@pikaa-ai/pikaa 0.3.1 → 0.3.4
This diff represents the content of publicly available package versions that have been released to one of the supported registries. The information contained in this diff is provided for informational purposes only and reflects changes between package versions as they appear in their respective public registries.
- package/dist/cli.js +1169 -601
- package/dist/index.js +525 -204
- package/package.json +5 -3
- package/skills/adaptyv/references/api-endpoints.md +0 -690
- package/skills/aeon/references/anomaly_detection.md +0 -154
- package/skills/aeon/references/classification.md +0 -144
- package/skills/aeon/references/clustering.md +0 -123
- package/skills/aeon/references/datasets_benchmarking.md +0 -392
- package/skills/aeon/references/distances.md +0 -256
- package/skills/aeon/references/forecasting.md +0 -109
- package/skills/aeon/references/networks.md +0 -289
- package/skills/aeon/references/regression.md +0 -118
- package/skills/aeon/references/segmentation.md +0 -163
- package/skills/aeon/references/similarity_search.md +0 -187
- package/skills/aeon/references/transformations.md +0 -246
- package/skills/analytical-method-validation/assets/validation-protocol-template.md +0 -106
- package/skills/analytical-method-validation/assets/validation-report-template.md +0 -116
- package/skills/analytical-method-validation/references/compendial-and-clsi.md +0 -96
- package/skills/analytical-method-validation/references/framework-selection.md +0 -87
- package/skills/analytical-method-validation/references/ich-m10-bioanalytical.md +0 -123
- package/skills/analytical-method-validation/references/ich-q2r2.md +0 -229
- package/skills/analytical-method-validation/references/source-ledger.md +0 -125
- package/skills/analytical-method-validation/references/statistics.md +0 -209
- package/skills/analytical-method-validation/scripts/_catalog.py +0 -499
- package/skills/analytical-method-validation/scripts/_common.py +0 -955
- package/skills/analytical-method-validation/scripts/check_accuracy_precision.py +0 -328
- package/skills/analytical-method-validation/scripts/check_bioanalytical_run.py +0 -293
- package/skills/analytical-method-validation/scripts/check_detection_limits.py +0 -310
- package/skills/analytical-method-validation/scripts/check_response.py +0 -243
- package/skills/analytical-method-validation/scripts/compare_methods.py +0 -215
- package/skills/analytical-method-validation/scripts/plan_validation.py +0 -381
- package/skills/anndata/references/best_practices.md +0 -532
- package/skills/anndata/references/concatenation.md +0 -399
- package/skills/anndata/references/data_structure.md +0 -314
- package/skills/anndata/references/io_operations.md +0 -466
- package/skills/anndata/references/manipulation.md +0 -516
- package/skills/arbor/references/arbor-upstream.md +0 -91
- package/skills/arbor/references/executor-brief.md +0 -68
- package/skills/arbor/references/htr-methodology.md +0 -177
- package/skills/arbor/references/report-template.md +0 -39
- package/skills/arbor/scripts/tree.py +0 -564
- package/skills/arboreto/references/algorithms.md +0 -152
- package/skills/arboreto/references/basic_inference.md +0 -181
- package/skills/arboreto/references/distributed_computing.md +0 -242
- package/skills/arboreto/scripts/basic_grn_inference.py +0 -107
- package/skills/astropy/references/coordinates.md +0 -280
- package/skills/astropy/references/cosmology.md +0 -309
- package/skills/astropy/references/fits.md +0 -398
- package/skills/astropy/references/tables.md +0 -495
- package/skills/astropy/references/time.md +0 -412
- package/skills/astropy/references/units.md +0 -178
- package/skills/astropy/references/wcs_and_other_modules.md +0 -377
- package/skills/autoskill/config.yaml +0 -53
- package/skills/autoskill/references/https-proxy.md +0 -62
- package/skills/autoskill/references/screenpipe-config.yaml +0 -61
- package/skills/autoskill/scripts/autoskill.py +0 -35
- package/skills/autoskill/scripts/backends.py +0 -116
- package/skills/autoskill/scripts/cluster.py +0 -54
- package/skills/autoskill/scripts/doctor.py +0 -108
- package/skills/autoskill/scripts/fetch_window.py +0 -33
- package/skills/autoskill/scripts/match_skills.py +0 -46
- package/skills/autoskill/scripts/promote.py +0 -58
- package/skills/autoskill/scripts/redact.py +0 -40
- package/skills/autoskill/scripts/run.py +0 -194
- package/skills/autoskill/scripts/synthesize.py +0 -72
- package/skills/benchling-integration/references/api_endpoints.md +0 -883
- package/skills/benchling-integration/references/authentication.md +0 -390
- package/skills/benchling-integration/references/core_capabilities.md +0 -355
- package/skills/benchling-integration/references/eventbridge.md +0 -255
- package/skills/benchling-integration/references/sdk_reference.md +0 -772
- package/skills/bids/references/beps.yml +0 -637
- package/skills/bids/references/bids_schema.json +0 -21015
- package/skills/bids/references/bids_specification.md +0 -165
- package/skills/bids/references/conversion_tools.md +0 -475
- package/skills/bids/references/core_workflows.md +0 -552
- package/skills/bids/references/metadata_fields.md +0 -365
- package/skills/bids/scripts/update_schema.py +0 -89
- package/skills/biopython/references/advanced.md +0 -580
- package/skills/biopython/references/alignment.md +0 -377
- package/skills/biopython/references/blast.md +0 -463
- package/skills/biopython/references/databases.md +0 -492
- package/skills/biopython/references/phylogenetics.md +0 -566
- package/skills/biopython/references/sequence_io.md +0 -289
- package/skills/biopython/references/structure.md +0 -564
- package/skills/bioservices/references/identifier_mapping.md +0 -685
- package/skills/bioservices/references/services_reference.md +0 -638
- package/skills/bioservices/references/workflow_patterns.md +0 -813
- package/skills/bioservices/scripts/batch_id_converter.py +0 -347
- package/skills/bioservices/scripts/compound_cross_reference.py +0 -387
- package/skills/bioservices/scripts/pathway_analysis.py +0 -309
- package/skills/bioservices/scripts/protein_analysis_workflow.py +0 -441
- package/skills/bulk-rnaseq/references/counts-and-handoff.md +0 -91
- package/skills/bulk-rnaseq/references/design-and-qc.md +0 -68
- package/skills/bulk-rnaseq/references/upstream-manual.md +0 -128
- package/skills/bulk-rnaseq/references/upstream-nfcore.md +0 -108
- package/skills/bulk-rnaseq/scripts/build_counts_matrix.py +0 -203
- package/skills/bulk-rnaseq/scripts/validate_samplesheet.py +0 -206
- package/skills/cellxgene-census/references/census_schema.md +0 -218
- package/skills/cellxgene-census/references/common_patterns.md +0 -368
- package/skills/cellxgene-census/references/core_workflow_patterns.md +0 -297
- package/skills/cirq/references/building.md +0 -307
- package/skills/cirq/references/experiments.md +0 -572
- package/skills/cirq/references/hardware.md +0 -527
- package/skills/cirq/references/noise.md +0 -514
- package/skills/cirq/references/simulation.md +0 -353
- package/skills/cirq/references/transformation.md +0 -416
- package/skills/citation-management/assets/bibtex_template.bib +0 -264
- package/skills/citation-management/assets/citation_checklist.md +0 -386
- package/skills/citation-management/references/best_practices.md +0 -91
- package/skills/citation-management/references/bibtex_formatting.md +0 -908
- package/skills/citation-management/references/citation_validation.md +0 -835
- package/skills/citation-management/references/core_workflow.md +0 -569
- package/skills/citation-management/references/example_workflows.md +0 -126
- package/skills/citation-management/references/google_scholar_search.md +0 -732
- package/skills/citation-management/references/metadata_extraction.md +0 -870
- package/skills/citation-management/references/pubmed_search.md +0 -839
- package/skills/citation-management/references/script_reference.md +0 -250
