+
|
1328
|
+
return res;
|
1329
|
+
|
1330
|
+
}
|
1331
|
+
|
1332
|
+
static Descriptor *newNegativeDescriptor(IDnum sequenceID,
|
1333
|
+
Coordinate start,
|
1334
|
+
Coordinate finish,
|
1335
|
+
TightString *sequences,
|
1336
|
+
int WORDLENGTH)
|
1337
|
+
{
|
1338
|
+
Coordinate index;
|
1339
|
+
Nucleotide nucleotide;
|
1340
|
+
TightString *tString = getTightStringInArray (sequences, -sequenceID - 1);
|
1341
|
+
Coordinate length = start - finish;
|
1342
|
+
Descriptor *res;
|
1343
|
+
size_t arrayLength = length / 4;
|
1344
|
+
|
1345
|
+
if (length % 4 > 0)
|
1346
|
+
arrayLength++;
|
1347
|
+
|
1348
|
+
res = mallocOrExit(arrayLength, Descriptor);
|
1349
|
+
|
1350
|
+
for (index = 0; index < length; index++) {
|
1351
|
+
nucleotide = getNucleotide(start - index, tString);
|
1352
|
+
#ifndef COLOR
|
1353
|
+
writeNucleotideInDescriptor(3 - nucleotide, res, index);
|
1354
|
+
#else
|
1355
|
+
writeNucleotideInDescriptor(nucleotide, res, index);
|
1356
|
+
#endif
|
1357
|
+
}
|
1358
|
+
|
1359
|
+
return res;
|
1360
|
+
|
1361
|
+
}
|
1362
|
+
|
1363
|
+
static Descriptor *newDescriptor(IDnum sequenceID, Coordinate start,
|
1364
|
+
Coordinate finish,
|
1365
|
+
TightString * sequences, int WORDLENGTH)
|
1366
|
+
{
|
1367
|
+
if (sequenceID > 0)
|
1368
|
+
return newPositiveDescriptor(sequenceID, start, finish,
|
1369
|
+
sequences, WORDLENGTH);
|
1370
|
+
else
|
1371
|
+
return newNegativeDescriptor(sequenceID, start, finish,
|
1372
|
+
sequences, WORDLENGTH);
|
1373
|
+
}
|
1374
|
+
|
1375
|
+
// Constructor
|
1376
|
+
// Memory allocated
|
1377
|
+
Node *newNode(IDnum sequenceID, Coordinate start, Coordinate finish,
|
1378
|
+
Coordinate offset, IDnum ID, TightString * sequences,
|
1379
|
+
int WORDLENGTH)
|
1380
|
+
{
|
1381
|
+
Node *newnd = allocateNode();
|
1382
|
+
Node *antiNode = allocateNode();
|
1383
|
+
|
1384
|
+
newnd->ID = ID;
|
1385
|
+
newnd->descriptor =
|
1386
|
+
newDescriptor(sequenceID, start + offset, finish + offset,
|
1387
|
+
sequences, WORDLENGTH);
|
1388
|
+
newnd->arc = NULL;
|
1389
|
+
newnd->arcCount = 0;
|
1390
|
+
newnd->marker = NULL_IDX;
|
1391
|
+
newnd->status = false;
|
1392
|
+
|
1393
|
+
#ifndef SINGLE_COV_CAT
|
1394
|
+
Category cat;
|
1395
|
+
for (cat = 0; cat < CATEGORIES; cat++) {
|
1396
|
+
newnd->virtualCoverage[cat] = 0;
|
1397
|
+
newnd->originalVirtualCoverage[cat] = 0;
|
1398
|
+
}
|
1399
|
+
#else
|
1400
|
+
newnd->virtualCoverage = 0;
|
1401
|
+
#endif
|
1402
|
+
|
1403
|
+
antiNode->ID = -ID;
|
1404
|
+
antiNode->descriptor =
|
1405
|
+
newDescriptor(-sequenceID, finish + offset - 1,
|
1406
|
+
start + offset - 1, sequences, WORDLENGTH);
|
1407
|
+
antiNode->arc = NULL;
|
1408
|
+
antiNode->arcCount = 0;
|
1409
|
+
antiNode->marker = NULL_IDX;
|
1410
|
+
antiNode->status = false;
|
1411
|
+
|
1412
|
+
#ifndef SINGLE_COV_CAT
|
1413
|
+
for (cat = 0; cat < CATEGORIES; cat++) {
|
1414
|
+
antiNode->virtualCoverage[cat] = 0;
|
1415
|
+
antiNode->originalVirtualCoverage[cat] = 0;
|
1416
|
+
}
|
1417
|
+
#else
|
1418
|
+
antiNode->virtualCoverage = 0;
|
1419
|
+
#endif
|
1420
|
+
|
1421
|
+
newnd->twinNode = antiNode;
|
1422
|
+
antiNode->twinNode = newnd;
|
1423
|
+
|
1424
|
+
if (sequenceID > 0) {
|
1425
|
+
newnd->length = finish - start;
|
1426
|
+
antiNode->length = finish - start;
|
1427
|
+
} else {
|
1428
|
+
newnd->length = start - finish;
|
1429
|
+
antiNode->length = start - finish;
|
1430
|
+
}
|
1431
|
+
|
1432
|
+
return newnd;
|
1433
|
+
}
|
1434
|
+
|
1435
|
+
void allocateNodeSpace(Graph * graph, IDnum nodeCount)
|
1436
|
+
{
|
1437
|
+
graph->nodes = callocOrExit(nodeCount + 1, Node *);
|
1438
|
+
graph->nodeCount = nodeCount;
|
1439
|
+
}
|
1440
|
+
|
1441
|
+
boolean getUniqueness(Node * node)
|
1442
|
+
{
|
1443
|
+
return node->uniqueness;
|
1444
|
+
}
|
1445
|
+
|
1446
|
+
void setUniqueness(Node * node, boolean value)
|
1447
|
+
{
|
1448
|
+
node->uniqueness = value;
|
1449
|
+
node->twinNode->uniqueness = value;
|
1450
|
+
}
|
1451
|
+
|
1452
|
+
Node *emptyNode()
|
1453
|
+
{
|
1454
|
+
Node *newnd = allocateNode();
|
1455
|
+
Node *antiNode = allocateNode();
|
1456
|
+
|
1457
|
+
newnd->ID = 0;
|
1458
|
+
newnd->descriptor = NULL;
|
1459
|
+
newnd->arc = NULL;
|
1460
|
+
newnd->arcCount = 0;
|
1461
|
+
newnd->marker = NULL_IDX;
|
1462
|
+
newnd->length = 0;
|
1463
|
+
newnd->uniqueness = false;
|
1464
|
+
|
1465
|
+
#ifndef SINGLE_COV_CAT
|
1466
|
+
Category cat;
|
1467
|
+
for (cat = 0; cat < CATEGORIES; cat++) {
|
1468
|
+
newnd->virtualCoverage[cat] = 0;
|
1469
|
+
newnd->originalVirtualCoverage[cat] = 0;
|
1470
|
+
}
|
1471
|
+
#else
|
1472
|
+
newnd->virtualCoverage = 0;
|
1473
|
+
#endif
|
1474
|
+
|
1475
|
+
antiNode->ID = 0;
|
1476
|
+
antiNode->descriptor = NULL;
|
1477
|
+
antiNode->arc = NULL;
|
1478
|
+
antiNode->arcCount = 0;
|
1479
|
+
antiNode->marker = NULL_IDX;
|
1480
|
+
antiNode->length = 0;
|
1481
|
+
antiNode->uniqueness = false;
|
1482
|
+
|
1483
|
+
#ifndef SINGLE_COV_CAT
|
1484
|
+
for (cat = 0; cat < CATEGORIES; cat++) {
|
1485
|
+
antiNode->virtualCoverage[cat] = 0;
|
1486
|
+
antiNode->originalVirtualCoverage[cat] = 0;
|
1487
|
+
}
|
1488
|
+
#else
|
1489
|
+
antiNode->virtualCoverage = 0;
|
1490
|
+
#endif
|
1491
|
+
|
1492
|
+
newnd->twinNode = antiNode;
|
1493
|
+
antiNode->twinNode = newnd;
|
1494
|
+
|
1495
|
+
return newnd;
|
1496
|
+
|
1497
|
+
}
|
1498
|
+
|
1499
|
+
Node *addEmptyNodeToGraph(Graph * graph, IDnum ID)
|
1500
|
+
{
|
1501
|
+
Node *newnd = emptyNode();
|
1502
|
+
|
1503
|
+
newnd->ID = ID;
|
1504
|
+
newnd->twinNode->ID = -ID;
|
1505
|
+
|
1506
|
+
graph->nodes[ID] = newnd;
|
1507
|
+
|
1508
|
+
return newnd;
|
1509
|
+
|
1510
|
+
}
|
1511
|
+
|
1512
|
+
#ifndef SINGLE_COV_CAT
|
1513
|
+
|
1514
|
+
void setVirtualCoverage(Node * node, Category category,
|
1515
|
+
Coordinate coverage)
|
1516
|
+
{
|
1517
|
+
node->virtualCoverage[category] = coverage;
|
1518
|
+
node->twinNode->virtualCoverage[category] =
|
1519
|
+
node->virtualCoverage[category];
|
1520
|
+
}
|
1521
|
+
|
1522
|
+
void incrementVirtualCoverage(Node * node, Category category,
|
1523
|
+
Coordinate coverage)
|
1524
|
+
{
|
1525
|
+
node->virtualCoverage[category] += coverage;
|
1526
|
+
node->twinNode->virtualCoverage[category] =
|
1527
|
+
node->virtualCoverage[category];
|
1528
|
+
}
|
1529
|
+
|
1530
|
+
Coordinate getVirtualCoverage(Node * node, Category category)
|
1531
|
+
{
|
1532
|
+
return node->virtualCoverage[category];
|
1533
|
+
}
|
1534
|
+
|
1535
|
+
Coordinate getTotalCoverage(Node * node)
|
1536
|
+
{
|
1537
|
+
Category cat;
|
1538
|
+
Coordinate coverage = 0;
|
1539
|
+
|
1540
|
+
for (cat = 0; cat < CATEGORIES; cat++)
|
1541
|
+
coverage += node->virtualCoverage[cat];
|
1542
|
+
|
1543
|
+
return coverage;
|
1544
|
+
}
|
1545
|
+
|
1546
|
+
void setOriginalVirtualCoverage(Node * node, Category category,
|
1547
|
+
Coordinate coverage)
|
1548
|
+
{
|
1549
|
+
node->originalVirtualCoverage[category] = coverage;
|
1550
|
+
node->twinNode->originalVirtualCoverage[category] =
|
1551
|
+
node->originalVirtualCoverage[category];
|
1552
|
+
}
|
1553
|
+
|
1554
|
+
void incrementOriginalVirtualCoverage(Node * node, Category category,
|
1555
|
+
Coordinate coverage)
|
1556
|
+
{
|
1557
|
+
node->originalVirtualCoverage[category] += coverage;
|
1558
|
+
node->twinNode->originalVirtualCoverage[category] =
|
1559
|
+
node->originalVirtualCoverage[category];
|
1560
|
+
}
|
1561
|
+
|
1562
|
+
Coordinate getOriginalVirtualCoverage(Node * node, Category category)
|
1563
|
+
{
|
1564
|
+
return node->originalVirtualCoverage[category];
|
1565
|
+
}
|
1566
|
+
|
1567
|
+
#else
|
1568
|
+
|
1569
|
+
void setVirtualCoverage(Node * node,
|
1570
|
+
Coordinate coverage)
|
1571
|
+
{
|
1572
|
+
node->virtualCoverage = coverage;
|
1573
|
+
node->twinNode->virtualCoverage = coverage;
|
1574
|
+
}
|
1575
|
+
|
1576
|
+
void incrementVirtualCoverage(Node * node,
|
1577
|
+
Coordinate coverage)
|
1578
|
+
{
|
1579
|
+
node->virtualCoverage += coverage;
|
1580
|
+
node->twinNode->virtualCoverage += coverage;
|
1581
|
+
}
|
1582
|
+
|
1583
|
+
Coordinate getVirtualCoverage(Node * node)
|
1584
|
+
{
|
1585
|
+
return node->virtualCoverage;
|
1586
|
+
}
|
1587
|
+
|
1588
|
+
Coordinate getTotalCoverage(Node * node)
|
1589
|
+
{
|
1590
|
+
return node->virtualCoverage;
|
1591
|
+
}
|
1592
|
+
|
1593
|
+
#endif
|
1594
|
+
|
1595
|
+
boolean hasSingleArc(Node * node)
|
1596
|
+
{
|
1597
|
+
return node->arcCount == 1;
|
1598
|
+
}
|
1599
|
+
|
1600
|
+
void activateArcLookupTable(Graph * graph)
|
1601
|
+
{
|
1602
|
+
IDnum index;
|
1603
|
+
Node *node;
|
1604
|
+
Arc *arc;
|
1605
|
+
IDnum nodes = graph->nodeCount;
|
1606
|
+
IDnum twinOriginID, destinationID, hash;
|
1607
|
+
Arc **table;
|
1608
|
+
|
1609
|
+
velvetLog("Activating arc lookup table\n");
|
1610
|
+
|
1611
|
+
graph->arcLookupTable = callocOrExit(6 * nodes + 1, Arc *);
|
1612
|
+
|
1613
|
+
table = graph->arcLookupTable;
|
1614
|
+
|
1615
|
+
for (index = -nodes; index <= nodes; index++) {
|
1616
|
+
if (index == 0)
|
1617
|
+
continue;
|
1618
|
+
|
1619
|
+
node = getNodeInGraph(graph, index);
|
1620
|
+
if (node == 0)
|
1621
|
+
continue;
|
1622
|
+
|
1623
|
+
for (arc = getArc(node); arc != NULL;
|
1624
|
+
arc = getNextArc(arc)) {
|
1625
|
+
twinOriginID = arc->twinArc->destination->ID;
|
1626
|
+
destinationID = arc->destination->ID;
|
1627
|
+
hash =
|
1628
|
+
3 * nodes - 2 * twinOriginID + destinationID;
|
1629
|
+
arc->nextInLookupTable = table[hash];
|
1630
|
+
table[hash] = arc;
|
1631
|
+
}
|
1632
|
+
}
|
1633
|
+
|
1634
|
+
velvetLog("Done activating arc lookup table\n");
|
1635
|
+
}
|
1636
|
+
|
1637
|
+
void deactivateArcLookupTable(Graph * graph)
|
1638
|
+
{
|
1639
|
+
free(graph->arcLookupTable);
|
1640
|
+
graph->arcLookupTable = NULL;
|
1641
|
+
}
|
1642
|
+
|
1643
|
+
static void exportNode(FILE * outfile, Node * node, void *withSequence)
|
1644
|
+
{
|
1645
|
+
Coordinate index;
|
1646
|
+
Nucleotide nucleotide;
|
1647
|
+
|
1648
|
+
if (node == NULL)
|
1649
|
+
return;
|
1650
|
+
|
1651
|
+
velvetFprintf(outfile, "NODE\t%ld\t%lld", (long) node->ID, (long long) node->length);
|
1652
|
+
|
1653
|
+
#ifndef SINGLE_COV_CAT
|
1654
|
+
Category cat;
|
1655
|
+
for (cat = 0; cat < CATEGORIES; cat++)
|
1656
|
+
velvetFprintf(outfile, "\t%lld\t%lld", (long long) node->virtualCoverage[cat],
|
1657
|
+
(long long) node->originalVirtualCoverage[cat]);
|
1658
|
+
velvetFprintf(outfile, "\n");
|
1659
|
+
#else
|
1660
|
+
velvetFprintf(outfile, "\t%lld\n", (long long) node->virtualCoverage);
|
1661
|
+
#endif
|
1662
|
+
|
1663
|
+
if (withSequence == NULL)
|
1664
|
+
return;
|
1665
|
+
|
1666
|
+
for (index = 0; index < node->length; index++) {
|
1667
|
+
nucleotide =
|
1668
|
+
getNucleotideInDescriptor(node->descriptor, index);
|
1669
|
+
switch (nucleotide) {
|
1670
|
+
case ADENINE:
|
1671
|
+
velvetFprintf(outfile, "A");
|
1672
|
+
break;
