genevalidator 1.6.1 → 1.6.2
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- checksums.yaml +4 -4
- data/.gitignore +3 -1
- data/.travis.yml +2 -0
- data/README.md +78 -30
- data/Rakefile +11 -8
- data/aux/app_template_footer.erb +1 -6
- data/aux/app_template_header.erb +12 -32
- data/aux/files/css/style.css +2 -8
- data/aux/files/js/plots.js +564 -576
- data/aux/files/js/script.js +10 -0
- data/aux/json_footer.erb +8 -0
- data/aux/json_header.erb +19 -0
- data/aux/json_query.erb +14 -0
- data/aux/template_footer.erb +9 -58
- data/aux/template_header.erb +18 -58
- data/aux/template_query.erb +8 -36
- data/bin/genevalidator +45 -32
- data/genevalidator.gemspec +11 -7
- data/lib/genevalidator.rb +75 -455
- data/lib/genevalidator/arg_validation.rb +78 -107
- data/lib/genevalidator/blast.rb +57 -60
- data/lib/genevalidator/clusterization.rb +15 -15
- data/lib/genevalidator/exceptions.rb +32 -5
- data/lib/genevalidator/get_raw_sequences.rb +70 -33
- data/lib/genevalidator/hsp.rb +1 -4
- data/lib/genevalidator/json_to_gv_results.rb +109 -0
- data/lib/genevalidator/output.rb +177 -185
- data/lib/genevalidator/pool.rb +2 -1
- data/lib/genevalidator/sequences.rb +3 -3
- data/lib/genevalidator/tabular_parser.rb +24 -18
- data/lib/genevalidator/validation.rb +279 -0
- data/lib/genevalidator/validation_alignment.rb +31 -47
- data/lib/genevalidator/validation_blast_reading_frame.rb +19 -18
- data/lib/genevalidator/validation_duplication.rb +23 -19
- data/lib/genevalidator/validation_gene_merge.rb +30 -65
- data/lib/genevalidator/validation_length_cluster.rb +14 -53
- data/lib/genevalidator/validation_length_rank.rb +10 -11
- data/lib/genevalidator/validation_open_reading_frame.rb +18 -19
- data/lib/genevalidator/validation_report.rb +2 -5
- data/lib/genevalidator/validation_test.rb +8 -4
- data/lib/genevalidator/version.rb +1 -1
- data/test/test_all_validations.rb +51 -66
- data/test/test_blast.rb +68 -51
- data/test/test_clusterization.rb +1 -1
- data/test/test_clusterization_2d.rb +19 -13
- data/test/test_extended_array_methods.rb +1 -1
- data/test/test_files/all_validations_mrna/mrna.blast_tab6 +1806 -0
- data/test/test_files/all_validations_mrna/mrna.blast_tab7 +1865 -0
- data/test/test_files/all_validations_mrna/{all_validations_mrna.fasta.blast_xml → mrna.blast_xml} +18642 -1
- data/test/test_files/all_validations_mrna/{all_validations_mrna.fasta.blast_xml.index → mrna.blast_xml.index} +300 -0
- data/test/test_files/all_validations_mrna/{all_validations_mrna.fasta → mrna.fa} +0 -0
- data/test/test_files/all_validations_mrna/mrna.raw_seq +3970 -0
- data/test/test_files/all_validations_mrna/{all_validations_mrna.fasta.blast_xml.raw_seq.idx → mrna.raw_seq.idx} +901 -1
- data/test/test_files/all_validations_prot/{all_validations_prot.fasta.blast_tab → prot.blast_tab6} +416 -0
- data/test/test_files/all_validations_prot/prot.blast_tab7 +2400 -0
- data/test/test_files/all_validations_prot/{all_validations_prot.fasta.blast_xml → prot.blast_xml} +18299 -6723
- data/test/test_files/all_validations_prot/{all_validations_prot.fasta.blast_xml.index → prot.blast_xml.index} +408 -0
- data/test/test_files/all_validations_prot/{all_validations_prot.fasta → prot.fa} +0 -0
- data/test/test_files/all_validations_prot/{all_validations_prot.fasta.blast_xml.raw_seq → prot.raw_seq} +2735 -0
- data/test/test_files/all_validations_prot/{all_validations_prot.fasta.blast_xml.raw_seq.idx → prot.raw_seq.idx} +3032 -1808
- data/test/test_sequences.rb +46 -41
- data/test/test_validation_open_reading_frame.rb +318 -202
- data/test/test_validations.rb +48 -32
- metadata +76 -102
- data/doc/AliasDuplicationError.html +0 -134
- data/doc/AlignmentValidation.html +0 -1687
- data/doc/AlignmentValidationOutput.html +0 -659
- data/doc/Blast.html +0 -1905
- data/doc/BlastRFValidationOutput.html +0 -545
- data/doc/BlastReadingFrameValidation.html +0 -370
- data/doc/BlastUtils.html +0 -875
- data/doc/ClasspathError.html +0 -134
- data/doc/Cluster.html +0 -1316
- data/doc/DuplciationValidationOutput.html +0 -564
- data/doc/DuplicationValidation.html +0 -920
- data/doc/DuplicationValidationOutput.html +0 -564
- data/doc/FileNotFoundException.html +0 -134
- data/doc/GeneMergeValidation.html +0 -935
- data/doc/GeneMergeValidationOutput.html +0 -652
- data/doc/HierarchicalClusterization.html +0 -994
- data/doc/Hsp.html +0 -1485
- data/doc/InconsistentTabularFormat.html +0 -135
- data/doc/LengthClusterValidation.html +0 -982
- data/doc/LengthClusterValidationOutput.html +0 -515
