bioroebe 0.10.80
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- checksums.yaml +7 -0
- data/LICENSE.md +428 -0
- data/README.md +9280 -0
- data/bin/advanced_dotplot +7 -0
- data/bin/align_open_reading_frames +12 -0
- data/bin/all_positions_of_this_nucleotide +7 -0
- data/bin/aminoacid_composition +7 -0
- data/bin/aminoacid_frequencies +12 -0
- data/bin/aminoacid_substitution +7 -0
- data/bin/automatically_rename_this_fasta_file +7 -0
- data/bin/base_composition +7 -0
- data/bin/batch_create_windows_executables +7 -0
- data/bin/biomart_console +11 -0
- data/bin/bioroebe +27 -0
- data/bin/bioroebe_controller +10 -0
- data/bin/bioshell +26 -0
- data/bin/blosum_2D_table +12 -0
- data/bin/calculate_n50_value +12 -0
- data/bin/chunked_display +12 -0
- data/bin/codon_frequency +9 -0
- data/bin/codon_to_aminoacid +30 -0
- data/bin/colourize_this_fasta_sequence +14 -0
- data/bin/compact_fasta_file +7 -0
- data/bin/complement +7 -0
- data/bin/complementary_dna_strand +12 -0
- data/bin/complementary_rna_strand +12 -0
- data/bin/compseq +7 -0
- data/bin/consensus_sequence +17 -0
- data/bin/count_AT +12 -0
- data/bin/count_GC +12 -0
- data/bin/create_random_aminoacids +7 -0
- data/bin/decode_this_aminoacid_sequence +20 -0
- data/bin/deduce_aminoacid_sequence +13 -0
- data/bin/deduce_most_likely_aminoacid_sequence +7 -0
- data/bin/display_aminoacid_table +12 -0
- data/bin/display_open_reading_frames +7 -0
- data/bin/dna_to_aminoacid_sequence +7 -0
- data/bin/dna_to_rna +7 -0
- data/bin/downcase_chunked_display +12 -0
- data/bin/download_this_pdb +7 -0
- data/bin/fasta_index +7 -0
- data/bin/fetch_data_from_uniprot +12 -0
- data/bin/filter_away_invalid_nucleotides +12 -0
- data/bin/find_substring +19 -0
- data/bin/genbank_to_fasta +7 -0
- data/bin/hamming_distance +12 -0
- data/bin/input_as_dna +12 -0
- data/bin/is_palindrome +13 -0
- data/bin/leading_five_prime +7 -0
- data/bin/levensthein +7 -0
- data/bin/longest_ORF +14 -0
- data/bin/longest_substring +12 -0
- data/bin/n_stop_codons_in_this_sequence +15 -0
- data/bin/open_reading_frames +14 -0
- data/bin/overwrite_fasta_header +7 -0
- data/bin/palindrome_2D_structure +7 -0
- data/bin/palindrome_generator +7 -0
- data/bin/parse_fasta +7 -0
- data/bin/partner_nucleotide +9 -0
- data/bin/possible_codons_for_this_aminoacid +12 -0
- data/bin/random_dna_sequence +12 -0
- data/bin/random_sequence +12 -0
- data/bin/raw_hamming_distance +12 -0
- data/bin/return_longest_substring_via_LCS_algorithm +7 -0
- data/bin/reverse_complement +7 -0
- data/bin/reverse_sequence +7 -0
- data/bin/ruler +12 -0
- data/bin/scan_this_input_for_startcodons +12 -0
- data/bin/short_aminoacid_letter_from_long_aminoacid_name +7 -0
- data/bin/show_atomic_composition +7 -0
- data/bin/show_codon_usage +12 -0
- data/bin/show_fasta_header +7 -0
- data/bin/show_nucleotide_sequence +7 -0
- data/bin/show_this_codon_table +7 -0
- data/bin/show_this_dna_sequence +7 -0
- data/bin/showorf +14 -0
- data/bin/simplify_fasta +7 -0
- data/bin/sort_aminoacid_based_on_its_hydrophobicity +7 -0
- data/bin/split_this_fasta_file_into_chromosomes +7 -0
- data/bin/strict_filter_away_invalid_aminoacids +7 -0
- data/bin/taxonomy +63 -0
- data/bin/three_to_one +7 -0
- data/bin/to_rna +7 -0
- data/bin/trailing_three_prime +7 -0
- data/bin/trypsin_digest +7 -0
- data/bin/upcase_this_aminoacid_sequence_and_remove_numbers +7 -0
- data/bioroebe.gemspec +97 -0
- data/doc/IUPAC_aminoacids_code.md +36 -0
- data/doc/IUPAC_nucleotide_code.md +19 -0
- data/doc/README.gen +9237 -0
- data/doc/blosum.md +5 -0
- data/doc/compatibility/BIO_PHP.md +37 -0
- data/doc/compatibility/README.md +3 -0
- data/doc/compatibility/emboss.md +56 -0
- data/doc/extensive_usage_example.md +35 -0
- data/doc/german_names_for_the_aminoacids.md +27 -0
- data/doc/instructions_for_the_taxonomy_subproject.md +504 -0
- data/doc/legacy_paths.md +9 -0
- data/doc/pdb_ATOM_entry.md +33 -0
- data/doc/quality_control/README.md +2 -0
- data/doc/quality_control/commandline_applications.md +13 -0
- data/doc/resources.md +23 -0
- data/doc/setup.rb +1655 -0
- data/doc/statistics/statistics.md +41 -0
- data/doc/todo/README.md +5 -0
- data/doc/todo/bioroebe_GUI_todo.md +15 -0
- data/doc/todo/bioroebe_todo.md +2823 -0
- data/doc/using_biomart.md +258 -0
- data/html/test.html +144 -0
- data/lib/bioroebe/aminoacids/aminoacid_substitution.rb +118 -0
- data/lib/bioroebe/aminoacids/aminoacids_mass_table.rb +118 -0
- data/lib/bioroebe/aminoacids/codon_percentage.rb +189 -0
- data/lib/bioroebe/aminoacids/colourize_hydrophilic_and_hydrophobic_aminoacids.rb +110 -0
- data/lib/bioroebe/aminoacids/create_random_aminoacids.rb +221 -0
- data/lib/bioroebe/aminoacids/deduce_aminoacid_sequence.rb +460 -0
- data/lib/bioroebe/aminoacids/display_aminoacid_table.rb +231 -0
- data/lib/bioroebe/aminoacids/show_hydrophobicity.rb +184 -0
- data/lib/bioroebe/annotations/README.md +2 -0
- data/lib/bioroebe/annotations/create_annotation_format.rb +208 -0
- data/lib/bioroebe/autoinclude.rb +7 -0
- data/lib/bioroebe/base/base.rb +35 -0
- data/lib/bioroebe/base/colours.rb +14 -0
- data/lib/bioroebe/base/colours_for_base/colours_for_base.rb +275 -0
- data/lib/bioroebe/base/commandline_application/README.md +7 -0
- data/lib/bioroebe/base/commandline_application/aminoacids.rb +33 -0
- data/lib/bioroebe/base/commandline_application/commandline_application.rb +37 -0
- data/lib/bioroebe/base/commandline_application/commandline_arguments.rb +144 -0
- data/lib/bioroebe/base/commandline_application/directory.rb +33 -0
- data/lib/bioroebe/base/commandline_application/extract.rb +22 -0
- data/lib/bioroebe/base/commandline_application/misc.rb +485 -0
- data/lib/bioroebe/base/commandline_application/opn.rb +47 -0
- data/lib/bioroebe/base/commandline_application/reset.rb +40 -0
- data/lib/bioroebe/base/commandline_application/warnings.rb +36 -0
- data/lib/bioroebe/base/commandline_application/write_what_into.rb +29 -0
- data/lib/bioroebe/base/initialize.rb +18 -0
- data/lib/bioroebe/base/misc.rb +94 -0
- data/lib/bioroebe/base/namespace.rb +16 -0
- data/lib/bioroebe/base/prototype/README.md +12 -0
- data/lib/bioroebe/base/prototype/e_and_ee.rb +24 -0
- data/lib/bioroebe/base/prototype/misc.rb +108 -0
- data/lib/bioroebe/base/prototype/mkdir.rb +20 -0
- data/lib/bioroebe/base/prototype/prototype.rb +21 -0
- data/lib/bioroebe/base/prototype/reset.rb +26 -0
- data/lib/bioroebe/base/reset.rb +11 -0
- data/lib/bioroebe/biomart/LICENSE.md +27 -0
- data/lib/bioroebe/biomart/attribute.rb +77 -0
- data/lib/bioroebe/biomart/biomart.rb +227 -0
- data/lib/bioroebe/biomart/database.rb +128 -0
- data/lib/bioroebe/biomart/dataset.rb +572 -0
- data/lib/bioroebe/biomart/filter.rb +97 -0
- data/lib/bioroebe/biomart/server.rb +152 -0
- data/lib/bioroebe/blosum/blosum.rb +88 -0
- data/lib/bioroebe/calculate/calculate_blosum_score.rb +145 -0
- data/lib/bioroebe/calculate/calculate_gc_content.rb +301 -0
- data/lib/bioroebe/calculate/calculate_levensthein_distance.rb +100 -0
- data/lib/bioroebe/calculate/calculate_melting_temperature.rb +398 -0
- data/lib/bioroebe/calculate/calculate_melting_temperature_for_more_than_thirteen_nucleotides.rb +304 -0
