bioroebe 0.10.80
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- checksums.yaml +7 -0
- data/LICENSE.md +428 -0
- data/README.md +9280 -0
- data/bin/advanced_dotplot +7 -0
- data/bin/align_open_reading_frames +12 -0
- data/bin/all_positions_of_this_nucleotide +7 -0
- data/bin/aminoacid_composition +7 -0
- data/bin/aminoacid_frequencies +12 -0
- data/bin/aminoacid_substitution +7 -0
- data/bin/automatically_rename_this_fasta_file +7 -0
- data/bin/base_composition +7 -0
- data/bin/batch_create_windows_executables +7 -0
- data/bin/biomart_console +11 -0
- data/bin/bioroebe +27 -0
- data/bin/bioroebe_controller +10 -0
- data/bin/bioshell +26 -0
- data/bin/blosum_2D_table +12 -0
- data/bin/calculate_n50_value +12 -0
- data/bin/chunked_display +12 -0
- data/bin/codon_frequency +9 -0
- data/bin/codon_to_aminoacid +30 -0
- data/bin/colourize_this_fasta_sequence +14 -0
- data/bin/compact_fasta_file +7 -0
- data/bin/complement +7 -0
- data/bin/complementary_dna_strand +12 -0
- data/bin/complementary_rna_strand +12 -0
- data/bin/compseq +7 -0
- data/bin/consensus_sequence +17 -0
- data/bin/count_AT +12 -0
- data/bin/count_GC +12 -0
- data/bin/create_random_aminoacids +7 -0
- data/bin/decode_this_aminoacid_sequence +20 -0
- data/bin/deduce_aminoacid_sequence +13 -0
- data/bin/deduce_most_likely_aminoacid_sequence +7 -0
- data/bin/display_aminoacid_table +12 -0
- data/bin/display_open_reading_frames +7 -0
- data/bin/dna_to_aminoacid_sequence +7 -0
- data/bin/dna_to_rna +7 -0
- data/bin/downcase_chunked_display +12 -0
- data/bin/download_this_pdb +7 -0
- data/bin/fasta_index +7 -0
- data/bin/fetch_data_from_uniprot +12 -0
- data/bin/filter_away_invalid_nucleotides +12 -0
- data/bin/find_substring +19 -0
- data/bin/genbank_to_fasta +7 -0
- data/bin/hamming_distance +12 -0
- data/bin/input_as_dna +12 -0
- data/bin/is_palindrome +13 -0
- data/bin/leading_five_prime +7 -0
- data/bin/levensthein +7 -0
- data/bin/longest_ORF +14 -0
- data/bin/longest_substring +12 -0
- data/bin/n_stop_codons_in_this_sequence +15 -0
- data/bin/open_reading_frames +14 -0
- data/bin/overwrite_fasta_header +7 -0
- data/bin/palindrome_2D_structure +7 -0
- data/bin/palindrome_generator +7 -0
- data/bin/parse_fasta +7 -0
- data/bin/partner_nucleotide +9 -0
- data/bin/possible_codons_for_this_aminoacid +12 -0
- data/bin/random_dna_sequence +12 -0
- data/bin/random_sequence +12 -0
- data/bin/raw_hamming_distance +12 -0
- data/bin/return_longest_substring_via_LCS_algorithm +7 -0
- data/bin/reverse_complement +7 -0
- data/bin/reverse_sequence +7 -0
- data/bin/ruler +12 -0
- data/bin/scan_this_input_for_startcodons +12 -0
- data/bin/short_aminoacid_letter_from_long_aminoacid_name +7 -0
- data/bin/show_atomic_composition +7 -0
- data/bin/show_codon_usage +12 -0
- data/bin/show_fasta_header +7 -0
- data/bin/show_nucleotide_sequence +7 -0
- data/bin/show_this_codon_table +7 -0
- data/bin/show_this_dna_sequence +7 -0
- data/bin/showorf +14 -0
- data/bin/simplify_fasta +7 -0
- data/bin/sort_aminoacid_based_on_its_hydrophobicity +7 -0
- data/bin/split_this_fasta_file_into_chromosomes +7 -0
- data/bin/strict_filter_away_invalid_aminoacids +7 -0
- data/bin/taxonomy +63 -0
- data/bin/three_to_one +7 -0
- data/bin/to_rna +7 -0
- data/bin/trailing_three_prime +7 -0
- data/bin/trypsin_digest +7 -0
- data/bin/upcase_this_aminoacid_sequence_and_remove_numbers +7 -0
- data/bioroebe.gemspec +97 -0
- data/doc/IUPAC_aminoacids_code.md +36 -0
- data/doc/IUPAC_nucleotide_code.md +19 -0
- data/doc/README.gen +9237 -0
- data/doc/blosum.md +5 -0
- data/doc/compatibility/BIO_PHP.md +37 -0
- data/doc/compatibility/README.md +3 -0
- data/doc/compatibility/emboss.md +56 -0
- data/doc/extensive_usage_example.md +35 -0
- data/doc/german_names_for_the_aminoacids.md +27 -0
- data/doc/instructions_for_the_taxonomy_subproject.md +504 -0
- data/doc/legacy_paths.md +9 -0
- data/doc/pdb_ATOM_entry.md +33 -0
- data/doc/quality_control/README.md +2 -0
- data/doc/quality_control/commandline_applications.md +13 -0
- data/doc/resources.md +23 -0
- data/doc/setup.rb +1655 -0
- data/doc/statistics/statistics.md +41 -0
- data/doc/todo/README.md +5 -0
- data/doc/todo/bioroebe_GUI_todo.md +15 -0
- data/doc/todo/bioroebe_todo.md +2823 -0
- data/doc/using_biomart.md +258 -0
- data/html/test.html +144 -0
- data/lib/bioroebe/aminoacids/aminoacid_substitution.rb +118 -0
- data/lib/bioroebe/aminoacids/aminoacids_mass_table.rb +118 -0
- data/lib/bioroebe/aminoacids/codon_percentage.rb +189 -0
- data/lib/bioroebe/aminoacids/colourize_hydrophilic_and_hydrophobic_aminoacids.rb +110 -0
- data/lib/bioroebe/aminoacids/create_random_aminoacids.rb +221 -0
- data/lib/bioroebe/aminoacids/deduce_aminoacid_sequence.rb +460 -0
- data/lib/bioroebe/aminoacids/display_aminoacid_table.rb +231 -0
- data/lib/bioroebe/aminoacids/show_hydrophobicity.rb +184 -0
- data/lib/bioroebe/annotations/README.md +2 -0
- data/lib/bioroebe/annotations/create_annotation_format.rb +208 -0
- data/lib/bioroebe/autoinclude.rb +7 -0
- data/lib/bioroebe/base/base.rb +35 -0
- data/lib/bioroebe/base/colours.rb +14 -0
- data/lib/bioroebe/base/colours_for_base/colours_for_base.rb +275 -0
- data/lib/bioroebe/base/commandline_application/README.md +7 -0
- data/lib/bioroebe/base/commandline_application/aminoacids.rb +33 -0
- data/lib/bioroebe/base/commandline_application/commandline_application.rb +37 -0
- data/lib/bioroebe/base/commandline_application/commandline_arguments.rb +144 -0
- data/lib/bioroebe/base/commandline_application/directory.rb +33 -0
- data/lib/bioroebe/base/commandline_application/extract.rb +22 -0
- data/lib/bioroebe/base/commandline_application/misc.rb +485 -0
- data/lib/bioroebe/base/commandline_application/opn.rb +47 -0
- data/lib/bioroebe/base/commandline_application/reset.rb +40 -0
- data/lib/bioroebe/base/commandline_application/warnings.rb +36 -0
- data/lib/bioroebe/base/commandline_application/write_what_into.rb +29 -0
- data/lib/bioroebe/base/initialize.rb +18 -0
- data/lib/bioroebe/base/misc.rb +94 -0
- data/lib/bioroebe/base/namespace.rb +16 -0
- data/lib/bioroebe/base/prototype/README.md +12 -0
- data/lib/bioroebe/base/prototype/e_and_ee.rb +24 -0
- data/lib/bioroebe/base/prototype/misc.rb +108 -0
- data/lib/bioroebe/base/prototype/mkdir.rb +20 -0
- data/lib/bioroebe/base/prototype/prototype.rb +21 -0
- data/lib/bioroebe/base/prototype/reset.rb +26 -0
- data/lib/bioroebe/base/reset.rb +11 -0
- data/lib/bioroebe/biomart/LICENSE.md +27 -0
- data/lib/bioroebe/biomart/attribute.rb +77 -0
- data/lib/bioroebe/biomart/biomart.rb +227 -0
- data/lib/bioroebe/biomart/database.rb +128 -0
- data/lib/bioroebe/biomart/dataset.rb +572 -0
- data/lib/bioroebe/biomart/filter.rb +97 -0
- data/lib/bioroebe/biomart/server.rb +152 -0
- data/lib/bioroebe/blosum/blosum.rb +88 -0
- data/lib/bioroebe/calculate/calculate_blosum_score.rb +145 -0
- data/lib/bioroebe/calculate/calculate_gc_content.rb +301 -0
- data/lib/bioroebe/calculate/calculate_levensthein_distance.rb +100 -0
- data/lib/bioroebe/calculate/calculate_melting_temperature.rb +398 -0
- data/lib/bioroebe/calculate/calculate_melting_temperature_for_more_than_thirteen_nucleotides.rb +304 -0
- data/lib/bioroebe/calculate/calculate_the_position_specific_scoring_matrix.rb +166 -0
- data/lib/bioroebe/cell/README.md +1 -0
- data/lib/bioroebe/cell/cell.rb +63 -0
- data/lib/bioroebe/cleave_and_digest/README.md +2 -0
- data/lib/bioroebe/cleave_and_digest/cleave.rb +80 -0
- data/lib/bioroebe/cleave_and_digest/digestion.rb +75 -0
- data/lib/bioroebe/cleave_and_digest/trypsin.rb +192 -0