|
1673
|
+
case CYTOSINE:
|
1674
|
+
velvetFprintf(outfile, "C");
|
1675
|
+
break;
|
1676
|
+
case GUANINE:
|
1677
|
+
velvetFprintf(outfile, "G");
|
1678
|
+
break;
|
1679
|
+
case THYMINE:
|
1680
|
+
velvetFprintf(outfile, "T");
|
1681
|
+
break;
|
1682
|
+
}
|
1683
|
+
}
|
1684
|
+
velvetFprintf(outfile, "\n");
|
1685
|
+
|
1686
|
+
for (index = 0; index < node->length; index++) {
|
1687
|
+
nucleotide =
|
1688
|
+
getNucleotideInDescriptor(node->twinNode->descriptor,
|
1689
|
+
index);
|
1690
|
+
switch (nucleotide) {
|
1691
|
+
case ADENINE:
|
1692
|
+
velvetFprintf(outfile, "A");
|
1693
|
+
break;
|
1694
|
+
case CYTOSINE:
|
1695
|
+
velvetFprintf(outfile, "C");
|
1696
|
+
break;
|
1697
|
+
case GUANINE:
|
1698
|
+
velvetFprintf(outfile, "G");
|
1699
|
+
break;
|
1700
|
+
case THYMINE:
|
1701
|
+
velvetFprintf(outfile, "T");
|
1702
|
+
break;
|
1703
|
+
}
|
1704
|
+
}
|
1705
|
+
velvetFprintf(outfile, "\n");
|
1706
|
+
}
|
1707
|
+
|
1708
|
+
static void exportArc(FILE * outfile, Arc * arc)
|
1709
|
+
{
|
1710
|
+
IDnum originID, destinationID;
|
1711
|
+
IDnum absOriginID, absDestinationID;
|
1712
|
+
|
1713
|
+
if (arc == NULL)
|
1714
|
+
return;
|
1715
|
+
|
1716
|
+
absOriginID = originID = -arc->twinArc->destination->ID;
|
1717
|
+
absDestinationID = destinationID = arc->destination->ID;
|
1718
|
+
|
1719
|
+
if (absOriginID < 0)
|
1720
|
+
absOriginID = -absOriginID;
|
1721
|
+
if (absDestinationID < 0)
|
1722
|
+
absDestinationID = -absDestinationID;
|
1723
|
+
|
1724
|
+
if (absDestinationID < absOriginID)
|
1725
|
+
return;
|
1726
|
+
|
1727
|
+
if (originID == destinationID && originID < 0)
|
1728
|
+
return;
|
1729
|
+
|
1730
|
+
velvetFprintf(outfile, "ARC\t%li\t%li\t%li\n", (long) originID, (long) destinationID,
|
1731
|
+
(long) arc->multiplicity);
|
1732
|
+
}
|
1733
|
+
|
1734
|
+
// Merges two lists of annotations in order of increasing position (used in mergeSort mainly)
|
1735
|
+
static Arc *mergeArcLists(Arc * left, Arc * right)
|
1736
|
+
{
|
1737
|
+
Arc *mergedList = NULL;
|
1738
|
+
Arc *tail = NULL;
|
1739
|
+
|
1740
|
+
// Choose first element:
|
1741
|
+
if (left->destination->ID <= right->destination->ID) {
|
1742
|
+
mergedList = left;
|
1743
|
+
tail = left;
|
1744
|
+
left = left->next;
|
1745
|
+
} else {
|
1746
|
+
mergedList = right;
|
1747
|
+
tail = right;
|
1748
|
+
right = right->next;
|
1749
|
+
}
|
1750
|
+
|
1751
|
+
// Iterate while both lists are still non empty
|
1752
|
+
while (left != NULL && right != NULL) {
|
1753
|
+
if (left->destination->ID <= right->destination->ID) {
|
1754
|
+
tail->next = left;
|
1755
|
+
left->previous = tail;
|
1756
|
+
left = left->next;
|
1757
|
+
} else {
|
1758
|
+
tail->next = right;
|
1759
|
+
right->previous = tail;
|
1760
|
+
right = right->next;
|
1761
|
+
}
|
1762
|
+
|
1763
|
+
tail = tail->next;
|
1764
|
+
}
|
1765
|
+
|
1766
|
+
// Concatenate the remaining list at the end of the merged list
|
1767
|
+
if (left != NULL) {
|
1768
|
+
tail->next = left;
|
1769
|
+
left->previous = tail;
|
1770
|
+
}
|
1771
|
+
|
1772
|
+
if (right != NULL) {
|
1773
|
+
tail->next = right;
|
1774
|
+
right->previous = tail;
|
1775
|
+
}
|
1776
|
+
|
1777
|
+
return mergedList;
|
1778
|
+
}
|
1779
|
+
|
1780
|
+
static void arcMergeSort(Arc ** arcPtr, IDnum count)
|
1781
|
+
{
|
1782
|
+
|
1783
|
+
IDnum half = count / 2;
|
1784
|
+
Arc *left = *arcPtr;
|
1785
|
+
Arc *ptr = left;
|
1786
|
+
Arc *right;
|
1787
|
+
IDnum index;
|
1788
|
+
|
1789
|
+
if (count == 0 || count == 1)
|
1790
|
+
return;
|
1791
|
+
|
1792
|
+
if (count == 2) {
|
1793
|
+
if ((*arcPtr)->destination->ID >
|
1794
|
+
(*arcPtr)->next->destination->ID) {
|
1795
|
+
(*arcPtr)->next->next = *arcPtr;
|
1796
|
+
(*arcPtr)->previous = (*arcPtr)->next;
|
1797
|
+
*arcPtr = (*arcPtr)->next;
|
1798
|
+
(*arcPtr)->next->next = NULL;
|
1799
|
+
(*arcPtr)->previous = NULL;
|
1800
|
+
}
|
1801
|
+
return;
|
1802
|
+
}
|
1803
|
+
|
1804
|
+
for (index = 0; index < half - 1; index++) {
|
1805
|
+
ptr = ptr->next;
|
1806
|
+
if (ptr == NULL)
|
1807
|
+
return;
|
1808
|
+
}
|
1809
|
+
|
1810
|
+
right = ptr->next;
|
1811
|
+
ptr->next = NULL;
|
1812
|
+
right->previous = NULL;
|
1813
|
+
|
1814
|
+
arcMergeSort(&left, half);
|
1815
|
+
arcMergeSort(&right, count - half);
|
1816
|
+
*arcPtr = mergeArcLists(left, right);
|
1817
|
+
}
|
1818
|
+
|
1819
|
+
static void sortNodeArcs(Node * node)
|
1820
|
+
{
|
1821
|
+
Arc *arc;
|
1822
|
+
IDnum count = 0;
|
1823
|
+
|
1824
|
+
for (arc = getArc(node); arc != NULL; arc = getNextArc(arc))
|
1825
|
+
count++;
|
1826
|
+
|
1827
|
+
if (count == 0)
|
1828
|
+
return;
|
1829
|
+
|
1830
|
+
arc = getArc(node);
|
1831
|
+
arcMergeSort(&arc, count);
|
1832
|
+
|
1833
|
+
node->arc = arc;
|
1834
|
+
}
|
1835
|
+
|
1836
|
+
// Merges two lists of annotations in order of increasing position (used in mergeSort mainly)
|
1837
|
+
static GapMarker *mergeGapMarkerLists(GapMarker * left, GapMarker * right)
|
1838
|
+
{
|
1839
|
+
GapMarker *mergedList = NULL;
|
1840
|
+
GapMarker *tail = NULL;
|
1841
|
+
|
1842
|
+
// Choose first element:
|
1843
|
+
if (left->position <= right->position) {
|
1844
|
+
mergedList = left;
|
1845
|
+
tail = left;
|
1846
|
+
left = left->next;
|
1847
|
+
} else {
|
1848
|
+
mergedList = right;
|
1849
|
+
tail = right;
|
1850
|
+
right = right->next;
|
1851
|
+
}
|
1852
|
+
|
1853
|
+
// Iterate while both lists are still non empty
|
1854
|
+
while (left != NULL && right != NULL) {
|
1855
|
+
if (left->position <= right->position) {
|
1856
|
+
tail->next = left;
|
1857
|
+
left = left->next;
|
1858
|
+
} else {
|
1859
|
+
tail->next = right;
|
1860
|
+
right = right->next;
|
1861
|
+
}
|
1862
|
+
|
1863
|
+
tail = tail->next;
|
1864
|
+
}
|
1865
|
+
|
1866
|
+
// Concatenate the remaining list at the end of the merged list
|
1867
|
+
if (left != NULL)
|
1868
|
+
tail->next = left;
|
1869
|
+
|
1870
|
+
if (right != NULL)
|
1871
|
+
tail->next = right;
|
1872
|
+
|
1873
|
+
return mergedList;
|
1874
|
+
}
|
1875
|
+
|
1876
|
+
static void gapMergeSort(GapMarker ** gapPtr, IDnum count)
|
1877
|
+
{
|
1878
|
+
|
1879
|
+
IDnum half = count / 2;
|
1880
|
+
GapMarker *left = *gapPtr;
|
1881
|
+
GapMarker *ptr = left;
|
1882
|
+
GapMarker *right;
|
1883
|
+
IDnum index;
|
1884
|
+
|
1885
|
+
if (count == 0 || count == 1)
|
1886
|
+
return;
|
1887
|
+
|
1888
|
+
if (count == 2) {
|
1889
|
+
if ((*gapPtr)->position > (*gapPtr)->next->position) {
|
1890
|
+
(*gapPtr)->next->next = *gapPtr;
|
1891
|
+
*gapPtr = (*gapPtr)->next;
|
1892
|
+
(*gapPtr)->next->next = NULL;
|
1893
|
+
}
|
1894
|
+
return;
|
1895
|
+
}
|
1896
|
+
|
1897
|
+
for (index = 0; index < half - 1; index++) {
|
1898
|
+
ptr = ptr->next;
|
1899
|
+
if (ptr == NULL)
|
1900
|
+
return;
|
1901
|
+
}
|
1902
|
+
|
1903
|
+
right = ptr->next;
|
1904
|
+
ptr->next = NULL;
|
1905
|
+
|
1906
|
+
gapMergeSort(&left, half);
|
1907
|
+
gapMergeSort(&right, count - half);
|
1908
|
+
*gapPtr = mergeGapMarkerLists(left, right);
|
1909
|
+
}
|
1910
|
+
|
1911
|
+
static void sortNodeGapMarkers(Node * node, Graph * graph)
|
1912
|
+
{
|
1913
|
+
GapMarker *gap;
|
1914
|
+
IDnum count = 0;
|
1915
|
+
IDnum ID = getNodeID(node);
|
1916
|
+
|
1917
|
+
if (ID < 0)
|
1918
|
+
ID = -ID;
|
1919
|
+
|
1920
|
+
for (gap = graph->gapMarkers[ID]; gap != NULL; gap = gap->next)
|
1921
|
+
count++;
|
1922
|
+
|
1923
|
+
if (count == 0)
|
1924
|
+
return;
|
1925
|
+
|
1926
|
+
gap = graph->gapMarkers[ID];
|
1927
|
+
gapMergeSort(&gap, count);
|
1928
|
+
|
1929
|
+
graph->gapMarkers[ID] = gap;
|
1930
|
+
}
|
1931
|
+
|
1932
|
+
void sortGapMarkers(Graph * graph)
|
1933
|
+
{
|
1934
|
+
IDnum index;
|
1935
|
+
Node *node;
|
1936
|
+
|
1937
|
+
if (graph->gapMarkers == NULL)
|
1938
|
+
return;
|
1939
|
+
|
1940
|
+
for (index = 1; index <= nodeCount(graph); index++) {
|
1941
|
+
node = getNodeInGraph(graph, index);
|
1942
|
+
if (node)
|
1943
|
+
sortNodeGapMarkers(node, graph);
|
1944
|
+
}
|
1945
|
+
}
|
1946
|
+
|
1947
|
+
void exportGraph(char *filename, Graph * graph, TightString * sequences)
|
1948
|
+
{
|
1949
|
+
IDnum index;
|
1950
|
+
FILE *outfile;
|
1951
|
+
Node *node;
|
1952
|
+
Arc *arc;
|
1953
|
+
PassageMarkerI marker;
|
1954
|
+
ShortReadMarker *reads;
|
1955
|
+
IDnum readCount, readIndex;
|
1956
|
+
|
1957
|
+
if (graph == NULL) {
|
1958
|
+
return;
|
1959
|
+
}
|
1960
|
+
|
1961
|
+
outfile = fopen(filename, "w");
|
1962
|
+
if (outfile == NULL) {
|
1963
|
+
velvetLog("Couldn't open file, sorry\n");
|
1964
|
+
return;
|
1965
|
+
} else
|
1966
|
+
velvetLog("Writing into graph file %s...\n", filename);
|
1967
|
+
|
1968
|
+
// General data
|
1969
|
+
velvetFprintf(outfile, "%li\t%li\t%i\t%i\n", (long) graph->nodeCount,
|
1970
|
+
(long) graph->sequenceCount, graph->wordLength, (int) graph->double_stranded);
|
1971
|
+
|
1972
|
+
// Node info
|
1973
|
+
for (index = 1; index <= graph->nodeCount; index++) {
|
1974
|
+
node = getNodeInGraph(graph, index);
|
1975
|
+
exportNode(outfile, node, (void *) sequences);
|
1976
|
+
}
|
1977
|
+
|
1978
|
+
// Arc info
|
1979
|
+
for (index = 1; index <= graph->nodeCount; index++) {
|
1980
|
+
node = getNodeInGraph(graph, index);
|
1981
|
+
if (node == NULL)
|
1982
|
+
continue;
|
1983
|
+
|
1984
|
+
sortNodeArcs(node);
|
1985
|
+
sortNodeArcs(getTwinNode(node));
|
1986
|
+
|
1987
|
+
for (arc = node->arc; arc != NULL; arc = arc->next)
|
1988
|
+
exportArc(outfile, arc);
|
1989
|
+
for (arc = node->twinNode->arc; arc != NULL;
|
1990
|
+
arc = arc->next)
|
1991
|
+
exportArc(outfile, arc);
|
1992
|
+
}
|
1993
|
+
|
1994
|
+
// Sequence info
|
1995
|
+
for (index = 1; index <= graph->nodeCount; index++) {
|
1996
|
+
node = getNodeInGraph(graph, index);
|
1997
|
+
if (node == NULL)
|
1998
|
+
continue;
|
1999
|
+
for (marker = node->marker; marker != NULL_IDX;
|
2000
|
+
marker = getNextInNode(marker))
|
2001
|
+
exportMarker(outfile, marker, sequences,
|
2002
|
+
graph->wordLength);
|
2003
|
+
}
|
2004
|
+
|
2005
|
+
// Node reads
|
2006
|
+
if (readStartsAreActivated(graph)) {
|
2007
|
+
for (index = 0; index <= graph->nodeCount * 2; index++) {
|
2008
|
+
readCount = graph->nodeReadCounts[index];
|
2009
|
+
if (readCount == 0)
|
2010
|
+
continue;
|
2011
|
+
|
2012
|
+
velvetFprintf(outfile, "NR\t%li\t%li\n",
|
2013
|
+
(long) (index - graph->nodeCount), (long) readCount);
|
2014
|
+
|
2015
|
+
reads = graph->nodeReads[index];
|
2016
|
+
for (readIndex = 0; readIndex < readCount;
|
2017
|
+
readIndex++)
|
2018
|
+
velvetFprintf(outfile, "%ld\t%lld\t%d\n",
|
2019
|
+
(long) reads[readIndex].readID,
|
2020
|
+
(long long) reads[readIndex].position,
|
2021
|
+
(int) reads[readIndex].offset);
|
2022
|
+
}
|
2023
|
+
}
|
2024
|
+
|
2025
|
+
fclose(outfile);
|
2026
|
+
}
|
2027
|
+
|
2028
|
+
Graph *importGraph(char *filename)
|
2029
|
+
{
|
2030
|
+
FILE *file = fopen(filename, "r");
|
2031
|
+
const int maxline = MAXLINE;
|
2032
|
+
char line[MAXLINE];
