bioroebe 0.10.80

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Files changed (802) hide show
  1. checksums.yaml +7 -0
  2. data/LICENSE.md +428 -0
  3. data/README.md +9280 -0
  4. data/bin/advanced_dotplot +7 -0
  5. data/bin/align_open_reading_frames +12 -0
  6. data/bin/all_positions_of_this_nucleotide +7 -0
  7. data/bin/aminoacid_composition +7 -0
  8. data/bin/aminoacid_frequencies +12 -0
  9. data/bin/aminoacid_substitution +7 -0
  10. data/bin/automatically_rename_this_fasta_file +7 -0
  11. data/bin/base_composition +7 -0
  12. data/bin/batch_create_windows_executables +7 -0
  13. data/bin/biomart_console +11 -0
  14. data/bin/bioroebe +27 -0
  15. data/bin/bioroebe_controller +10 -0
  16. data/bin/bioshell +26 -0
  17. data/bin/blosum_2D_table +12 -0
  18. data/bin/calculate_n50_value +12 -0
  19. data/bin/chunked_display +12 -0
  20. data/bin/codon_frequency +9 -0
  21. data/bin/codon_to_aminoacid +30 -0
  22. data/bin/colourize_this_fasta_sequence +14 -0
  23. data/bin/compact_fasta_file +7 -0
  24. data/bin/complement +7 -0
  25. data/bin/complementary_dna_strand +12 -0
  26. data/bin/complementary_rna_strand +12 -0
  27. data/bin/compseq +7 -0
  28. data/bin/consensus_sequence +17 -0
  29. data/bin/count_AT +12 -0
  30. data/bin/count_GC +12 -0
  31. data/bin/create_random_aminoacids +7 -0
  32. data/bin/decode_this_aminoacid_sequence +20 -0
  33. data/bin/deduce_aminoacid_sequence +13 -0
  34. data/bin/deduce_most_likely_aminoacid_sequence +7 -0
  35. data/bin/display_aminoacid_table +12 -0
  36. data/bin/display_open_reading_frames +7 -0
  37. data/bin/dna_to_aminoacid_sequence +7 -0
  38. data/bin/dna_to_rna +7 -0
  39. data/bin/downcase_chunked_display +12 -0
  40. data/bin/download_this_pdb +7 -0
  41. data/bin/fasta_index +7 -0
  42. data/bin/fetch_data_from_uniprot +12 -0
  43. data/bin/filter_away_invalid_nucleotides +12 -0
  44. data/bin/find_substring +19 -0
  45. data/bin/genbank_to_fasta +7 -0
  46. data/bin/hamming_distance +12 -0
  47. data/bin/input_as_dna +12 -0
  48. data/bin/is_palindrome +13 -0
  49. data/bin/leading_five_prime +7 -0
  50. data/bin/levensthein +7 -0
  51. data/bin/longest_ORF +14 -0
  52. data/bin/longest_substring +12 -0
  53. data/bin/n_stop_codons_in_this_sequence +15 -0
  54. data/bin/open_reading_frames +14 -0
  55. data/bin/overwrite_fasta_header +7 -0
  56. data/bin/palindrome_2D_structure +7 -0
  57. data/bin/palindrome_generator +7 -0
  58. data/bin/parse_fasta +7 -0
  59. data/bin/partner_nucleotide +9 -0
  60. data/bin/possible_codons_for_this_aminoacid +12 -0
  61. data/bin/random_dna_sequence +12 -0
  62. data/bin/random_sequence +12 -0
  63. data/bin/raw_hamming_distance +12 -0
  64. data/bin/return_longest_substring_via_LCS_algorithm +7 -0
  65. data/bin/reverse_complement +7 -0
  66. data/bin/reverse_sequence +7 -0
  67. data/bin/ruler +12 -0
  68. data/bin/scan_this_input_for_startcodons +12 -0
  69. data/bin/short_aminoacid_letter_from_long_aminoacid_name +7 -0
  70. data/bin/show_atomic_composition +7 -0
  71. data/bin/show_codon_usage +12 -0
  72. data/bin/show_fasta_header +7 -0
  73. data/bin/show_nucleotide_sequence +7 -0
  74. data/bin/show_this_codon_table +7 -0
  75. data/bin/show_this_dna_sequence +7 -0
  76. data/bin/showorf +14 -0
  77. data/bin/simplify_fasta +7 -0
  78. data/bin/sort_aminoacid_based_on_its_hydrophobicity +7 -0
  79. data/bin/split_this_fasta_file_into_chromosomes +7 -0
  80. data/bin/strict_filter_away_invalid_aminoacids +7 -0
  81. data/bin/taxonomy +63 -0
  82. data/bin/three_to_one +7 -0
  83. data/bin/to_rna +7 -0
  84. data/bin/trailing_three_prime +7 -0
  85. data/bin/trypsin_digest +7 -0
  86. data/bin/upcase_this_aminoacid_sequence_and_remove_numbers +7 -0
  87. data/bioroebe.gemspec +97 -0
  88. data/doc/IUPAC_aminoacids_code.md +36 -0
  89. data/doc/IUPAC_nucleotide_code.md +19 -0
  90. data/doc/README.gen +9237 -0
  91. data/doc/blosum.md +5 -0
  92. data/doc/compatibility/BIO_PHP.md +37 -0
  93. data/doc/compatibility/README.md +3 -0
  94. data/doc/compatibility/emboss.md +56 -0
  95. data/doc/extensive_usage_example.md +35 -0
  96. data/doc/german_names_for_the_aminoacids.md +27 -0
  97. data/doc/instructions_for_the_taxonomy_subproject.md +504 -0
  98. data/doc/legacy_paths.md +9 -0
  99. data/doc/pdb_ATOM_entry.md +33 -0
  100. data/doc/quality_control/README.md +2 -0
  101. data/doc/quality_control/commandline_applications.md +13 -0
  102. data/doc/resources.md +23 -0
  103. data/doc/setup.rb +1655 -0
  104. data/doc/statistics/statistics.md +41 -0
  105. data/doc/todo/README.md +5 -0
  106. data/doc/todo/bioroebe_GUI_todo.md +15 -0
  107. data/doc/todo/bioroebe_todo.md +2823 -0
  108. data/doc/using_biomart.md +258 -0
  109. data/html/test.html +144 -0
  110. data/lib/bioroebe/aminoacids/aminoacid_substitution.rb +118 -0
  111. data/lib/bioroebe/aminoacids/aminoacids_mass_table.rb +118 -0
  112. data/lib/bioroebe/aminoacids/codon_percentage.rb +189 -0
  113. data/lib/bioroebe/aminoacids/colourize_hydrophilic_and_hydrophobic_aminoacids.rb +110 -0
  114. data/lib/bioroebe/aminoacids/create_random_aminoacids.rb +221 -0
  115. data/lib/bioroebe/aminoacids/deduce_aminoacid_sequence.rb +460 -0
  116. data/lib/bioroebe/aminoacids/display_aminoacid_table.rb +231 -0
  117. data/lib/bioroebe/aminoacids/show_hydrophobicity.rb +184 -0
  118. data/lib/bioroebe/annotations/README.md +2 -0
  119. data/lib/bioroebe/annotations/create_annotation_format.rb +208 -0
  120. data/lib/bioroebe/autoinclude.rb +7 -0
  121. data/lib/bioroebe/base/base.rb +35 -0
  122. data/lib/bioroebe/base/colours.rb +14 -0
  123. data/lib/bioroebe/base/colours_for_base/colours_for_base.rb +275 -0
  124. data/lib/bioroebe/base/commandline_application/README.md +7 -0
  125. data/lib/bioroebe/base/commandline_application/aminoacids.rb +33 -0
  126. data/lib/bioroebe/base/commandline_application/commandline_application.rb +37 -0
  127. data/lib/bioroebe/base/commandline_application/commandline_arguments.rb +144 -0
  128. data/lib/bioroebe/base/commandline_application/directory.rb +33 -0
  129. data/lib/bioroebe/base/commandline_application/extract.rb +22 -0
  130. data/lib/bioroebe/base/commandline_application/misc.rb +485 -0
  131. data/lib/bioroebe/base/commandline_application/opn.rb +47 -0
