genevalidator 1.6.1 → 1.6.2
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- checksums.yaml +4 -4
- data/.gitignore +3 -1
- data/.travis.yml +2 -0
- data/README.md +78 -30
- data/Rakefile +11 -8
- data/aux/app_template_footer.erb +1 -6
- data/aux/app_template_header.erb +12 -32
- data/aux/files/css/style.css +2 -8
- data/aux/files/js/plots.js +564 -576
- data/aux/files/js/script.js +10 -0
- data/aux/json_footer.erb +8 -0
- data/aux/json_header.erb +19 -0
- data/aux/json_query.erb +14 -0
- data/aux/template_footer.erb +9 -58
- data/aux/template_header.erb +18 -58
- data/aux/template_query.erb +8 -36
- data/bin/genevalidator +45 -32
- data/genevalidator.gemspec +11 -7
- data/lib/genevalidator.rb +75 -455
- data/lib/genevalidator/arg_validation.rb +78 -107
- data/lib/genevalidator/blast.rb +57 -60
- data/lib/genevalidator/clusterization.rb +15 -15
- data/lib/genevalidator/exceptions.rb +32 -5
- data/lib/genevalidator/get_raw_sequences.rb +70 -33
- data/lib/genevalidator/hsp.rb +1 -4
- data/lib/genevalidator/json_to_gv_results.rb +109 -0
- data/lib/genevalidator/output.rb +177 -185
- data/lib/genevalidator/pool.rb +2 -1
- data/lib/genevalidator/sequences.rb +3 -3
- data/lib/genevalidator/tabular_parser.rb +24 -18
- data/lib/genevalidator/validation.rb +279 -0
- data/lib/genevalidator/validation_alignment.rb +31 -47
- data/lib/genevalidator/validation_blast_reading_frame.rb +19 -18
- data/lib/genevalidator/validation_duplication.rb +23 -19
- data/lib/genevalidator/validation_gene_merge.rb +30 -65
- data/lib/genevalidator/validation_length_cluster.rb +14 -53
- data/lib/genevalidator/validation_length_rank.rb +10 -11
- data/lib/genevalidator/validation_open_reading_frame.rb +18 -19
- data/lib/genevalidator/validation_report.rb +2 -5
- data/lib/genevalidator/validation_test.rb +8 -4
- data/lib/genevalidator/version.rb +1 -1
- data/test/test_all_validations.rb +51 -66
- data/test/test_blast.rb +68 -51
- data/test/test_clusterization.rb +1 -1
- data/test/test_clusterization_2d.rb +19 -13
- data/test/test_extended_array_methods.rb +1 -1
- data/test/test_files/all_validations_mrna/mrna.blast_tab6 +1806 -0
- data/test/test_files/all_validations_mrna/mrna.blast_tab7 +1865 -0
- data/test/test_files/all_validations_mrna/{all_validations_mrna.fasta.blast_xml → mrna.blast_xml} +18642 -1
- data/test/test_files/all_validations_mrna/{all_validations_mrna.fasta.blast_xml.index → mrna.blast_xml.index} +300 -0
- data/test/test_files/all_validations_mrna/{all_validations_mrna.fasta → mrna.fa} +0 -0
- data/test/test_files/all_validations_mrna/mrna.raw_seq +3970 -0
- data/test/test_files/all_validations_mrna/{all_validations_mrna.fasta.blast_xml.raw_seq.idx → mrna.raw_seq.idx} +901 -1
- data/test/test_files/all_validations_prot/{all_validations_prot.fasta.blast_tab → prot.blast_tab6} +416 -0
- data/test/test_files/all_validations_prot/prot.blast_tab7 +2400 -0
- data/test/test_files/all_validations_prot/{all_validations_prot.fasta.blast_xml → prot.blast_xml} +18299 -6723
- data/test/test_files/all_validations_prot/{all_validations_prot.fasta.blast_xml.index → prot.blast_xml.index} +408 -0
- data/test/test_files/all_validations_prot/{all_validations_prot.fasta → prot.fa} +0 -0
- data/test/test_files/all_validations_prot/{all_validations_prot.fasta.blast_xml.raw_seq → prot.raw_seq} +2735 -0
- data/test/test_files/all_validations_prot/{all_validations_prot.fasta.blast_xml.raw_seq.idx → prot.raw_seq.idx} +3032 -1808
- data/test/test_sequences.rb +46 -41
- data/test/test_validation_open_reading_frame.rb +318 -202
- data/test/test_validations.rb +48 -32
- metadata +76 -102
- data/doc/AliasDuplicationError.html +0 -134
- data/doc/AlignmentValidation.html +0 -1687
- data/doc/AlignmentValidationOutput.html +0 -659
- data/doc/Blast.html +0 -1905
- data/doc/BlastRFValidationOutput.html +0 -545
- data/doc/BlastReadingFrameValidation.html +0 -370
- data/doc/BlastUtils.html +0 -875
- data/doc/ClasspathError.html +0 -134
- data/doc/Cluster.html +0 -1316
- data/doc/DuplciationValidationOutput.html +0 -564
- data/doc/DuplicationValidation.html +0 -920
- data/doc/DuplicationValidationOutput.html +0 -564
- data/doc/FileNotFoundException.html +0 -134
- data/doc/GeneMergeValidation.html +0 -935
- data/doc/GeneMergeValidationOutput.html +0 -652
- data/doc/HierarchicalClusterization.html +0 -994
- data/doc/Hsp.html +0 -1485
- data/doc/InconsistentTabularFormat.html +0 -135
- data/doc/LengthClusterValidation.html +0 -982
- data/doc/LengthClusterValidationOutput.html +0 -515
- data/doc/LengthRankValidation.html +0 -496
- data/doc/LengthRankValidationOutput.html +0 -517
- data/doc/NoInternetError.html +0 -135
- data/doc/NoMafftInstallationError.html +0 -134
- data/doc/NoPIdentError.html +0 -134
- data/doc/NoValidationError.html +0 -134
- data/doc/NotEnoughHitsError.html +0 -135
- data/doc/ORFValidationOutput.html +0 -593
- data/doc/OpenReadingFrameValidation.html +0 -1107
- data/doc/OtherError.html +0 -123
- data/doc/Output.html +0 -1540
- data/doc/Pair.html +0 -309
- data/doc/PairCluster.html +0 -767
- data/doc/Plot.html +0 -837
- data/doc/QueryError.html +0 -134
- data/doc/ReportClassError.html +0 -135
- data/doc/Sequence.html +0 -1299
- data/doc/SequenceTypeError.html +0 -135
- data/doc/TabularEntry.html +0 -837
- data/doc/TabularParser.html +0 -1104
- data/doc/Validation.html +0 -2147
- data/doc/ValidationClassError.html +0 -134
- data/doc/ValidationOutput.html +0 -460
- data/doc/ValidationReport.html +0 -940
- data/doc/ValidationTest.html +0 -939
- data/doc/_index.html +0 -449
- data/doc/class_list.html +0 -54
- data/doc/css/common.css +0 -1
- data/doc/css/full_list.css +0 -57
- data/doc/css/style.css +0 -338
- data/doc/file.README.html +0 -151
- data/doc/file_list.html +0 -56
- data/doc/frames.html +0 -26
- data/doc/index.html +0 -151
- data/doc/js/app.js +0 -214
- data/doc/js/full_list.js +0 -178
- data/doc/js/jquery.js +0 -4
- data/doc/method_list.html +0 -1505
- data/doc/top-level-namespace.html +0 -112
- data/test/test_files/all_validations_mrna/all_validations_mrna.fasta.blast_tab +0 -967
- data/test/test_files/all_validations_mrna/all_validations_mrna.fasta.blast_tab.index +0 -967
- data/test/test_files/all_validations_mrna/all_validations_mrna.fasta.blast_tab.raw_seq +0 -4929
- data/test/test_files/all_validations_mrna/all_validations_mrna.fasta.blast_tab.raw_seq.idx +0 -1006