|
2033
|
+
Graph *graph;
|
2034
|
+
Coordinate coverage;
|
2035
|
+
IDnum nodeCounter, sequenceCount;
|
2036
|
+
Node *node, *twin;
|
2037
|
+
Arc *arc;
|
2038
|
+
IDnum originID, destinationID, multiplicity;
|
2039
|
+
PassageMarkerI newMarker, marker;
|
2040
|
+
IDnum nodeID, seqID;
|
2041
|
+
Coordinate index;
|
2042
|
+
Coordinate start, finish;
|
2043
|
+
Coordinate startOffset, finishOffset;
|
2044
|
+
boolean finished = false;
|
2045
|
+
size_t arrayLength;
|
2046
|
+
IDnum readCount;
|
2047
|
+
ShortReadMarker *array;
|
2048
|
+
int wordLength, sCount;
|
2049
|
+
ShortLength length;
|
2050
|
+
long long_var, long_var2, long_var3;
|
2051
|
+
long long longlong_var, longlong_var2, longlong_var3, longlong_var4;
|
2052
|
+
short short_var;
|
2053
|
+
char c;
|
2054
|
+
|
2055
|
+
if (file == NULL)
|
2056
|
+
exitErrorf(EXIT_FAILURE, true, "Could not open %s", filename);
|
2057
|
+
|
2058
|
+
velvetLog("Reading graph file %s\n", filename);
|
2059
|
+
|
2060
|
+
// First line
|
2061
|
+
if (!fgets(line, maxline, file))
|
2062
|
+
exitErrorf(EXIT_FAILURE, true, "Graph file incomplete");
|
2063
|
+
sscanf(line, "%ld\t%ld\t%i\t%hi\n", &long_var, &long_var2,
|
2064
|
+
&wordLength, &short_var);
|
2065
|
+
nodeCounter = (IDnum) long_var;
|
2066
|
+
sequenceCount = (IDnum) long_var2;
|
2067
|
+
graph = emptyGraph(sequenceCount, wordLength);
|
2068
|
+
graph->double_stranded = (boolean) short_var;
|
2069
|
+
resetWordFilter(wordLength);
|
2070
|
+
allocateNodeSpace(graph, nodeCounter);
|
2071
|
+
|
2072
|
+
velvetLog("Graph has %ld nodes and %ld sequences\n", (long) nodeCounter,
|
2073
|
+
(long) sequenceCount);
|
2074
|
+
|
2075
|
+
if (nodeCounter == 0)
|
2076
|
+
return graph;
|
2077
|
+
|
2078
|
+
// Read nodes
|
2079
|
+
if (!fgets(line, maxline, file))
|
2080
|
+
exitErrorf(EXIT_FAILURE, true, "Graph file incomplete");
|
2081
|
+
while (!finished && strncmp(line, "NODE", 4) == 0) {
|
2082
|
+
strtok(line, "\t\n");
|
2083
|
+
sscanf(strtok(NULL, "\t\n"), "%ld", &long_var);
|
2084
|
+
nodeID = (IDnum) long_var;
|
2085
|
+
node = addEmptyNodeToGraph(graph, nodeID);
|
2086
|
+
sscanf(strtok(NULL, "\t\n"), "%lld", &longlong_var);
|
2087
|
+
node->length = (Coordinate) longlong_var;
|
2088
|
+
|
2089
|
+
#ifndef SINGLE_COV_CAT
|
2090
|
+
Category cat;
|
2091
|
+
Coordinate originalCoverage;
|
2092
|
+
for (cat = 0; cat < CATEGORIES; cat++) {
|
2093
|
+
sscanf(strtok(NULL, "\t\n"), "%lld", &longlong_var);
|
2094
|
+
coverage = (Coordinate) longlong_var;
|
2095
|
+
setVirtualCoverage(node, cat, coverage);
|
2096
|
+
sscanf(strtok(NULL, "\t\n"), "%lld",
|
2097
|
+
&longlong_var);
|
2098
|
+
originalCoverage = (Coordinate) longlong_var;
|
2099
|
+
setOriginalVirtualCoverage(node, cat,
|
2100
|
+
originalCoverage);
|
2101
|
+
}
|
2102
|
+
#else
|
2103
|
+
sscanf(strtok(NULL, "\t\n"), "%lld", &longlong_var);
|
2104
|
+
coverage = (Coordinate) longlong_var;
|
2105
|
+
setVirtualCoverage(node, coverage);
|
2106
|
+
#endif
|
2107
|
+
|
2108
|
+
arrayLength = node->length / 4;
|
2109
|
+
if (node->length % 4 > 0)
|
2110
|
+
arrayLength++;
|
2111
|
+
node->descriptor =
|
2112
|
+
callocOrExit(arrayLength, Descriptor);
|
2113
|
+
|
2114
|
+
index = 0;
|
2115
|
+
while ((c = fgetc(file)) != '\n' && c != EOF) {
|
2116
|
+
if (c == 'A')
|
2117
|
+
writeNucleotideInDescriptor(ADENINE,
|
2118
|
+
node->
|
2119
|
+
descriptor,
|
2120
|
+
index++);
|
2121
|
+
else if (c == 'C')
|
2122
|
+
writeNucleotideInDescriptor(CYTOSINE,
|
2123
|
+
node->
|
2124
|
+
descriptor,
|
2125
|
+
index++);
|
2126
|
+
else if (c == 'G')
|
2127
|
+
writeNucleotideInDescriptor(GUANINE,
|
2128
|
+
node->
|
2129
|
+
descriptor,
|
2130
|
+
index++);
|
2131
|
+
else if (c == 'T')
|
2132
|
+
writeNucleotideInDescriptor(THYMINE,
|
2133
|
+
node->
|
2134
|
+
descriptor,
|
2135
|
+
index++);
|
2136
|
+
}
|
2137
|
+
|
2138
|
+
twin = node->twinNode;
|
2139
|
+
twin->length = node->length;
|
2140
|
+
twin->descriptor =
|
2141
|
+
callocOrExit(arrayLength, Descriptor);
|
2142
|
+
index = 0;
|
2143
|
+
while ((c = fgetc(file)) != '\n' && c != EOF) {
|
2144
|
+
if (c == 'A')
|
2145
|
+
writeNucleotideInDescriptor(ADENINE,
|
2146
|
+
twin->
|
2147
|
+
descriptor,
|
2148
|
+
index++);
|
2149
|
+
else if (c == 'C')
|
2150
|
+
writeNucleotideInDescriptor(CYTOSINE,
|
2151
|
+
twin->
|
2152
|
+
descriptor,
|
2153
|
+
index++);
|
2154
|
+
else if (c == 'G')
|
2155
|
+
writeNucleotideInDescriptor(GUANINE,
|
2156
|
+
twin->
|
2157
|
+
descriptor,
|
2158
|
+
index++);
|
2159
|
+
else if (c == 'T')
|
2160
|
+
writeNucleotideInDescriptor(THYMINE,
|
2161
|
+
twin->
|
2162
|
+
descriptor,
|
2163
|
+
index++);
|
2164
|
+
}
|
2165
|
+
|
2166
|
+
if (fgets(line, maxline, file) == NULL)
|
2167
|
+
finished = true;
|
2168
|
+
}
|
2169
|
+
|
2170
|
+
// Read arcs
|
2171
|
+
while (!finished && line[0] == 'A') {
|
2172
|
+
sscanf(line, "ARC\t%ld\t%ld\t%ld\n", &long_var,
|
2173
|
+
&long_var2, &long_var3);
|
2174
|
+
originID = (IDnum) long_var;
|
2175
|
+
destinationID = (IDnum) long_var2;
|
2176
|
+
multiplicity = (IDnum) long_var3;
|
2177
|
+
arc =
|
2178
|
+
createArc(getNodeInGraph(graph, originID),
|
2179
|
+
getNodeInGraph(graph, destinationID), graph);
|
2180
|
+
setMultiplicity(arc, multiplicity);
|
2181
|
+
if (fgets(line, maxline, file) == NULL)
|
2182
|
+
finished = true;
|
2183
|
+
}
|
2184
|
+
|
2185
|
+
// Read sequences
|
2186
|
+
while (!finished && line[0] != 'N') {
|
2187
|
+
sscanf(line, "SEQ\t%ld\n", &long_var);
|
2188
|
+
seqID = (IDnum) long_var;
|
2189
|
+
marker = NULL_IDX;
|
2190
|
+
if (!fgets(line, maxline, file))
|
2191
|
+
exitErrorf(EXIT_FAILURE, true, "Graph file incomplete");
|
2192
|
+
|
2193
|
+
while (!finished && line[0] != 'N' && line[0] != 'S') {
|
2194
|
+
sCount =
|
2195
|
+
sscanf(line, "%ld\t%lld\t%lld\t%lld\t%lld\n",
|
2196
|
+
&long_var, &longlong_var, &longlong_var2, &longlong_var3,
|
2197
|
+
&longlong_var4);
|
2198
|
+
nodeID = (IDnum) long_var;
|
2199
|
+
startOffset = (Coordinate) longlong_var;
|
2200
|
+
start = (Coordinate) longlong_var2;
|
2201
|
+
finish = (Coordinate) longlong_var3;
|
2202
|
+
finishOffset = (Coordinate) longlong_var4;
|
2203
|
+
if (sCount != 5) {
|
2204
|
+
velvetLog
|
2205
|
+
("ERROR: reading in graph - only %d items read for line '%s'",
|
2206
|
+
sCount, line);
|
2207
|
+
#ifdef DEBUG
|
2208
|
+
abort();
|
2209
|
+
#endif
|
2210
|
+
exit(1);
|
2211
|
+
}
|
2212
|
+
newMarker =
|
2213
|
+
newPassageMarker(seqID, start, finish,
|
2214
|
+
startOffset, finishOffset);
|
2215
|
+
transposePassageMarker(newMarker,
|
2216
|
+
getNodeInGraph(graph,
|
2217
|
+
nodeID));
|
2218
|
+
connectPassageMarkers(marker, newMarker, graph);
|
2219
|
+
marker = newMarker;
|
2220
|
+
if (fgets(line, maxline, file) == NULL)
|
2221
|
+
finished = true;
|
2222
|
+
}
|
2223
|
+
}
|
2224
|
+
|
2225
|
+
// Node reads
|
2226
|
+
while (!finished) {
|
2227
|
+
sscanf(line, "NR\t%ld\t%ld\n", &long_var, &long_var2);
|
2228
|
+
nodeID = (IDnum) long_var;
|
2229
|
+
readCount = (IDnum) long_var2;
|
2230
|
+
if (!readStartsAreActivated(graph))
|
2231
|
+
activateReadStarts(graph);
|
2232
|
+
|
2233
|
+
graph->nodeReadCounts[nodeID + graph->nodeCount] =
|
2234
|
+
readCount;
|
2235
|
+
array = mallocOrExit(readCount, ShortReadMarker);
|
2236
|
+
graph->nodeReads[nodeID + graph->nodeCount] = array;
|
2237
|
+
|
2238
|
+
readCount = 0;
|
2239
|
+
if (!fgets(line, maxline, file))
|
2240
|
+
exitErrorf(EXIT_FAILURE, true, "Graph file incomplete");
|
2241
|
+
while (!finished && line[0] != 'N') {
|
2242
|
+
sscanf(line, "%ld\t%lld\t%hd\n", &long_var,
|
2243
|
+
&longlong_var, &short_var);
|
2244
|
+
seqID = (IDnum) long_var;
|
2245
|
+
startOffset = (Coordinate) longlong_var;
|
2246
|
+
length = (ShortLength) short_var;
|
2247
|
+
array[readCount].readID = seqID;
|
2248
|
+
array[readCount].position = startOffset;
|
2249
|
+
array[readCount].offset = length;
|
2250
|
+
readCount++;
|
2251
|
+
if (fgets(line, maxline, file) == NULL)
|
2252
|
+
finished = true;
|
2253
|
+
}
|
2254
|
+
}
|
2255
|
+
|
2256
|
+
//velvetLog("New graph has %d nodes\n", graph->nodeCount);
|
2257
|
+
|
2258
|
+
fclose(file);
|
2259
|
+
//velvetLog("Done, exiting\n");
|
2260
|
+
return graph;
|
2261
|
+
}
|
2262
|
+
|
2263
|
+
Graph *readPreGraphFile(char *preGraphFilename, boolean * double_strand)
|
2264
|
+
{
|
2265
|
+
FILE *file = fopen(preGraphFilename, "r");
|
2266
|
+
const int maxline = MAXLINE;
|
2267
|
+
char line[MAXLINE];
|
2268
|
+
|
2269
|
+
Graph *graph;
|
2270
|
+
IDnum nodeCounter, sequenceCount;
|
2271
|
+
|
2272
|
+
Node *node, *twin;
|
2273
|
+
IDnum nodeID = 0;
|
2274
|
+
Coordinate index, nodeLength;
|
2275
|
+
char c;
|
2276
|
+
int wordLength, wordShift;
|
2277
|
+
size_t arrayLength;
|
2278
|
+
short short_var;
|
2279
|
+
long long_var, long_var2;
|
2280
|
+
long long longlong_var;
|
2281
|
+
|
2282
|
+
if (file == NULL)
|
2283
|
+
exitErrorf(EXIT_FAILURE, true, "Could not open %s", preGraphFilename);
|
2284
|
+
|
2285
|
+
velvetLog("Reading pre-graph file %s\n", preGraphFilename);
|
2286
|
+
|
2287
|
+
// First line
|
2288
|
+
if (!fgets(line, maxline, file))
|
2289
|
+
exitErrorf(EXIT_FAILURE, true, "PreGraph file incomplete");
|
2290
|
+
sscanf(line, "%ld\t%ld\t%i\t%hi\n", &long_var, &long_var2,
|
2291
|
+
&wordLength, &short_var);
|
2292
|
+
nodeCounter = (IDnum) long_var;
|
2293
|
+
sequenceCount = (IDnum) long_var2;
|
2294
|
+
*double_strand = (boolean) short_var;
|
2295
|
+
wordShift = wordLength - 1;
|
2296
|
+
graph = emptyGraph(sequenceCount, wordLength);
|
2297
|
+
graph->double_stranded = *double_strand;
|
2298
|
+
resetWordFilter(wordLength);
|
2299
|
+
allocateNodeSpace(graph, nodeCounter);
|
2300
|
+
velvetLog("Graph has %ld nodes and %ld sequences\n", (long) nodeCounter,
|
2301
|
+
(long) sequenceCount);
|
2302
|
+
|
2303
|
+
// Read nodes
|
2304
|
+
if (nodeCounter == 0)
|
2305
|
+
return graph;
|
2306
|
+
|
2307
|
+
if (!fgets(line, maxline, file))
|
2308
|
+
exitErrorf(EXIT_FAILURE, true, "PreGraph file incomplete");
|
2309
|
+
while (line[0] == 'N') {
|
2310
|
+
nodeID++;
|
2311
|
+
node = addEmptyNodeToGraph(graph, nodeID);
|
2312
|
+
|
2313
|
+
sscanf(line, "%*s\t%*i\t%lli\n", &longlong_var);
|
2314
|
+
node->length = (Coordinate) longlong_var;
|
2315
|
+
nodeLength = node->length;
|
2316
|
+
arrayLength = node->length / 4;
|
2317
|
+
if (node->length % 4 > 0)
|
2318
|
+
arrayLength++;
|
2319
|
+
node->descriptor =
|
2320
|
+
callocOrExit(arrayLength, Descriptor);
|
2321
|
+
|
2322
|
+
twin = node->twinNode;
|
2323
|
+
twin->length = nodeLength;
|
2324
|
+
twin->descriptor =
|
2325
|
+
callocOrExit(arrayLength, Descriptor);
|
2326
|
+
|
2327
|
+
|
2328
|
+
index = 0;
|
2329
|
+
while ((c = getc(file)) != '\n') {
|
2330
|
+
if (c == 'A') {
|
2331
|
+
if (index - wordShift >= 0)
|
2332
|
+
writeNucleotideInDescriptor(ADENINE,
|
2333
|
+
node->
|
2334
|
+
descriptor,
|
2335
|
+
index - wordShift);
|
2336
|
+