- data/lib/bioroebe/calculate/calculate_the_position_specific_scoring_matrix.rb +166 -0
- data/lib/bioroebe/cell/README.md +1 -0
- data/lib/bioroebe/cell/cell.rb +63 -0
- data/lib/bioroebe/cleave_and_digest/README.md +2 -0
- data/lib/bioroebe/cleave_and_digest/cleave.rb +80 -0
- data/lib/bioroebe/cleave_and_digest/digestion.rb +75 -0
- data/lib/bioroebe/cleave_and_digest/trypsin.rb +192 -0
- data/lib/bioroebe/codon_tables/README.md +9 -0
- data/lib/bioroebe/codon_tables/frequencies/287_Pseudomonas_aeruginosa.yml +101 -0
- data/lib/bioroebe/codon_tables/frequencies/3702_Arabidopsis_thaliana.yml +77 -0
- data/lib/bioroebe/codon_tables/frequencies/4932_Saccharomyces_cerevisiae.yml +103 -0
- data/lib/bioroebe/codon_tables/frequencies/7227_Drosophila_melanogaster.yml +71 -0
- data/lib/bioroebe/codon_tables/frequencies/83333_Escherichia_coli_K12.yml +103 -0
- data/lib/bioroebe/codon_tables/frequencies/9606_Homo_sapiens.yml +123 -0
- data/lib/bioroebe/codon_tables/frequencies/9685_Felis_catus.yml +78 -0
- data/lib/bioroebe/codon_tables/frequencies/README.md +10 -0
- data/lib/bioroebe/codon_tables/frequencies/parse_frequency_table.rb +337 -0
- data/lib/bioroebe/codons/README.md +28 -0
- data/lib/bioroebe/codons/codon_table.rb +416 -0
- data/lib/bioroebe/codons/codon_tables.rb +123 -0
- data/lib/bioroebe/codons/codons.rb +517 -0
- data/lib/bioroebe/codons/convert_this_codon_to_that_aminoacid.rb +102 -0
- data/lib/bioroebe/codons/detect_minimal_codon.rb +180 -0
- data/lib/bioroebe/codons/determine_optimal_codons.rb +74 -0
- data/lib/bioroebe/codons/possible_codons_for_this_aminoacid.rb +380 -0
- data/lib/bioroebe/codons/sanitize_codon_frequency.rb +144 -0
- data/lib/bioroebe/codons/show_codon_tables.rb +130 -0
- data/lib/bioroebe/codons/show_codon_usage.rb +197 -0
- data/lib/bioroebe/codons/show_this_codon_table.rb +573 -0
- data/lib/bioroebe/codons/start_codons.rb +105 -0
- data/lib/bioroebe/colours/colour_schemes/README.md +10 -0
- data/lib/bioroebe/colours/colour_schemes/array_available_colour_schemes.rb +38 -0
- data/lib/bioroebe/colours/colour_schemes/buried.rb +70 -0
- data/lib/bioroebe/colours/colour_schemes/colour_scheme.rb +101 -0
- data/lib/bioroebe/colours/colour_schemes/colour_scheme_demo.rb +262 -0
- data/lib/bioroebe/colours/colour_schemes/helix.rb +65 -0
- data/lib/bioroebe/colours/colour_schemes/hydropathy.rb +70 -0
- data/lib/bioroebe/colours/colour_schemes/nucleotide.rb +47 -0
- data/lib/bioroebe/colours/colour_schemes/score.rb +112 -0
- data/lib/bioroebe/colours/colour_schemes/simple.rb +42 -0
- data/lib/bioroebe/colours/colour_schemes/strand.rb +65 -0
- data/lib/bioroebe/colours/colour_schemes/taylor.rb +58 -0
- data/lib/bioroebe/colours/colour_schemes/turn.rb +65 -0
- data/lib/bioroebe/colours/colour_schemes/zappo.rb +59 -0
- data/lib/bioroebe/colours/colourize_sequence.rb +262 -0
- data/lib/bioroebe/colours/colours.rb +119 -0
- data/lib/bioroebe/colours/misc_colours.rb +80 -0
- data/lib/bioroebe/colours/rev.rb +41 -0
- data/lib/bioroebe/colours/sdir.rb +21 -0
- data/lib/bioroebe/colours/sfancy.rb +21 -0
- data/lib/bioroebe/colours/sfile.rb +21 -0
- data/lib/bioroebe/colours/simp.rb +21 -0
- data/lib/bioroebe/colours/swarn.rb +29 -0
- data/lib/bioroebe/colours/use_colours.rb +27 -0
- data/lib/bioroebe/configuration/configuration.rb +114 -0
- data/lib/bioroebe/configuration/constants.rb +35 -0
- data/lib/bioroebe/constants/GUIs.rb +79 -0
- data/lib/bioroebe/constants/aminoacids_and_proteins.rb +146 -0
- data/lib/bioroebe/constants/base_directory.rb +120 -0
- data/lib/bioroebe/constants/carriage_return.rb +14 -0
- data/lib/bioroebe/constants/codon_tables.rb +77 -0
- data/lib/bioroebe/constants/database_constants.rb +107 -0
- data/lib/bioroebe/constants/files_and_directories.rb +579 -0
- data/lib/bioroebe/constants/misc.rb +189 -0
- data/lib/bioroebe/constants/newline.rb +14 -0
- data/lib/bioroebe/constants/nucleotides.rb +114 -0
- data/lib/bioroebe/constants/regex.rb +28 -0
- data/lib/bioroebe/constants/roebe.rb +38 -0
- data/lib/bioroebe/constants/row_terminator.rb +16 -0
- data/lib/bioroebe/constants/tabulator.rb +14 -0
- data/lib/bioroebe/constants/unicode.rb +12 -0
- data/lib/bioroebe/constants/urls.rb +50 -0
- data/lib/bioroebe/conversions/README.md +3 -0
- data/lib/bioroebe/conversions/convert_aminoacid_to_dna.rb +298 -0
- data/lib/bioroebe/conversions/dna_to_aminoacid_sequence.rb +569 -0
- data/lib/bioroebe/count/README.md +1 -0
- data/lib/bioroebe/count/count_amount_of_aminoacids.rb +352 -0
- data/lib/bioroebe/count/count_amount_of_nucleotides.rb +491 -0
- data/lib/bioroebe/count/count_at.rb +39 -0
- data/lib/bioroebe/count/count_gc.rb +43 -0
- data/lib/bioroebe/css/README.md +5 -0
- data/lib/bioroebe/css/project.css +121 -0
- data/lib/bioroebe/data/README.md +10 -0
- data/lib/bioroebe/data/bam/README.md +1 -0
- data/lib/bioroebe/data/data.txt +192 -0
- data/lib/bioroebe/data/fasta/GFP_mutant_3_coding_sequence.fasta +12 -0
- data/lib/bioroebe/data/fasta/alu_elements.fasta +42 -0
- data/lib/bioroebe/data/fasta/lady_slippers_orchid.fasta +1197 -0
- data/lib/bioroebe/data/fasta/loxP.fasta +2 -0
- data/lib/bioroebe/data/fasta/ls_orchid.fasta +1197 -0
- data/lib/bioroebe/data/fasta/pax6_in_mouse.fasta +1 -0
- data/lib/bioroebe/data/fasta/test.fasta +7 -0
- data/lib/bioroebe/data/fasta/test_DNA.fasta +1 -0
- data/lib/bioroebe/data/fastq/fastq_example_file.fastq +32 -0
- data/lib/bioroebe/data/fastq/fastq_example_file_SP1.fastq +1000 -0
- data/lib/bioroebe/data/fastq/one_random_fastq_entry.fastq +4 -0
- data/lib/bioroebe/data/genbank/sample_file.genbank +15 -0
- data/lib/bioroebe/data/genbank/standard.fasta +3 -0
- data/lib/bioroebe/data/gff/Escherichia_coli_K12_plasmid_F_DNA_NC_002483.1.gff3 +345 -0
- data/lib/bioroebe/data/gff/sample.gff +2 -0
- data/lib/bioroebe/data/gff/test_gene.gff +4 -0
- data/lib/bioroebe/data/gff/transcripts.gff +16 -0
- data/lib/bioroebe/data/gtf/README.md +1 -0
- data/lib/bioroebe/data/json/example_config.json +48 -0
- data/lib/bioroebe/data/pdb/1VII.pdb +754 -0
- data/lib/bioroebe/data/pdb/ala_phe_ala.pdb +228 -0
- data/lib/bioroebe/data/pdb/rcsb_pdb_1VII.fasta +2 -0
- data/lib/bioroebe/data/phylip/README.md +11 -0
- data/lib/bioroebe/data/phylip/example.phylip +7 -0
- data/lib/bioroebe/data/svg/example.svg +301 -0
- data/lib/bioroebe/databases/README.md +1 -0
- data/lib/bioroebe/databases/download_taxonomy_database.rb +102 -0
- data/lib/bioroebe/dotplots/README.md +5 -0
- data/lib/bioroebe/dotplots/advanced_dotplot.rb +256 -0
- data/lib/bioroebe/dotplots/dotplot.rb +184 -0
- data/lib/bioroebe/electron_microscopy/coordinate_analyzer.rb +191 -0
- data/lib/bioroebe/electron_microscopy/fix_pos_file.rb +102 -0
- data/lib/bioroebe/electron_microscopy/generate_em2em_file.rb +122 -0
- data/lib/bioroebe/electron_microscopy/parse_coordinates.rb +197 -0
- data/lib/bioroebe/electron_microscopy/read_file_xmd.rb +282 -0
- data/lib/bioroebe/electron_microscopy/simple_star_file_generator.rb +131 -0
- data/lib/bioroebe/encoding/README.md +2 -0
- data/lib/bioroebe/encoding/encoding.rb +45 -0
- data/lib/bioroebe/enzymes/README.md +2 -0
- data/lib/bioroebe/enzymes/has_this_restriction_enzyme.rb +46 -0