- data/lib/bioroebe/codon_tables/README.md +9 -0
- data/lib/bioroebe/codon_tables/frequencies/287_Pseudomonas_aeruginosa.yml +101 -0
- data/lib/bioroebe/codon_tables/frequencies/3702_Arabidopsis_thaliana.yml +77 -0
- data/lib/bioroebe/codon_tables/frequencies/4932_Saccharomyces_cerevisiae.yml +103 -0
- data/lib/bioroebe/codon_tables/frequencies/7227_Drosophila_melanogaster.yml +71 -0
- data/lib/bioroebe/codon_tables/frequencies/83333_Escherichia_coli_K12.yml +103 -0
- data/lib/bioroebe/codon_tables/frequencies/9606_Homo_sapiens.yml +123 -0
- data/lib/bioroebe/codon_tables/frequencies/9685_Felis_catus.yml +78 -0
- data/lib/bioroebe/codon_tables/frequencies/README.md +10 -0
- data/lib/bioroebe/codon_tables/frequencies/parse_frequency_table.rb +337 -0
- data/lib/bioroebe/codons/README.md +28 -0
- data/lib/bioroebe/codons/codon_table.rb +416 -0
- data/lib/bioroebe/codons/codon_tables.rb +123 -0
- data/lib/bioroebe/codons/codons.rb +517 -0
- data/lib/bioroebe/codons/convert_this_codon_to_that_aminoacid.rb +102 -0
- data/lib/bioroebe/codons/detect_minimal_codon.rb +180 -0
- data/lib/bioroebe/codons/determine_optimal_codons.rb +74 -0
- data/lib/bioroebe/codons/possible_codons_for_this_aminoacid.rb +380 -0
- data/lib/bioroebe/codons/sanitize_codon_frequency.rb +144 -0
- data/lib/bioroebe/codons/show_codon_tables.rb +130 -0
- data/lib/bioroebe/codons/show_codon_usage.rb +197 -0
- data/lib/bioroebe/codons/show_this_codon_table.rb +573 -0
- data/lib/bioroebe/codons/start_codons.rb +105 -0
- data/lib/bioroebe/colours/colour_schemes/README.md +10 -0
- data/lib/bioroebe/colours/colour_schemes/array_available_colour_schemes.rb +38 -0
- data/lib/bioroebe/colours/colour_schemes/buried.rb +70 -0
- data/lib/bioroebe/colours/colour_schemes/colour_scheme.rb +101 -0
- data/lib/bioroebe/colours/colour_schemes/colour_scheme_demo.rb +262 -0
- data/lib/bioroebe/colours/colour_schemes/helix.rb +65 -0
- data/lib/bioroebe/colours/colour_schemes/hydropathy.rb +70 -0
- data/lib/bioroebe/colours/colour_schemes/nucleotide.rb +47 -0
- data/lib/bioroebe/colours/colour_schemes/score.rb +112 -0
- data/lib/bioroebe/colours/colour_schemes/simple.rb +42 -0
- data/lib/bioroebe/colours/colour_schemes/strand.rb +65 -0
- data/lib/bioroebe/colours/colour_schemes/taylor.rb +58 -0
- data/lib/bioroebe/colours/colour_schemes/turn.rb +65 -0
- data/lib/bioroebe/colours/colour_schemes/zappo.rb +59 -0
- data/lib/bioroebe/colours/colourize_sequence.rb +262 -0
- data/lib/bioroebe/colours/colours.rb +119 -0
- data/lib/bioroebe/colours/misc_colours.rb +80 -0
- data/lib/bioroebe/colours/rev.rb +41 -0
- data/lib/bioroebe/colours/sdir.rb +21 -0
- data/lib/bioroebe/colours/sfancy.rb +21 -0
- data/lib/bioroebe/colours/sfile.rb +21 -0
- data/lib/bioroebe/colours/simp.rb +21 -0
- data/lib/bioroebe/colours/swarn.rb +29 -0
- data/lib/bioroebe/colours/use_colours.rb +27 -0
- data/lib/bioroebe/configuration/configuration.rb +114 -0
- data/lib/bioroebe/configuration/constants.rb +35 -0
- data/lib/bioroebe/constants/GUIs.rb +79 -0
- data/lib/bioroebe/constants/aminoacids_and_proteins.rb +146 -0
- data/lib/bioroebe/constants/base_directory.rb +120 -0
- data/lib/bioroebe/constants/carriage_return.rb +14 -0
- data/lib/bioroebe/constants/codon_tables.rb +77 -0
- data/lib/bioroebe/constants/database_constants.rb +107 -0
- data/lib/bioroebe/constants/files_and_directories.rb +579 -0
- data/lib/bioroebe/constants/misc.rb +189 -0
- data/lib/bioroebe/constants/newline.rb +14 -0
- data/lib/bioroebe/constants/nucleotides.rb +114 -0
- data/lib/bioroebe/constants/regex.rb +28 -0
- data/lib/bioroebe/constants/roebe.rb +38 -0
- data/lib/bioroebe/constants/row_terminator.rb +16 -0
- data/lib/bioroebe/constants/tabulator.rb +14 -0
- data/lib/bioroebe/constants/unicode.rb +12 -0
- data/lib/bioroebe/constants/urls.rb +50 -0
- data/lib/bioroebe/conversions/README.md +3 -0
- data/lib/bioroebe/conversions/convert_aminoacid_to_dna.rb +298 -0
- data/lib/bioroebe/conversions/dna_to_aminoacid_sequence.rb +569 -0
- data/lib/bioroebe/count/README.md +1 -0
- data/lib/bioroebe/count/count_amount_of_aminoacids.rb +352 -0
- data/lib/bioroebe/count/count_amount_of_nucleotides.rb +491 -0
- data/lib/bioroebe/count/count_at.rb +39 -0
- data/lib/bioroebe/count/count_gc.rb +43 -0
- data/lib/bioroebe/css/README.md +5 -0
- data/lib/bioroebe/css/project.css +121 -0
- data/lib/bioroebe/data/README.md +10 -0
- data/lib/bioroebe/data/bam/README.md +1 -0
- data/lib/bioroebe/data/data.txt +192 -0
- data/lib/bioroebe/data/fasta/GFP_mutant_3_coding_sequence.fasta +12 -0
- data/lib/bioroebe/data/fasta/alu_elements.fasta +42 -0
- data/lib/bioroebe/data/fasta/lady_slippers_orchid.fasta +1197 -0
- data/lib/bioroebe/data/fasta/loxP.fasta +2 -0
- data/lib/bioroebe/data/fasta/ls_orchid.fasta +1197 -0
- data/lib/bioroebe/data/fasta/pax6_in_mouse.fasta +1 -0
- data/lib/bioroebe/data/fasta/test.fasta +7 -0
- data/lib/bioroebe/data/fasta/test_DNA.fasta +1 -0
- data/lib/bioroebe/data/fastq/fastq_example_file.fastq +32 -0
- data/lib/bioroebe/data/fastq/fastq_example_file_SP1.fastq +1000 -0
- data/lib/bioroebe/data/fastq/one_random_fastq_entry.fastq +4 -0
- data/lib/bioroebe/data/genbank/sample_file.genbank +15 -0
- data/lib/bioroebe/data/genbank/standard.fasta +3 -0
- data/lib/bioroebe/data/gff/Escherichia_coli_K12_plasmid_F_DNA_NC_002483.1.gff3 +345 -0
- data/lib/bioroebe/data/gff/sample.gff +2 -0
- data/lib/bioroebe/data/gff/test_gene.gff +4 -0
- data/lib/bioroebe/data/gff/transcripts.gff +16 -0
- data/lib/bioroebe/data/gtf/README.md +1 -0
- data/lib/bioroebe/data/json/example_config.json +48 -0
- data/lib/bioroebe/data/pdb/1VII.pdb +754 -0
- data/lib/bioroebe/data/pdb/ala_phe_ala.pdb +228 -0
- data/lib/bioroebe/data/pdb/rcsb_pdb_1VII.fasta +2 -0
- data/lib/bioroebe/data/phylip/README.md +11 -0
- data/lib/bioroebe/data/phylip/example.phylip +7 -0
- data/lib/bioroebe/data/svg/example.svg +301 -0
- data/lib/bioroebe/databases/README.md +1 -0
- data/lib/bioroebe/databases/download_taxonomy_database.rb +102 -0
- data/lib/bioroebe/dotplots/README.md +5 -0
- data/lib/bioroebe/dotplots/advanced_dotplot.rb +256 -0
- data/lib/bioroebe/dotplots/dotplot.rb +184 -0
- data/lib/bioroebe/electron_microscopy/coordinate_analyzer.rb +191 -0
- data/lib/bioroebe/electron_microscopy/fix_pos_file.rb +102 -0
- data/lib/bioroebe/electron_microscopy/generate_em2em_file.rb +122 -0
- data/lib/bioroebe/electron_microscopy/parse_coordinates.rb +197 -0
- data/lib/bioroebe/electron_microscopy/read_file_xmd.rb +282 -0
- data/lib/bioroebe/electron_microscopy/simple_star_file_generator.rb +131 -0
- data/lib/bioroebe/encoding/README.md +2 -0
- data/lib/bioroebe/encoding/encoding.rb +45 -0
- data/lib/bioroebe/enzymes/README.md +2 -0
- data/lib/bioroebe/enzymes/has_this_restriction_enzyme.rb +46 -0
- data/lib/bioroebe/enzymes/restriction_enzyme.rb +200 -0
- data/lib/bioroebe/enzymes/restriction_enzymes_file.rb +72 -0
- data/lib/bioroebe/enzymes/return_restriction_enzyme_sequence_and_cut_position.rb +80 -0
- data/lib/bioroebe/enzymes/return_sequence_that_is_cut_via_restriction_enzyme.rb +65 -0
- data/lib/bioroebe/enzymes/show_restriction_enzymes.rb +119 -0
- data/lib/bioroebe/exceptions/README.md +2 -0
- data/lib/bioroebe/exceptions/exceptions.rb +17 -0
- data/lib/bioroebe/ext/LICENCE.md +5 -0
- data/lib/bioroebe/ext/README.md +7 -0
- data/lib/bioroebe/ext/main.cpp +45 -0
- data/lib/bioroebe/ext/nucleotide.cpp +24 -0
- data/lib/bioroebe/ext/nussinov_algorithm.cpp +348 -0
- data/lib/bioroebe/ext/sequence +0 -0
- data/lib/bioroebe/ext/sequence.cpp +162 -0
- data/lib/bioroebe/fasta_and_fastq/README.md +6 -0
