wwood-bioruby 1.2.11

data/lib/bio/appl/blast/ddbj.rb

@@ -0,0 +1,142 @@
+#
+# = bio/appl/blast/ddbj.rb - Remote BLAST wrapper using DDBJ web service
+# 
+# Copyright::  Copyright (C) 2008       Naohisa Goto <ng@bioruby.org>
+# License::    The Ruby License
+#
+# $Id:$
+#
+
+require 'bio/appl/blast/remote'
+require 'bio/io/ddbjxml'
+
+module Bio::Blast::Remote
+
+  # Remote BLAST factory using DDBJ Web API for Biology
+  # (http://xml.nig.ac.jp/).
+  #
+  module DDBJ
+
+    # Creates a remote BLAST factory using DDBJ.
+    # Returns Bio::Blast object.
+    #
+    # Note for future improvement: In the future, it might return
+    # Bio::Blast::Remote::DDBJ or other object. 
+    #
+    def self.new(program, db, options = [])
+      Bio::Blast.new(program, db, options, 'ddbj')
+    end
+
+    # Information about DDBJ BLAST.
+    module Information
+
+      include Bio::Blast::Remote::Information
+
+      # (private) parse database information
+      def _parse_databases
+        if defined? @parse_databases
+          return nil if @parse_databases
+        end
+        drv = Bio::DDBJ::XML::Blast.new
+        str = drv.getSupportDatabaseList
+
+        databases = {}
+        dbdescs = {}
+        key = 'blastn'
+        prefix = ''
+        databases[key] ||= []
+        dbdescs[key] ||= {}
+        str.each_line do |line|
+          a = line.strip.split(/\s*\-\s*/, 2)
+          case a.size
+          when 1
+            prefix = a[0].to_s.strip
+            prefix += ': ' unless prefix.empty?
+            key = 'blastn'
+            next #each_line
+          when 0
+            prefix = ''
+            key = 'blastp'
+            databases[key] ||= []
+            dbdescs[key] ||= {}
+            next #each_line
+          end
+          name = a[0].to_s.strip.freeze
+          desc = (prefix + a[1].to_s.strip).freeze
+          databases[key].push name
+          dbdescs[key][name] = desc
+        end
+
+        databases['blastp'] ||= []
+        dbdescs['blastp'] ||= []
+
+        databases['blastn'].freeze
+        databases['blastp'].freeze
+
+        databases['blastx']  = databases['blastp']
+        dbdescs['blastx']    = dbdescs['blastp']
+        databases['tblastn'] = databases['blastn']
+        dbdescs['tblastn']   = dbdescs['blastn']
+        databases['tblastx'] = databases['blastn']
+        dbdescs['tblastx']   = dbdescs['blastn']
+
+        @databases = databases
+        @database_descriptions = dbdescs
+        @parse_databases = true
+        true
+      end
+      private :_parse_databases
+
+    end #module Information
+
+    extend Information
+
+    # executes BLAST and returns result as a string
+    def exec_ddbj(query)
+      options = make_command_line_options
+      opt = Bio::Blast::NCBIOptions.new(options)
+
+      # SOAP objects are cached
+      @ddbj_remote_blast ||= Bio::DDBJ::XML::Blast.new
+      #@ddbj_request_manager ||= Bio::DDBJ::XML::RequestManager.new
+      # always use REST version to prevent warning messages
+      @ddbj_request_manager ||= Bio::DDBJ::XML::RequestManager::REST.new
+
+      program = opt.delete('-p')
+      db = opt.delete('-d')
+      optstr = Bio::Command.make_command_line_unix(opt.options)
+
+      # using searchParamAsync 
+      qid = @ddbj_remote_blast.searchParamAsync(program, db, query, optstr)
+      @output = qid
+
+      sleeptime = 2
+      flag = true
+      while flag
+        if $VERBOSE then
+          $stderr.puts "DDBJ BLAST: ID: #{qid} -- waitng #{sleeptime} sec."
+        end
+        sleep(sleeptime)
+
+        result = @ddbj_request_manager.getAsyncResult(qid)
+        case result.to_s
+        when /The search and analysis service by WWW is very busy now/
+          raise result.to_s.strip + '(Alternatively, wrong options may be given.)'
+        when /Your job has not completed yet/
+          sleeptime = 5
+        else
+          flag = false
+        end
+      end while flag
+
+      @output = result
+      return @output
+    end
+
+  end #module DDBJ
+
+  # for lazy load DDBJ module
+  Ddbj = DDBJ
+
+end #module Bio::Blast::Remote
+

data/lib/bio/appl/blast/format0.rb

@@ -0,0 +1,1438 @@
+#
+# = bio/appl/blast/format0.rb - BLAST default output (-m 0) parser
+# 
+# Copyright::  Copyright (C) 2003-2006 GOTO Naohisa <ng@bioruby.org>
+# License::    The Ruby License
+#
+# $Id:$
+#
+# == Description
+#
+# NCBI BLAST default (-m 0 option) output parser.
+#
+# == References
+#
+# * Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
+#   Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
+#   "Gapped BLAST and PSI-BLAST: a new generation of protein database search
+#   programs", Nucleic Acids Res. 25:3389-3402.
+# * http://www.ncbi.nlm.nih.gov/blast/ 
+#
+
+begin
+  require 'strscan'
+rescue LoadError
+end
+require 'singleton'
+
+#--
+#require 'bio/db'
+#++
+require 'bio/io/flatfile'
+
+module Bio
+  class Blast
+    module Default #:nodoc:
+
+      # Bio::Blast::Default::Report parses NCBI BLAST default output
+      # and stores information in the data.
+      # It may store some Bio::Blast::Default::Report::Iteration objects.
+      class Report #< DB
+        # Delimiter of each entry. Bio::FlatFile uses it.
+        DELIMITER = RS = "\nBLAST"
+
+        # (Integer) excess read size included in DELIMITER.
+        DELIMITER_OVERRUN = 5 # "BLAST"
+
+        # Opens file by using Bio::FlatFile.open.
+        def self.open(filename, *mode)
+          Bio::FlatFile.open(self, filename, *mode)
+        end
+
+        # Creates a new Report object from BLAST result text.
+        def initialize(str)
+          str = str.sub(/\A\s+/, '')
+          str.sub!(/\n(T?BLAST.*)/m, "\n") # remove trailing entries for sure
+          @entry_overrun = $1
+          @entry = str
+          data = str.split(/(?:^[ \t]*\n)+/)
+
+          format0_split_headers(data)
+          @iterations = format0_split_search(data)
+          format0_split_stat_params(data)
+        end
+        # piece of next entry. Bio::FlatFile uses it.
+        attr_reader :entry_overrun
+
+        # (PSI-BLAST)
+        # Returns iterations.
+        # It returns an array of Bio::Blast::Default::Report::Iteration class.
+        # Note that normal blastall result usually contains one iteration.
+        attr_reader :iterations
+
+        # Returns whole entry as a string.