- data/lib/bioroebe/enzymes/restriction_enzyme.rb +200 -0
- data/lib/bioroebe/enzymes/restriction_enzymes_file.rb +72 -0
- data/lib/bioroebe/enzymes/return_restriction_enzyme_sequence_and_cut_position.rb +80 -0
- data/lib/bioroebe/enzymes/return_sequence_that_is_cut_via_restriction_enzyme.rb +65 -0
- data/lib/bioroebe/enzymes/show_restriction_enzymes.rb +119 -0
- data/lib/bioroebe/exceptions/README.md +2 -0
- data/lib/bioroebe/exceptions/exceptions.rb +17 -0
- data/lib/bioroebe/ext/LICENCE.md +5 -0
- data/lib/bioroebe/ext/README.md +7 -0
- data/lib/bioroebe/ext/main.cpp +45 -0
- data/lib/bioroebe/ext/nucleotide.cpp +24 -0
- data/lib/bioroebe/ext/nussinov_algorithm.cpp +348 -0
- data/lib/bioroebe/ext/sequence +0 -0
- data/lib/bioroebe/ext/sequence.cpp +162 -0
- data/lib/bioroebe/fasta_and_fastq/README.md +6 -0
- data/lib/bioroebe/fasta_and_fastq/autocorrect_the_name_of_this_fasta_file.rb +88 -0
- data/lib/bioroebe/fasta_and_fastq/compact_fasta_file/compact_fasta_file.rb +151 -0
- data/lib/bioroebe/fasta_and_fastq/display_how_many_fasta_entries_are_in_this_directory.rb +111 -0
- data/lib/bioroebe/fasta_and_fastq/download_fasta.rb +248 -0
- data/lib/bioroebe/fasta_and_fastq/fasta_defline/README.md +2 -0
- data/lib/bioroebe/fasta_and_fastq/fasta_defline/fasta_defline.rb +113 -0
- data/lib/bioroebe/fasta_and_fastq/fasta_parser.rb +167 -0
- data/lib/bioroebe/fasta_and_fastq/fastq_format_explainer.rb +131 -0
- data/lib/bioroebe/fasta_and_fastq/length_modifier/length_modifier.rb +87 -0
- data/lib/bioroebe/fasta_and_fastq/parse_fasta/constants.rb +50 -0
- data/lib/bioroebe/fasta_and_fastq/parse_fasta/initialize.rb +86 -0
- data/lib/bioroebe/fasta_and_fastq/parse_fasta/menu.rb +117 -0
- data/lib/bioroebe/fasta_and_fastq/parse_fasta/misc.rb +981 -0
- data/lib/bioroebe/fasta_and_fastq/parse_fasta/parse_fasta.rb +27 -0
- data/lib/bioroebe/fasta_and_fastq/parse_fasta/report.rb +156 -0
- data/lib/bioroebe/fasta_and_fastq/parse_fasta/reset.rb +128 -0
- data/lib/bioroebe/fasta_and_fastq/parse_fasta/run.rb +20 -0
- data/lib/bioroebe/fasta_and_fastq/parse_fastq/parse_fastq.rb +83 -0
- data/lib/bioroebe/fasta_and_fastq/return_fasta_subsection_of_this_file.rb +112 -0
- data/lib/bioroebe/fasta_and_fastq/show_fasta_headers.rb +135 -0
- data/lib/bioroebe/fasta_and_fastq/show_fasta_statistics.rb +188 -0
- data/lib/bioroebe/fasta_and_fastq/simplify_fasta_header/simplify_fasta_header.rb +111 -0
- data/lib/bioroebe/fasta_and_fastq/split_this_fasta_file_into_chromosomes/constants.rb +26 -0
- data/lib/bioroebe/fasta_and_fastq/split_this_fasta_file_into_chromosomes/menu.rb +41 -0
- data/lib/bioroebe/fasta_and_fastq/split_this_fasta_file_into_chromosomes/misc.rb +23 -0
- data/lib/bioroebe/fasta_and_fastq/split_this_fasta_file_into_chromosomes/reset.rb +68 -0
- data/lib/bioroebe/fasta_and_fastq/split_this_fasta_file_into_chromosomes/split_this_fasta_file_into_chromosomes.rb +290 -0
- data/lib/bioroebe/genbank/README.md +1 -0
- data/lib/bioroebe/genbank/genbank_flat_file_format_generator.rb +275 -0
- data/lib/bioroebe/genbank/genbank_parser.rb +291 -0
- data/lib/bioroebe/gene/gene.rb +64 -0
- data/lib/bioroebe/genomes/genome_pattern.rb +165 -0
- data/lib/bioroebe/genomes/genome_retriever.rb +79 -0
- data/lib/bioroebe/gui/experimental/README.md +1 -0
- data/lib/bioroebe/gui/experimental/snapgene/snapgene.rb +147 -0
- data/lib/bioroebe/gui/gtk3/README.md +2 -0
- data/lib/bioroebe/gui/gtk3/alignment/alignment.rb +337 -0
- data/lib/bioroebe/gui/gtk3/aminoacid_composition/aminoacid_composition.rb +510 -0
- data/lib/bioroebe/gui/gtk3/aminoacid_composition/customized_dialog.rb +55 -0
- data/lib/bioroebe/gui/gtk3/anti_sense_strand/anti_sense_strand.config +6 -0
- data/lib/bioroebe/gui/gtk3/anti_sense_strand/anti_sense_strand.rb +29 -0
- data/lib/bioroebe/gui/gtk3/blosum_matrix_viewer/blosum_matrix_viewer.rb +196 -0
- data/lib/bioroebe/gui/gtk3/calculate_cell_numbers_of_bacteria/calculate_cell_numbers_of_bacteria.config +6 -0
- data/lib/bioroebe/gui/gtk3/calculate_cell_numbers_of_bacteria/calculate_cell_numbers_of_bacteria.rb +106 -0
- data/lib/bioroebe/gui/gtk3/controller/controller.rb +406 -0
- data/lib/bioroebe/gui/gtk3/dna_to_aminoacid_widget/dna_to_aminoacid_widget.rb +609 -0
- data/lib/bioroebe/gui/gtk3/dna_to_reverse_complement_widget/dna_to_reverse_complement_widget.config +6 -0
- data/lib/bioroebe/gui/gtk3/dna_to_reverse_complement_widget/dna_to_reverse_complement_widget.rb +189 -0
- data/lib/bioroebe/gui/gtk3/fasta_table_widget/fasta_table_widget.rb +245 -0
- data/lib/bioroebe/gui/gtk3/format_converter/format_converter.rb +346 -0
- data/lib/bioroebe/gui/gtk3/gene/gene.rb +182 -0
- data/lib/bioroebe/gui/gtk3/hamming_distance/hamming_distance.config +6 -0
- data/lib/bioroebe/gui/gtk3/hamming_distance/hamming_distance.rb +370 -0
- data/lib/bioroebe/gui/gtk3/levensthein_distance/levensthein_distance.config +6 -0
- data/lib/bioroebe/gui/gtk3/levensthein_distance/levensthein_distance.rb +175 -0
- data/lib/bioroebe/gui/gtk3/nucleotide_analyser/nucleotide_analyser.rb +428 -0
- data/lib/bioroebe/gui/gtk3/parse_pdb_file/parse_pdb_file.rb +342 -0
- data/lib/bioroebe/gui/gtk3/primer_design_widget/primer_design_widget.rb +580 -0
- data/lib/bioroebe/gui/gtk3/protein_to_DNA/protein_to_DNA.config +6 -0
- data/lib/bioroebe/gui/gtk3/protein_to_DNA/protein_to_DNA.rb +182 -0
- data/lib/bioroebe/gui/gtk3/random_sequence/random_sequence.rb +566 -0
- data/lib/bioroebe/gui/gtk3/restriction_enzymes/restriction_enzymes.config +6 -0
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- data/lib/bioroebe/gui/gtk3/show_codon_table/misc.rb +556 -0
- data/lib/bioroebe/gui/gtk3/show_codon_table/show_codon_table.rb +171 -0
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- data/lib/bioroebe/gui/libui/calculate_cell_numbers_of_bacteria/calculate_cell_numbers_of_bacteria.rb +60 -0
- data/lib/bioroebe/gui/libui/dna_to_aminoacid_widget/dna_to_aminoacid_widget.rb +161 -0
- data/lib/bioroebe/gui/libui/dna_to_reverse_complement_widget/dna_to_reverse_complement_widget.rb +76 -0
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- data/lib/bioroebe/gui/shared_code/calculate_cell_numbers_of_bacteria/calculate_cell_numbers_of_bacteria_module.rb +216 -0
- data/lib/bioroebe/gui/shared_code/dna_to_reverse_complement_widget/dna_to_reverse_complement_widget_module.rb +284 -0
- data/lib/bioroebe/gui/shared_code/levensthein_distance/levensthein_distance_module.rb +402 -0
- data/lib/bioroebe/gui/shared_code/protein_to_DNA/protein_to_DNA_module.rb +192 -0
- data/lib/bioroebe/gui/shared_code/show_codon_table/show_codon_table_module.rb +72 -0
- data/lib/bioroebe/gui/shared_code/show_codon_usage/show_codon_usage_module.rb +213 -0
- data/lib/bioroebe/gui/tk/aminoacid_composition/aminoacid_composition.rb +206 -0
- data/lib/bioroebe/gui/tk/blosum_matrix_viewer/blosum_matrix_viewer.rb +140 -0
- data/lib/bioroebe/gui/tk/hamming_distance/hamming_distance.rb +262 -0
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- data/lib/bioroebe/gui/tk/three_to_one/three_to_one.rb +199 -0
- data/lib/bioroebe/gui/unified_widgets/anti_sense_strand/anti_sense_strand.rb +519 -0