- package/skills/citation-management/references/search_strategies.md +0 -110
- package/skills/citation-management/scripts/_common.py +0 -331
- package/skills/citation-management/scripts/doi_to_bibtex.py +0 -204
- package/skills/citation-management/scripts/extract_metadata.py +0 -690
- package/skills/citation-management/scripts/format_bibtex.py +0 -356
- package/skills/citation-management/scripts/search_google_scholar.py +0 -268
- package/skills/citation-management/scripts/search_openalex.py +0 -297
- package/skills/citation-management/scripts/search_pubmed.py +0 -419
- package/skills/citation-management/scripts/validate_citations.py +0 -688
- package/skills/clinical-decision-support/assets/aggregate_cohort_table_template.json +0 -114
- package/skills/clinical-decision-support/assets/aggregate_model_evaluation_template.json +0 -115
- package/skills/clinical-decision-support/assets/artifact_intended_use_template.json +0 -81
- package/skills/clinical-decision-support/assets/decision_logic_traceability_template.json +0 -108
- package/skills/clinical-decision-support/assets/deidentification_checklist_template.json +0 -176
- package/skills/clinical-decision-support/assets/evidence_profile_template.json +0 -139
- package/skills/clinical-decision-support/assets/survival_analysis_plan_template.json +0 -134
- package/skills/clinical-decision-support/references/cohort_evaluation.md +0 -142
- package/skills/clinical-decision-support/references/decision_logic_traceability.md +0 -146
- package/skills/clinical-decision-support/references/evidence_profiles.md +0 -153
- package/skills/clinical-decision-support/references/model_biomarker_evaluation.md +0 -152
- package/skills/clinical-decision-support/references/privacy_and_disclosure.md +0 -144
- package/skills/clinical-decision-support/references/regulatory_and_governance.md +0 -104
- package/skills/clinical-decision-support/references/safety_and_scope.md +0 -103
- package/skills/clinical-decision-support/references/security_validation.md +0 -60
- package/skills/clinical-decision-support/references/sources.md +0 -119
- package/skills/clinical-decision-support/references/study_reporting.md +0 -134
- package/skills/clinical-decision-support/references/survival_analysis.md +0 -156
- package/skills/clinical-decision-support/scripts/_common.py +0 -223
- package/skills/clinical-decision-support/scripts/cohort_table_generator.py +0 -288
- package/skills/clinical-decision-support/scripts/decision_logic_traceability.py +0 -313
- package/skills/clinical-decision-support/scripts/deidentification_checklist.py +0 -260
- package/skills/clinical-decision-support/scripts/evidence_profile_check.py +0 -259
- package/skills/clinical-decision-support/scripts/model_biomarker_evaluation.py +0 -345
- package/skills/clinical-decision-support/scripts/survival_plan_validator.py +0 -294
- package/skills/clinical-decision-support/scripts/validate_cds_artifact.py +0 -269
- package/skills/clinical-reports/assets/adverse_event_aggregate_input_template.csv +0 -1
- package/skills/clinical-reports/assets/case_report_template.json +0 -43
- package/skills/clinical-reports/assets/clinical_trial_csr_template.json +0 -51
- package/skills/clinical-reports/assets/clinical_trial_results_template.json +0 -66
- package/skills/clinical-reports/assets/clinical_trial_safety_aggregate_template.json +0 -49
- package/skills/clinical-reports/assets/consistency_manifest_template.json +0 -15
- package/skills/clinical-reports/assets/deidentification_process_checklist.json +0 -56
- package/skills/clinical-reports/assets/lab_report_template.json +0 -36
- package/skills/clinical-reports/assets/pathology_report_template.json +0 -36
- package/skills/clinical-reports/assets/provenance_manifest_template.json +0 -17
- package/skills/clinical-reports/assets/quality_review_checklist.json +0 -34
- package/skills/clinical-reports/assets/radiology_report_template.json +0 -34
- package/skills/clinical-reports/assets/research_summary_template.json +0 -36
- package/skills/clinical-reports/assets/terminology_manifest_template.json +0 -11
- package/skills/clinical-reports/assets/trial_protocol_reporting_checklist.json +0 -70
- package/skills/clinical-reports/references/case_report_guidelines.md +0 -72
- package/skills/clinical-reports/references/clinical_trial_reporting.md +0 -97
- package/skills/clinical-reports/references/data_presentation.md +0 -92
- package/skills/clinical-reports/references/diagnostic_reports_standards.md +0 -76
- package/skills/clinical-reports/references/medical_terminology.md +0 -84
- package/skills/clinical-reports/references/privacy_and_deidentification.md +0 -84
- package/skills/clinical-reports/references/professional_review.md +0 -78
- package/skills/clinical-reports/references/report_type_routing.md +0 -57
- package/skills/clinical-reports/references/safety_reporting.md +0 -109
- package/skills/clinical-reports/references/sources.md +0 -75
- package/skills/clinical-reports/scripts/_common.py +0 -263
- package/skills/clinical-reports/scripts/check_deidentification.py +0 -298
- package/skills/clinical-reports/scripts/consistency_checker.py +0 -390
- package/skills/clinical-reports/scripts/format_adverse_events.py +0 -455
- package/skills/clinical-reports/scripts/generate_report_template.py +0 -115
- package/skills/clinical-reports/scripts/provenance_validator.py +0 -263
- package/skills/clinical-reports/scripts/terminology_validator.py +0 -277
- package/skills/clinical-reports/scripts/validate_case_report.py +0 -278
- package/skills/clinical-reports/scripts/validate_trial_report.py +0 -534
- package/skills/cobrapy/references/api_quick_reference.md +0 -665
- package/skills/cobrapy/references/workflows.md +0 -600
- package/skills/consciousness-council/references/advanced-configurations.md +0 -96
- package/skills/dask/references/arrays.md +0 -495
- package/skills/dask/references/bags.md +0 -468
- package/skills/dask/references/best-practices.md +0 -277
- package/skills/dask/references/dataframes.md +0 -370
- package/skills/dask/references/futures.md +0 -541
- package/skills/dask/references/schedulers.md +0 -517
- package/skills/database-lookup/references/addgene.md +0 -38
- package/skills/database-lookup/references/alphafold.md +0 -52
- package/skills/database-lookup/references/alphavantage.md +0 -261
- package/skills/database-lookup/references/bea.md +0 -409
- package/skills/database-lookup/references/bindingdb.md +0 -85
- package/skills/database-lookup/references/biogrid.md +0 -110
- package/skills/database-lookup/references/bls.md +0 -235
- package/skills/database-lookup/references/brenda.md +0 -71
- package/skills/database-lookup/references/cbioportal.md +0 -206
- package/skills/database-lookup/references/census.md +0 -251
- package/skills/database-lookup/references/chebi.md +0 -103
- package/skills/database-lookup/references/chembl.md +0 -80
- package/skills/database-lookup/references/clinicaltrials.md +0 -86
- package/skills/database-lookup/references/clinpgx.md +0 -64
- package/skills/database-lookup/references/clinvar.md +0 -91
- package/skills/database-lookup/references/cod.md +0 -121
- package/skills/database-lookup/references/cosmic.md +0 -59
- package/skills/database-lookup/references/dailymed.md +0 -65
- package/skills/database-lookup/references/database_selection_guide.md +0 -166
- package/skills/database-lookup/references/datacommons.md +0 -237
- package/skills/database-lookup/references/dbsnp.md +0 -143
- package/skills/database-lookup/references/disgenet.md +0 -52
- package/skills/database-lookup/references/drugbank.md +0 -54
- package/skills/database-lookup/references/ecb.md +0 -191
- package/skills/database-lookup/references/emdb.md +0 -37
- package/skills/database-lookup/references/ena.md +0 -372
- package/skills/database-lookup/references/encode.md +0 -47
- package/skills/database-lookup/references/ensembl.md +0 -539
- package/skills/database-lookup/references/epa.md +0 -232
- package/skills/database-lookup/references/eurostat.md +0 -237