+        def to_s; @entry; end
+
+        #:stopdoc:
+        # prevent using StringScanner_R (in old version of strscan)
+        if !defined?(StringScanner) then
+          def initialize(*arg)
+            raise 'couldn\'t load strscan.so'
+          end #def
+        elsif StringScanner.name == 'StringScanner_R' then
+          def initialize(*arg)
+            raise 'cannot use StringScanner_R'
+          end #def
+        end
+        #:startdoc:
+
+        # Defines attributes which delegate to @f0dbstat objects.
+        def self.delegate_to_f0dbstat(*names)
+          names.each do |x|
+            module_eval("def #{x}; @f0dbstat.#{x}; end")
+          end
+        end
+        private_class_method :delegate_to_f0dbstat
+
+        # number of sequences in database
+        attr_reader          :db_num if false #dummy
+        delegate_to_f0dbstat :db_num
+
+        # number of letters in database
+        attr_reader          :db_len if false #dummy
+        delegate_to_f0dbstat :db_len
+
+        # posted date of the database
+        attr_reader          :posted_date if false #dummy
+        delegate_to_f0dbstat :posted_date
+
+        # effective length of the database
+        attr_reader          :eff_space if false #dummy
+        delegate_to_f0dbstat :eff_space
+
+        # name of the matrix
+        attr_reader          :matrix if false #dummy
+        delegate_to_f0dbstat :matrix
+
+        # match score of the matrix
+        attr_reader          :sc_match if false #dummy
+        delegate_to_f0dbstat :sc_match
+
+        # mismatch score of the matrix
+        attr_reader          :sc_mismatch if false #dummy
+        delegate_to_f0dbstat :sc_mismatch
+
+        # gap open penalty
+        attr_reader          :gap_open if false #dummy
+        delegate_to_f0dbstat :gap_open
+
+        # gap extend penalty
+        attr_reader          :gap_extend if false #dummy
+        delegate_to_f0dbstat :gap_extend
+
+        # e-value threshold specified when BLAST was executed
+        attr_reader          :expect if false #dummy
+        delegate_to_f0dbstat :expect
+
+        # number of hits. Note that this may differ from <tt>hits.size</tt>.
+        attr_reader          :num_hits if false #dummy
+        delegate_to_f0dbstat :num_hits
+
+        # Same as <tt>iterations.last.kappa</tt>.
+        def kappa;          @iterations.last.kappa;          end
+        # Same as <tt>iterations.last.lambda</tt>.
+        def lambda;         @iterations.last.lambda;         end
+        # Same as <tt>iterations.last.entropy</tt>.
+        def entropy;        @iterations.last.entropy;        end
+
+        # Same as <tt>iterations.last.gapped_kappa</tt>.
+        def gapped_kappa;   @iterations.last.gapped_kappa;   end
+        # Same as <tt>iterations.last.gapped_lambda</tt>.
+        def gapped_lambda;  @iterations.last.gapped_lambda;  end
+        # Same as <tt>iterations.last.gapped_entropy</tt>.
+        def gapped_entropy; @iterations.last.gapped_entropy; end
+
+        # Returns program name.
+        def program;        format0_parse_header; @program;        end
+        # Returns version of the program.
+        def version;        format0_parse_header; @version;        end
+        # Returns version number string of the program.
+        def version_number; format0_parse_header; @version_number; end
+        # Returns released date of the program.
+        def version_date;   format0_parse_header; @version_date;   end
+
+        # Returns length of the query.
+        def query_len; format0_parse_query; @query_len; end
+
+        # Returns definition of the query.
+        def query_def; format0_parse_query; @query_def; end
+
+        # (PHI-BLAST)
+        # Same as <tt>iterations.first.pattern</tt>.
+        # Note that it returns the FIRST iteration's value.
+        def pattern; @iterations.first.pattern; end
+
+        # (PHI-BLAST)
+        # Same as <tt>iterations.first.pattern_positions</tt>.
+        # Note that it returns the FIRST iteration's value.
+        def pattern_positions
+          @iterations.first.pattern_positions
+        end
+
+        # (PSI-BLAST)
+        # Iterates over each iteration.
+        # Same as <tt>iterations.each</tt>.
+        # Yields a Bio::Blast::Default::Report::Iteration object.
+        def each_iteration
+          @iterations.each do |x|
+            yield x
+          end
+        end
+
+        # Iterates over each hit of the last iteration.
+        # Same as <tt>iterations.last.each_hit</tt>.
+        # Yields a Bio::Blast::Default::Report::Hit object.
+        # This is very useful in most cases, e.g. for blastall results.
+        def each_hit
+          @iterations.last.each do |x|
+            yield x
+          end
+        end
+        alias each each_hit
+
+        # Same as <tt>iterations.last.hits</tt>.
+        # Returns the last iteration's hits.
+        # Returns an array of Bio::Blast::Default::Report::Hit object.
+        # This is very useful in most cases, e.g. for blastall results.
+        def hits
+          @iterations.last.hits
+        end
+
+        # (PSI-BLAST)
+        # Same as <tt>iterations.last.message</tt>.
+        def message
+          @iterations.last.message
+        end
+
+        # (PSI-BLAST)
+        # Same as <tt>iterations.last.converged?</tt>.
+        # Returns true if the last iteration is converged,
+        # otherwise, returns false.
+        def converged?
+          @iterations.last.converged?
+        end
+
+        # Returns the bibliography reference of the BLAST software. 
+        # Note that this method shows only the first reference.
+        # When you want to get additional references,
+        # you can use <tt>references</tt> method.
+        def reference
+          references[0]
+        end
+
+        # Returns the bibliography references of the BLAST software. 
+        # Returns an array of strings.
+        def references
+          unless defined?(@references)
+            @references = @f0references.collect do |x|
+              x.to_s.gsub(/\s+/, ' ').strip
+            end
+          end #unless
+          @references
+        end
+
+        # Returns the name (filename or title) of the database.
+        def db
+          unless defined?(@db)
+            if /Database *\: *(.*)/m =~ @f0database then
+              a = $1.split(/^/)
+              a.pop if a.size > 1
+              @db = a.collect { |x| x.sub(/\s+\z/, '') }.join(' ')
+            end
+          end #unless
+          @db
+        end
+
+        private
+        # Parses the query lines (begins with "Query = ").
+        def format0_parse_query
+          unless defined?(@query_def)
+            sc = StringScanner.new(@f0query)
+            sc.skip(/\s*/)
+            if sc.skip_until(/Query\= */) then
+              q = []
+              begin
+                q << sc.scan(/.*/)
+                sc.skip(/\s*^ ?/)
+              end until !sc.rest or r = sc.skip(/ *\( *([\,\d]+) *letters *\)\s*\z/)
+              @query_len = sc[1].delete(',').to_i if r
+              @query_def = q.join(' ')
+            end
+          end
+        end
+
+        # Parses the first line of the BLAST result.