if (nodeLength - index - 1 >= 0) {
|
2337
|
+
#ifndef COLOR
|
2338
|
+
writeNucleotideInDescriptor(THYMINE,
|
2339
|
+
twin->
|
2340
|
+
descriptor,
|
2341
|
+
nodeLength - index - 1);
|
2342
|
+
#else
|
2343
|
+
writeNucleotideInDescriptor(ADENINE,
|
2344
|
+
twin->
|
2345
|
+
descriptor,
|
2346
|
+
nodeLength - index - 1);
|
2347
|
+
#endif
|
2348
|
+
}
|
2349
|
+
} else if (c == 'C') {
|
2350
|
+
if (index - wordShift >= 0)
|
2351
|
+
writeNucleotideInDescriptor(CYTOSINE,
|
2352
|
+
node->
|
2353
|
+
descriptor,
|
2354
|
+
index - wordShift);
|
2355
|
+
if (nodeLength - index - 1 >= 0) {
|
2356
|
+
#ifndef COLOR
|
2357
|
+
writeNucleotideInDescriptor(GUANINE,
|
2358
|
+
twin->
|
2359
|
+
descriptor,
|
2360
|
+
nodeLength - index - 1);
|
2361
|
+
#else
|
2362
|
+
writeNucleotideInDescriptor(CYTOSINE,
|
2363
|
+
twin->
|
2364
|
+
descriptor,
|
2365
|
+
nodeLength - index - 1);
|
2366
|
+
#endif
|
2367
|
+
}
|
2368
|
+
} else if (c == 'G') {
|
2369
|
+
if (index - wordShift >= 0)
|
2370
|
+
writeNucleotideInDescriptor(GUANINE,
|
2371
|
+
node->
|
2372
|
+
descriptor,
|
2373
|
+
index - wordShift);
|
2374
|
+
if (nodeLength - index - 1 >= 0) {
|
2375
|
+
#ifndef COLOR
|
2376
|
+
writeNucleotideInDescriptor(CYTOSINE,
|
2377
|
+
twin->
|
2378
|
+
descriptor,
|
2379
|
+
nodeLength - index - 1);
|
2380
|
+
#else
|
2381
|
+
writeNucleotideInDescriptor(GUANINE,
|
2382
|
+
twin->
|
2383
|
+
descriptor,
|
2384
|
+
nodeLength - index - 1);
|
2385
|
+
#endif
|
2386
|
+
}
|
2387
|
+
} else if (c == 'T') {
|
2388
|
+
if (index - wordShift >= 0)
|
2389
|
+
writeNucleotideInDescriptor(THYMINE,
|
2390
|
+
node->
|
2391
|
+
descriptor,
|
2392
|
+
index - wordShift);
|
2393
|
+
if (nodeLength - index - 1 >= 0) {
|
2394
|
+
#ifndef COLOR
|
2395
|
+
writeNucleotideInDescriptor(ADENINE,
|
2396
|
+
twin->
|
2397
|
+
descriptor,
|
2398
|
+
nodeLength - index - 1);
|
2399
|
+
#else
|
2400
|
+
writeNucleotideInDescriptor(THYMINE,
|
2401
|
+
twin->
|
2402
|
+
descriptor,
|
2403
|
+
nodeLength - index - 1);
|
2404
|
+
#endif
|
2405
|
+
}
|
2406
|
+
}
|
2407
|
+
|
2408
|
+
index++;
|
2409
|
+
}
|
2410
|
+
|
2411
|
+
if (fgets(line, maxline, file) == NULL) {
|
2412
|
+
fclose(file);
|
2413
|
+
return graph;
|
2414
|
+
}
|
2415
|
+
}
|
2416
|
+
|
2417
|
+
fclose(file);
|
2418
|
+
return graph;
|
2419
|
+
}
|
2420
|
+
|
2421
|
+
Graph *readConnectedGraphFile(char *connectedGraphFilename, boolean * double_strand)
|
2422
|
+
{
|
2423
|
+
FILE *file = fopen(connectedGraphFilename, "r");
|
2424
|
+
const int maxline = MAXLINE;
|
2425
|
+
char line[MAXLINE];
|
2426
|
+
Coordinate coverage;
|
2427
|
+
Arc *arc;
|
2428
|
+
IDnum originID, destinationID, multiplicity;
|
2429
|
+
boolean finished = false;
|
2430
|
+
long long_var3;
|
2431
|
+
|
2432
|
+
Graph *graph;
|
2433
|
+
IDnum nodeCounter, sequenceCount;
|
2434
|
+
|
2435
|
+
Node *node, *twin;
|
2436
|
+
IDnum nodeID = 0;
|
2437
|
+
Coordinate index, nodeLength;
|
2438
|
+
char c;
|
2439
|
+
int wordLength, wordShift;
|
2440
|
+
size_t arrayLength;
|
2441
|
+
short short_var;
|
2442
|
+
long long_var, long_var2;
|
2443
|
+
long long longlong_var;
|
2444
|
+
|
2445
|
+
if (file == NULL)
|
2446
|
+
exitErrorf(EXIT_FAILURE, true, "Could not open %s", connectedGraphFilename);
|
2447
|
+
|
2448
|
+
velvetLog("Reading connected graph file %s\n", connectedGraphFilename);
|
2449
|
+
|
2450
|
+
// First line
|
2451
|
+
if (!fgets(line, maxline, file))
|
2452
|
+
exitErrorf(EXIT_FAILURE, true, "PreGraph file incomplete");
|
2453
|
+
sscanf(line, "%ld\t%ld\t%i\t%hi\n", &long_var, &long_var2,
|
2454
|
+
&wordLength, &short_var);
|
2455
|
+
nodeCounter = (IDnum) long_var;
|
2456
|
+
sequenceCount = (IDnum) long_var2;
|
2457
|
+
*double_strand = (boolean) short_var;
|
2458
|
+
wordShift = wordLength - 1;
|
2459
|
+
graph = emptyGraph(sequenceCount, wordLength);
|
2460
|
+
graph->double_stranded = *double_strand;
|
2461
|
+
resetWordFilter(wordLength);
|
2462
|
+
allocateNodeSpace(graph, nodeCounter);
|
2463
|
+
velvetLog("Graph has %ld nodes and %ld sequences\n", (long) nodeCounter,
|
2464
|
+
(long) sequenceCount);
|
2465
|
+
|
2466
|
+
// Read nodes
|
2467
|
+
if (nodeCounter == 0)
|
2468
|
+
return graph;
|
2469
|
+
|
2470
|
+
if (!fgets(line, maxline, file))
|
2471
|
+
exitErrorf(EXIT_FAILURE, true, "PreGraph file incomplete");
|
2472
|
+
while (!finished && strncmp(line, "NODE", 4) == 0) {
|
2473
|
+
strtok(line, "\t\n");
|
2474
|
+
sscanf(strtok(NULL, "\t\n"), "%ld", &long_var);
|
2475
|
+
nodeID = (IDnum) long_var;
|
2476
|
+
node = addEmptyNodeToGraph(graph, nodeID);
|
2477
|
+
sscanf(strtok(NULL, "\t\n"), "%lld", &longlong_var);
|
2478
|
+
node->length = (Coordinate) longlong_var;
|
2479
|
+
nodeLength = node->length;
|
2480
|
+
|
2481
|
+
#ifndef SINGLE_COV_CAT
|
2482
|
+
Category cat;
|
2483
|
+
Coordinate originalCoverage;
|
2484
|
+
for (cat = 0; cat < CATEGORIES; cat++) {
|
2485
|
+
sscanf(strtok(NULL, "\t\n"), "%lld", &longlong_var);
|
2486
|
+
coverage = (Coordinate) longlong_var;
|
2487
|
+
setVirtualCoverage(node, cat, coverage);
|
2488
|
+
sscanf(strtok(NULL, "\t\n"), "%lld",
|
2489
|
+
&longlong_var);
|
2490
|
+
originalCoverage = (Coordinate) longlong_var;
|
2491
|
+
setOriginalVirtualCoverage(node, cat,
|
2492
|
+
originalCoverage);
|
2493
|
+
}
|
2494
|
+
#else
|
2495
|
+
sscanf(strtok(NULL, "\t\n"), "%lld", &longlong_var);
|
2496
|
+
coverage = (Coordinate) longlong_var;
|
2497
|
+
setVirtualCoverage(node, coverage);
|
2498
|
+
#endif
|
2499
|
+
|
2500
|
+
arrayLength = node->length / 4;
|
2501
|
+
if (node->length % 4 > 0)
|
2502
|
+
arrayLength++;
|
2503
|
+
node->descriptor =
|
2504
|
+
callocOrExit(arrayLength, Descriptor);
|
2505
|
+
|
2506
|
+
twin = node->twinNode;
|
2507
|
+
twin->length = node->length;
|
2508
|
+
twin->descriptor =
|
2509
|
+
callocOrExit(arrayLength, Descriptor);
|
2510
|
+
|
2511
|
+
index = 0;
|
2512
|
+
while ((c = getc(file)) != '\n') {
|
2513
|
+
if (c == 'A') {
|
2514
|
+
if (index - wordShift >= 0)
|
2515
|
+
writeNucleotideInDescriptor(ADENINE,
|
2516
|
+
node->
|
2517
|
+
descriptor,
|
2518
|
+
index - wordShift);
|
2519
|
+
if (nodeLength - index - 1 >= 0) {
|
2520
|
+
#ifndef COLOR
|
2521
|
+
writeNucleotideInDescriptor(THYMINE,
|
2522
|
+
twin->
|
2523
|
+
descriptor,
|
2524
|
+
nodeLength - index - 1);
|
2525
|
+
#else
|
2526
|
+
writeNucleotideInDescriptor(ADENINE,
|
2527
|
+
twin->
|
2528
|
+
descriptor,
|
2529
|
+
nodeLength - index - 1);
|
2530
|
+
#endif
|
2531
|
+
}
|
2532
|
+
} else if (c == 'C') {
|
2533
|
+
if (index - wordShift >= 0)
|
2534
|
+
writeNucleotideInDescriptor(CYTOSINE,
|
2535
|
+
node->
|
2536
|
+
descriptor,
|
2537
|
+
index - wordShift);
|
2538
|
+
if (nodeLength - index - 1 >= 0) {
|
2539
|
+
#ifndef COLOR
|
2540
|
+
writeNucleotideInDescriptor(GUANINE,
|
2541
|
+
twin->
|
2542
|
+
descriptor,
|
2543
|
+
nodeLength - index - 1);
|
2544
|
+
#else
|
2545
|
+
writeNucleotideInDescriptor(CYTOSINE,
|
2546
|
+
twin->
|
2547
|
+
descriptor,
|
2548
|
+
nodeLength - index - 1);
|
2549
|
+
#endif
|
2550
|
+
}
|
2551
|
+
} else if (c == 'G') {
|
2552
|
+
if (index - wordShift >= 0)
|
2553
|
+
writeNucleotideInDescriptor(GUANINE,
|
2554
|
+
node->
|
2555
|
+
descriptor,
|
2556
|
+
index - wordShift);
|
2557
|
+
if (nodeLength - index - 1 >= 0) {
|
2558
|
+
#ifndef COLOR
|
2559
|
+
writeNucleotideInDescriptor(CYTOSINE,
|
2560
|
+
twin->
|
2561
|
+
descriptor,
|
2562
|
+
nodeLength - index - 1);
|
2563
|
+
#else
|
2564
|
+
writeNucleotideInDescriptor(GUANINE,
|
2565
|
+
twin->
|
2566
|
+
descriptor,
|
2567
|
+
nodeLength - index - 1);
|
2568
|
+
#endif
|
2569
|
+
}
|
2570
|
+
} else if (c == 'T') {
|
2571
|
+
if (index - wordShift >= 0)
|
2572
|
+
writeNucleotideInDescriptor(THYMINE,
|
2573
|
+
node->
|
2574
|
+
descriptor,
|
2575
|
+
index - wordShift);
|
2576
|
+
if (nodeLength - index - 1 >= 0) {
|
2577
|
+
#ifndef COLOR
|
2578
|
+
writeNucleotideInDescriptor(ADENINE,
|
2579
|
+
twin->
|
2580
|
+
descriptor,
|
2581
|
+
nodeLength - index - 1);
|
2582
|
+
#else
|
2583
|
+
writeNucleotideInDescriptor(THYMINE,
|
2584
|
+
twin->
|
2585
|
+
descriptor,
|
2586
|
+
nodeLength - index - 1);
|
2587
|
+
#endif
|
2588
|
+
}
|
2589
|
+
}
|
2590
|
+
|
2591
|
+
index++;
|
2592
|
+
}
|
2593
|
+
|
2594
|
+
if (fgets(line, maxline, file) == NULL) {
|
2595
|
+
finished = true;
|
2596
|
+
}
|
2597
|
+
}
|
2598
|
+
|
2599
|
+
// Read arcs
|
2600
|
+
while (!finished && line[0] == 'A') {
|
2601
|
+
sscanf(line, "ARC\t%ld\t%ld\t%ld\n", &long_var,
|
2602
|
+
&long_var2, &long_var3);
|
2603
|
+
originID = (IDnum) long_var;
|
2604
|
+
destinationID = (IDnum) long_var2;
|
2605
|
+
multiplicity = (IDnum) long_var3;
|
2606
|
+
arc =
|
2607
|
+
createArc(getNodeInGraph(graph, originID),
|
2608
|
+
getNodeInGraph(graph, destinationID), graph);
|
2609
|
+
setMultiplicity(arc, multiplicity);
|
2610
|
+
if (fgets(line, maxline, file) == NULL)
|
2611
|
+
finished = true;
|
2612
|
+
}
|
2613
|
+
|
2614
|
+
fclose(file);
|
2615
|
+
return graph;
|
2616
|
+
}
|
2617
|
+
|
2618
|
+
// Prints out the information relative to the topology of a node into a new file
|
2619
|
+
// Internal to exportDOTGraph()
|
2620
|
+
void DOTNode(Node * node, FILE * outfile)
|
2621
|
+
{
|
2622
|
+
IDnum ID;
|
2623
|
+
Arc *arc;
|
2624
|
+
Node *otherNode;
|
2625
|
+
|
2626
|
+
ID = node->ID;
|
2627
|
+
if (ID < 0)
|
2628
|
+
return;
|
2629
|
+
|
2630
|
+
velvetFprintf(outfile, "\t%li [label=\"<left>|%li|<right>\"]\n", (long) ID, (long) ID);
|
2631
|
+
|
2632
|
+
for (arc = node->arc; arc != NULL; arc = arc->next) {
|
2633
|
+
otherNode = arc->destination;
|
2634
|
+
if (!(otherNode->ID >= ID || otherNode->ID <= -ID)) {
|
2635
|
+
continue;
|
2636
|
+
}
|
2637
|
+
|
2638
|
+
if (otherNode->ID > 0)
|
2639
|
+
velvetFprintf(outfile, "\t%li:right -> %li:left\n", (long) ID,
|
2640
|
+
(long) otherNode->ID);
|
2641
|
+
else
|
2642
|
+
velvetFprintf(outfile, "\t%li:right -> %li:right\n", (long) ID,
|
2643
|
+
(long) -otherNode->ID);
|
2644
|
+
}
|
2645
|
+
|
2646
|
+
for (arc = node->twinNode->arc; arc != NULL; arc = arc->next) {
|
2647
|
+
otherNode = arc->destination;
|
2648
|
+
if (!(otherNode->ID >= ID || otherNode->ID <= -ID)) {
|
2649
|
+
continue;
|
2650
|
+
}
|
2651
|
+
|
2652
|
+
if (otherNode->ID > 0)
|
2653
|
+
velvetFprintf(outfile, "\t%li:left -> %li:left\n", (long) ID,
|
2654
|
+
(long) otherNode->ID);
|
2655
|
+
else
|
2656
|
+
velvetFprintf(outfile, "\t%li:left -> %li:right\n", (long) ID,
|
2657
|
+
(long) -otherNode->ID);
|
2658
|
+
}
|
2659
|
+
}
|
2660
|
+
|
2661
|
+
TightString *expandNode(Node * node, int WORDLENGTH)
|
2662
|
+
{
|
2663
|
+
Nucleotide nucleotide;
|
2664
|
+
Coordinate index;
|
2665
|
+
TightString *tString =
|
2666
|
+
newTightString(node->length + WORDLENGTH - 1);
|
2667
|
+
Node *twin = node->twinNode;
|
2668
|
+
Coordinate length = node->length;
|
2669
|
+
|
2670
|
+
for (index = 0; index < WORDLENGTH; index++) {
|
2671
|
+
nucleotide =
|
2672
|
+