  132. data/lib/bioroebe/base/commandline_application/reset.rb +40 -0
  133. data/lib/bioroebe/base/commandline_application/warnings.rb +36 -0
  134. data/lib/bioroebe/base/commandline_application/write_what_into.rb +29 -0
  135. data/lib/bioroebe/base/initialize.rb +18 -0
  136. data/lib/bioroebe/base/misc.rb +94 -0
  137. data/lib/bioroebe/base/namespace.rb +16 -0
  138. data/lib/bioroebe/base/prototype/README.md +12 -0
  139. data/lib/bioroebe/base/prototype/e_and_ee.rb +24 -0
  140. data/lib/bioroebe/base/prototype/misc.rb +108 -0
  141. data/lib/bioroebe/base/prototype/mkdir.rb +20 -0
  142. data/lib/bioroebe/base/prototype/prototype.rb +21 -0
  143. data/lib/bioroebe/base/prototype/reset.rb +26 -0
  144. data/lib/bioroebe/base/reset.rb +11 -0
  145. data/lib/bioroebe/biomart/LICENSE.md +27 -0
  146. data/lib/bioroebe/biomart/attribute.rb +77 -0
  147. data/lib/bioroebe/biomart/biomart.rb +227 -0
  148. data/lib/bioroebe/biomart/database.rb +128 -0
  149. data/lib/bioroebe/biomart/dataset.rb +572 -0
  150. data/lib/bioroebe/biomart/filter.rb +97 -0
  151. data/lib/bioroebe/biomart/server.rb +152 -0
  152. data/lib/bioroebe/blosum/blosum.rb +88 -0
  153. data/lib/bioroebe/calculate/calculate_blosum_score.rb +145 -0
  154. data/lib/bioroebe/calculate/calculate_gc_content.rb +301 -0
  155. data/lib/bioroebe/calculate/calculate_levensthein_distance.rb +100 -0
  156. data/lib/bioroebe/calculate/calculate_melting_temperature.rb +398 -0
  157. data/lib/bioroebe/calculate/calculate_melting_temperature_for_more_than_thirteen_nucleotides.rb +304 -0
  158. data/lib/bioroebe/calculate/calculate_the_position_specific_scoring_matrix.rb +166 -0
  159. data/lib/bioroebe/cell/README.md +1 -0
  160. data/lib/bioroebe/cell/cell.rb +63 -0
  161. data/lib/bioroebe/cleave_and_digest/README.md +2 -0
  162. data/lib/bioroebe/cleave_and_digest/cleave.rb +80 -0
  163. data/lib/bioroebe/cleave_and_digest/digestion.rb +75 -0
  164. data/lib/bioroebe/cleave_and_digest/trypsin.rb +192 -0
  165. data/lib/bioroebe/codon_tables/README.md +9 -0
  166. data/lib/bioroebe/codon_tables/frequencies/287_Pseudomonas_aeruginosa.yml +101 -0
  167. data/lib/bioroebe/codon_tables/frequencies/3702_Arabidopsis_thaliana.yml +77 -0
  168. data/lib/bioroebe/codon_tables/frequencies/4932_Saccharomyces_cerevisiae.yml +103 -0
  169. data/lib/bioroebe/codon_tables/frequencies/7227_Drosophila_melanogaster.yml +71 -0
  170. data/lib/bioroebe/codon_tables/frequencies/83333_Escherichia_coli_K12.yml +103 -0
  171. data/lib/bioroebe/codon_tables/frequencies/9606_Homo_sapiens.yml +123 -0
  172. data/lib/bioroebe/codon_tables/frequencies/9685_Felis_catus.yml +78 -0
  173. data/lib/bioroebe/codon_tables/frequencies/README.md +10 -0
  174. data/lib/bioroebe/codon_tables/frequencies/parse_frequency_table.rb +337 -0
  175. data/lib/bioroebe/codons/README.md +28 -0
  176. data/lib/bioroebe/codons/codon_table.rb +416 -0
  177. data/lib/bioroebe/codons/codon_tables.rb +123 -0
  178. data/lib/bioroebe/codons/codons.rb +517 -0
  179. data/lib/bioroebe/codons/convert_this_codon_to_that_aminoacid.rb +102 -0
  180. data/lib/bioroebe/codons/detect_minimal_codon.rb +180 -0
  181. data/lib/bioroebe/codons/determine_optimal_codons.rb +74 -0
  182. data/lib/bioroebe/codons/possible_codons_for_this_aminoacid.rb +380 -0
  183. data/lib/bioroebe/codons/sanitize_codon_frequency.rb +144 -0
  184. data/lib/bioroebe/codons/show_codon_tables.rb +130 -0
  185. data/lib/bioroebe/codons/show_codon_usage.rb +197 -0
  186. data/lib/bioroebe/codons/show_this_codon_table.rb +573 -0
  187. data/lib/bioroebe/codons/start_codons.rb +105 -0
  188. data/lib/bioroebe/colours/colour_schemes/README.md +10 -0
  189. data/lib/bioroebe/colours/colour_schemes/array_available_colour_schemes.rb +38 -0
  190. data/lib/bioroebe/colours/colour_schemes/buried.rb +70 -0
  191. data/lib/bioroebe/colours/colour_schemes/colour_scheme.rb +101 -0
  192. data/lib/bioroebe/colours/colour_schemes/colour_scheme_demo.rb +262 -0
  193. data/lib/bioroebe/colours/colour_schemes/helix.rb +65 -0
  194. data/lib/bioroebe/colours/colour_schemes/hydropathy.rb +70 -0
  195. data/lib/bioroebe/colours/colour_schemes/nucleotide.rb +47 -0
  196. data/lib/bioroebe/colours/colour_schemes/score.rb +112 -0
  197. data/lib/bioroebe/colours/colour_schemes/simple.rb +42 -0
  198. data/lib/bioroebe/colours/colour_schemes/strand.rb +65 -0
  199. data/lib/bioroebe/colours/colour_schemes/taylor.rb +58 -0
  200. data/lib/bioroebe/colours/colour_schemes/turn.rb +65 -0
  201. data/lib/bioroebe/colours/colour_schemes/zappo.rb +59 -0
  202. data/lib/bioroebe/colours/colourize_sequence.rb +262 -0
  203. data/lib/bioroebe/colours/colours.rb +119 -0
  204. data/lib/bioroebe/colours/misc_colours.rb +80 -0
  205. data/lib/bioroebe/colours/rev.rb +41 -0
  206. data/lib/bioroebe/colours/sdir.rb +21 -0
  207. data/lib/bioroebe/colours/sfancy.rb +21 -0
  208. data/lib/bioroebe/colours/sfile.rb +21 -0
  209. data/lib/bioroebe/colours/simp.rb +21 -0
  210. data/lib/bioroebe/colours/swarn.rb +29 -0
  211. data/lib/bioroebe/colours/use_colours.rb +27 -0
  212. data/lib/bioroebe/configuration/configuration.rb +114 -0
  213. data/lib/bioroebe/configuration/constants.rb +35 -0
  214. data/lib/bioroebe/constants/GUIs.rb +79 -0
  215. data/lib/bioroebe/constants/aminoacids_and_proteins.rb +146 -0
  216. data/lib/bioroebe/constants/base_directory.rb +120 -0
  217. data/lib/bioroebe/constants/carriage_return.rb +14 -0
  218. data/lib/bioroebe/constants/codon_tables.rb +77 -0
  219. data/lib/bioroebe/constants/database_constants.rb +107 -0
  220. data/lib/bioroebe/constants/files_and_directories.rb +579 -0
  221. data/lib/bioroebe/constants/misc.rb +189 -0
  222. data/lib/bioroebe/constants/newline.rb +14 -0
  223. data/lib/bioroebe/constants/nucleotides.rb +114 -0
  224. data/lib/bioroebe/constants/regex.rb +28 -0
  225. data/lib/bioroebe/constants/roebe.rb +38 -0
  226. data/lib/bioroebe/constants/row_terminator.rb +16 -0
  227. data/lib/bioroebe/constants/tabulator.rb +14 -0
  228. data/lib/bioroebe/constants/unicode.rb +12 -0
  229. data/lib/bioroebe/constants/urls.rb +50 -0
  230. data/lib/bioroebe/conversions/README.md +3 -0
  231. data/lib/bioroebe/conversions/convert_aminoacid_to_dna.rb +298 -0