- package/skills/database-lookup/references/fda.md +0 -64
- package/skills/database-lookup/references/federal-reserve.md +0 -216
- package/skills/database-lookup/references/fred.md +0 -297
- package/skills/database-lookup/references/gene-ontology.md +0 -147
- package/skills/database-lookup/references/geo.md +0 -130
- package/skills/database-lookup/references/gnomad.md +0 -93
- package/skills/database-lookup/references/gtex.md +0 -136
- package/skills/database-lookup/references/gwas-catalog.md +0 -46
- package/skills/database-lookup/references/hca.md +0 -35
- package/skills/database-lookup/references/hpo.md +0 -48
- package/skills/database-lookup/references/human-protein-atlas.md +0 -57
- package/skills/database-lookup/references/interpro.md +0 -120
- package/skills/database-lookup/references/jaspar.md +0 -50
- package/skills/database-lookup/references/kegg.md +0 -78
- package/skills/database-lookup/references/lincs-l1000.md +0 -68
- package/skills/database-lookup/references/materials-project.md +0 -123
- package/skills/database-lookup/references/metabolomics-workbench.md +0 -98
- package/skills/database-lookup/references/monarch.md +0 -46
- package/skills/database-lookup/references/mousemine.md +0 -40
- package/skills/database-lookup/references/nasa-exoplanet-archive.md +0 -112
- package/skills/database-lookup/references/nasa.md +0 -121
- package/skills/database-lookup/references/ncbi-gene.md +0 -64
- package/skills/database-lookup/references/ncbi-protein.md +0 -104
- package/skills/database-lookup/references/ncbi-taxonomy.md +0 -121
- package/skills/database-lookup/references/nist.md +0 -105
- package/skills/database-lookup/references/noaa.md +0 -199
- package/skills/database-lookup/references/omim.md +0 -114
- package/skills/database-lookup/references/opentargets.md +0 -459
- package/skills/database-lookup/references/openweathermap.md +0 -255
- package/skills/database-lookup/references/pdb.md +0 -121
- package/skills/database-lookup/references/pride.md +0 -74
- package/skills/database-lookup/references/pubchem.md +0 -145
- package/skills/database-lookup/references/quickgo.md +0 -45
- package/skills/database-lookup/references/reactome.md +0 -140
- package/skills/database-lookup/references/retrieval-contract.md +0 -123
- package/skills/database-lookup/references/rummageo.md +0 -32
- package/skills/database-lookup/references/sdss.md +0 -130
- package/skills/database-lookup/references/sec-edgar.md +0 -315
- package/skills/database-lookup/references/simbad.md +0 -405
- package/skills/database-lookup/references/sra.md +0 -149
- package/skills/database-lookup/references/string.md +0 -283
- package/skills/database-lookup/references/tcga-gdc.md +0 -58
- package/skills/database-lookup/references/treasury.md +0 -215
- package/skills/database-lookup/references/ucsc-genome.md +0 -135
- package/skills/database-lookup/references/uniprot.md +0 -283
- package/skills/database-lookup/references/usgs.md +0 -260
- package/skills/database-lookup/references/uspto.md +0 -130
- package/skills/database-lookup/references/who.md +0 -283
- package/skills/database-lookup/references/worldbank.md +0 -239
- package/skills/database-lookup/references/zinc.md +0 -202
- package/skills/datamol/references/conformers_module.md +0 -131
- package/skills/datamol/references/core_api.md +0 -136
- package/skills/datamol/references/core_workflows.md +0 -451
- package/skills/datamol/references/descriptors_viz.md +0 -195
- package/skills/datamol/references/fragments_scaffolds.md +0 -174
- package/skills/datamol/references/io_module.md +0 -112
- package/skills/datamol/references/reactions_data.md +0 -218
- package/skills/datamol/references/workflow_patterns.md +0 -104
- package/skills/deepchem/references/api_reference.md +0 -305
- package/skills/deepchem/references/core_capabilities.md +0 -276
- package/skills/deepchem/references/typical_workflows.md +0 -109
- package/skills/deepchem/references/workflows.md +0 -491
- package/skills/deepchem/scripts/graph_neural_network.py +0 -350
- package/skills/deepchem/scripts/predict_solubility.py +0 -223
- package/skills/deepchem/scripts/transfer_learning.py +0 -443
- package/skills/deepspot-m/references/api.md +0 -186
- package/skills/deepspot-m/references/whole_slide.md +0 -174
- package/skills/deeptools/assets/quick_reference.md +0 -65
- package/skills/deeptools/references/core_workflows.md +0 -134
- package/skills/deeptools/references/effective_genome_sizes.md +0 -118
- package/skills/deeptools/references/normalization_methods.md +0 -424
- package/skills/deeptools/references/tools_reference.md +0 -569
- package/skills/deeptools/references/workflows.md +0 -476
- package/skills/deeptools/scripts/validate_files.py +0 -195
- package/skills/deeptools/scripts/workflow_generator.py +0 -520
- package/skills/depmap/references/dependency_analysis.md +0 -178
- package/skills/dhdna-profiler/references/advanced-profiling.md +0 -72
- package/skills/diffdock/assets/batch_template.csv +0 -4
- package/skills/diffdock/assets/custom_inference_config.yaml +0 -94
- package/skills/diffdock/references/confidence_and_limitations.md +0 -182
- package/skills/diffdock/references/parameters_reference.md +0 -173
- package/skills/diffdock/references/workflows_examples.md +0 -401
- package/skills/diffdock/scripts/analyze_results.py +0 -346
- package/skills/diffdock/scripts/prepare_batch_csv.py +0 -257
- package/skills/diffdock/scripts/setup_check.py +0 -283
- package/skills/dnanexus-integration/references/app-development.md +0 -371
- package/skills/dnanexus-integration/references/authentication.md +0 -226
- package/skills/dnanexus-integration/references/configuration.md +0 -444
- package/skills/dnanexus-integration/references/data-operations.md +0 -474
- package/skills/dnanexus-integration/references/job-execution.md +0 -482
- package/skills/dnanexus-integration/references/operations-and-troubleshooting.md +0 -506
- package/skills/dnanexus-integration/references/python-sdk.md +0 -548
- package/skills/dnanexus-integration/references/sources.md +0 -168
- package/skills/dnanexus-integration/references/workflow-languages.md +0 -292
- package/skills/dnanexus-integration/scripts/inspect_dxpy.py +0 -366
- package/skills/dnanexus-integration/scripts/validate_dxapp.py +0 -796
- package/skills/docx/LICENSE.txt +0 -30
- package/skills/docx/scripts/__init__.py +0 -1
- package/skills/docx/scripts/accept_changes.py +0 -135
- package/skills/docx/scripts/comment.py +0 -368
- package/skills/docx/scripts/merge_runs.py +0 -310
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- package/skills/xlsx/scripts/office/schemas/microsoft/wml-cid-2016.xsd +0 -13
- package/skills/xlsx/scripts/office/schemas/microsoft/wml-sdtdatahash-2020.xsd +0 -4
- package/skills/xlsx/scripts/office/schemas/microsoft/wml-symex-2015.xsd +0 -8
- package/skills/xlsx/scripts/office/soffice.py +0 -232
- package/skills/xlsx/scripts/office/validate.py +0 -173
- package/skills/xlsx/scripts/office/validators/__init__.py +0 -15
- package/skills/xlsx/scripts/office/validators/base.py +0 -875
- package/skills/xlsx/scripts/office/validators/docx.py +0 -466
- package/skills/xlsx/scripts/office/validators/pptx.py +0 -441
- package/skills/xlsx/scripts/office/validators/redlining.py +0 -299
- package/skills/xlsx/scripts/recalc.py +0 -308
- package/skills/zarr-python/references/api_reference.md +0 -162
- package/skills/zarr-python/references/chunking_and_compression.md +0 -138
- package/skills/zarr-python/references/integration.md +0 -147
- package/skills/zarr-python/references/performance_and_patterns.md +0 -198
- package/skills/zarr-python/references/storage_backends.md +0 -91
- package/skills/zarr-python/references/v3_migration.md +0 -127
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# Labware adapters, holders, and racks
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Parts that receive standard consumables: microplates, cuvettes, tubes, slides, dishes.