+        def format0_parse_header
+          unless defined?(@program)
+            if /([\-\w]+) +([\w\-\.\d]+) *\[ *([\-\.\w]+) *\] *(\[.+\])?/ =~ @f0header.to_s
+              @program = $1
+              @version = "#{$1} #{$2} [#{$3}]"
+              @version_number = $2
+              @version_date = $3
+            end
+          end
+        end
+
+        # Splits headers into the first line, reference, query line and
+        # database line.
+        def format0_split_headers(data)
+          @f0header = data.shift
+          @f0references = []
+          while data[0] and /\AQuery\=/ !~ data[0]
+            @f0references.push data.shift
+          end
+          @f0query = data.shift
+          @f0database = data.shift
+          # In special case, a void line is inserted after database name.
+          if /\A +[\d\,]+ +sequences\; +[\d\,]+ total +letters\s*\z/ =~ data[0] then
+            @f0database.concat "\n"
+            @f0database.concat data.shift
+          end
+        end
+
+        # Splits the statistical parameters.
+        def format0_split_stat_params(data)
+          dbs = []
+          while r = data.first and /^ *Database\:/ =~ r
+            dbs << data.shift
+          end
+          @f0dbstat = self.class::F0dbstat.new(dbs)
+          i = -1
+          while r = data[0] and /^Lambda/ =~ r
+            #i -= 1 unless /^Gapped/ =~ r
+            if itr = @iterations[i] then
+              x = data.shift; itr.instance_eval { @f0stat << x }
+              x = @f0dbstat; itr.instance_eval { @f0dbstat = x }
+            end
+          end
+          @f0dbstat.f0params = data
+        end
+
+        # Splits the search results.
+        def format0_split_search(data)
+          iterations = []
+          while r = data[0] and /^Searching/ =~ r
+            iterations << Iteration.new(data)
+          end
+          iterations
+        end
+
+        # Stores format0 database statistics.
+        # Internal use only. Users must not use the class.
+        class F0dbstat #:nodoc:
+          # Creates new F0dbstat class.
+          # Internal use only.
+          def initialize(ary)
+            @f0dbstat = ary
+            @hash = {}
+          end
+          attr_reader :f0dbstat
+          attr_accessor :f0params
+
+          # Parses colon-separeted lines (in +ary+) and stores to +hash+.
+          def parse_colon_separated_params(hash, ary)
+            ary.each do |str|
+              sc = StringScanner.new(str)
+              sc.skip(/\s*/)
+              while sc.rest?
+                if sc.match?(/Number of sequences better than +([e\+\-\.\d]+) *\: *(.+)/) then
+                  ev = sc[1]
+                  ev = '1' + ev if ev[0] == ?e
+                  @expect = ev.to_f
+                  @num_hits = sc[2].tr(',', '').to_i
+                end
+                if sc.skip(/([\-\,\.\'\(\)\#\w ]+)\: *(.*)/) then
+                  hash[sc[1]] = sc[2]
+                else
+                  #p sc.peek(20)
+                  raise ScanError
+                end
+                sc.skip(/\s*/)
+              end #while
+            end #each
+          end #def
+          private :parse_colon_separated_params
+
+          # Parses parameters.
+          def parse_params
+            unless defined?(@parse_params)
+              parse_colon_separated_params(@hash, @f0params)
+              #p @hash
+              if val = @hash['Matrix'] then
+                if /blastn *matrix *\: *([e\+\-\.\d]+) +([e\+\-\.\d]+)/ =~ val then
+                  @matrix = 'blastn'
+                  @sc_match    = $1.to_i
+                  @sc_mismatch = $2.to_i 
+                else
+                  @matrix = val
+                end
+              end
+              if val = @hash['Gap Penalties'] then
+                if /Existence\: *([e\+\-\.\d]+)/ =~ val then
+                  @gap_open = $1.to_i
+                end
+                if /Extension\: *([e\+\-\.\d]+)/ =~ val then
+                  @gap_extend = $1.to_i
+                end
+              end
+              #@db_num = @hash['Number of Sequences'] unless defined?(@db_num)
+              #@db_len = @hash['length of database']  unless defined?(@db_len)
+              if val = @hash['effective search space'] then
+                @eff_space = val.tr(',', '').to_i
+              end
+              @parse_params = true
+            end #unless
+          end
+          private :parse_params
+
+          # Returns name of the matrix.
+          def matrix;      parse_params; @matrix;      end
+          # Returns the match score of the matrix.
+          def sc_match;    parse_params; @sc_match;    end
+          # Returns the mismatch score of the matrix.
+          def sc_mismatch; parse_params; @sc_mismatch; end
+
+          # Returns gap open penalty value.
+          def gap_open;    parse_params; @gap_open;    end
+          # Returns gap extend penalty value.
+          def gap_extend;  parse_params; @gap_extend;  end
+
+          # Returns effective length of the database.
+          def eff_space;   parse_params; @eff_space;   end
+
+          # Returns e-value threshold specified when BLAST was executed.
+          def expect;      parse_params; @expect;      end
+
+          # Returns number of hits.
+          def num_hits;    parse_params; @num_hits;    end
+
+          # Parses database statistics lines.
+          def parse_dbstat
+            a = @f0dbstat[0].to_s.split(/^/)
+            d = []
+            i = 3
+            while i > 0 and line = a.pop
+              case line
+              when /^\s+Posted date\:\s*(.*)$/
+                unless defined?(@posted_date)
+                  @posted_date = $1.strip
+                  i -= 1; d.clear
+                end
+              when /^\s+Number of letters in database\:\s*(.*)$/
+                unless defined?(@db_len)
+                  @db_len =  $1.tr(',', '').to_i
+                  i -= 1; d.clear
+                end
+              when /^\s+Number of sequences in database\:\s*(.*)$/
+                unless defined?(@db_num)
+                  @db_num = $1.tr(',', '').to_i
+                  i -= 1; d.clear
+                end
+              else
+                d.unshift(line)
+              end
+            end #while
+            a.concat(d)
+            while line = a.shift
+              if /^\s+Database\:\s*(.*)$/ =~ line
+                a.unshift($1)
+                a.each { |x| x.strip! }
+                @database = a.join(' ')
+                break #while
+              end
+            end
+          end #def
+          private :parse_dbstat
+
+          # Returns name (title or filename) of the database.
+          def database
+            unless defined?(@database);    parse_dbstat; end; @database
+          end
+
+          # Returns posted date of the database.
+          def posted_date
+            unless defined?(@posted_date); parse_dbstat; end; @posted_date
+          end
+
+          # Returns number of letters in database.
+          def db_len
+            unless defined?(@db_len);      parse_dbstat; end; @db_len
+          end
+
+          # Returns number of sequences in database.
+          def db_num
+            unless defined?(@db_num);      parse_dbstat; end; @db_num
+          end
+        end #class F0dbstat
+
+        # Provides a singleton object of which any methods always return nil.
+        # Internal use only. Users must not use the class.
+        class AlwaysNil #:nodoc:
+          include Singleton
+          def method_missing(*arg)
+            nil
+          end
+        end #class AlwaysNil
+
+        # Bio::Blast::Default::Report::Iteration stores information about
+        # a iteration.