- data/doc/LengthRankValidation.html +0 -496
- data/doc/LengthRankValidationOutput.html +0 -517
- data/doc/NoInternetError.html +0 -135
- data/doc/NoMafftInstallationError.html +0 -134
- data/doc/NoPIdentError.html +0 -134
- data/doc/NoValidationError.html +0 -134
- data/doc/NotEnoughHitsError.html +0 -135
- data/doc/ORFValidationOutput.html +0 -593
- data/doc/OpenReadingFrameValidation.html +0 -1107
- data/doc/OtherError.html +0 -123
- data/doc/Output.html +0 -1540
- data/doc/Pair.html +0 -309
- data/doc/PairCluster.html +0 -767
- data/doc/Plot.html +0 -837
- data/doc/QueryError.html +0 -134
- data/doc/ReportClassError.html +0 -135
- data/doc/Sequence.html +0 -1299
- data/doc/SequenceTypeError.html +0 -135
- data/doc/TabularEntry.html +0 -837
- data/doc/TabularParser.html +0 -1104
- data/doc/Validation.html +0 -2147
- data/doc/ValidationClassError.html +0 -134
- data/doc/ValidationOutput.html +0 -460
- data/doc/ValidationReport.html +0 -940
- data/doc/ValidationTest.html +0 -939
- data/doc/_index.html +0 -449
- data/doc/class_list.html +0 -54
- data/doc/css/common.css +0 -1
- data/doc/css/full_list.css +0 -57
- data/doc/css/style.css +0 -338
- data/doc/file.README.html +0 -151
- data/doc/file_list.html +0 -56
- data/doc/frames.html +0 -26
- data/doc/index.html +0 -151
- data/doc/js/app.js +0 -214
- data/doc/js/full_list.js +0 -178
- data/doc/js/jquery.js +0 -4
- data/doc/method_list.html +0 -1505
- data/doc/top-level-namespace.html +0 -112
- data/test/test_files/all_validations_mrna/all_validations_mrna.fasta.blast_tab +0 -967
- data/test/test_files/all_validations_mrna/all_validations_mrna.fasta.blast_tab.index +0 -967
- data/test/test_files/all_validations_mrna/all_validations_mrna.fasta.blast_tab.raw_seq +0 -4929
- data/test/test_files/all_validations_mrna/all_validations_mrna.fasta.blast_tab.raw_seq.idx +0 -1006
- data/test/test_files/all_validations_mrna/all_validations_mrna.fasta.blast_xml.raw_seq +0 -2075
- data/test/test_files/all_validations_prot/all_validations_prot.fasta.blast_tab.index +0 -1864
- data/test/test_files/all_validations_prot/all_validations_prot.fasta.blast_tab.raw_seq +0 -42411
- data/test/test_files/all_validations_prot/all_validations_prot.fasta.blast_tab.raw_seq.idx +0 -3751
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— Documentation by YARD 0.8.7.2
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<div id="content"><div id='filecontents'><h1>Identify problems with predicted genes</h1>
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<p>This is a GSoC 2013 project.<br>
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Details about the project's progress during the <strong>Coding period</strong> can be found <a href="https://github.com/monicadragan/gene_prediction/wiki/Project-Diary">here</a>.<br>
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We also have a <a href="http://gene-prediction.blogspot.ro/">blog</a>.<br><br>
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<h3><div align = center><font color="blue">Please note that some of the functionalities<br> of this tool are still under development.<br> So, stay tunned!</font></div></h3>
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<br><br></p>
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<h3>Authors</h3>
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<ul>
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<li>GSoC student: <a href="http://swarm.cs.pub.ro/%7Emdragan/gsoc2013/Monica_Dragan_CV.pdf">Monica Dragan</a> (<a href="mailto:monica.dragan@cti.pub.ro">email</a>)</li>
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<li>Mentors: <a href="https://plus.google.com/114122400102590087616/about">Anurag Priyam</a>(<a href="mailto:anurag08priyam@gmail.com">email</a>) and <a href="http://yannick.poulet.org/">Yannick Wurm</a>(<a href="mailto:y.wurm@qmul.ac.uk">email</a>)</li>
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<h3>Abstract</h3>
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<p>The goal of GeneValidator is to identify problems with gene predictions and provide useful information based on the similarities to genes in public databases.The results of the prediction validation will make evidence about how the sequencing curation may be done and can be useful in improving / trying new approaches for gene prediction tools. The main target users of this tool are the Biologists who want to validate the data obtained in their own laboratories.</p>