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The governing principle: **where a published standard exists, design to the standard; where it
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does not, require a measurement.** Microplate footprints are standardised. Well geometry, skirt
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profiles, tube dimensions, and lid fits are not.
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Verified dimensions live in `assets/standards.json`. Query them rather than copying numbers:
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```bash
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python scripts/check.py standards --show slas-microplate-footprint
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```
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## Microplates (ANSI/SLAS 1-4)
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Four documents split the plate geometry. All are ANSI-approved and were reaffirmed in 2012.
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| Document | Governs | Key numbers |
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| --- | --- | --- |
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| ANSI/SLAS 1-2004 | Footprint | 127.76 x 85.48 mm ±0.25; corner radius 3.18 ±1.6 mm |
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| ANSI/SLAS 2-2004 | Height | 14.35 ±0.25 mm, resting plane to top of perimeter wells |
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| ANSI/SLAS 3-2004 | Bottom outside flange | Short 2.41, medium 6.10, tall 7.62 mm, each ±0.38 |
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| ANSI/SLAS 4-2004 | Well positions | 96-well: 9.0 mm pitch, A1 at 14.38 mm from left, 11.24 mm from top |
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### Designing a plate pocket
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Three traps, in the order people fall into them.
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**1. Design to maximum material, not to nominal.** A plate at the top of tolerance is
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127.76 + 0.25 = 128.01 mm. A pocket cut at 127.76 + clearance will jam on roughly half the plates
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you try. Compute:
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```python
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plate_l_mm = 127.76 # ANSI/SLAS 1-2004 nominal
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plate_tol_mm = 0.25 # ANSI/SLAS 1-2004
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fit_clearance_mm = 0.40 # per side; FDM, see fabrication-limits.md
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pocket_l_mm = plate_l_mm + plate_tol_mm + 2 * fit_clearance_mm # 128.81
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```
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**2. The corner radius tolerance is enormous — and it bounds the pocket radius from above,
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not below.** 3.18 ±1.6 mm means a real plate corner is anywhere from 1.58 to 4.78 mm. Get the
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direction right: a plate corner is **convex**, a pocket fillet is **concave material bulging
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inward**, so a *sharp* internal pocket corner always clears a rounded plate — the unused corner is
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empty space. It is a pocket fillet *larger* than the plate's corner radius that binds: the bulge
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occupies space the plate needs. Sizing the fillet to the plate's maximum corner radius is
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therefore exactly backwards — it binds every plate except those at the top of the corner
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tolerance.
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The safe options, best first:
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- **Corner relief** (a small slot or bore cut past each corner) — always clears, prints and mills
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cleanly, and is the standard fix.
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- **Fillet no larger than the plate's minimum corner radius** (1.58 mm for SLAS plates) — clears
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every conforming plate in every position.
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- A larger fillet only if `R ≤ r_min + ~3.4 × per-side clearance` — the geometry only recovers the
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intrusion when the plate stays roughly centred, so treat this as a last resort and say so.
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```python
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with BuildPart() as pocket:
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# ... pocket geometry ...
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# relief bores just outside each pocket corner: clears any conforming corner radius
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with Locations(*corner_relief_centres()):
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Hole(radius=2.0)
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```
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**3. Height depends on the flange, not just the plate.** ANSI/SLAS 3 standardises three flange
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heights. A carrier that grips the flange must be told which one. Ask; do not assume medium.
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### Well grid
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For a part that must reach individual wells — a magnet block, a lid with access holes, a light
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guide — lay out from the plate's outline corner, not from the plate centre:
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```python
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a1_x_mm, a1_y_mm, pitch_mm = 14.38, 11.24, 9.0 # ANSI/SLAS 4-2004, 96-well
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locations = [
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(a1_x_mm + pitch_mm * col, a1_y_mm + pitch_mm * row)
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for row in range(8) for col in range(12)
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]
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```
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The standard's positional tolerance is a **0.70 mm diameter zone** around each nominal centre, not
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a ±0.70 mm band. A feature that must clear every well needs at least 0.35 mm of radial margin on
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top of your own process tolerance.
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384-well pitch is 4.5 mm and 1536-well pitch is 2.25 mm. **The A1 offsets for those formats in
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`standards.json` are marked unverified** — they were derived, not read from the document. Read
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ANSI/SLAS 4-2004 before relying on them.
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### What the standards do not fix
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Well diameter, well depth, well bottom shape (flat, round, conical), skirt height, lid geometry,
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optical bottom thickness, and deep-well plate height. All vary by manufacturer and product line.
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If the part touches any of these, get the vendor drawing or measure it.
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## Cuvettes
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The standard macro cuvette is a convention rather than a published standard, but it is close to
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universal: **12.5 x 12.5 mm external, 45 mm tall, 1.25 mm wall, 10 mm optical path**.
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Design notes:
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- Holders should be generous or compliant. Because no document fixes the tolerance, a 0.1 mm
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interference fit designed against nominal will fail on some suppliers' cuvettes.
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- Semi-micro and micro cuvettes keep the 12.5 mm external footprint but change internal geometry
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and often height. A holder designed for the external footprint accommodates all of them; one
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designed around the sample volume does not.
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- Cuvettes are usually held with a spring or leaf on one face so the two optical faces register
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against fixed datums. Copy that: locate on two adjacent faces, preload from the opposite corner.
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A four-sided pocket with clearance lets the cuvette rotate and shifts the path length.
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- **Never print the optical path.** Printed surfaces scatter. The cuvette provides the optical
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faces; the holder provides position only, and must not obstruct the beam window.
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## Tubes
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Tube dimensions are **not standardised** and differ measurably between suppliers, and often
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between product lines from the same supplier. Approximate outside diameters near the tube rim:
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| Tube | Approximate OD | Note |
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| --- | --- | --- |
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| 0.2 mL PCR | 6 mm | Often supplied in strips or as a 96-format plate |
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| 1.5 mL microcentrifuge | 11 mm | Rim is wider than the body; the body tapers |
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| 2.0 mL microcentrifuge | 11 mm | Same rim as 1.5 mL, taller body |
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| 15 mL conical | 17 mm | Cap is wider than the tube |
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| 50 mL conical | 30 mm | Cap is wider than the tube |
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**Treat every number in this table as a starting point for a first article, not a design input.**
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Ask the user for the supplier and catalogue number, or ask them to measure with calipers. Then
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design a rack that holds the tube by the **rim or the cap**, which is dimensionally stable, rather
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than by the tapered body, which is not.
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For a rack, the useful pattern is a through-hole sized to the body plus clearance and a counterbore
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that catches the rim, so the tube hangs rather than bottoms out.
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## Microscope slides and coverslips
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Standard slide: **75 x 25 mm, 1.0 mm thick** (ISO 8037-1 covers slide dimensions; thickness classes
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vary, and 1.0-1.2 mm is typical). Coverslips are specified by thickness number, not dimension:
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#1 is roughly 0.13-0.17 mm and #1.5 roughly 0.16-0.19 mm.
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Objective working distance is unforgiving. A holder that adds even 0.2 mm under the slide can put
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the sample outside a high-NA objective's working distance. Design slide holders so the slide
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registers directly against the stage datum, with the holder clamping from above.
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## Petri dishes and stage inserts
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Standard dish outside diameters are approximately 35, 60, 90, and 100 mm, but the flange profile
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and lid fit vary. Dishes are also slightly out of round. Locate on three points rather than a
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continuous circular pocket: a three-point nest is insensitive to ovality, a close-fitting bore is
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not.
