wwood-bioruby 1.2.11

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Files changed (386) hide show
  1. data/README.rdoc +205 -0
  2. data/README_DEV.rdoc +285 -0
  3. data/VERSION.yml +4 -0
  4. data/bin/bioruby +44 -0
  5. data/bin/br_biofetch.rb +47 -0
  6. data/bin/br_bioflat.rb +293 -0
  7. data/bin/br_biogetseq.rb +45 -0
  8. data/bin/br_pmfetch.rb +421 -0
  9. data/lib/bio.rb +306 -0
  10. data/lib/bio/alignment.rb +2518 -0
  11. data/lib/bio/appl/bl2seq/report.rb +334 -0
  12. data/lib/bio/appl/blast.rb +505 -0
  13. data/lib/bio/appl/blast/ddbj.rb +142 -0
  14. data/lib/bio/appl/blast/format0.rb +1438 -0
  15. data/lib/bio/appl/blast/format8.rb +83 -0
  16. data/lib/bio/appl/blast/genomenet.rb +263 -0
  17. data/lib/bio/appl/blast/ncbioptions.rb +220 -0
  18. data/lib/bio/appl/blast/remote.rb +105 -0
  19. data/lib/bio/appl/blast/report.rb +767 -0
  20. data/lib/bio/appl/blast/rexml.rb +144 -0
  21. data/lib/bio/appl/blast/rpsblast.rb +277 -0
  22. data/lib/bio/appl/blast/wublast.rb +635 -0
  23. data/lib/bio/appl/blast/xmlparser.rb +236 -0
  24. data/lib/bio/appl/blat/report.rb +530 -0
  25. data/lib/bio/appl/clustalw.rb +219 -0
  26. data/lib/bio/appl/clustalw/report.rb +152 -0
  27. data/lib/bio/appl/emboss.rb +203 -0
  28. data/lib/bio/appl/fasta.rb +235 -0
  29. data/lib/bio/appl/fasta/format10.rb +325 -0
  30. data/lib/bio/appl/gcg/msf.rb +212 -0
  31. data/lib/bio/appl/gcg/seq.rb +195 -0
  32. data/lib/bio/appl/genscan/report.rb +552 -0
  33. data/lib/bio/appl/hmmer.rb +126 -0
  34. data/lib/bio/appl/hmmer/report.rb +683 -0
  35. data/lib/bio/appl/iprscan/report.rb +374 -0
  36. data/lib/bio/appl/mafft.rb +259 -0
  37. data/lib/bio/appl/mafft/report.rb +226 -0
  38. data/lib/bio/appl/muscle.rb +52 -0
  39. data/lib/bio/appl/paml/baseml.rb +95 -0
  40. data/lib/bio/appl/paml/baseml/report.rb +32 -0
  41. data/lib/bio/appl/paml/codeml.rb +242 -0
  42. data/lib/bio/appl/paml/codeml/rates.rb +67 -0
  43. data/lib/bio/appl/paml/codeml/report.rb +67 -0
  44. data/lib/bio/appl/paml/common.rb +348 -0
  45. data/lib/bio/appl/paml/common_report.rb +38 -0
  46. data/lib/bio/appl/paml/yn00.rb +103 -0
  47. data/lib/bio/appl/paml/yn00/report.rb +32 -0
  48. data/lib/bio/appl/phylip/alignment.rb +133 -0
  49. data/lib/bio/appl/phylip/distance_matrix.rb +96 -0
  50. data/lib/bio/appl/probcons.rb +41 -0
  51. data/lib/bio/appl/psort.rb +548 -0
  52. data/lib/bio/appl/psort/report.rb +542 -0
  53. data/lib/bio/appl/pts1.rb +263 -0
  54. data/lib/bio/appl/sim4.rb +124 -0
  55. data/lib/bio/appl/sim4/report.rb +485 -0
  56. data/lib/bio/appl/sosui/report.rb +151 -0
  57. data/lib/bio/appl/spidey/report.rb +593 -0
  58. data/lib/bio/appl/targetp/report.rb +267 -0
  59. data/lib/bio/appl/tcoffee.rb +55 -0
  60. data/lib/bio/appl/tmhmm/report.rb +231 -0
  61. data/lib/bio/command.rb +593 -0
  62. data/lib/bio/compat/features.rb +157 -0
  63. data/lib/bio/compat/references.rb +128 -0
  64. data/lib/bio/data/aa.rb +353 -0
  65. data/lib/bio/data/codontable.rb +722 -0
  66. data/lib/bio/data/na.rb +223 -0
  67. data/lib/bio/db.rb +329 -0
  68. data/lib/bio/db/aaindex.rb +357 -0
  69. data/lib/bio/db/biosql/biosql_to_biosequence.rb +67 -0
  70. data/lib/bio/db/biosql/sequence.rb +508 -0
  71. data/lib/bio/db/embl/common.rb +352 -0
  72. data/lib/bio/db/embl/embl.rb +500 -0
  73. data/lib/bio/db/embl/embl_to_biosequence.rb +85 -0
  74. data/lib/bio/db/embl/format_embl.rb +190 -0
  75. data/lib/bio/db/embl/sptr.rb +1283 -0
  76. data/lib/bio/db/embl/swissprot.rb +42 -0
  77. data/lib/bio/db/embl/trembl.rb +41 -0
  78. data/lib/bio/db/embl/uniprot.rb +42 -0
  79. data/lib/bio/db/fantom.rb +597 -0
  80. data/lib/bio/db/fasta.rb +410 -0
  81. data/lib/bio/db/fasta/defline.rb +532 -0
  82. data/lib/bio/db/fasta/fasta_to_biosequence.rb +63 -0
  83. data/lib/bio/db/fasta/format_fasta.rb +97 -0
  84. data/lib/bio/db/genbank/common.rb +307 -0
  85. data/lib/bio/db/genbank/ddbj.rb +22 -0
  86. data/lib/bio/db/genbank/format_genbank.rb +187 -0
  87. data/lib/bio/db/genbank/genbank.rb +250 -0
  88. data/lib/bio/db/genbank/genbank_to_biosequence.rb +86 -0
  89. data/lib/bio/db/genbank/genpept.rb +60 -0
  90. data/lib/bio/db/genbank/refseq.rb +18 -0
  91. data/lib/bio/db/gff.rb +1846 -0
  92. data/lib/bio/db/go.rb +481 -0
  93. data/lib/bio/db/kegg/brite.rb +41 -0
  94. data/lib/bio/db/kegg/compound.rb +131 -0
  95. data/lib/bio/db/kegg/drug.rb +98 -0
  96. data/lib/bio/db/kegg/enzyme.rb +148 -0
  97. data/lib/bio/db/kegg/expression.rb +155 -0
  98. data/lib/bio/db/kegg/genes.rb +263 -0
  99. data/lib/bio/db/kegg/genome.rb +241 -0
  100. data/lib/bio/db/kegg/glycan.rb +166 -0
  101. data/lib/bio/db/kegg/keggtab.rb +357 -0
  102. data/lib/bio/db/kegg/kgml.rb +256 -0
  103. data/lib/bio/db/kegg/orthology.rb +136 -0
  104. data/lib/bio/db/kegg/reaction.rb +82 -0
  105. data/lib/bio/db/kegg/taxonomy.rb +331 -0
  106. data/lib/bio/db/lasergene.rb +209 -0
  107. data/lib/bio/db/litdb.rb +107 -0
  108. data/lib/bio/db/medline.rb +326 -0
  109. data/lib/bio/db/nbrf.rb +191 -0
  110. data/lib/bio/db/newick.rb +658 -0
  111. data/lib/bio/db/nexus.rb +1854 -0
  112. data/lib/bio/db/pdb.rb +29 -0
  113. data/lib/bio/db/pdb/atom.rb +77 -0
  114. data/lib/bio/db/pdb/chain.rb +210 -0
  115. data/lib/bio/db/pdb/chemicalcomponent.rb +224 -0
  116. data/lib/bio/db/pdb/model.rb +148 -0
  117. data/lib/bio/db/pdb/pdb.rb +1911 -0
  118. data/lib/bio/db/pdb/residue.rb +176 -0
  119. data/lib/bio/db/pdb/utils.rb +399 -0
  120. data/lib/bio/db/prosite.rb +597 -0