getNucleotideInDescriptor(twin->descriptor,
|
2673
|
+
length - index - 1);
|
2674
|
+
#ifndef COLOR
|
2675
|
+
writeNucleotideAtPosition(3 - nucleotide, index, tString);
|
2676
|
+
#else
|
2677
|
+
writeNucleotideAtPosition(nucleotide, index, tString);
|
2678
|
+
#endif
|
2679
|
+
}
|
2680
|
+
|
2681
|
+
for (index = 1; index < node->length; index++) {
|
2682
|
+
nucleotide =
|
2683
|
+
getNucleotideInDescriptor(node->descriptor, index);
|
2684
|
+
writeNucleotideAtPosition(nucleotide,
|
2685
|
+
index + WORDLENGTH - 1, tString);
|
2686
|
+
}
|
2687
|
+
|
2688
|
+
return tString;
|
2689
|
+
}
|
2690
|
+
|
2691
|
+
char *expandNodeFragment(Node * node, Coordinate contigStart,
|
2692
|
+
Coordinate contigFinish, int wordLength)
|
2693
|
+
{
|
2694
|
+
Nucleotide nucleotide;
|
2695
|
+
Coordinate index;
|
2696
|
+
Node *twin = node->twinNode;
|
2697
|
+
Coordinate length = contigFinish - contigStart;
|
2698
|
+
int wordShift = wordLength - 1;
|
2699
|
+
char *string;
|
2700
|
+
|
2701
|
+
if (length >= wordShift) {
|
2702
|
+
string = callocOrExit(length + wordLength, char);
|
2703
|
+
|
2704
|
+
for (index = 0; index < wordShift; index++) {
|
2705
|
+
nucleotide =
|
2706
|
+
getNucleotideInDescriptor(twin->descriptor,
|
2707
|
+
twin->length - contigStart -
|
2708
|
+
index - 1);
|
2709
|
+
#ifndef COLOR
|
2710
|
+
nucleotide = 3 - nucleotide;
|
2711
|
+
#endif
|
2712
|
+
|
2713
|
+
switch (nucleotide) {
|
2714
|
+
case ADENINE:
|
2715
|
+
string[index] = 'A';
|
2716
|
+
break;
|
2717
|
+
case CYTOSINE:
|
2718
|
+
string[index] = 'C';
|
2719
|
+
break;
|
2720
|
+
case GUANINE:
|
2721
|
+
string[index] = 'G';
|
2722
|
+
break;
|
2723
|
+
case THYMINE:
|
2724
|
+
string[index] = 'T';
|
2725
|
+
break;
|
2726
|
+
}
|
2727
|
+
|
2728
|
+
}
|
2729
|
+
|
2730
|
+
for (index = 0; index < length; index++) {
|
2731
|
+
nucleotide =
|
2732
|
+
getNucleotideInDescriptor(node->descriptor,
|
2733
|
+
contigStart + index);
|
2734
|
+
switch (nucleotide) {
|
2735
|
+
case ADENINE:
|
2736
|
+
string[index + wordShift] = 'A';
|
2737
|
+
break;
|
2738
|
+
case CYTOSINE:
|
2739
|
+
string[index + wordShift] = 'C';
|
2740
|
+
break;
|
2741
|
+
case GUANINE:
|
2742
|
+
string[index + wordShift] = 'G';
|
2743
|
+
break;
|
2744
|
+
case THYMINE:
|
2745
|
+
string[index + wordShift] = 'T';
|
2746
|
+
break;
|
2747
|
+
}
|
2748
|
+
}
|
2749
|
+
|
2750
|
+
string[length + wordShift] = '\0';
|
2751
|
+
} else {
|
2752
|
+
string = callocOrExit(length + 1, char);
|
2753
|
+
|
2754
|
+
for (index = 0; index < length; index++) {
|
2755
|
+
nucleotide =
|
2756
|
+
getNucleotideInDescriptor(node->descriptor, contigStart + index);
|
2757
|
+
switch (nucleotide) {
|
2758
|
+
case ADENINE:
|
2759
|
+
string[index] = 'A';
|
2760
|
+
break;
|
2761
|
+
case CYTOSINE:
|
2762
|
+
string[index] = 'C';
|
2763
|
+
break;
|
2764
|
+
case GUANINE:
|
2765
|
+
string[index] = 'G';
|
2766
|
+
break;
|
2767
|
+
case THYMINE:
|
2768
|
+
string[index] = 'T';
|
2769
|
+
break;
|
2770
|
+
}
|
2771
|
+
}
|
2772
|
+
|
2773
|
+
string[length] = '\0';
|
2774
|
+
}
|
2775
|
+
|
2776
|
+
return string;
|
2777
|
+
}
|
2778
|
+
|
2779
|
+
boolean readStartsAreActivated(Graph * graph)
|
2780
|
+
{
|
2781
|
+
return graph->nodeReads != NULL;
|
2782
|
+
}
|
2783
|
+
|
2784
|
+
void activateReadStarts(Graph * graph)
|
2785
|
+
{
|
2786
|
+
graph->nodeReads =
|
2787
|
+
callocOrExit(2 * graph->nodeCount + 1, ShortReadMarker *);
|
2788
|
+
graph->nodeReadCounts =
|
2789
|
+
callocOrExit(2 * graph->nodeCount + 1, IDnum);
|
2790
|
+
}
|
2791
|
+
|
2792
|
+
void deactivateReadStarts(Graph * graph)
|
2793
|
+
{
|
2794
|
+
free(graph->nodeReads);
|
2795
|
+
free(graph->nodeReadCounts);
|
2796
|
+
|
2797
|
+
graph->nodeReads = NULL;
|
2798
|
+
graph->nodeReadCounts = NULL;
|
2799
|
+
}
|
2800
|
+
|
2801
|
+
boolean findIDnumInArray(IDnum query, IDnum * array, IDnum arrayLength)
|
2802
|
+
{
|
2803
|
+
IDnum leftIndex = 0;
|
2804
|
+
IDnum rightIndex = arrayLength;
|
2805
|
+
IDnum middleIndex;
|
2806
|
+
|
2807
|
+
if (arrayLength == 0)
|
2808
|
+
return false;
|
2809
|
+
|
2810
|
+
while (true) {
|
2811
|
+
middleIndex = leftIndex + (rightIndex - leftIndex) / 2;
|
2812
|
+
|
2813
|
+
if (array[middleIndex] == query)
|
2814
|
+
return true;
|
2815
|
+
else if (leftIndex >= rightIndex)
|
2816
|
+
return false;
|
2817
|
+
else if (array[middleIndex] > query)
|
2818
|
+
rightIndex = middleIndex;
|
2819
|
+
else if (leftIndex == middleIndex)
|
2820
|
+
leftIndex++;
|
2821
|
+
else
|
2822
|
+
leftIndex = middleIndex;
|
2823
|
+
}
|
2824
|
+
}
|
2825
|
+
|
2826
|
+
static inline int compareShortReadMarkers(const void *A, const void *B)
|
2827
|
+
{
|
2828
|
+
IDnum a = ((ShortReadMarker *) A)->readID;
|
2829
|
+
IDnum b = ((ShortReadMarker *) B)->readID;
|
2830
|
+
|
2831
|
+
if (a > b)
|
2832
|
+
return 1;
|
2833
|
+
if (a == b)
|
2834
|
+
return 0;
|
2835
|
+
return -1;
|
2836
|
+
}
|
2837
|
+
|
2838
|
+
static inline int compareIDnums(const void *A, const void *B)
|
2839
|
+
{
|
2840
|
+
IDnum a = *((IDnum *) A);
|
2841
|
+
IDnum b = *((IDnum *) B);
|
2842
|
+
|
2843
|
+
if (a > b)
|
2844
|
+
return 1;
|
2845
|
+
if (a == b)
|
2846
|
+
return 0;
|
2847
|
+
return -1;
|
2848
|
+
}
|
2849
|
+
|
2850
|
+
void incrementReadStartCount(Node * node, Graph * graph)
|
2851
|
+
{
|
2852
|
+
graph->nodeReadCounts[node->ID + graph->nodeCount]++;
|
2853
|
+
}
|
2854
|
+
|
2855
|
+
void createNodeReadStartArrays(Graph * graph)
|
2856
|
+
{
|
2857
|
+
IDnum index;
|
2858
|
+
|
2859
|
+
if (graph->nodeReads == NULL)
|
2860
|
+
return;
|
2861
|
+
|
2862
|
+
for (index = 0; index <= 2 * (graph->nodeCount); index++) {
|
2863
|
+
if (graph->nodeReadCounts[index] != 0) {
|
2864
|
+
graph->nodeReads[index] =
|
2865
|
+
mallocOrExit(graph->nodeReadCounts[index],
|
2866
|
+
ShortReadMarker);
|
2867
|
+
graph->nodeReadCounts[index] = 0;
|
2868
|
+
} else {
|
2869
|
+
graph->nodeReads[index] = NULL;
|
2870
|
+
}
|
2871
|
+
}
|
2872
|
+
}
|
2873
|
+
|
2874
|
+
void orderNodeReadStartArrays(Graph * graph)
|
2875
|
+
{
|
2876
|
+
IDnum index;
|
2877
|
+
|
2878
|
+
if (graph->nodeReads == NULL)
|
2879
|
+
return;
|
2880
|
+
|
2881
|
+
for (index = 0; index <= 2 * (graph->nodeCount); index++)
|
2882
|
+
if (graph->nodeReadCounts[index] != 0)
|
2883
|
+
qsort(graph->nodeReads[index],
|
2884
|
+
graph->nodeReadCounts[index],
|
2885
|
+
sizeof(ShortReadMarker),
|
2886
|
+
compareShortReadMarkers);
|
2887
|
+
}
|
2888
|
+
|
2889
|
+
void addReadStart(Node * node, IDnum seqID, Coordinate position,
|
2890
|
+
Graph * graph, Coordinate offset)
|
2891
|
+
{
|
2892
|
+
IDnum nodeIndex = getNodeID(node) + graph->nodeCount;
|
2893
|
+
|
2894
|
+
ShortReadMarker *array = graph->nodeReads[nodeIndex];
|
2895
|
+
IDnum arrayLength = graph->nodeReadCounts[nodeIndex];
|
2896
|
+
|
2897
|
+
array[arrayLength].readID = seqID;
|
2898
|
+
array[arrayLength].position = position;
|
2899
|
+
array[arrayLength].offset = (ShortLength) offset;
|
2900
|
+
// printf("node %d, seq %d, pos %ld, offset %ld\n", getNodeID(node), seqID, position, offset);
|
2901
|
+
graph->nodeReadCounts[nodeIndex]++;
|
2902
|
+
}
|
2903
|
+
|
2904
|
+
void blurLastShortReadMarker(Node * node, Graph * graph)
|
2905
|
+
{
|
2906
|
+
IDnum nodeIndex = getNodeID(node) + nodeCount(graph);
|
2907
|
+
IDnum index = graph->nodeReadCounts[nodeIndex] - 1;
|
2908
|
+
ShortReadMarker *marker;
|
2909
|
+
|
2910
|
+
if (index >= 0)
|
2911
|
+
marker = &(graph->nodeReads[nodeIndex][index]);
|
2912
|
+
else
|
2913
|
+
abort();
|
2914
|
+
|
2915
|
+
setShortReadMarkerPosition(marker, -1);
|
2916
|
+
}
|
2917
|
+
|
2918
|
+
ShortReadMarker *commonNodeReads(Node * nodeA, Node * nodeB, Graph * graph,
|
2919
|
+
IDnum * length)
|
2920
|
+
{
|
2921
|
+
IDnum targetID, targetLength, targetIndex, targetVal;
|
2922
|
+
IDnum sourceID, sourceLength, sourceIndex, sourceVal;
|
2923
|
+
IDnum mergeLength;
|
2924
|
+
ShortReadMarker *mergeArray, *targetArray, *sourceArray;
|
2925
|
+
|
2926
|
+
if (graph->nodeReads == NULL) {
|
2927
|
+
*length = 0;
|
2928
|
+
return NULL;
|
2929
|
+
}
|
2930
|
+
|
2931
|
+
if (nodeA == NULL || nodeB == NULL) {
|
2932
|
+
*length = 0;
|
2933
|
+
return NULL;
|
2934
|
+
}
|
2935
|
+
|
2936
|
+
targetID = getNodeID(nodeA) + graph->nodeCount;
|
2937
|
+
targetArray = graph->nodeReads[targetID];
|
2938
|
+
targetLength = graph->nodeReadCounts[targetID];
|
2939
|
+
|
2940
|
+
sourceID = getNodeID(nodeB) + graph->nodeCount;
|
2941
|
+
sourceArray = graph->nodeReads[sourceID];
|
2942
|
+
sourceLength = graph->nodeReadCounts[sourceID];
|
2943
|
+
|
2944
|
+
if (sourceArray == NULL || targetArray == NULL) {
|
2945
|
+
*length = 0;
|
2946
|
+
return NULL;
|
2947
|
+
}
|
2948
|
+
|
2949
|
+
mergeArray =
|
2950
|
+
mallocOrExit(sourceLength +
|
2951
|
+
targetLength, ShortReadMarker);
|
2952
|
+
|
2953
|
+
mergeLength = 0;
|
2954
|
+
sourceIndex = 0;
|
2955
|
+
targetIndex = 0;
|
2956
|
+
sourceVal = sourceArray[0].readID;
|
2957
|
+
targetVal = targetArray[0].readID;
|
2958
|
+
|
2959
|
+
while (sourceIndex < sourceLength && targetIndex < targetLength) {
|
2960
|
+
switch (compareIDnums(&sourceVal, &targetVal)) {
|
2961
|
+
case -1:
|
2962
|
+
mergeArray[mergeLength].readID = sourceVal;
|
2963
|
+
mergeArray[mergeLength].position = -1;
|
2964
|
+
mergeArray[mergeLength].offset = -1;
|
2965
|
+
mergeLength++;
|
2966
|
+
sourceIndex++;
|
2967
|
+
if (sourceIndex < sourceLength)
|
2968
|
+
sourceVal =
|
2969
|
+
sourceArray[sourceIndex].readID;
|
2970
|
+
break;
|
2971
|
+
case 0:
|
2972
|
+
mergeArray[mergeLength].readID = sourceVal;
|
2973
|
+
mergeArray[mergeLength].position = -1;
|
2974
|
+
mergeArray[mergeLength].offset = -1;
|
2975
|
+
mergeLength++;
|
2976
|
+
sourceIndex++;
|
2977
|
+
if (sourceIndex < sourceLength)
|
2978
|
+
sourceVal =
|
2979
|
+
sourceArray[sourceIndex].readID;
|
2980
|
+
targetIndex++;
|
2981
|
+
if (targetIndex < targetLength)
|
2982
|
+
targetVal =
|
2983
|
+
targetArray[targetIndex].readID;
|
2984
|
+
break;
|
2985
|
+
case 1:
|
2986
|
+
mergeArray[mergeLength].readID = targetVal;
|
2987
|
+
mergeArray[mergeLength].position = -1;
|
2988
|
+
mergeArray[mergeLength].offset = -1;
|
2989
|
+
mergeLength++;
|
2990
|
+
targetIndex++;
|
2991
|
+
if (targetIndex < targetLength)
|
2992
|
+
targetVal =
|
2993
|
+
targetArray[targetIndex].readID;
|
2994
|
+
}
|
2995
|
+
}
|
2996
|
+
|
2997
|
+
while (sourceIndex < sourceLength) {
|
2998
|
+
mergeArray[mergeLength].readID =
|
2999
|
+
sourceArray[sourceIndex].readID;
|
3000
|
+
mergeArray[mergeLength].position = -1;
|
3001
|
+
mergeArray[mergeLength].offset = -1;
|
3002
|
+
mergeLength++;
|
3003
|
+
sourceIndex++;
|
3004
|
+
}
|
3005
|
+
|
3006
|
+
while (targetIndex < targetLength) {
|
3007
|
+
mergeArray[mergeLength].readID =
|
3008
|
+
targetArray[targetIndex].readID;
|
3009
|
+
mergeArray[mergeLength].position = -1;
|
3010
|
+
mergeArray[mergeLength].offset = -1;
|
3011
|
+
mergeLength++;
|
3012
|
+
