bio-velvet_underground 0.0.1

data/ext/src/src/preGraph.h

@@ -0,0 +1,106 @@
+/*
+Copyright 2007, 2008 Daniel Zerbino (zerbino@ebi.ac.uk)
+
+    This file is part of Velvet.
+
+    Velvet is free software; you can redistribute it and/or modify
+    it under the terms of the GNU General Public License as published by
+    the Free Software Foundation; either version 2 of the License, or
+    (at your option) any later version.
+
+    Velvet is distributed in the hope that it will be useful,
+    but WITHOUT ANY WARRANTY; without even the implied warranty of
+    MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.  See the
+    GNU General Public License for more details.
+
+    You should have received a copy of the GNU General Public License
+    along with Velvet; if not, write to the Free Software
+    Foundation, Inc., 51 Franklin St, Fifth Floor, Boston, MA  02110-1301  USA
+
+*/
+#ifndef _PREGRAPH_H_
+#define _PREGRAPH_H_
+
+////////////////////////////////////////////////////////////
+// PreNode functions
+////////////////////////////////////////////////////////////
+
+void destroyPreNode_pg(IDnum preNode, PreGraph * preGraph);
+
+// Locator
+PreNode *getPreNodeInPreGraph_pg(PreGraph * preGraph, IDnum preNodeID);
+
+// PreArc info 
+PreArcI getPreArc_pg(IDnum preNodeID, PreGraph * preGraph);
+boolean hasSinglePreArc_pg(IDnum preNodeID, PreGraph * graph);
+char simplePreArcCount_pg(IDnum preNodeID, PreGraph * preGraph);
+
+// Descriptor
+Coordinate getPreNodeLength_pg(IDnum preNodeID, PreGraph * preGraph);
+void setPreNodeDescriptor_pg(Descriptor * descr, Coordinate length, IDnum preNodeID, PreGraph * preGraph);
+void appendDescriptors_pg(Descriptor ** start, int * writeOffset, IDnum preNodeID, PreGraph* preGraph, boolean initial);
+
+////////////////////////////////////////////////////////////
+// PreMarker functions
+////////////////////////////////////////////////////////////
+
+boolean referenceMarkersAreActivated_pg(PreGraph * preGraph);
+void allocatePreMarkerCountSpace_pg(PreGraph * preGraph);
+void incrementNodeReferenceMarkerCount_pg(PreGraph * preGraph, IDnum preNodeID);
+void allocatePreMarkerSpace_pg(PreGraph * preGraph);
+PreMarker * addPreMarker_pg(PreGraph * preGraph, IDnum nodeID, IDnum seqID, Coordinate * start, PreMarker * previous);
+void concatenateReferenceMarkers_pg(IDnum preNodeAID, IDnum preNodeBID, PreGraph * preGraph, Coordinate totalOffset);
+boolean hasPreMarkers(IDnum nodeID, PreGraph * preGraph);
+
+////////////////////////////////////////////////////////////
+// PreArc functions
+////////////////////////////////////////////////////////////
+
+// Creators/destructor
+PreArcI createPreArc_pg(IDnum originID, IDnum destinationID,
+			PreGraph * preGraph);
+void createAnalogousPreArc_pg(IDnum originID, IDnum destinationID,
+			      PreArcI refPreArc, PreGraph * preGraph);
+void destroyPreArc_pg(PreArcI preArc, PreGraph * preGraph);
+
+// Multiplicity
+void setMultiplicity_pg(PreArcI preArc, IDnum mult);
+IDnum getMultiplicity_pg(PreArcI preArc);
+
+// Extremities
+IDnum getDestination_pg(PreArcI preArc, IDnum nodeID);
+IDnum getOtherEnd_pg(PreArcI preArc, IDnum preNodeID);
+
+// Finding preArcs
+PreArcI getPreArcBetweenPreNodes_pg(IDnum originID, IDnum destinationID,
+				    PreGraph * preGraph);
+PreArcI getNextPreArc_pg(PreArcI preArc, IDnum originPreNodeID);
+
+// Misc
+boolean isLoop_pg(PreArcI preArc);
+
+////////////////////////////////////////////////////////////
+// PreGraph functions
+////////////////////////////////////////////////////////////
+
+// Memory allocation
+PreGraph *emptyPreGraph_pg(IDnum sequenceCount, IDnum referenceCount, int wordLength, boolean double_strand);
+void allocatePreNodeSpace_pg(PreGraph * preGraph, IDnum preNodeCount);
+void addPreNodeToPreGraph_pg(PreGraph * preGraph, Coordinate start,
+			     Coordinate stop, SequencesReader *seqReadInfo,
+			     Kmer * initialKmer, IDnum ID);
+
+// Deallocation
+void destroyPreGraph_pg(PreGraph * preGraph);
+
+// Dimensions
+IDnum preNodeCount_pg(PreGraph * preGraph);
+IDnum sequenceCount_pg(PreGraph * preGraph);
+void renumberPreNodes_pg(PreGraph * preGraph);
+
+// File IO
+void exportPreGraph_pg(char *filename, PreGraph * preGraph);
+
+int getWordLength_pg(PreGraph * preGraph);
+
+#endif

data/ext/src/src/preGraphConstruction.c

@@ -0,0 +1,990 @@
+/*
+Copyright 2007, 2008 Daniel Zerbino (zerbino@ebi.ac.uk)
+
+    This file is part of Velvet.