+        # It may contain some Bio::Blast::Default::Report::Hit objects.
+        # Note that a PSI-BLAST (blastpgp command) result usually contain
+        # multiple iterations in it, and a normal BLAST (blastall command)
+        # result usually contain one iteration in it.
+        class Iteration
+          # Creates a new Iteration object.
+          # It is designed to be called only internally from
+          # the Bio::Blast::Default::Report class.
+          # Users shall not use the method directly.
+          def initialize(data)
+            @f0stat = []
+            @f0dbstat = AlwaysNil.instance
+            @f0hitlist = []
+            @hits = []
+            @num = 1
+            r = data.shift
+            @f0message = [ r ]
+            r.gsub!(/^Results from round (\d+).*\z/) { |x|
+              @num = $1.to_i
+              @f0message << x
+              ''
+            }
+            r = data.shift
+            while /^Number of occurrences of pattern in the database is +(\d+)/ =~ r
+              # PHI-BLAST
+              @pattern_in_database = $1.to_i
+              @f0message << r
+              r = data.shift
+            end
+            if /^Results from round (\d+)/ =~ r then
+              @num = $1.to_i
+              @f0message << r
+              r = data.shift
+            end
+            if r and !(/\*{5} No hits found \*{5}/ =~ r) then
+              @f0hitlist << r
+              begin
+                @f0hitlist << data.shift
+              end until r = data[0] and /^\>/ =~ r
+              if r and /^CONVERGED\!/ =~ r then
+                r.sub!(/(.*\n)*^CONVERGED\!.*\n/) { |x| @f0hitlist << x; '' }
+              end
+              if defined?(@pattern_in_database) and r = data.first then
+                #PHI-BLAST
+                while /^\>/ =~ r
+                  @hits << Hit.new(data)
+                  r = data.first
+                  break unless r
+                  while /^Significant alignments for pattern/ =~ r
+                    data.shift
+                    r = data.first
+                  end
+                end
+              else
+                #not PHI-BLAST
+                while r = data[0] and /^\>/ =~ r
+                  @hits << Hit.new(data)
+                end
+              end
+            end
+            if /^CONVERGED\!\s*$/ =~ @f0hitlist[-1].to_s then
+              @message = 'CONVERGED!'
+              @flag_converged = true
+            end
+          end
+
+          # (PSI-BLAST) Iteration round number.
+          attr_reader :num
+          # (PSI-BLAST) Messages of the iteration.
+          attr_reader :message
+          # (PHI-BLAST) Number of occurrences of pattern in the database.
+          attr_reader :pattern_in_database
+
+          # Returns the hits of the iteration.
+          # It returns an array of Bio::Blast::Default::Report::Hit objects.
+          def hits
+            parse_hitlist
+            @hits
+          end
+
+          # Iterates over each hit of the iteration.
+          # Yields a Bio::Blast::Default::Report::Hit object.
+          def each
+            hits.each do |x|
+              yield x
+            end
+          end
+
+          # (PSI-BLAST) Returns true if the iteration is converged.
+          # Otherwise, returns false.
+          def converged?
+            @flag_converged
+          end
+
+          # (PHI-BLAST) Returns pattern string.
+          # Returns nil if it is not a PHI-BLAST result.
+          def pattern
+            #PHI-BLAST
+            if !defined?(@pattern) and defined?(@pattern_in_database) then
+              @pattern = nil
+              @pattern_positions = []
+              @f0message.each do |r|
+                sc = StringScanner.new(r)
+                if sc.skip_until(/^ *pattern +([^\s]+)/) then
+                  @pattern = sc[1] unless @pattern
+                  sc.skip_until(/(?:^ *| +)at position +(\d+) +of +query +sequence/)
+                  @pattern_positions << sc[1].to_i
+                end
+              end
+            end
+            @pattern
+          end
+
+          # (PHI-BLAST) Returns pattern positions.
+          # Returns nil if it is not a PHI-BLAST result.
+          def pattern_positions
+            #PHI-BLAST
+            pattern
+            @pattern_positions
+          end
+
+          # (PSI-BLAST)
+          # Returns hits which have been found again in the iteration.
+          # It returns an array of Bio::Blast::Default::Report::Hit objects.
+          def hits_found_again
+            parse_hitlist
+            @hits_found_again
+          end
+
+          # (PSI-BLAST)
+          # Returns hits which have been newly found in the iteration.
+          # It returns an array of Bio::Blast::Default::Report::Hit objects.
+          def hits_newly_found
+            parse_hitlist
+            @hits_newly_found
+          end
+
+          # (PHI-BLAST) Returns hits for pattern. ????
+          def hits_for_pattern
+            parse_hitlist
+            @hits_for_pattern
+          end
+
+          # Parses list of hits.
+          def parse_hitlist
+            unless defined?(@parse_hitlist)
+              @hits_found_again = []
+              @hits_newly_found = []
+              @hits_unknown_state = []
+              i = 0
+              a = @hits_newly_found
+              flag = true
+              @f0hitlist.each do |x|
+                sc = StringScanner.new(x)
+                if flag then
+                  if sc.skip_until(/^Sequences used in model and found again\:\s*$/)
+                    a = @hits_found_again
+                  end
+                  flag = nil
+                  next
+                end
+                next if sc.skip(/^CONVERGED\!$/)
+                if sc.skip(/^Sequences not found previously or not previously below threshold\:\s*$/) then
+                  a = @hits_newly_found
+                  next
+                elsif sc.skip(/^Sequences.+\:\s*$/) then
+                  #possibly a bug or unknown format?
+                  a = @hits_unknown_state
+                  next
+                elsif sc.skip(/^Significant (matches|alignments) for pattern/) then
+                  # PHI-BLAST
+                  # do nothing when 'alignments'
+                  if sc[1] == 'matches' then
+                    unless defined?(@hits_for_pattern)
+                      @hits_for_pattern = []
+                    end
+                    a = []
+                    @hits_for_pattern << a
+                  end
+                  next
+                end
+                b = x.split(/^/)
+                b.collect! { |y| y.empty? ? nil : y }
+                b.compact!
+                if i + b.size > @hits.size then
+                  ((@hits.size - i)...(b.size)).each do |j|
+                    y = b[j]; y.strip!
+                    y.reverse!
+                    z = y.split(/\s+/, 3)
+                    z.each { |y| y.reverse! }
+                    h = Hit.new([ z.pop.to_s.sub(/\.+\z/, '') ])
+                    bs = z.pop.to_s
+                    bs = '1' + bs if bs[0] == ?e
+                    bs = (bs.empty? ? nil : bs.to_f)
+                    ev = z.pop.to_s
+                    ev = '1' + ev if ev[0] == ?e
+                    ev = (ev.empty? ? (1.0/0.0) : ev.to_f)
+                    h.instance_eval { @bit_score = bs; @evalue = ev }
+                    @hits << h
+                  end
+                end
+                a.concat(@hits[i, b.size])
+                i += b.size
+              end #each
+              @hits_found_again.each do |x|
+                x.instance_eval { @again = true }
+              end
+              @parse_hitlist = true
+            end #unless
+          end
+          private :parse_hitlist
+
+          # Parses statistics for the iteration.