  121. data/lib/bio/db/rebase.rb +456 -0
  122. data/lib/bio/db/soft.rb +404 -0
  123. data/lib/bio/db/transfac.rb +375 -0
  124. data/lib/bio/db/url.rb +42 -0
  125. data/lib/bio/feature.rb +139 -0
  126. data/lib/bio/io/biosql/biodatabase.rb +64 -0
  127. data/lib/bio/io/biosql/bioentry.rb +29 -0
  128. data/lib/bio/io/biosql/bioentry_dbxref.rb +11 -0
  129. data/lib/bio/io/biosql/bioentry_path.rb +12 -0
  130. data/lib/bio/io/biosql/bioentry_qualifier_value.rb +10 -0
  131. data/lib/bio/io/biosql/bioentry_reference.rb +10 -0
  132. data/lib/bio/io/biosql/bioentry_relationship.rb +10 -0
  133. data/lib/bio/io/biosql/biosequence.rb +11 -0
  134. data/lib/bio/io/biosql/comment.rb +7 -0
  135. data/lib/bio/io/biosql/config/database.yml +20 -0
  136. data/lib/bio/io/biosql/dbxref.rb +13 -0
  137. data/lib/bio/io/biosql/dbxref_qualifier_value.rb +12 -0
  138. data/lib/bio/io/biosql/location.rb +32 -0
  139. data/lib/bio/io/biosql/location_qualifier_value.rb +11 -0
  140. data/lib/bio/io/biosql/ontology.rb +10 -0
  141. data/lib/bio/io/biosql/reference.rb +9 -0
  142. data/lib/bio/io/biosql/seqfeature.rb +32 -0
  143. data/lib/bio/io/biosql/seqfeature_dbxref.rb +11 -0
  144. data/lib/bio/io/biosql/seqfeature_path.rb +11 -0
  145. data/lib/bio/io/biosql/seqfeature_qualifier_value.rb +20 -0
  146. data/lib/bio/io/biosql/seqfeature_relationship.rb +11 -0
  147. data/lib/bio/io/biosql/taxon.rb +12 -0
  148. data/lib/bio/io/biosql/taxon_name.rb +9 -0
  149. data/lib/bio/io/biosql/term.rb +27 -0
  150. data/lib/bio/io/biosql/term_dbxref.rb +11 -0
  151. data/lib/bio/io/biosql/term_path.rb +12 -0
  152. data/lib/bio/io/biosql/term_relationship.rb +13 -0
  153. data/lib/bio/io/biosql/term_relationship_term.rb +11 -0
  154. data/lib/bio/io/biosql/term_synonym.rb +10 -0
  155. data/lib/bio/io/das.rb +461 -0
  156. data/lib/bio/io/dbget.rb +194 -0
  157. data/lib/bio/io/ddbjxml.rb +638 -0
  158. data/lib/bio/io/ebisoap.rb +158 -0
  159. data/lib/bio/io/ensembl.rb +229 -0
  160. data/lib/bio/io/fastacmd.rb +163 -0
  161. data/lib/bio/io/fetch.rb +195 -0
  162. data/lib/bio/io/flatfile.rb +482 -0
  163. data/lib/bio/io/flatfile/autodetection.rb +545 -0
  164. data/lib/bio/io/flatfile/bdb.rb +253 -0
  165. data/lib/bio/io/flatfile/buffer.rb +237 -0
  166. data/lib/bio/io/flatfile/index.rb +1381 -0
  167. data/lib/bio/io/flatfile/indexer.rb +805 -0
  168. data/lib/bio/io/flatfile/splitter.rb +297 -0
  169. data/lib/bio/io/higet.rb +73 -0
  170. data/lib/bio/io/hinv.rb +442 -0
  171. data/lib/bio/io/keggapi.rb +805 -0
  172. data/lib/bio/io/ncbirest.rb +733 -0
  173. data/lib/bio/io/ncbisoap.rb +155 -0
  174. data/lib/bio/io/pubmed.rb +307 -0
  175. data/lib/bio/io/registry.rb +292 -0
  176. data/lib/bio/io/soapwsdl.rb +119 -0
  177. data/lib/bio/io/sql.rb +186 -0
  178. data/lib/bio/location.rb +867 -0
  179. data/lib/bio/map.rb +410 -0
  180. data/lib/bio/pathway.rb +960 -0
  181. data/lib/bio/reference.rb +602 -0
  182. data/lib/bio/sequence.rb +456 -0
  183. data/lib/bio/sequence/aa.rb +152 -0
  184. data/lib/bio/sequence/adapter.rb +108 -0
  185. data/lib/bio/sequence/common.rb +310 -0
  186. data/lib/bio/sequence/compat.rb +123 -0
  187. data/lib/bio/sequence/dblink.rb +54 -0
  188. data/lib/bio/sequence/format.rb +358 -0
  189. data/lib/bio/sequence/format_raw.rb +23 -0
  190. data/lib/bio/sequence/generic.rb +24 -0
  191. data/lib/bio/sequence/na.rb +491 -0
  192. data/lib/bio/shell.rb +44 -0
  193. data/lib/bio/shell/core.rb +578 -0
  194. data/lib/bio/shell/demo.rb +146 -0
  195. data/lib/bio/shell/interface.rb +218 -0
  196. data/lib/bio/shell/irb.rb +95 -0
  197. data/lib/bio/shell/object.rb +71 -0
  198. data/lib/bio/shell/plugin/blast.rb +42 -0
  199. data/lib/bio/shell/plugin/codon.rb +218 -0
  200. data/lib/bio/shell/plugin/das.rb +58 -0
  201. data/lib/bio/shell/plugin/emboss.rb +23 -0
  202. data/lib/bio/shell/plugin/entry.rb +105 -0
  203. data/lib/bio/shell/plugin/flatfile.rb +101 -0
  204. data/lib/bio/shell/plugin/keggapi.rb +181 -0
  205. data/lib/bio/shell/plugin/midi.rb +430 -0
  206. data/lib/bio/shell/plugin/obda.rb +45 -0
  207. data/lib/bio/shell/plugin/psort.rb +56 -0
  208. data/lib/bio/shell/plugin/seq.rb +247 -0
  209. data/lib/bio/shell/plugin/soap.rb +87 -0
  210. data/lib/bio/shell/rails/vendor/plugins/bioruby/generators/bioruby/bioruby_generator.rb +29 -0
  211. data/lib/bio/shell/rails/vendor/plugins/bioruby/generators/bioruby/templates/_classes.rhtml +4 -0
  212. data/lib/bio/shell/rails/vendor/plugins/bioruby/generators/bioruby/templates/_log.rhtml +27 -0
  213. data/lib/bio/shell/rails/vendor/plugins/bioruby/generators/bioruby/templates/_methods.rhtml +11 -0
  214. data/lib/bio/shell/rails/vendor/plugins/bioruby/generators/bioruby/templates/_modules.rhtml +4 -0
  215. data/lib/bio/shell/rails/vendor/plugins/bioruby/generators/bioruby/templates/_variables.rhtml +7 -0
  216. data/lib/bio/shell/rails/vendor/plugins/bioruby/generators/bioruby/templates/bioruby-bg.gif +0 -0
  217. data/lib/bio/shell/rails/vendor/plugins/bioruby/generators/bioruby/templates/bioruby-gem.png +0 -0
  218. data/lib/bio/shell/rails/vendor/plugins/bioruby/generators/bioruby/templates/bioruby-link.gif +0 -0
  219. data/lib/bio/shell/rails/vendor/plugins/bioruby/generators/bioruby/templates/bioruby.css +368 -0
  220. data/lib/bio/shell/rails/vendor/plugins/bioruby/generators/bioruby/templates/bioruby.rhtml +47 -0
  221. data/lib/bio/shell/rails/vendor/plugins/bioruby/generators/bioruby/templates/bioruby_controller.rb +144 -0