+
+    Velvet is free software; you can redistribute it and/or modify
+    it under the terms of the GNU General Public License as published by
+    the Free Software Foundation; either version 2 of the License, or
+    (at your option) any later version.
+
+    Velvet is distributed in the hope that it will be useful,
+    but WITHOUT ANY WARRANTY; without even the implied warranty of
+    MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.  See the
+    GNU General Public License for more details.
+
+    You should have received a copy of the GNU General Public License
+    along with Velvet; if not, write to the Free Software
+    Foundation, Inc., 51 Franklin St, Fifth Floor, Boston, MA  02110-1301  USA
+
+*/
+#include <stdlib.h>
+#include <stdio.h>
+#include <string.h>
+#include <ctype.h>
+
+#ifdef _OPENMP
+#include <omp.h>
+#endif
+
+#include "globals.h"
+#include "preGraph.h"
+#include "recycleBin.h"
+#include "roadMap.h"
+#include "readSet.h"
+#include "concatenatedPreGraph.h"
+#include "utility.h"
+#include "kmer.h"
+#include "tightString.h"
+#include "binarySequences.h"
+#define ADENINE 0
+#define CYTOSINE 1
+#define GUANINE 2
+#define THYMINE 3
+
+#ifdef _OPENMP
+
+Coordinate *annotationOffset = NULL;
+
+static omp_lock_t *nodeLocks = NULL;
+
+static void createNodeLocks(PreGraph *preGraph)
+{
+	IDnum nbNodes;
+	IDnum nodeIndex;
+
+	nbNodes = preNodeCount_pg(preGraph) + 1;
+	if (nodeLocks)
+		free (nodeLocks);
+	nodeLocks = mallocOrExit(nbNodes, omp_lock_t);
+
+	#pragma omp parallel for
+	for (nodeIndex = 0; nodeIndex < nbNodes; nodeIndex++)
+		omp_init_lock(nodeLocks + nodeIndex);
+}
+
+static void lockNode(IDnum preNodeID)
+{
+	omp_set_lock(nodeLocks + preNodeID);
+}
+
+static void unLockNode(IDnum preNodeID)
+{
+	omp_unset_lock(nodeLocks + preNodeID);
+}
+
+static void lockTwoNodes(IDnum preNodeID, IDnum preNode2ID)
+{
+	if (preNodeID < 0)
+		preNodeID = -preNodeID;
+	if (preNode2ID < 0)
+		preNode2ID = -preNode2ID;
+
+	/* Lock lowest ID first to avoid deadlocks */
+	if (preNodeID == preNode2ID)
+		omp_set_lock (nodeLocks + preNodeID);
+	else if (preNodeID < preNode2ID)
+	{
+		omp_set_lock (nodeLocks + preNodeID);
+		omp_set_lock (nodeLocks + preNode2ID);
+	}
+	else
+	{
+		omp_set_lock (nodeLocks + preNode2ID);
+		omp_set_lock (nodeLocks + preNodeID);
+	}
+}
+
+static void unLockTwoNodes(IDnum preNodeID, IDnum preNode2ID)
+{
+	if (preNodeID < 0)
+		preNodeID = -preNodeID;
+	if (preNode2ID < 0)
+		preNode2ID = -preNode2ID;
+
+	omp_unset_lock (nodeLocks + preNodeID);
+	if (preNodeID != preNode2ID)
+		omp_unset_lock (nodeLocks + preNode2ID);
+}
+#endif
+
+// Internal structure used to mark the ends of an Annotation
+struct insertionMarker_st {
+	Annotation *annot;
+	boolean isStart;
+}  ATTRIBUTE_PACKED;
+
+Coordinate getInsertionMarkerPosition(InsertionMarker * marker)
+{
+	if (marker->isStart)
+		return getStart(marker->annot);
+	else
+		return getFinish(marker->annot);
+}
+
+int compareInsertionMarkers(const void *A, const void *B)
+{
+	Coordinate Apos =
+	    getInsertionMarkerPosition((InsertionMarker *) A);
+	Coordinate Bpos =
+	    getInsertionMarkerPosition((InsertionMarker *) B);
+
+	if (Apos < Bpos)
+		return -1;
+	else if (Apos == Bpos)
+		return 0;
+	else
+		return 1;
+}
+
+// Applies mergeSort to each insertion marker list (in order of position)
+static void
+orderInsertionMarkers(InsertionMarker ** insMarkers,
+		      IDnum * markerCounters, RoadMapArray * rdmaps)
+{
+	IDnum sequenceIndex;
+	IDnum sequenceCounter = rdmaps->length;
+
+	velvetLog("Ordering insertion markers\n");
+#ifdef _OPENMP
+	#pragma omp parallel for
+#endif
+	for (sequenceIndex = 1; sequenceIndex <= sequenceCounter;
+	     sequenceIndex++) {
+		qsort(insMarkers[sequenceIndex],
+		      markerCounters[sequenceIndex],
+		      sizeof(InsertionMarker), compareInsertionMarkers);
+	}
+}
+
+// Creates insertion marker lists 
+static void