+          def parse_stat
+            unless defined?(@parse_stat)
+              @f0stat.each do |x|
+                gapped = nil
+                sc = StringScanner.new(x)
+                sc.skip(/\s*/)
+                if sc.skip(/Gapped\s*/) then
+                  gapped = true
+                end
+                s0 = []
+                h = {}
+                while r = sc.scan(/\w+/)
+                  #p r
+                  s0 << r
+                  sc.skip(/ */)
+                end
+                sc.skip(/\s*/)
+                while r = sc.scan(/[e\+\-\.\d]+/)
+                  #p r
+                  h[s0.shift] = r
+                  sc.skip(/ */)
+                end
+                if gapped then
+                  @gapped_lambda = (v = h['Lambda']) ? v.to_f : nil
+                  @gapped_kappa = (v = h['K']) ? v.to_f : nil
+                  @gapped_entropy = (v = h['H']) ? v.to_f : nil
+                else
+                  @lambda = (v = h['Lambda']) ? v.to_f : nil
+                  @kappa = (v = h['K']) ? v.to_f : nil
+                  @entropy = (v = h['H']) ? v.to_f : nil
+                end
+              end #each
+              @parse_stat = true
+            end #unless
+          end #def
+          private :parse_stat
+
+          # Defines attributes which call +parse_stat+ before accessing.
+          def self.method_after_parse_stat(*names)
+            names.each do |x|
+              module_eval("def #{x}; parse_stat; @#{x}; end")
+            end
+          end
+          private_class_method :method_after_parse_stat
+
+          # lambda of the database
+          attr_reader             :lambda  if false #dummy
+          method_after_parse_stat :lambda
+          # kappa of the database
+          attr_reader             :kappa   if false #dummy
+          method_after_parse_stat :kappa
+          # entropy of the database
+          attr_reader             :entropy if false #dummy
+          method_after_parse_stat :entropy
+
+          # gapped lambda of the database
+          attr_reader             :gapped_lambda  if false #dummy
+          method_after_parse_stat :gapped_lambda
+          # gapped kappa of the database
+          attr_reader             :gapped_kappa   if false #dummy
+          method_after_parse_stat :gapped_kappa
+          # gapped entropy of the database
+          attr_reader             :gapped_entropy if false #dummy
+          method_after_parse_stat :gapped_entropy
+
+          # Defines attributes which delegate to @f0dbstat objects.
+          def self.delegate_to_f0dbstat(*names)
+            names.each do |x|
+              module_eval("def #{x}; @f0dbstat.#{x}; end")
+            end
+          end
+          private_class_method :delegate_to_f0dbstat
+
+          # name (title or filename) of the database
+          attr_reader          :database if false #dummy
+          delegate_to_f0dbstat :database
+          # posted date of the database
+          attr_reader          :posted_date if false #dummy
+          delegate_to_f0dbstat :posted_date
+
+          # number of letters in database
+          attr_reader          :db_num if false #dummy
+          delegate_to_f0dbstat :db_num
+          # number of sequences in database
+          attr_reader          :db_len if false #dummy
+          delegate_to_f0dbstat :db_len
+          # effective length of the database
+          attr_reader          :eff_space if false #dummy
+          delegate_to_f0dbstat :eff_space
+
+          # e-value threshold specified when BLAST was executed
+          attr_reader          :expect if false #dummy
+          delegate_to_f0dbstat :expect
+
+        end #class Iteration
+
+        # Bio::Blast::Default::Report::Hit contains information about a hit.
+        # It may contain some Bio::Blast::Default::Report::HSP objects.
+        class Hit
+          # Creates a new Hit object.
+          # It is designed to be called only internally from the
+          # Bio::Blast::Default::Report::Iteration class.
+          # Users should not call the method directly.
+          def initialize(data)
+            @f0hitname = data.shift
+            @hsps = []
+            while r = data[0] and /\A\s+Score/ =~ r
+              @hsps << HSP.new(data)
+            end
+            @again = false
+          end
+
+          # Hsp(high-scoring segment pair)s of the hit.
+          # Returns an array of Bio::Blast::Default::Report::HSP objects.
+          attr_reader :hsps
+
+          # Iterates over each hsp(high-scoring segment pair) of the hit.
+          # Yields a Bio::Blast::Default::Report::HSP object.
+          def each
+            @hsps.each { |x| yield x }
+          end
+
+          # (PSI-BLAST)
+          # Returns true if the hit is found again in the iteration.
+          # Otherwise, returns false or nil.
+          def found_again?
+            @again
+          end
+
+          # Returns first hsp's score.
+          def score
+            (h = @hsps.first) ? h.score : nil
+          end
+
+          # Returns first hsp's bit score.
+          # (shown in hit list of BLAST result)
+          def bit_score
+            unless defined?(@bit_score)
+              if h = @hsps.first then
+                @bit_score = h.bit_score
+              end
+            end
+            @bit_score
+          end
+
+          # Returns first hsp's e-value.
+          # (shown in hit list of BLAST result)
+          def evalue
+            unless defined?(@evalue)
+              if h = @hsps.first then
+                @evalue = h.evalue
+              end
+            end
+            @evalue
+          end
+
+          # Parses name of the hit.
+          def parse_hitname
+            unless defined?(@parse_hitname)
+              sc = StringScanner.new(@f0hitname)
+              sc.skip(/\s*/)
+              sc.skip(/\>/)
+              d = []
+              begin
+                d << sc.scan(/.*/)
+                sc.skip(/\s*/)
+              end until !sc.rest? or r = sc.skip(/ *Length *\= *([\,\d]+)\s*\z/)
+              @len = (r ? sc[1].to_i : nil)
+              @definition = d.join(" ")
+              @parse_hitname = true
+            end
+          end
+          private :parse_hitname
+
+          # Returns length of the hit.
+          def len;        parse_hitname; @len;        end
+
+          # Returns definition of the hit.
+          def definition; parse_hitname; @definition; end
+
+          def target_id; definition[/^\s*(\S+)/, 1]; end
+
+          #--
+          # Aliases to keep compatibility with Bio::Fasta::Report::Hit.
+          alias target_def definition
+          alias target_len len
+          #++
+
+          # Sends given method to the first hsp or returns nil if
+          # there are no hsps.
+          def hsp_first(m)
+            (h = hsps.first) ? h.send(m) : nil
+          end
+          private :hsp_first
+
+          #--
+          # Shortcut methods for the best Hsp
+          # (Compatibility method with FASTA)
+          #++
+
+          # Same as hsps.first.identity.
+          # Returns nil if there are no hsp in the hit.
+          # (Compatibility method with FASTA)
+          def identity;      hsp_first :identity;     end
+
+          # Same as hsps.first.align_len.
+          # Returns nil if there are no hsp in the hit.