- data/test/test_files/all_validations_mrna/all_validations_mrna.fasta.blast_xml.raw_seq +0 -2075
- data/test/test_files/all_validations_prot/all_validations_prot.fasta.blast_tab.index +0 -1864
- data/test/test_files/all_validations_prot/all_validations_prot.fasta.blast_tab.raw_seq +0 -42411
- data/test/test_files/all_validations_prot/all_validations_prot.fasta.blast_tab.raw_seq.idx +0 -3751
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<a href="#initialize-instance_method" title="#initialize (instance method)">- (BlastReadingFrameValidation) <strong>initialize</strong>(type, prediction, hits = nil) </a>
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<pre class="code"><span class="info file"># File 'lib/genevalidator/validation_blast_reading_frame.rb', line 53</span>
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<span class='kw'>def</span> <span class='id identifier rubyid_initialize'>initialize</span><span class='lparen'>(</span><span class='id identifier rubyid_type'>type</span><span class='comma'>,</span> <span class='id identifier rubyid_prediction'>prediction</span><span class='comma'>,</span> <span class='id identifier rubyid_hits'>hits</span> <span class='op'>=</span> <span class='kw'>nil</span><span class='rparen'>)</span>
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<span class='ivar'>@short_header</span> <span class='op'>=</span> <span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>Frame</span><span class='tstring_end'>"</span></span>
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<span class='ivar'>@header</span> <span class='op'>=</span> <span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>Reading Frame</span><span class='tstring_end'>"</span></span>
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<span class='ivar'>@description</span> <span class='op'>=</span> <span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>Check whether there is a single reading frame among BLAST</span><span class='tstring_end'>"</span></span><span class='op'><<</span>
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<span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'> hits. Otherwise there might be a reading frame shift in the query sequence.</span><span class='tstring_end'>"</span></span><span class='op'><<</span>
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<pre class="code"><span class="info file"># File 'lib/genevalidator/validation_blast_reading_frame.rb', line 69</span>
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<span class='kw'>def</span> <span class='id identifier rubyid_run'>run</span><span class='lparen'>(</span><span class='id identifier rubyid_lst'>lst</span> <span class='op'>=</span> <span class='ivar'>@hits</span><span class='rparen'>)</span>
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<span class='kw'>begin</span>
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<span class='kw'>if</span> <span class='id identifier rubyid_type'>type</span><span class='period'>.</span><span class='id identifier rubyid_to_s'>to_s</span> <span class='op'>!=</span> <span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>nucleotide</span><span class='tstring_end'>"</span></span>
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<span class='ivar'>@validation_report</span> <span class='op'>=</span> <span class='const'>ValidationReport</span><span class='period'>.</span><span class='id identifier rubyid_new'>new</span><span class='lparen'>(</span><span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_end'>"</span></span><span class='comma'>,</span> <span class='symbol'>:unapplicable</span><span class='rparen'>)</span>
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<span class='kw'>return</span> <span class='ivar'>@validation_report</span>
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<span class='id identifier rubyid_raise'>raise</span> <span class='const'>NotEnoughHitsError</span> <span class='kw'>unless</span> <span class='id identifier rubyid_hits'>hits</span><span class='period'>.</span><span class='id identifier rubyid_length'>length</span> <span class='op'>>=</span> <span class='int'>5</span>
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<span class='id identifier rubyid_raise'>raise</span> <span class='const'>Exception</span> <span class='kw'>unless</span> <span class='id identifier rubyid_prediction'>prediction</span><span class='period'>.</span><span class='id identifier rubyid_is_a?'>is_a?</span> <span class='const'>Sequence</span> <span class='kw'>and</span> <span class='id identifier rubyid_hits'>hits</span><span class='lbracket'>[</span><span class='int'>0</span><span class='rbracket'>]</span><span class='period'>.</span><span class='id identifier rubyid_is_a?'>is_a?</span> <span class='const'>Sequence</span>
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<span class='id identifier rubyid_start'>start</span> <span class='op'>=</span> <span class='const'>Time</span><span class='period'>.</span><span class='id identifier rubyid_now'>now</span>
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<span class='id identifier rubyid_rfs'>rfs</span> <span class='op'>=</span> <span class='id identifier rubyid_lst'>lst</span><span class='period'>.</span><span class='id identifier rubyid_map'>map</span><span class='lbrace'>{</span> <span class='op'>|</span><span class='id identifier rubyid_x'>x</span><span class='op'>|</span> <span class='id identifier rubyid_x'>x</span><span class='period'>.</span><span class='id identifier rubyid_hsp_list'>hsp_list</span><span class='period'>.</span><span class='id identifier rubyid_map'>map</span><span class='lbrace'>{</span> <span class='op'>|</span><span class='id identifier rubyid_y'>y</span><span class='op'>|</span> <span class='id identifier rubyid_y'>y</span><span class='period'>.</span><span class='id identifier rubyid_query_reading_frame'>query_reading_frame</span><span class='rbrace'>}</span><span class='rbrace'>}</span><span class='period'>.</span><span class='id identifier rubyid_flatten'>flatten</span>
