@pikaa-ai/pikaa 0.3.1 → 0.3.4
This diff represents the content of publicly available package versions that have been released to one of the supported registries. The information contained in this diff is provided for informational purposes only and reflects changes between package versions as they appear in their respective public registries.
- package/dist/cli.js +1169 -601
- package/dist/index.js +525 -204
- package/package.json +5 -3
- package/skills/adaptyv/references/api-endpoints.md +0 -690
- package/skills/aeon/references/anomaly_detection.md +0 -154
- package/skills/aeon/references/classification.md +0 -144
- package/skills/aeon/references/clustering.md +0 -123
- package/skills/aeon/references/datasets_benchmarking.md +0 -392
- package/skills/aeon/references/distances.md +0 -256
- package/skills/aeon/references/forecasting.md +0 -109
- package/skills/aeon/references/networks.md +0 -289
- package/skills/aeon/references/regression.md +0 -118
- package/skills/aeon/references/segmentation.md +0 -163
- package/skills/aeon/references/similarity_search.md +0 -187
- package/skills/aeon/references/transformations.md +0 -246
- package/skills/analytical-method-validation/assets/validation-protocol-template.md +0 -106
- package/skills/analytical-method-validation/assets/validation-report-template.md +0 -116
- package/skills/analytical-method-validation/references/compendial-and-clsi.md +0 -96
- package/skills/analytical-method-validation/references/framework-selection.md +0 -87
- package/skills/analytical-method-validation/references/ich-m10-bioanalytical.md +0 -123
- package/skills/analytical-method-validation/references/ich-q2r2.md +0 -229
- package/skills/analytical-method-validation/references/source-ledger.md +0 -125
- package/skills/analytical-method-validation/references/statistics.md +0 -209
- package/skills/analytical-method-validation/scripts/_catalog.py +0 -499
- package/skills/analytical-method-validation/scripts/_common.py +0 -955
- package/skills/analytical-method-validation/scripts/check_accuracy_precision.py +0 -328
- package/skills/analytical-method-validation/scripts/check_bioanalytical_run.py +0 -293
- package/skills/analytical-method-validation/scripts/check_detection_limits.py +0 -310
- package/skills/analytical-method-validation/scripts/check_response.py +0 -243
- package/skills/analytical-method-validation/scripts/compare_methods.py +0 -215
- package/skills/analytical-method-validation/scripts/plan_validation.py +0 -381
- package/skills/anndata/references/best_practices.md +0 -532
- package/skills/anndata/references/concatenation.md +0 -399
- package/skills/anndata/references/data_structure.md +0 -314
- package/skills/anndata/references/io_operations.md +0 -466
- package/skills/anndata/references/manipulation.md +0 -516
- package/skills/arbor/references/arbor-upstream.md +0 -91
- package/skills/arbor/references/executor-brief.md +0 -68
- package/skills/arbor/references/htr-methodology.md +0 -177
- package/skills/arbor/references/report-template.md +0 -39
- package/skills/arbor/scripts/tree.py +0 -564
- package/skills/arboreto/references/algorithms.md +0 -152
- package/skills/arboreto/references/basic_inference.md +0 -181
- package/skills/arboreto/references/distributed_computing.md +0 -242
- package/skills/arboreto/scripts/basic_grn_inference.py +0 -107
- package/skills/astropy/references/coordinates.md +0 -280
- package/skills/astropy/references/cosmology.md +0 -309
- package/skills/astropy/references/fits.md +0 -398
- package/skills/astropy/references/tables.md +0 -495
- package/skills/astropy/references/time.md +0 -412
- package/skills/astropy/references/units.md +0 -178
- package/skills/astropy/references/wcs_and_other_modules.md +0 -377
- package/skills/autoskill/config.yaml +0 -53
- package/skills/autoskill/references/https-proxy.md +0 -62
- package/skills/autoskill/references/screenpipe-config.yaml +0 -61
- package/skills/autoskill/scripts/autoskill.py +0 -35
- package/skills/autoskill/scripts/backends.py +0 -116
- package/skills/autoskill/scripts/cluster.py +0 -54
- package/skills/autoskill/scripts/doctor.py +0 -108
- package/skills/autoskill/scripts/fetch_window.py +0 -33
- package/skills/autoskill/scripts/match_skills.py +0 -46
- package/skills/autoskill/scripts/promote.py +0 -58
- package/skills/autoskill/scripts/redact.py +0 -40
- package/skills/autoskill/scripts/run.py +0 -194
- package/skills/autoskill/scripts/synthesize.py +0 -72
- package/skills/benchling-integration/references/api_endpoints.md +0 -883
- package/skills/benchling-integration/references/authentication.md +0 -390
- package/skills/benchling-integration/references/core_capabilities.md +0 -355
- package/skills/benchling-integration/references/eventbridge.md +0 -255
- package/skills/benchling-integration/references/sdk_reference.md +0 -772
- package/skills/bids/references/beps.yml +0 -637
- package/skills/bids/references/bids_schema.json +0 -21015
- package/skills/bids/references/bids_specification.md +0 -165
- package/skills/bids/references/conversion_tools.md +0 -475
- package/skills/bids/references/core_workflows.md +0 -552
- package/skills/bids/references/metadata_fields.md +0 -365
- package/skills/bids/scripts/update_schema.py +0 -89
- package/skills/biopython/references/advanced.md +0 -580
- package/skills/biopython/references/alignment.md +0 -377
- package/skills/biopython/references/blast.md +0 -463
- package/skills/biopython/references/databases.md +0 -492
- package/skills/biopython/references/phylogenetics.md +0 -566
- package/skills/biopython/references/sequence_io.md +0 -289
- package/skills/biopython/references/structure.md +0 -564
- package/skills/bioservices/references/identifier_mapping.md +0 -685
- package/skills/bioservices/references/services_reference.md +0 -638
- package/skills/bioservices/references/workflow_patterns.md +0 -813
- package/skills/bioservices/scripts/batch_id_converter.py +0 -347
- package/skills/bioservices/scripts/compound_cross_reference.py +0 -387
- package/skills/bioservices/scripts/pathway_analysis.py +0 -309
- package/skills/bioservices/scripts/protein_analysis_workflow.py +0 -441
- package/skills/bulk-rnaseq/references/counts-and-handoff.md +0 -91
- package/skills/bulk-rnaseq/references/design-and-qc.md +0 -68
- package/skills/bulk-rnaseq/references/upstream-manual.md +0 -128
- package/skills/bulk-rnaseq/references/upstream-nfcore.md +0 -108
- package/skills/bulk-rnaseq/scripts/build_counts_matrix.py +0 -203
- package/skills/bulk-rnaseq/scripts/validate_samplesheet.py +0 -206
- package/skills/cellxgene-census/references/census_schema.md +0 -218
- package/skills/cellxgene-census/references/common_patterns.md +0 -368
- package/skills/cellxgene-census/references/core_workflow_patterns.md +0 -297
- package/skills/cirq/references/building.md +0 -307
- package/skills/cirq/references/experiments.md +0 -572
- package/skills/cirq/references/hardware.md +0 -527
- package/skills/cirq/references/noise.md +0 -514
- package/skills/cirq/references/simulation.md +0 -353
- package/skills/cirq/references/transformation.md +0 -416
- package/skills/citation-management/assets/bibtex_template.bib +0 -264
- package/skills/citation-management/assets/citation_checklist.md +0 -386
- package/skills/citation-management/references/best_practices.md +0 -91
- package/skills/citation-management/references/bibtex_formatting.md +0 -908
- package/skills/citation-management/references/citation_validation.md +0 -835
- package/skills/citation-management/references/core_workflow.md +0 -569
- package/skills/citation-management/references/example_workflows.md +0 -126
- package/skills/citation-management/references/google_scholar_search.md +0 -732
- package/skills/citation-management/references/metadata_extraction.md +0 -870
- package/skills/citation-management/references/pubmed_search.md +0 -839
- package/skills/citation-management/references/script_reference.md +0 -250
- package/skills/citation-management/references/search_strategies.md +0 -110
- package/skills/citation-management/scripts/_common.py +0 -331