targetIndex++;
|
3013
|
+
}
|
3014
|
+
|
3015
|
+
*length = mergeLength;
|
3016
|
+
return mergeArray;
|
3017
|
+
}
|
3018
|
+
|
3019
|
+
ShortReadMarker *extractFrontOfNodeReads(Node * node,
|
3020
|
+
Coordinate breakpoint,
|
3021
|
+
Graph * graph, IDnum * length,
|
3022
|
+
PassageMarkerI sourceMarker,
|
3023
|
+
ShortLength * lengths)
|
3024
|
+
{
|
3025
|
+
IDnum sourceID;
|
3026
|
+
IDnum mergeLength, newLength, sourceLength;
|
3027
|
+
IDnum sourceIndex;
|
3028
|
+
ShortReadMarker *mergeArray, *sourceArray, *newArray;
|
3029
|
+
ShortReadMarker *mergePtr, *sourcePtr, *newPtr;
|
3030
|
+
Coordinate finish;
|
3031
|
+
Coordinate revBreakpoint;
|
3032
|
+
|
3033
|
+
if (graph->nodeReads == NULL) {
|
3034
|
+
*length = 0;
|
3035
|
+
return NULL;
|
3036
|
+
}
|
3037
|
+
|
3038
|
+
if (node == NULL) {
|
3039
|
+
*length = 0;
|
3040
|
+
return NULL;
|
3041
|
+
}
|
3042
|
+
|
3043
|
+
if (breakpoint == 0) {
|
3044
|
+
return commonNodeReads(node,
|
3045
|
+
getTwinNode(getNode
|
3046
|
+
(getPreviousInSequence
|
3047
|
+
(sourceMarker))),
|
3048
|
+
graph, length);
|
3049
|
+
}
|
3050
|
+
|
3051
|
+
sourceID = getNodeID(node) + graph->nodeCount;
|
3052
|
+
sourceArray = graph->nodeReads[sourceID];
|
3053
|
+
sourceLength = graph->nodeReadCounts[sourceID];
|
3054
|
+
|
3055
|
+
if (sourceArray == NULL) {
|
3056
|
+
*length = 0;
|
3057
|
+
return NULL;
|
3058
|
+
}
|
3059
|
+
|
3060
|
+
revBreakpoint = node->length - breakpoint;
|
3061
|
+
|
3062
|
+
mergeLength = 0;
|
3063
|
+
newLength = 0;
|
3064
|
+
sourcePtr = sourceArray;
|
3065
|
+
for (sourceIndex = 0; sourceIndex < sourceLength; sourceIndex++) {
|
3066
|
+
if (sourcePtr->position == -1) {
|
3067
|
+
newLength++;
|
3068
|
+
mergeLength++;
|
3069
|
+
} else {
|
3070
|
+
finish =
|
3071
|
+
sourcePtr->position - sourcePtr->offset +
|
3072
|
+
lengths[sourcePtr->readID - 1];
|
3073
|
+
if (sourcePtr->position < revBreakpoint)
|
3074
|
+
newLength++;
|
3075
|
+
if (finish > revBreakpoint)
|
3076
|
+
mergeLength++;
|
3077
|
+
}
|
3078
|
+
sourcePtr++;
|
3079
|
+
}
|
3080
|
+
|
3081
|
+
newArray = mallocOrExit(newLength, ShortReadMarker);
|
3082
|
+
mergeArray = mallocOrExit(mergeLength, ShortReadMarker);
|
3083
|
+
|
3084
|
+
mergePtr = mergeArray;
|
3085
|
+
newPtr = newArray;
|
3086
|
+
sourcePtr = sourceArray;
|
3087
|
+
mergeLength = 0;
|
3088
|
+
newLength = 0;
|
3089
|
+
for (sourceIndex = 0; sourceIndex < sourceLength; sourceIndex++) {
|
3090
|
+
if (sourcePtr->position == -1) {
|
3091
|
+
mergePtr->readID = sourcePtr->readID;
|
3092
|
+
setShortReadMarkerPosition(mergePtr, -1);
|
3093
|
+
setShortReadMarkerOffset(mergePtr, -1);
|
3094
|
+
mergePtr++;
|
3095
|
+
mergeLength++;
|
3096
|
+
newPtr->readID = sourcePtr->readID;
|
3097
|
+
setShortReadMarkerPosition(newPtr, -1);
|
3098
|
+
setShortReadMarkerOffset(newPtr, -1);
|
3099
|
+
newPtr++;
|
3100
|
+
newLength++;
|
3101
|
+
} else {
|
3102
|
+
finish =
|
3103
|
+
sourcePtr->position - sourcePtr->offset +
|
3104
|
+
lengths[sourcePtr->readID - 1];
|
3105
|
+
if (sourcePtr->position < revBreakpoint) {
|
3106
|
+
newPtr->readID = sourcePtr->readID;
|
3107
|
+
setShortReadMarkerPosition(newPtr,
|
3108
|
+
sourcePtr->
|
3109
|
+
position);
|
3110
|
+
setShortReadMarkerOffset(newPtr,
|
3111
|
+
sourcePtr->
|
3112
|
+
offset);
|
3113
|
+
newPtr++;
|
3114
|
+
newLength++;
|
3115
|
+
|
3116
|
+
// Saddle back reads:
|
3117
|
+
if (finish > revBreakpoint) {
|
3118
|
+
mergePtr->readID =
|
3119
|
+
sourcePtr->readID;
|
3120
|
+
setShortReadMarkerPosition
|
3121
|
+
(mergePtr, 0);
|
3122
|
+
setShortReadMarkerOffset(mergePtr,
|
3123
|
+
sourcePtr->
|
3124
|
+
offset +
|
3125
|
+
revBreakpoint
|
3126
|
+
-
|
3127
|
+
sourcePtr->
|
3128
|
+
position);
|
3129
|
+
mergePtr++;
|
3130
|
+
}
|
3131
|
+
} else if (finish > revBreakpoint) {
|
3132
|
+
mergePtr->readID = sourcePtr->readID;
|
3133
|
+
setShortReadMarkerPosition(mergePtr,
|
3134
|
+
sourcePtr->
|
3135
|
+
position - revBreakpoint);
|
3136
|
+
setShortReadMarkerOffset(mergePtr,
|
3137
|
+
sourcePtr->
|
3138
|
+
offset);
|
3139
|
+
mergePtr++;
|
3140
|
+
mergeLength++;
|
3141
|
+
}
|
3142
|
+
}
|
3143
|
+
|
3144
|
+
sourcePtr++;
|
3145
|
+
}
|
3146
|
+
|
3147
|
+
free(sourceArray);
|
3148
|
+
graph->nodeReads[sourceID] = newArray;
|
3149
|
+
graph->nodeReadCounts[sourceID] = newLength;
|
3150
|
+
|
3151
|
+
*length = mergeLength;
|
3152
|
+
return mergeArray;
|
3153
|
+
}
|
3154
|
+
|
3155
|
+
ShortReadMarker *extractBackOfNodeReads(Node * node, Coordinate breakpoint,
|
3156
|
+
Graph * graph, IDnum * length,
|
3157
|
+
PassageMarkerI sourceMarker,
|
3158
|
+
ShortLength * lengths)
|
3159
|
+
{
|
3160
|
+
IDnum sourceID;
|
3161
|
+
IDnum mergeLength, newLength, sourceLength;
|
3162
|
+
IDnum sourceIndex;
|
3163
|
+
ShortReadMarker *mergeArray, *sourceArray, *newArray;
|
3164
|
+
ShortReadMarker *mergePtr, *sourcePtr, *newPtr;
|
3165
|
+
Coordinate finish;
|
3166
|
+
|
3167
|
+
if (graph->nodeReads == NULL) {
|
3168
|
+
*length = 0;
|
3169
|
+
return NULL;
|
3170
|
+
}
|
3171
|
+
|
3172
|
+
if (node == NULL) {
|
3173
|
+
*length = 0;
|
3174
|
+
return NULL;
|
3175
|
+
}
|
3176
|
+
|
3177
|
+
if (breakpoint == 0) {
|
3178
|
+
return
|
3179
|
+
commonNodeReads(getNode
|
3180
|
+
(getPreviousInSequence(sourceMarker)),
|
3181
|
+
node, graph, length);
|
3182
|
+
}
|
3183
|
+
|
3184
|
+
sourceID = getNodeID(node) + graph->nodeCount;
|
3185
|
+
sourceArray = graph->nodeReads[sourceID];
|
3186
|
+
sourceLength = graph->nodeReadCounts[sourceID];
|
3187
|
+
|
3188
|
+
if (sourceArray == NULL) {
|
3189
|
+
*length = 0;
|
3190
|
+
return NULL;
|
3191
|
+
}
|
3192
|
+
|
3193
|
+
mergeLength = 0;
|
3194
|
+
newLength = 0;
|
3195
|
+
sourcePtr = sourceArray;
|
3196
|
+
for (sourceIndex = 0; sourceIndex < sourceLength; sourceIndex++) {
|
3197
|
+
if (sourcePtr->position == -1) {
|
3198
|
+
mergeLength++;
|
3199
|
+
newLength++;
|
3200
|
+
} else {
|
3201
|
+
finish =
|
3202
|
+
sourcePtr->position - sourcePtr->offset +
|
3203
|
+
lengths[sourcePtr->readID - 1];
|
3204
|
+
if (sourcePtr->position < breakpoint)
|
3205
|
+
mergeLength++;
|
3206
|
+
if (finish > breakpoint)
|
3207
|
+
newLength++;
|
3208
|
+
}
|
3209
|
+
sourcePtr++;
|
3210
|
+
}
|
3211
|
+
|
3212
|
+
newArray = mallocOrExit(newLength, ShortReadMarker);
|
3213
|
+
mergeArray = mallocOrExit(mergeLength, ShortReadMarker);
|
3214
|
+
|
3215
|
+
mergePtr = mergeArray;
|
3216
|
+
newPtr = newArray;
|
3217
|
+
sourcePtr = sourceArray;
|
3218
|
+
for (sourceIndex = 0; sourceIndex < sourceLength; sourceIndex++) {
|
3219
|
+
if (sourcePtr->position == -1) {
|
3220
|
+
mergePtr->readID = sourcePtr->readID;
|
3221
|
+
setShortReadMarkerPosition(mergePtr, -1);
|
3222
|
+
setShortReadMarkerOffset(mergePtr, -1);
|
3223
|
+
mergePtr++;
|
3224
|
+
|
3225
|
+
newPtr->readID = sourcePtr->readID;
|
3226
|
+
setShortReadMarkerPosition(newPtr, -1);
|
3227
|
+
setShortReadMarkerOffset(newPtr, -1);
|
3228
|
+
newPtr++;
|
3229
|
+
|
3230
|
+
sourcePtr++;
|
3231
|
+
continue;
|
3232
|
+
} else {
|
3233
|
+
finish =
|
3234
|
+
sourcePtr->position - sourcePtr->offset +
|
3235
|
+
lengths[sourcePtr->readID - 1];
|
3236
|
+
|
3237
|
+
if (sourcePtr->position < breakpoint) {
|
3238
|
+
mergePtr->readID = sourcePtr->readID;
|
3239
|
+
setShortReadMarkerPosition(mergePtr,
|
3240
|
+
sourcePtr->
|
3241
|
+
position);
|
3242
|
+
setShortReadMarkerOffset(mergePtr,
|
3243
|
+
sourcePtr->
|
3244
|
+
offset);
|
3245
|
+
mergePtr++;
|
3246
|
+
|
3247
|
+
// Saddle back reads:
|
3248
|
+
if (finish > breakpoint) {
|
3249
|
+
newPtr->readID = sourcePtr->readID;
|
3250
|
+
setShortReadMarkerPosition(newPtr,
|
3251
|
+
0);
|
3252
|
+
setShortReadMarkerOffset(newPtr,
|
3253
|
+
sourcePtr->
|
3254
|
+
offset +
|
3255
|
+
breakpoint
|
3256
|
+
-
|
3257
|
+
sourcePtr->
|
3258
|
+
position);
|
3259
|
+
newPtr++;
|
3260
|
+
}
|
3261
|
+
} else if (finish > breakpoint) {
|
3262
|
+
newPtr->readID = sourcePtr->readID;
|
3263
|
+
setShortReadMarkerPosition(newPtr,
|
3264
|
+
sourcePtr->
|
3265
|
+
position -
|
3266
|
+
breakpoint);
|
3267
|
+
setShortReadMarkerOffset(newPtr,
|
3268
|
+
sourcePtr->
|
3269
|
+
offset);
|
3270
|
+
newPtr++;
|
3271
|
+
}
|
3272
|
+
}
|
3273
|
+
|
3274
|
+
sourcePtr++;
|
3275
|
+
}
|
3276
|
+
|
3277
|
+
free(sourceArray);
|
3278
|
+
graph->nodeReads[sourceID] = newArray;
|
3279
|
+
graph->nodeReadCounts[sourceID] = newLength;
|
3280
|
+
|
3281
|
+
*length = mergeLength;
|
3282
|
+
return mergeArray;
|
3283
|
+
}
|
3284
|
+
|
3285
|
+
void spreadReadIDs(ShortReadMarker * reads, IDnum readCount, Node * node,
|
3286
|
+
Graph * graph)
|
3287
|
+
{
|
3288
|
+
IDnum targetID, targetLength, targetIndex, targetVal;
|
3289
|
+
IDnum sourceLength, sourceIndex, sourceVal;
|
3290
|
+
IDnum mergeLength;
|
3291
|
+
ShortReadMarker *sourceArray, *targetArray, *mergeArray;
|
3292
|
+
ShortReadMarker *sourcePtr, *targetPtr, *mergePtr;
|
3293
|
+
Coordinate targetPosition;
|
3294
|
+
//ShortLength nodeLength = (ShortLength) getNodeLength(node);
|
3295
|
+
ShortLength targetOffset;
|
3296
|
+
|
3297
|
+
if (graph->nodeReads == NULL || reads == NULL || node == NULL)
|
3298
|
+
return;
|
3299
|
+
|
3300
|
+
targetID = getNodeID(node) + graph->nodeCount;
|
3301
|
+
targetArray = graph->nodeReads[targetID];
|
3302
|
+
targetLength = graph->nodeReadCounts[targetID];
|
3303
|
+
targetPtr = targetArray;
|
3304
|
+
|
3305
|
+
sourceArray = reads;
|
3306
|
+
sourceLength = readCount;
|
3307
|
+
sourcePtr = sourceArray;
|
3308
|
+
|
3309
|
+
if (targetArray == NULL) {
|
3310
|
+
mergeArray =
|
3311
|
+
mallocOrExit(sourceLength, ShortReadMarker);
|
3312
|
+
mergePtr = mergeArray;
|
3313
|
+
|
3314
|
+
sourceIndex = 0;
|
3315
|
+
while (sourceIndex < sourceLength) {
|
3316
|
+
mergePtr->readID = sourcePtr->readID;
|
3317
|
+
setShortReadMarkerPosition(mergePtr, -1);
|
3318
|
+
setShortReadMarkerOffset(mergePtr, -1);
|
3319
|
+
mergePtr++;
|
3320
|
+
sourcePtr++;
|
3321
|
+
sourceIndex++;
|
3322
|
+
}
|
3323
|
+
|
3324
|
+
graph->nodeReads[targetID] = mergeArray;
|
3325
|
+
graph->nodeReadCounts[targetID] = sourceLength;
|
3326
|
+
return;
|
3327
|
+
}
|
3328
|
+
|
3329
|
+
mergeArray =
|
3330
|
+
mallocOrExit(sourceLength +
|
3331
|
+
targetLength, ShortReadMarker);
|
3332
|
+
mergePtr = mergeArray;
|
3333
|
+
|
3334
|
+
mergeLength = 0;
|
3335
|
+
sourceIndex = 0;
|
3336
|
+
targetIndex = 0;
|
3337
|
+
sourceVal = sourcePtr->readID;
|
3338
|
+
targetVal = targetPtr->readID;
|
3339
|
+
targetPosition = targetPtr->position;
|
3340
|
+
targetOffset = targetPtr->offset;
|
3341
|
+
|
3342
|
+
while (sourceIndex < sourceLength && targetIndex < targetLength) {
|
3343
|
+
if (sourceVal < targetVal) {
|
3344
|
+
mergePtr->readID = sourceVal;
|
3345
|
+
setShortReadMarkerPosition(mergePtr, -1);
|
3346
|
+
setShortReadMarkerOffset(mergePtr, -1);
|
3347
|
+
sourceIndex++;
|
3348
|
+
sourcePtr++;
|
3349
|
+
if (sourceIndex < sourceLength)
|
3350
|
+
sourceVal = sourcePtr->readID;