+setInsertionMarkers(RoadMapArray * rdmaps,
+		    IDnum * markerCounters,
+		    InsertionMarker ** veryLastMarker,
+		    InsertionMarker ** insertionMarkers)
+{
+	IDnum sequenceCounter = rdmaps->length;
+	IDnum sequenceIndex, sequenceIndex2;
+	Coordinate totalCount = 0;
+	RoadMap *rdmap;
+	Annotation *annot = rdmaps->annotations;
+	InsertionMarker *nextMarker, *newMarker;
+	IDnum annotIndex, lastAnnotIndex;
+	InsertionMarker **insMarkers =
+	    callocOrExit(rdmaps->length + 1, InsertionMarker *);
+	// Counting insertion markers
+	for (sequenceIndex = 1; sequenceIndex < sequenceCounter + 1;
+	     sequenceIndex++) {
+		//velvetLog("Going through sequence %d\n", sequenceIndex);
+		rdmap = getRoadMapInArray(rdmaps, sequenceIndex - 1);
+		lastAnnotIndex = getAnnotationCount(rdmap);
+
+		// Set insertion markers in previous sequences :
+
+		for (annotIndex = 0; annotIndex < lastAnnotIndex;
+		     annotIndex++) {
+			if (getAnnotSequenceID(annot) > 0) {
+				markerCounters[getAnnotSequenceID(annot)]
+				    += 2;
+			} else {
+				markerCounters[-getAnnotSequenceID(annot)]
+				    += 2;
+			}
+			totalCount += 2;
+			annot = getNextAnnotation(annot);
+		}
+	}
+
+	// Allocating space
+	*insertionMarkers = callocOrExit(totalCount, InsertionMarker);
+	*veryLastMarker = *insertionMarkers + totalCount;
+
+	// Pointing each node to its space      
+	nextMarker = *insertionMarkers;
+	for (sequenceIndex = 1; sequenceIndex < sequenceCounter + 1;
+	     sequenceIndex++) {
+		insMarkers[sequenceIndex] = nextMarker;
+		nextMarker = nextMarker + markerCounters[sequenceIndex];
+		markerCounters[sequenceIndex] = 0;
+	}
+
+	// Filling up space with data
+	annot = rdmaps->annotations;
+	for (sequenceIndex = 1; sequenceIndex < sequenceCounter + 1;
+	     sequenceIndex++) {
+		//velvetLog("Going through sequence %d\n", sequenceIndex);
+		rdmap = getRoadMapInArray(rdmaps, sequenceIndex - 1);
+		lastAnnotIndex = getAnnotationCount(rdmap);
+
+		// Set insertion markers in previous sequences :
+
+		for (annotIndex = 0; annotIndex < lastAnnotIndex;
+		     annotIndex++) {
+			sequenceIndex2 = getAnnotSequenceID(annot);
+			if (sequenceIndex2 > 0) {
+				newMarker =
+				    insMarkers[sequenceIndex2] +
+				    (markerCounters[sequenceIndex2])++;
+				newMarker->annot = annot;
+				newMarker->isStart = true;
+
+				newMarker =
+				    insMarkers[sequenceIndex2] +
+				    (markerCounters[sequenceIndex2])++;
+				newMarker->annot = annot;
+				newMarker->isStart = false;
+			} else {
+				incrementAnnotationCoordinates(annot);
+
+				newMarker =
+				    insMarkers[-sequenceIndex2] +
+				    (markerCounters[-sequenceIndex2])++;
+				newMarker->annot = annot;
+				newMarker->isStart = true;
+
+				newMarker =
+				    insMarkers[-sequenceIndex2] +
+				    (markerCounters[-sequenceIndex2])++;
+				newMarker->annot = annot;
+				newMarker->isStart = false;
+			}
+			annot = getNextAnnotation(annot);
+		}
+	}
+
+	orderInsertionMarkers(insMarkers, markerCounters, rdmaps);
+	free(insMarkers);
+}
+
+// Counts how many preNodes are to be created to allocate appropriate memory
+static void
+countPreNodes(RoadMapArray * rdmaps, PreGraph * preGraph,
+	      IDnum * markerCounters, InsertionMarker * insertionMarkers,
+	      InsertionMarker * veryLastMarker)
+{
+	Annotation *annot = rdmaps->annotations;
+	InsertionMarker *currentMarker = insertionMarkers;
+	IDnum markerIndex, lastMarkerIndex;
+	IDnum sequenceIndex;
+	Coordinate currentPosition, nextStop;
+	IDnum preNodeCounter = 0;
+	RoadMap *rdmap;
+	IDnum annotIndex, lastAnnotIndex;
+
+	// Now that we have read all of the annotations, we go on to create the preNodes and tie them up
+	for (sequenceIndex = 1;
+	     sequenceIndex <= sequenceCount_pg(preGraph);
+	     sequenceIndex++) {
+		rdmap = getRoadMapInArray(rdmaps, sequenceIndex - 1);
+		annotIndex = 0;
+		lastAnnotIndex = getAnnotationCount(rdmap);
+		markerIndex = 0;