  232. data/lib/bioroebe/conversions/dna_to_aminoacid_sequence.rb +569 -0
  233. data/lib/bioroebe/count/README.md +1 -0
  234. data/lib/bioroebe/count/count_amount_of_aminoacids.rb +352 -0
  235. data/lib/bioroebe/count/count_amount_of_nucleotides.rb +491 -0
  236. data/lib/bioroebe/count/count_at.rb +39 -0
  237. data/lib/bioroebe/count/count_gc.rb +43 -0
  238. data/lib/bioroebe/css/README.md +5 -0
  239. data/lib/bioroebe/css/project.css +121 -0
  240. data/lib/bioroebe/data/README.md +10 -0
  241. data/lib/bioroebe/data/bam/README.md +1 -0
  242. data/lib/bioroebe/data/data.txt +192 -0
  243. data/lib/bioroebe/data/fasta/GFP_mutant_3_coding_sequence.fasta +12 -0
  244. data/lib/bioroebe/data/fasta/alu_elements.fasta +42 -0
  245. data/lib/bioroebe/data/fasta/lady_slippers_orchid.fasta +1197 -0
  246. data/lib/bioroebe/data/fasta/loxP.fasta +2 -0
  247. data/lib/bioroebe/data/fasta/ls_orchid.fasta +1197 -0
  248. data/lib/bioroebe/data/fasta/pax6_in_mouse.fasta +1 -0
  249. data/lib/bioroebe/data/fasta/test.fasta +7 -0
  250. data/lib/bioroebe/data/fasta/test_DNA.fasta +1 -0
  251. data/lib/bioroebe/data/fastq/fastq_example_file.fastq +32 -0
  252. data/lib/bioroebe/data/fastq/fastq_example_file_SP1.fastq +1000 -0
  253. data/lib/bioroebe/data/fastq/one_random_fastq_entry.fastq +4 -0
  254. data/lib/bioroebe/data/genbank/sample_file.genbank +15 -0
  255. data/lib/bioroebe/data/genbank/standard.fasta +3 -0
  256. data/lib/bioroebe/data/gff/Escherichia_coli_K12_plasmid_F_DNA_NC_002483.1.gff3 +345 -0
  257. data/lib/bioroebe/data/gff/sample.gff +2 -0
  258. data/lib/bioroebe/data/gff/test_gene.gff +4 -0
  259. data/lib/bioroebe/data/gff/transcripts.gff +16 -0
  260. data/lib/bioroebe/data/gtf/README.md +1 -0
  261. data/lib/bioroebe/data/json/example_config.json +48 -0
  262. data/lib/bioroebe/data/pdb/1VII.pdb +754 -0
  263. data/lib/bioroebe/data/pdb/ala_phe_ala.pdb +228 -0
  264. data/lib/bioroebe/data/pdb/rcsb_pdb_1VII.fasta +2 -0
  265. data/lib/bioroebe/data/phylip/README.md +11 -0
  266. data/lib/bioroebe/data/phylip/example.phylip +7 -0
  267. data/lib/bioroebe/data/svg/example.svg +301 -0
  268. data/lib/bioroebe/databases/README.md +1 -0
  269. data/lib/bioroebe/databases/download_taxonomy_database.rb +102 -0
  270. data/lib/bioroebe/dotplots/README.md +5 -0
  271. data/lib/bioroebe/dotplots/advanced_dotplot.rb +256 -0
  272. data/lib/bioroebe/dotplots/dotplot.rb +184 -0
  273. data/lib/bioroebe/electron_microscopy/coordinate_analyzer.rb +191 -0
  274. data/lib/bioroebe/electron_microscopy/fix_pos_file.rb +102 -0
  275. data/lib/bioroebe/electron_microscopy/generate_em2em_file.rb +122 -0
  276. data/lib/bioroebe/electron_microscopy/parse_coordinates.rb +197 -0
  277. data/lib/bioroebe/electron_microscopy/read_file_xmd.rb +282 -0
  278. data/lib/bioroebe/electron_microscopy/simple_star_file_generator.rb +131 -0
  279. data/lib/bioroebe/encoding/README.md +2 -0
  280. data/lib/bioroebe/encoding/encoding.rb +45 -0
  281. data/lib/bioroebe/enzymes/README.md +2 -0
  282. data/lib/bioroebe/enzymes/has_this_restriction_enzyme.rb +46 -0
  283. data/lib/bioroebe/enzymes/restriction_enzyme.rb +200 -0
  284. data/lib/bioroebe/enzymes/restriction_enzymes_file.rb +72 -0
  285. data/lib/bioroebe/enzymes/return_restriction_enzyme_sequence_and_cut_position.rb +80 -0
  286. data/lib/bioroebe/enzymes/return_sequence_that_is_cut_via_restriction_enzyme.rb +65 -0
  287. data/lib/bioroebe/enzymes/show_restriction_enzymes.rb +119 -0
  288. data/lib/bioroebe/exceptions/README.md +2 -0
  289. data/lib/bioroebe/exceptions/exceptions.rb +17 -0
  290. data/lib/bioroebe/ext/LICENCE.md +5 -0
  291. data/lib/bioroebe/ext/README.md +7 -0
  292. data/lib/bioroebe/ext/main.cpp +45 -0
  293. data/lib/bioroebe/ext/nucleotide.cpp +24 -0
  294. data/lib/bioroebe/ext/nussinov_algorithm.cpp +348 -0
  295. data/lib/bioroebe/ext/sequence +0 -0
  296. data/lib/bioroebe/ext/sequence.cpp +162 -0
  297. data/lib/bioroebe/fasta_and_fastq/README.md +6 -0
  298. data/lib/bioroebe/fasta_and_fastq/autocorrect_the_name_of_this_fasta_file.rb +88 -0
  299. data/lib/bioroebe/fasta_and_fastq/compact_fasta_file/compact_fasta_file.rb +151 -0
  300. data/lib/bioroebe/fasta_and_fastq/display_how_many_fasta_entries_are_in_this_directory.rb +111 -0
  301. data/lib/bioroebe/fasta_and_fastq/download_fasta.rb +248 -0
  302. data/lib/bioroebe/fasta_and_fastq/fasta_defline/README.md +2 -0
  303. data/lib/bioroebe/fasta_and_fastq/fasta_defline/fasta_defline.rb +113 -0
  304. data/lib/bioroebe/fasta_and_fastq/fasta_parser.rb +167 -0
  305. data/lib/bioroebe/fasta_and_fastq/fastq_format_explainer.rb +131 -0
  306. data/lib/bioroebe/fasta_and_fastq/length_modifier/length_modifier.rb +87 -0
  307. data/lib/bioroebe/fasta_and_fastq/parse_fasta/constants.rb +50 -0
  308. data/lib/bioroebe/fasta_and_fastq/parse_fasta/initialize.rb +86 -0
  309. data/lib/bioroebe/fasta_and_fastq/parse_fasta/menu.rb +117 -0
  310. data/lib/bioroebe/fasta_and_fastq/parse_fasta/misc.rb +981 -0
  311. data/lib/bioroebe/fasta_and_fastq/parse_fasta/parse_fasta.rb +27 -0
  312. data/lib/bioroebe/fasta_and_fastq/parse_fasta/report.rb +156 -0
  313. data/lib/bioroebe/fasta_and_fastq/parse_fasta/reset.rb +128 -0
  314. data/lib/bioroebe/fasta_and_fastq/parse_fasta/run.rb +20 -0
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  603. data/lib/bioroebe/toplevel_methods/cliner.rb +81 -0
  604. data/lib/bioroebe/toplevel_methods/complement.rb +58 -0
  605. data/lib/bioroebe/toplevel_methods/convert_global_env.rb +39 -0
  606. data/lib/bioroebe/toplevel_methods/databases.rb +73 -0
  607. data/lib/bioroebe/toplevel_methods/delimiter.rb +19 -0
  608. data/lib/bioroebe/toplevel_methods/digest.rb +71 -0
  609. data/lib/bioroebe/toplevel_methods/download_and_fetch_data.rb +146 -0
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  625. data/lib/bioroebe/toplevel_methods/map_ncbi_entry_to_eutils_id.rb +88 -0
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  661. data/lib/bioroebe/utility_scripts/determine_missing_nucleotides_percentage.rb +137 -0
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  674. data/lib/bioroebe/utility_scripts/parse_taxonomy.rb +168 -0
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  677. data/lib/bioroebe/utility_scripts/punnet/punnet.rb +126 -0