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<span class='id identifier rubyid_frames_histo'>frames_histo</span> <span class='op'>=</span> <span class='const'>Hash</span><span class='lbracket'>[</span><span class='id identifier rubyid_rfs'>rfs</span><span class='period'>.</span><span class='id identifier rubyid_group_by'>group_by</span> <span class='lbrace'>{</span> <span class='op'>|</span><span class='id identifier rubyid_x'>x</span><span class='op'>|</span> <span class='id identifier rubyid_x'>x</span> <span class='rbrace'>}</span><span class='period'>.</span><span class='id identifier rubyid_map'>map</span> <span class='lbrace'>{</span> <span class='op'>|</span><span class='id identifier rubyid_k'>k</span><span class='comma'>,</span> <span class='id identifier rubyid_vs'>vs</span><span class='op'>|</span> <span class='lbracket'>[</span><span class='id identifier rubyid_k'>k</span><span class='comma'>,</span> <span class='id identifier rubyid_vs'>vs</span><span class='period'>.</span><span class='id identifier rubyid_length'>length</span><span class='rbracket'>]</span> <span class='rbrace'>}</span><span class='rbracket'>]</span>
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<span class='comment'># get the main reading frame
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<span class='ivar'>@prediction</span><span class='period'>.</span><span class='id identifier rubyid_nucleotide_rf'>nucleotide_rf</span> <span class='op'>=</span> <span class='id identifier rubyid_frames_histo'>frames_histo</span><span class='period'>.</span><span class='id identifier rubyid_select'>select</span><span class='lbrace'>{</span><span class='op'>|</span><span class='id identifier rubyid_k'>k</span><span class='comma'>,</span><span class='id identifier rubyid_v'>v</span><span class='op'>|</span> <span class='id identifier rubyid_v'>v</span><span class='op'>==</span><span class='id identifier rubyid_main_rf'>main_rf</span><span class='rbrace'>}</span><span class='period'>.</span><span class='id identifier rubyid_first'>first</span><span class='period'>.</span><span class='id identifier rubyid_first'>first</span>
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<span class='ivar'>@validation_report</span> <span class='op'>=</span> <span class='const'>BlastRFValidationOutput</span><span class='period'>.</span><span class='id identifier rubyid_new'>new</span><span class='lparen'>(</span><span class='id identifier rubyid_frames_histo'>frames_histo</span><span class='rparen'>)</span>
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<span class='ivar'>@running_time</span> <span class='op'>=</span> <span class='const'>Time</span><span class='period'>.</span><span class='id identifier rubyid_now'>now</span> <span class='op'>-</span> <span class='id identifier rubyid_start'>start</span>
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<span class='ivar'>@validation_report</span> <span class='op'>=</span> <span class='const'>ValidationReport</span><span class='period'>.</span><span class='id identifier rubyid_new'>new</span><span class='lparen'>(</span><span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>Not enough evidence</span><span class='tstring_end'>"</span></span><span class='comma'>,</span> <span class='symbol'>:warning</span><span class='rparen'>)</span>
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<span class='ivar'>@validation_report</span> <span class='op'>=</span> <span class='const'>ValidationReport</span><span class='period'>.</span><span class='id identifier rubyid_new'>new</span><span class='lparen'>(</span><span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>Unexpected error</span><span class='tstring_end'>"</span></span><span class='comma'>,</span> <span class='symbol'>:error</span><span class='rparen'>)</span>
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<a href="#call_blast_from_file-class_method" title="call_blast_from_file (class method)">+ (Object) <strong>call_blast_from_file</strong>(command, filename, gapopen, gapextend, db = "nr -remote") </a>
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<a href="#call_blast_from_stdin-class_method" title="call_blast_from_stdin (class method)">+ (Object) <strong>call_blast_from_stdin</strong>(command, query, gapopen, gapextend, db = "nr -remote") </a>
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<p>Calls blast from standard input with specific parameters Params:
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<pre class="code"><span class="info file"># File 'lib/genevalidator/blast.rb', line 66</span>
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<span class='kw'>def</span> <span class='kw'>self</span><span class='period'>.</span><span class='id identifier rubyid_call_blast_from_file'>call_blast_from_file</span><span class='lparen'>(</span><span class='id identifier rubyid_command'>command</span><span class='comma'>,</span> <span class='id identifier rubyid_filename'>filename</span><span class='comma'>,</span> <span class='id identifier rubyid_gapopen'>gapopen</span><span class='comma'>,</span> <span class='id identifier rubyid_gapextend'>gapextend</span><span class='comma'>,</span> <span class='id identifier rubyid_db'>db</span><span class='op'>=</span><span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>nr -remote</span><span class='tstring_end'>"</span></span><span class='rparen'>)</span>
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<span class='kw'>begin</span>
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<span class='id identifier rubyid_evalue'>evalue</span> <span class='op'>=</span> <span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>1e-5</span><span class='tstring_end'>"</span></span>
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|
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</span> <span class='id identifier rubyid_cmd'>cmd</span> <span class='op'>=</span> <span class='tstring'><span class='tstring_beg'>"</span><span class='embexpr_beg'>#{</span><span class='id identifier rubyid_command'>command</span><span class='rbrace'>}</span><span class='tstring_content'> -query </span><span class='embexpr_beg'>#{</span><span class='id identifier rubyid_filename'>filename</span><span class='rbrace'>}</span><span class='tstring_content'> -db </span><span class='embexpr_beg'>#{</span><span class='id identifier rubyid_db'>db</span><span class='rbrace'>}</span><span class='tstring_content'> -evalue </span><span class='embexpr_beg'>#{</span><span class='id identifier rubyid_evalue'>evalue</span><span class='rbrace'>}</span><span class='tstring_content'> -outfmt 5 -gapopen </span><span class='embexpr_beg'>#{</span><span class='id identifier rubyid_gapopen'>gapopen</span><span class='rbrace'>}</span><span class='tstring_content'> -gapextend </span><span class='embexpr_beg'>#{</span><span class='id identifier rubyid_gapextend'>gapextend</span><span class='rbrace'>}</span><span class='tstring_content'> </span><span class='tstring_end'>"</span></span>