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<h3>Current Validations</h3>
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<li>Length validation by clusterization</li>
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<li>Reading frame validation</li>
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<li>Check gene merge</li>
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<li>Check duplications</li>
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<li>Main ORF validation (for nucleotides)</li>
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<li>Validation based on multiple alignment ~ under development</li>
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<li>Codon coverage ~ under development</li>
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<h3>Requirements</h3>
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<li>Ruby (>= 1.9.3)</li>
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<li>RubyGems (>= 1.3.6)</li>
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<li>NCBI BLAST+ (>= 2.2.25+)</li>
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<li>MAFFT installation (download it from : <a href="http://mafft.cbrc.jp/alignment/software/">http://mafft.cbrc.jp/alignment/software/</a> ).<br>
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Linux and MacOS are officially supported!</li>
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<h3>Installation</h3>
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<li><p>Get the source code<br>
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<code>$ git clone git@github.com:monicadragan/gene_prediction.git</code></p></li>
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<li><p>Be sudo and build the gem<br>
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<code>$ sudo rake</code></p></li>
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<li><p>Run GeneValidation<br>
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<code>$ genevalidator [validations] [skip_blast] [start] [tabular] [mafft] [raw_seq] FILE</code> </p></li>
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<p>Example that emphasizes all the validations:<br>
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<code>$ genevalidator -x data/all_validations_prot/all_validations_prot.xml data/all_validations_prot/all_validations_prot.fasta</code></p>
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<p>Learn more:<br>
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<code>$ genevalidator -h</code></p>
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<h3>Outputs</h3>
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<p>By running GeneValidator on your dataset you get numbers and plots. Some relevant files will be generated at the same path with the input file. The results are available in 3 formats:
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* console table output
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* validation results in YAML format (the YAML file has the same name with the input file + YAML extension)
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* html output with plot visualization (the useful files will be generated in the 'html' directory, at the same path with the input file)<br>
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! Note: for the moment check the html output with Firefox browser only !</p>
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<p><a href="http://swarm.cs.pub.ro/%7Emdragan/gsoc2013/genevalidator">Have a look at our results!</a></p>
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<h3>Other things</h3>
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<ol>
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<li><p>Run unit tests<br>
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<code>$ rake test</code></p></li>
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<li><p>Generate documentation<br>
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<code>$ rake doc</code></p></li>
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</ol>
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</div></div>
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<div id="footer">
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Generated on Sat Sep 28 07:01:32 2013 by
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<a href="http://yardoc.org" title="Yay! A Ruby Documentation Tool" target="_parent">yard</a>
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0.8.7.2 (ruby-1.9.3).