- package/skills/citation-management/scripts/doi_to_bibtex.py +0 -204
- package/skills/citation-management/scripts/extract_metadata.py +0 -690
- package/skills/citation-management/scripts/format_bibtex.py +0 -356
- package/skills/citation-management/scripts/search_google_scholar.py +0 -268
- package/skills/citation-management/scripts/search_openalex.py +0 -297
- package/skills/citation-management/scripts/search_pubmed.py +0 -419
- package/skills/citation-management/scripts/validate_citations.py +0 -688
- package/skills/clinical-decision-support/assets/aggregate_cohort_table_template.json +0 -114
- package/skills/clinical-decision-support/assets/aggregate_model_evaluation_template.json +0 -115
- package/skills/clinical-decision-support/assets/artifact_intended_use_template.json +0 -81
- package/skills/clinical-decision-support/assets/decision_logic_traceability_template.json +0 -108
- package/skills/clinical-decision-support/assets/deidentification_checklist_template.json +0 -176
- package/skills/clinical-decision-support/assets/evidence_profile_template.json +0 -139
- package/skills/clinical-decision-support/assets/survival_analysis_plan_template.json +0 -134
- package/skills/clinical-decision-support/references/cohort_evaluation.md +0 -142
- package/skills/clinical-decision-support/references/decision_logic_traceability.md +0 -146
- package/skills/clinical-decision-support/references/evidence_profiles.md +0 -153
- package/skills/clinical-decision-support/references/model_biomarker_evaluation.md +0 -152
- package/skills/clinical-decision-support/references/privacy_and_disclosure.md +0 -144
- package/skills/clinical-decision-support/references/regulatory_and_governance.md +0 -104
- package/skills/clinical-decision-support/references/safety_and_scope.md +0 -103
- package/skills/clinical-decision-support/references/security_validation.md +0 -60
- package/skills/clinical-decision-support/references/sources.md +0 -119
- package/skills/clinical-decision-support/references/study_reporting.md +0 -134
- package/skills/clinical-decision-support/references/survival_analysis.md +0 -156
- package/skills/clinical-decision-support/scripts/_common.py +0 -223
- package/skills/clinical-decision-support/scripts/cohort_table_generator.py +0 -288
- package/skills/clinical-decision-support/scripts/decision_logic_traceability.py +0 -313
- package/skills/clinical-decision-support/scripts/deidentification_checklist.py +0 -260
- package/skills/clinical-decision-support/scripts/evidence_profile_check.py +0 -259
- package/skills/clinical-decision-support/scripts/model_biomarker_evaluation.py +0 -345
- package/skills/clinical-decision-support/scripts/survival_plan_validator.py +0 -294
- package/skills/clinical-decision-support/scripts/validate_cds_artifact.py +0 -269
- package/skills/clinical-reports/assets/adverse_event_aggregate_input_template.csv +0 -1
- package/skills/clinical-reports/assets/case_report_template.json +0 -43
- package/skills/clinical-reports/assets/clinical_trial_csr_template.json +0 -51
- package/skills/clinical-reports/assets/clinical_trial_results_template.json +0 -66
- package/skills/clinical-reports/assets/clinical_trial_safety_aggregate_template.json +0 -49
- package/skills/clinical-reports/assets/consistency_manifest_template.json +0 -15
- package/skills/clinical-reports/assets/deidentification_process_checklist.json +0 -56
- package/skills/clinical-reports/assets/lab_report_template.json +0 -36
- package/skills/clinical-reports/assets/pathology_report_template.json +0 -36
- package/skills/clinical-reports/assets/provenance_manifest_template.json +0 -17
- package/skills/clinical-reports/assets/quality_review_checklist.json +0 -34
- package/skills/clinical-reports/assets/radiology_report_template.json +0 -34
- package/skills/clinical-reports/assets/research_summary_template.json +0 -36
- package/skills/clinical-reports/assets/terminology_manifest_template.json +0 -11
- package/skills/clinical-reports/assets/trial_protocol_reporting_checklist.json +0 -70
- package/skills/clinical-reports/references/case_report_guidelines.md +0 -72
- package/skills/clinical-reports/references/clinical_trial_reporting.md +0 -97
- package/skills/clinical-reports/references/data_presentation.md +0 -92
- package/skills/clinical-reports/references/diagnostic_reports_standards.md +0 -76
- package/skills/clinical-reports/references/medical_terminology.md +0 -84
- package/skills/clinical-reports/references/privacy_and_deidentification.md +0 -84
- package/skills/clinical-reports/references/professional_review.md +0 -78
- package/skills/clinical-reports/references/report_type_routing.md +0 -57
- package/skills/clinical-reports/references/safety_reporting.md +0 -109
- package/skills/clinical-reports/references/sources.md +0 -75
- package/skills/clinical-reports/scripts/_common.py +0 -263
- package/skills/clinical-reports/scripts/check_deidentification.py +0 -298
- package/skills/clinical-reports/scripts/consistency_checker.py +0 -390
- package/skills/clinical-reports/scripts/format_adverse_events.py +0 -455
- package/skills/clinical-reports/scripts/generate_report_template.py +0 -115
- package/skills/clinical-reports/scripts/provenance_validator.py +0 -263
- package/skills/clinical-reports/scripts/terminology_validator.py +0 -277
- package/skills/clinical-reports/scripts/validate_case_report.py +0 -278
- package/skills/clinical-reports/scripts/validate_trial_report.py +0 -534
- package/skills/cobrapy/references/api_quick_reference.md +0 -665
- package/skills/cobrapy/references/workflows.md +0 -600
- package/skills/consciousness-council/references/advanced-configurations.md +0 -96
- package/skills/dask/references/arrays.md +0 -495
- package/skills/dask/references/bags.md +0 -468
- package/skills/dask/references/best-practices.md +0 -277
- package/skills/dask/references/dataframes.md +0 -370
- package/skills/dask/references/futures.md +0 -541
- package/skills/dask/references/schedulers.md +0 -517
- package/skills/database-lookup/references/addgene.md +0 -38
- package/skills/database-lookup/references/alphafold.md +0 -52
- package/skills/database-lookup/references/alphavantage.md +0 -261
- package/skills/database-lookup/references/bea.md +0 -409
- package/skills/database-lookup/references/bindingdb.md +0 -85
- package/skills/database-lookup/references/biogrid.md +0 -110
- package/skills/database-lookup/references/bls.md +0 -235
- package/skills/database-lookup/references/brenda.md +0 -71
- package/skills/database-lookup/references/cbioportal.md +0 -206
- package/skills/database-lookup/references/census.md +0 -251
- package/skills/database-lookup/references/chebi.md +0 -103
- package/skills/database-lookup/references/chembl.md +0 -80
- package/skills/database-lookup/references/clinicaltrials.md +0 -86
- package/skills/database-lookup/references/clinpgx.md +0 -64
- package/skills/database-lookup/references/clinvar.md +0 -91
- package/skills/database-lookup/references/cod.md +0 -121
- package/skills/database-lookup/references/cosmic.md +0 -59
- package/skills/database-lookup/references/dailymed.md +0 -65
- package/skills/database-lookup/references/database_selection_guide.md +0 -166
- package/skills/database-lookup/references/datacommons.md +0 -237
- package/skills/database-lookup/references/dbsnp.md +0 -143
- package/skills/database-lookup/references/disgenet.md +0 -52
- package/skills/database-lookup/references/drugbank.md +0 -54
- package/skills/database-lookup/references/ecb.md +0 -191
- package/skills/database-lookup/references/emdb.md +0 -37
- package/skills/database-lookup/references/ena.md +0 -372
- package/skills/database-lookup/references/encode.md +0 -47
- package/skills/database-lookup/references/ensembl.md +0 -539
- package/skills/database-lookup/references/epa.md +0 -232
- package/skills/database-lookup/references/eurostat.md +0 -237
- package/skills/database-lookup/references/fda.md +0 -64
- package/skills/database-lookup/references/federal-reserve.md +0 -216