  222. data/lib/bio/shell/rails/vendor/plugins/bioruby/generators/bioruby/templates/bioruby_helper.rb +47 -0
  223. data/lib/bio/shell/rails/vendor/plugins/bioruby/generators/bioruby/templates/commands.rhtml +8 -0
  224. data/lib/bio/shell/rails/vendor/plugins/bioruby/generators/bioruby/templates/history.rhtml +10 -0
  225. data/lib/bio/shell/rails/vendor/plugins/bioruby/generators/bioruby/templates/index.rhtml +26 -0
  226. data/lib/bio/shell/rails/vendor/plugins/bioruby/generators/bioruby/templates/spinner.gif +0 -0
  227. data/lib/bio/shell/script.rb +25 -0
  228. data/lib/bio/shell/setup.rb +109 -0
  229. data/lib/bio/shell/web.rb +102 -0
  230. data/lib/bio/tree.rb +852 -0
  231. data/lib/bio/util/color_scheme.rb +191 -0
  232. data/lib/bio/util/color_scheme/buried.rb +59 -0
  233. data/lib/bio/util/color_scheme/helix.rb +59 -0
  234. data/lib/bio/util/color_scheme/hydropathy.rb +64 -0
  235. data/lib/bio/util/color_scheme/nucleotide.rb +31 -0
  236. data/lib/bio/util/color_scheme/strand.rb +59 -0
  237. data/lib/bio/util/color_scheme/taylor.rb +50 -0
  238. data/lib/bio/util/color_scheme/turn.rb +59 -0
  239. data/lib/bio/util/color_scheme/zappo.rb +50 -0
  240. data/lib/bio/util/contingency_table.rb +370 -0
  241. data/lib/bio/util/restriction_enzyme.rb +228 -0
  242. data/lib/bio/util/restriction_enzyme/analysis.rb +249 -0
  243. data/lib/bio/util/restriction_enzyme/analysis_basic.rb +217 -0
  244. data/lib/bio/util/restriction_enzyme/cut_symbol.rb +107 -0
  245. data/lib/bio/util/restriction_enzyme/double_stranded.rb +321 -0
  246. data/lib/bio/util/restriction_enzyme/double_stranded/aligned_strands.rb +130 -0
  247. data/lib/bio/util/restriction_enzyme/double_stranded/cut_location_pair.rb +103 -0
  248. data/lib/bio/util/restriction_enzyme/double_stranded/cut_location_pair_in_enzyme_notation.rb +38 -0
  249. data/lib/bio/util/restriction_enzyme/double_stranded/cut_locations.rb +76 -0
  250. data/lib/bio/util/restriction_enzyme/double_stranded/cut_locations_in_enzyme_notation.rb +107 -0
  251. data/lib/bio/util/restriction_enzyme/enzymes.yaml +7061 -0
  252. data/lib/bio/util/restriction_enzyme/range/cut_range.rb +24 -0
  253. data/lib/bio/util/restriction_enzyme/range/cut_ranges.rb +47 -0
  254. data/lib/bio/util/restriction_enzyme/range/horizontal_cut_range.rb +67 -0
  255. data/lib/bio/util/restriction_enzyme/range/sequence_range.rb +257 -0
  256. data/lib/bio/util/restriction_enzyme/range/sequence_range/calculated_cuts.rb +242 -0
  257. data/lib/bio/util/restriction_enzyme/range/sequence_range/fragment.rb +51 -0
  258. data/lib/bio/util/restriction_enzyme/range/sequence_range/fragments.rb +41 -0
  259. data/lib/bio/util/restriction_enzyme/range/vertical_cut_range.rb +77 -0
  260. data/lib/bio/util/restriction_enzyme/single_strand.rb +200 -0
  261. data/lib/bio/util/restriction_enzyme/single_strand/cut_locations_in_enzyme_notation.rb +135 -0
  262. data/lib/bio/util/restriction_enzyme/single_strand_complement.rb +23 -0
  263. data/lib/bio/util/restriction_enzyme/string_formatting.rb +111 -0
  264. data/lib/bio/util/sirna.rb +288 -0
  265. data/test/data/HMMER/hmmpfam.out +64 -0
  266. data/test/data/HMMER/hmmsearch.out +88 -0
  267. data/test/data/SOSUI/sample.report +11 -0
  268. data/test/data/TMHMM/sample.report +21 -0
  269. data/test/data/aaindex/DAYM780301 +30 -0
  270. data/test/data/aaindex/PRAM900102 +20 -0
  271. data/test/data/bl2seq/cd8a_cd8b_blastp.bl2seq +53 -0
  272. data/test/data/bl2seq/cd8a_p53_e-5blastp.bl2seq +37 -0
  273. data/test/data/blast/2.2.15.blastp.m7 +876 -0
  274. data/test/data/blast/b0002.faa +15 -0
  275. data/test/data/blast/b0002.faa.m0 +128 -0
  276. data/test/data/blast/b0002.faa.m7 +65 -0
  277. data/test/data/blast/b0002.faa.m8 +1 -0
  278. data/test/data/blast/blastp-multi.m7 +188 -0
  279. data/test/data/command/echoarg2.bat +1 -0
  280. data/test/data/embl/AB090716.embl +65 -0
  281. data/test/data/embl/AB090716.embl.rel89 +63 -0
  282. data/test/data/fasta/example1.txt +75 -0
  283. data/test/data/fasta/example2.txt +21 -0
  284. data/test/data/genscan/sample.report +63 -0
  285. data/test/data/iprscan/merged.raw +32 -0
  286. data/test/data/iprscan/merged.txt +74 -0
  287. data/test/data/paml/codeml/control_file.txt +30 -0
  288. data/test/data/paml/codeml/output.txt +78 -0
  289. data/test/data/paml/codeml/rates +217 -0
  290. data/test/data/prosite/prosite.dat +2233 -0
  291. data/test/data/refseq/nm_126355.entret +64 -0
  292. data/test/data/rpsblast/misc.rpsblast +193 -0
  293. data/test/data/soft/GDS100_partial.soft +92 -0
  294. data/test/data/soft/GSE3457_family_partial.soft +874 -0
  295. data/test/data/uniprot/p53_human.uniprot +1456 -0
  296. data/test/functional/bio/appl/test_pts1.rb +115 -0
  297. data/test/functional/bio/io/test_ensembl.rb +229 -0
  298. data/test/functional/bio/io/test_soapwsdl.rb +52 -0
  299. data/test/functional/bio/sequence/test_output_embl.rb +51 -0
  300. data/test/functional/bio/test_command.rb +301 -0
  301. data/test/runner.rb +14 -0
  302. data/test/unit/bio/appl/bl2seq/test_report.rb +134 -0
  303. data/test/unit/bio/appl/blast/test_ncbioptions.rb +112 -0
  304. data/test/unit/bio/appl/blast/test_report.rb +1135 -0
  305. data/test/unit/bio/appl/blast/test_rpsblast.rb +398 -0
  306. data/test/unit/bio/appl/genscan/test_report.rb +182 -0
  307. data/test/unit/bio/appl/hmmer/test_report.rb +342 -0
  308. data/test/unit/bio/appl/iprscan/test_report.rb +338 -0
  309. data/test/unit/bio/appl/mafft/test_report.rb +63 -0