|
3351
|
+
} else if (sourceVal == targetVal) {
|
3352
|
+
mergePtr->readID = sourceVal;
|
3353
|
+
setShortReadMarkerPosition(mergePtr, -1);
|
3354
|
+
setShortReadMarkerOffset(mergePtr, -1);
|
3355
|
+
sourceIndex++;
|
3356
|
+
sourcePtr++;
|
3357
|
+
if (sourceIndex < sourceLength)
|
3358
|
+
sourceVal = sourcePtr->readID;
|
3359
|
+
targetIndex++;
|
3360
|
+
targetPtr++;
|
3361
|
+
if (targetIndex < targetLength) {
|
3362
|
+
targetVal = targetPtr->readID;
|
3363
|
+
targetPosition = targetPtr->position;
|
3364
|
+
targetOffset = targetPtr->offset;
|
3365
|
+
}
|
3366
|
+
} else {
|
3367
|
+
mergePtr->readID = targetVal;
|
3368
|
+
setShortReadMarkerPosition(mergePtr,
|
3369
|
+
targetPosition);
|
3370
|
+
setShortReadMarkerOffset(mergePtr, targetOffset);
|
3371
|
+
targetIndex++;
|
3372
|
+
targetPtr++;
|
3373
|
+
if (targetIndex < targetLength) {
|
3374
|
+
targetVal = targetPtr->readID;
|
3375
|
+
targetPosition = targetPtr->position;
|
3376
|
+
targetOffset = targetPtr->offset;
|
3377
|
+
}
|
3378
|
+
}
|
3379
|
+
|
3380
|
+
mergeLength++;
|
3381
|
+
mergePtr++;
|
3382
|
+
}
|
3383
|
+
|
3384
|
+
while (sourceIndex < sourceLength) {
|
3385
|
+
mergePtr->readID = sourcePtr->readID;
|
3386
|
+
setShortReadMarkerPosition(mergePtr, -1);
|
3387
|
+
setShortReadMarkerOffset(mergePtr, -1);
|
3388
|
+
mergeLength++;
|
3389
|
+
mergePtr++;
|
3390
|
+
sourceIndex++;
|
3391
|
+
sourcePtr++;
|
3392
|
+
}
|
3393
|
+
|
3394
|
+
while (targetIndex < targetLength) {
|
3395
|
+
mergePtr->readID = targetPtr->readID;
|
3396
|
+
setShortReadMarkerPosition(mergePtr, targetPtr->position);
|
3397
|
+
setShortReadMarkerOffset(mergePtr, targetPtr->offset);
|
3398
|
+
mergeLength++;
|
3399
|
+
mergePtr++;
|
3400
|
+
targetIndex++;
|
3401
|
+
targetPtr++;
|
3402
|
+
}
|
3403
|
+
|
3404
|
+
free(targetArray);
|
3405
|
+
graph->nodeReads[targetID] = mergeArray;
|
3406
|
+
graph->nodeReadCounts[targetID] = mergeLength;
|
3407
|
+
}
|
3408
|
+
|
3409
|
+
static inline Coordinate min(Coordinate A, Coordinate B)
|
3410
|
+
{
|
3411
|
+
return A < B ? A : B;
|
3412
|
+
}
|
3413
|
+
|
3414
|
+
static inline ShortLength min_short(ShortLength A, ShortLength B)
|
3415
|
+
{
|
3416
|
+
return A < B ? A : B;
|
3417
|
+
}
|
3418
|
+
|
3419
|
+
void injectShortReads(ShortReadMarker * sourceArray, IDnum sourceLength,
|
3420
|
+
Node * target, Graph * graph)
|
3421
|
+
{
|
3422
|
+
IDnum targetID = getNodeID(target) + graph->nodeCount;
|
3423
|
+
ShortReadMarker *targetArray = graph->nodeReads[targetID];
|
3424
|
+
IDnum targetLength = graph->nodeReadCounts[targetID];
|
3425
|
+
ShortReadMarker *targetPtr = targetArray;
|
3426
|
+
ShortReadMarker *sourcePtr = sourceArray;
|
3427
|
+
ShortReadMarker *mergeArray, *mergePtr;
|
3428
|
+
IDnum mergeLength;
|
3429
|
+
Coordinate targetPosition, sourcePosition;
|
3430
|
+
ShortLength targetOffset, sourceOffset;
|
3431
|
+
IDnum targetIndex, targetVal, sourceIndex, sourceVal;
|
3432
|
+
|
3433
|
+
if (sourceLength == 0) {
|
3434
|
+
free(sourceArray);
|
3435
|
+
return;
|
3436
|
+
}
|
3437
|
+
|
3438
|
+
if (targetLength == 0) {
|
3439
|
+
free(targetArray);
|
3440
|
+
graph->nodeReads[targetID] = sourceArray;
|
3441
|
+
graph->nodeReadCounts[targetID] = sourceLength;
|
3442
|
+
return;
|
3443
|
+
}
|
3444
|
+
|
3445
|
+
mergeArray =
|
3446
|
+
mallocOrExit(sourceLength +
|
3447
|
+
targetLength, ShortReadMarker);
|
3448
|
+
mergePtr = mergeArray;
|
3449
|
+
|
3450
|
+
mergeLength = 0;
|
3451
|
+
sourceIndex = 0;
|
3452
|
+
targetIndex = 0;
|
3453
|
+
targetVal = targetPtr->readID;
|
3454
|
+
targetPosition = targetPtr->position;
|
3455
|
+
targetOffset = targetPtr->offset;
|
3456
|
+
sourceVal = sourcePtr->readID;
|
3457
|
+
sourcePosition = sourcePtr->position;
|
3458
|
+
sourceOffset = sourcePtr->offset;
|
3459
|
+
|
3460
|
+
while (sourceIndex < sourceLength && targetIndex < targetLength) {
|
3461
|
+
if (sourceVal < targetVal) {
|
3462
|
+
mergePtr->readID = sourceVal;
|
3463
|
+
setShortReadMarkerPosition(mergePtr,
|
3464
|
+
sourcePosition);
|
3465
|
+
setShortReadMarkerOffset(mergePtr, sourceOffset);
|
3466
|
+
sourceIndex++;
|
3467
|
+
if (sourceIndex < sourceLength) {
|
3468
|
+
sourcePtr++;
|
3469
|
+
sourceVal = sourcePtr->readID;
|
3470
|
+
sourcePosition = sourcePtr->position;
|
3471
|
+
sourceOffset = sourcePtr->offset;
|
3472
|
+
}
|
3473
|
+
} else if (sourceVal == targetVal) {
|
3474
|
+
mergePtr->readID = sourceVal;
|
3475
|
+
if (sourcePosition == -1 && targetPosition == -1) {
|
3476
|
+
setShortReadMarkerPosition(mergePtr, -1);
|
3477
|
+
setShortReadMarkerOffset(mergePtr, -1);
|
3478
|
+
} else if (sourcePosition == -1) {
|
3479
|
+
setShortReadMarkerPosition(mergePtr,
|
3480
|
+
targetPosition);
|
3481
|
+
setShortReadMarkerOffset(mergePtr,
|
3482
|
+
targetOffset);
|
3483
|
+
} else if (targetPosition == -1) {
|
3484
|
+
setShortReadMarkerPosition(mergePtr,
|
3485
|
+
sourcePosition);
|
3486
|
+
setShortReadMarkerOffset(mergePtr,
|
3487
|
+
sourceOffset);
|
3488
|
+
} else {
|
3489
|
+
setShortReadMarkerPosition(mergePtr,
|
3490
|
+
min
|
3491
|
+
(sourcePosition,
|
3492
|
+
targetPosition));
|
3493
|
+
setShortReadMarkerOffset(mergePtr,
|
3494
|
+
min_short
|
3495
|
+
(sourceOffset,
|
3496
|
+
targetOffset));
|
3497
|
+
}
|
3498
|
+
sourceIndex++;
|
3499
|
+
if (sourceIndex < sourceLength) {
|
3500
|
+
sourcePtr++;
|
3501
|
+
sourceVal = sourcePtr->readID;
|
3502
|
+
sourcePosition = sourcePtr->position;
|
3503
|
+
sourceOffset = sourcePtr->offset;
|
3504
|
+
}
|
3505
|
+
targetIndex++;
|
3506
|
+
if (targetIndex < targetLength) {
|
3507
|
+
targetPtr++;
|
3508
|
+
targetVal = targetPtr->readID;
|
3509
|
+
targetPosition = targetPtr->position;
|
3510
|
+
targetOffset = targetPtr->offset;
|
3511
|
+
}
|
3512
|
+
} else {
|
3513
|
+
mergePtr->readID = targetVal;
|
3514
|
+
setShortReadMarkerPosition(mergePtr,
|
3515
|
+
targetPosition);
|
3516
|
+
setShortReadMarkerOffset(mergePtr, targetOffset);
|
3517
|
+
targetIndex++;
|
3518
|
+
if (targetIndex < targetLength) {
|
3519
|
+
targetPtr++;
|
3520
|
+
targetVal = targetPtr->readID;
|
3521
|
+
targetPosition = targetPtr->position;
|
3522
|
+
targetOffset = targetPtr->offset;
|
3523
|
+
}
|
3524
|
+
}
|
3525
|
+
|
3526
|
+
mergeLength++;
|
3527
|
+
mergePtr++;
|
3528
|
+
}
|
3529
|
+
|
3530
|
+
while (sourceIndex < sourceLength) {
|
3531
|
+
mergePtr->readID = sourcePtr->readID;
|
3532
|
+
setShortReadMarkerPosition(mergePtr, sourcePtr->position);
|
3533
|
+
setShortReadMarkerOffset(mergePtr, sourcePtr->offset);
|
3534
|
+
mergeLength++;
|
3535
|
+
mergePtr++;
|
3536
|
+
sourceIndex++;
|
3537
|
+
sourcePtr++;
|
3538
|
+
}
|
3539
|
+
|
3540
|
+
while (targetIndex < targetLength) {
|
3541
|
+
mergePtr->readID = targetPtr->readID;
|
3542
|
+
setShortReadMarkerPosition(mergePtr, targetPtr->position);
|
3543
|
+
setShortReadMarkerOffset(mergePtr, targetPtr->offset);
|
3544
|
+
mergeLength++;
|
3545
|
+
mergePtr++;
|
3546
|
+
targetIndex++;
|
3547
|
+
targetPtr++;
|
3548
|
+
}
|
3549
|
+
|
3550
|
+
free(targetArray);
|
3551
|
+
graph->nodeReads[targetID] = mergeArray;
|
3552
|
+
graph->nodeReadCounts[targetID] = mergeLength;
|
3553
|
+
|
3554
|
+
free(sourceArray);
|
3555
|
+
}
|
3556
|
+
|
3557
|
+
void mergeNodeReads(Node * target, Node * source, Graph * graph)
|
3558
|
+
{
|
3559
|
+
IDnum sourceID, sourceLength;
|
3560
|
+
ShortReadMarker *sourceArray;
|
3561
|
+
|
3562
|
+
if (graph->nodeReads == NULL || source == NULL || target == NULL)
|
3563
|
+
return;
|
3564
|
+
|
3565
|
+
sourceID = getNodeID(source) + graph->nodeCount;
|
3566
|
+
sourceArray = graph->nodeReads[sourceID];
|
3567
|
+
sourceLength = graph->nodeReadCounts[sourceID];
|
3568
|
+
|
3569
|
+
if (sourceArray == NULL)
|
3570
|
+
return;
|
3571
|
+
|
3572
|
+
graph->nodeReads[sourceID] = NULL;
|
3573
|
+
graph->nodeReadCounts[sourceID] = 0;
|
3574
|
+
|
3575
|
+
injectShortReads(sourceArray, sourceLength, target, graph);
|
3576
|
+
}
|
3577
|
+
|
3578
|
+
void foldSymmetricalNodeReads(Node * node, Graph * graph)
|
3579
|
+
{
|
3580
|
+
IDnum targetID, targetLength, targetIndex;
|
3581
|
+
IDnum sourceID, sourceLength, sourceIndex;
|
3582
|
+
IDnum targetVal = 0;
|
3583
|
+
IDnum sourceVal = 0;
|
3584
|
+
IDnum mergeLength;
|
3585
|
+
ShortReadMarker *sourceArray, *targetArray, *mergeArray,
|
3586
|
+
*mergeArray2;
|
3587
|
+
ShortReadMarker *sourcePtr, *targetPtr, *mergePtr, *mergePtr2;
|
3588
|
+
|
3589
|
+
if (graph->nodeReads == NULL || node == NULL)
|
3590
|
+
return;
|
3591
|
+
|
3592
|
+
sourceID = getNodeID(node) + graph->nodeCount;
|
3593
|
+
sourceArray = graph->nodeReads[sourceID];
|
3594
|
+
sourceLength = graph->nodeReadCounts[sourceID];
|
3595
|
+
sourcePtr = sourceArray;
|
3596
|
+
|
3597
|
+
targetID = -getNodeID(node) + graph->nodeCount;
|
3598
|
+
targetArray = graph->nodeReads[targetID];
|
3599
|
+
targetLength = graph->nodeReadCounts[targetID];
|
3600
|
+
targetPtr = targetArray;
|
3601
|
+
|
3602
|
+
if (sourceArray == NULL && targetArray == NULL)
|
3603
|
+
return;
|
3604
|
+
|
3605
|
+
mergeArray =
|
3606
|
+
mallocOrExit(sourceLength +
|
3607
|
+
targetLength, ShortReadMarker);
|
3608
|
+
mergeArray2 =
|
3609
|
+
mallocOrExit(sourceLength +
|
3610
|
+
targetLength, ShortReadMarker);
|
3611
|
+
mergePtr = mergeArray;
|
3612
|
+
mergePtr2 = mergeArray2;
|
3613
|
+
|
3614
|
+
mergeLength = 0;
|
3615
|
+
sourceIndex = 0;
|
3616
|
+
targetIndex = 0;
|
3617
|
+
if (targetIndex < targetLength)
|
3618
|
+
targetVal = targetPtr->readID;
|
3619
|
+
if (sourceIndex < sourceLength)
|
3620
|
+
sourceVal = sourcePtr->readID;
|
3621
|
+
|
3622
|
+
while (sourceIndex < sourceLength && targetIndex < targetLength) {
|
3623
|
+
if (sourceVal < targetVal) {
|
3624
|
+
mergePtr->readID = sourceVal;
|
3625
|
+
setShortReadMarkerPosition(mergePtr, -1);
|
3626
|
+
setShortReadMarkerOffset(mergePtr, -1);
|
3627
|
+
mergePtr2->readID = sourceVal;
|
3628
|
+
setShortReadMarkerPosition(mergePtr2, -1);
|
3629
|
+
setShortReadMarkerOffset(mergePtr2, -1);
|
3630
|
+
sourceIndex++;
|
3631
|
+
sourcePtr++;
|
3632
|
+
if (sourceIndex < sourceLength)
|
3633
|
+
sourceVal = sourcePtr->readID;
|
3634
|
+
} else if (sourceVal == targetVal) {
|
3635
|
+
mergePtr->readID = sourceVal;
|
3636
|
+
setShortReadMarkerPosition(mergePtr, -1);
|
3637
|
+
setShortReadMarkerOffset(mergePtr, -1);
|
3638
|
+
mergePtr2->readID = sourceVal;
|
3639
|
+
setShortReadMarkerPosition(mergePtr2, -1);
|
3640
|
+
setShortReadMarkerOffset(mergePtr2, -1);
|
3641
|
+
sourceIndex++;
|
3642
|
+
sourcePtr++;
|
3643
|
+
if (sourceIndex < sourceLength)
|
3644
|
+
sourceVal = sourcePtr->readID;
|
3645
|
+
targetIndex++;
|
3646
|
+
targetPtr++;
|
3647
|
+
if (targetIndex < targetLength)
|
3648
|
+
targetVal = targetPtr->readID;
|
3649
|
+
} else {
|
3650
|
+
mergePtr->readID = targetVal;
|
3651
|
+
setShortReadMarkerPosition(mergePtr, -1);
|
3652
|
+
setShortReadMarkerOffset(mergePtr, -1);
|
3653
|
+