- package/skills/database-lookup/references/fred.md +0 -297
- package/skills/database-lookup/references/gene-ontology.md +0 -147
- package/skills/database-lookup/references/geo.md +0 -130
- package/skills/database-lookup/references/gnomad.md +0 -93
- package/skills/database-lookup/references/gtex.md +0 -136
- package/skills/database-lookup/references/gwas-catalog.md +0 -46
- package/skills/database-lookup/references/hca.md +0 -35
- package/skills/database-lookup/references/hpo.md +0 -48
- package/skills/database-lookup/references/human-protein-atlas.md +0 -57
- package/skills/database-lookup/references/interpro.md +0 -120
- package/skills/database-lookup/references/jaspar.md +0 -50
- package/skills/database-lookup/references/kegg.md +0 -78
- package/skills/database-lookup/references/lincs-l1000.md +0 -68
- package/skills/database-lookup/references/materials-project.md +0 -123
- package/skills/database-lookup/references/metabolomics-workbench.md +0 -98
- package/skills/database-lookup/references/monarch.md +0 -46
- package/skills/database-lookup/references/mousemine.md +0 -40
- package/skills/database-lookup/references/nasa-exoplanet-archive.md +0 -112
- package/skills/database-lookup/references/nasa.md +0 -121
- package/skills/database-lookup/references/ncbi-gene.md +0 -64
- package/skills/database-lookup/references/ncbi-protein.md +0 -104
- package/skills/database-lookup/references/ncbi-taxonomy.md +0 -121
- package/skills/database-lookup/references/nist.md +0 -105
- package/skills/database-lookup/references/noaa.md +0 -199
- package/skills/database-lookup/references/omim.md +0 -114
- package/skills/database-lookup/references/opentargets.md +0 -459
- package/skills/database-lookup/references/openweathermap.md +0 -255
- package/skills/database-lookup/references/pdb.md +0 -121
- package/skills/database-lookup/references/pride.md +0 -74
- package/skills/database-lookup/references/pubchem.md +0 -145
- package/skills/database-lookup/references/quickgo.md +0 -45
- package/skills/database-lookup/references/reactome.md +0 -140
- package/skills/database-lookup/references/retrieval-contract.md +0 -123
- package/skills/database-lookup/references/rummageo.md +0 -32
- package/skills/database-lookup/references/sdss.md +0 -130
- package/skills/database-lookup/references/sec-edgar.md +0 -315
- package/skills/database-lookup/references/simbad.md +0 -405
- package/skills/database-lookup/references/sra.md +0 -149
- package/skills/database-lookup/references/string.md +0 -283
- package/skills/database-lookup/references/tcga-gdc.md +0 -58
- package/skills/database-lookup/references/treasury.md +0 -215
- package/skills/database-lookup/references/ucsc-genome.md +0 -135
- package/skills/database-lookup/references/uniprot.md +0 -283
- package/skills/database-lookup/references/usgs.md +0 -260
- package/skills/database-lookup/references/uspto.md +0 -130
- package/skills/database-lookup/references/who.md +0 -283
- package/skills/database-lookup/references/worldbank.md +0 -239
- package/skills/database-lookup/references/zinc.md +0 -202
- package/skills/datamol/references/conformers_module.md +0 -131
- package/skills/datamol/references/core_api.md +0 -136
- package/skills/datamol/references/core_workflows.md +0 -451
- package/skills/datamol/references/descriptors_viz.md +0 -195
- package/skills/datamol/references/fragments_scaffolds.md +0 -174
- package/skills/datamol/references/io_module.md +0 -112
- package/skills/datamol/references/reactions_data.md +0 -218
- package/skills/datamol/references/workflow_patterns.md +0 -104
- package/skills/deepchem/references/api_reference.md +0 -305
- package/skills/deepchem/references/core_capabilities.md +0 -276
- package/skills/deepchem/references/typical_workflows.md +0 -109
- package/skills/deepchem/references/workflows.md +0 -491
- package/skills/deepchem/scripts/graph_neural_network.py +0 -350
- package/skills/deepchem/scripts/predict_solubility.py +0 -223
- package/skills/deepchem/scripts/transfer_learning.py +0 -443
- package/skills/deepspot-m/references/api.md +0 -186
- package/skills/deepspot-m/references/whole_slide.md +0 -174
- package/skills/deeptools/assets/quick_reference.md +0 -65
- package/skills/deeptools/references/core_workflows.md +0 -134
- package/skills/deeptools/references/effective_genome_sizes.md +0 -118
- package/skills/deeptools/references/normalization_methods.md +0 -424
- package/skills/deeptools/references/tools_reference.md +0 -569
- package/skills/deeptools/references/workflows.md +0 -476
- package/skills/deeptools/scripts/validate_files.py +0 -195
- package/skills/deeptools/scripts/workflow_generator.py +0 -520
- package/skills/depmap/references/dependency_analysis.md +0 -178
- package/skills/dhdna-profiler/references/advanced-profiling.md +0 -72
- package/skills/diffdock/assets/batch_template.csv +0 -4
- package/skills/diffdock/assets/custom_inference_config.yaml +0 -94
- package/skills/diffdock/references/confidence_and_limitations.md +0 -182
- package/skills/diffdock/references/parameters_reference.md +0 -173
- package/skills/diffdock/references/workflows_examples.md +0 -401
- package/skills/diffdock/scripts/analyze_results.py +0 -346
- package/skills/diffdock/scripts/prepare_batch_csv.py +0 -257
- package/skills/diffdock/scripts/setup_check.py +0 -283
- package/skills/dnanexus-integration/references/app-development.md +0 -371
- package/skills/dnanexus-integration/references/authentication.md +0 -226
- package/skills/dnanexus-integration/references/configuration.md +0 -444
- package/skills/dnanexus-integration/references/data-operations.md +0 -474
- package/skills/dnanexus-integration/references/job-execution.md +0 -482
- package/skills/dnanexus-integration/references/operations-and-troubleshooting.md +0 -506
- package/skills/dnanexus-integration/references/python-sdk.md +0 -548
- package/skills/dnanexus-integration/references/sources.md +0 -168
- package/skills/dnanexus-integration/references/workflow-languages.md +0 -292
- package/skills/dnanexus-integration/scripts/inspect_dxpy.py +0 -366
- package/skills/dnanexus-integration/scripts/validate_dxapp.py +0 -796
- package/skills/docx/LICENSE.txt +0 -30
- package/skills/docx/scripts/__init__.py +0 -1
- package/skills/docx/scripts/accept_changes.py +0 -135
- package/skills/docx/scripts/comment.py +0 -368
- package/skills/docx/scripts/merge_runs.py +0 -310
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- package/skills/xlsx/scripts/office/schemas/microsoft/wml-cid-2016.xsd +0 -13
- package/skills/xlsx/scripts/office/schemas/microsoft/wml-sdtdatahash-2020.xsd +0 -4
- package/skills/xlsx/scripts/office/schemas/microsoft/wml-symex-2015.xsd +0 -8
- package/skills/xlsx/scripts/office/soffice.py +0 -232
- package/skills/xlsx/scripts/office/validate.py +0 -173
- package/skills/xlsx/scripts/office/validators/__init__.py +0 -15
- package/skills/xlsx/scripts/office/validators/base.py +0 -875
- package/skills/xlsx/scripts/office/validators/docx.py +0 -466
- package/skills/xlsx/scripts/office/validators/pptx.py +0 -441
- package/skills/xlsx/scripts/office/validators/redlining.py +0 -299
- package/skills/xlsx/scripts/recalc.py +0 -308
- package/skills/zarr-python/references/api_reference.md +0 -162
- package/skills/zarr-python/references/chunking_and_compression.md +0 -138
- package/skills/zarr-python/references/integration.md +0 -147
- package/skills/zarr-python/references/performance_and_patterns.md +0 -198
- package/skills/zarr-python/references/storage_backends.md +0 -91
- package/skills/zarr-python/references/v3_migration.md +0 -127
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# IQ-TREE 2 Phylogenetic Inference Reference
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## Basic Command Syntax
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```bash
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iqtree2 -s alignment.fasta --prefix output -m TEST -B 1000 -T AUTO --redo
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```
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## Key Parameters