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- data/lib/bioroebe/nucleotides/show_nucleotide_sequence.rb +556 -0
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- data/lib/bioroebe/palindromes/palindrome_finder.rb +208 -0
- data/lib/bioroebe/palindromes/palindrome_generator.rb +272 -0
- data/lib/bioroebe/parsers/biolang_parser.rb +156 -0
- data/lib/bioroebe/parsers/blosum_parser.rb +222 -0
- data/lib/bioroebe/parsers/genbank_parser.rb +78 -0
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- data/lib/bioroebe/patterns/analyse_glycosylation_pattern.rb +149 -0
- data/lib/bioroebe/patterns/is_this_sequence_a_EGF2_pattern.rb +66 -0
- data/lib/bioroebe/patterns/profile_pattern.rb +182 -0
- data/lib/bioroebe/patterns/rgg_scanner.rb +160 -0
- data/lib/bioroebe/patterns/scan_for_repeat.rb +157 -0
- data/lib/bioroebe/pdb/download_this_pdb.rb +67 -0
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- data/lib/bioroebe/pdb/report_secondary_structures_from_this_pdb_file.rb +225 -0
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- data/lib/bioroebe/project/project.rb +264 -0
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- data/lib/bioroebe/regexes/README.md +2 -0
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- data/lib/bioroebe/toplevel_methods/calculate_n50_value.rb +57 -0
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- data/lib/bioroebe/toplevel_methods/complement.rb +58 -0
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- data/lib/bioroebe/toplevel_methods/leading_five_prime_and_trailing_three_prime.rb +101 -0
- data/lib/bioroebe/toplevel_methods/levensthein.rb +63 -0
- data/lib/bioroebe/toplevel_methods/log_directory.rb +109 -0
- data/lib/bioroebe/toplevel_methods/longest_common_substring.rb +55 -0
- data/lib/bioroebe/toplevel_methods/map_ncbi_entry_to_eutils_id.rb +88 -0
- data/lib/bioroebe/toplevel_methods/matches.rb +259 -0
- data/lib/bioroebe/toplevel_methods/misc.rb +432 -0
- data/lib/bioroebe/toplevel_methods/nucleotides.rb +715 -0
- data/lib/bioroebe/toplevel_methods/number_of_clones.rb +63 -0
- data/lib/bioroebe/toplevel_methods/open_in_browser.rb +77 -0
- data/lib/bioroebe/toplevel_methods/open_reading_frames.rb +236 -0
- data/lib/bioroebe/toplevel_methods/opn.rb +34 -0
- data/lib/bioroebe/toplevel_methods/palindromes.rb +127 -0
- data/lib/bioroebe/toplevel_methods/parse.rb +59 -0
- data/lib/bioroebe/toplevel_methods/phred_error_probability.rb +68 -0
- data/lib/bioroebe/toplevel_methods/rds.rb +24 -0
- data/lib/bioroebe/toplevel_methods/remove.rb +86 -0
- data/lib/bioroebe/toplevel_methods/return_source_code_of_this_method.rb +35 -0
- data/lib/bioroebe/toplevel_methods/return_subsequence_based_on_indices.rb +68 -0
- data/lib/bioroebe/toplevel_methods/rna_splicing.rb +73 -0
- data/lib/bioroebe/toplevel_methods/rnalfold.rb +69 -0
- data/lib/bioroebe/toplevel_methods/searching_and_finding.rb +116 -0
- data/lib/bioroebe/toplevel_methods/shuffleseq.rb +37 -0
- data/lib/bioroebe/toplevel_methods/statistics.rb +53 -0
- data/lib/bioroebe/toplevel_methods/sum_of_odd_integers.rb +62 -0
- data/lib/bioroebe/toplevel_methods/taxonomy.rb +187 -0
- data/lib/bioroebe/toplevel_methods/three_delimiter.rb +34 -0
- data/lib/bioroebe/toplevel_methods/time_and_date.rb +53 -0
- data/lib/bioroebe/toplevel_methods/to_camelcase.rb +26 -0
- data/lib/bioroebe/toplevel_methods/truncate.rb +48 -0
- data/lib/bioroebe/toplevel_methods/url.rb +36 -0
- data/lib/bioroebe/toplevel_methods/verbose.rb +59 -0
- data/lib/bioroebe/utility_scripts/align_open_reading_frames.rb +191 -0
- data/lib/bioroebe/utility_scripts/analyse_local_dataset.rb +119 -0
- data/lib/bioroebe/utility_scripts/check_for_mismatches/check_for_mismatches.rb +230 -0
- data/lib/bioroebe/utility_scripts/compacter.rb +131 -0
- data/lib/bioroebe/utility_scripts/compseq/compseq.rb +529 -0
- data/lib/bioroebe/utility_scripts/consensus_sequence.rb +374 -0
- data/lib/bioroebe/utility_scripts/create_batch_entrez_file.rb +130 -0
- data/lib/bioroebe/utility_scripts/determine_antigenic_areas.rb +115 -0
- data/lib/bioroebe/utility_scripts/determine_missing_nucleotides_percentage.rb +137 -0
- data/lib/bioroebe/utility_scripts/display_open_reading_frames/determine.rb +73 -0
- data/lib/bioroebe/utility_scripts/display_open_reading_frames/display_open_reading_frames.rb +31 -0
- data/lib/bioroebe/utility_scripts/display_open_reading_frames/initialize.rb +37 -0
- data/lib/bioroebe/utility_scripts/display_open_reading_frames/menu.rb +49 -0
- data/lib/bioroebe/utility_scripts/display_open_reading_frames/misc.rb +471 -0
- data/lib/bioroebe/utility_scripts/display_open_reading_frames/report.rb +113 -0
- data/lib/bioroebe/utility_scripts/display_open_reading_frames/reset.rb +56 -0
- data/lib/bioroebe/utility_scripts/dot_alignment.rb +177 -0
- data/lib/bioroebe/utility_scripts/download_files_from_rebase.rb +72 -0
- data/lib/bioroebe/utility_scripts/find_gene.rb +202 -0
- data/lib/bioroebe/utility_scripts/mirror_repeat.rb +235 -0
- data/lib/bioroebe/utility_scripts/move_file_to_its_correct_location.rb +151 -0
- data/lib/bioroebe/utility_scripts/parse_taxonomy.rb +168 -0
- data/lib/bioroebe/utility_scripts/pathways.rb +152 -0
- data/lib/bioroebe/utility_scripts/permutations.rb +145 -0
- data/lib/bioroebe/utility_scripts/punnet/punnet.rb +126 -0
- data/lib/bioroebe/utility_scripts/show_this_dna_sequence.rb +148 -0
- data/lib/bioroebe/utility_scripts/showorf/constants.rb +36 -0
- data/lib/bioroebe/utility_scripts/showorf/help.rb +33 -0
- data/lib/bioroebe/utility_scripts/showorf/initialize.rb +52 -0
- data/lib/bioroebe/utility_scripts/showorf/menu.rb +68 -0
- data/lib/bioroebe/utility_scripts/showorf/reset.rb +39 -0
- data/lib/bioroebe/utility_scripts/showorf/run.rb +152 -0
- data/lib/bioroebe/utility_scripts/showorf/show.rb +97 -0
- data/lib/bioroebe/utility_scripts/showorf/showorf.rb +488 -0
- data/lib/bioroebe/version/version.rb +44 -0
- data/lib/bioroebe/viennarna/README.md +3 -0
- data/lib/bioroebe/viennarna/rnafold_wrapper.rb +196 -0
- data/lib/bioroebe/with_gui.rb +18 -0
- data/lib/bioroebe/www/bioroebe.cgi +44 -0
- data/lib/bioroebe/www/embeddable_interface.rb +686 -0
- data/lib/bioroebe/www/sinatra/sinatra.rb +1013 -0
- data/lib/bioroebe/yaml/agarose/agarose_concentrations.yml +21 -0
- data/lib/bioroebe/yaml/aminoacids/amino_acids.yml +92 -0
- data/lib/bioroebe/yaml/aminoacids/amino_acids_abbreviations.yml +31 -0
- data/lib/bioroebe/yaml/aminoacids/amino_acids_average_mass_table.yml +33 -0
- data/lib/bioroebe/yaml/aminoacids/amino_acids_classification.yml +18 -0
- data/lib/bioroebe/yaml/aminoacids/amino_acids_english.yml +26 -0
- data/lib/bioroebe/yaml/aminoacids/amino_acids_frequency.yml +44 -0
- data/lib/bioroebe/yaml/aminoacids/amino_acids_long_name_to_one_letter.yml +61 -0
- data/lib/bioroebe/yaml/aminoacids/amino_acids_molecular_formula.yml +32 -0
- data/lib/bioroebe/yaml/aminoacids/amino_acids_monoisotopic_mass_table.yml +38 -0
- data/lib/bioroebe/yaml/aminoacids/amino_acids_reste.yml +35 -0
- data/lib/bioroebe/yaml/aminoacids/amino_acids_three_to_one.yml +34 -0
- data/lib/bioroebe/yaml/aminoacids/hydropathy_table.yml +44 -0