mergePtr2->readID = targetVal;
|
3654
|
+
setShortReadMarkerPosition(mergePtr2, -1);
|
3655
|
+
setShortReadMarkerOffset(mergePtr2, -1);
|
3656
|
+
targetIndex++;
|
3657
|
+
targetPtr++;
|
3658
|
+
if (targetIndex < targetLength)
|
3659
|
+
targetVal = targetPtr->readID;
|
3660
|
+
}
|
3661
|
+
|
3662
|
+
mergeLength++;
|
3663
|
+
mergePtr++;
|
3664
|
+
mergePtr2++;
|
3665
|
+
}
|
3666
|
+
|
3667
|
+
while (sourceIndex < sourceLength) {
|
3668
|
+
mergePtr->readID = sourcePtr->readID;
|
3669
|
+
setShortReadMarkerPosition(mergePtr, -1);
|
3670
|
+
setShortReadMarkerOffset(mergePtr, -1);
|
3671
|
+
mergePtr2->readID = sourcePtr->readID;
|
3672
|
+
setShortReadMarkerPosition(mergePtr2, -1);
|
3673
|
+
setShortReadMarkerOffset(mergePtr2, -1);
|
3674
|
+
mergeLength++;
|
3675
|
+
mergePtr++;
|
3676
|
+
mergePtr2++;
|
3677
|
+
sourceIndex++;
|
3678
|
+
sourcePtr++;
|
3679
|
+
}
|
3680
|
+
|
3681
|
+
while (targetIndex < targetLength) {
|
3682
|
+
mergePtr->readID = targetPtr->readID;
|
3683
|
+
setShortReadMarkerPosition(mergePtr, -1);
|
3684
|
+
setShortReadMarkerOffset(mergePtr, -1);
|
3685
|
+
mergePtr2->readID = targetPtr->readID;
|
3686
|
+
setShortReadMarkerPosition(mergePtr2, -1);
|
3687
|
+
setShortReadMarkerOffset(mergePtr2, -1);
|
3688
|
+
mergeLength++;
|
3689
|
+
mergePtr++;
|
3690
|
+
mergePtr2++;
|
3691
|
+
targetIndex++;
|
3692
|
+
targetPtr++;
|
3693
|
+
}
|
3694
|
+
|
3695
|
+
free(targetArray);
|
3696
|
+
graph->nodeReads[targetID] = mergeArray;
|
3697
|
+
graph->nodeReadCounts[targetID] = mergeLength;
|
3698
|
+
|
3699
|
+
free(sourceArray);
|
3700
|
+
graph->nodeReads[sourceID] = mergeArray2;
|
3701
|
+
graph->nodeReadCounts[sourceID] = mergeLength;
|
3702
|
+
}
|
3703
|
+
|
3704
|
+
void shareReadStarts(Node * target, Node * source, Graph * graph)
|
3705
|
+
{
|
3706
|
+
ShortReadMarker *sourceArray;
|
3707
|
+
IDnum sourceLength, sourceID;
|
3708
|
+
|
3709
|
+
if (graph->nodeReads == NULL)
|
3710
|
+
return;
|
3711
|
+
|
3712
|
+
if (target == NULL || source == NULL)
|
3713
|
+
return;
|
3714
|
+
|
3715
|
+
sourceID = source->ID + graph->nodeCount;
|
3716
|
+
sourceArray = graph->nodeReads[sourceID];
|
3717
|
+
sourceLength = graph->nodeReadCounts[sourceID];
|
3718
|
+
|
3719
|
+
if (sourceArray == NULL)
|
3720
|
+
return;
|
3721
|
+
|
3722
|
+
spreadReadIDs(sourceArray, sourceLength, target, graph);
|
3723
|
+
}
|
3724
|
+
|
3725
|
+
ShortReadMarker **getNodeToReadMappings(Graph * graph)
|
3726
|
+
{
|
3727
|
+
return graph->nodeReads;
|
3728
|
+
}
|
3729
|
+
|
3730
|
+
IDnum getShortReadMarkerID(ShortReadMarker * marker)
|
3731
|
+
{
|
3732
|
+
return marker->readID;
|
3733
|
+
}
|
3734
|
+
|
3735
|
+
inline ShortLength getShortReadMarkerOffset(ShortReadMarker * marker)
|
3736
|
+
{
|
3737
|
+
return marker->offset;
|
3738
|
+
}
|
3739
|
+
|
3740
|
+
inline void setShortReadMarkerOffset(ShortReadMarker * marker,
|
3741
|
+
ShortLength offset)
|
3742
|
+
{
|
3743
|
+
marker->offset = offset;
|
3744
|
+
}
|
3745
|
+
|
3746
|
+
IDnum *getNodeReadCounts(Graph * graph)
|
3747
|
+
{
|
3748
|
+
return graph->nodeReadCounts;
|
3749
|
+
}
|
3750
|
+
|
3751
|
+
int getWordLength(Graph * graph)
|
3752
|
+
{
|
3753
|
+
return graph->wordLength;
|
3754
|
+
}
|
3755
|
+
|
3756
|
+
ShortReadMarker *getNodeReads(Node * node, Graph * graph)
|
3757
|
+
{
|
3758
|
+
IDnum id = node->ID + graph->nodeCount;
|
3759
|
+
return graph->nodeReads[id];
|
3760
|
+
}
|
3761
|
+
|
3762
|
+
IDnum getNodeReadCount(Node * node, Graph * graph)
|
3763
|
+
{
|
3764
|
+
IDnum id = node->ID + graph->nodeCount;
|
3765
|
+
return graph->nodeReadCounts[id];
|
3766
|
+
}
|
3767
|
+
|
3768
|
+
inline Coordinate getShortReadMarkerPosition(ShortReadMarker * marker)
|
3769
|
+
{
|
3770
|
+
return marker->position;
|
3771
|
+
}
|
3772
|
+
|
3773
|
+
inline void setShortReadMarkerPosition(ShortReadMarker * marker,
|
3774
|
+
Coordinate position)
|
3775
|
+
{
|
3776
|
+
if (position < -100)
|
3777
|
+
return;
|
3778
|
+
|
3779
|
+
marker->position = position;
|
3780
|
+
}
|
3781
|
+
|
3782
|
+
ShortReadMarker *getShortReadMarkerAtIndex(ShortReadMarker * array,
|
3783
|
+
IDnum index)
|
3784
|
+
{
|
3785
|
+
return &(array[index]);
|
3786
|
+
}
|
3787
|
+
|
3788
|
+
void destroyGraph(Graph * graph)
|
3789
|
+
{
|
3790
|
+
IDnum index;
|
3791
|
+
Node *node;
|
3792
|
+
for (index = 1; index <= graph->nodeCount; index++) {
|
3793
|
+
node = getNodeInGraph(graph, index);
|
3794
|
+
if (node != NULL)
|
3795
|
+
destroyNode(node, graph);
|
3796
|
+
}
|
3797
|
+
|
3798
|
+
if (graph->gapMarkers)
|
3799
|
+
deactivateGapMarkers(graph);
|
3800
|
+
|
3801
|
+
free(graph->nodes);
|
3802
|
+
destroyRecycleBin(nodeMemory);
|
3803
|
+
destroyRecycleBin(arcMemory);
|
3804
|
+
destroyAllPassageMarkers();
|
3805
|
+
free(graph->arcLookupTable);
|
3806
|
+
free(graph->nodeReads);
|
3807
|
+
free(graph->nodeReadCounts);
|
3808
|
+
free(graph);
|
3809
|
+
}
|
3810
|
+
|
3811
|
+
void setInsertLengths(Graph * graph, Category cat, Coordinate insertLength,
|
3812
|
+
Coordinate insertLength_std_dev)
|
3813
|
+
{
|
3814
|
+
graph->insertLengths[cat] = insertLength;
|
3815
|
+
graph->insertLengths_var[cat] =
|
3816
|
+
insertLength_std_dev * insertLength_std_dev;
|
3817
|
+
}
|
3818
|
+
|
3819
|
+
Coordinate getInsertLength(Graph * graph, Category cat)
|
3820
|
+
{
|
3821
|
+
return graph->insertLengths[cat / 2];
|
3822
|
+
}
|
3823
|
+
|
3824
|
+
double getInsertLength_var(Graph * graph, Category cat)
|
3825
|
+
{
|
3826
|
+
return graph->insertLengths_var[cat / 2];
|
3827
|
+
}
|
3828
|
+
|
3829
|
+
void activateGapMarkers(Graph * graph)
|
3830
|
+
{
|
3831
|
+
graph->gapMarkers =
|
3832
|
+
callocOrExit(graph->nodeCount + 1, GapMarker *);
|
3833
|
+
gapMarkerMemory = newRecycleBin(sizeof(GapMarker), GAPBLOCKSIZE);
|
3834
|
+
}
|
3835
|
+
|
3836
|
+
void deactivateGapMarkers(Graph * graph)
|
3837
|
+
{
|
3838
|
+
free(graph->gapMarkers);
|
3839
|
+
graph->gapMarkers = NULL;
|
3840
|
+
destroyRecycleBin(gapMarkerMemory);
|
3841
|
+
gapMarkerMemory = NULL;
|
3842
|
+
}
|
3843
|
+
|
3844
|
+
static GapMarker *allocateGapMarker()
|
3845
|
+
{
|
3846
|
+
return (GapMarker *) allocatePointer(gapMarkerMemory);
|
3847
|
+
}
|
3848
|
+
|
3849
|
+
void appendGap(Node * node, Coordinate length, Graph * graph)
|
3850
|
+
{
|
3851
|
+
IDnum nodeID = getNodeID(node);
|
3852
|
+
GapMarker *marker = allocateGapMarker();
|
3853
|
+
GapMarker *tmp;
|
3854
|
+
|
3855
|
+
marker->length = length;
|
3856
|
+
|
3857
|
+
if (nodeID > 0) {
|
3858
|
+
marker->position = node->length;
|
3859
|
+
marker->next = graph->gapMarkers[nodeID];
|
3860
|
+
graph->gapMarkers[nodeID] = marker;
|
3861
|
+
} else {
|
3862
|
+
for (tmp = graph->gapMarkers[-nodeID]; tmp != NULL;
|
3863
|
+
tmp = tmp->next)
|
3864
|
+
tmp->position += length;
|
3865
|
+
|
3866
|
+
marker->position = 0;
|
3867
|
+
marker->next = graph->gapMarkers[-nodeID];
|
3868
|
+
graph->gapMarkers[-nodeID] = marker;
|
3869
|
+
}
|
3870
|
+
|
3871
|
+
addBufferToDescriptor(node, length);
|
3872
|
+
}
|
3873
|
+
|
3874
|
+
void appendNodeGaps(Node * destination, Node * source, Graph * graph)
|
3875
|
+
{
|
3876
|
+
IDnum destinationID = getNodeID(destination);
|
3877
|
+
IDnum sourceID = getNodeID(source);
|
3878
|
+
GapMarker *marker;
|
3879
|
+
|
3880
|
+
if (graph->gapMarkers == NULL)
|
3881
|
+
return;
|
3882
|
+
|
3883
|
+
if (destinationID > 0 && sourceID > 0) {
|
3884
|
+
for (marker = graph->gapMarkers[sourceID]; marker != NULL;
|
3885
|
+
marker = marker->next)
|
3886
|
+
marker->position += destination->length;
|
3887
|
+
} else if (destinationID > 0 && sourceID < 0) {
|
3888
|
+
sourceID = -sourceID;
|
3889
|
+
for (marker = graph->gapMarkers[sourceID]; marker != NULL;
|
3890
|
+
marker = marker->next)
|
3891
|
+
marker->position =
|
3892
|
+
source->length + destination->length -
|
3893
|
+
marker->position - marker->length;
|
3894
|
+
} else if (destinationID < 0 && sourceID > 0) {
|
3895
|
+
destinationID = -destinationID;
|
3896
|
+
for (marker = graph->gapMarkers[destinationID];
|
3897
|
+
marker != NULL; marker = marker->next)
|
3898
|
+
marker->position += source->length;
|
3899
|
+
|
3900
|
+
for (marker = graph->gapMarkers[sourceID]; marker != NULL;
|
3901
|
+
marker = marker->next)
|
3902
|
+
marker->position =
|
3903
|
+
source->length - marker->position -
|
3904
|
+
marker->length;
|
3905
|
+
} else {
|
3906
|
+
destinationID = -destinationID;
|
3907
|
+
sourceID = -sourceID;
|
3908
|
+
for (marker = graph->gapMarkers[destinationID];
|
3909
|
+
marker != NULL; marker = marker->next)
|
3910
|
+
marker->position += source->length;
|
3911
|
+
}
|
3912
|
+
|
3913
|
+
if (graph->gapMarkers[destinationID] == NULL)
|
3914
|
+
graph->gapMarkers[destinationID] =
|
3915
|
+
graph->gapMarkers[sourceID];
|
3916
|
+
else {
|
3917
|
+
marker = graph->gapMarkers[destinationID];
|
3918
|
+
while (marker->next != NULL)
|
3919
|
+
marker = marker->next;
|
3920
|
+
marker->next = graph->gapMarkers[sourceID];
|
3921
|
+
}
|
3922
|
+
|
3923
|
+
graph->gapMarkers[sourceID] = NULL;
|
3924
|
+
}
|
3925
|
+
|
3926
|
+
GapMarker *getGap(Node * node, Graph * graph)
|
3927
|
+
{
|
3928
|
+
IDnum nodeID = getNodeID(node);
|
3929
|
+
|
3930
|
+
if (graph->gapMarkers == NULL)
|
3931
|
+
return NULL;
|
3932
|
+
|
3933
|
+
if (nodeID < 0)
|
3934
|
+
nodeID = -nodeID;
|
3935
|
+
|
3936
|
+
return graph->gapMarkers[nodeID];
|
3937
|
+
}
|
3938
|
+
|
3939
|
+
GapMarker *getNextGap(GapMarker * marker)
|
3940
|
+
{
|
3941
|
+
return marker->next;
|
3942
|
+
}
|
3943
|
+
|
3944
|
+
Coordinate getGapStart(GapMarker * marker)
|
3945
|
+
{
|
3946
|
+
return marker->position;
|
3947
|
+
}
|
3948
|
+
|
3949
|
+
Coordinate getGapFinish(GapMarker * marker)
|
3950
|
+
{
|
3951
|
+
return marker->position + marker->length;
|
3952
|
+
}
|
3953
|
+
|
3954
|
+
void reallocateNodeDescriptor(Node * node, Coordinate length) {
|
3955
|
+
Coordinate arrayLength, index, shift;
|
3956
|
+
Node * twin = node->twinNode;
|
3957
|
+
Descriptor * array;
|
3958
|
+
Nucleotide nucleotide;
|
3959
|
+
|
3960
|
+
if (length < node->length)
|
3961
|
+
exitErrorf(EXIT_FAILURE, true, "Sum of node lengths smaller than first!");
|
3962
|
+
|
3963
|
+
shift = length - node->length;
|
3964
|
+
|
3965
|
+
arrayLength = length / 4;
|
3966
|
+
if (length % 4)
|
3967
|
+
arrayLength++;
|
3968
|
+
|
3969
|
+
node->descriptor = reallocOrExit(node->descriptor, arrayLength, Descriptor);
|
3970
|
+
|
3971
|
+
array = callocOrExit(arrayLength, Descriptor);
|
3972
|
+
for (index = node->length - 1; index >= 0; index--) {
|
3973
|
+
nucleotide = getNucleotideInDescriptor(twin->descriptor, index);
|
3974
|
+
writeNucleotideInDescriptor(nucleotide, array, index + shift);
|
3975
|
+
}
|
3976
|
+
|
3977
|
+
free(twin->descriptor);
|
3978
|
+
twin->descriptor = array;
|
3979
|
+
}
|
3980
|
+
|
3981
|
+
boolean doubleStrandedGraph(Graph * graph) {
|
3982
|
+
return graph->double_stranded;
|
3983
|
+
}
|