- data/lib/bioroebe/fasta_and_fastq/autocorrect_the_name_of_this_fasta_file.rb +88 -0
- data/lib/bioroebe/fasta_and_fastq/compact_fasta_file/compact_fasta_file.rb +151 -0
- data/lib/bioroebe/fasta_and_fastq/display_how_many_fasta_entries_are_in_this_directory.rb +111 -0
- data/lib/bioroebe/fasta_and_fastq/download_fasta.rb +248 -0
- data/lib/bioroebe/fasta_and_fastq/fasta_defline/README.md +2 -0
- data/lib/bioroebe/fasta_and_fastq/fasta_defline/fasta_defline.rb +113 -0
- data/lib/bioroebe/fasta_and_fastq/fasta_parser.rb +167 -0
- data/lib/bioroebe/fasta_and_fastq/fastq_format_explainer.rb +131 -0
- data/lib/bioroebe/fasta_and_fastq/length_modifier/length_modifier.rb +87 -0
- data/lib/bioroebe/fasta_and_fastq/parse_fasta/constants.rb +50 -0
- data/lib/bioroebe/fasta_and_fastq/parse_fasta/initialize.rb +86 -0
- data/lib/bioroebe/fasta_and_fastq/parse_fasta/menu.rb +117 -0
- data/lib/bioroebe/fasta_and_fastq/parse_fasta/misc.rb +981 -0
- data/lib/bioroebe/fasta_and_fastq/parse_fasta/parse_fasta.rb +27 -0
- data/lib/bioroebe/fasta_and_fastq/parse_fasta/report.rb +156 -0
- data/lib/bioroebe/fasta_and_fastq/parse_fasta/reset.rb +128 -0
- data/lib/bioroebe/fasta_and_fastq/parse_fasta/run.rb +20 -0
- data/lib/bioroebe/fasta_and_fastq/parse_fastq/parse_fastq.rb +83 -0
- data/lib/bioroebe/fasta_and_fastq/return_fasta_subsection_of_this_file.rb +112 -0
- data/lib/bioroebe/fasta_and_fastq/show_fasta_headers.rb +135 -0
- data/lib/bioroebe/fasta_and_fastq/show_fasta_statistics.rb +188 -0
- data/lib/bioroebe/fasta_and_fastq/simplify_fasta_header/simplify_fasta_header.rb +111 -0
- data/lib/bioroebe/fasta_and_fastq/split_this_fasta_file_into_chromosomes/constants.rb +26 -0
- data/lib/bioroebe/fasta_and_fastq/split_this_fasta_file_into_chromosomes/menu.rb +41 -0
- data/lib/bioroebe/fasta_and_fastq/split_this_fasta_file_into_chromosomes/misc.rb +23 -0
- data/lib/bioroebe/fasta_and_fastq/split_this_fasta_file_into_chromosomes/reset.rb +68 -0
- data/lib/bioroebe/fasta_and_fastq/split_this_fasta_file_into_chromosomes/split_this_fasta_file_into_chromosomes.rb +290 -0
- data/lib/bioroebe/genbank/README.md +1 -0
- data/lib/bioroebe/genbank/genbank_flat_file_format_generator.rb +275 -0
- data/lib/bioroebe/genbank/genbank_parser.rb +291 -0
- data/lib/bioroebe/gene/gene.rb +64 -0
- data/lib/bioroebe/genomes/genome_pattern.rb +165 -0
- data/lib/bioroebe/genomes/genome_retriever.rb +79 -0
- data/lib/bioroebe/gui/experimental/README.md +1 -0
- data/lib/bioroebe/gui/experimental/snapgene/snapgene.rb +147 -0
- data/lib/bioroebe/gui/gtk3/README.md +2 -0
- data/lib/bioroebe/gui/gtk3/alignment/alignment.rb +337 -0
- data/lib/bioroebe/gui/gtk3/aminoacid_composition/aminoacid_composition.rb +510 -0
- data/lib/bioroebe/gui/gtk3/aminoacid_composition/customized_dialog.rb +55 -0
- data/lib/bioroebe/gui/gtk3/anti_sense_strand/anti_sense_strand.config +6 -0
- data/lib/bioroebe/gui/gtk3/anti_sense_strand/anti_sense_strand.rb +29 -0
- data/lib/bioroebe/gui/gtk3/blosum_matrix_viewer/blosum_matrix_viewer.rb +196 -0
- data/lib/bioroebe/gui/gtk3/calculate_cell_numbers_of_bacteria/calculate_cell_numbers_of_bacteria.config +6 -0
- data/lib/bioroebe/gui/gtk3/calculate_cell_numbers_of_bacteria/calculate_cell_numbers_of_bacteria.rb +106 -0
- data/lib/bioroebe/gui/gtk3/controller/controller.rb +406 -0
- data/lib/bioroebe/gui/gtk3/dna_to_aminoacid_widget/dna_to_aminoacid_widget.rb +609 -0
- data/lib/bioroebe/gui/gtk3/dna_to_reverse_complement_widget/dna_to_reverse_complement_widget.config +6 -0
- data/lib/bioroebe/gui/gtk3/dna_to_reverse_complement_widget/dna_to_reverse_complement_widget.rb +189 -0
- data/lib/bioroebe/gui/gtk3/fasta_table_widget/fasta_table_widget.rb +245 -0
- data/lib/bioroebe/gui/gtk3/format_converter/format_converter.rb +346 -0
- data/lib/bioroebe/gui/gtk3/gene/gene.rb +182 -0
- data/lib/bioroebe/gui/gtk3/hamming_distance/hamming_distance.config +6 -0
- data/lib/bioroebe/gui/gtk3/hamming_distance/hamming_distance.rb +370 -0
- data/lib/bioroebe/gui/gtk3/levensthein_distance/levensthein_distance.config +6 -0
- data/lib/bioroebe/gui/gtk3/levensthein_distance/levensthein_distance.rb +175 -0
- data/lib/bioroebe/gui/gtk3/nucleotide_analyser/nucleotide_analyser.rb +428 -0
- data/lib/bioroebe/gui/gtk3/parse_pdb_file/parse_pdb_file.rb +342 -0
- data/lib/bioroebe/gui/gtk3/primer_design_widget/primer_design_widget.rb +580 -0
- data/lib/bioroebe/gui/gtk3/protein_to_DNA/protein_to_DNA.config +6 -0
- data/lib/bioroebe/gui/gtk3/protein_to_DNA/protein_to_DNA.rb +182 -0
- data/lib/bioroebe/gui/gtk3/random_sequence/random_sequence.rb +566 -0
- data/lib/bioroebe/gui/gtk3/restriction_enzymes/restriction_enzymes.config +6 -0
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- data/lib/bioroebe/gui/gtk3/show_codon_table/misc.rb +556 -0
- data/lib/bioroebe/gui/gtk3/show_codon_table/show_codon_table.rb +171 -0
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- data/lib/bioroebe/gui/libui/calculate_cell_numbers_of_bacteria/calculate_cell_numbers_of_bacteria.rb +60 -0
- data/lib/bioroebe/gui/libui/dna_to_aminoacid_widget/dna_to_aminoacid_widget.rb +161 -0
- data/lib/bioroebe/gui/libui/dna_to_reverse_complement_widget/dna_to_reverse_complement_widget.rb +76 -0
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- data/lib/bioroebe/gui/shared_code/calculate_cell_numbers_of_bacteria/calculate_cell_numbers_of_bacteria_module.rb +216 -0
- data/lib/bioroebe/gui/shared_code/dna_to_reverse_complement_widget/dna_to_reverse_complement_widget_module.rb +284 -0
- data/lib/bioroebe/gui/shared_code/levensthein_distance/levensthein_distance_module.rb +402 -0
- data/lib/bioroebe/gui/shared_code/protein_to_DNA/protein_to_DNA_module.rb +192 -0
- data/lib/bioroebe/gui/shared_code/show_codon_table/show_codon_table_module.rb +72 -0
- data/lib/bioroebe/gui/shared_code/show_codon_usage/show_codon_usage_module.rb +213 -0
- data/lib/bioroebe/gui/tk/aminoacid_composition/aminoacid_composition.rb +206 -0
- data/lib/bioroebe/gui/tk/blosum_matrix_viewer/blosum_matrix_viewer.rb +140 -0
- data/lib/bioroebe/gui/tk/hamming_distance/hamming_distance.rb +262 -0
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- data/lib/bioroebe/gui/tk/three_to_one/three_to_one.rb +199 -0
- data/lib/bioroebe/gui/unified_widgets/anti_sense_strand/anti_sense_strand.rb +519 -0
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- data/lib/bioroebe/nucleotides/show_nucleotide_sequence.rb +556 -0
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- data/lib/bioroebe/palindromes/palindrome_finder.rb +208 -0
- data/lib/bioroebe/palindromes/palindrome_generator.rb +272 -0
- data/lib/bioroebe/parsers/biolang_parser.rb +156 -0
- data/lib/bioroebe/parsers/blosum_parser.rb +222 -0
- data/lib/bioroebe/parsers/genbank_parser.rb +78 -0
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- data/lib/bioroebe/patterns/analyse_glycosylation_pattern.rb +149 -0
- data/lib/bioroebe/patterns/is_this_sequence_a_EGF2_pattern.rb +66 -0
- data/lib/bioroebe/patterns/profile_pattern.rb +182 -0
- data/lib/bioroebe/patterns/rgg_scanner.rb +160 -0
- data/lib/bioroebe/patterns/scan_for_repeat.rb +157 -0
- data/lib/bioroebe/pdb/download_this_pdb.rb +67 -0
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- data/lib/bioroebe/pdb/report_secondary_structures_from_this_pdb_file.rb +225 -0
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- data/lib/bioroebe/project/project.rb +264 -0
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- data/lib/bioroebe/regexes/README.md +2 -0
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- data/lib/bioroebe/toplevel_methods/calculate_n50_value.rb +57 -0