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For stage inserts, the interface that matters is the **microscope stage opening**, which is
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instrument-specific and must be measured. Many stages accept a standard SLAS-footprint insert;
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confirm before assuming it.
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## Checks to run
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Declare the pocket in the model's `interfaces()` and let the check read it:
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```bash
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python scripts/gen.py carrier_model.py --outdir out/
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python scripts/check.py interfaces out/carrier.manifest.json
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```
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**Do not point `check.py fit` at the carrier's STEP.** `fit` measures the outer bounding box, which
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for a carrier is the outside of its walls — 6 mm larger than the pocket here — so it fails against
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the plate footprint no matter how correct the pocket is. The dimension that matters is internal, so
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it has to be declared, not measured from the envelope.
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To check the number by hand instead:
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```bash
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python scripts/check.py fit --standard slas-microplate-footprint \
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--intent envelope --clearance 0.8 --value footprint_length=128.81
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```
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`--intent envelope` checks one-sided against maximum material condition, and `--clearance` is the
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total intended clearance: 0.40 mm per side is 0.80 mm. Passing means the pocket is the size you
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intended, not that the plate fits — only a test print shows that.
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Then always run `snapshot.py` and confirm the pocket is on the face you meant.
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## Sources
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- ANSI/SLAS 1-2004 (R2012) Footprint Dimensions — <https://www.slas.org/SLAS/assets/File/public/standards/ANSI_SLAS_1-2004_FootprintDimensions.pdf>
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- ANSI/SLAS 2-2004 (R2012) Height Dimensions — <https://www.slas.org/SLAS/assets/File/public/standards/ANSI_SLAS_2-2004_HeightDimensions.pdf>
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- ANSI/SLAS 3-2004 (R2012) Bottom Outside Flange Dimensions — <https://www.slas.org/SLAS/assets/File/public/standards/ANSI_SLAS_3-2004_BottomOutsideFlangeDimensions.pdf>
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- ANSI/SLAS 4-2004 (R2012) Well Positions — <https://www.slas.org/SLAS/assets/File/public/standards/ANSI_SLAS_4-2004_WellPositions.pdf>
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- SLAS microplate standards overview — <https://www.slas.org/education/ansi-slas-microplate-standards/>
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# Microfluidic chips, molds, and flow cells
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Channel networks, soft-lithography molds, printed chips, gaskets, and manifolds.
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## First: decide what you are actually modelling
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This is the error that wastes the most time in microfluidic CAD. Three different objects get
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called "the chip":
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| Object | Channels are | Made by |
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| --- | --- | --- |
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| **Mold / master** | **Raised ridges** (positive relief) | Photolithography on a wafer, SLA print, or micromilling |
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| **Cast chip** | **Recessed grooves** (negative of the mold) | PDMS cast against the mold, then bonded to a substrate |
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| **Directly-fabricated chip** | **Recessed grooves or enclosed lumens** | Printed, milled, or laser-cut directly |
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A model that is correct as a chip is exactly wrong as a mold. Put the polarity in the module
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docstring and in a named parameter, and **verify it numerically, not by eye**: inverted polarity
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is invisible in the bounding box, the volume, and the validity check — and at typical channel
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scale (a 0.3 mm ridge on a 40+ mm part) it is invisible in an outline render too, because raised
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and recessed features draw the same edges. Declare it as geometry checks instead
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(`references/build123d-patterns.md`): a `material` region where the ridge must stand above the
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casting surface, and a `clear` region over the rest of that layer — a groove fails the first,
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an inverted full-area layer fails the second. For a one-off question,
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`check.py probe <step> --box ... --expect material` answers it without editing the model. State
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the measured relief height in the report. Use the snapshot for layout and connectivity, which
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it does show well.
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```python
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polarity = "mold" # "mold" = raised ridges; "chip" = recessed grooves
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```
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If casting PDMS, the mold also needs a **surrounding wall or a casting frame** to contain the
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uncured polymer, and enough flat land around the features for the cast part to release.
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## Channel cross-section and aspect ratio
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Channels are usually rectangular because that is what planar fabrication produces. Two failure
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modes bound the aspect ratio, and both are geometric:
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- **Roof sag / collapse** — a channel much wider than it is tall has an unsupported ceiling. In
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PDMS the roof bows down and can stick to the floor. Commonly cited guidance keeps
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**width : height below roughly 10 : 1**; wide channels need support pillars.
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- **Sidewall collapse** — a mold ridge much taller than it is wide falls over or fails to release.
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Keep **height : width below roughly 10 : 1** on the mold.
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Treat both as rules of thumb, not guarantees: the real limits depend on PDMS mixing ratio, cure
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schedule, and applied pressure. For anything load-bearing or high-pressure, prototype.
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Also keep **channel-to-channel spacing at least the channel height**, so the wall between two
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channels does not deflect or leak, and leave a flat **bonding land** — typically 1 mm or more of
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uninterrupted flat surface around the network perimeter — for plasma or adhesive bonding.
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## Minimum features by process
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55
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Achievable feature size drives the entire design, and the range across processes is three orders
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of magnitude. Confirm against your specific tool before committing.
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|
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| Process | Practical minimum channel | Notes |
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| --- | --- | --- |
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| SU-8 photolithography | ~1-10 µm wide, 1-200+ µm tall | The reference process for soft lithography. Feature height is set by spin speed and resist grade. |
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| Two-photon / µSLA | ~10-50 µm | Small build volume, slow, expensive. |
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| Desktop SLA / DLP | ~200-500 µm | Uncured resin is very hard to clear from smaller lumens. Enclosed channels below ~0.5 mm frequently print blocked. |
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| Micromilling | ~100 µm | Set by end-mill diameter; depth limited by tool aspect ratio. Leaves tool marks that scatter light. |
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| FDM | Not suitable for sealed channels | Layer porosity leaks. Use only for holders and manifolds. |
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| Laser-cut film / gasket | ~200 µm | Excellent for stacked-layer devices and gaskets. |
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|
67
|
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**Design enclosed printed channels for drainage.** Every lumen needs a path for uncured resin to
|
|
68
|
-
escape, and orientation on the build plate determines whether it drains. If the user is printing,
|
|
69
|
-
say which way up.
|
|
70
|
-
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|
71
|
-
## Ports and tubing
|
|
72
|
-
|
|
73
|
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The port is where most chips leak. Options, roughly in order of how common they are in a research
|
|
74
|
-
lab:
|
|
75
|
-
|
|
76
|
-
- **Direct tubing insertion** — a bore slightly *under* the tubing OD so the tubing seals by
|
|
77
|
-
interference. For 1/16 inch OD tubing (1.5875 mm), a bore around 1.5 mm in PDMS is typical. This
|
|
78
|
-
works in elastomer and fails in rigid printed parts, which crack instead of gripping.
|
|
79
|
-
- **Luer taper** — the standard syringe interface, a **6% taper** (ISO 80369-7 supersedes the
|
|
80
|
-
legacy ISO 594 series for medical use). Convenient, low pressure only. If you model a Luer taper,
|
|
81
|
-
get the profile from the standard, not from memory.
|
|
82
|
-
- **Threaded fittings** — flat-bottom **1/4-28 UNF** is the common lab standard for low-pressure
|
|
83
|
-
fluidics; **10-32 coned** is used at higher pressures. These need a tapped or heat-set-insert
|
|
84
|
-
port and a matching flat sealing face.
|
|
85
|
-
- **Barbs** — reliable with soft tubing and a clamp, bulky.
|
|
86
|
-
|
|
87
|
-
Whichever you choose, the sealing surface must be **flat and normal to the port axis**. A port
|
|
88
|
-
face left at a printed layer angle will not seal.