  310. data/test/unit/bio/appl/paml/codeml/test_rates.rb +45 -0
  311. data/test/unit/bio/appl/paml/codeml/test_report.rb +45 -0
  312. data/test/unit/bio/appl/paml/test_codeml.rb +174 -0
  313. data/test/unit/bio/appl/sosui/test_report.rb +81 -0
  314. data/test/unit/bio/appl/targetp/test_report.rb +146 -0
  315. data/test/unit/bio/appl/test_blast.rb +277 -0
  316. data/test/unit/bio/appl/test_fasta.rb +130 -0
  317. data/test/unit/bio/appl/test_psort.rb +57 -0
  318. data/test/unit/bio/appl/test_pts1.rb +77 -0
  319. data/test/unit/bio/appl/tmhmm/test_report.rb +126 -0
  320. data/test/unit/bio/data/test_aa.rb +90 -0
  321. data/test/unit/bio/data/test_codontable.rb +107 -0
  322. data/test/unit/bio/data/test_na.rb +80 -0
  323. data/test/unit/bio/db/embl/test_common.rb +117 -0
  324. data/test/unit/bio/db/embl/test_embl.rb +214 -0
  325. data/test/unit/bio/db/embl/test_embl_rel89.rb +219 -0
  326. data/test/unit/bio/db/embl/test_embl_to_bioseq.rb +203 -0
  327. data/test/unit/bio/db/embl/test_sptr.rb +1812 -0
  328. data/test/unit/bio/db/embl/test_uniprot.rb +31 -0
  329. data/test/unit/bio/db/kegg/test_genes.rb +45 -0
  330. data/test/unit/bio/db/pdb/test_pdb.rb +152 -0
  331. data/test/unit/bio/db/test_aaindex.rb +197 -0
  332. data/test/unit/bio/db/test_fasta.rb +250 -0
  333. data/test/unit/bio/db/test_gff.rb +1190 -0
  334. data/test/unit/bio/db/test_lasergene.rb +95 -0
  335. data/test/unit/bio/db/test_medline.rb +127 -0
  336. data/test/unit/bio/db/test_newick.rb +293 -0
  337. data/test/unit/bio/db/test_nexus.rb +364 -0
  338. data/test/unit/bio/db/test_prosite.rb +1437 -0
  339. data/test/unit/bio/db/test_rebase.rb +101 -0
  340. data/test/unit/bio/db/test_soft.rb +138 -0
  341. data/test/unit/bio/db/test_url.rb +36 -0
  342. data/test/unit/bio/io/flatfile/test_autodetection.rb +375 -0
  343. data/test/unit/bio/io/flatfile/test_buffer.rb +251 -0
  344. data/test/unit/bio/io/flatfile/test_splitter.rb +369 -0
  345. data/test/unit/bio/io/test_ddbjxml.rb +80 -0
  346. data/test/unit/bio/io/test_ensembl.rb +109 -0
  347. data/test/unit/bio/io/test_fastacmd.rb +42 -0
  348. data/test/unit/bio/io/test_flatfile.rb +505 -0
  349. data/test/unit/bio/io/test_soapwsdl.rb +32 -0
  350. data/test/unit/bio/sequence/test_aa.rb +115 -0
  351. data/test/unit/bio/sequence/test_common.rb +373 -0
  352. data/test/unit/bio/sequence/test_compat.rb +69 -0
  353. data/test/unit/bio/sequence/test_dblink.rb +58 -0
  354. data/test/unit/bio/sequence/test_na.rb +330 -0
  355. data/test/unit/bio/shell/plugin/test_seq.rb +185 -0
  356. data/test/unit/bio/test_alignment.rb +1025 -0
  357. data/test/unit/bio/test_command.rb +349 -0
  358. data/test/unit/bio/test_db.rb +96 -0
  359. data/test/unit/bio/test_feature.rb +144 -0
  360. data/test/unit/bio/test_location.rb +599 -0
  361. data/test/unit/bio/test_map.rb +230 -0
  362. data/test/unit/bio/test_pathway.rb +499 -0
  363. data/test/unit/bio/test_reference.rb +252 -0
  364. data/test/unit/bio/test_sequence.rb +329 -0
  365. data/test/unit/bio/test_shell.rb +18 -0
  366. data/test/unit/bio/test_tree.rb +593 -0
  367. data/test/unit/bio/util/restriction_enzyme/analysis/test_calculated_cuts.rb +299 -0
  368. data/test/unit/bio/util/restriction_enzyme/analysis/test_cut_ranges.rb +103 -0
  369. data/test/unit/bio/util/restriction_enzyme/analysis/test_sequence_range.rb +240 -0
  370. data/test/unit/bio/util/restriction_enzyme/double_stranded/test_aligned_strands.rb +101 -0
  371. data/test/unit/bio/util/restriction_enzyme/double_stranded/test_cut_location_pair.rb +75 -0
  372. data/test/unit/bio/util/restriction_enzyme/double_stranded/test_cut_location_pair_in_enzyme_notation.rb +73 -0
  373. data/test/unit/bio/util/restriction_enzyme/double_stranded/test_cut_locations.rb +53 -0
  374. data/test/unit/bio/util/restriction_enzyme/double_stranded/test_cut_locations_in_enzyme_notation.rb +104 -0
  375. data/test/unit/bio/util/restriction_enzyme/single_strand/test_cut_locations_in_enzyme_notation.rb +83 -0
  376. data/test/unit/bio/util/restriction_enzyme/test_analysis.rb +246 -0
  377. data/test/unit/bio/util/restriction_enzyme/test_cut_symbol.rb +44 -0
  378. data/test/unit/bio/util/restriction_enzyme/test_double_stranded.rb +115 -0
  379. data/test/unit/bio/util/restriction_enzyme/test_single_strand.rb +147 -0
  380. data/test/unit/bio/util/restriction_enzyme/test_single_strand_complement.rb +147 -0
  381. data/test/unit/bio/util/restriction_enzyme/test_string_formatting.rb +60 -0
  382. data/test/unit/bio/util/test_color_scheme.rb +33 -0
  383. data/test/unit/bio/util/test_contingency_table.rb +94 -0
  384. data/test/unit/bio/util/test_restriction_enzyme.rb +42 -0
  385. data/test/unit/bio/util/test_sirna.rb +245 -0
  386. metadata +543 -0
@@ -0,0 +1,212 @@
1
+ #
2
+ # = bio/appl/gcg/msf.rb - GCG multiple sequence alignment (.msf) parser class
3
+ #
4
+ # Copyright:: Copyright (C) 2003, 2006
5
+ # Naohisa Goto <ng@bioruby.org>
6
+ # License:: The Ruby License
7
+ #
8
+ # $Id: msf.rb,v 1.2 2007/04/05 23:35:39 trevor Exp $
9
+ #
10
+ # = About Bio::GCG::Msf
11
+ #
12
+ # Please refer document of Bio::GCG::Msf.
13
+ #
14
+
15
+ #---
16
+ # (depends on autoload)
17
+ #require 'bio/appl/gcg/seq'
18
+ #+++
19
+
20
+ module Bio
21
+ module GCG
22
+
23
+ # The msf is a multiple sequence alignment format developed by Wisconsin.
24
+ # Bio::GCG::Msf is a msf format parser.
25
+ class Msf #< DB
26
+
27
+ # delimiter used by Bio::FlatFile
28
+ DELIMITER = RS = nil
29
+
30
+ # Creates a new Msf object.