+          # (Compatibility method with FASTA)
+          def overlap;       hsp_first :align_len;    end
+
+          # Same as hsps.first.qseq.
+          # Returns nil if there are no hsp in the hit.
+          # (Compatibility method with FASTA)
+          def query_seq;     hsp_first :qseq;         end
+
+          # Same as hsps.first.hseq.
+          # Returns nil if there are no hsp in the hit.
+          # (Compatibility method with FASTA)
+          def target_seq;    hsp_first :hseq;         end
+
+          # Same as hsps.first.midline.
+          # Returns nil if there are no hsp in the hit.
+          # (Compatibility method with FASTA)
+          def midline;       hsp_first :midline;      end
+
+          # Same as hsps.first.query_from.
+          # Returns nil if there are no hsp in the hit.
+          # (Compatibility method with FASTA)
+          def query_start;   hsp_first :query_from;   end
+
+          # Same as hsps.first.query_to.
+          # Returns nil if there are no hsp in the hit.
+          # (Compatibility method with FASTA)
+          def query_end;     hsp_first :query_to;     end
+
+          # Same as hsps.first.hit_from.
+          # Returns nil if there are no hsp in the hit.
+          # (Compatibility method with FASTA)
+          def target_start;  hsp_first :hit_from;     end
+
+          # Same as hsps.first.hit_to.
+          # Returns nil if there are no hsp in the hit.
+          # (Compatibility method with FASTA)
+          def target_end;    hsp_first :hit_to;       end
+
+          # Returns an array which contains
+          # [ query_start, query_end, target_start, target_end ].
+          # (Compatibility method with FASTA)
+          def lap_at
+            [ query_start, query_end, target_start, target_end ]
+          end
+        end #class Hit
+
+        # Bio::Blast::Default::Report::HSP holds information about the hsp
+        # (high-scoring segment pair).
+        class HSP
+          # Creates new HSP object.
+          # It is designed to be called only internally from the
+          # Bio::Blast::Default::Report::Hit class.
+          # Users should not call the method directly.
+          def initialize(data)
+            @f0score = data.shift
+            @f0alignment = []
+            while r = data[0] and /^(Query|Sbjct)\:/ =~ r
+              @f0alignment << data.shift
+            end
+          end
+
+          # Parses scores, identities, positives, gaps, and so on.
+          def parse_score
+            unless defined?(@parse_score)
+              sc = StringScanner.new(@f0score)
+              while sc.rest?
+                sc.skip(/\s*/)
+                if sc.skip(/Expect(?:\(\d+\))? *\= *([e\+\-\.\d]+)/) then
+                  ev = sc[1].to_s
+                  ev = '1' + ev if ev[0] == ?e
+                  @evalue = ev.to_f
+                elsif sc.skip(/Score *\= *([e\+\-\.\d]+) *bits *\( *([e\+\-\.\d]+) *\)/) then
+                  bs = sc[1]
+                  bs = '1' + bs if bs[0] == ?e
+                  @bit_score = bs.to_f
+                  @score = sc[2].to_i
+                elsif sc.skip(/(Identities|Positives|Gaps) *\= (\d+) *\/ *(\d+) *\(([\.\d]+) *\% *\)/) then
+                  alen = sc[3].to_i
+                  @align_len = alen unless defined?(@align_len)
+                  raise ScanError if alen != @align_len
+                  case sc[1]
+                  when 'Identities'
+                    @identity = sc[2].to_i
+                    @percent_identity = sc[4].to_i
+                  when 'Positives'
+                    @positive = sc[2].to_i
+                    @percent_positive = sc[4].to_i
+                  when 'Gaps'
+                    @gaps = sc[2].to_i
+                    @percent_gaps = sc[4].to_i
+                  else
+                    raise ScanError
+                  end
+                elsif sc.skip(/Strand *\= *(Plus|Minus) *\/ *(Plus|Minus)/) then
+                  @query_strand = sc[1]
+                  @hit_strand = sc[2]
+                  if sc[1] == sc[2] then
+                    @query_frame = 1
+                    @hit_frame = 1
+                  elsif sc[1] == 'Plus' then # Plus/Minus
+                    # complement sequence against xml(-m 7)
+                    # In xml(-m 8), -1=>Plus, 1=>Minus ???
+                    #@query_frame = -1
+                    #@hit_frame = 1
+                    @query_frame = 1
+                    @hit_frame = -1
+                  else # Minus/Plus
+                    @query_frame = -1
+                    @hit_frame = 1
+                  end
+                elsif sc.skip(/Frame *\= *([\-\+]\d+)( *\/ *([\-\+]\d+))?/) then
+                  @query_frame = sc[1].to_i
+                  if sc[2] then
+                    @hit_frame = sc[3].to_i
+                  end
+                elsif sc.skip(/Score *\= *([e\+\-\.\d]+) +\(([e\+\-\.\d]+) *bits *\)/) then
+                  #WU-BLAST
+                  @score = sc[1].to_i
+                  bs = sc[2]
+                  bs = '1' + bs if bs[0] == ?e
+                  @bit_score = bs.to_f
+                elsif sc.skip(/P *\= * ([e\+\-\.\d]+)/) then
+                  #WU-BLAST
+                  @p_sum_n = nil
+                  pv = sc[1]
+                  pv = '1' + pv if pv[0] == ?e
+                  @pvalue = pv.to_f
+                elsif sc.skip(/Sum +P *\( *(\d+) *\) *\= *([e\+\-\.\d]+)/) then
+                  #WU-BLAST
+                  @p_sum_n = sc[1].to_i
+                  pv = sc[2]
+                  pv = '1' + pv if pv[0] == ?e
+                  @pvalue = pv.to_f
+                elsif sc.skip(/Method\:\s*(.+)/) then
+                  # signature of composition-based statistics method
+                  # for example, "Method: Composition-based stats."
+                  @stat_method = sc[1]
+                else
+                  raise ScanError
+                end
+                sc.skip(/\s*\,?\s*/)
+              end
+              @parse_score = true
+            end
+          end
+          private :parse_score
+
+          # Defines attributes which call parse_score before accessing.