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- data/lib/bioroebe/toplevel_methods/complement.rb +58 -0
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- data/lib/bioroebe/toplevel_methods/leading_five_prime_and_trailing_three_prime.rb +101 -0
- data/lib/bioroebe/toplevel_methods/levensthein.rb +63 -0
- data/lib/bioroebe/toplevel_methods/log_directory.rb +109 -0
- data/lib/bioroebe/toplevel_methods/longest_common_substring.rb +55 -0
- data/lib/bioroebe/toplevel_methods/map_ncbi_entry_to_eutils_id.rb +88 -0
- data/lib/bioroebe/toplevel_methods/matches.rb +259 -0
- data/lib/bioroebe/toplevel_methods/misc.rb +432 -0
- data/lib/bioroebe/toplevel_methods/nucleotides.rb +715 -0
- data/lib/bioroebe/toplevel_methods/number_of_clones.rb +63 -0
- data/lib/bioroebe/toplevel_methods/open_in_browser.rb +77 -0
- data/lib/bioroebe/toplevel_methods/open_reading_frames.rb +236 -0
- data/lib/bioroebe/toplevel_methods/opn.rb +34 -0
- data/lib/bioroebe/toplevel_methods/palindromes.rb +127 -0
- data/lib/bioroebe/toplevel_methods/parse.rb +59 -0
- data/lib/bioroebe/toplevel_methods/phred_error_probability.rb +68 -0
- data/lib/bioroebe/toplevel_methods/rds.rb +24 -0
- data/lib/bioroebe/toplevel_methods/remove.rb +86 -0
- data/lib/bioroebe/toplevel_methods/return_source_code_of_this_method.rb +35 -0
- data/lib/bioroebe/toplevel_methods/return_subsequence_based_on_indices.rb +68 -0
- data/lib/bioroebe/toplevel_methods/rna_splicing.rb +73 -0
- data/lib/bioroebe/toplevel_methods/rnalfold.rb +69 -0
- data/lib/bioroebe/toplevel_methods/searching_and_finding.rb +116 -0
- data/lib/bioroebe/toplevel_methods/shuffleseq.rb +37 -0
- data/lib/bioroebe/toplevel_methods/statistics.rb +53 -0
- data/lib/bioroebe/toplevel_methods/sum_of_odd_integers.rb +62 -0
- data/lib/bioroebe/toplevel_methods/taxonomy.rb +187 -0
- data/lib/bioroebe/toplevel_methods/three_delimiter.rb +34 -0
- data/lib/bioroebe/toplevel_methods/time_and_date.rb +53 -0
- data/lib/bioroebe/toplevel_methods/to_camelcase.rb +26 -0
- data/lib/bioroebe/toplevel_methods/truncate.rb +48 -0
- data/lib/bioroebe/toplevel_methods/url.rb +36 -0
- data/lib/bioroebe/toplevel_methods/verbose.rb +59 -0
- data/lib/bioroebe/utility_scripts/align_open_reading_frames.rb +191 -0
- data/lib/bioroebe/utility_scripts/analyse_local_dataset.rb +119 -0
- data/lib/bioroebe/utility_scripts/check_for_mismatches/check_for_mismatches.rb +230 -0
- data/lib/bioroebe/utility_scripts/compacter.rb +131 -0
- data/lib/bioroebe/utility_scripts/compseq/compseq.rb +529 -0
- data/lib/bioroebe/utility_scripts/consensus_sequence.rb +374 -0
- data/lib/bioroebe/utility_scripts/create_batch_entrez_file.rb +130 -0
- data/lib/bioroebe/utility_scripts/determine_antigenic_areas.rb +115 -0
- data/lib/bioroebe/utility_scripts/determine_missing_nucleotides_percentage.rb +137 -0
- data/lib/bioroebe/utility_scripts/display_open_reading_frames/determine.rb +73 -0
- data/lib/bioroebe/utility_scripts/display_open_reading_frames/display_open_reading_frames.rb +31 -0
- data/lib/bioroebe/utility_scripts/display_open_reading_frames/initialize.rb +37 -0
- data/lib/bioroebe/utility_scripts/display_open_reading_frames/menu.rb +49 -0
- data/lib/bioroebe/utility_scripts/display_open_reading_frames/misc.rb +471 -0
- data/lib/bioroebe/utility_scripts/display_open_reading_frames/report.rb +113 -0
- data/lib/bioroebe/utility_scripts/display_open_reading_frames/reset.rb +56 -0
- data/lib/bioroebe/utility_scripts/dot_alignment.rb +177 -0
- data/lib/bioroebe/utility_scripts/download_files_from_rebase.rb +72 -0
- data/lib/bioroebe/utility_scripts/find_gene.rb +202 -0
- data/lib/bioroebe/utility_scripts/mirror_repeat.rb +235 -0
- data/lib/bioroebe/utility_scripts/move_file_to_its_correct_location.rb +151 -0
- data/lib/bioroebe/utility_scripts/parse_taxonomy.rb +168 -0
- data/lib/bioroebe/utility_scripts/pathways.rb +152 -0
- data/lib/bioroebe/utility_scripts/permutations.rb +145 -0
- data/lib/bioroebe/utility_scripts/punnet/punnet.rb +126 -0
- data/lib/bioroebe/utility_scripts/show_this_dna_sequence.rb +148 -0
- data/lib/bioroebe/utility_scripts/showorf/constants.rb +36 -0
- data/lib/bioroebe/utility_scripts/showorf/help.rb +33 -0
- data/lib/bioroebe/utility_scripts/showorf/initialize.rb +52 -0
- data/lib/bioroebe/utility_scripts/showorf/menu.rb +68 -0
- data/lib/bioroebe/utility_scripts/showorf/reset.rb +39 -0
- data/lib/bioroebe/utility_scripts/showorf/run.rb +152 -0
- data/lib/bioroebe/utility_scripts/showorf/show.rb +97 -0
- data/lib/bioroebe/utility_scripts/showorf/showorf.rb +488 -0
- data/lib/bioroebe/version/version.rb +44 -0
- data/lib/bioroebe/viennarna/README.md +3 -0
- data/lib/bioroebe/viennarna/rnafold_wrapper.rb +196 -0
- data/lib/bioroebe/with_gui.rb +18 -0
- data/lib/bioroebe/www/bioroebe.cgi +44 -0
- data/lib/bioroebe/www/embeddable_interface.rb +686 -0
- data/lib/bioroebe/www/sinatra/sinatra.rb +1013 -0
- data/lib/bioroebe/yaml/agarose/agarose_concentrations.yml +21 -0
- data/lib/bioroebe/yaml/aminoacids/amino_acids.yml +92 -0
- data/lib/bioroebe/yaml/aminoacids/amino_acids_abbreviations.yml +31 -0
- data/lib/bioroebe/yaml/aminoacids/amino_acids_average_mass_table.yml +33 -0
- data/lib/bioroebe/yaml/aminoacids/amino_acids_classification.yml +18 -0
- data/lib/bioroebe/yaml/aminoacids/amino_acids_english.yml +26 -0
- data/lib/bioroebe/yaml/aminoacids/amino_acids_frequency.yml +44 -0
- data/lib/bioroebe/yaml/aminoacids/amino_acids_long_name_to_one_letter.yml +61 -0
- data/lib/bioroebe/yaml/aminoacids/amino_acids_molecular_formula.yml +32 -0
- data/lib/bioroebe/yaml/aminoacids/amino_acids_monoisotopic_mass_table.yml +38 -0
- data/lib/bioroebe/yaml/aminoacids/amino_acids_reste.yml +35 -0
- data/lib/bioroebe/yaml/aminoacids/amino_acids_three_to_one.yml +34 -0
- data/lib/bioroebe/yaml/aminoacids/hydropathy_table.yml +44 -0
- data/lib/bioroebe/yaml/aminoacids/molecular_weight.yml +29 -0
- data/lib/bioroebe/yaml/aminoacids/simple_aminoacids.yml +66 -0
- data/lib/bioroebe/yaml/aminoacids/weight_of_common_proteins.yml +33 -0
- data/lib/bioroebe/yaml/antisense/antisense.yml +9 -0
- data/lib/bioroebe/yaml/base_composition_of_dna.yml +37 -0
- data/lib/bioroebe/yaml/blosum/blosum45.yml +36 -0
- data/lib/bioroebe/yaml/blosum/blosum50.yml +34 -0
- data/lib/bioroebe/yaml/blosum/blosum62.yml +35 -0
- data/lib/bioroebe/yaml/blosum/blosum80.yml +37 -0
- data/lib/bioroebe/yaml/blosum/blosum90.yml +36 -0
- data/lib/bioroebe/yaml/blosum/blosum_matrix.yml +200 -0
- data/lib/bioroebe/yaml/chromosomes/chromosome_numbers.yml +30 -0
- data/lib/bioroebe/yaml/codon_tables/1.yml +113 -0
- data/lib/bioroebe/yaml/codon_tables/10.yml +89 -0
- data/lib/bioroebe/yaml/codon_tables/11.yml +91 -0
- data/lib/bioroebe/yaml/codon_tables/12.yml +89 -0
- data/lib/bioroebe/yaml/codon_tables/13.yml +89 -0
- data/lib/bioroebe/yaml/codon_tables/14.yml +89 -0
- data/lib/bioroebe/yaml/codon_tables/15.yml +94 -0
- data/lib/bioroebe/yaml/codon_tables/16.yml +89 -0
- data/lib/bioroebe/yaml/codon_tables/2.yml +86 -0
- data/lib/bioroebe/yaml/codon_tables/21.yml +89 -0
- data/lib/bioroebe/yaml/codon_tables/22.yml +89 -0
- data/lib/bioroebe/yaml/codon_tables/23.yml +91 -0
- data/lib/bioroebe/yaml/codon_tables/24.yml +89 -0
- data/lib/bioroebe/yaml/codon_tables/25.yml +89 -0
- data/lib/bioroebe/yaml/codon_tables/26.yml +96 -0
- data/lib/bioroebe/yaml/codon_tables/27.yml +104 -0
- data/lib/bioroebe/yaml/codon_tables/28.yml +97 -0
- data/lib/bioroebe/yaml/codon_tables/29.yml +93 -0
- data/lib/bioroebe/yaml/codon_tables/3.yml +98 -0