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| Flag | Description | Default |
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| `-s` | Input alignment file | Required |
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| `--prefix` | Output file prefix | alignment name |
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| `-m` | Substitution model (or TEST) | GTR+G |
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| `-B` | Ultrafast bootstrap replicates | Off |
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| `-b` | Standard bootstrap replicates (slow) | Off |
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| `-T` | Number of threads (or AUTO) | 1 |
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| `-o` | Outgroup taxa name(s) | None (unrooted) |
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| `--redo` | Overwrite existing results | Off |
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| `-alrt` | SH-aLRT test replicates | Off |
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## Model Selection
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```bash
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# Full model testing (automatically selects best model)
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iqtree2 -s alignment.fasta -m TEST --prefix test_run -B 1000 -T 4
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# Specify model explicitly
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iqtree2 -s alignment.fasta -m GTR+G4 --prefix gtr_run -B 1000
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# Protein sequences
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iqtree2 -s protein.fasta -m TEST --prefix prot_tree -B 1000
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# Codon-based analysis
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iqtree2 -s codon.fasta -m GY --prefix codon_tree -B 1000
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```
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## Bootstrapping Methods
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### Ultrafast Bootstrap (UFBoot, recommended)
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```bash
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iqtree2 -s alignment.fasta -B 1000 # 1000 replicates
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# Values ≥95 are reliable
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# ~10× faster than standard bootstrap
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```
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### Standard Bootstrap
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```bash
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iqtree2 -s alignment.fasta -b 100 # 100 replicates (very slow)
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```
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### SH-aLRT Test (fast alternative)
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```bash
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iqtree2 -s alignment.fasta -alrt 1000 -B 1000 # Both SH-aLRT and UFBoot
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# SH-aLRT ≥80 AND UFBoot ≥95 = well-supported branch
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```
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## Branch Support Interpretation
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| Bootstrap Value | Interpretation |
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|----------------|----------------|
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| ≥ 95 | Well-supported (strongly supported) |
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| 70–94 | Moderately supported |
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| 50–69 | Weakly supported |
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| < 50 | Unreliable (not supported) |
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## Output Files
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| File | Description |
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|------|-------------|
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| `{prefix}.treefile` | Best ML tree in Newick format |
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| `{prefix}.iqtree` | Full analysis report |
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| `{prefix}.log` | Computation log |
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| `{prefix}.contree` | Consensus tree from bootstrap |
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| `{prefix}.splits.nex` | Network splits |
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| `{prefix}.bionj` | BioNJ starting tree |
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| `{prefix}.model.gz` | Saved model parameters |
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## Advanced Analyses
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### Molecular Clock (Dating)
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```bash
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# Temporal analysis with sampling dates
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iqtree2 -s alignment.fasta -m GTR+G \
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--date dates.tsv \ # Tab-separated: taxon_name YYYY-MM-DD
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--clock-test \ # Test for clock-like evolution
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--date-CI 95 \ # 95% CI for node dates
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--prefix dated_tree
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```
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### Concordance Factors
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```bash
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# Gene concordance factor (gCF) - requires multiple gene alignments
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iqtree2 --gcf gene_trees.nwk \
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--tree main_tree.treefile \
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--cf-verbose \
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--prefix cf_analysis
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```
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### Ancestral Sequence Reconstruction
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```bash
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iqtree2 -s alignment.fasta -m LG+G4 \
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-asr \ # Marginal ancestral state reconstruction
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--prefix anc_tree
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# Output: {prefix}.state (ancestral sequences per node)
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```
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### Partition Model (Multi-Gene)
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```bash
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# Create partition file (partitions.txt):
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# DNA, gene1 = 1-500
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# DNA, gene2 = 501-1000
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iqtree2 -s concat_alignment.fasta \
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-p partitions.txt \
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-m TEST \
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-B 1000 \
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--prefix partition_tree
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```
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## IQ-TREE Log Parsing
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```python
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def parse_iqtree_log(log_file: str) -> dict:
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"""Extract key results from IQ-TREE log file."""