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<span class='id identifier rubyid_puts'>puts</span> <span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>Executing \"</span><span class='embexpr_beg'>#{</span><span class='id identifier rubyid_cmd'>cmd</span><span class='rbrace'>}</span><span class='tstring_content'>\"...</span><span class='tstring_end'>"</span></span>
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<span class='id identifier rubyid_puts'>puts</span> <span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>This may take a while...</span><span class='tstring_end'>"</span></span>
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<span class='id identifier rubyid_output'>output</span> <span class='op'>=</span> <span class='backtick'>%x[</span><span class='embexpr_beg'>#{</span><span class='id identifier rubyid_cmd'>cmd</span><span class='rbrace'>}</span><span class='tstring_content'> if xml_file == nil
|
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file = File.open(xml_file, "rb").read
|
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b.parse_xml_output(file)
|
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end 2>/dev/null</span><span class='tstring_end'>]</span></span>
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|
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|
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<span class='gvar'>$stderr</span><span class='period'>.</span><span class='id identifier rubyid_print'>print</span> <span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>Type error at </span><span class='embexpr_beg'>#{</span><span class='id identifier rubyid_error'>error</span><span class='period'>.</span><span class='id identifier rubyid_backtrace'>backtrace</span><span class='lbracket'>[</span><span class='int'>0</span><span class='rbracket'>]</span><span class='period'>.</span><span class='id identifier rubyid_scan'>scan</span><span class='lparen'>(</span><span class='tstring'><span class='regexp_beg'>/</span><span class='tstring_content'>\/([^\/]+:\d+):.*</span><span class='regexp_end'>/</span></span><span class='rparen'>)</span><span class='lbracket'>[</span><span class='int'>0</span><span class='rbracket'>]</span><span class='lbracket'>[</span><span class='int'>0</span><span class='rbracket'>]</span><span class='rbrace'>}</span><span class='tstring_content'>. </span><span class='tstring_end'>"</span></span><span class='op'><<</span>
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<span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>Possible cause: one of the arguments of 'call_blast_from_file' method has not the proper type\n</span><span class='tstring_end'>"</span></span>
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<span class='id identifier rubyid_exit'>exit</span>
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|
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<span class='gvar'>$stderr</span><span class='period'>.</span><span class='id identifier rubyid_print'>print</span> <span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>BLAST error at </span><span class='embexpr_beg'>#{</span><span class='id identifier rubyid_error'>error</span><span class='period'>.</span><span class='id identifier rubyid_backtrace'>backtrace</span><span class='lbracket'>[</span><span class='int'>0</span><span class='rbracket'>]</span><span class='period'>.</span><span class='id identifier rubyid_scan'>scan</span><span class='lparen'>(</span><span class='tstring'><span class='regexp_beg'>/</span><span class='tstring_content'>\/([^\/]+:\d+):.*</span><span class='regexp_end'>/</span></span><span class='rparen'>)</span><span class='lbracket'>[</span><span class='int'>0</span><span class='rbracket'>]</span><span class='lbracket'>[</span><span class='int'>0</span><span class='rbracket'>]</span><span class='rbrace'>}</span><span class='tstring_content'>. </span><span class='tstring_end'>"</span></span><span class='op'><<</span>
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<span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>Did you add BLAST path to LOADPATH?\n</span><span class='tstring_end'>"</span></span>
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|
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|
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|
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|
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<h3 class="signature " id="call_blast_from_stdin-class_method">
|
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+ (<tt>Object</tt>) <strong>call_blast_from_stdin</strong>(command, query, gapopen, gapextend, db = "nr -remote")
|
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</h3><div class="docstring">
|
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<div class="discussion">
|
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|
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|
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|
-
<p>Calls blast from standard input with specific parameters Params:
|
402
|
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<tt>command</tt>: blast command in String format (e.g 'blastx' or 'blastp')
|
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|
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<tt>query</tt>: String containing the the query in fasta format
|
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|
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<tt>gapopen</tt>: gapopen blast parameter <tt>gapextend</tt>: gapextend
|
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|
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blast parameter <tt>db</tt>: database Output: String with the blast xml
|
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output</p>
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<pre class="code"><span class="info file"># File 'lib/genevalidator/blast.rb', line 26</span>