31
+ def initialize(str)
32
+ str = str.sub(/\A[\r\n]+/, '')
33
+ if /^\!\![A-Z]+\_MULTIPLE\_ALIGNMNENT/ =~ str[/.*/] then
34
+ @heading = str[/.*/] # '!!NA_MULTIPLE_ALIGNMENT 1.0' or like this
35
+ str.sub!(/.*/, '')
36
+ end
37
+ str.sub!(/.*\.\.$/m, '')
38
+ @description = $&.to_s.sub(/^.*\.\.$/, '').to_s
39
+ d = $&.to_s
40
+ if m = /(.+)\s+MSF\:\s+(\d+)\s+Type\:\s+(\w)\s+(.+)\s+(Comp)?Check\:\s+(\d+)/.match(d) then
41
+ @entry_id = m[1].to_s.strip
42
+ @length = (m[2] ? m[2].to_i : nil)
43
+ @seq_type = m[3]
44
+ @date = m[4].to_s.strip
45
+ @checksum = (m[6] ? m[6].to_i : nil)
46
+ end
47
+
48
+ str.sub!(/.*\/\/$/m, '')
49
+ a = $&.to_s.split(/^/)
50
+ @seq_info = []
51
+ a.each do |x|
52
+ if /Name\: / =~ x then
53
+ s = {}
54
+ x.scan(/(\S+)\: +(\S*)/) { |y| s[$1] = $2 }
55
+ @seq_info << s
56
+ end
57
+ end
58
+
59
+ @data = str
60
+ @description.sub!(/\A(\r\n|\r|\n)/, '')
61
+ @align = nil
62
+ end
63
+
64
+ # description
65
+ attr_reader :description
66
+
67
+ # ID of the alignment
68
+ attr_reader :entry_id
69
+
70
+ # alignment length
71
+ attr_reader :length
72
+
73
+ # sequence type ("N" for DNA/RNA or "P" for protein)
74
+ attr_reader :seq_type
75
+
76
+ # date
77
+ attr_reader :date
78
+
79
+ # checksum
80
+ attr_reader :checksum
81
+
82
+ # heading
83
+ # ('!!NA_MULTIPLE_ALIGNMENT 1.0' or whatever like this)
84
+ attr_reader :heading
85
+
86
+ #---
87
+ ## data (internally used, will be obsoleted)
88
+ #attr_reader :data
89
+ #
90
+ ## seq. info. (internally used, will be obsoleted)
91
+ #attr_reader :seq_info
92
+ #+++
93
+
94
+ # symbol comparison table
95
+ def symbol_comparison_table
96
+ unless defined?(@symbol_comparison_table)
97
+ /Symbol comparison table\: +(\S+)/ =~ @description
98
+ @symbol_comparison_table = $1
99
+ end
100
+ @symbol_comparison_table
101
+ end
102
+
103
+ # gap weight
104
+ def gap_weight
105
+ unless defined?(@gap_weight)
106
+ /GapWeight\: +(\S+)/ =~ @description
107
+ @gap_weight = $1
108
+ end
109
+ @gap_weight
110
+ end
111
+
112
+ # gap length weight
113
+ def gap_length_weight
114
+ unless defined?(@gap_length_weight)
115
+ /GapLengthWeight\: +(\S+)/ =~ @description
116
+ @gap_length_weight = $1
117
+ end
118
+ @gap_length_weight
119
+ end
120
+
121
+ # CompCheck field
122
+ def compcheck
123
+ unless defined?(@compcheck)
124
+ if /CompCheck\: +(\d+)/ =~ @description then
125
+ @compcheck = $1.to_i
126
+ else
127
+ @compcheck = nil
128
+ end
129
+ end
130
+ @compcheck
131
+ end
132
+
133
+ # parsing
134
+ def do_parse
135
+ return if @align
136
+ a = @data.strip.split(/\n\n/)
137
+ @seq_data = Array.new(@seq_info.size)
138
+ @seq_data.collect! { |x| Array.new }
139
+ a.each do |x|
140
+ b = x.split(/\n/)
141
+ nw = 0
142
+ if b.size > @seq_info.size then
143
+ if /^ +/ =~ b.shift.to_s
144
+ nw = $&.to_s.length
145
+ end
146
+ end
147
+ if nw > 0 then
148
+ b.each_with_index { |y, i| y[0, nw] = ''; @seq_data[i] << y }
149
+ else
150
+ b.each_with_index { |y, i|
151
+ @seq_data[i] << y.strip.split(/ +/, 2)[1].to_s
152
+ }
153
+ end
154
+ end
155
+
156
+ case seq_type
157
+ when 'P', 'p'
158
+ k = Bio::Sequence::AA
159
+ when 'N', 'n'
160
+ k = Bio::Sequence::NA
161
+ else
162
+ k = Bio::Sequence::Generic
163
+ end
164
+ @seq_data.collect! do |x|
165
+ y = x.join('')
166
+ y.gsub!(/[\s\d]+/, '')
167
+ k.new(y)
168
+ end
169
+
170
+ aln = Bio::Alignment.new
171
+ @seq_data.each_with_index do |x, i|
172
+ aln.store(@seq_info[i]['Name'], x)
173
+ end
174
+ @align = aln
175
+ end
176
+ private :do_parse
177
+
178
+ # returns Bio::Alignment object.
179
+ def alignment
180
+ do_parse
181
+ @align
182
+ end
183
+
184
+ # gets seq data (used internally) (will be obsoleted)
185
+ def seq_data
186
+ do_parse
187
+ @seq_data
188
+ end
189
+
190
+ # validates checksum
191
+ def validate_checksum
192
+ do_parse
193
+ valid = true
194
+ total = 0
195
+ @seq_data.each_with_index do |x, i|
196
+ sum = Bio::GCG::Seq.calc_checksum(x)
197
+ if sum != @seq_info[i]['Check'].to_i
198
+ valid = false
199
+ break
200
+ end
201
+ total += sum
202
+ end
203
+ return false unless valid
204
+ if @checksum != 0 # "Check:" field of BioPerl is always 0
205
+ valid = ((total % 10000) == @checksum)
206
+ end
207
+ valid
208
+ end
209
+
210
+ end #class Msf
211
+ end #module GCG
212
+ end # module Bio
@@ -0,0 +1,195 @@
1
+ #
2
+ # = bio/appl/gcg/seq.rb - GCG sequence file format class (.seq/.pep file)
3
+ #
4
+ # Copyright:: Copyright (C) 2003, 2006
5
+ # Naohisa Goto <ng@bioruby.org>
6
+ # License:: The Ruby License
7
+ #
8
+ # $Id: seq.rb,v 1.3 2007/04/05 23:35:39 trevor Exp $
9
+ #
10
+ # = About Bio::GCG::Seq
11
+ #
12
+ # Please refer document of Bio::GCG::Seq.
13
+ #
14
+
15
+ module Bio
16
+ module GCG
17
+
18
+ #
19
+ # = Bio::GCG::Seq
20
+ #
21
+ # This is GCG sequence file format (.seq or .pep) parser class.
22
+ #
23
+ # = References
24
+ #
25
+ # * Information about GCG Wisconsin Package(R)
26
+ # http://www.accelrys.com/products/gcg_wisconsin_package .
27
+ # * EMBOSS sequence formats
28
+ # http://www.hgmp.mrc.ac.uk/Software/EMBOSS/Themes/SequenceFormats.html
29
+ # * BioPerl document
30
+ # http://docs.bioperl.org/releases/bioperl-1.2.3/Bio/SeqIO/gcg.html
31
+ class Seq #< DB
32
+
33
+ # delimiter used by Bio::FlatFile
34
+ DELIMITER = RS = nil
35
+
36
+ # Creates new instance of this class.
37
+ # str must be a GCG seq formatted string.
38
+ def initialize(str)
39
+ @heading = str[/.*/] # '!!NA_SEQUENCE 1.0' or like this
40
+ str = str.sub(/.*/, '')
41
+ str.sub!(/.*\.\.$/m, '')
42
+ @definition = $&.to_s.sub(/^.*\.\.$/, '').to_s
43
+ desc = $&.to_s
44
+ if m = /(.+)\s+Length\:\s+(\d+)\s+(.+)\s+Type\:\s+(\w)\s+Check\:\s+(\d+)/.match(desc) then
45
+ @entry_id = m[1].to_s.strip
46
+ @length = (m[2] ? m[2].to_i : nil)
47
+ @date = m[3].to_s.strip
48
+ @seq_type = m[4]
49
+ @checksum = (m[5] ? m[5].to_i : nil)
50
+ end
51
+ @data = str
52
+ @seq = nil
53
+ @definition.strip!
54
+ end
55
+
56
+ # ID field.
57
+ attr_reader :entry_id
58
+
59
+ # Description field.
60
+ attr_reader :definition
61
+
62
+ # "Length:" field.
63
+ # Note that sometimes this might differ from real sequence length.
64
+ attr_reader :length
65
+
66
+ # Date field of this entry.
67
+ attr_reader :date
68
+
69
+ # "Type:" field, which indicates sequence type.
70
+ # "N" means nucleic acid sequence, "P" means protein sequence.
71
+ attr_reader :seq_type
72
+
73
+ # "Check:" field, which indicates checksum of current sequence.
74
+ attr_reader :checksum
75
+
76
+ # heading
77
+ # ('!!NA_SEQUENCE 1.0' or whatever like this)
78
+ attr_reader :heading
79
+
80
+ #---
81
+ ## data (internally used, will be obsoleted)
82
+ #attr_reader :data
83
+ #+++
84
+
85
+ # Sequence data.
86
+ # The class of the sequence is Bio::Sequence::NA, Bio::Sequence::AA
87
+ # or Bio::Sequence::Generic, according to the sequence type.
88
+ def seq
89
+ unless @seq then
90
+ case @seq_type
91
+ when 'N', 'n'
92
+ k = Bio::Sequence::NA
93
+ when 'P', 'p'
94
+ k = Bio::Sequence::AA
95
+ else
96
+ k = Bio::Sequence
97
+ end
98
+ @seq = k.new(@data.tr('^-a-zA-Z.~', ''))
99
+ end
100
+ @seq
101
+ end
102
+
103
+ # If you know the sequence is AA, use this method.