- data/lib/bioroebe/yaml/aminoacids/molecular_weight.yml +29 -0
- data/lib/bioroebe/yaml/aminoacids/simple_aminoacids.yml +66 -0
- data/lib/bioroebe/yaml/aminoacids/weight_of_common_proteins.yml +33 -0
- data/lib/bioroebe/yaml/antisense/antisense.yml +9 -0
- data/lib/bioroebe/yaml/base_composition_of_dna.yml +37 -0
- data/lib/bioroebe/yaml/blosum/blosum45.yml +36 -0
- data/lib/bioroebe/yaml/blosum/blosum50.yml +34 -0
- data/lib/bioroebe/yaml/blosum/blosum62.yml +35 -0
- data/lib/bioroebe/yaml/blosum/blosum80.yml +37 -0
- data/lib/bioroebe/yaml/blosum/blosum90.yml +36 -0
- data/lib/bioroebe/yaml/blosum/blosum_matrix.yml +200 -0
- data/lib/bioroebe/yaml/chromosomes/chromosome_numbers.yml +30 -0
- data/lib/bioroebe/yaml/codon_tables/1.yml +113 -0
- data/lib/bioroebe/yaml/codon_tables/10.yml +89 -0
- data/lib/bioroebe/yaml/codon_tables/11.yml +91 -0
- data/lib/bioroebe/yaml/codon_tables/12.yml +89 -0
- data/lib/bioroebe/yaml/codon_tables/13.yml +89 -0
- data/lib/bioroebe/yaml/codon_tables/14.yml +89 -0
- data/lib/bioroebe/yaml/codon_tables/15.yml +94 -0
- data/lib/bioroebe/yaml/codon_tables/16.yml +89 -0
- data/lib/bioroebe/yaml/codon_tables/2.yml +86 -0
- data/lib/bioroebe/yaml/codon_tables/21.yml +89 -0
- data/lib/bioroebe/yaml/codon_tables/22.yml +89 -0
- data/lib/bioroebe/yaml/codon_tables/23.yml +91 -0
- data/lib/bioroebe/yaml/codon_tables/24.yml +89 -0
- data/lib/bioroebe/yaml/codon_tables/25.yml +89 -0
- data/lib/bioroebe/yaml/codon_tables/26.yml +96 -0
- data/lib/bioroebe/yaml/codon_tables/27.yml +104 -0
- data/lib/bioroebe/yaml/codon_tables/28.yml +97 -0
- data/lib/bioroebe/yaml/codon_tables/29.yml +93 -0
- data/lib/bioroebe/yaml/codon_tables/3.yml +98 -0
- data/lib/bioroebe/yaml/codon_tables/30.yml +91 -0
- data/lib/bioroebe/yaml/codon_tables/31.yml +94 -0
- data/lib/bioroebe/yaml/codon_tables/33.yml +101 -0
- data/lib/bioroebe/yaml/codon_tables/4.yml +96 -0
- data/lib/bioroebe/yaml/codon_tables/5.yml +100 -0
- data/lib/bioroebe/yaml/codon_tables/6.yml +96 -0
- data/lib/bioroebe/yaml/codon_tables/9.yml +97 -0
- data/lib/bioroebe/yaml/codon_tables/overview.yml +42 -0
- data/lib/bioroebe/yaml/configuration/README.md +12 -0
- data/lib/bioroebe/yaml/configuration/browser.yml +1 -0
- data/lib/bioroebe/yaml/configuration/colourize_fasta_sequences.yml +14 -0
- data/lib/bioroebe/yaml/configuration/default_colours_for_the_aminoacids.yml +28 -0
- data/lib/bioroebe/yaml/configuration/temp_dir.yml +1 -0
- data/lib/bioroebe/yaml/configuration/try_to_use_matplotlib.yml +1 -0
- data/lib/bioroebe/yaml/configuration/use_opn.yml +1 -0
- data/lib/bioroebe/yaml/configuration/use_this_database.yml +1 -0
- data/lib/bioroebe/yaml/create_these_directories_on_startup/create_these_directories_on_startup.yml +9 -0
- data/lib/bioroebe/yaml/default_dna_input.yml +3 -0
- data/lib/bioroebe/yaml/enzymes/enzyme_classes.yml +15 -0
- data/lib/bioroebe/yaml/enzymes/pH-Optima.yml +11 -0
- data/lib/bioroebe/yaml/fasta_and_fastq/fastq_quality_schemes.yml +44 -0
- data/lib/bioroebe/yaml/genomes/README.md +16 -0
- data/lib/bioroebe/yaml/humans/README.md +2 -0
- data/lib/bioroebe/yaml/humans/human_chromosomes.yml +53 -0
- data/lib/bioroebe/yaml/laboratory/README.md +1 -0
- data/lib/bioroebe/yaml/laboratory/pipettes.yml +8 -0
- data/lib/bioroebe/yaml/mRNA/mRNA.yml +16 -0
- data/lib/bioroebe/yaml/nuclear_localization_sequences.yml +15 -0
- data/lib/bioroebe/yaml/nucleotides/abbreviations_for_nucleotides.yml +29 -0
- data/lib/bioroebe/yaml/nucleotides/nucleotide_density.yml +10 -0
- data/lib/bioroebe/yaml/nucleotides/nucleotides.yml +34 -0
- data/lib/bioroebe/yaml/nucleotides/nucleotides_weight.yml +12 -0
- data/lib/bioroebe/yaml/pathways/README.md +2 -0
- data/lib/bioroebe/yaml/pathways/citric_acid_cycle.yml +16 -0
- data/lib/bioroebe/yaml/pathways/glycolysis.yml +20 -0
- data/lib/bioroebe/yaml/pathways/shikimate_pathway.yml +23 -0
- data/lib/bioroebe/yaml/pathways/urea_cycle.yml +11 -0
- data/lib/bioroebe/yaml/primers/README.md +4 -0
- data/lib/bioroebe/yaml/primers/primers.yml +3 -0
- data/lib/bioroebe/yaml/promoters/35S.yml +15 -0
- data/lib/bioroebe/yaml/promoters/strong_promoters.yml +24 -0
- data/lib/bioroebe/yaml/proteases/proteases.yml +3 -0
- data/lib/bioroebe/yaml/proteins/ubiquitin.yml +4 -0
- data/lib/bioroebe/yaml/remote_urls/README.md +4 -0
- data/lib/bioroebe/yaml/remote_urls/remote_urls.yml +3 -0
- data/lib/bioroebe/yaml/restriction_enzymes/restriction_enzymes.yml +630 -0
- data/lib/bioroebe/yaml/sequences/JX472995_Green_fluorescent_protein_from_Aequorea_victoria.fasta +14 -0
- data/lib/bioroebe/yaml/sequences/README.md +2 -0
- data/lib/bioroebe/yaml/talens.yml +22 -0
- data/lib/bioroebe/yaml/viruses/ecoli_phages.yml +63 -0
- data/lib/bioroebe/yaml/viruses/viruses.yml +6 -0
- data/lib/bioroebe.rb +5 -0
- data/spec/testing_toplevel_method_editor.rb +20 -0
- data/spec/testing_toplevel_method_url.rb +15 -0
- data/spec/testing_toplevel_method_verbose.rb +13 -0
- data/test/advanced_svg_example.rb +307 -0
- data/test/testing_bioroebe.rb +25 -0
- data/test/testing_codons.rb +45 -0
- data/test/testing_dna_to_rna_conversion.rb +15 -0
- data/test/testing_parse_pdb_file.rb +23 -0
- data/test/testing_reverse_complement.rb +32 -0
- data/test/testing_svg_component_of_bioroebe.rb +311 -0
- data/test/testing_svg_component_of_bioroebe_from_json_dataset.rb +34 -0
- data/test/testing_taxonomy.rb +22 -0
- metadata +1059 -0
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#!/usr/bin/ruby -w
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# =========================================================================== #
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# === Bioroebe::SVG::MiniFeature
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#
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# operation.
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attr_accessor :id
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attr_accessor :start
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attr_accessor :strand
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attr_accessor :exons
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attr_accessor :utrs
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# :id = the name for the feature such as the gene name or transcript ID
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#
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# )
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#
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reset
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end
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# =========================================================================== #
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module Bioroebe
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#
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# Arguments:
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#
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# height: this is the minimum height of the rendered page.
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# width: this is the minimum width of the rendered page.