|
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<span class='kw'>def</span> <span class='kw'>self</span><span class='period'>.</span><span class='id identifier rubyid_call_blast_from_stdin'>call_blast_from_stdin</span><span class='lparen'>(</span><span class='id identifier rubyid_command'>command</span><span class='comma'>,</span> <span class='id identifier rubyid_query'>query</span><span class='comma'>,</span> <span class='id identifier rubyid_gapopen'>gapopen</span><span class='comma'>,</span> <span class='id identifier rubyid_gapextend'>gapextend</span><span class='comma'>,</span> <span class='id identifier rubyid_db'>db</span><span class='op'>=</span><span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>nr -remote</span><span class='tstring_end'>"</span></span><span class='rparen'>)</span>
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<span class='kw'>begin</span>
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<span class='id identifier rubyid_raise'>raise</span> <span class='const'>TypeError</span> <span class='kw'>unless</span> <span class='id identifier rubyid_command'>command</span><span class='period'>.</span><span class='id identifier rubyid_is_a?'>is_a?</span> <span class='const'>String</span> <span class='kw'>and</span> <span class='id identifier rubyid_query'>query</span><span class='period'>.</span><span class='id identifier rubyid_is_a?'>is_a?</span> <span class='const'>String</span>
|
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<span class='id identifier rubyid_evalue'>evalue</span> <span class='op'>=</span> <span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>1e-5</span><span class='tstring_end'>"</span></span>
|
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|
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|
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|
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<span class='comment'>#output format = 5 (XML Blast output)
|
459
|
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</span> <span class='id identifier rubyid_blast_cmd'>blast_cmd</span> <span class='op'>=</span> <span class='tstring'><span class='tstring_beg'>"</span><span class='embexpr_beg'>#{</span><span class='id identifier rubyid_command'>command</span><span class='rbrace'>}</span><span class='tstring_content'> -db </span><span class='embexpr_beg'>#{</span><span class='id identifier rubyid_db'>db</span><span class='rbrace'>}</span><span class='tstring_content'> -evalue </span><span class='embexpr_beg'>#{</span><span class='id identifier rubyid_evalue'>evalue</span><span class='rbrace'>}</span><span class='tstring_content'> -outfmt 5 -gapopen </span><span class='embexpr_beg'>#{</span><span class='id identifier rubyid_gapopen'>gapopen</span><span class='rbrace'>}</span><span class='tstring_content'> -gapextend </span><span class='embexpr_beg'>#{</span><span class='id identifier rubyid_gapextend'>gapextend</span><span class='rbrace'>}</span><span class='tstring_end'>"</span></span>
|
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<span class='id identifier rubyid_cmd'>cmd</span> <span class='op'>=</span> <span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>echo \"</span><span class='embexpr_beg'>#{</span><span class='id identifier rubyid_query'>query</span><span class='rbrace'>}</span><span class='tstring_content'>\" | </span><span class='embexpr_beg'>#{</span><span class='id identifier rubyid_blast_cmd'>blast_cmd</span><span class='rbrace'>}</span><span class='tstring_end'>"</span></span>
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<span class='id identifier rubyid_output'>output</span> <span class='op'>=</span> <span class='backtick'>%x[</span><span class='embexpr_beg'>#{</span><span class='id identifier rubyid_cmd'>cmd</span><span class='rbrace'>}</span><span class='tstring_content'> 2>/dev/null</span><span class='tstring_end'>]</span></span>
|
462
|
-
|
463
|
-
<span class='kw'>if</span> <span class='id identifier rubyid_output'>output</span> <span class='op'>==</span> <span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_end'>"</span></span>
|
464
|
-
<span class='id identifier rubyid_raise'>raise</span> <span class='const'>ClasspathError</span><span class='period'>.</span><span class='id identifier rubyid_new'>new</span>
|
465
|
-
<span class='kw'>end</span>
|
466
|
-
|
467
|
-
<span class='kw'>return</span> <span class='id identifier rubyid_output'>output</span>
|
468
|
-
|
469
|
-
<span class='kw'>rescue</span> <span class='const'>TypeError</span> <span class='op'>=></span> <span class='id identifier rubyid_error'>error</span>
|
470
|
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<span class='gvar'>$stderr</span><span class='period'>.</span><span class='id identifier rubyid_print'>print</span> <span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>Type error at </span><span class='embexpr_beg'>#{</span><span class='id identifier rubyid_error'>error</span><span class='period'>.</span><span class='id identifier rubyid_backtrace'>backtrace</span><span class='lbracket'>[</span><span class='int'>0</span><span class='rbracket'>]</span><span class='period'>.</span><span class='id identifier rubyid_scan'>scan</span><span class='lparen'>(</span><span class='tstring'><span class='regexp_beg'>/</span><span class='tstring_content'>\/([^\/]+:\d+):.*</span><span class='regexp_end'>/</span></span><span class='rparen'>)</span><span class='lbracket'>[</span><span class='int'>0</span><span class='rbracket'>]</span><span class='lbracket'>[</span><span class='int'>0</span><span class='rbracket'>]</span><span class='rbrace'>}</span><span class='tstring_content'>. </span><span class='tstring_end'>"</span></span><span class='op'><<</span>
|
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|
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<span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>Possible cause: one of the arguments of 'call_blast_from_file' method has not the proper type\n</span><span class='tstring_end'>"</span></span>
|
472
|
-
<span class='id identifier rubyid_exit'>exit</span>
|
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|