- data/lib/bioroebe/yaml/codon_tables/30.yml +91 -0
- data/lib/bioroebe/yaml/codon_tables/31.yml +94 -0
- data/lib/bioroebe/yaml/codon_tables/33.yml +101 -0
- data/lib/bioroebe/yaml/codon_tables/4.yml +96 -0
- data/lib/bioroebe/yaml/codon_tables/5.yml +100 -0
- data/lib/bioroebe/yaml/codon_tables/6.yml +96 -0
- data/lib/bioroebe/yaml/codon_tables/9.yml +97 -0
- data/lib/bioroebe/yaml/codon_tables/overview.yml +42 -0
- data/lib/bioroebe/yaml/configuration/README.md +12 -0
- data/lib/bioroebe/yaml/configuration/browser.yml +1 -0
- data/lib/bioroebe/yaml/configuration/colourize_fasta_sequences.yml +14 -0
- data/lib/bioroebe/yaml/configuration/default_colours_for_the_aminoacids.yml +28 -0
- data/lib/bioroebe/yaml/configuration/temp_dir.yml +1 -0
- data/lib/bioroebe/yaml/configuration/try_to_use_matplotlib.yml +1 -0
- data/lib/bioroebe/yaml/configuration/use_opn.yml +1 -0
- data/lib/bioroebe/yaml/configuration/use_this_database.yml +1 -0
- data/lib/bioroebe/yaml/create_these_directories_on_startup/create_these_directories_on_startup.yml +9 -0
- data/lib/bioroebe/yaml/default_dna_input.yml +3 -0
- data/lib/bioroebe/yaml/enzymes/enzyme_classes.yml +15 -0
- data/lib/bioroebe/yaml/enzymes/pH-Optima.yml +11 -0
- data/lib/bioroebe/yaml/fasta_and_fastq/fastq_quality_schemes.yml +44 -0
- data/lib/bioroebe/yaml/genomes/README.md +16 -0
- data/lib/bioroebe/yaml/humans/README.md +2 -0
- data/lib/bioroebe/yaml/humans/human_chromosomes.yml +53 -0
- data/lib/bioroebe/yaml/laboratory/README.md +1 -0
- data/lib/bioroebe/yaml/laboratory/pipettes.yml +8 -0
- data/lib/bioroebe/yaml/mRNA/mRNA.yml +16 -0
- data/lib/bioroebe/yaml/nuclear_localization_sequences.yml +15 -0
- data/lib/bioroebe/yaml/nucleotides/abbreviations_for_nucleotides.yml +29 -0
- data/lib/bioroebe/yaml/nucleotides/nucleotide_density.yml +10 -0
- data/lib/bioroebe/yaml/nucleotides/nucleotides.yml +34 -0
- data/lib/bioroebe/yaml/nucleotides/nucleotides_weight.yml +12 -0
- data/lib/bioroebe/yaml/pathways/README.md +2 -0
- data/lib/bioroebe/yaml/pathways/citric_acid_cycle.yml +16 -0
- data/lib/bioroebe/yaml/pathways/glycolysis.yml +20 -0
- data/lib/bioroebe/yaml/pathways/shikimate_pathway.yml +23 -0
- data/lib/bioroebe/yaml/pathways/urea_cycle.yml +11 -0
- data/lib/bioroebe/yaml/primers/README.md +4 -0
- data/lib/bioroebe/yaml/primers/primers.yml +3 -0
- data/lib/bioroebe/yaml/promoters/35S.yml +15 -0
- data/lib/bioroebe/yaml/promoters/strong_promoters.yml +24 -0
- data/lib/bioroebe/yaml/proteases/proteases.yml +3 -0
- data/lib/bioroebe/yaml/proteins/ubiquitin.yml +4 -0
- data/lib/bioroebe/yaml/remote_urls/README.md +4 -0
- data/lib/bioroebe/yaml/remote_urls/remote_urls.yml +3 -0
- data/lib/bioroebe/yaml/restriction_enzymes/restriction_enzymes.yml +630 -0
- data/lib/bioroebe/yaml/sequences/JX472995_Green_fluorescent_protein_from_Aequorea_victoria.fasta +14 -0
- data/lib/bioroebe/yaml/sequences/README.md +2 -0
- data/lib/bioroebe/yaml/talens.yml +22 -0
- data/lib/bioroebe/yaml/viruses/ecoli_phages.yml +63 -0
- data/lib/bioroebe/yaml/viruses/viruses.yml +6 -0
- data/lib/bioroebe.rb +5 -0
- data/spec/testing_toplevel_method_editor.rb +20 -0
- data/spec/testing_toplevel_method_url.rb +15 -0
- data/spec/testing_toplevel_method_verbose.rb +13 -0
- data/test/advanced_svg_example.rb +307 -0
- data/test/testing_bioroebe.rb +25 -0
- data/test/testing_codons.rb +45 -0
- data/test/testing_dna_to_rna_conversion.rb +15 -0
- data/test/testing_parse_pdb_file.rb +23 -0
- data/test/testing_reverse_complement.rb +32 -0
- data/test/testing_svg_component_of_bioroebe.rb +311 -0
- data/test/testing_svg_component_of_bioroebe_from_json_dataset.rb +34 -0
- data/test/testing_taxonomy.rb +22 -0
- metadata +1059 -0
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#!/usr/bin/ruby -w
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# Encoding: UTF-8
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# =========================================================================== #
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# === Bioroebe::DnaToAminoacidSequence
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#
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# This class will convert between DNA and the corresponding aminoacid
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# sequence associated with that DNA sequence. The input given to this
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# class will be assumed to be the DNA sequence at hand.
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#
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# Each DNA sequence is read in as a codon, aka 3 consecutive nucleotides.
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#
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#
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#
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# Examples:
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#
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#
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# Usage example:
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#
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# =========================================================================== #
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# require 'bioroebe/conversions/dna_to_aminoacid_sequence.rb'
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# Bioroebe::DnaToAminoacidSequence.new(ARGV)
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# =========================================================================== #
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require 'bioroebe/base/commandline_application/commandline_application.rb'
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module Bioroebe
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class DnaToAminoacidSequence < ::Bioroebe::CommandlineApplication # === Bioroebe::DnaToAminoacidSequence
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require 'bioroebe/codons/codon_table.rb'
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# === initialize
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def initialize(
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)
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reset
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set_commandline_arguments(
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commandline_arguments
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be_quiet
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end
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end
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:two
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set_use_frame_number(:two)
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:three
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set_use_frame_number(:three)
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# ======================================================================= #
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# === :be_quiet_and_no_colours_and_no_output,
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:return_silently
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be_quiet
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disable_colours
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no_output
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# ======================================================================= #
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when :be_quiet_and_no_colours