|
|
89
|
-
|
|
90
|
-
## Dead volume
|
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|
-
|
|
92
|
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Dead volume dominates the response time of any perfusion or gradient device, and it is trivially
|
|
93
|
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computable, so compute it rather than estimating:
|
|
94
|
-
|
|
95
|
-
```
|
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96
|
-
V = pi * r^2 * L # round tubing / bore
|
|
97
|
-
V = w * h * L # rectangular channel
|
|
98
|
-
```
|
|
99
|
-
|
|
100
|
-
Report the volume of every connecting bore alongside the channel network volume. A 20 mm long
|
|
101
|
-
1 mm bore holds ~15.7 µL, which is often larger than the entire channel network it feeds.
|
|
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-
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|
103
|
-
## Flow regime sanity check
|
|
104
|
-
|
|
105
|
-
Microfluidic flow is almost always laminar, but state it rather than assuming:
|
|
106
|
-
|
|
107
|
-
```
|
|
108
|
-
Re = rho * v * D_h / mu
|
|
109
|
-
D_h = 2 * w * h / (w + h) # hydraulic diameter, rectangular channel
|
|
110
|
-
```
|
|
111
|
-
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|
112
|
-
For water in a 100 µm channel at 1 mm/s, Re is of order 0.1 — deeply laminar, so mixing is
|
|
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|
-
diffusive only. If the design depends on mixing, it needs a mixer geometry (serpentine,
|
|
114
|
-
herringbone, or split-and-recombine); relying on turbulence will not work at these scales.
|
|
115
|
-
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116
|
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Pressure drop for a rectangular channel scales steeply with the smaller dimension. **Halving
|
|
117
|
-
channel height raises pressure drop by roughly an order of magnitude.** Check that the intended
|
|
118
|
-
pump or syringe can actually deliver it before finalising the cross-section.
|
|
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|
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|
|
120
|
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## Material and optical constraints
|
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|
-
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122
|
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- **PDMS** absorbs small hydrophobic molecules and is gas-permeable. Both are sometimes features
|
|
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|
-
(oxygenation in organ-on-chip) and sometimes fatal to an assay (drug studies).
|
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|
-
- **SLA resins** are frequently cytotoxic uncured and often still after a nominal cure. For cell
|
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|
-
work, require post-cure plus a documented biocompatibility check, or use a different process.
|
|
126
|
-
See `references/fabrication-limits.md`.
|
|
127
|
-
- **Autofluorescence** matters for any fluorescence readout. Most printed resins autofluoresce
|
|
128
|
-
strongly. Image through glass or a thin COC/COP film, not through printed material.
|
|
129
|
-
- **Optical path**: printed and milled surfaces scatter. Any imaging window should be a bonded
|
|
130
|
-
coverslip or film, and the model must specify its thickness so the objective working distance
|
|
131
|
-
works out.
|
|
132
|
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|
|
133
|
-
## Checks to run
|
|
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|
-
|
|
135
|
-
```bash
|
|
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|
-
python scripts/gen.py chip_model.py --outdir out/
|
|
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|
-
python scripts/check.py facts out/chip.step
|
|
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|
-
python scripts/snapshot.py out/chip.step --out out/chip.png
|
|
139
|
-
```
|
|
140
|
-
|
|
141
|
-
`facts` gives the volume; compare it against your hand-computed channel volume as an independent
|
|
142
|
-
check that the network is actually open and connected. A network modelled as a solid rather than a
|
|
143
|
-
cavity shows up immediately as a volume far larger than expected.
|
|
144
|
-
|
|
145
|
-
Then read the snapshot and confirm, explicitly:
|
|
146
|
-
|
|
147
|
-
1. **Polarity** — ridges for a mold, grooves for a chip.
|
|
148
|
-
2. Every port lands on the channel it should, and passes fully through to the surface.
|
|
149
|
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3. The bonding land is continuous around the network.
|
|
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|
-
4. No channel has been closed off or consumed by a fillet.
|
|
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|
-
|
|
152
|
-
## Sources
|
|
153
|
-
|
|
154
|
-
- ISO 80369-7 (Luer connectors for intravascular applications) supersedes the ISO 594 series.
|
|
155
|
-
Obtain the taper profile from the standard itself.
|
|
156
|
-
- Aspect-ratio and spacing guidance here is standard soft-lithography practice; the numerical
|
|
157
|
-
limits are rules of thumb and depend on material and process. Prototype before committing.
|
|
@@ -1,148 +0,0 @@
|
|
|
1
|
-
# Optomechanical mounts and breadboard hardware
|
|
2
|
-
|
|
3
|
-
Parts that bolt to an optical table, join a cage system, hold an optic or a sample in a beam path,
|
|
4
|
-
or carry a camera or objective.
|
|
5
|
-
|
|
6
|
-
Verified dimensions are in `assets/standards.json`:
|
|
7
|
-
|
|
8
|
-
```bash
|
|
9
|
-
python scripts/check.py standards --show optical-breadboard-metric
|
|
10
|
-
python scripts/check.py standards --show cage-system-30mm
|
|
11
|
-
python scripts/check.py standards --show sm1-lens-tube-thread
|
|
12
|
-
```
|
|
13
|
-
|
|
14
|
-
## Ask which system before you model anything
|
|
15
|
-
|
|
16
|
-
**Metric and imperial optical hardware are not interchangeable, and the difference is small enough
|
|
17
|
-
to look like a rounding error and large enough to prevent assembly.**
|
|
18
|
-
|
|
19
|
-
| | Metric | Imperial |
|
|
20
|
-
| --- | --- | --- |
|
|
21
|
-
| Grid pitch | 25.0 mm | 25.4 mm (1 inch) |
|
|
22
|
-
| Tapped hole | M6 x 1.0 | 1/4-20 UNC |
|
|
23
|
-
| Typical border | 12.5 mm | 12.7 mm |
|
|
24
|
-
|
|
25
|
-
Over a four-hole span the grids differ by **1.6 mm** — far more than any clearance hole absorbs.
|
|
26
|
-
There is no way to infer which the user has from the request. Ask. If the answer is unavailable,
|
|
27
|
-
model the mounting features as **slots along the bolt line** rather than round holes, which
|
|
28
|
-
tolerates both, and say that is what you did and why.
|
|
29
|
-
|
|
30
|
-
## Mounting to the table
|
|
31
|
-
|
|
32
|
-
- Use **clearance holes, not tapped holes**, in the part. The table is tapped; the part is
|
|
33
|
-
clearanced. For M6 use 6.6 mm (normal fit) in a printed part rather than 6.4 mm — printed holes
|
|
34
|
-
come out undersize.
|
|
35
|
-
- **Counterbore for the screw head** if the part surface must stay clear: roughly 11 mm diameter
|
|
36
|
-
for an M6 socket head cap screw, 11.2 mm for 1/4-20.
|
|
37
|
-
- **Never rely on more than two holes to locate a part.** Grid tolerance plus print tolerance means
|
|
38
|
-
a rigid four-hole pattern will bind. Round hole + slot is the standard fix: one hole locates, the
|
|
39
|
-
slot takes up the error.
|
|
40
|
-
- Printed parts are compliant. For anything where pointing stability matters, a printed mount is a
|
|
41
|
-
prototyping aid, not a final part — thermal drift and creep in polymer are large compared with
|
|
42
|
-
optical alignment tolerances. Say so when recommending one.
|
|
43
|
-
|
|
44
|
-
## Posts and pedestals
|
|
45
|
-
|
|
46
|
-
Common conventions, which vary by vendor — **confirm against the catalogue before use**:
|
|
47
|
-
|
|
48
|
-
- Imperial posts are Ø1/2 inch (12.7 mm), typically tapped 8-32 at one end with a 1/4-20 stud or
|
|
49
|
-
clearance at the other.
|
|
50
|
-
- Metric posts are Ø12 mm, typically tapped M4 with an M6 interface to the table.
|
|
51
|
-
- A post-holder plus post is height-adjustable but adds a compliant joint; a pedestal or a
|
|
52
|
-
solid machined riser is stiffer.