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  681. data/lib/bioroebe/utility_scripts/showorf/initialize.rb +52 -0
  682. data/lib/bioroebe/utility_scripts/showorf/menu.rb +68 -0
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  684. data/lib/bioroebe/utility_scripts/showorf/run.rb +152 -0
  685. data/lib/bioroebe/utility_scripts/showorf/show.rb +97 -0
  686. data/lib/bioroebe/utility_scripts/showorf/showorf.rb +488 -0
  687. data/lib/bioroebe/version/version.rb +44 -0
  688. data/lib/bioroebe/viennarna/README.md +3 -0
  689. data/lib/bioroebe/viennarna/rnafold_wrapper.rb +196 -0
  690. data/lib/bioroebe/with_gui.rb +18 -0
  691. data/lib/bioroebe/www/bioroebe.cgi +44 -0
  692. data/lib/bioroebe/www/embeddable_interface.rb +686 -0
  693. data/lib/bioroebe/www/sinatra/sinatra.rb +1013 -0
  694. data/lib/bioroebe/yaml/agarose/agarose_concentrations.yml +21 -0
  695. data/lib/bioroebe/yaml/aminoacids/amino_acids.yml +92 -0
  696. data/lib/bioroebe/yaml/aminoacids/amino_acids_abbreviations.yml +31 -0
  697. data/lib/bioroebe/yaml/aminoacids/amino_acids_average_mass_table.yml +33 -0
  698. data/lib/bioroebe/yaml/aminoacids/amino_acids_classification.yml +18 -0
  699. data/lib/bioroebe/yaml/aminoacids/amino_acids_english.yml +26 -0
  700. data/lib/bioroebe/yaml/aminoacids/amino_acids_frequency.yml +44 -0
  701. data/lib/bioroebe/yaml/aminoacids/amino_acids_long_name_to_one_letter.yml +61 -0
  702. data/lib/bioroebe/yaml/aminoacids/amino_acids_molecular_formula.yml +32 -0
  703. data/lib/bioroebe/yaml/aminoacids/amino_acids_monoisotopic_mass_table.yml +38 -0
  704. data/lib/bioroebe/yaml/aminoacids/amino_acids_reste.yml +35 -0
  705. data/lib/bioroebe/yaml/aminoacids/amino_acids_three_to_one.yml +34 -0
  706. data/lib/bioroebe/yaml/aminoacids/hydropathy_table.yml +44 -0
  707. data/lib/bioroebe/yaml/aminoacids/molecular_weight.yml +29 -0
  708. data/lib/bioroebe/yaml/aminoacids/simple_aminoacids.yml +66 -0
  709. data/lib/bioroebe/yaml/aminoacids/weight_of_common_proteins.yml +33 -0
  710. data/lib/bioroebe/yaml/antisense/antisense.yml +9 -0
  711. data/lib/bioroebe/yaml/base_composition_of_dna.yml +37 -0
  712. data/lib/bioroebe/yaml/blosum/blosum45.yml +36 -0
  713. data/lib/bioroebe/yaml/blosum/blosum50.yml +34 -0
  714. data/lib/bioroebe/yaml/blosum/blosum62.yml +35 -0
  715. data/lib/bioroebe/yaml/blosum/blosum80.yml +37 -0
  716. data/lib/bioroebe/yaml/blosum/blosum90.yml +36 -0
  717. data/lib/bioroebe/yaml/blosum/blosum_matrix.yml +200 -0
  718. data/lib/bioroebe/yaml/chromosomes/chromosome_numbers.yml +30 -0
  719. data/lib/bioroebe/yaml/codon_tables/1.yml +113 -0
  720. data/lib/bioroebe/yaml/codon_tables/10.yml +89 -0
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  725. data/lib/bioroebe/yaml/codon_tables/15.yml +94 -0
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  727. data/lib/bioroebe/yaml/codon_tables/2.yml +86 -0
  728. data/lib/bioroebe/yaml/codon_tables/21.yml +89 -0
  729. data/lib/bioroebe/yaml/codon_tables/22.yml +89 -0
  730. data/lib/bioroebe/yaml/codon_tables/23.yml +91 -0
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  735. data/lib/bioroebe/yaml/codon_tables/28.yml +97 -0
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  737. data/lib/bioroebe/yaml/codon_tables/3.yml +98 -0
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  740. data/lib/bioroebe/yaml/codon_tables/33.yml +101 -0
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  747. data/lib/bioroebe/yaml/configuration/browser.yml +1 -0
  748. data/lib/bioroebe/yaml/configuration/colourize_fasta_sequences.yml +14 -0
  749. data/lib/bioroebe/yaml/configuration/default_colours_for_the_aminoacids.yml +28 -0
  750. data/lib/bioroebe/yaml/configuration/temp_dir.yml +1 -0
  751. data/lib/bioroebe/yaml/configuration/try_to_use_matplotlib.yml +1 -0
  752. data/lib/bioroebe/yaml/configuration/use_opn.yml +1 -0
  753. data/lib/bioroebe/yaml/configuration/use_this_database.yml +1 -0
  754. data/lib/bioroebe/yaml/create_these_directories_on_startup/create_these_directories_on_startup.yml +9 -0
  755. data/lib/bioroebe/yaml/default_dna_input.yml +3 -0
  756. data/lib/bioroebe/yaml/enzymes/enzyme_classes.yml +15 -0
  757. data/lib/bioroebe/yaml/enzymes/pH-Optima.yml +11 -0
  758. data/lib/bioroebe/yaml/fasta_and_fastq/fastq_quality_schemes.yml +44 -0
  759. data/lib/bioroebe/yaml/genomes/README.md +16 -0
  760. data/lib/bioroebe/yaml/humans/README.md +2 -0
  761. data/lib/bioroebe/yaml/humans/human_chromosomes.yml +53 -0
  762. data/lib/bioroebe/yaml/laboratory/README.md +1 -0
  763. data/lib/bioroebe/yaml/laboratory/pipettes.yml +8 -0
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  765. data/lib/bioroebe/yaml/nuclear_localization_sequences.yml +15 -0
  766. data/lib/bioroebe/yaml/nucleotides/abbreviations_for_nucleotides.yml +29 -0
  767. data/lib/bioroebe/yaml/nucleotides/nucleotide_density.yml +10 -0
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  771. data/lib/bioroebe/yaml/pathways/citric_acid_cycle.yml +16 -0
  772. data/lib/bioroebe/yaml/pathways/glycolysis.yml +20 -0
  773. data/lib/bioroebe/yaml/pathways/shikimate_pathway.yml +23 -0
  774. data/lib/bioroebe/yaml/pathways/urea_cycle.yml +11 -0
  775. data/lib/bioroebe/yaml/primers/README.md +4 -0
  776. data/lib/bioroebe/yaml/primers/primers.yml +3 -0
  777. data/lib/bioroebe/yaml/promoters/35S.yml +15 -0
  778. data/lib/bioroebe/yaml/promoters/strong_promoters.yml +24 -0
  779. data/lib/bioroebe/yaml/proteases/proteases.yml +3 -0
  780. data/lib/bioroebe/yaml/proteins/ubiquitin.yml +4 -0
  781. data/lib/bioroebe/yaml/remote_urls/README.md +4 -0
  782. data/lib/bioroebe/yaml/remote_urls/remote_urls.yml +3 -0
  783. data/lib/bioroebe/yaml/restriction_enzymes/restriction_enzymes.yml +630 -0
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  785. data/lib/bioroebe/yaml/sequences/README.md +2 -0
  786. data/lib/bioroebe/yaml/talens.yml +22 -0
  787. data/lib/bioroebe/yaml/viruses/ecoli_phages.yml +63 -0
  788. data/lib/bioroebe/yaml/viruses/viruses.yml +6 -0
  789. data/lib/bioroebe.rb +5 -0
  790. data/spec/testing_toplevel_method_editor.rb +20 -0
  791. data/spec/testing_toplevel_method_url.rb +15 -0
  792. data/spec/testing_toplevel_method_verbose.rb +13 -0
  793. data/test/advanced_svg_example.rb +307 -0