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be_quiet
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disable_colours
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# ======================================================================= #
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# === :be_quiet
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# ======================================================================= #
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be_quiet
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run if run_already
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end
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# ========================================================================= #
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def reset
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super()
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set_use_frame_number(:one) # Default is reading frame number one.
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# ======================================================================= #
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# === @codon_table_dataset
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#
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# Obtain the codon table dataset next.
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@codon_table_dataset = ::Bioroebe.codon_table_dataset?
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# ======================================================================= #
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# === @result
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@result = nil
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# ======================================================================= #
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# === @use_the_codon_table_for
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#
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# the codon table for humans.
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# ======================================================================= #
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@use_the_codon_table_for = :humans
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# ======================================================================= #
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# === @colourize_stop_codons
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#
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# If the following variable is set to true then any stop codon, aka
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# '*', will appear in the colour orchid.
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@colourize_stop_codons = true
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# ======================================================================= #
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# === @internal_hash
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# ======================================================================= #
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|
+
@internal_hash = {}
|
153
|
+
# ======================================================================= #
|
154
|
+
# === :report_result
|
155
|
+
# ======================================================================= #
|
156
|
+
@internal_hash[:report_result] = true
|
157
|
+
# ======================================================================= #
|
158
|
+
# === :show_as_three_letter_aminoacid_sequence
|
159
|
+
#
|
160
|
+
# If this is true then this class will show "Phe" rather than "F" for
|
161
|
+
# that amino acid. The same is true for all the other aminoacids as well.
|
162
|
+
# ======================================================================= #
|
163
|
+
@internal_hash[:show_as_three_letter_aminoacid_sequence] = false
|
164
|
+
end
|
165
|
+
|
166
|
+
# ========================================================================= #
|
167
|
+
# === do_show_as_the_three_letter_aminoacid_sequence
|
168
|
+
# ========================================================================= #
|
169
|
+
def do_show_as_the_three_letter_aminoacid_sequence
|
170
|
+
@internal_hash[:show_as_three_letter_aminoacid_sequence] = true
|
171
|
+
end
|
172
|
+
|
173
|
+
# ========================================================================= #
|
174
|
+
# === show_as_three_letter_aminoacid_sequence?
|
175
|
+
# ========================================================================= #
|
176
|
+
def show_as_three_letter_aminoacid_sequence?
|
177
|
+
@internal_hash[:show_as_three_letter_aminoacid_sequence]
|
178
|
+
end
|
179
|
+
|
180
|
+
# ========================================================================= #
|
181
|
+
# === set_use_the_codon_table_for
|
182
|
+
# ========================================================================= #
|
183
|
+
def set_use_the_codon_table_for(i)
|
184
|
+
@use_the_codon_table_for = i
|
185
|
+
end
|
186
|
+
|
187
|
+
# ========================================================================= #
|
188
|
+
# === be_quiet
|
189
|
+
# ========================================================================= #
|
190
|
+
def be_quiet
|
191
|
+
@be_verbose = false
|
192
|
+
@internal_hash[:report_result] = false
|
193
|
+
end
|
194
|
+
|
195
|
+
# ========================================================================= #
|
196
|
+
# === be_quiet_and_no_colours
|
197
|
+
# ========================================================================= #
|
198
|
+
def be_quiet_and_no_colours
|
199
|
+
be_quiet
|
200
|
+
disable_colours
|
201
|
+
end
|
202
|
+
|
203
|
+
# ========================================================================= #
|
204
|
+
# === one_two_or_three
|
205
|
+
# ========================================================================= #
|
206
|
+
def one_two_or_three(i)
|
207
|
+
case i
|
208
|
+
when :frame_one
|
209
|
+
'1'
|
210
|
+
when :frame_two
|
211
|
+
'2'
|
212
|
+
when :frame_three
|
213
|
+
'3'
|
214
|
+
end
|
215
|
+
end
|
216
|
+
|
217
|
+
# ========================================================================= #
|
218
|
+
# === set_use_frame_number
|
219
|
+
#
|
220
|
+
# Designate which frame is to be used through this method. By default
|
221
|
+
# it will be frame number 1.
|
222
|
+
# ========================================================================= #
|
223
|
+
def set_use_frame_number(i = :default)
|
224
|
+
case i
|
225
|
+
# ======================================================================= #
|
226
|
+
# First comes the default.
|
227
|
+
# ======================================================================= #
|
228
|
+
when :one,
|
229
|
+
:default,
|
230
|
+
'1'
|
231
|
+
i = :frame_one
|
232
|
+
when :two,
|
233
|
+
'2'
|
234
|
+
i = :frame_two
|
235
|
+
when :three,
|
236
|
+
'3'
|
237
|
+
i = :frame_three
|
238
|
+
end
|
239
|
+
@use_frame_number = i
|
240
|
+
end; alias use_this_reading_frame= set_use_frame_number # === use_this_reading_frame=
|
241
|
+
alias set_use_this_reading_frame set_use_frame_number # === set_use_this_reading_frame
|
242
|
+
|
243
|
+
# ========================================================================= #
|
244
|
+
# === report_result?
|
245
|
+
# ========================================================================= #
|
246
|
+
def report_result?