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</body>
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</html>
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data/doc/js/app.js
DELETED
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function createSourceLinks() {
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2
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$('.method_details_list .source_code').
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before("<span class='showSource'>[<a href='#' class='toggleSource'>View source</a>]</span>");
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4
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$('.toggleSource').toggle(function() {
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$(this).parent().nextAll('.source_code').slideDown(100);
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$(this).text("Hide source");
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},
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function() {
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$(this).parent().nextAll('.source_code').slideUp(100);
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$(this).text("View source");
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11
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});
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12
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}
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13
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-
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14
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-
function createDefineLinks() {
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15
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-
var tHeight = 0;
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16
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$('.defines').after(" <a href='#' class='toggleDefines'>more...</a>");
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$('.toggleDefines').toggle(function() {
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tHeight = $(this).parent().prev().height();
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$(this).prev().show();
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$(this).parent().prev().height($(this).parent().height());
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$(this).text("(less)");
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},
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function() {
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$(this).prev().hide();
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$(this).parent().prev().height(tHeight);
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$(this).text("more...");
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27
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});
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28
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}
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function createFullTreeLinks() {
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var tHeight = 0;
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$('.inheritanceTree').toggle(function() {
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tHeight = $(this).parent().prev().height();
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$(this).parent().toggleClass('showAll');
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$(this).text("(hide)");
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$(this).parent().prev().height($(this).parent().height());
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},
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function() {
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$(this).parent().toggleClass('showAll');
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$(this).parent().prev().height(tHeight);
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$(this).text("show all");
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});
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}
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function fixBoxInfoHeights() {
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$('dl.box dd.r1, dl.box dd.r2').each(function() {
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$(this).prev().height($(this).height());
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});
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}
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|
51
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function searchFrameLinks() {
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52
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$('.full_list_link').click(function() {
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toggleSearchFrame(this, $(this).attr('href'));
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return false;
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});
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}
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function toggleSearchFrame(id, link) {
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var frame = $('#search_frame');
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$('#search a').removeClass('active').addClass('inactive');
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if (frame.attr('src') == link && frame.css('display') != "none") {
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frame.slideUp(100);
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$('#search a').removeClass('active inactive');
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}
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else {
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$(id).addClass('active').removeClass('inactive');
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frame.attr('src', link).slideDown(100);
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}
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}
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71
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function linkSummaries() {
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$('.summary_signature').click(function() {
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document.location = $(this).find('a').attr('href');
|
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});
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}
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function framesInit() {
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if (hasFrames) {
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document.body.className = 'frames';
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$('#menu .noframes a').attr('href', document.location);
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window.top.document.title = $('html head title').text();
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}
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else {
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$('#menu .noframes a').text('frames').attr('href', framesUrl);
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}
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}
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function keyboardShortcuts() {
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$(document).keypress(function(evt) {
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if (evt.altKey || evt.ctrlKey || evt.metaKey || evt.shiftKey) return;
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if (typeof evt.target !== "undefined" &&
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(evt.target.nodeName == "INPUT" ||
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evt.target.nodeName == "TEXTAREA")) return;
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switch (evt.charCode) {
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case 67: case 99: $('#class_list_link').click(); break; // 'c'
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case 77: case 109: $('#method_list_link').click(); break; // 'm'
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case 70: case 102: $('#file_list_link').click(); break; // 'f'