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results = {}
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with open(log_file) as f:
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for line in f:
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if "Best-fit model" in line:
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results["best_model"] = line.split(":")[1].strip()
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elif "Log-likelihood of the tree:" in line:
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results["log_likelihood"] = float(line.split(":")[1].strip())
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elif "Number of free parameters" in line:
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results["free_params"] = int(line.split(":")[1].strip())
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elif "Akaike information criterion" in line:
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results["AIC"] = float(line.split(":")[1].strip())
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elif "Bayesian information criterion" in line:
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results["BIC"] = float(line.split(":")[1].strip())
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elif "Total CPU time used" in line:
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results["cpu_time"] = line.split(":")[1].strip()
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return results
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# Example:
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# results = parse_iqtree_log("output.log")
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# print(f"Best model: {results['best_model']}")
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# print(f"Log-likelihood: {results['log_likelihood']:.2f}")
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```
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## Common Issues and Solutions
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| Issue | Likely Cause | Solution |
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|-------|-------------|---------|
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| All bootstrap values = 0 | Too few taxa | Need ≥4 taxa for bootstrap |
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| Very long branches | Alignment artifacts | Re-trim alignment; check for outliers |
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| Memory error | Too many sequences | Use FastTree; or reduce `-T` to 1 |
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| Poor model fit | Wrong alphabet | Check nucleotide vs. protein specification |
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| Identical sequences | Duplicate sequences | Remove duplicates before alignment |
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## MAFFT Alignment Guide
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```bash
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# Accurate (< 200 sequences)
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mafft --localpair --maxiterate 1000 input.fasta > aligned.fasta
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# Medium (200-1000 sequences)
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mafft --auto input.fasta > aligned.fasta
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# Fast (> 1000 sequences)
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mafft --fftns input.fasta > aligned.fasta
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# Very large (> 10000 sequences)
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mafft --retree 1 input.fasta > aligned.fasta
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# Using multiple threads
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mafft --thread 8 --auto input.fasta > aligned.fasta
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```
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@@ -1,272 +0,0 @@
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"""
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Phylogenetic Analysis Pipeline
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===============================
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Complete workflow: MAFFT alignment → IQ-TREE tree → ETE3 visualization.
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Requirements:
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conda install -c bioconda mafft iqtree
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uv pip install ete3
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Usage:
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python phylogenetic_analysis.py sequences.fasta --type nt --threads 4
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python phylogenetic_analysis.py proteins.fasta --type aa --fasttree
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"""
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import argparse
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import os
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import subprocess
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import sys
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from pathlib import Path
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def check_dependencies():
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"""Check that required tools are installed."""
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tools = {
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"mafft": "conda install -c bioconda mafft",
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"iqtree2": "conda install -c bioconda iqtree",
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}
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missing = []
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for tool, install_cmd in tools.items():
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result = subprocess.run(["which", tool], capture_output=True)
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if result.returncode != 0:
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missing.append(f" {tool}: {install_cmd}")
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if missing:
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print("Missing dependencies:")
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for m in missing:
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print(m)
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sys.exit(1)
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print("All dependencies found.")
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42
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def count_sequences(fasta_file: str) -> int:
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"""Count sequences in a FASTA file."""
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with open(fasta_file) as f:
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return sum(1 for line in f if line.startswith('>'))
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-
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-
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def run_mafft(input_fasta: str, output_fasta: str, n_threads: int = 4,
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method: str = "auto") -> str:
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"""Run MAFFT multiple sequence alignment."""
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n_seqs = count_sequences(input_fasta)
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print(f"MAFFT: Aligning {n_seqs} sequences...")
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# Auto-select method based on dataset size
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if method == "auto":
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if n_seqs <= 200:
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cmd = ["mafft", "--localpair", "--maxiterate", "1000",
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"--thread", str(n_threads), "--inputorder", input_fasta]
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elif n_seqs <= 1000:
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cmd = ["mafft", "--auto", "--thread", str(n_threads),
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"--inputorder", input_fasta]
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else:
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cmd = ["mafft", "--fftns", "--thread", str(n_threads),
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"--inputorder", input_fasta]
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else:
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cmd = ["mafft", f"--{method}", "--thread", str(n_threads),
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"--inputorder", input_fasta]
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with open(output_fasta, 'w') as out:
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result = subprocess.run(cmd, stdout=out, stderr=subprocess.PIPE, text=True)
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if result.returncode != 0:
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raise RuntimeError(f"MAFFT failed:\n{result.stderr[:500]}")
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print(f" Alignment complete → {output_fasta}")
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return output_fasta
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def run_iqtree(aligned_fasta: str, prefix: str, seq_type: str = "nt",
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bootstrap: int = 1000, n_threads: int = 4,
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outgroup: str = None) -> str:
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"""Run IQ-TREE 2 phylogenetic inference."""
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print(f"IQ-TREE 2: Building maximum likelihood tree...")
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-
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cmd = [
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"iqtree2",
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"-s", aligned_fasta,
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"--prefix", prefix,
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"-m", "TEST", # Auto model selection
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"-B", str(bootstrap), # Ultrafast bootstrap
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"-T", str(n_threads),
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"--redo",
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"-alrt", "1000", # SH-aLRT test
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]
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if outgroup:
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cmd += ["-o", outgroup]
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result = subprocess.run(cmd, capture_output=True, text=True)
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if result.returncode != 0:
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raise RuntimeError(f"IQ-TREE failed:\n{result.stderr[:500]}")
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-
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tree_file = f"{prefix}.treefile"
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105
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106
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# Extract best model from log
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log_file = f"{prefix}.log"
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if os.path.exists(log_file):
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with open(log_file) as f:
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for line in f:
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if "Best-fit model" in line:
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print(f" {line.strip()}")
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print(f" Tree saved → {tree_file}")
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return tree_file
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-
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def run_fasttree(aligned_fasta: str, output_tree: str, seq_type: str = "nt") -> str:
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"""Run FastTree (faster alternative for large datasets)."""
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print("FastTree: Building approximate ML tree (faster)...")