  794. data/test/testing_bioroebe.rb +25 -0
  795. data/test/testing_codons.rb +45 -0
  796. data/test/testing_dna_to_rna_conversion.rb +15 -0
  797. data/test/testing_parse_pdb_file.rb +23 -0
  798. data/test/testing_reverse_complement.rb +32 -0
  799. data/test/testing_svg_component_of_bioroebe.rb +311 -0
  800. data/test/testing_svg_component_of_bioroebe_from_json_dataset.rb +34 -0
  801. data/test/testing_taxonomy.rb +22 -0
  802. metadata +1059 -0
@@ -0,0 +1,111 @@
1
+ #!/usr/bin/ruby -w
2
+ # Encoding: UTF-8
3
+ # frozen_string_literal: true
4
+ # =========================================================================== #
5
+ # === Bioroebe::SVG::MiniFeature
6
+ #
7
+ # A Bioroebe::SVG::MiniFeature object represents a single genomic feature
8
+ # (e.g. a gene, transcript, exon, start codon, etc), it is a lightweight
9
+ # object that holds the minimum information necessary to do the render
10
+ # operation.
11
+ # =========================================================================== #
12
+ module Bioroebe
13
+
14
+ class SVG
15
+
16
+ class MiniFeature # === Bioroebe::SVG::MiniFeature
17
+
18
+ attr_accessor :id
19
+ attr_accessor :start
20
+ attr_accessor :end
21
+ attr_accessor :strand
22
+ attr_accessor :exons
23
+ attr_accessor :utrs
24
+ attr_accessor :block_gaps
25
+ attr_accessor :segment_height
26
+ attr_accessor :params
27
+
28
+ # ========================================================================= #
29
+ # === initialize
30
+ #
31
+ # Creates a new MiniFeature.
32
+ #
33
+ # Arguments:
34
+ #
35
+ # :start = the start position of the feature
36
+ # :end = the end position of the feature
37
+ # :strand = the strand of the feature
38
+ # :exons = an array of exon positions
39
+ # :utrs = an array of utrs positions
40
+ # :block_gaps = an array of regions with nothing to be drawn, e.g. introns
41
+ # :id = the name for the feature such as the gene name or transcript ID
42
+ # :segment_height = the height of the current feature
43
+ #
44
+ # Usage examples:
45
+ #
46
+ # mini1 = Bioroebe::SVG::MiniFeature.new(
47
+ # :start=>3631,
48
+ # :end=>5899,
49
+ # :strand=>'+',
50
+ # :exons=>[4000, 4500, 4700, 5000],
51
+ # :utrs=>[3631, 3650],
52
+ # :segment_height=>5,
53
+ # :id=>"AT1G01010"
54
+ # )
55
+ #
56
+ # === MiniFeatures and Tracks
57
+ #
58
+ # MiniFeatures are created and added to Bioroebe::SVG::Track# objects
59
+ # which will take responsibility for positioning and syling them.
60
+ # ========================================================================= #
61
+ def initialize(i)
62
+ # ======================================================================= #
63
+ # Deconstruct from the input hash given.
64
+ # ======================================================================= #
65
+
66
+ # ======================================================================= #
67
+ # === @start
68
+ # ======================================================================= #
69
+ @start = i[:start]
70
+ # ======================================================================= #
71
+ # === @end
72
+ # ======================================================================= #
73
+ @end = i[:end]
74
+ # ======================================================================= #
75
+ # === @strand
76
+ # ======================================================================= #
77
+ @strand = i[:strand]
78
+ # ======================================================================= #
79
+ # === @exons
80
+ # ======================================================================= #
81
+ @exons = i[:exons] || []
82
+ # ======================================================================= #
83
+ # === @utrs
84
+ # ======================================================================= #
85
+ @utrs = i[:utrs] || [] #start, ennd, strand, arg[:exons], arg[:utrs]
86
+ # ======================================================================= #
87
+ # === @id
88
+ # ======================================================================= #
89
+ @id = i[:id]
90
+ # ======================================================================= #
91
+ # === @params
92
+ # ======================================================================= #
93
+ @params = i[:params]
94
+ # ======================================================================= #
95
+ # === @segment_height
96
+ # ======================================================================= #
97
+ @segment_height = i[:segment_height]
98
+ reset
99
+ end
100
+
101
+ # ========================================================================= #
102
+ # === reset (reset tag)
103
+ # ========================================================================= #
104
+ def reset
105
+ # ======================================================================= #
106
+ # === @block_gaps
107
+ # ======================================================================= #
108
+ @block_gaps = []
109
+ end
110
+
111
+ end; end; end
@@ -0,0 +1,570 @@
1
+ #!/usr/bin/ruby -w
2
+ # Encoding: UTF-8
3
+ # frozen_string_literal: true
4
+ # =========================================================================== #
5
+ # === Bioroebe::SVG::Page
6
+ #
7
+ # The Bioroebe::SVG::Page class represents the page of the final,
8
+ # rendered SVG. It thus acts as the "top level container", into
9
+ # which individual Bioroebe::SVG::Tracks are added.
10
+ #
11
+ # It will contain a scale and all the tracks along with their features.
12
+ #
13
+ # The scale to be calculated, will start at the genomic co-ordinates
14
+ # of the start of the first feature and stop at the end of the last
15
+ # feature.
16
+ # =========================================================================== #
17
+ # === Setting up a new Page object:
18
+ #
19
+ # This can be done like in this way:
20
+ #
21
+ # page = Bioroebe::SVG::Page.new(
22
+ # :width => 1024,
23
+ # :height => 200,
24
+ # :number_of_intervals => 10
25
+ # )
26
+ # =========================================================================== #
27
+ module Bioroebe
28
+
29
+ class SVG
30
+
31
+ class Page # === Bioroebe::SVG::Page
32
+
33
+ # ========================================================================= #
34
+ # === initialize
35
+ #
36
+ # Creates a new Page object.