|
247
|
+
@internal_hash[:report_result]
|
248
|
+
end
|
249
|
+
|
250
|
+
# ========================================================================= #
|
251
|
+
# === no_output
|
252
|
+
# ========================================================================= #
|
253
|
+
def no_output
|
254
|
+
@internal_hash[:report_result] = false
|
255
|
+
end
|
256
|
+
|
257
|
+
# ========================================================================= #
|
258
|
+
# === set_result
|
259
|
+
# ========================================================================= #
|
260
|
+
def set_result(i)
|
261
|
+
i = i.join if i.is_a? Array
|
262
|
+
i.delete!('|') if i.include? '|'
|
263
|
+
@result = i
|
264
|
+
end; alias use_this_sequence set_result # === use_this_sequence
|
265
|
+
alias use_this_sequence= set_result # === use_this_sequence=
|
266
|
+
alias set_sequence set_result # === use_this_sequence=
|
267
|
+
|
268
|
+
# ========================================================================= #
|
269
|
+
# === report
|
270
|
+
# ========================================================================= #
|
271
|
+
def report
|
272
|
+
if be_verbose?
|
273
|
+
e
|
274
|
+
coloured_frame = palegreen(
|
275
|
+
'frame-'+one_two_or_three(@use_frame_number)
|
276
|
+
)+rev
|
277
|
+
e "#{rev}The #{coloured_frame}#{rev} sequence is: (#{sequence?.size} aminoacids)"
|
278
|
+
e
|
279
|
+
end
|
280
|
+
do_report_the_reading_frame
|
281
|
+
end
|
282
|
+
|
283
|
+
# ========================================================================= #
|
284
|
+
# === do_report_the_reading_frame
|
285
|
+
#
|
286
|
+
# This method will, with colours, output the full reading frame.
|
287
|
+
# ========================================================================= #
|
288
|
+
def do_report_the_reading_frame(
|
289
|
+
i = @result
|
290
|
+
)
|
291
|
+
# ======================================================================= #
|
292
|
+
# Always assign to @result next.
|
293
|
+
# ======================================================================= #
|
294
|
+
if show_as_three_letter_aminoacid_sequence?
|
295
|
+
i = one_to_three(i)
|
296
|
+
end
|
297
|
+
result = "#{lightgreen(i)}#{rev}"
|
298
|
+
if @colourize_stop_codons and result.include?('*')
|
299
|
+
result = result.dup if result.frozen?
|
300
|
+
# ===================================================================== #
|
301
|
+
# Colourize stop codons in orchid.
|
302
|
+
# ===================================================================== #
|
303
|
+
result.gsub!(
|
304
|
+
/\*/,
|
305
|
+
orchid('*')+
|
306
|
+
remove_trailing_ansii_escape_code(lightgreen(''))
|
307
|
+
)
|
308
|
+
end
|
309
|
+
set_result(result)
|
310
|
+
if report_result? and @be_verbose
|
311
|
+
e " #{@result}"
|
312
|
+
e N
|
313
|
+
end
|
314
|
+
end
|
315
|
+
|
316
|
+
# ========================================================================= #
|
317
|
+
# === result?
|
318
|
+
#
|
319
|
+
# This variable will hold the translated sequence ready, e. g. the
|
320
|
+
# aminoacid sequence.
|
321
|
+
# ========================================================================= #
|
322
|
+
def result?
|
323
|
+
@result
|
324
|
+
end; alias result result? # === result
|
325
|
+
alias input_sequence? result? # === input_sequence?
|
326
|
+
alias seq? result? # === seq?
|
327
|
+
alias sequence? result? # === sequence?
|
328
|
+
alias sequence result? # === sequence
|
329
|
+
|
330
|
+
# ========================================================================= #
|
331
|
+
# === translate_dna_into_aminoacid
|
332
|
+
#
|
333
|
+
# This method will perform the lookup onto the hash (codon table dataset),
|
334
|
+
# in order to find the corresponding aminoacid.
|
335
|
+
# ========================================================================= #
|
336
|
+
def translate_dna_into_aminoacid(i)
|
337
|
+
@codon_table_dataset[
|
338
|
+
i.tr('U','T')
|
339
|
+
]
|
340
|
+
end
|
341
|
+
|
342
|
+
# ========================================================================= #
|
343
|
+
# === show_help (help tag)
|
344
|
+
# ========================================================================= #
|
345
|
+
def show_help
|
346
|
+
e 'Available options:'
|
347
|
+
e
|
348
|
+
e ' --gui # start the GUI'
|
349
|
+
e ' --stop-at-the-first-stop-codon # stop at the first stop codon encountered'
|
350
|
+
e ' --frame1 # show the first frame translation only'
|
351
|
+
e ' --frame2 # show the second frame translation only'
|
352
|
+
e ' --frame3 # show the third frame translation only'
|
353
|
+
e ' --use-this-codon-table=bacteria # specify a specific codon table '\
|
354
|
+
'(in this case bacteria)'
|
355
|
+
e ' --three-letter # show the three letter amino acid code'
|
356
|
+
e
|
357
|
+
end
|
358
|
+
|
359
|
+
# ========================================================================= #
|
360
|
+
# === menu (menu tag)
|
361
|
+
#
|
362
|
+
# These are the registered commandline arguments for this class.
|
363
|
+
#
|
364
|
+
# We will act only on arguments given to this method, staring with
|
365
|
+
# two hyphens (aka --).
|
366
|
+
# ========================================================================= #
|
367
|
+
def menu(
|
368
|
+
i = return_commandline_arguments_starting_with_two_hyphens
|
369
|
+
)
|
370
|
+
if i.is_a? Array
|
371
|
+
i.each {|entry| menu(entry) }
|
372
|
+
else
|
373
|
+
case i
|
374
|
+
# ===================================================================== #
|
375
|
+
# === Show the aminoacids as three-letter code
|
376
|
+
#
|
377
|
+
# Usage example:
|
378
|
+
#
|
379
|
+
# dnatoaminoacidsequence AUGUUU --three-letter
|
380
|
+
#
|
381
|
+
# ===================================================================== #
|
382
|
+
when /^-?-?three(-|_| )?letter$/i
|
383
|
+
do_show_as_the_three_letter_aminoacid_sequence
|
384
|
+
# ===================================================================== #
|
385
|
+
# === Show the help section
|
386
|
+
#
|
387
|
+
# Usage example:
|
388
|
+
#
|
389
|
+
# dnatoaminoacidsequence --help
|
390
|
+
#
|
391
|
+
# ===================================================================== #
|
392
|
+
when /^-?-?help$/i
|
393
|
+
show_help
|
394
|
+
exit
|
395
|
+
# ===================================================================== #
|
396
|
+
# === Start the GUI component
|
397
|
+
#
|
398
|
+
# Usage example:
|
399
|
+
#
|
400
|
+
# dnatoaminoacidsequence --gui
|
401
|
+
#
|
402
|
+
# ===================================================================== #
|
403
|
+
when /^-?-?gui$/i
|
404
|
+
require 'bioroebe/gui/gtk3/dna_to_aminoacid_widget/dna_to_aminoacid_widget.rb'