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-
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if seq_type == "nt":
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cmd = ["FastTree", "-nt", "-gtr", "-gamma", aligned_fasta]
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else:
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cmd = ["FastTree", "-lg", "-gamma", aligned_fasta]
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-
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with open(output_tree, 'w') as out:
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result = subprocess.run(cmd, stdout=out, stderr=subprocess.PIPE, text=True)
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129
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|
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if result.returncode != 0:
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raise RuntimeError(f"FastTree failed:\n{result.stderr[:500]}")
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print(f" Tree saved → {output_tree}")
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return output_tree
|
|
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|
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|
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|
-
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137
|
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def visualize_tree(tree_file: str, output_png: str, outgroup: str = None) -> None:
|
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138
|
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"""Visualize the phylogenetic tree with ETE3."""
|
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139
|
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try:
|
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140
|
-
from ete3 import Tree, TreeStyle, NodeStyle
|
|
141
|
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except ImportError as exc:
|
|
142
|
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# TreeStyle and NodeStyle live in ete3's Qt-backed treeview module, so
|
|
143
|
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# this also fires when ete3 itself imported fine but PyQt5 is missing.
|
|
144
|
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print(f"ETE3 rendering unavailable ({exc}). Skipping visualization.")
|
|
145
|
-
print(" Install: uv pip install ete3 PyQt5")
|
|
146
|
-
return
|
|
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|
-
|
|
148
|
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t = Tree(tree_file)
|
|
149
|
-
|
|
150
|
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# Root the tree
|
|
151
|
-
if outgroup and outgroup in [leaf.name for leaf in t.get_leaves()]:
|
|
152
|
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t.set_outgroup(outgroup)
|
|
153
|
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print(f" Rooted at outgroup: {outgroup}")
|
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154
|
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else:
|
|
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|
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# Midpoint rooting
|
|
156
|
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t.set_outgroup(t.get_midpoint_outgroup())
|
|
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|
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print(" Applied midpoint rooting")
|
|
158
|
-
|
|
159
|
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# Style
|
|
160
|
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ts = TreeStyle()
|
|
161
|
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ts.show_leaf_name = True
|
|
162
|
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ts.show_branch_support = True
|
|
163
|
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ts.mode = "r" # rectangular
|
|
164
|
-
|
|
165
|
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try:
|
|
166
|
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t.render(output_png, tree_style=ts, w=800, units="px")
|
|
167
|
-
print(f" Visualization saved → {output_png}")
|
|
168
|
-
except Exception as e:
|
|
169
|
-
print(f" Visualization failed (display issue?): {e}")
|
|
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|
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# Save tree in Newick format as fallback
|
|
171
|
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rooted_nwk = output_png.replace(".png", "_rooted.nwk")
|
|
172
|
-
t.write(format=1, outfile=rooted_nwk)
|
|
173
|
-
print(f" Rooted tree saved → {rooted_nwk}")
|
|
174
|
-
|
|
175
|
-
|
|
176
|
-
def tree_summary(tree_file: str) -> dict:
|
|
177
|
-
"""Print summary statistics for the tree."""
|
|
178
|
-
try:
|
|
179
|
-
from ete3 import Tree
|
|
180
|
-
t = Tree(tree_file)
|
|
181
|
-
t.set_outgroup(t.get_midpoint_outgroup())
|
|
182
|
-
|
|
183
|
-
leaves = t.get_leaves()
|
|
184
|
-
branch_lengths = [n.dist for n in t.traverse() if n.dist > 0]
|
|
185
|
-
|
|
186
|
-
stats = {
|
|
187
|
-
"n_taxa": len(leaves),
|
|
188
|
-
"total_branch_length": sum(branch_lengths),
|
|
189
|
-
"mean_branch_length": sum(branch_lengths) / len(branch_lengths) if branch_lengths else 0,
|
|
190
|
-
"max_branch_length": max(branch_lengths) if branch_lengths else 0,
|
|
191
|
-
}
|
|
192
|
-
|
|
193
|
-
print("\nTree Summary:")
|
|
194
|
-
for k, v in stats.items():
|
|
195
|
-
if isinstance(v, float):
|
|
196
|
-
print(f" {k}: {v:.6f}")
|
|
197
|
-
else:
|
|
198
|
-
print(f" {k}: {v}")
|
|
199
|
-
|
|
200
|
-
return stats
|
|
201
|
-
except Exception as e:
|
|
202
|
-
print(f"Could not compute tree stats: {e}")
|
|
203
|
-
return {}
|
|
204
|
-
|
|
205
|
-
|
|
206
|
-
def main():
|
|
207
|
-
parser = argparse.ArgumentParser(description="Phylogenetic analysis pipeline")
|
|
208
|
-
parser.add_argument("input", help="Input FASTA file (unaligned)")
|
|
209
|
-
parser.add_argument("--type", choices=["nt", "aa"], default="nt",
|
|
210
|
-
help="Sequence type: nt (nucleotide) or aa (amino acid)")
|
|
211
|
-
parser.add_argument("--threads", type=int, default=4, help="Number of threads")
|
|
212
|
-
parser.add_argument("--bootstrap", type=int, default=1000,
|
|
213
|
-
help="Bootstrap replicates for IQ-TREE")
|
|
214
|
-
parser.add_argument("--fasttree", action="store_true",
|
|
215
|
-
help="Use FastTree instead of IQ-TREE (faster, less accurate)")
|
|
216
|
-
parser.add_argument("--outgroup", help="Outgroup taxon name for rooting")
|
|
217
|
-
parser.add_argument("--mafft-method", default="auto",
|
|
218
|
-
choices=["auto", "linsi", "einsi", "fftnsi", "fftns"],
|
|
219
|
-
help="MAFFT alignment method")
|
|
220
|
-
parser.add_argument("--output-dir", default="phylo_results",
|
|
221
|
-
help="Output directory")
|
|
222
|
-
|
|
223
|
-
args = parser.parse_args()
|
|
224
|
-
|
|
225
|
-
# Setup
|
|
226
|
-
os.makedirs(args.output_dir, exist_ok=True)
|
|
227
|
-
prefix = os.path.join(args.output_dir, Path(args.input).stem)
|
|
228
|
-
|
|
229
|
-
print("=" * 60)
|
|
230
|
-
print("Phylogenetic Analysis Pipeline")
|
|
231
|
-
print("=" * 60)
|
|
232
|
-
print(f"Input: {args.input}")
|
|
233
|
-
print(f"Sequence type: {args.type}")
|
|
234
|
-
print(f"Output dir: {args.output_dir}")
|
|
235
|
-
|
|
236
|
-
# Step 1: Multiple Sequence Alignment
|
|
237
|
-
print("\n[Step 1/3] Multiple Sequence Alignment (MAFFT)")
|
|
238
|
-
aligned = run_mafft(
|
|
239
|
-
args.input,
|
|
240
|
-
f"{prefix}_aligned.fasta",
|
|
241
|
-
n_threads=args.threads,
|
|
242
|
-
method=args.mafft_method
|
|
243
|
-
)
|
|
244
|
-
|
|
245
|
-
# Step 2: Tree Inference
|
|
246
|
-
print("\n[Step 2/3] Tree Inference")
|
|
247
|
-
if args.fasttree:
|
|
248
|
-
tree_file = run_fasttree(aligned, f"{prefix}.tree", seq_type=args.type)
|
|
249
|
-
else:
|
|
250
|
-
tree_file = run_iqtree(
|
|
251
|
-
aligned, prefix,
|
|
252
|
-
seq_type=args.type,
|
|
253
|
-
bootstrap=args.bootstrap,
|
|
254
|
-
n_threads=args.threads,
|
|
255
|
-
outgroup=args.outgroup
|
|
256
|
-
)
|
|
257
|
-
|
|
258
|
-
# Step 3: Visualization
|
|
259
|
-
print("\n[Step 3/3] Visualization (ETE3)")
|
|
260
|
-
visualize_tree(tree_file, f"{prefix}_tree.png", outgroup=args.outgroup)
|
|
261
|
-
tree_summary(tree_file)
|
|
262
|
-
|
|
263
|
-
print("\n" + "=" * 60)
|
|
264
|
-
print("Analysis complete!")