37
+ #
38
+ # Arguments:
39
+ #
40
+ # height: this is the minimum height of the rendered page.
41
+ # width: this is the minimum width of the rendered page.
42
+ # background_color: this is the background color of the page
43
+ # (default is none), can be any SVG colour
44
+ # eg rgb(256,0,0) or #FF0000.
45
+ #
46
+ # ========================================================================= #
47
+ def initialize(i)
48
+ @height = i[:height]
49
+ @width = i[:width]
50
+ i[:style] = "background-color:#{i[:background_color]};" if i[:background_color]
51
+ # ======================================================================= #
52
+ # === @svg
53
+ #
54
+ # Instantiate a new SVGEE object next.
55
+ # ======================================================================= #
56
+ @svg = SVGEE.new(i)
57
+ @num_intervals = i[:number_of_intervals]
58
+ @svg.update_height(@height)
59
+ reset
60
+ end
61
+
62
+ # ========================================================================= #
63
+ # === reset (reset tag)
64
+ # ========================================================================= #
65
+ def reset
66
+ # ======================================================================= #
67
+ # === @track_top
68
+ # ======================================================================= #
69
+ @track_top = 30
70
+ # ======================================================================= #
71
+ # === @tracks
72
+ # ======================================================================= #
73
+ @tracks = [] # Array of track objects with loads of features in it.
74
+ @scale_start = 1.0/0.0
75
+ @scale_stop = -1.0/0.0
76
+ @nt_per_percent = 1
77
+ end
78
+
79
+ # ========================================================================= #
80
+ # === Bioroebe::SVG::Page.from_json
81
+ #
82
+ # Takes a custom-written json file and uses it to generate an SVG
83
+ # document based on the information in that page.
84
+ #
85
+ #* :json a JSON file describing the parameters and files needed to build
86
+ # an SVG document
87
+ # ========================================================================= #
88
+ def self.from_json(args)
89
+ require 'json'
90
+ data = JSON.parse(File.open(args[:json], 'r').read)
91
+ p = Page.new(:width => data['Page']['width'],
92
+ :height => data['Page']['height'],
93
+ :number_of_intervals => data['Page']['intervals'])
94
+ data['Tracks'].each { |track|
95
+ # Prepare the tracks-arguments.
96
+ track_args = track.dup
97
+ track_args.delete('file')
98
+ track_args.delete('file_type')
99
+ track_args = track_args.inject({}) { |memo, (k, v)| memo[k.to_sym] = v; memo }
100
+ # ===================================================================== #
101
+ # Convert any of the pre-made gradient strings in the keys
102
+ # to symbol.
103
+ # ===================================================================== #
104
+ track_args.each_key { |k|
105
+ next unless track_args[k].respond_to?(:to_sym)
106
+ track_args[k] = track_args[k].to_sym if Glyph.gradients.include?(track_args[k].to_sym)
107
+ }
108
+ svg_track = p.add_track(track_args)
109
+ features = [] ##set up the features...
110
+ case track['file_type'] # Deal with the gff and data files.
111
+ # ===================================================================== #
112
+ # === gff
113
+ # ===================================================================== #
114
+ when 'gff'
115
+ groups = {}
116
+ parentless_features = []
117
+ Page.parse_gff(track['file']).each { |gff| #gets features in a list and links their children to them as members of the array at the key
118
+ parent_id = gff.attributes.select { |a| a.first == 'Parent' }
119
+ if parent_id.empty?
120
+ parentless_features << gff
121
+ else
122
+ if groups[parent_id.first.last].nil?
123
+ groups[parent_id.first.last] = []
124
+ groups[parent_id.first.last] << gff
125
+ else
126
+ groups[parent_id.first.last] << gff
127
+ end
128
+ end
129
+ }
130
+ # =================================================================== #
131
+ # Now flick through the parentless features and add any
132
+ # exons or UTRs.
133
+ # =================================================================== #
134
+ parentless_features.each { |plf|
135
+ gff_id = plf.attributes.select { |a| a.first == 'ID' }
136
+ gff_id = gff_id.first.last
137
+ exons = []
138
+ utrs = []
139
+ children = groups[gff_id] || children = []
140
+ children.each { |child|
141
+ case child.feature
142
+ when 'exon','CDS'
143
+ exons += [child.start, child.end]
144
+ when /utr/i # Handle UTRs here.
145
+ utrs += [child.start, child.end]
146
+ end
147
+ }
148
+ features << MiniFeature.new(
149
+ :start => plf.start,
150
+ :end => plf.end,
151
+ :exons => exons,
152
+ :utrs => utrs,
153
+ :strand => plf.strand,
154
+ :id => gff_id
155
+ )
156
+ } # parentless features end
157
+ # ===================================================================== #
158
+ # === data
159
+ # ===================================================================== #
160
+ when 'data'
161
+ # =================================================================== #
162
+ # Each line is a data feature.
163
+ # =================================================================== #
164
+ File.open(track['file'], 'r').each { |line|
165
+ start, stop, value = line.split(/\t/)
166
+ features << MiniFeature.new(
167
+ :start => start.to_i,
168
+ :end => stop.to_i,
169
+ :segment_height => value.to_f
170
+ )
171
+ }
172
+ end
173
+ features.each { |f| svg_track.add(f) }
174
+ } # track end
175
+ p.write(args[:outfile])
176
+ end
177
+
178
+ # ========================================================================= #
179
+ # === add_track
180
+ #
181
+ # Adds a new Bioroebe::SVG::Track object to the current Bioroebe::SVG::Page
182
+ # object.
183
+ #
184
+ #==args
185
+ #
186
+ #* :glyph = one of Bioroebe::SVG::Glyphs#glyphs currently
187
+ # [:generic, :directed, :transcript, :scale, :label, :histogram, :circle, :down_triangle, :up_triangle, :span]
188
+ #* :stroke_color = the outline colour of the glyphs in the track (default = "black"), can be any SVG colour eg rgb(256,0,0) or #FF0000
189
+ #* :fill_color = the fill colour of the glyphs in the track (default = 'red'), can be any SVG colour eg rgb(256,0,0) or #FF0000, or one of the built in gradient types Bioroebe::SVG::Glyph#gradients
190
+ # [:red_white_h, :green_white_h, :blue_white_h, :yellow_white_h, :red_white_radial, :green_white_radial, :blue_white_radial, :yellow_white_radial ]
191
+ # or a custom definition of a gradient.