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<span class='kw'>rescue</span> <span class='const'>ClasspathError</span> <span class='op'>=></span> <span class='id identifier rubyid_error'>error</span>
|
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|
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<span class='gvar'>$stderr</span><span class='period'>.</span><span class='id identifier rubyid_print'>print</span> <span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>BLAST error at </span><span class='embexpr_beg'>#{</span><span class='id identifier rubyid_error'>error</span><span class='period'>.</span><span class='id identifier rubyid_backtrace'>backtrace</span><span class='lbracket'>[</span><span class='int'>0</span><span class='rbracket'>]</span><span class='period'>.</span><span class='id identifier rubyid_scan'>scan</span><span class='lparen'>(</span><span class='tstring'><span class='regexp_beg'>/</span><span class='tstring_content'>\/([^\/]+:\d+):.*</span><span class='regexp_end'>/</span></span><span class='rparen'>)</span><span class='lbracket'>[</span><span class='int'>0</span><span class='rbracket'>]</span><span class='lbracket'>[</span><span class='int'>0</span><span class='rbracket'>]</span><span class='rbrace'>}</span><span class='tstring_content'>. </span><span class='tstring_end'>"</span></span><span class='op'><<</span>
|
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|
-
<span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>Possible cause: BLAST installation path is not in the LOAD PATH.\n</span><span class='tstring_end'>"</span></span>
|
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|
-
|
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|
-
<span class='id identifier rubyid_exit'>exit</span>
|
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|
-
<span class='kw'>end</span>
|
479
|
-
<span class='kw'>end</span></pre>
|
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|
-
</td>
|
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|
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|
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|
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</table>
|
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|
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</div>
|
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|
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|
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|
-
<div class="method_details ">
|
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|
-
<h3 class="signature " id="composition-class_method">
|
487
|
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|
488
|
-
+ (<tt>Object</tt>) <strong>composition</strong>(sequence_string)
|
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|
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|
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|
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491
|
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|
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|
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|
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|
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|
-
</h3><div class="docstring">
|
495
|
-
<div class="discussion">
|
496
|
-
|
497
|
-
<p>Method copied from sequenceserver/sequencehelpers.rb Params:
|
498
|
-
sequence_string: String of which we mfind the composition Output: a Hash</p>
|
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|
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|
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|
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|
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</div>
|
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|
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</div>
|
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|
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<div class="tags">
|
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|
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|
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|
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|
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|
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|
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|
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|
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<pre class="code"><span class="info file"># File 'lib/genevalidator/blast.rb', line 185</span>
|
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|
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|
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|
-
<span class='kw'>def</span> <span class='kw'>self</span><span class='period'>.</span><span class='id identifier rubyid_composition'>composition</span><span class='lparen'>(</span><span class='id identifier rubyid_sequence_string'>sequence_string</span><span class='rparen'>)</span>
|
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|
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<span class='id identifier rubyid_count'>count</span> <span class='op'>=</span> <span class='const'>Hash</span><span class='period'>.</span><span class='id identifier rubyid_new'>new</span><span class='lparen'>(</span><span class='int'>0</span><span class='rparen'>)</span>
|
525
|
-
<span class='id identifier rubyid_sequence_string'>sequence_string</span><span class='period'>.</span><span class='id identifier rubyid_scan'>scan</span><span class='lparen'>(</span><span class='tstring'><span class='regexp_beg'>/</span><span class='tstring_content'>.</span><span class='regexp_end'>/</span></span><span class='rparen'>)</span> <span class='kw'>do</span> <span class='op'>|</span><span class='id identifier rubyid_x'>x</span><span class='op'>|</span>
|
526
|
-
<span class='id identifier rubyid_count'>count</span><span class='lbracket'>[</span><span class='id identifier rubyid_x'>x</span><span class='rbracket'>]</span> <span class='op'>+=</span> <span class='int'>1</span>
|
527
|
-
<span class='kw'>end</span>
|
528
|
-
<span class='id identifier rubyid_count'>count</span>
|
529
|
-
<span class='kw'>end</span></pre>
|
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|
-
</td>
|
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|
-
</tr>
|