|
|
265
|
-
print(f"Key outputs:")
|
|
266
|
-
print(f" Aligned sequences: {aligned}")
|
|
267
|
-
print(f" Tree file: {tree_file}")
|
|
268
|
-
print(f" Visualization: {prefix}_tree.png")
|
|
269
|
-
|
|
270
|
-
|
|
271
|
-
if __name__ == "__main__":
|
|
272
|
-
main()
|
|
@@ -1,76 +0,0 @@
|
|
|
1
|
-
# Compaction and Branch Summarization
|
|
2
|
-
|
|
3
|
-
Source: https://pi.dev/docs/latest/compaction
|
|
4
|
-
|
|
5
|
-
Pi has two summarization mechanisms that share the same structured summary format and track file operations cumulatively.
|
|
6
|
-
|
|
7
|
-
| Mechanism | Trigger | Purpose |
|
|
8
|
-
|---|---|---|
|
|
9
|
-
| Compaction | context exceeds threshold, or `/compact` | Summarize old messages to free context |
|
|
10
|
-
| Branch summarization | `/tree` navigation | Preserve context when switching branches |
|
|
11
|
-
|
|
12
|
-
Both use fresh routing session IDs and, where the provider supports it, disable prompt-cache writes because these one-off prompts are unlikely to be reused.
|
|
13
|
-
|
|
14
|
-
## Auto-Compaction
|
|
15
|
-
|
|
16
|
-
Triggers when `contextTokens > contextWindow - reserveTokens`. Defaults: `reserveTokens` 16384, `keepRecentTokens` 20000, configured under `compaction` in global or project settings. `/compact [instructions]` works even with auto-compaction disabled.
|
|
17
|
-
|
|
18
|
-
Steps: walk backwards from the newest message accumulating token estimates until `keepRecentTokens` is reached (the cut point) → collect messages from the previous kept boundary (or session start) to the cut point → summarize with the structured format, passing any previous summary as iterative context → append a `CompactionEntry` → rebuild the context for the next request as summary plus messages from `firstKeptEntryId`.
|
|
19
|
-
|
|
20
|
-
On repeated compactions the summarized span starts at the previous compaction's kept boundary (`firstKeptEntryId`), not at the compaction entry, falling back to the entry after the previous compaction when that kept entry is not on the path. This re-includes messages that survived the earlier pass. `tokensBefore` is recalculated from the rebuilt context before writing the new entry.
|
|
21
|
-
|
|
22
|
-
Valid cut points: user messages, assistant messages, bash execution messages, and custom messages (`custom_message`, `branch_summary`). Pi never cuts at tool results.
|
|
23
|
-
|
|
24
|
-
## Split Turns
|
|
25
|
-
|
|
26
|
-
A turn starts with a user message and includes all assistant responses and tool calls until the next user message. When a single turn exceeds `keepRecentTokens`, the cut lands mid-turn at an assistant message (`isSplitTurn`). Pi then generates two summaries — a history summary for previous context and a turn-prefix summary for the early part of the split turn — and merges them.
|
|
27
|
-
|
|
28
|
-
## Branch Summarization
|
|
29
|
-
|
|
30
|
-
On `/tree` navigation to a different branch: find the deepest common ancestor, walk from the old leaf back to it, include messages up to the token budget newest-first, summarize, and append a `BranchSummaryEntry` at the navigation point — the summary lands on the destination branch's new leaf, not on the branch being left.
|
|
31
|
-
|
|
32
|
-
Both mechanisms extract file operations from the tool calls being summarized **and** from previous compaction/branch-summary `details`, so read/modified file tracking accumulates across passes.
|
|
33
|
-
|
|
34
|
-
## Entry Shapes
|
|
35
|
-
|
|
36
|
-
`CompactionEntry`: `type`, `id`, `parentId`, `timestamp`, `summary`, `firstKeptEntryId`, `tokensBefore`, optional `usage` (LLM usage that generated the summary; counted in session totals), optional `fromHook` (legacy name for "provided by extension"), optional `details`.
|
|
37
|
-
|
|
38
|
-
`BranchSummaryEntry`: same plus `fromId` instead of `firstKeptEntryId`.
|
|
39
|
-
|
|
40
|
-
Default `details` is `{ readFiles: string[], modifiedFiles: string[] }`; extensions may store any JSON-serializable structure. Newer harness-generated compactions also embed `retainedTail` — see `references/session-format.md`.
|
|
41
|
-
|
|
42
|
-
## Summary Format
|
|
43
|
-
|
|
44
|
-
Sections: `## Goal`, `## Constraints & Preferences`, `## Progress` (Done / In Progress / Blocked), `## Key Decisions`, `## Next Steps`, `## Critical Context`, then `<read-files>` and `<modified-files>` blocks.
|
|
45
|
-
|
|
46
|
-
## Message Serialization
|
|
47
|
-
|
|
48
|
-
`serializeConversation()` renders messages as `[User]:`, `[Assistant thinking]:`, `[Assistant]:`, `[Assistant tool calls]:`, `[Tool result]:` lines so the model does not treat the input as a conversation to continue. Tool results are truncated to 2000 characters during serialization, with a marker showing how many characters were dropped — `read` and `bash` results are usually the largest contributors to context.
|
|
49
|
-
|
|
50
|
-
## Extension Hooks
|
|
51
|
-
|
|
52
|
-
`session_before_compact` receives `{ preparation, branchEntries, customInstructions, reason, willRetry, signal }`. `preparation` exposes `messagesToSummarize`, `turnPrefixMessages`, `previousSummary`, `fileOps`, `tokensBefore`, `firstKeptEntryId`, and `settings`; `reason` is `"manual"`, `"threshold"`, or `"overflow"`; `willRetry` indicates overflow recovery. Return `{ cancel: true }` or `{ compaction: { summary, firstKeptEntryId, tokensBefore, usage?, details? } }`.