192
+ #
193
+ # {:type => :radial,
194
+ # :id => :custom,
195
+ # :cx => 5,
196
+ # :cy => 5,
197
+ # :r => 50,
198
+ # :fx => 50,
199
+ # :fy => 50,
200
+ # :stops => [ {
201
+ # :offset => 0,
202
+ # :color => 'rgb(255,255,255)',
203
+ # :opacity => 0
204
+ # }, {
205
+ # :offset => 100,
206
+ # :color => 'rgb(0,127,200)',
207
+ # :opacity => 1
208
+ # }, ]
209
+ # }
210
+ #
211
+ # * :track_height = minimum height for the track, will be modified automatically if more space is needed e.g for overlapping features (default = auto),
212
+ # * :name = a displayed name for the track (default = 'feature_track')
213
+ # * :label = display the name given to the track (default = true),
214
+ # * :stroke_width = width in pixels of the outline of the glyphs (default=1)
215
+ # * :x_round = x radius of the ellipse used to round off the corners of rectangles (default = 1)
216
+ # * :y_round = y radius of the ellipse used to round off the corners of rectangles (default = 1)
217
+ # :utr_fill_color = the fill colour of the utr part of the glyph (default = 'black'), can be any SVG colour eg rgb(256,0,0) or #FF0000, or one of the built in gradient types Bioroebe::SVG::Glyph#gradients
218
+ # [:red_white_h, :green_white_h, :blue_white_h, :yellow_white_h, :red_white_radial, :green_white_radial, :blue_white_radial, :yellow_white_radial ]
219
+ #
220
+ # or a custom definition of a gradient
221
+ #
222
+ # {:type => :radial,
223
+ # :id => :custom,
224
+ # :cx => 5,
225
+ # :cy => 5,
226
+ # :r => 50,
227
+ # :fx => 50,
228
+ # :fy => 50,
229
+ # :stops => [ {
230
+ # :offset => 0,
231
+ # :color => 'rgb(255,255,255)',
232
+ # :opacity => 0
233
+ # }, {
234
+ # :offset => 100,
235
+ # :color => 'rgb(0,127,200)',
236
+ # :opacity => 1
237
+ # }, ]
238
+ # }
239
+ #* :utr_stroke = the outline colour of the utr part of the glyph (default = "black"), can be any SVG colour eg rgb(256,0,0) or #FF0000
240
+ #* :utr_stroke_width = The width of the outline stroke for the utr part of the glyph (default = 1)
241
+ #* :exon_fill_color = the fill colour of the utr part of the glyph (default = 'red'), can be any SVG colour eg rgb(256,0,0) or #FF0000, or one of the built in gradient types Bioroebe::SVG::Glyph#gradients or a custom definition of a gradient
242
+ # [:red_white_h, :green_white_h, :blue_white_h, :yellow_white_h, :red_white_radial, :green_white_radial, :blue_white_radial, :yellow_white_radial ]
243
+ #or a custom definition of a gradient
244
+ # {:type => :radial,
245
+ # :id => :custom,
246
+ # :cx => 5,
247
+ # :cy => 5,
248
+ # :r => 50,
249
+ # :fx => 50,
250
+ # :fy => 50,
251
+ # :stops => [ {
252
+ # :offset => 0,
253
+ # :color => 'rgb(255,255,255)',
254
+ # :opacity => 0
255
+ # }, {
256
+ # :offset => 100,
257
+ # :color => 'rgb(0,127,200)',
258
+ # :opacity => 1
259
+ # }, ]
260
+ #
261
+ # :exon_stroke = the outline colour of the exon part of the glyph
262
+ # (default = "black") can be any SVG colour eg rgb(256,0,0) or #FF0000
263
+ # :exon_stroke_width = The width of the outline stroke for the exon part
264
+ # of the glyph (default = 1)
265
+ # :line_color = the colour for the line part that joins the blocks (default = 'black') can be any SVG colour eg rgb(256,0,0) or #FF0000
266
+ # :line_width = the width ffor the line part that joins the blocks (default = 1)
267
+ # :exon_style = an arbitrary SVG compliant style string eg "fill-opacity:0.4;"
268
+ # :utr_style = an arbitrary SVG compliant style string eg "fill-opacity:0.4;"
269
+ # :line_style = an arbitrary SVG compliant style string eg "fill-opacity:0.4;"
270
+ # :gap_marker = style of the line between blocks - either angled or straight
271
+ #
272
+ # Will return:
273
+ #
274
+ # a new Bioroebe::SVG::Track object
275
+ #
276
+ # ========================================================================= #
277
+ def add_track(args)
278
+ # ======================================================================= #
279
+ # Sort out the colour/gradient options
280
+ # ======================================================================= #
281
+ [:fill_color, :exon_fill_color, :utr_fill_color].each { |colour_tag|
282
+ if Glyph.gradients.include?(args[colour_tag])
283
+ @svg.gradient(Glyph.gradient(args[colour_tag]))
284
+ args[colour_tag] = "url(##{args[colour_tag]})"
285
+ elsif args[colour_tag].instance_of?(Hash)
286
+ @svg.gradient(args[colour_tag])
287
+ args[colour_tag] = "url(##{args[colour_tag][:id]})"
288
+ end
289
+ }
290
+ @tracks << Track.new(args)
291
+ return @tracks.last
292
+ end
293
+
294
+ # ========================================================================= #
295
+ # === get_limits
296
+ #
297
+ # Calculates the Page scale-start and scale-stop position and the
298
+ # nucleotides per pixel for the current Page.
299
+ # ========================================================================= #
300
+ def get_limits
301
+ @tracks.each { |track|
302
+ lowest = track.features.sort { |x, y|
303
+ x.start <=> y.start
304
+ }.first.start
305
+ highest = track.features.sort { |x, y| y.end <=> x.end }.first.end
306
+ @scale_start = lowest if lowest < @scale_start
307
+ @scale_stop = highest if highest > @scale_stop
308
+ @nt_per_px_x = (@scale_stop - @scale_start).to_f / @width.to_f
309
+ }
310
+ begin
311
+ old_nt_per_px_x = @nt_per_px_x
312
+ @tracks.each { |track|
313
+ highest = track.features.map { |feat|
314
+ compute_boundaries(feat)[1]
315
+ }.max
316
+ if highest > @scale_stop
317
+ @scale_stop = highest
318
+ @nt_per_px_x = (@scale_stop - @scale_start).to_f / @width.to_f
319
+ end
320
+ }
321
+ end while (@nt_per_px_x - old_nt_per_px_x).abs > 1
322
+ end
323
+
324
+ # ========================================================================= #
325
+ # === compute_boundaries
326
+ # ========================================================================= #
327
+ def compute_boundaries(feature)
328
+ feat_end = feature.end
329
+ feat_end += (8 * @nt_per_px_x * feature.id.to_s.length).to_i if feature.id and @nt_per_px_x
330
+ [feature.start, feat_end]
331
+ end
332
+
333
+ # ========================================================================= #
334
+ # === draw_scale
335
+ #
336
+ # Adds scale bar to the list of objects to be rendered in the final.
337
+ # ========================================================================= #
338
+ def draw_scale
339
+ Glyph.scale(:start => @scale_start,
340
+ :stop => @scale_stop,
341
+ :number_of_intervals => @num_intervals,
342
+ :page_width => @width).each { |entry| @svg.add_primitive(entry) }
343
+ end
344
+
345
+ # ========================================================================= #
346
+ # === Page.parse_gff
347
+ #
348
+ # This method will parse a GFF file into an Array of
349
+ # Bio::GFF::GFF3::Record[http://bioruby.org/rdoc/Bio/GFF/GFF3/Record.html] objects
350
+ #* gff_file - a GFF-formatted file
351
+ #* returns - an Array of Bio::GFF::GFF3::Record[http://bioruby.org/rdoc/Bio/GFF/GFF3/Record.html] objects
352
+ # ========================================================================= #
353
+ def self.parse_gff(
354
+ gff_file = HOME_DIRECTORY_OF_USER_X+'DATA/PROGRAMMING_LANGUAGES/RUBY/src/bioroebe/test/test_gene.gff'
355
+ )
356
+ require 'bio' # This depends on 'bio' gem for now, unortunately.