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# background_color: this is the background color of the page
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# (default is none), can be any SVG colour
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|
+
# eg rgb(256,0,0) or #FF0000.
|
45
|
+
#
|
46
|
+
# ========================================================================= #
|
47
|
+
def initialize(i)
|
48
|
+
@height = i[:height]
|
49
|
+
@width = i[:width]
|
50
|
+
i[:style] = "background-color:#{i[:background_color]};" if i[:background_color]
|
51
|
+
# ======================================================================= #
|
52
|
+
# === @svg
|
53
|
+
#
|
54
|
+
# Instantiate a new SVGEE object next.
|
55
|
+
# ======================================================================= #
|
56
|
+
@svg = SVGEE.new(i)
|
57
|
+
@num_intervals = i[:number_of_intervals]
|
58
|
+
@svg.update_height(@height)
|
59
|
+
reset
|
60
|
+
end
|
61
|
+
|
62
|
+
# ========================================================================= #
|
63
|
+
# === reset (reset tag)
|
64
|
+
# ========================================================================= #
|
65
|
+
def reset
|
66
|
+
# ======================================================================= #
|
67
|
+
# === @track_top
|
68
|
+
# ======================================================================= #
|
69
|
+
@track_top = 30
|
70
|
+
# ======================================================================= #
|
71
|
+
# === @tracks
|
72
|
+
# ======================================================================= #
|
73
|
+
@tracks = [] # Array of track objects with loads of features in it.
|
74
|
+
@scale_start = 1.0/0.0
|
75
|
+
@scale_stop = -1.0/0.0
|
76
|
+
@nt_per_percent = 1
|
77
|
+
end
|
78
|
+
|
79
|
+
# ========================================================================= #
|
80
|
+
# === Bioroebe::SVG::Page.from_json
|
81
|
+
#
|
82
|
+
# Takes a custom-written json file and uses it to generate an SVG
|
83
|
+
# document based on the information in that page.
|
84
|
+
#
|
85
|
+
#* :json a JSON file describing the parameters and files needed to build
|
86
|
+
# an SVG document
|
87
|
+
# ========================================================================= #
|
88
|
+
def self.from_json(args)
|
89
|
+
require 'json'
|
90
|
+
data = JSON.parse(File.open(args[:json], 'r').read)
|
91
|
+
p = Page.new(:width => data['Page']['width'],
|
92
|
+
:height => data['Page']['height'],
|
93
|
+
:number_of_intervals => data['Page']['intervals'])
|
94
|
+
data['Tracks'].each { |track|
|
95
|
+
# Prepare the tracks-arguments.
|
96
|
+
track_args = track.dup
|
97
|
+
track_args.delete('file')
|
98
|
+
track_args.delete('file_type')
|
99
|
+
track_args = track_args.inject({}) { |memo, (k, v)| memo[k.to_sym] = v; memo }
|
100
|
+
# ===================================================================== #
|
101
|
+
# Convert any of the pre-made gradient strings in the keys
|
102
|
+
# to symbol.
|
103
|
+
# ===================================================================== #
|
104
|
+
track_args.each_key { |k|
|
105
|
+
next unless track_args[k].respond_to?(:to_sym)
|
106
|
+
track_args[k] = track_args[k].to_sym if Glyph.gradients.include?(track_args[k].to_sym)
|
107
|
+
}
|
108
|
+
svg_track = p.add_track(track_args)
|
109
|
+
features = [] ##set up the features...
|
110
|
+
case track['file_type'] # Deal with the gff and data files.
|
111
|
+
# ===================================================================== #
|
112
|
+
# === gff
|
113
|
+
# ===================================================================== #
|
114
|
+
when 'gff'
|
115
|
+
groups = {}
|
116
|
+
parentless_features = []
|
117
|
+
Page.parse_gff(track['file']).each { |gff| #gets features in a list and links their children to them as members of the array at the key
|
118
|
+
parent_id = gff.attributes.select { |a| a.first == 'Parent' }
|
119
|
+
if parent_id.empty?
|
120
|
+
parentless_features << gff
|
121
|
+
else
|
122
|
+
if groups[parent_id.first.last].nil?
|
123
|
+
groups[parent_id.first.last] = []
|
124
|
+
groups[parent_id.first.last] << gff
|
125
|
+
else
|
126
|
+
groups[parent_id.first.last] << gff
|
127
|
+
end
|
128
|
+
end
|
129
|
+
}
|
130
|
+
# =================================================================== #
|
131
|
+
# Now flick through the parentless features and add any
|
132
|
+
# exons or UTRs.
|
133
|
+
# =================================================================== #
|
134
|
+
parentless_features.each { |plf|
|
135
|
+
gff_id = plf.attributes.select { |a| a.first == 'ID' }
|
136
|
+
gff_id = gff_id.first.last
|
137
|
+
exons = []
|
138
|
+
utrs = []
|
139
|
+
children = groups[gff_id] || children = []
|
140
|
+
children.each { |child|
|
141
|
+
case child.feature
|
142
|
+
when 'exon','CDS'
|
143
|
+
exons += [child.start, child.end]
|
144
|
+
when /utr/i # Handle UTRs here.
|
145
|
+
utrs += [child.start, child.end]
|
146
|
+
end
|
147
|
+
}
|
148
|
+
features << MiniFeature.new(
|
149
|
+
:start => plf.start,
|
150
|
+
:end => plf.end,
|
151
|
+
:exons => exons,
|
152
|
+
:utrs => utrs,
|
153
|
+
:strand => plf.strand,
|
154
|
+
:id => gff_id
|
155
|
+
)
|
156
|
+
} # parentless features end
|
157
|
+
# ===================================================================== #
|
158
|
+
# === data
|
159
|
+
# ===================================================================== #
|
160
|
+
when 'data'
|
161
|
+
# =================================================================== #
|
162
|
+
# Each line is a data feature.
|
163
|
+
# =================================================================== #
|
164
|
+
File.open(track['file'], 'r').each { |line|
|
165
|
+
start, stop, value = line.split(/\t/)
|
166
|
+
features << MiniFeature.new(
|
167
|
+
:start => start.to_i,
|
168
|
+
:end => stop.to_i,
|
169
|
+
:segment_height => value.to_f
|
170
|
+
)
|
171
|
+
}
|
172
|
+
end
|
173
|
+
features.each { |f| svg_track.add(f) }
|
174
|
+
} # track end
|
175
|
+
p.write(args[:outfile])
|
176
|
+
end
|
177
|
+
|
178
|
+
# ========================================================================= #
|
179
|
+
# === add_track
|
180
|
+
#
|
181
|
+
# Adds a new Bioroebe::SVG::Track object to the current Bioroebe::SVG::Page
|
182
|
+
# object.
|
183
|
+
#
|
184
|
+
#==args
|
185
|
+
#
|
186
|
+
#* :glyph = one of Bioroebe::SVG::Glyphs#glyphs currently
|
187
|
+
# [:generic, :directed, :transcript, :scale, :label, :histogram, :circle, :down_triangle, :up_triangle, :span]
|
188
|
+
#* :stroke_color = the outline colour of the glyphs in the track (default = "black"), can be any SVG colour eg rgb(256,0,0) or #FF0000
|
189
|
+
#* :fill_color = the fill colour of the glyphs in the track (default = 'red'), can be any SVG colour eg rgb(256,0,0) or #FF0000, or one of the built in gradient types Bioroebe::SVG::Glyph#gradients
|
190
|
+
# [:red_white_h, :green_white_h, :blue_white_h, :yellow_white_h, :red_white_radial, :green_white_radial, :blue_white_radial, :yellow_white_radial ]