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default: break;
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}
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});
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}
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function summaryToggle() {
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$('.summary_toggle').click(function() {
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if (localStorage) {
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localStorage.summaryCollapsed = $(this).text();
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}
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$('.summary_toggle').each(function() {
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$(this).text($(this).text() == "collapse" ? "expand" : "collapse");
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var next = $(this).parent().parent().nextAll('ul.summary').first();
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if (next.hasClass('compact')) {
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next.toggle();
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next.nextAll('ul.summary').first().toggle();
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}
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else if (next.hasClass('summary')) {
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var list = $('<ul class="summary compact" />');
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list.html(next.html());
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list.find('.summary_desc, .note').remove();
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list.find('a').each(function() {
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$(this).html($(this).find('strong').html());
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$(this).parent().html($(this)[0].outerHTML);
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});
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next.before(list);
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next.toggle();
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}
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});
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return false;
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});
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if (localStorage) {
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if (localStorage.summaryCollapsed == "collapse") {
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$('.summary_toggle').first().click();
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else localStorage.summaryCollapsed = "expand";
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}
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}
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function fixOutsideWorldLinks() {
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$('a').each(function() {
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if (window.location.host != this.host) this.target = '_parent';
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});
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}
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function generateTOC() {
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if ($('#filecontents').length === 0) return;
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var _toc = $('<ol class="top"></ol>');
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var show = false;
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var toc = _toc;
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var counter = 0;
|
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var tags = ['h2', 'h3', 'h4', 'h5', 'h6'];
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var i;
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if ($('#filecontents h1').length > 1) tags.unshift('h1');
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for (i = 0; i < tags.length; i++) { tags[i] = '#filecontents ' + tags[i]; }
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var lastTag = parseInt(tags[0][1], 10);
|
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$(tags.join(', ')).each(function() {
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if ($(this).parents('.method_details .docstring').length != 0) return;
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if (this.id == "filecontents") return;
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show = true;
|
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var thisTag = parseInt(this.tagName[1], 10);
|
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if (this.id.length === 0) {
|
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var proposedId = $(this).attr('toc-id');
|
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if (typeof(proposedId) != "undefined") this.id = proposedId;
|
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else {
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var proposedId = $(this).text().replace(/[^a-z0-9-]/ig, '_');
|
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if ($('#' + proposedId).length > 0) { proposedId += counter; counter++; }
|
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|
-
this.id = proposedId;
|
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|
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}
|
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|
-
}
|
169
|
-
if (thisTag > lastTag) {
|
170
|
-
for (i = 0; i < thisTag - lastTag; i++) {
|
171
|
-
var tmp = $('<ol/>'); toc.append(tmp); toc = tmp;
|
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|
-
}
|
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|
-
}
|
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if (thisTag < lastTag) {
|
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|
-
for (i = 0; i < lastTag - thisTag; i++) toc = toc.parent();
|
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|
-
}
|
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|
-
var title = $(this).attr('toc-title');
|
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|
-
if (typeof(title) == "undefined") title = $(this).text();
|
179
|
-
toc.append('<li><a href="#' + this.id + '">' + title + '</a></li>');
|
180
|
-
lastTag = thisTag;
|
181
|
-
});
|
182
|
-
if (!show) return;
|
183
|
-
html = '<div id="toc"><p class="title"><a class="hide_toc" href="#"><strong>Table of Contents</strong></a> <small>(<a href="#" class="float_toc">left</a>)</small></p></div>';
|
184
|
-
$('#content').prepend(html);
|
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|
-
$('#toc').append(_toc);
|
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|
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$('#toc .hide_toc').toggle(function() {
|
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$('#toc .top').slideUp('fast');
|
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|
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$('#toc').toggleClass('hidden');
|
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|
-
$('#toc .title small').toggle();
|
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|
-
}, function() {
|
191
|
-
$('#toc .top').slideDown('fast');
|
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|
-
$('#toc').toggleClass('hidden');
|
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|
-
$('#toc .title small').toggle();
|
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|
-
});
|
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|
-
$('#toc .float_toc').toggle(function() {
|
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|
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$(this).text('float');
|
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|
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$('#toc').toggleClass('nofloat');
|
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|
-
}, function() {
|
199
|
-
$(this).text('left');
|
200
|
-
$('#toc').toggleClass('nofloat');
|
201
|
-
});
|
202
|
-
}
|
203
|
-
|
204
|
-
$(framesInit);
|
205
|
-
$(createSourceLinks);
|
206
|
-
$(createDefineLinks);
|
207
|
-
$(createFullTreeLinks);
|
208
|
-
$(fixBoxInfoHeights);
|
209
|
-
$(searchFrameLinks);
|
210
|
-
$(linkSummaries);
|
211
|
-
$(keyboardShortcuts);
|
212
|
-
$(summaryToggle);
|
213
|
-
$(fixOutsideWorldLinks);
|
214
|
-
$(generateTOC);
|