104
+ # Returns a Bio::Sequence::AA object.
105
+ #
106
+ # If you call naseq for protein sequence,
107
+ # or aaseq for nucleic sequence, RuntimeError will be raised.
108
+ def aaseq
109
+ if seq.is_a?(Bio::Sequence::AA) then
110
+ @seq
111
+ else
112
+ raise 'seq_type != \'P\''
113
+ end
114
+ end
115
+
116
+ # If you know the sequence is NA, use this method.
117
+ # Returens a Bio::Sequence::NA object.
118
+ #
119
+ # If you call naseq for protein sequence,
120
+ # or aaseq for nucleic sequence, RuntimeError will be raised.
121
+ def naseq
122
+ if seq.is_a?(Bio::Sequence::NA) then
123
+ @seq
124
+ else
125
+ raise 'seq_type != \'N\''
126
+ end
127
+ end
128
+
129
+ # Validates checksum.
130
+ # If validation succeeds, returns true.
131
+ # Otherwise, returns false.
132
+ def validate_checksum
133
+ checksum == self.class.calc_checksum(seq)
134
+ end
135
+
136
+ #---
137
+ # class methods
138
+ #+++
139
+
140
+ # Calculates checksum from given string.
141
+ def self.calc_checksum(str)
142
+ # Reference: Bio::SeqIO::gcg of BioPerl-1.2.3
143
+ idx = 0
144
+ sum = 0
145
+ str.upcase.tr('^A-Z.~', '').each_byte do |c|
146
+ idx += 1
147
+ sum += idx * c
148
+ idx = 0 if idx >= 57
149
+ end
150
+ (sum % 10000)
151
+ end
152
+
153
+ # Creates a new GCG sequence format text.
154
+ # Parameters can be omitted.
155
+ #
156
+ # Examples:
157
+ # Bio::GCG::Seq.to_gcg(:definition=>'H.sapiens DNA',
158
+ # :seq_type=>'N', :entry_id=>'gi-1234567',
159
+ # :seq=>seq, :date=>date)
160
+ #
161
+ def self.to_gcg(hash)
162
+ seq = hash[:seq]
163
+ if seq.is_a?(Bio::Sequence::NA) then
164
+ seq_type = 'N'
165
+ elsif seq.is_a?(Bio::Sequence::AA) then
166
+ seq_type = 'P'
167
+ else
168
+ seq_type = (hash[:seq_type] or 'P')
169
+ end
170
+ if seq_type == 'N' then
171
+ head = '!!NA_SEQUENCE 1.0'
172
+ else
173
+ head = '!!AA_SEQUENCE 1.0'
174
+ end
175
+ date = (hash[:date] or Time.now.strftime('%B %d, %Y %H:%M'))
176
+ entry_id = hash[:entry_id].to_s.strip
177
+ len = seq.length
178
+ checksum = self.calc_checksum(seq)
179
+ definition = hash[:definition].to_s.strip
180
+ seq = seq.upcase.gsub(/.{1,50}/, "\\0\n")
181
+ seq.gsub!(/.{10}/, "\\0 ")
182
+ w = len.to_s.size + 1
183
+ i = 1
184
+ seq.gsub!(/^/) { |x| s = sprintf("\n%*d ", w, i); i += 50; s }
185
+
186
+ [ head, "\n", definition, "\n\n",
187
+ "#{entry_id} Length: #{len} #{date} " \
188
+ "Type: #{seq_type} Check: #{checksum} ..\n",
189
+ seq, "\n" ].join('')
190
+ end
191
+
192
+ end #class Seq
193
+ end #module GCG
194
+ end #module Bio
195
+
@@ -0,0 +1,552 @@
1
+ #
2
+ # = bio/appl/genscan/report.rb - Genscan report classes
3
+ #
4
+ # Copyright:: Copyright (C) 2003
5
+ # Mitsuteru C. Nakao <n@bioruby.org>
6
+ # License:: The Ruby License
7
+ #
8
+ # $Id:$
9
+ #
10
+ # == Description
11
+ #
12
+ #
13
+ # == Example
14
+ #
15
+ # == References
16
+ #
17
+
18
+ require 'bio/db/fasta'
19
+
20
+
21
+ module Bio
22
+
23
+ # = Bio::Genscan
24
+ class Genscan
25
+
26
+ # = Bio::Genscan::Report - Class for Genscan report output.
27
+ #
28
+ # Parser for the Genscan report output.
29
+ # * Genscan http://genes.mit.edu/GENSCAN.html
30
+ class Report
31
+
32
+ # Returns Genscan version.
33
+ attr_reader :genscan_version
34
+
35
+ # Returns
36
+ attr_reader :date_run
37
+
38
+ # Returns
39
+ attr_reader :time
40
+
41
+ # Returns Name of query sequence.
42
+ attr_reader :query_name
43
+ alias_method :sequence_name, :query_name
44
+ alias_method :name, :query_name
45
+
46
+ # Returns Length of the query sequence.
47
+ attr_reader :length
48
+
49
+ # Returns C+G content of the query sequence.
50
+ attr_reader :gccontent
51
+
52
+ # Returns
53
+ attr_reader :isochore
54
+
55
+ # Returns
56
+ attr_reader :matrix
57
+
58
+ # Returns Array of Bio::Genscan::Report::Gene.
59
+ attr_reader :predictions
60
+ alias_method :prediction, :predictions
61
+ alias_method :genes, :predictions
62
+
63
+
64
+ # Bio::Genscan::Report.new(str)
65
+ #
66
+ # Parse a Genscan report output string.
67
+ def initialize(report)
68
+ @predictions = []
69
+ @genscan_version = nil
70
+ @date_run = nil
71
+ @time = nil
72
+ @query_name = nil
73
+ @length = nil
74
+ @gccontent = nil
75
+ @isochore = nil
76
+ @matrix = nil
77
+
78
+ report.each_line("\n") do |line|
79
+ case line
80
+ when /^GENSCAN/
81
+ parse_headline(line)
82
+ when /^Sequence/
83
+ parse_sequence(line)
84
+ when /^Parameter/
85
+ parse_parameter(line)
86
+ when /^Predicted genes/
87
+ break
88
+ end
89
+ end
90
+
91
+ # rests
92
+ i = report.index(/^Predicted gene/)
93
+ j = report.index(/^Predicted peptide sequence/)
94
+
95
+ # genes/exons
96
+ genes_region = report[i...j]
97
+ genes_region.each_line("\n") do |line|
98
+ if /Init|Intr|Term|PlyA|Prom|Sngl/ =~ line
99
+ gn, en = line.strip.split(" +")[0].split(/\./).map {|i| i.to_i }
100
+ add_exon(gn, en, line)
101
+ end
102
+ end
103
+
104
+ # sequences (peptide|CDS)
105
+ sequence_region = report[j...report.size]
106
+ sequence_region.gsub!(/^Predicted .+?:/, '')
107
+ sequence_region.gsub!(/^\s*$/, '')
108
+ sequence_region.split(Bio::FastaFormat::RS).each do |ff|
109
+ add_seq(Bio::FastaFormat.new(ff))
110
+ end
111
+ end
112
+
113
+
114
+ # Bio::Genscan::Report#parse_headline
115
+ def parse_headline(line)
116
+ tmp = line.chomp.split(/\t/)
117
+ @genscan_version = tmp[0].split(' ')[1]
118
+ @date_run = tmp[1].split(': ')[1]
119
+ @time = tmp[2].split(': ')[1]
120
+ end
121
+ private :parse_headline
122
+
123
+
124
+ # Bio::Genscan::Report#parse_sequence
125
+ def parse_sequence(line)
126
+ if /^Sequence (\S+) : (\d+) bp : (\d+[\.\d]+)% C\+G : Isochore (\d+.+?)$/ =~ line
127
+ @query_name = $1
128
+ @length = $2.to_i
129
+ @gccontent = $3.to_f
130
+ @isochore = $4
131
+ else
132
+ raise "Error: [#{line.inspect}]"
133
+ end
134
+ end
135
+ private :parse_sequence
136
+
137
+
138
+ # Bio::Genscan::Report#parse_parameter
139
+ def parse_parameter(line)
140
+ if /^Parameter matrix: (\w.+)$/ =~ line.chomp
141
+ @matrix = $1
142
+ else
143
+ raise "Error: [#{line}]"
144
+ end
145
+ end
146
+ private :parse_parameter
147
+
148
+
149
+ # Bio::Genscan::Report#add_gene
150
+ def add_gene(gn)
151
+ @predictions[gn - 1] = Gene.new(gn)
152
+ end
153
+ private :add_gene
154
+
155
+
156
+ # Bio::Genscan::Report#add_exon
157
+ def add_exon(gn, en, line)
158
+ exon = Exon.parser(line)
159
+ case line
160
+ when /Prom/
161
+ begin
162
+ @predictions[gn - 1].set_promoter(exon)
163
+ rescue NameError
164
+ add_gene(gn)
165
+ @predictions[gn - 1].set_promoter(exon)
166
+ end
167
+ when /PlyA/
168
+ @predictions[gn - 1].set_polyA(exon)
169
+ else
170
+ begin
171
+ @predictions[gn - 1].exons[en - 1] = exon
172
+ rescue NameError
173
+ add_gene(gn)
174
+ @predictions[gn - 1].exons[en - 1] = exon
175
+ end
176
+ end
177
+ end
178
+ private :add_exon
179
+
180
+
181
+ # Bio::Genscan::Report#add_seq
182
+ def add_seq(seq)
183
+ if /peptide_(\d+)/ =~ seq.definition
184
+ gn = $1.to_i
185
+ @predictions[gn - 1].set_aaseq(seq)
186
+ elsif /CDS_(\d+)/ =~ seq.definition
187
+ gn = $1.to_i
188
+ @predictions[gn - 1].set_naseq(seq)
189
+ end
190
+ end
191
+ private :add_seq
192
+
193
+
194
+ # = Container class of predicted gene structures.