|
191
|
+
# or a custom definition of a gradient.
|
192
|
+
#
|
193
|
+
# {:type => :radial,
|
194
|
+
# :id => :custom,
|
195
|
+
# :cx => 5,
|
196
|
+
# :cy => 5,
|
197
|
+
# :r => 50,
|
198
|
+
# :fx => 50,
|
199
|
+
# :fy => 50,
|
200
|
+
# :stops => [ {
|
201
|
+
# :offset => 0,
|
202
|
+
# :color => 'rgb(255,255,255)',
|
203
|
+
# :opacity => 0
|
204
|
+
# }, {
|
205
|
+
# :offset => 100,
|
206
|
+
# :color => 'rgb(0,127,200)',
|
207
|
+
# :opacity => 1
|
208
|
+
# }, ]
|
209
|
+
# }
|
210
|
+
#
|
211
|
+
# * :track_height = minimum height for the track, will be modified automatically if more space is needed e.g for overlapping features (default = auto),
|
212
|
+
# * :name = a displayed name for the track (default = 'feature_track')
|
213
|
+
# * :label = display the name given to the track (default = true),
|
214
|
+
# * :stroke_width = width in pixels of the outline of the glyphs (default=1)
|
215
|
+
# * :x_round = x radius of the ellipse used to round off the corners of rectangles (default = 1)
|
216
|
+
# * :y_round = y radius of the ellipse used to round off the corners of rectangles (default = 1)
|
217
|
+
# :utr_fill_color = the fill colour of the utr part of the glyph (default = 'black'), can be any SVG colour eg rgb(256,0,0) or #FF0000, or one of the built in gradient types Bioroebe::SVG::Glyph#gradients
|
218
|
+
# [:red_white_h, :green_white_h, :blue_white_h, :yellow_white_h, :red_white_radial, :green_white_radial, :blue_white_radial, :yellow_white_radial ]
|
219
|
+
#
|
220
|
+
# or a custom definition of a gradient
|
221
|
+
#
|
222
|
+
# {:type => :radial,
|
223
|
+
# :id => :custom,
|
224
|
+
# :cx => 5,
|
225
|
+
# :cy => 5,
|
226
|
+
# :r => 50,
|
227
|
+
# :fx => 50,
|
228
|
+
# :fy => 50,
|
229
|
+
# :stops => [ {
|
230
|
+
# :offset => 0,
|
231
|
+
# :color => 'rgb(255,255,255)',
|
232
|
+
# :opacity => 0
|
233
|
+
# }, {
|
234
|
+
# :offset => 100,
|
235
|
+
# :color => 'rgb(0,127,200)',
|
236
|
+
# :opacity => 1
|
237
|
+
# }, ]
|
238
|
+
# }
|
239
|
+
#* :utr_stroke = the outline colour of the utr part of the glyph (default = "black"), can be any SVG colour eg rgb(256,0,0) or #FF0000
|
240
|
+
#* :utr_stroke_width = The width of the outline stroke for the utr part of the glyph (default = 1)
|
241
|
+
#* :exon_fill_color = the fill colour of the utr part of the glyph (default = 'red'), can be any SVG colour eg rgb(256,0,0) or #FF0000, or one of the built in gradient types Bioroebe::SVG::Glyph#gradients or a custom definition of a gradient
|
242
|
+
# [:red_white_h, :green_white_h, :blue_white_h, :yellow_white_h, :red_white_radial, :green_white_radial, :blue_white_radial, :yellow_white_radial ]
|
243
|
+
#or a custom definition of a gradient
|
244
|
+
# {:type => :radial,
|
245
|
+
# :id => :custom,
|
246
|
+
# :cx => 5,
|
247
|
+
# :cy => 5,
|
248
|
+
# :r => 50,
|
249
|
+
# :fx => 50,
|
250
|
+
# :fy => 50,
|
251
|
+
# :stops => [ {
|
252
|
+
# :offset => 0,
|
253
|
+
# :color => 'rgb(255,255,255)',
|
254
|
+
# :opacity => 0
|
255
|
+
# }, {
|
256
|
+
# :offset => 100,
|
257
|
+
# :color => 'rgb(0,127,200)',
|
258
|
+
# :opacity => 1
|
259
|
+
# }, ]
|
260
|
+
#
|
261
|
+
# :exon_stroke = the outline colour of the exon part of the glyph
|
262
|
+
# (default = "black") can be any SVG colour eg rgb(256,0,0) or #FF0000
|
263
|
+
# :exon_stroke_width = The width of the outline stroke for the exon part
|
264
|
+
# of the glyph (default = 1)
|
265
|
+
# :line_color = the colour for the line part that joins the blocks (default = 'black') can be any SVG colour eg rgb(256,0,0) or #FF0000
|
266
|
+
# :line_width = the width ffor the line part that joins the blocks (default = 1)
|
267
|
+
# :exon_style = an arbitrary SVG compliant style string eg "fill-opacity:0.4;"
|
268
|
+
# :utr_style = an arbitrary SVG compliant style string eg "fill-opacity:0.4;"
|
269
|
+
# :line_style = an arbitrary SVG compliant style string eg "fill-opacity:0.4;"
|
270
|
+
# :gap_marker = style of the line between blocks - either angled or straight
|
271
|
+
#
|
272
|
+
# Will return:
|
273
|
+
#
|
274
|
+
# a new Bioroebe::SVG::Track object
|
275
|
+
#
|
276
|
+
# ========================================================================= #
|
277
|
+
def add_track(args)
|
278
|
+
# ======================================================================= #
|
279
|
+
# Sort out the colour/gradient options
|
280
|
+
# ======================================================================= #
|
281
|
+
[:fill_color, :exon_fill_color, :utr_fill_color].each { |colour_tag|
|
282
|
+
if Glyph.gradients.include?(args[colour_tag])
|
283
|
+
@svg.gradient(Glyph.gradient(args[colour_tag]))
|
284
|
+
args[colour_tag] = "url(##{args[colour_tag]})"
|
285
|
+
elsif args[colour_tag].instance_of?(Hash)
|
286
|
+
@svg.gradient(args[colour_tag])
|
287
|
+
args[colour_tag] = "url(##{args[colour_tag][:id]})"
|
288
|
+
end
|
289
|
+
}
|
290
|
+
@tracks << Track.new(args)
|
291
|
+
return @tracks.last
|
292
|
+
end
|
293
|
+
|
294
|
+
# ========================================================================= #
|
295
|
+
# === get_limits
|
296
|
+
#
|
297
|
+
# Calculates the Page scale-start and scale-stop position and the
|
298
|
+
# nucleotides per pixel for the current Page.
|
299
|
+
# ========================================================================= #
|
300
|
+
def get_limits
|
301
|
+
@tracks.each { |track|
|
302
|
+
lowest = track.features.sort { |x, y|
|
303
|
+
x.start <=> y.start
|
304
|
+
}.first.start
|
305
|
+
highest = track.features.sort { |x, y| y.end <=> x.end }.first.end
|
306
|
+
@scale_start = lowest if lowest < @scale_start
|
307
|
+
@scale_stop = highest if highest > @scale_stop
|
308
|
+
@nt_per_px_x = (@scale_stop - @scale_start).to_f / @width.to_f
|
309
|
+
}
|
310
|
+
begin
|
311
|
+
old_nt_per_px_x = @nt_per_px_x
|
312
|
+
@tracks.each { |track|
|
313
|
+
highest = track.features.map { |feat|
|
314
|
+
compute_boundaries(feat)[1]
|
315
|
+
}.max
|
316
|
+
if highest > @scale_stop
|
317
|
+
@scale_stop = highest
|
318
|
+
@nt_per_px_x = (@scale_stop - @scale_start).to_f / @width.to_f
|
319
|
+
end
|
320
|
+
}
|
321
|
+
end while (@nt_per_px_x - old_nt_per_px_x).abs > 1
|
322
|
+
end
|
323
|
+
|
324
|
+
# ========================================================================= #
|
325
|
+
# === compute_boundaries
|
326
|
+
# ========================================================================= #
|
327
|
+
def compute_boundaries(feature)
|
328
|
+
feat_end = feature.end
|
329
|
+
feat_end += (8 * @nt_per_px_x * feature.id.to_s.length).to_i if feature.id and @nt_per_px_x
|
330
|
+
[feature.start, feat_end]
|
331
|
+
end
|
332
|
+
|
333
|
+
# ========================================================================= #
|
334
|
+
# === draw_scale
|
335
|
+
#
|
336
|
+
# Adds scale bar to the list of objects to be rendered in the final.
|
337
|
+
# ========================================================================= #
|
338
|
+
def draw_scale
|
339
|
+
Glyph.scale(:start => @scale_start,
|
340
|
+
:stop => @scale_stop,
|
341
|
+
:number_of_intervals => @num_intervals,
|
342
|
+
:page_width => @width).each { |entry| @svg.add_primitive(entry) }
|
343
|
+
end
|
344
|
+
|
345
|
+
# ========================================================================= #
|
346
|
+
# === Page.parse_gff
|
347
|
+
#
|
348
|
+
# This method will parse a GFF file into an Array of
|
349
|
+
# Bio::GFF::GFF3::Record[http://bioruby.org/rdoc/Bio/GFF/GFF3/Record.html] objects
|
350
|
+
#* gff_file - a GFF-formatted file
|
351
|
+
#* returns - an Array of Bio::GFF::GFF3::Record[http://bioruby.org/rdoc/Bio/GFF/GFF3/Record.html] objects
|
352
|
+
# ========================================================================= #
|
353
|
+
def self.parse_gff(
|
354
|
+
gff_file = HOME_DIRECTORY_OF_USER_X+'DATA/PROGRAMMING_LANGUAGES/RUBY/src/bioroebe/test/test_gene.gff'
|
355
|
+
)
|
356
|
+
require 'bio' # This depends on 'bio' gem for now, unortunately.