+          def self.method_after_parse_score(*names)
+            names.each do |x|
+              module_eval("def #{x}; parse_score; @#{x}; end")
+            end
+          end
+          private_class_method :method_after_parse_score
+
+          # bit score
+          attr_reader              :bit_score if false #dummy
+          method_after_parse_score :bit_score
+          # score
+          attr_reader              :score if false #dummy
+          method_after_parse_score :score
+
+          # e-value
+          attr_reader              :evalue if false #dummy
+          method_after_parse_score :evalue
+
+          # frame of the query
+          attr_reader              :query_frame if false #dummy
+          method_after_parse_score :query_frame
+          # frame of the hit
+          attr_reader              :hit_frame if false #dummy
+          method_after_parse_score :hit_frame
+
+          # Identity (number of identical nucleotides or amino acids)
+          attr_reader              :identity if false #dummy
+          method_after_parse_score :identity
+          # percent of identical nucleotides or amino acids
+          attr_reader              :percent_identity if false #dummy
+          method_after_parse_score :percent_identity
+
+          # Positives (number of positive hit amino acids or nucleotides)
+          attr_reader              :positive if false #dummy
+          method_after_parse_score :positive
+          # percent of positive hit amino acids or nucleotides
+          attr_reader              :percent_positive if false #dummy
+          method_after_parse_score :percent_positive
+
+          # Gaps (number of gaps)
+          attr_reader              :gaps if false #dummy
+          method_after_parse_score :gaps
+          # percent of gaps
+          attr_reader              :percent_gaps if false #dummy
+          method_after_parse_score :percent_gaps
+
+          # aligned length
+          attr_reader              :align_len if false #dummy
+          method_after_parse_score :align_len
+
+          # strand of the query ("Plus" or "Minus" or nil)
+          attr_reader              :query_strand if false #dummy
+          method_after_parse_score :query_strand
+
+          # strand of the hit ("Plus" or "Minus" or nil)
+          attr_reader              :hit_strand if false #dummy
+          method_after_parse_score :hit_strand
+
+          # statistical method for calculating evalue and/or score
+          # (nil or a string)
+          # (note that composition-based statistics for blastp or tblastn
+          # were enabled by default after NCBI BLAST 2.2.17)
+          attr_reader              :stat_method if false #dummy
+          method_after_parse_score :stat_method
+
+          # Parses alignments.
+          def parse_alignment
+            unless defined?(@parse_alignment)
+              qpos1 = nil
+              qpos2 = nil
+              spos1 = nil
+              spos2 = nil
+              qseq = []
+              sseq = []
+              mseq = []
+              pos_st = nil
+              len_seq = 0
+              nextline = :q
+              @f0alignment.each do |x|
+                sc = StringScanner.new(x)
+                while sc.rest?
+                  #p pos_st, len_seq
+                  #p nextline.to_s
+                  if r = sc.skip(/(Query|Sbjct)\: *(\d+) */) then
+                    pos_st = r
+                    qs = sc[1]
+                    pos1 = sc[2]
+                    len_seq = sc.skip(/[^ ]*/)
+                    seq = sc[0]
+                    sc.skip(/ *(\d+) *\n/)
+                    pos2 = sc[1]
+                    if qs == 'Query' then
+                      raise ScanError unless nextline == :q
+                      qpos1 = pos1.to_i unless qpos1
+                      qpos2 = pos2.to_i
+                      qseq << seq
+                      nextline = :m
+                    elsif qs == 'Sbjct' then
+                      if nextline == :m then
+                        mseq << (' ' * len_seq)
+                      end
+                      spos1 = pos1.to_i unless spos1
+                      spos2 = pos2.to_i
+                      sseq << seq
+                      nextline = :q
+                    else
+                      raise ScanError
+                    end
+                  elsif r = sc.scan(/ {6}.+/) then
+                    raise ScanError unless nextline == :m
+                    mseq << r[pos_st, len_seq]
+                    sc.skip(/\n/)
+                    nextline = :s
+                  elsif r = sc.skip(/pattern +\d+.+/) then
+                    # PHI-BLAST
+                    # do nothing
+                    sc.skip(/\n/)
+                  else
+                    raise ScanError
+                  end
+                end #while
+              end #each
+              #p qseq, sseq, mseq
+              @qseq = qseq.join('')
+              @hseq = sseq.join('')
+              @midline = mseq.join('')
+              @query_from = qpos1
+              @query_to   = qpos2
+              @hit_from = spos1
+              @hit_to   = spos2
+              @parse_alignment = true
+            end #unless
+          end #def
+          private :parse_alignment
+
+          # Defines attributes which call parse_alignment before accessing.
+          def self.method_after_parse_alignment(*names)
+            names.each do |x|
+              module_eval("def #{x}; parse_alignment; @#{x}; end")
+            end
+          end
+          private_class_method :method_after_parse_alignment
+
+          # query sequence (with gaps) of the alignment of the hsp
+          attr_reader                  :qseq if false #dummy
+          method_after_parse_alignment :qseq
+          # hit sequence (with gaps) of the alignment of the hsp
+          attr_reader                  :hseq if false #dummy
+          method_after_parse_alignment :hseq
+
+          # middle line of the alignment of the hsp
+          attr_reader                  :midline if false #dummy
+          method_after_parse_alignment :midline
+
+          # start position of the query (the first position is 1)
+          attr_reader                  :query_from if false #dummy
+          method_after_parse_alignment :query_from
+
+          # end position of the query (including its position)
+          attr_reader                  :query_to
+          method_after_parse_alignment :query_to
+
+          # start position of the hit (the first position is 1)
+          attr_reader                  :hit_from if false #dummy
+          method_after_parse_alignment :hit_from
+
+          # end position of the hit (including its position)
+          attr_reader                  :hit_to if false #dummy
+          method_after_parse_alignment :hit_to
+
+        end #class HSP
+
+      end #class Report
+
+      # NCBI BLAST default (-m 0 option) output parser for TBLAST.
+      # All methods are equal to Bio::Blast::Default::Report.
+      # Only DELIMITER (and RS) is different.
+      class Report_TBlast < Report
+        # Delimter of each entry for TBLAST. Bio::FlatFile uses it.
+        DELIMITER = RS = "\nTBLAST"
+
+        # (Integer) excess read size included in DELIMITER.
+        DELIMITER_OVERRUN = 6 # "TBLAST"
+      end #class Report_TBlast
+
+    end #module Default
+  end #class Blast
+end #module Bio
+
+######################################################################
+
+if __FILE__ == $0
+
+  Bio::FlatFile.open(Bio::Blast::Default::Report, ARGF) do |ff|
+  ff.each do |rep|
+
+  print "# === Bio::Blast::Default::Report\n"
+  puts
+  print "  rep.program           #=> "; p rep.program
+  print "  rep.version           #=> "; p rep.version
+  print "  rep.reference         #=> "; p rep.reference
+  print "  rep.db                #=> "; p rep.db
+  #print "  rep.query_id          #=> "; p rep.query_id
+  print "  rep.query_def         #=> "; p rep.query_def
+  print "  rep.query_len         #=> "; p rep.query_len
+  #puts
+  print "  rep.version_number    #=> "; p rep.version_number
+  print "  rep.version_date      #=> "; p rep.version_date
+  puts
+
+  print "# === Parameters\n"
+  #puts
+  #print "  rep.parameters        #=> "; p rep.parameters
+  puts
+  print "  rep.matrix            #=> "; p rep.matrix
+  print "  rep.expect            #=> "; p rep.expect
+  #print "  rep.inclusion         #=> "; p rep.inclusion
+  print "  rep.sc_match          #=> "; p rep.sc_match
+  print "  rep.sc_mismatch       #=> "; p rep.sc_mismatch
+  print "  rep.gap_open          #=> "; p rep.gap_open
+  print "  rep.gap_extend        #=> "; p rep.gap_extend
+  #print "  rep.filter            #=> "; p rep.filter
+  print "  rep.pattern           #=> "; p rep.pattern
+  #print "  rep.entrez_query      #=> "; p rep.entrez_query
+  #puts
+  print "  rep.pattern_positions  #=> "; p rep.pattern_positions
+  puts
+
+  print "# === Statistics (last iteration's)\n"
+  #puts
+  #print "  rep.statistics        #=> "; p rep.statistics
+  puts
+  print "  rep.db_num            #=> "; p rep.db_num
+  print "  rep.db_len            #=> "; p rep.db_len
+  #print "  rep.hsp_len           #=> "; p rep.hsp_len
+  print "  rep.eff_space         #=> "; p rep.eff_space
+  print "  rep.kappa             #=> "; p rep.kappa
+  print "  rep.lambda            #=> "; p rep.lambda
+  print "  rep.entropy           #=> "; p rep.entropy
+  puts
+  print "  rep.num_hits          #=> "; p rep.num_hits
+  print "  rep.gapped_kappa      #=> "; p rep.gapped_kappa
+  print "  rep.gapped_lambda     #=> "; p rep.gapped_lambda
+  print "  rep.gapped_entropy    #=> "; p rep.gapped_entropy
+  print "  rep.posted_date       #=> "; p rep.posted_date
+  puts
+
+  print "# === Message (last iteration's)\n"
+  puts
+  print "  rep.message           #=> "; p rep.message
+  #puts
+  print "  rep.converged?        #=> "; p rep.converged?