195
+ class Gene
196
+
197
+ # Bio::Genescan::Report::Gene.new(gene_number)
198
+ def initialize(gn)
199
+ @number = gn.to_i
200
+ @aaseq = Bio::FastaFormat.new("")
201
+ @naseq = Bio::FastaFormat.new("")
202
+ @promoter = nil
203
+ @exons = []
204
+ @polyA = nil
205
+ end
206
+
207
+ # Returns "Gn", gene number field.
208
+ attr_reader :number
209
+
210
+ # Returns Bio::FastaFormat object.
211
+ attr_reader :aaseq
212
+
213
+ # Returns Bio::FastaFormat object.
214
+ attr_reader :naseq
215
+
216
+ # Returns Array of Bio::Genscan::Report::Exon.
217
+ attr_reader :exons
218
+
219
+ # Returns Bio::Genscan::Report::Exon object.
220
+ attr_reader :promoter
221
+
222
+ # Returns Bio::Genscan::Report::Exon object.
223
+ attr_reader :polyA
224
+
225
+
226
+ # Bio::Genescan::Report::Gene#seq_aaseq
227
+ def set_aaseq(seq)
228
+ @aaseq = seq
229
+ end
230
+
231
+
232
+ # Bio::Genescan::Report::Gene#seq_naseq
233
+ def set_naseq(seq)
234
+ @naseq = seq
235
+ end
236
+
237
+
238
+ # Bio::Genescan::Report::Gene#seq_promoter
239
+ def set_promoter(segment)
240
+ @promoter = segment
241
+ end
242
+
243
+
244
+ # Bio::Genescan::Report::Gene#seq_polyA
245
+ def set_polyA(segment)
246
+ @polyA = segment
247
+ end
248
+
249
+ end # class Gene
250
+
251
+
252
+ # = Container class of a predicted gene structure.
253
+ class Exon
254
+
255
+ #
256
+ TYPES = {
257
+ 'Init' => 'Initial exon',
258
+ 'Intr' => 'Internal exon',
259
+ 'Term' => 'Terminal exon',
260
+ 'Sngl' => 'Single-exon gene',
261
+ 'Prom' => 'Promoter',
262
+ 'PlyA' => 'poly-A signal'
263
+ }
264
+
265
+
266
+ # Bio::Genescan::Report::Exon.parser
267
+ def self.parser(line)
268
+ e = line.strip.split(/ +/)
269
+ case line
270
+ when /PlyA/, /Prom/
271
+ e[12] = e[6].clone
272
+ e[11] = 0
273
+ [6,7,8,9,10].each {|i| e[i] = nil }
274
+ end
275
+ self.new(e[0], e[1], e[2], e[3], e[4], e[5], e[6],
276
+ e[7], e[8], e[9], e[10], e[11], e[12])
277
+ end
278
+
279
+
280
+ # Returns
281
+ attr_reader :gene_number
282
+
283
+ # Returns "Ex", exon number field
284
+ attr_reader :number
285
+
286
+ # Returns "Type" field.
287
+ attr_reader :exon_type
288
+
289
+ # Returns "S" field.
290
+ attr_reader :strand
291
+
292
+ # Returns Returns first position of the region. "Begin" field.
293
+ attr_reader :first
294
+
295
+ # Returns Returns last position of the region. "End" field.
296
+ attr_reader :last
297
+
298
+ # Returns "Fr" field.
299
+ attr_reader :frame
300
+
301
+ # Returns "Ph" field.
302
+ attr_reader :phase
303
+
304
+ # Returns "CodRg" field.
305
+ attr_reader :score
306
+
307
+ # Returns "P" field.
308
+ attr_reader :p_value
309
+
310
+ # Returns "Tscr" field.
311
+ attr_reader :t_score
312
+ alias_method :coding_region_score, :score
313
+
314
+
315
+ # Bio::Genescan::Report::Exon.new(gene_number, exon_type, strand, first,
316
+ # end, length, frame, phase, acceptor_score, donor_score, score, p_value,
317
+ # t_score)
318
+ def initialize(gnex, t, s, b, e, len, fr, ph, iac, dot, cr, prob, ts)
319
+ @gene_number, @number = gnex.split(".").map {|n| n.to_i }
320
+ @exon_type = t
321
+ @strand = s
322
+ @first = b.to_i
323
+ @last = e.to_i
324
+ @length = len.to_i
325
+ @frame = fr
326
+ @phase = ph
327
+ @i_ac = iac.to_i
328
+ @do_t = dot.to_i
329
+ @score = cr.to_i
330
+ @p_value = prob.to_f
331
+ @t_score = ts.to_f
332
+ end
333
+
334
+
335
+
336
+ # Bio::Genescan::Report::Exon#exon_type_long
337
+ #
338
+ # Returns a human-readable "Type" of exon.
339
+ def exon_type_long
340
+ TYPES[exon_type]
341
+ end
342
+
343
+
344
+ # Bio::Genescan::Report::Exon#range
345
+ #
346
+ # Returns Range object of the region.
347
+ def range
348
+ Range.new(@first, @last)
349
+ end
350
+
351
+
352
+ # Bio::Genescan::Report::Exon#acceptor_score
353
+ #
354
+ # "I/Ac" field.
355
+ def acceptor_score
356
+ @i_ac
357
+ end
358
+ alias_method :initiation_score, :acceptor_score
359
+
360
+
361
+ # Bio::Genescan::Report::Exon#donor_score
362
+ #
363
+ # "Do/T" field.