+		lastMarkerIndex = markerCounters[sequenceIndex];
+		currentPosition = 0;
+
+
+		while (annotIndex < lastAnnotIndex) {
+			if (markerIndex == lastMarkerIndex
+			    || getPosition(annot) <=
+			    getInsertionMarkerPosition(currentMarker))
+				nextStop = getPosition(annot);
+			else
+				nextStop =
+				    getInsertionMarkerPosition
+				    (currentMarker);
+
+			if (currentPosition != nextStop) {
+				preNodeCounter++;
+				currentPosition = nextStop;
+			}
+
+			while (markerIndex < lastMarkerIndex
+			       && getInsertionMarkerPosition(currentMarker)
+			       == currentPosition) {
+				currentMarker++;
+				markerIndex++;
+			}
+
+			while (annotIndex < lastAnnotIndex
+			       && getPosition(annot) == currentPosition) {
+				annot = getNextAnnotation(annot);
+				annotIndex++;
+			}
+
+		}
+
+		while (markerIndex < lastMarkerIndex) {
+			if (currentPosition ==
+			    getInsertionMarkerPosition(currentMarker)) {
+				currentMarker++;
+				markerIndex++;
+			} else {
+				preNodeCounter++;
+				currentPosition =
+				    getInsertionMarkerPosition
+				    (currentMarker);
+			}
+		}
+	}
+
+	allocatePreNodeSpace_pg(preGraph, preNodeCounter);
+}
+
+static void convertInsertionMarkers(InsertionMarker * insertionMarkers,
+				    InsertionMarker * veryLastMarker,
+				    IDnum * chains)
+{
+	InsertionMarker *marker;
+	Annotation *annot;
+
+	for (marker = insertionMarkers; marker != veryLastMarker; marker++) {
+		annot = marker->annot;
+
+		if (getAnnotSequenceID(annot) > 0) {
+			if (marker->isStart) {
+				if (getStartID(annot) == 0)
+					setStartID(annot,
+						   chains
+						   [getAnnotSequenceID
+						    (annot)]);
+				else
+					setStartID(annot,
+						   getStartID(annot) + 1);
+			}
+		} else {
+			if (marker->isStart)
+				setStartID(annot, -getStartID(annot));
+			else {
+				if (getFinishID(annot) == 0)
+					setFinishID(annot,
+						    -chains
+						    [-getAnnotSequenceID
+						     (annot)]);
+				else
+					setFinishID(annot,
+						    -getFinishID(annot) -
+						    1);
+			}
+		}
+	}
+
+	free(insertionMarkers);
+}
+
+static void convertMarker(InsertionMarker * marker, IDnum nodeID)
+{
+	if (marker->isStart)
+		setStartID(marker->annot, nodeID);
+	else
+		setFinishID(marker->annot, nodeID);
+}
+
+// Creates the preNode using insertion marker and annotation lists for each sequence
+static void
+// Creates the preNode using insertion marker and annotation lists for each sequence
+createPreNodes(RoadMapArray * rdmaps, PreGraph * preGraph,
+	       IDnum * markerCounters, InsertionMarker * insertionMarkers,
+	       InsertionMarker * veryLastMarker, IDnum * chains,
+	       SequencesReader *seqReadInfo, int WORDLENGTH)
+{
+	char *sequenceFilename = seqReadInfo->m_seqFilename;
+	Annotation *annot = rdmaps->annotations;
+	IDnum latestPreNodeID;
+	InsertionMarker *currentMarker = insertionMarkers;
+	IDnum sequenceIndex;
+	Coordinate currentPosition, nextStop;
+	IDnum preNodeCounter = 1;
+	FILE *file = NULL;
+	char line[50000];
+	int lineLength = 50000;
+	Coordinate readIndex;
+	boolean tooShort;
+	Kmer initialKmer;
+	char c;
+	RoadMap *rdmap;
+	IDnum annotIndex, lastAnnotIndex;
+	IDnum markerIndex, lastMarkerIndex;
+
+	if (!seqReadInfo->m_bIsBinary) {
+		file = fopen(sequenceFilename, "r");
+	if (file == NULL) 
+		exitErrorf(EXIT_FAILURE, true, "Could not read %s", sequenceFilename);
+	// Reading sequence descriptor in first line
+	if (sequenceCount_pg(preGraph) > 0 && !fgets(line, lineLength, file))
+		exitErrorf(EXIT_FAILURE, true, "%s incomplete.", sequenceFilename);
+		seqReadInfo->m_pFile = file;
+	}
+
+	// Now that we have read all of the annotations, we go on to create the preNodes and tie them up
+	for (sequenceIndex = 1;
+	     sequenceIndex <= sequenceCount_pg(preGraph);
+	     sequenceIndex++) {
+		if (sequenceIndex % 1000000 == 0)
+			velvetLog("Sequence %li / %li\n", (long) sequenceIndex,
+			       (long) sequenceCount_pg(preGraph));