|
|
53
|
-
|
|
54
|
-
**Beam height** is a project-wide constant, not a per-part choice. Every mount on the table must
|
|
55
|
-
put its optic at the same height. Common conventions are 3 inches (76.2 mm) or 100 mm, but this is
|
|
56
|
-
a lab-by-lab choice. Ask for the number, define it once as `beam_height_mm`, and derive every
|
|
57
|
-
mount's optic centre from it.
|
|
58
|
-
|
|
59
|
-
## 30 mm cage system
|
|
60
|
-
|
|
61
|
-
The dominant convention for small free-space assemblies:
|
|
62
|
-
|
|
63
|
-
- **Rod spacing 30.0 mm** on a square, centred on the optical axis.
|
|
64
|
-
- **Rods Ø6 mm** (ER series).
|
|
65
|
-
- Standard cage plates are 0.35 inch (8.9 mm) thick.
|
|
66
|
-
|
|
67
|
-
For a custom cage plate: place four bores on a 30 mm square, put the aperture at the **centroid**
|
|
68
|
-
of those four bores, and bore them for a free-sliding fit **at your process's clearance**
|
|
69
|
-
(fabrication-limits.md): about 6.2 mm CNC, 6.4 mm SLA, 6.8 mm FDM. 6.1 mm is a reamed-metal
|
|
70
|
-
number — printed bores come out undersize, and four bores on a common square over-constrain each
|
|
71
|
-
other, so tighter is not better here. A cage plate that binds on the rods is worse than useless
|
|
72
|
-
because it transmits stress into the whole assembly.
|
|
73
|
-
|
|
74
|
-
Cage plates stack along the rods, so a custom plate's thickness directly consumes optical path
|
|
75
|
-
length. Budget it.
|
|
76
|
-
|
|
77
|
-
## Lens tube threads (SM series)
|
|
78
|
-
|
|
79
|
-
**SM1 is a 1.035 inch-40 thread**, which holds Ø1 inch (25.4 mm) optics. That is a **0.635 mm
|
|
80
|
-
pitch**.
|
|
81
|
-
|
|
82
|
-
**Do not print SM threads.** A 0.635 mm pitch is at or below the practical resolution of FDM and
|
|
83
|
-
marginal on desktop SLA; a printed SM1 thread will either not engage or will gall and shed
|
|
84
|
-
particles into the beam path. Instead:
|
|
85
|
-
|
|
86
|
-
- bore a clearance hole and use a purchased SM1 adapter or retaining ring, or
|
|
87
|
-
- design for a threaded metal insert, or
|
|
88
|
-
- clamp the optic directly with a retaining flange and screws.
|
|
89
|
-
|
|
90
|
-
If the design truly requires a printed thread, say explicitly that it needs test printing and is
|
|
91
|
-
likely to fail.
|
|
92
|
-
|
|
93
|
-
## Holding an optic
|
|
94
|
-
|
|
95
|
-
- **Never clamp an optic on its clear aperture.** Contact only the outer annulus of the face or the
|
|
96
|
-
edge. Define `clear_aperture_mm` as a named parameter and confirm in the snapshot that nothing
|
|
97
|
-
intrudes on it.
|
|
98
|
-
- Three-point contact is kinematically correct and does not deform the optic. A continuous
|
|
99
|
-
circular seat over-constrains it and induces stress birefringence, which matters for
|
|
100
|
-
polarisation work.
|
|
101
|
-
- Leave clearance for thermal expansion. A metal-in-polymer mount that is a press fit at 20 °C can
|
|
102
|
-
crack or bind across a temperature swing.
|
|
103
|
-
- Retaining forces should be light and distributed. A single set screw pressing on glass is a way
|
|
104
|
-
to chip glass.
|
|
105
|
-
|
|
106
|
-
## Stray light and scatter
|
|
107
|
-
|
|
108
|
-
Geometry is not the whole design here, and a STEP file cannot show any of this:
|
|
109
|
-
|
|
110
|
-
- Printed surfaces scatter strongly. Any surface that sees the beam should be baffled, angled away
|
|
111
|
-
from the optical axis, or treated.
|
|
112
|
-
- **Black does not mean non-reflective.** Black resin and black filament are often quite specular.
|
|
113
|
-
Specify a genuinely absorbing surface treatment where it matters.
|
|
114
|
-
- Thread and layer lines act as diffraction structures near a focus.
|
|
115
|
-
- For fluorescence work, printed material near the sample can autofluoresce into the detection
|
|
116
|
-
path.
|
|
117
|
-
|
|
118
|
-
Flag these to the user; do not silently assume a printed enclosure is light-tight.
|
|
119
|
-
|
|
120
|
-
## Checks to run
|
|
121
|
-
|
|
122
|
-
```bash
|
|
123
|
-
python scripts/gen.py mount_model.py --outdir out/
|
|
124
|
-
python scripts/check.py facts out/mount.step
|
|
125
|
-
python scripts/check.py interfaces out/mount.manifest.json
|
|
126
|
-
python scripts/snapshot.py out/mount.step --out out/mount.png
|
|
127
|
-
```
|
|
128
|
-
|
|
129
|
-
Declare the grid pitch, rod spacing, and bore diameters in the model's `interfaces()` against
|
|
130
|
-
`optical-breadboard-metric`, `optical-breadboard-imperial`, or `cage-system-30mm`, so the check
|
|
131
|
-
catches a 25.0-for-25.4 substitution rather than leaving it to a reader.
|
|
132
|
-
|
|
133
|
-
There is still **no automatic bolt-pattern check** — the interface check compares dimensions, not
|
|
134
|
-
hole positions. Compute the pattern in the model from a named `grid_pitch_mm` constant, and confirm
|
|
135
|
-
in the snapshot that:
|
|
136
|
-
|
|
137
|
-
1. All mounting holes are present and pass fully through.
|
|
138
|
-
2. The optic aperture is centred where you intended, and unobstructed.
|
|
139
|
-
3. Counterbores are on the accessible face.
|
|
140
|
-
4. Nothing intrudes into the clear aperture or the beam path.
|
|
141
|
-
|
|
142
|
-
## Sources
|
|
143
|
-
|
|
144
|
-
- Thorlabs imperial and metric threading — <https://www.thorlabs.com/imperial-and-metric-threading>
|
|
145
|
-
- Thorlabs standard 30 mm cage plates — <https://www.thorlabs.com/newgrouppage9.cfm?objectgroup_ID=2273>
|
|
146
|
-
- Thorlabs SM1 lens tube compatible cage plates — <https://www.thorlabs.com/newgrouppage9.cfm?objectgroup_id=4114>
|
|
147
|
-
- Post dimensions, beam heights, and vendor-specific thread conventions in this file are common
|
|
148
|
-
conventions rather than published standards. Confirm against the catalogue.
|
|
@@ -1,132 +0,0 @@
|
|
|
1
|
-
# Pre-fabrication validation checklist
|
|
2
|
-
|
|
3
|
-
Work through this before telling a user a part is ready to fabricate. Each item names the failure
|
|
4
|
-
it catches, because a checklist without consequences gets skipped.
|
|
5
|
-
|
|
6
|
-
## 1. Provenance
|
|
7
|
-
|
|
8
|
-
- [ ] The STEP was produced by `gen.py` from the current model source.
|
|
9
|
-
*Catches: a stale artifact that no longer matches the code you just edited.*
|
|
10
|
-
- [ ] A `*.manifest.json` exists alongside it, and its `source.sha256` matches the model file.
|
|
11
|
-
*Catches: silently editing an exported STEP, which makes the design unreproducible.*
|
|
12
|
-
- [ ] The manifest's `interfaces` block lists every dimension a bundled standard covers, and its
|
|
13
|
-
values are the ones the model computed after any `--param` override. Empty is correct only
|
|
14
|
-
when nothing on the part mates with a bundled standard — and then every interface dimension
|
|
15
|
-
is named as unchecked in the report instead.