|
357
|
+
a = []
|
358
|
+
File.open(gff_file).each { |line|
|
359
|
+
next if line =~ /^#/
|
360
|
+
a << Bio::GFF::GFF3::Record.new(line)
|
361
|
+
}
|
362
|
+
a
|
363
|
+
end
|
364
|
+
|
365
|
+
# ========================================================================= #
|
366
|
+
# === draw_label
|
367
|
+
#
|
368
|
+
# Adds the Bioroebe::SVG::Primitive objects to the SVGEE object
|
369
|
+
#* +args+ - an Array of Bioroebe::SVG::Primitive object
|
370
|
+
# ========================================================================= #
|
371
|
+
def draw_label(args)
|
372
|
+
Glyph.label(:text => args[:text],
|
373
|
+
:x => args[:x],
|
374
|
+
:y => args[:y]).each { |g| @svg.add_primitive(g) }
|
375
|
+
end
|
376
|
+
|
377
|
+
# ========================================================================= #
|
378
|
+
# === draw_generic
|
379
|
+
# ========================================================================= #
|
380
|
+
def draw_generic(args) #remember presentation info comes from track@args when the track is defined
|
381
|
+
Glyph.generic(args).each { |g| @svg.add_primitive(g) }
|
382
|
+
end
|
383
|
+
|
384
|
+
# ========================================================================= #
|
385
|
+
# === draw_directed
|
386
|
+
# ========================================================================= #
|
387
|
+
def draw_directed(args)
|
388
|
+
Glyph.directed(args).each { |g| @svg.add_primitive(g) }
|
389
|
+
end
|
390
|
+
|
391
|
+
# ========================================================================= #
|
392
|
+
# === draw_circle
|
393
|
+
# ========================================================================= #
|
394
|
+
def draw_circle(args)
|
395
|
+
Glyph.circle(args).each { |g| @svg.add_primitive(g) }
|
396
|
+
end
|
397
|
+
|
398
|
+
# ========================================================================= #
|
399
|
+
# === draw_transcript
|
400
|
+
# ========================================================================= #
|
401
|
+
def draw_transcript(args)
|
402
|
+
Glyph.transcript(args).each { |g| @svg.add_primitive(g) }
|
403
|
+
end
|
404
|
+
|
405
|
+
# ========================================================================= #
|
406
|
+
# === draw_histogram
|
407
|
+
# ========================================================================= #
|
408
|
+
def draw_histogram(args)
|
409
|
+
Glyph.generic(args).each { |g| @svg.add_primitive(g) }
|
410
|
+
end
|
411
|
+
|
412
|
+
# ========================================================================= #
|
413
|
+
# === draw_up_triangle
|
414
|
+
# ========================================================================= #
|
415
|
+
def draw_up_triangle(args)
|
416
|
+
Glyph.up_triangle(args).each { |g| @svg.add_primitive(g) }
|
417
|
+
end
|
418
|
+
|
419
|
+
# ========================================================================= #
|
420
|
+
# === draw_down_triangle
|
421
|
+
# ========================================================================= #
|
422
|
+
def draw_down_triangle(args)
|
423
|
+
Glyph.down_triangle(args).each { |g| @svg.add_primitive(g) }
|
424
|
+
end
|
425
|
+
|
426
|
+
# ========================================================================= #
|
427
|
+
# === draw_span
|
428
|
+
# ========================================================================= #
|
429
|
+
def draw_span(args)
|
430
|
+
Glyph.span(args).each { |g|
|
431
|
+
@svg.add_primitive(g)
|
432
|
+
}
|
433
|
+
end
|
434
|
+
|
435
|
+
# ========================================================================= #
|
436
|
+
# === draw_features
|
437
|
+
#
|
438
|
+
# Takes a Bioroebe::SVG::Track object and does the work to draw features.
|
439
|
+
#
|
440
|
+
# It examines the the type of track and calculates the required
|
441
|
+
# parameters * +args+ - an Array of Bioroebe::SVG::Track object
|
442
|
+
# ========================================================================= #
|
443
|
+
def draw_features(track)
|
444
|
+
if [:histogram, 'histogram'].include?(track.glyph) #do different stuff for data tracks...
|
445
|
+
y = @track_top + (track.track_height)
|
446
|
+
max = track.max_y ? track.max_y : track.features.sort { |a, b| a.segment_height <=> b.segment_height }.last.segment_height
|
447
|
+
min = 0
|
448
|
+
if track.label
|
449
|
+
draw_label(:text => track.name, :y => @track_top += 30, :x => 3)
|
450
|
+
end
|
451
|
+
draw_label(:text => max, :x => to_px(@scale_stop - @scale_start) + 5, :y => @track_top + 5)
|
452
|
+
draw_label(:text => min, :x => to_px(@scale_stop - @scale_start) + 5, :y => @track_top + track.track_height + 5)
|
453
|
+
data_interval = max - min
|
454
|
+
data_per_px = track.track_height.to_f / data_interval.to_f
|
455
|
+
track.features.each do |f|
|
456
|
+
x = to_px(f.start - @scale_start)
|
457
|
+
width = to_px((f.end - @scale_start) - (f.start - @scale_start))
|
458
|
+
height = f.segment_height.to_f * data_per_px
|
459
|
+
y = @track_top + track.track_height - height + 5
|
460
|
+
#$stderr.puts f.segment_height, data_per_px, data_interval, max, min, "------"
|
461
|
+
self.send("draw_#{track.glyph}", {:x => x,
|
462
|
+
:y => y,
|
463
|
+
:width => width,
|
464
|
+
:height => height}.merge!(track.args))
|
465
|
+
end
|
466
|
+
@track_top += (track.track_height) + 20
|
467
|
+
else ##following stuff for the features
|
468
|
+
if track.label
|
469
|
+
draw_label(:text => track.name, :y => @track_top += 30, :x => 3)
|
470
|
+
end
|
471
|
+
track.get_rows(self) ##work out how many rows and which features belong in each row...
|
472
|
+
track.features.each_with_index do |f, index|
|
473
|
+
x = to_px(f.start - @scale_start) #bottom left of feature
|
474
|
+
all_sub_blocks = []
|
475
|
+
#convert the exon and utr start stops to px start stops and px widths
|
476
|
+
exons = []
|
477
|
+
if f.exons
|
478
|
+
f.exons.each_slice(2).each { |exon|
|
479
|
+
all_sub_blocks << exon
|
480
|
+
next if exon.nil?
|
481
|
+
exons << [to_px(exon[0] - @scale_start), to_px((exon[1] - @scale_start) - (exon[0] - @scale_start))]
|
482
|
+
}
|
483
|
+
end
|
484
|
+
f.exons = exons
|
485
|
+
utrs = []
|
486
|
+
if f.utrs
|
487
|
+
f.utrs.each_slice(2).each { |utr|
|
488
|
+
all_sub_blocks << utr
|
489
|
+
next if utr.nil?
|
490
|
+
utrs << [to_px(utr[0] - @scale_start), to_px((utr[1] - @scale_start) - (utr[0] - @scale_start))]
|
491
|
+
}
|
492
|
+
end
|
493
|
+
f.utrs = utrs
|
494
|
+
# If there are any intron like gaps.. get where they would be.
|
495
|
+
if not all_sub_blocks.empty?
|
496
|
+
all_sub_blocks = all_sub_blocks.sort { |a, b| a.first <=> b.first }
|
497
|
+
all_sub_blocks.each_index do |i|
|
498
|
+
next if i + 1 == all_sub_blocks.length or all_sub_blocks[i].last >= all_sub_blocks[i + 1].first #skip if there is no gap
|
499
|
+
f.block_gaps << [to_px(all_sub_blocks[i].last - @scale_start), to_px((all_sub_blocks[i + 1].first - @scale_start) - (all_sub_blocks[i].last - @scale_start))]
|
500
|
+
end
|
501
|
+
end
|
502
|
+
width = to_px((f.end - @scale_start) - (f.start - @scale_start))
|
503
|
+
if track.min_width and width < track.min_width
|
504
|
+
width = track.min_width
|
505
|
+
end
|
506
|
+
y = @track_top + (track.feature_rows[index] * 2 * track.feature_height)
|
507
|
+
self.send("draw_#{track.glyph}", {:x => x,
|
508
|
+
:y => y,
|
509
|
+
:width => width,
|
510
|
+
:strand => f.strand,
|
511
|
+
:exons => f.exons,
|
512
|
+
:utrs => f.utrs,
|
513
|
+
:block_gaps => f.block_gaps,
|
514
|
+
:height => track.feature_height,
|
515
|
+
:params => f.params
|
516
|
+
}.merge!(track.args))
|
517
|
+
if f.id
|
518
|
+
draw_label(:y => y, :x => x+width+2, :text => f.id)
|
519
|
+
end
|
520
|
+
end
|
521
|
+
@track_top += (track.feature_height * track.number_rows * 2) + 20
|
522
|
+
end
|
523
|
+
@height = @track_top + 100 # Fudge.
|
524
|
+
@svg.update_height(@height)
|
525
|
+
# @svg.update_width(@width + 200)
|
526
|
+
end
|
527
|
+
|
528
|
+
# ========================================================================= #
|
529
|
+
# === to_px
|
530
|
+
#
|
531
|
+
# Calculates the pixel value for a given number.
|
532
|
+
# ========================================================================= #
|
533
|
+
def to_px(num)
|
534
|
+
(num.to_f / @nt_per_px_x.to_f)
|
535
|
+
end
|
536
|
+
|
537
|
+
# ========================================================================= #
|
538
|
+
# === get_markup
|
539
|
+
#
|
540
|
+
# Generates the SVG text.
|
541
|
+
# ========================================================================= #
|
542
|
+
def get_markup
|
543
|
+
get_limits
|
544
|
+
draw_scale
|
545
|
+
@tracks.each { |track|
|
546
|
+
draw_features(track)
|
547
|
+
}
|
548
|
+
@svg.draw
|
549
|
+
end
|
550
|
+
|
551
|
+
# ========================================================================= #
|
552
|
+
# === draw
|
553
|
+
#
|
554
|
+
# Prints the svg text to STDOUT. You can capture this and redirect it
|
555
|
+
# into a file.
|
556
|
+
# ========================================================================= #
|
557
|
+
def draw
|
558
|
+
puts get_markup
|
559
|
+
end
|
560
|
+
|
561
|
+
# ========================================================================= #
|
562
|
+
# === write (write tag)
|
563
|
+
#
|
564
|
+
# Writes the SVG text to a file
|
565
|
+
# ========================================================================= #
|
566
|
+
def write(file)
|
567
|
+
File.open(file, 'w').write(get_markup)
|
568
|
+
end
|
569
|
+
|
570
|
+
end; end; end
|