+
+		if (!seqReadInfo->m_bIsBinary) {
+		while (line[0] != '>')
+			if (!fgets(line, lineLength, file))
+				exitErrorf(EXIT_FAILURE, true, "%s incomplete.", sequenceFilename);
+		}
+
+		rdmap = getRoadMapInArray(rdmaps, sequenceIndex - 1);
+		annotIndex = 0;
+		lastAnnotIndex = getAnnotationCount(rdmap);
+		markerIndex = 0;
+		lastMarkerIndex = markerCounters[sequenceIndex];
+		currentPosition = 0;
+
+		// Reading first (k-1) nucleotides
+		tooShort = false;
+		clearKmer(&initialKmer);
+		//velvetLog("Initial kmer: ");
+		TightString *tString = NULL;
+		char *strString = NULL;
+		if (seqReadInfo->m_bIsBinary) {
+			tString = getTightStringInArray(seqReadInfo->m_sequences->tSequences, sequenceIndex - 1);
+			strString = readTightString(tString);
+		}
+		for (readIndex = 0; readIndex < WORDLENGTH - 1;
+		     readIndex++) {
+			if (seqReadInfo->m_bIsBinary) {
+				if (readIndex >= tString->length) {
+					tooShort = true;
+					break;
+				}
+
+				c = strString[readIndex];
+			} else {
+			c = getc(file);
+			while (c == '\n' || c == '\r') 
+				c = getc(file);
+	
+			if (c == '>' || c == 'M' || c == EOF) {
+				ungetc(c, file);
+				tooShort = true;
+				break;
+			}
+			}
+			switch (c) {
+			case 'A':
+			case 'N':
+				pushNucleotide(&initialKmer, ADENINE);
+				break;
+			case 'C':
+				pushNucleotide(&initialKmer, CYTOSINE);
+				break;
+			case 'G':
+				pushNucleotide(&initialKmer, GUANINE);
+				break;
+			case 'T':
+				pushNucleotide(&initialKmer, THYMINE);
+				break;
+			default:
+				velvetLog
+				    ("Irregular sequence file: are you sure your Sequence and Roadmap file come from the same source?\n");
+				fflush(stdout);
+				abort();
+			}
+		}
+
+		if (tooShort) {
+			//velvetLog("Skipping short read.. %d\n", sequenceIndex);
+			chains[sequenceIndex] = preNodeCounter;
+			if (seqReadInfo->m_bIsBinary) {
+				free(strString);
+			} else {
+			if (!fgets(line, lineLength, file) && sequenceIndex < sequenceCount_pg(preGraph))
+				exitErrorf(EXIT_FAILURE, true, "%s incomplete.", sequenceFilename);
+			}
+			continue;
+		}
+
+		char *currString = NULL;
+		if (seqReadInfo->m_bIsBinary) {
+			currString = &strString[readIndex];
+			seqReadInfo->m_ppCurrString = &currString;
+		}
+		latestPreNodeID = 0;
+
+		while (annotIndex < lastAnnotIndex) {
+			if (markerIndex == lastMarkerIndex
+			    || getPosition(annot) <=
+			    getInsertionMarkerPosition(currentMarker))
+				nextStop = getPosition(annot);
+			else {
+				nextStop =
+				    getInsertionMarkerPosition
+				    (currentMarker);
+			}
+
+			if (currentPosition != nextStop) {
+				if (seqReadInfo->m_bIsBinary) {
+					if (readIndex >= tString->length) {
+						velvetLog("readIndex %ld beyond string len %ld\n", (uint64_t) readIndex, (uint64_t) tString->length);
+						exit(1);
+					}
+				}
+				//if (sequenceIndex == 481)
+				//	velvetLog("Adding pre nodes from %lli to %lli\n", (long long) currentPosition, (long long) nextStop);
+				addPreNodeToPreGraph_pg(preGraph,
+							currentPosition,
+							nextStop,
+							seqReadInfo,
+							&initialKmer,
+							preNodeCounter);
+				if (latestPreNodeID == 0) {
+					chains[sequenceIndex] =
+					    preNodeCounter;
+				}
+				latestPreNodeID = preNodeCounter++;
+				currentPosition = nextStop;
+			}
+
+			while (markerIndex < lastMarkerIndex
+			       && getInsertionMarkerPosition(currentMarker)
+			       == nextStop) {
+				convertMarker(currentMarker,
+					      latestPreNodeID);
+				currentMarker++;
+				markerIndex++;
+			}
+
+			while (annotIndex < lastAnnotIndex
+			       && getPosition(annot) == nextStop) {
+				for (readIndex = 0;
+				     readIndex <
+				     getAnnotationLength(annot);
+				     readIndex++) {
+					if (seqReadInfo->m_bIsBinary) {
+						c = *currString;
+						currString += 1;   // increment the pointer
+					} else {
+					c = getc(file);
+					while (!isalpha(c))
+						c = getc(file);