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|
-
</table>
|
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|
-
</div>
|
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|
-
|
535
|
-
<div class="method_details ">
|
536
|
-
<h3 class="signature " id="guess_sequence_type-class_method">
|
537
|
-
|
538
|
-
+ (<tt>Object</tt>) <strong>guess_sequence_type</strong>(sequence_string)
|
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|
-
|
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|
-
|
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|
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|
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|
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|
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|
-
|
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|
-
</h3><div class="docstring">
|
545
|
-
<div class="discussion">
|
546
|
-
|
547
|
-
<p>Method copied from sequenceserver/sequencehelpers.rb Strips all non-letter
|
548
|
-
characters. guestimates sequence based on that. If less than 10 useable
|
549
|
-
characters... returns nil If more than 90% ACGTU returns :nucleotide. else
|
550
|
-
returns :protein Params: <tt>sequence_string</tt>: String to validate
|
551
|
-
Output: nil, :nucleotide or :protein</p>
|
552
|
-
|
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|
-
|
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|
-
</div>
|
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|
-
</div>
|
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|
-
<div class="tags">
|
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|
-
|
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|
-
|
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|
-
</div><table class="source_code">
|
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|
-
<tr>
|
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<td>
|
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|
-
<pre class="lines">
|
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219</pre>
|
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|
-
</td>
|
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|
-
<td>
|
585
|
-
<pre class="code"><span class="info file"># File 'lib/genevalidator/blast.rb', line 202</span>
|
586
|
-
|
587
|
-
<span class='kw'>def</span> <span class='kw'>self</span><span class='period'>.</span><span class='id identifier rubyid_guess_sequence_type'>guess_sequence_type</span><span class='lparen'>(</span><span class='id identifier rubyid_sequence_string'>sequence_string</span><span class='rparen'>)</span>
|
588
|
-
<span class='id identifier rubyid_cleaned_sequence'>cleaned_sequence</span> <span class='op'>=</span> <span class='id identifier rubyid_sequence_string'>sequence_string</span><span class='period'>.</span><span class='id identifier rubyid_gsub'>gsub</span><span class='lparen'>(</span><span class='tstring'><span class='regexp_beg'>/</span><span class='tstring_content'>[^A-Z]</span><span class='regexp_end'>/i</span></span><span class='comma'>,</span> <span class='tstring'><span class='tstring_beg'>'</span><span class='tstring_end'>'</span></span><span class='rparen'>)</span> <span class='comment'># removing non-letter characters
|
589
|
-
</span> <span class='id identifier rubyid_cleaned_sequence'>cleaned_sequence</span><span class='period'>.</span><span class='id identifier rubyid_gsub!'>gsub!</span><span class='lparen'>(</span><span class='tstring'><span class='regexp_beg'>/</span><span class='tstring_content'>[NX]</span><span class='regexp_end'>/i</span></span><span class='comma'>,</span> <span class='tstring'><span class='tstring_beg'>'</span><span class='tstring_end'>'</span></span><span class='rparen'>)</span> <span class='comment'># removing ambiguous characters
|
590
|
-
</span>
|
591
|
-
<span class='kw'>return</span> <span class='kw'>nil</span> <span class='kw'>if</span> <span class='id identifier rubyid_cleaned_sequence'>cleaned_sequence</span><span class='period'>.</span><span class='id identifier rubyid_length'>length</span> <span class='op'><</span> <span class='int'>10</span> <span class='comment'># conservative
|
592
|
-
</span>
|
593
|
-
<span class='id identifier rubyid_composition'>composition</span> <span class='op'>=</span> <span class='const'>BlastUtils</span><span class='period'>.</span><span class='id identifier rubyid_composition'>composition</span><span class='lparen'>(</span><span class='id identifier rubyid_cleaned_sequence'>cleaned_sequence</span><span class='rparen'>)</span>
|
594
|
-
<span class='id identifier rubyid_composition_NAs'>composition_NAs</span> <span class='op'>=</span> <span class='id identifier rubyid_composition'>composition</span><span class='period'>.</span><span class='id identifier rubyid_select'>select</span> <span class='lbrace'>{</span> <span class='op'>|</span><span class='id identifier rubyid_character'>character</span><span class='comma'>,</span> <span class='id identifier rubyid_count'>count</span><span class='op'>|</span><span class='id identifier rubyid_character'>character</span><span class='period'>.</span><span class='id identifier rubyid_match'>match</span><span class='lparen'>(</span><span class='tstring'><span class='regexp_beg'>/</span><span class='tstring_content'>[ACGTU]</span><span class='regexp_end'>/i</span></span><span class='rparen'>)</span> <span class='rbrace'>}</span> <span class='comment'># only putative NAs
|
595
|
-
</span> <span class='id identifier rubyid_putative_NA_counts'>putative_NA_counts</span> <span class='op'>=</span> <span class='id identifier rubyid_composition_NAs'>composition_NAs</span><span class='period'>.</span><span class='id identifier rubyid_collect'>collect</span> <span class='lbrace'>{</span> <span class='op'>|</span><span class='id identifier rubyid_key_value_array'>key_value_array</span><span class='op'>|</span> <span class='id identifier rubyid_key_value_array'>key_value_array</span><span class='lbracket'>[</span><span class='int'>1</span><span class='rbracket'>]</span> <span class='rbrace'>}</span> <span class='comment'># only count, not char
|
596
|
-