364
+ def donor_score
365
+ @do_t
366
+ end
367
+ alias_method :termination_score, :donor_score
368
+
369
+ end # class Exon
370
+
371
+ end # class Report
372
+
373
+ end # class Genscan
374
+
375
+ end # module Bio
376
+
377
+
378
+
379
+
380
+
381
+ # testing code
382
+
383
+ if __FILE__ == $0
384
+
385
+ if $<.filename != '-'
386
+ report = $<.read
387
+ else
388
+ report = File.open(__FILE__, 'r').read.scan(/^>>>> (.+)$/).join("\n")
389
+ end
390
+
391
+
392
+ puts "= class Bio::Genscan::Report "
393
+ report = Bio::Genscan::Report.new(report)
394
+
395
+
396
+ print " report.genscan_version #=> "
397
+ p report.genscan_version
398
+ print " report.date_run #=> "
399
+ p report.date_run
400
+ print " report.time #=> "
401
+ p report.time
402
+
403
+ print " report.query_name #=> "
404
+ p report.query_name
405
+ print " report.length #=> "
406
+ p report.length
407
+ print " report.gccontent #=> "
408
+ p report.gccontent
409
+ print " report.isochore #=> "
410
+ p report.isochore
411
+
412
+ print " report.matrix #=> "
413
+ p report.matrix
414
+
415
+ puts " report.predictions (Array of Bio::Genscan::Report::Gene) "
416
+ print " report.predictions.size #=> "
417
+ p report.predictions.size
418
+
419
+
420
+ report.predictions.each {|gene|
421
+ puts "\n== class Bio::Genscan::Report::Gene "
422
+ print " gene.number #=> "
423
+ p gene.number
424
+ print " gene.aaseq (Bio::FastaFormat) #=> "
425
+ p gene.aaseq
426
+ print " gene.naseq (Bio::FastaFormat) #=> "
427
+ p gene.naseq
428
+ print " ene.promoter (Bio::Genscan::Report::Exon) #=> "
429
+ p gene.promoter
430
+ print " gene.polyA (Bio::Genscan::Report::Exon) #=> "
431
+ p gene.polyA
432
+ puts " gene.exons (Array of Bio::Genscan::Report::Exon) "
433
+ print " gene.exons.size #=> "
434
+ p gene.exons.size
435
+
436
+
437
+ gene.exons.each {|exon|
438
+ puts "\n== class Bio::Genscan::Report::Exon "
439
+ print " exon.number #=> "
440
+ p exon.number
441
+ print " exon.exon_type #=> "
442
+ p exon.exon_type
443
+ print " exon.exon_type_long #=> "
444
+ p exon.exon_type_long
445
+ print " exon.strand #=> "
446
+ p exon.strand
447
+ print " exon.first #=> "
448
+ p exon.first
449
+ print " exon.last #=> "
450
+ p exon.last
451
+ print " exon.range (Range) #=> "
452
+ p exon.range
453
+ print " exon.frame #=> "
454
+ p exon.frame
455
+ print " exon.phase #=> "
456
+ p exon.phase
457
+ print " exon.acceptor_score #=> "
458
+ p exon.acceptor_score
459
+ print " exon.donor_score #=> "
460
+ p exon.donor_score
461
+ print " exon.initiation_score #=> "
462
+ p exon.initiation_score
463
+ print " exon.termination_score #=> "
464
+ p exon.termination_score
465
+ print " exon.score #=> "
466
+ p exon.score
467
+ print " exon.p_value #=> "
468
+ p exon.p_value
469
+ print " exon.t_score #=> "
470
+ p exon.t_score
471
+ puts
472
+ }
473
+ puts
474
+ }
475
+
476
+ end
477
+
478
+
479
+
480
+
481
+
482
+ =begin
483
+
484
+
485
+ = Sample Genscan report with '^>>>> '.
486
+
487
+
488
+ >>>> GENSCAN 1.0 Date run: 30-May-103 Time: 14:06:28
489
+ >>>>
490
+ >>>> Sequence HUMRASH : 12942 bp : 68.17% C+G : Isochore 4 (57 - 100 C+G%)
491
+ >>>>
492
+ >>>> Parameter matrix: HumanIso.smat
493
+ >>>>
494
+ >>>> Predicted genes/exons:
495
+ >>>>
496
+ >>>> Gn.Ex Type S .Begin ...End .Len Fr Ph I/Ac Do/T CodRg P.... Tscr..
497
+ >>>> ----- ---- - ------ ------ ---- -- -- ---- ---- ----- ----- ------
498
+ >>>>
499
+ >>>> 1.01 Init + 1664 1774 111 1 0 94 83 212 0.997 21.33
500
+ >>>> 1.02 Intr + 2042 2220 179 1 2 104 66 408 0.997 40.12
501
+ >>>> 1.03 Intr + 2374 2533 160 1 1 89 94 302 0.999 32.08
502
+ >>>> 1.04 Term + 3231 3350 120 2 0 115 48 202 0.980 18.31
503
+ >>>> 1.05 PlyA + 3722 3727 6 -5.80
504
+ >>>>
505
+ >>>> 2.00 Prom + 6469 6508 40 -7.92
506
+ >>>> 2.01 Init + 8153 8263 111 1 0 94 83 212 0.998 21.33
507
+ >>>> 2.02 Intr + 8531 8709 179 1 2 104 66 408 0.997 40.12
508
+ >>>> 2.03 Intr + 8863 9022 160 1 1 89 94 302 0.999 32.08
509
+ >>>> 2.04 Term + 9720 9839 120 2 0 115 48 202 0.961 18.31
510
+ >>>>
511
+ >>>> Predicted peptide sequence(s):
512
+ >>>>
513
+ >>>> Predicted coding sequence(s):
514
+ >>>>
515
+ >>>>
516
+ >>>> >HUMRASH|GENSCAN_predicted_peptide_1|189_aa
517
+ >>>> MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAG
518
+ >>>> QEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVKDSDDVPMVLVGNKCDL
519
+ >>>> AARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREIRQHKLRKLNPPDESGPG
520
+ >>>> CMSCKCVLS
521
+ >>>>
522
+ >>>> >HUMRASH|GENSCAN_predicted_CDS_1|570_bp
523
+ >>>> atgacggaatataagctggtggtggtgggcgccggcggtgtgggcaagagtgcgctgacc
524
+ >>>> atccagctgatccagaaccattttgtggacgaatacgaccccactatagaggattcctac
525
+ >>>> cggaagcaggtggtcattgatggggagacgtgcctgttggacatcctggataccgccggc
526
+ >>>> caggaggagtacagcgccatgcgggaccagtacatgcgcaccggggagggcttcctgtgt
527
+ >>>> gtgtttgccatcaacaacaccaagtcttttgaggacatccaccagtacagggagcagatc
528
+ >>>> aaacgggtgaaggactcggatgacgtgcccatggtgctggtggggaacaagtgtgacctg
529
+ >>>> gctgcacgcactgtggaatctcggcaggctcaggacctcgcccgaagctacggcatcccc
530
+ >>>> tacatcgagacctcggccaagacccggcagggagtggaggatgccttctacacgttggtg
531
+ >>>> cgtgagatccggcagcacaagctgcggaagctgaaccctcctgatgagagtggccccggc
532
+ >>>> tgcatgagctgcaagtgtgtgctctcctga
533
+ >>>>
534
+ >>>> >HUMRASH|GENSCAN_predicted_peptide_2|189_aa
535
+ >>>> MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAG
536
+ >>>> QEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVKDSDDVPMVLVGNKCDL
537
+ >>>> AARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREIRQHKLRKLNPPDESGPG
538
+ >>>> CMSCKCVLS
539
+ >>>>
540
+ >>>> >HUMRASH|GENSCAN_predicted_CDS_2|570_bp
541
+ >>>> atgacggaatataagctggtggtggtgggcgccggcggtgtgggcaagagtgcgctgacc
542
+ >>>> atccagctgatccagaaccattttgtggacgaatacgaccccactatagaggattcctac
543
+ >>>> cggaagcaggtggtcattgatggggagacgtgcctgttggacatcctggataccgccggc
544
+ >>>> caggaggagtacagcgccatgcgggaccagtacatgcgcaccggggagggcttcctgtgt
545
+ >>>> gtgtttgccatcaacaacaccaagtcttttgaggacatccaccagtacagggagcagatc
546
+ >>>> aaacgggtgaaggactcggatgacgtgcccatggtgctggtggggaacaagtgtgacctg
547
+ >>>> gctgcacgcactgtggaatctcggcaggctcaggacctcgcccgaagctacggcatcccc
548
+ >>>> tacatcgagacctcggccaagacccggcagggagtggaggatgccttctacacgttggtg
549
+ >>>> cgtgagatccggcagcacaagctgcggaagctgaaccctcctgatgagagtggccccggc
550
+ >>>> tgcatgagctgcaagtgtgtgctctcctga
551
+
552
+ =end