+					}
+
+					//if (sequenceIndex == 481)
+					//	velvetLog("(%c)", c);
+					switch (c) {
+					case 'A':
+					case 'N':
+						pushNucleotide(&initialKmer, ADENINE);
+						break;
+					case 'C':
+						pushNucleotide(&initialKmer, CYTOSINE);
+						break;
+					case 'G':
+						pushNucleotide(&initialKmer, GUANINE);
+						break;
+					case 'T':
+						pushNucleotide(&initialKmer, THYMINE);
+						break;
+					default:
+						velvetLog
+						    ("Irregular sequence file: are you sure your Sequence and Roadmap file come from the same source?\n");
+						fflush(stdout);
+#ifdef DEBUG 
+						abort();
+#endif 
+						exit(1);
+					}
+				}
+
+				annot = getNextAnnotation(annot);
+				annotIndex++;
+			}
+
+		}
+
+		while (markerIndex < lastMarkerIndex) {
+			if (currentPosition ==
+			    getInsertionMarkerPosition(currentMarker)) {
+				convertMarker(currentMarker,
+					      latestPreNodeID);
+				currentMarker++;
+				markerIndex++;
+			} else {
+				nextStop =
+				    getInsertionMarkerPosition
+				    (currentMarker);
+				//if (sequenceIndex == 481)
+				//	velvetLog("Adding pre nodes from %lli to %lli\n", (long long) currentPosition, (long long) nextStop);
+				addPreNodeToPreGraph_pg(preGraph,
+							currentPosition,
+							nextStop, seqReadInfo,
+							&initialKmer,
+							preNodeCounter);
+				if (latestPreNodeID == 0)
+					chains[sequenceIndex] =
+					    preNodeCounter;
+				latestPreNodeID = preNodeCounter++;
+				currentPosition =
+				    getInsertionMarkerPosition
+				    (currentMarker);
+			}
+		}
+		if (seqReadInfo->m_bIsBinary) {
+			free(strString);
+		} else {
+		// End of sequence
+		if (!fgets(line, lineLength, file) && sequenceIndex < sequenceCount_pg(preGraph))
+			exitErrorf(EXIT_FAILURE, true, "%s incomplete.", sequenceFilename);
+		//velvetLog(" \n");
+		}
+
+		if (latestPreNodeID == 0)
+			chains[sequenceIndex] = preNodeCounter;
+	}
+
+	free(markerCounters);
+	if (!seqReadInfo->m_bIsBinary) {
+	fclose(file);
+	}
+
+}
+
+static void connectPreNodeToTheNext(IDnum * currentPreNodeID,
+				    IDnum nextPreNodeID,
+				    Coordinate * currentPosition,
+				    IDnum sequenceIndex,
+				    boolean isReference,
+				    PreGraph * preGraph)
+{
+	if (nextPreNodeID == 0)
+		return;
+
+#ifdef _OPENMP
+	lockTwoNodes(*currentPreNodeID, nextPreNodeID);
+#endif
+
+	if (isReference)
+		incrementNodeReferenceMarkerCount_pg(preGraph, nextPreNodeID);
+
+	if (!isReference && *currentPreNodeID != 0)
+		createPreArc_pg(*currentPreNodeID, nextPreNodeID,
+				preGraph);
+
+#ifdef _OPENMP
+	unLockTwoNodes(*currentPreNodeID, nextPreNodeID);
+#endif
+
+	*currentPreNodeID = nextPreNodeID;
+
+	*currentPosition +=
+	    getPreNodeLength_pg(*currentPreNodeID, preGraph);
+
+}
+
+static IDnum chooseNextInternalPreNode(IDnum currentPreNodeID,
+				       IDnum sequenceIndex,
+				       PreGraph * preGraph, IDnum * chains)
+{
+	if (currentPreNodeID >= preNodeCount_pg(preGraph))
+		return 0;
+	if (sequenceIndex >= sequenceCount_pg(preGraph))
+		return currentPreNodeID + 1;
+	if (currentPreNodeID + 1 < chains[sequenceIndex + 1])
+		return currentPreNodeID + 1;
+	return 0;
+}
+
+static void connectAnnotation(IDnum * currentPreNodeID, Annotation * annot,
+			      Coordinate * currentPosition,
+			      IDnum sequenceIndex, boolean isReference,
+			      PreGraph * preGraph)
+{
+	IDnum nextPreNodeID = getStartID(annot);
+
+	connectPreNodeToTheNext(currentPreNodeID, nextPreNodeID,
+				currentPosition, 
+				sequenceIndex, isReference, preGraph);
+
+	while (*currentPreNodeID != getFinishID(annot)) {
+		nextPreNodeID = (*currentPreNodeID) + 1;
+
+		connectPreNodeToTheNext(currentPreNodeID, nextPreNodeID,
+					currentPosition,
+					sequenceIndex,
+					isReference,
+					preGraph);
+	}
+}
+
+static void reConnectAnnotation(IDnum * currentPreNodeID, Annotation * annot,
+			      Coordinate * currentPosition,
+			      IDnum sequenceIndex, 