+  puts
+
+  print "# === Iterations\n"
+  puts
+  print "  rep.itrerations.each do |itr|\n"
+  puts
+
+  rep.iterations.each do |itr|
+      
+  print "# --- Bio::Blast::Default::Report::Iteration\n"
+  puts
+
+  print "    itr.num             #=> "; p itr.num
+  #print "    itr.statistics      #=> "; p itr.statistics
+  print "    itr.message         #=> "; p itr.message
+  print "    itr.hits.size       #=> "; p itr.hits.size
+  #puts
+  print "    itr.hits_newly_found.size    #=> "; p itr.hits_newly_found.size;
+  print "    itr.hits_found_again.size    #=> "; p itr.hits_found_again.size;
+  if itr.hits_for_pattern then
+  itr.hits_for_pattern.each_with_index do |hp, hpi|
+  print "    itr.hits_for_pattern[#{hpi}].size #=> "; p hp.size;
+  end
+  end
+  print "    itr.converged?      #=> "; p itr.converged?
+  puts
+
+  print "    itr.hits.each do |hit|\n"
+  puts
+
+  itr.hits.each_with_index do |hit, i|
+
+  print "# --- Bio::Blast::Default::Report::Hit"
+  print " ([#{i}])\n"
+  puts
+
+  #print "      hit.num           #=> "; p hit.num
+  #print "      hit.hit_id        #=> "; p hit.hit_id
+  print "      hit.len           #=> "; p hit.len
+  print "      hit.definition    #=> "; p hit.definition
+  #print "      hit.accession     #=> "; p hit.accession
+  #puts
+  print "      hit.found_again?  #=> "; p hit.found_again?
+
+  print "        --- compatible/shortcut ---\n"
+  #print "      hit.query_id      #=> "; p hit.query_id
+  #print "      hit.query_def     #=> "; p hit.query_def
+  #print "      hit.query_len     #=> "; p hit.query_len
+  #print "      hit.target_id     #=> "; p hit.target_id
+  print "      hit.target_def    #=> "; p hit.target_def
+  print "      hit.target_len    #=> "; p hit.target_len
+
+  print "            --- first HSP's values (shortcut) ---\n"
+  print "      hit.evalue        #=> "; p hit.evalue
+  print "      hit.bit_score     #=> "; p hit.bit_score
+  print "      hit.identity      #=> "; p hit.identity
+  #print "      hit.overlap       #=> "; p hit.overlap
+
+  print "      hit.query_seq     #=> "; p hit.query_seq
+  print "      hit.midline       #=> "; p hit.midline
+  print "      hit.target_seq    #=> "; p hit.target_seq
+
+  print "      hit.query_start   #=> "; p hit.query_start
+  print "      hit.query_end     #=> "; p hit.query_end
+  print "      hit.target_start  #=> "; p hit.target_start
+  print "      hit.target_end    #=> "; p hit.target_end
+  print "      hit.lap_at        #=> "; p hit.lap_at
+  print "            --- first HSP's vaules (shortcut) ---\n"
+  print "        --- compatible/shortcut ---\n"
+
+  puts
+  print "      hit.hsps.size     #=> "; p hit.hsps.size
+  if hit.hsps.size == 0 then
+  puts  "          (HSP not found: please see blastall's -b and -v options)"
+  puts
+  else
+
+  puts
+  print "      hit.hsps.each do |hsp|\n"
+  puts
+
+  hit.hsps.each_with_index do |hsp, j|
+
+  print "# --- Bio::Blast::Default::Report::Hsp"
+  print " ([#{j}])\n"
+  puts
+  #print "        hsp.num         #=> "; p hsp.num
+  print "        hsp.bit_score   #=> "; p hsp.bit_score 
+  print "        hsp.score       #=> "; p hsp.score
+  print "        hsp.evalue      #=> "; p hsp.evalue
+  print "        hsp.identity    #=> "; p hsp.identity
+  print "        hsp.gaps        #=> "; p hsp.gaps
+  print "        hsp.positive    #=> "; p hsp.positive
+  print "        hsp.align_len   #=> "; p hsp.align_len
+  #print "        hsp.density     #=> "; p hsp.density
+
+  print "        hsp.query_frame #=> "; p hsp.query_frame
+  print "        hsp.query_from  #=> "; p hsp.query_from
+  print "        hsp.query_to    #=> "; p hsp.query_to
+
+  print "        hsp.hit_frame   #=> "; p hsp.hit_frame
+  print "        hsp.hit_from    #=> "; p hsp.hit_from
+  print "        hsp.hit_to      #=> "; p hsp.hit_to
+
+  #print "        hsp.pattern_from#=> "; p hsp.pattern_from
+  #print "        hsp.pattern_to  #=> "; p hsp.pattern_to
+
+  print "        hsp.qseq        #=> "; p hsp.qseq
+  print "        hsp.midline     #=> "; p hsp.midline
+  print "        hsp.hseq        #=> "; p hsp.hseq
+  puts
+  print "        hsp.percent_identity  #=> "; p hsp.percent_identity
+  #print "        hsp.mismatch_count    #=> "; p hsp.mismatch_count
+  #
+  print "        hsp.query_strand      #=> "; p hsp.query_strand
+  print "        hsp.hit_strand        #=> "; p hsp.hit_strand
+  print "        hsp.percent_positive  #=> "; p hsp.percent_positive
+  print "        hsp.percent_gaps      #=> "; p hsp.percent_gaps
+  puts
+
+  end #each
+  end #if hit.hsps.size == 0
+  end
+  end
+  end #ff.each
+  end #FlatFile.open
+
+end #if __FILE__ == $0
+
+######################################################################