357
+ a = []
358
+ File.open(gff_file).each { |line|
359
+ next if line =~ /^#/
360
+ a << Bio::GFF::GFF3::Record.new(line)
361
+ }
362
+ a
363
+ end
364
+
365
+ # ========================================================================= #
366
+ # === draw_label
367
+ #
368
+ # Adds the Bioroebe::SVG::Primitive objects to the SVGEE object
369
+ #* +args+ - an Array of Bioroebe::SVG::Primitive object
370
+ # ========================================================================= #
371
+ def draw_label(args)
372
+ Glyph.label(:text => args[:text],
373
+ :x => args[:x],
374
+ :y => args[:y]).each { |g| @svg.add_primitive(g) }
375
+ end
376
+
377
+ # ========================================================================= #
378
+ # === draw_generic
379
+ # ========================================================================= #
380
+ def draw_generic(args) #remember presentation info comes from track@args when the track is defined
381
+ Glyph.generic(args).each { |g| @svg.add_primitive(g) }
382
+ end
383
+
384
+ # ========================================================================= #
385
+ # === draw_directed
386
+ # ========================================================================= #
387
+ def draw_directed(args)
388
+ Glyph.directed(args).each { |g| @svg.add_primitive(g) }
389
+ end
390
+
391
+ # ========================================================================= #
392
+ # === draw_circle
393
+ # ========================================================================= #
394
+ def draw_circle(args)
395
+ Glyph.circle(args).each { |g| @svg.add_primitive(g) }
396
+ end
397
+
398
+ # ========================================================================= #
399
+ # === draw_transcript
400
+ # ========================================================================= #
401
+ def draw_transcript(args)
402
+ Glyph.transcript(args).each { |g| @svg.add_primitive(g) }
403
+ end
404
+
405
+ # ========================================================================= #
406
+ # === draw_histogram
407
+ # ========================================================================= #
408
+ def draw_histogram(args)
409
+ Glyph.generic(args).each { |g| @svg.add_primitive(g) }
410
+ end
411
+
412
+ # ========================================================================= #
413
+ # === draw_up_triangle
414
+ # ========================================================================= #
415
+ def draw_up_triangle(args)
416
+ Glyph.up_triangle(args).each { |g| @svg.add_primitive(g) }
417
+ end
418
+
419
+ # ========================================================================= #
420
+ # === draw_down_triangle
421
+ # ========================================================================= #
422
+ def draw_down_triangle(args)
423
+ Glyph.down_triangle(args).each { |g| @svg.add_primitive(g) }
424
+ end
425
+
426
+ # ========================================================================= #
427
+ # === draw_span
428
+ # ========================================================================= #
429
+ def draw_span(args)
430
+ Glyph.span(args).each { |g|
431
+ @svg.add_primitive(g)
432
+ }
433
+ end
434
+
435
+ # ========================================================================= #
436
+ # === draw_features
437
+ #
438
+ # Takes a Bioroebe::SVG::Track object and does the work to draw features.
439
+ #
440
+ # It examines the the type of track and calculates the required
441
+ # parameters * +args+ - an Array of Bioroebe::SVG::Track object
442
+ # ========================================================================= #
443
+ def draw_features(track)
444
+ if [:histogram, 'histogram'].include?(track.glyph) #do different stuff for data tracks...
445
+ y = @track_top + (track.track_height)
446
+ max = track.max_y ? track.max_y : track.features.sort { |a, b| a.segment_height <=> b.segment_height }.last.segment_height
447
+ min = 0
448
+ if track.label
449
+ draw_label(:text => track.name, :y => @track_top += 30, :x => 3)
450
+ end
451
+ draw_label(:text => max, :x => to_px(@scale_stop - @scale_start) + 5, :y => @track_top + 5)
452
+ draw_label(:text => min, :x => to_px(@scale_stop - @scale_start) + 5, :y => @track_top + track.track_height + 5)
453
+ data_interval = max - min
454
+ data_per_px = track.track_height.to_f / data_interval.to_f
455
+ track.features.each do |f|
456
+ x = to_px(f.start - @scale_start)
457
+ width = to_px((f.end - @scale_start) - (f.start - @scale_start))
458
+ height = f.segment_height.to_f * data_per_px
459
+ y = @track_top + track.track_height - height + 5
460
+ #$stderr.puts f.segment_height, data_per_px, data_interval, max, min, "------"
461
+ self.send("draw_#{track.glyph}", {:x => x,
462
+ :y => y,
463
+ :width => width,
464
+ :height => height}.merge!(track.args))
465
+ end
466
+ @track_top += (track.track_height) + 20
467
+ else ##following stuff for the features
468
+ if track.label
469
+ draw_label(:text => track.name, :y => @track_top += 30, :x => 3)
470
+ end
471
+ track.get_rows(self) ##work out how many rows and which features belong in each row...
472
+ track.features.each_with_index do |f, index|
473
+ x = to_px(f.start - @scale_start) #bottom left of feature
474
+ all_sub_blocks = []
475
+ #convert the exon and utr start stops to px start stops and px widths
476
+ exons = []
477
+ if f.exons
478
+ f.exons.each_slice(2).each { |exon|
479
+ all_sub_blocks << exon
480
+ next if exon.nil?
481
+ exons << [to_px(exon[0] - @scale_start), to_px((exon[1] - @scale_start) - (exon[0] - @scale_start))]
482
+ }
483
+ end
484
+ f.exons = exons
485
+ utrs = []
486
+ if f.utrs
487
+ f.utrs.each_slice(2).each { |utr|
488
+ all_sub_blocks << utr
489
+ next if utr.nil?
490
+ utrs << [to_px(utr[0] - @scale_start), to_px((utr[1] - @scale_start) - (utr[0] - @scale_start))]
491
+ }
492
+ end
493
+ f.utrs = utrs
494
+ # If there are any intron like gaps.. get where they would be.
495
+ if not all_sub_blocks.empty?
496
+ all_sub_blocks = all_sub_blocks.sort { |a, b| a.first <=> b.first }
497
+ all_sub_blocks.each_index do |i|
498
+ next if i + 1 == all_sub_blocks.length or all_sub_blocks[i].last >= all_sub_blocks[i + 1].first #skip if there is no gap
499
+ f.block_gaps << [to_px(all_sub_blocks[i].last - @scale_start), to_px((all_sub_blocks[i + 1].first - @scale_start) - (all_sub_blocks[i].last - @scale_start))]
500
+ end
501
+ end
502
+ width = to_px((f.end - @scale_start) - (f.start - @scale_start))
503
+ if track.min_width and width < track.min_width
504
+ width = track.min_width
505
+ end
506
+ y = @track_top + (track.feature_rows[index] * 2 * track.feature_height)
507
+ self.send("draw_#{track.glyph}", {:x => x,
508
+ :y => y,
509
+ :width => width,
510
+ :strand => f.strand,
511
+ :exons => f.exons,
512
+ :utrs => f.utrs,
513
+ :block_gaps => f.block_gaps,
514
+ :height => track.feature_height,
515
+ :params => f.params
516
+ }.merge!(track.args))
517
+ if f.id
518
+ draw_label(:y => y, :x => x+width+2, :text => f.id)
519
+ end
520
+ end
521
+ @track_top += (track.feature_height * track.number_rows * 2) + 20
522
+ end
523
+ @height = @track_top + 100 # Fudge.
524
+ @svg.update_height(@height)
525
+ # @svg.update_width(@width + 200)
526
+ end
527
+
528
+ # ========================================================================= #
529
+ # === to_px
530
+ #
531
+ # Calculates the pixel value for a given number.
532
+ # ========================================================================= #
533
+ def to_px(num)
534
+ (num.to_f / @nt_per_px_x.to_f)
535
+ end
536
+
537
+ # ========================================================================= #
538
+ # === get_markup
539
+ #
540
+ # Generates the SVG text.
541
+ # ========================================================================= #
542
+ def get_markup
543
+ get_limits
544
+ draw_scale
545
+ @tracks.each { |track|
546
+ draw_features(track)
547
+ }
548
+ @svg.draw
549
+ end
550
+
551
+ # ========================================================================= #
552
+ # === draw
553
+ #
554
+ # Prints the svg text to STDOUT. You can capture this and redirect it
555
+ # into a file.
556
+ # ========================================================================= #
557
+ def draw
558
+ puts get_markup
559
+ end
560
+
561
+ # ========================================================================= #
562
+ # === write (write tag)
563
+ #
564
+ # Writes the SVG text to a file
565
+ # ========================================================================= #
566
+ def write(file)
567
+ File.open(file, 'w').write(get_markup)
568
+ end
569
+
570
+ end; end; end