+			      PreGraph * preGraph,
+			      PreMarker ** previous)
+{
+	IDnum nextPreNodeID = getStartID(annot);
+
+#ifdef _OPENMP
+	lockNode(nextPreNodeID);
+#endif
+	*previous = addPreMarker_pg(preGraph, 
+			nextPreNodeID,
+			sequenceIndex,
+			currentPosition, 
+			*previous);
+#ifdef _OPENMP
+	unLockNode(nextPreNodeID);
+#endif
+
+	while (*currentPreNodeID != getFinishID(annot)) {
+		nextPreNodeID = (*currentPreNodeID) + 1;
+
+#ifdef _OPENMP
+		lockNode(nextPreNodeID);
+#endif
+		*previous = addPreMarker_pg(preGraph, 
+				nextPreNodeID,
+				sequenceIndex,
+				currentPosition,
+				*previous);
+#ifdef _OPENMP
+		unLockNode(nextPreNodeID);
+#endif
+		*currentPreNodeID = nextPreNodeID;
+	}
+}
+
+static void createPreMarkers(RoadMapArray * rdmaps, PreGraph * preGraph,
+			    IDnum * chains)
+{
+	IDnum sequenceIndex;
+	IDnum referenceCount = rdmaps->referenceCount;
+#ifndef _OPENMP
+	Annotation *annot = rdmaps->annotations;
+#endif
+
+#ifdef _OPENMP
+	int threads = omp_get_max_threads();
+	if (threads > 8)
+		threads = 8;
+
+	#pragma omp parallel for num_threads(threads)
+#endif
+	for (sequenceIndex = 1;
+	     sequenceIndex <= referenceCount;
+	     sequenceIndex++) {
+#ifdef _OPENMP
+		Annotation *annot = getAnnotationInArray(rdmaps->annotations, annotationOffset[sequenceIndex - 1]);
+#endif
+		RoadMap *rdmap;
+		Coordinate currentPosition, currentInternalPosition;
+		IDnum currentPreNodeID, nextInternalPreNodeID;
+		IDnum annotIndex, lastAnnotIndex;
+		PreMarker * previous;
+
+		if (sequenceIndex % 1000000 == 0)
+			velvetLog("Connecting %li / %li\n", (long) sequenceIndex,
+			       (long) sequenceCount_pg(preGraph));
+
+		rdmap = getRoadMapInArray(rdmaps, sequenceIndex - 1);
+		annotIndex = 0;
+		lastAnnotIndex = getAnnotationCount(rdmap);
+		nextInternalPreNodeID = chooseNextInternalPreNode
+		    (chains[sequenceIndex] - 1, sequenceIndex,
+		     preGraph, chains);
+
+		previous = NULL;
+		currentPosition = 0;
+		currentInternalPosition = 0;
+		currentPreNodeID = 0;
+		// Recursion up to last annotation
+		while (annotIndex < lastAnnotIndex
+		       || nextInternalPreNodeID != 0) {
+			if (annotIndex == lastAnnotIndex
+			    || (nextInternalPreNodeID != 0
+				&& currentInternalPosition <
+				getPosition(annot))) {
+#ifdef _OPENMP
+				lockNode(nextInternalPreNodeID);
+#endif
+				previous = addPreMarker_pg(preGraph, 
+						nextInternalPreNodeID,
+						sequenceIndex,
+						&currentPosition,
+						previous);
+#ifdef _OPENMP
+				unLockNode(nextInternalPreNodeID);
+#endif
+				currentPreNodeID = nextInternalPreNodeID;
+				nextInternalPreNodeID =
+				    chooseNextInternalPreNode
+				    (currentPreNodeID, sequenceIndex,
+				     preGraph, chains);
+				currentInternalPosition +=
+				    getPreNodeLength_pg(currentPreNodeID,
+							preGraph);
+
+			} else {
+				reConnectAnnotation(&currentPreNodeID, annot,
+						  &currentPosition,
+						  sequenceIndex, 
+						  preGraph,
+						  &previous);
+				annot = getNextAnnotation(annot);
+				annotIndex++;
+			}
+		}
+	}
+}
+
+// Threads each sequences and creates preArcs according to road map indications
+static void connectPreNodes(RoadMapArray * rdmaps, PreGraph * preGraph,
+			    IDnum * chains)
+{
+	IDnum sequenceIndex;
+	IDnum referenceCount = rdmaps->referenceCount;
+#ifdef _OPENMP
+	annotationOffset = mallocOrExit(rdmaps->length + 1, Coordinate);
+	annotationOffset[0] = 0;
+	for (sequenceIndex = 1; sequenceIndex <= rdmaps->length; sequenceIndex++)
+		annotationOffset[sequenceIndex] = annotationOffset[sequenceIndex - 1] +
+						  getAnnotationCount(getRoadMapInArray(rdmaps, sequenceIndex - 1));
+#else
+	Annotation *annot = rdmaps->annotations;
+#endif
+
+	if (rdmaps->referenceCount > 0) 
+		allocatePreMarkerCountSpace_pg(preGraph);
+
+#ifdef _OPENMP
+	int threads = omp_get_max_threads();
+	if (threads > 8)
+		threads = 8;
+
+	#pragma omp parallel for num_threads(threads)