|
|
53
|
-
|
|
54
|
-
To summarize with your own model, convert messages first:
|
|
55
|
-
|
|
56
|
-
```ts
|
|
57
|
-
import { convertToLlm, serializeConversation } from "@earendil-works/pi-coding-agent";
|
|
58
|
-
|
|
59
|
-
const text = serializeConversation(convertToLlm(preparation.messagesToSummarize));
|
|
60
|
-
```
|
|
61
|
-
|
|
62
|
-
`session_before_tree` receives `{ preparation, signal }` with `targetId`, `oldLeafId`, `commonAncestorId`, `entriesToSummarize`, and `userWantsSummary`. It always fires, whether or not the user chose to summarize. Return `{ cancel: true }` to cancel navigation, or `{ summary: { summary, usage?, details? } }` (used only when `userWantsSummary`).
|
|
63
|
-
|
|
64
|
-
For direct programmatic summarization, `generateSummary()` returns the text and `generateSummaryWithUsage()` returns `{ text, usage }`.
|
|
65
|
-
|
|
66
|
-
## Settings
|
|
67
|
-
|
|
68
|
-
```json
|
|
69
|
-
{
|
|
70
|
-
"compaction": {
|
|
71
|
-
"enabled": true,
|
|
72
|
-
"reserveTokens": 16384,
|
|
73
|
-
"keepRecentTokens": 20000
|
|
74
|
-
}
|
|
75
|
-
}
|
|
76
|
-
```
|
|
@@ -1,80 +0,0 @@
|
|
|
1
|
-
# Containerization
|
|
2
|
-
|
|
3
|
-
Source: https://pi.dev/docs/latest/containerization
|
|
4
|
-
|
|
5
|
-
Pi runs with all permissions by default. Two general approaches: run the whole `pi` process inside an isolated environment, or run `pi` on the host and route tool execution into an isolated environment.
|
|
6
|
-
|
|
7
|
-
| Pattern | What is isolated | Best for | Notes |
|
|
8
|
-
|---|---|---|---|
|
|
9
|
-
| Gondolin extension | Built-in tools and `!` commands | Local micro-VM isolation while keeping auth on host | `examples/extensions/gondolin/` |
|
|
10
|
-
| Plain Docker | Whole `pi` process | Simplest local container boundary | Provider API keys enter the container |
|
|
11
|
-
| OpenShell | Whole `pi` process | Local or remote policy-controlled sandbox | Requires an OpenShell gateway |
|
|
12
|
-
|
|
13
|
-
Extensions run wherever the `pi` process runs. If host Pi routes built-ins into a VM, other custom extension tools still run on the host unless they delegate too.
|
|
14
|
-
|
|
15
|
-
## Gondolin
|
|
16
|
-
|
|
17
|
-
[Gondolin](https://github.com/earendil-works/gondolin) is a local Linux micro-VM.
|
|
18
|
-
|
|
19
|
-
```bash
|
|
20
|
-
cp -R packages/coding-agent/examples/extensions/gondolin ~/.pi/agent/extensions/gondolin
|
|
21
|
-
cd ~/.pi/agent/extensions/gondolin
|
|
22
|
-
npm install --ignore-scripts
|
|
23
|
-
|
|
24
|
-
cd /path/to/project
|
|
25
|
-
pi -e ~/.pi/agent/extensions/gondolin
|
|
26
|
-
```
|
|
27
|
-
|
|
28
|
-
The extension mounts the host cwd at `/workspace` in the VM and overrides `read`, `write`, `edit`, `bash`, `grep`, `find`, and `ls`. User `!` commands are routed into the VM as well, and file changes under `/workspace` write through to the host.
|
|
29
|
-
|
|
30
|
-
Requirements: Node.js >= 23.6.0 for `@earendil-works/gondolin`, plus QEMU installed through your package manager.
|
|
31
|
-
|
|
32
|
-
## Plain Docker
|
|
33
|
-
|
|
34
|
-
`Dockerfile.pi`:
|
|
35
|
-
|
|
36
|
-
```dockerfile
|
|
37
|
-
FROM node:24-bookworm-slim
|
|
38
|
-
|
|
39
|
-
RUN apt-get update \
|
|
40
|
-
&& apt-get install -y --no-install-recommends bash ca-certificates git ripgrep \
|
|
41
|
-
&& rm -rf /var/lib/apt/lists/*
|
|
42
|
-
RUN npm install -g --ignore-scripts @earendil-works/pi-coding-agent
|
|
43
|
-
|
|
44
|
-
WORKDIR /workspace
|
|
45
|
-
ENTRYPOINT ["pi"]
|
|
46
|
-
```
|
|
47
|
-
|
|
48
|
-
```bash
|
|
49
|
-
docker build -t pi-sandbox -f Dockerfile.pi .
|
|
50
|
-
|
|
51
|
-
docker run --rm -it \
|
|
52
|
-
-e ANTHROPIC_API_KEY \
|
|
53
|
-
-v "$PWD:/workspace" \
|
|
54
|
-
-v pi-agent-home:/root/.pi/agent \
|
|
55
|
-
pi-sandbox
|
|
56
|
-
```
|
|
57
|
-
|
|
58
|
-
`-v "$PWD:/workspace"` means reads and writes inside `/workspace` affect host files directly. Use a named volume for `/root/.pi/agent` if you want container-local settings and sessions — mounting host `~/.pi/agent` exposes host auth and session files to the container.
|
|
59
|
-
|
|
60
|
-
## OpenShell
|
|
61
|
-
|
|
62
|
-
[NVIDIA OpenShell](https://docs.nvidia.com/openshell/about/overview) provides a policy-controlled sandbox with filesystem, process, network, credential, and inference controls. It runs sandboxes through a local gateway backed by Docker, Podman, or a VM runtime, or through a remote Kubernetes gateway. Every sandbox requires an active gateway:
|
|
63
|
-
|
|
64
|
-
```bash
|
|
65
|
-
openshell gateway add <gateway-url> --name <name>
|
|
66
|
-
openshell gateway select <name>
|
|
67
|
-
|
|
68
|
-
openshell sandbox create --name pi-sandbox --from pi -- pi
|
|
69
|
-
```
|
|
70
|
-
|
|
71
|
-
The whole `pi` process runs inside the sandbox, so built-in tools, `!` commands, and extension tools all execute inside the boundary.
|
|
72
|
-
|
|
73
|
-
Remote gateways do not bind-mount host project files, so sandbox writes are not reflected on your machine. Clone the repository inside the sandbox or transfer files explicitly:
|
|
74
|
-
|
|
75
|
-
```bash
|
|
76
|
-
openshell sandbox upload pi-sandbox ./repo /workspace
|
|
77
|
-
openshell sandbox download pi-sandbox /workspace/repo ./repo-out
|
|
78
|
-
```
|
|
79
|
-
|
|
80
|
-
OpenShell providers can keep raw model API keys outside the sandbox: with inference routing configured, code inside the sandbox calls `https://inference.local` and the gateway injects provider credentials upstream. Point Pi at the corresponding OpenAI-compatible or Anthropic-compatible endpoint to use that route.
|