|
|
16
|
-
*Catches: a static `INTERFACES` list frozen at import, recording pre-override numbers; and
|
|
17
|
-
an interface that silently escaped checking.*
|
|
18
|
-
- [ ] Every parameter in the model is named with units.
|
|
19
|
-
*Catches: the bare `12.7` nobody can later identify as half an inch.*
|
|
20
|
-
|
|
21
|
-
```bash
|
|
22
|
-
python scripts/gen.py part_model.py --outdir out/
|
|
23
|
-
```
|
|
24
|
-
|
|
25
|
-
## 2. Geometry is sound
|
|
26
|
-
|
|
27
|
-
- [ ] `is_valid` is true.
|
|
28
|
-
*Catches: self-intersecting or non-manifold solids that slicers and CAM silently mangle.*
|
|
29
|
-
- [ ] `solid_count` is what you expect — usually 1.
|
|
30
|
-
*Catches: a boolean that failed and left two disjoint lumps, or a feature floating free of
|
|
31
|
-
the body.*
|
|
32
|
-
- [ ] Volume is plausible for the part's size and wall thickness.
|
|
33
|
-
*Catches: a cavity modelled solid, or a subtract that did nothing.*
|
|
34
|
-
- [ ] Every geometric requirement in the request is declared in `checks()` and passes — clear
|
|
35
|
-
regions for what must pass through or fit in, material regions for what must remain,
|
|
36
|
-
bbox bounds for stated size limits.
|
|
37
|
-
*Catches: a recess that swallowed its screw seat, a pocket the mating part cannot enter,
|
|
38
|
-
a beam corridor with a wall in it, a feature a fillet silently ate — all invisible to
|
|
39
|
-
`is_valid` and the bounding box.*
|
|
40
|
-
|
|
41
|
-
```bash
|
|
42
|
-
python scripts/check.py facts out/part.step
|
|
43
|
-
python scripts/check.py geometry out/part.step --model part_model.py
|
|
44
|
-
```
|
|
45
|
-
|
|
46
|
-
## 3. Interfaces
|
|
47
|
-
|
|
48
|
-
- [ ] Every interface dimension has a written source: a standard ID, a vendor drawing, or a user
|
|
49
|
-
measurement. **None came from memory.**
|
|
50
|
-
*Catches: the single most expensive failure mode in this skill.*
|
|
51
|
-
- [ ] Every interface covered by a standard is declared in the model's `interfaces()` and passes
|
|
52
|
-
`check.py interfaces`.
|
|
53
|
-
*Catches: an interface nobody checked because the outer bounding box could not see it.*
|
|
54
|
-
- [ ] Features that receive a standardised component use `intent: "envelope"`.
|
|
55
|
-
*Catches: a pocket sized to nominal, which fits only the smaller half of conforming parts.*
|
|
56
|
-
- [ ] Any standard entry marked `verified: false` was confirmed against the primary document, or
|
|
57
|
-
the user was told it is unconfirmed.
|
|
58
|
-
*Catches: propagating a derived number as if it were read from the standard.*
|
|
59
|
-
- [ ] Metric vs imperial is confirmed where both exist, and no expression mixes them.
|
|
60
|
-
*Catches: the 25.0 vs 25.4 mm grid error, which accumulates to 1.6 mm over four holes.*
|
|
61
|
-
- [ ] Interfaces not covered by any bundled standard — a vendor drawing, a measurement — were
|
|
62
|
-
reported to the user as unchecked, with the number and its source.
|
|
63
|
-
*Catches: a silent gap where the automatic check simply had nothing to say.*
|
|
64
|
-
|
|
65
|
-
```bash
|
|
66
|
-
python scripts/check.py interfaces out/part.manifest.json
|
|
67
|
-
|
|
68
|
-
# one dimension by hand, when it is not declared in the model
|
|
69
|
-
python scripts/check.py fit --standard <id> --intent envelope --clearance <mm> --value <dim>=<mm>
|
|
70
|
-
```
|
|
71
|
-
|
|
72
|
-
## 4. Fits and assembly
|
|
73
|
-
|
|
74
|
-
- [ ] Every mating dimension has a deliberate clearance chosen for the process.
|
|
75
|
-
*Catches: nominal-to-nominal fits, which do not assemble.*
|
|
76
|
-
- [ ] Multi-part assemblies were checked for interference.
|
|
77
|
-
*Catches: parts that overlap in CAD and therefore cannot exist together.*
|
|
78
|
-
- [ ] Rigid multi-hole mounting patterns have at least one slot.
|
|
79
|
-
*Catches: a four-hole bolt pattern binding on accumulated tolerance.*
|
|
80
|
-
|
|
81
|
-
```bash
|
|
82
|
-
python scripts/check.py clearance out/a.step out/b.step --min 0.3
|
|
83
|
-
```
|
|
84
|
-
|
|
85
|
-
## 5. Manufacturability
|
|
86
|
-
|
|
87
|
-
- [ ] Minimum wall and feature sizes are within the chosen process (`fabrication-limits.md`).
|
|
88
|
-
- [ ] Print or machining orientation is stated, and load runs along layers, not across them.
|
|
89
|
-
- [ ] Threads use inserts or captive nuts rather than printed threads, unless coarse.
|
|
90
|
-
- [ ] Enclosed cavities have a drain path for resin, and support-free access where possible.
|
|
91
|
-
- [ ] Milled internal corners have relief for the tool radius.
|
|
92
|
-
|
|
93
|
-
## 6. Material
|
|
94
|
-
|
|
95
|
-
- [ ] Material is compatible with the **cleaning agent**, not only the sample.
|
|
96
|
-
*Catches: acrylic crazing on 70% ethanol; PLA distorting in an autoclave.*
|
|
97
|
-
- [ ] Sterilisation method is stated and the material actually survives it.
|
|
98
|
-
- [ ] Anything contacting cells, tissue, or animals has a justified material, or contact is
|
|
99
|
-
designed out.
|
|
100
|
-
*Catches: assuming a printed resin part is cell-safe.*
|
|
101
|
-
- [ ] Optical requirements — autofluorescence, scatter, transmission — are addressed if the part is
|
|
102
|
-
near a beam or a detector.
|
|
103
|
-
|
|
104
|
-
## 7. Visual review — mandatory
|
|
105
|
-
|
|
106
|
-
- [ ] A snapshot was rendered **and read** after the most recent generation.
|
|
107
|
-
- [ ] Confirmed in the image: features on the intended faces; correct mold/chip polarity; every
|
|
108
|
-
port, bore, and boss present, inside the body, and passing through; nothing consumed by a
|
|
109
|
-
fillet; clear apertures unobstructed.
|
|
110
|
-
|
|
111
|
-
```bash
|
|
112
|
-
python scripts/snapshot.py out/part.step --out out/part.png
|
|
113
|
-
```
|
|
114
|
-
|
|
115
|
-
**This step is never waived by the numeric checks passing.** `is_valid: true` with a correct
|
|
116
|
-
bounding box is fully consistent with a pocket cut on the wrong face or an inverted mold. Those
|
|
117
|
-
errors are obvious in the picture and invisible in the numbers.
|
|
118
|
-
|
|
119
|
-
## 8. Report
|
|
120
|
-
|
|
121
|
-
Give the user, explicitly:
|
|
122
|
-
|
|
123
|
-
1. Process and material, and why.
|
|
124
|
-
2. Every interface dimension with its source and tolerance.
|
|
125
|
-
3. Clearances chosen, and the fit class they came from.
|
|
126
|
-
4. What the snapshot showed — described, not merely "a snapshot was generated".
|
|
127
|
-
5. Every check that did not pass, and every dimension you could not verify.
|
|
128
|
-
6. A recommendation to print a test coupon of the critical interface before committing to the full
|
|
129
|
-
part, whenever the design depends on a fit.
|
|
130
|
-
|
|
131
|
-
State the unverified items plainly. A part list with one honest "this dimension needs
|
|
132
|
-
confirmation" is far more useful than a confident one that is silently wrong.
|