+#endif
+	for (sequenceIndex = 1;
+	     sequenceIndex <= sequenceCount_pg(preGraph);
+	     sequenceIndex++) {
+#ifdef _OPENMP
+		Annotation *annot = getAnnotationInArray(rdmaps->annotations, annotationOffset[sequenceIndex - 1]);
+#endif
+		RoadMap *rdmap;
+		Coordinate currentPosition, currentInternalPosition;
+		IDnum currentPreNodeID, nextInternalPreNodeID;
+		IDnum annotIndex, lastAnnotIndex;
+		boolean isReference;
+
+		if (sequenceIndex % 1000000 == 0)
+			velvetLog("Connecting %li / %li\n", (long) sequenceIndex,
+			       (long) sequenceCount_pg(preGraph));
+
+		rdmap = getRoadMapInArray(rdmaps, sequenceIndex - 1);
+		annotIndex = 0;
+		lastAnnotIndex = getAnnotationCount(rdmap);
+		nextInternalPreNodeID = chooseNextInternalPreNode
+		    (chains[sequenceIndex] - 1, sequenceIndex,
+		     preGraph, chains);
+		isReference = (sequenceIndex <= referenceCount);
+
+		currentPosition = 0;
+		currentInternalPosition = 0;
+		currentPreNodeID = 0;
+		// Recursion up to last annotation
+		while (annotIndex < lastAnnotIndex
+		       || nextInternalPreNodeID != 0) {
+			if (annotIndex == lastAnnotIndex
+			    || (nextInternalPreNodeID != 0
+				&& currentInternalPosition <
+				getPosition(annot))) {
+				connectPreNodeToTheNext(&currentPreNodeID,
+							nextInternalPreNodeID,
+							&currentPosition,
+							sequenceIndex,
+							isReference,
+							preGraph);
+				nextInternalPreNodeID =
+				    chooseNextInternalPreNode
+				    (currentPreNodeID, sequenceIndex,
+				     preGraph, chains);
+				currentInternalPosition +=
+				    getPreNodeLength_pg(currentPreNodeID,
+							preGraph);
+
+			} else {
+				connectAnnotation(&currentPreNodeID, annot,
+						  &currentPosition,
+						  sequenceIndex, isReference,
+						  preGraph);
+				annot = getNextAnnotation(annot);
+				annotIndex++;
+			}
+		}
+	}
+
+	if (rdmaps->referenceCount > 0) {
+		allocatePreMarkerSpace_pg(preGraph);
+		createPreMarkers(rdmaps, preGraph, chains);	
+	}
+
+#ifdef _OPENMP
+	free(annotationOffset);
+	annotationOffset = NULL;
+#endif
+}
+
+// Post construction memory deallocation routine (of sorts, could certainly be optimized)
+static void
+cleanUpMemory(PreGraph * preGraph, RoadMapArray * rdmaps, IDnum * chains)
+{
+	// Killing off roadmaps
+	destroyRoadMapArray(rdmaps);
+
+	// Finishing off the chain markers
+	free(chains);
+}
+
+// The full monty, wrapped up in one function
+PreGraph *newPreGraph_pg(RoadMapArray * rdmapArray, SequencesReader *seqReadInfo)
+{
+	int WORDLENGTH = rdmapArray->WORDLENGTH;
+	IDnum sequenceCount = rdmapArray->length;
+	IDnum *markerCounters = callocOrExit(sequenceCount + 1, IDnum);
+	IDnum *chains = callocOrExit(sequenceCount + 1, IDnum);
+	InsertionMarker *insertionMarkers;
+	InsertionMarker *veryLastMarker;
+
+	PreGraph *preGraph =
+	    emptyPreGraph_pg(sequenceCount, rdmapArray->referenceCount, rdmapArray->WORDLENGTH, rdmapArray->double_strand);
+
+	velvetLog("Creating insertion markers\n");
+	setInsertionMarkers(rdmapArray, markerCounters, &veryLastMarker,
+			    &insertionMarkers);
+
+	velvetLog("Counting preNodes\n");
+	countPreNodes(rdmapArray, preGraph, markerCounters,
+		      insertionMarkers, veryLastMarker);
+
+	velvetLog("%li preNodes counted, creating them now\n",
+	       (long) preNodeCount_pg(preGraph));
+	createPreNodes(rdmapArray, preGraph, markerCounters,
+		       insertionMarkers, veryLastMarker, chains,
+		       seqReadInfo, WORDLENGTH);
+
+	velvetLog("Adjusting marker info...\n");
+	convertInsertionMarkers(insertionMarkers, veryLastMarker, chains);
+
+#ifdef _OPENMP
+	createNodeLocks(preGraph);
+#endif
+	velvetLog("Connecting preNodes\n");
+	connectPreNodes(rdmapArray, preGraph, chains);
+
+	velvetLog("Cleaning up memory\n");
+	cleanUpMemory(preGraph, rdmapArray, chains);
+#ifdef _OPENMP
+	free(nodeLocks);
+	nodeLocks = NULL;
+#endif 
+
+	velvetLog("Done creating preGraph\n");
+
+	return preGraph;
+}