|
405
|
+
::Bioroebe::GUI::Gtk::DnaToAminoacidWidget.run
|
406
|
+
exit
|
407
|
+
# ===================================================================== #
|
408
|
+
# === Stop at the first stop codon
|
409
|
+
#
|
410
|
+
# This entry point allows us to stop at the first found stop codon.
|
411
|
+
#
|
412
|
+
# Normally the whole aminoacid sequence would be shown, but via
|
413
|
+
# the --stop-at-the-first-stop-codon flag we can stop right at
|
414
|
+
# the first found '*' token.
|
415
|
+
#
|
416
|
+
# Usage example:
|
417
|
+
#
|
418
|
+
# dna_to_aminoacid_sequence ATGATGCCTCCAGGG --stop-at-the-first-stop-codon
|
419
|
+
#
|
420
|
+
# ===================================================================== #
|
421
|
+
when /^-?-?stop(-|_| )?at(-|_| )?the(-|_| )?first(-|_| )?stop(-|_| )?codon$/i
|
422
|
+
@stop_at_the_first_stop_codon = true
|
423
|
+
# ===================================================================== #
|
424
|
+
# === Use a specific frame
|
425
|
+
#
|
426
|
+
# This entry point can be used to designate a specific frame,
|
427
|
+
# such as frame2 or frame3. (Specifying frame1 won't make
|
428
|
+
# a whole lot of sense, though, but it exists primarily for
|
429
|
+
# debugging purposes).
|
430
|
+
#
|
431
|
+
# Usage example:
|
432
|
+
#
|
433
|
+
# dna_to_aminoacid_sequence ATGATGCCTCCAGGG --frame1
|
434
|
+
# dna_to_aminoacid_sequence ATGATGCCTCCAGGG --frame2
|
435
|
+
# dna_to_aminoacid_sequence ATGATGCCTCCAGGG --frame3
|
436
|
+
#
|
437
|
+
# ===================================================================== #
|
438
|
+
when /^-?-?frame(\d{1})$/i
|
439
|
+
set_use_this_reading_frame($1.to_s.dup)
|
440
|
+
# ===================================================================== #
|
441
|
+
# === dna_to_aminoacid_sequence ATGATGCCTCCA
|
442
|
+
#
|
443
|
+
# Usage example:
|
444
|
+
#
|
445
|
+
# dna_to_aminoacid_sequence ATGATGCCTCCAGGG --use-this-codon-table=bacteria
|
446
|
+
# dna_to_aminoacid_sequence ATGATGCCTCCAGGG --use-this-codon-table=humans
|
447
|
+
#
|
448
|
+
# ===================================================================== #
|
449
|
+
when /^-?-?use(-|_| )?this(-|_| )?codon(-|_| )?table=(.+)$/i # === $4
|
450
|
+
_ = $4.to_s.dup.to_sym
|
451
|
+
set_use_the_codon_table_for(_)
|
452
|
+
end
|
453
|
+
end
|
454
|
+
end
|
455
|
+
|
456
|
+
# ========================================================================= #
|
457
|
+
# === do_the_conversion_step
|
458
|
+
#
|
459
|
+
# This method will do the actual DNA-to-aminoacid conversion step.
|
460
|
+
#
|
461
|
+
# Make sure that the correct reading frame has been "selected" before
|
462
|
+
# calling this method.
|
463
|
+
# ========================================================================= #
|
464
|
+
def do_the_conversion_step
|
465
|
+
# ======================================================================= #
|
466
|
+
# Use the proper, designated codon-table.
|
467
|
+
# ======================================================================= #
|
468
|
+
::Bioroebe.load_the_codon_table_dataset(@use_the_codon_table_for)
|
469
|
+
_ = first_argument?.dup
|
470
|
+
if _ and _.include?('.') and File.file?(_)
|
471
|
+
_ = File.read(_).strip # Add support for reading local files here.
|
472
|
+
end
|
473
|
+
# ======================================================================= #
|
474
|
+
# Here we have to honour the correct reading frame:
|
475
|
+
# ======================================================================= #
|
476
|
+
case @use_frame_number
|
477
|
+
when :frame_two
|
478
|
+
_[0,1] = ''
|
479
|
+
when :frame_three
|
480
|
+
_[0,2] = ''
|
481
|
+
end
|
482
|
+
unless _
|
483
|
+
erev 'Please supply a sequence to this class.'
|
484
|
+
exit
|
485
|
+
end
|
486
|
+
splitted = _.scan(/.../)
|
487
|
+
@result = ''.dup
|
488
|
+
splitted.each {|this_codon|
|
489
|
+
# ===================================================================== #
|
490
|
+
# === Do convert the DNA sequence into the corresponding aminoacid
|
491
|
+
# sequence next.
|
492
|
+
#
|
493
|
+
# Find the corresponding aminoacid next, by looking at the
|
494
|
+
# codon_table_dataset. We will replace all 'U' with 'T' to
|
495
|
+
# support RNA as input as well, just in case.
|
496
|
+
#
|
497
|
+
# The result is stored in the variable called @result.
|
498
|
+
# ===================================================================== #
|
499
|
+
translated_codon = translate_dna_into_aminoacid(this_codon)
|
500
|
+
@result << translated_codon.to_s
|
501
|
+
if @stop_at_the_first_stop_codon and (translated_codon == '*')
|
502
|
+
break
|
503
|
+
end
|
504
|
+
}
|
505
|
+
end
|
506
|
+
|
507
|
+
# ========================================================================= #
|
508
|
+
# === run (run tag)
|
509
|
+
# ========================================================================= #
|
510
|
+
def run
|
511
|
+
menu
|
512
|
+
do_the_conversion_step
|
513
|
+
report
|
514
|
+
end
|
515
|
+
|
516
|
+
# ========================================================================= #
|
517
|
+
# === Bioroebe::DnaToAminoacidSequence[]
|
518
|
+
# ========================================================================= #
|
519
|
+
def self.[](i = '')
|
520
|
+
new(i) { :be_quiet }.result
|
521
|
+
end
|
522
|
+
|
523
|
+
end
|
524
|
+
|
525
|
+
# =========================================================================== #
|
526
|
+
# === Bioroebe.dna_to_aminoacid_sequence
|
527
|
+
#
|
528
|
+
# Usage example:
|
529
|
+
#
|
530
|
+
# Bioroebe.dna_to_aminoacid_sequence('ATGGGGCCC') # => "MGP"
|
531
|
+
#
|
532
|
+
# =========================================================================== #
|
533
|
+
def self.dna_to_aminoacid_sequence(
|
534
|
+
i = ARGV
|
535
|
+
)
|
536
|
+
::Bioroebe::DnaToAminoacidSequence.new(i) { :be_quiet }.sequence?
|
537
|
+
end; self.instance_eval { alias dna_to_aa dna_to_aminoacid_sequence } # === Bioroebe.dna_to_aa
|
538
|
+
# self.instance_eval { alias codon_to_aminoacid dna_to_aminoacid_sequence } # === Bioroebe.codon_to_aminoacid
|
539
|
+
# ^^^ Disabled the above as of June 2021, due to colour output.
|
540
|
+
|
541
|
+
# =========================================================================== #
|
542
|
+
# === Bioroebe.translate_all_three_frames
|
543
|
+
# =========================================================================== #
|
544
|
+
def self.translate_all_three_frames(i)
|
545
|
+
::Bioroebe::DnaToAminoacidSequence.new(i) { :be_verbose }
|
546
|
+
::Bioroebe::DnaToAminoacidSequence.new(i, :frame_two) { :be_verbose }
|
547
|
+
::Bioroebe::DnaToAminoacidSequence.new(i, :frame_three) { :be_verbose }
|
548
|
+
end
|
549
|
+
|
550
|
+
end # dnatoaminoacidsequence ATGATGCCTCCAGGGUAAATGCCTCCAGGGUAAGGC
|
551
|
+
|
552
|
+
if __FILE__ == $PROGRAM_NAME
|
553
|
+
alias e puts
|
554
|
+
require 'bioroebe/colours/rev.rb'
|
555
|
+
_ = ARGV
|
556
|
+
if _.empty?
|
557
|
+
_ = 'ATGCGTAAAGGAGAAGAACTTTTCACTTGGAGTTGTCCCAATTCTTGTTGGGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGGTTATGGTGTTCAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATGAACTATACAAATAG'
|
558
|
+
end
|
559
|
+
Bioroebe.erev 'The input-sequence was: '+
|
560
|
+
_.join
|
561
|
+
Bioroebe::DnaToAminoacidSequence.new(_)
|
562
|
+
e
|
563
|
+
e 'Next, all three frames will be translated:'
|
564
|
+
e
|
565
|
+
Bioroebe.translate_all_three_frames(_)
|
566
|
+
end # dnatoaminoacid_sequence ATGATGCCTCCA
|
567
|
+
# topleveltranseq ATGGGCGGGAAATATTTA
|
568
|
+
# topleveltranseq ATGCATGCAAAA
|
569
|
+
# topleveltranseq "ATG|GGC|GGG|AAA|TAT|TTA|TAG"
|
@@ -0,0 +1 @@
|
|
1
|
+
All count-related files should be gathered here.
|