</span> <span class='id identifier rubyid_putative_NA_sum'>putative_NA_sum</span> <span class='op'>=</span> <span class='id identifier rubyid_putative_NA_counts'>putative_NA_counts</span><span class='period'>.</span><span class='id identifier rubyid_inject'>inject</span> <span class='lbrace'>{</span> <span class='op'>|</span><span class='id identifier rubyid_sum'>sum</span><span class='comma'>,</span> <span class='id identifier rubyid_n'>n</span><span class='op'>|</span> <span class='id identifier rubyid_sum'>sum</span> <span class='op'>+</span> <span class='id identifier rubyid_n'>n</span> <span class='rbrace'>}</span> <span class='comment'># count of all putative NA
|
597
|
-
</span> <span class='id identifier rubyid_putative_NA_sum'>putative_NA_sum</span> <span class='op'>=</span> <span class='int'>0</span> <span class='kw'>if</span> <span class='id identifier rubyid_putative_NA_sum'>putative_NA_sum</span><span class='period'>.</span><span class='id identifier rubyid_nil?'>nil?</span>
|
598
|
-
|
599
|
-
<span class='kw'>if</span> <span class='id identifier rubyid_putative_NA_sum'>putative_NA_sum</span> <span class='op'>></span> <span class='lparen'>(</span><span class='float'>0.9</span> <span class='op'>*</span> <span class='id identifier rubyid_cleaned_sequence'>cleaned_sequence</span><span class='period'>.</span><span class='id identifier rubyid_length'>length</span><span class='rparen'>)</span>
|
600
|
-
<span class='kw'>return</span> <span class='symbol'>:nucleotide</span>
|
601
|
-
<span class='kw'>else</span>
|
602
|
-
<span class='kw'>return</span> <span class='symbol'>:protein</span>
|
603
|
-
<span class='kw'>end</span>
|
604
|
-
<span class='kw'>end</span></pre>
|
605
|
-
</td>
|
606
|
-
</tr>
|
607
|
-
</table>
|
608
|
-
</div>
|
609
|
-
|
610
|
-
<div class="method_details ">
|
611
|
-
<h3 class="signature " id="parse_next_query_xml-class_method">
|
612
|
-
|
613
|
-
+ (<tt>Object</tt>) <strong>parse_next_query_xml</strong>(iterator, type)
|
614
|
-
|
615
|
-
|
616
|
-
|
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|
-
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|
-
|
619
|
-
</h3><div class="docstring">
|
620
|
-
<div class="discussion">
|
621
|
-
|
622
|
-
<p>Parses the next query from the blast xml output query Params:
|
623
|
-
<tt>iterator</tt>: blast xml iterator for hits <tt>type</tt>: the type of
|
624
|
-
the sequence: :nucleotide or :protein Outputs: Array of <tt>Sequence</tt>
|
625
|
-
ojbects for hits</p>
|
626
|
-
|
627
|
-
|
628
|
-
</div>
|
629
|
-
</div>
|
630
|
-
<div class="tags">
|
631
|
-
|
632
|
-
|
633
|
-
</div><table class="source_code">
|
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|
-
<tr>
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<td>
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-
<pre class="lines">
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|
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177</pre>
|
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|
-
</td>
|
713
|
-
<td>
|
714
|
-
<pre class="code"><span class="info file"># File 'lib/genevalidator/blast.rb', line 105</span>
|
715
|
-
|
716
|
-
<span class='kw'>def</span> <span class='kw'>self</span><span class='period'>.</span><span class='id identifier rubyid_parse_next_query_xml'>parse_next_query_xml</span><span class='lparen'>(</span><span class='id identifier rubyid_iterator'>iterator</span><span class='comma'>,</span> <span class='id identifier rubyid_type'>type</span><span class='rparen'>)</span>
|
717
|
-
<span class='kw'>begin</span>
|
718
|
-
<span class='id identifier rubyid_raise'>raise</span> <span class='const'>TypeError</span> <span class='kw'>unless</span> <span class='id identifier rubyid_iterator'>iterator</span><span class='period'>.</span><span class='id identifier rubyid_is_a?'>is_a?</span> <span class='const'>Enumerator</span>
|
719
|
-
|
720
|
-
<span class='id identifier rubyid_hits'>hits</span> <span class='op'>=</span> <span class='const'>Array</span><span class='period'>.</span><span class='id identifier rubyid_new'>new</span>
|
721
|
-
<span class='id identifier rubyid_predicted_seq'>predicted_seq</span> <span class='op'>=</span> <span class='const'>Sequence</span><span class='period'>.</span><span class='id identifier rubyid_new'>new</span>
|
722
|
-
<span class='id identifier rubyid_iter'>iter</span> <span class='op'>=</span> <span class='id identifier rubyid_iterator'>iterator</span><span class='period'>.</span><span class='id identifier rubyid_next'>next</span>
|
723
|
-
|
724
|
-
<span class='comment'># parse blast the xml output and get the hits
|
725
|
-
</span> <span class='comment'># hits obtained are proteins! (we use only blastp and blastx)
|
726
|
-
</span> <span class='id identifier rubyid_iter'>iter</span><span class='period'>.</span><span class='id identifier rubyid_each'>each</span> <span class='kw'>do</span> <span class='op'>|</span> <span class='id identifier rubyid_hit'>hit</span> <span class='op'>|</span>
|
727
|
-
|
728
|
-
<span class='id identifier rubyid_seq'>seq</span> <span class='op'>=</span> <span class='const'>Sequence</span><span class='period'>.</span><span class='id identifier rubyid_new'>new</span>
|
729
|
-
|
730
|
-
<span class='id identifier rubyid_seq'>seq</span><span class='period'>.</span><span class='id identifier rubyid_length_protein'>length_protein</span> <span class='op'>=</span> <span class='id identifier rubyid_hit'>hit</span><span class='period'>.</span><span class='id identifier rubyid_len'>len</span><span class='period'>.</span><span class='id identifier rubyid_to_i'>to_i</span>
|
731
|
-
<span class='id identifier rubyid_seq'>seq</span><span class='period'>.</span><span class='id identifier rubyid_type'>type</span> <span class='op'>=</span> <span class='symbol'>:protein</span>
|
732
|
-
<span class='id identifier rubyid_seq'>seq</span><span class='period'>.</span><span class='id identifier rubyid_identifier'>identifier</span> <span class='op'>=</span> <span class='id identifier rubyid_hit'>hit</span><span class='period'>.</span><span class='id identifier rubyid_hit_id'>hit_id</span>
|
733
|
-
<span class='id identifier rubyid_seq'>seq</span><span class='period'>.</span><span class='id identifier rubyid_definition'>definition</span> <span class='op'>=</span> <span class='id identifier rubyid_hit'>hit</span><span class='period'>.</span><span class='id identifier rubyid_hit_def'>hit_def</span>
|
734
|
-
<span class='comment'>#puts seq.identifier
|
735
|
-
</span> <span class='id identifier rubyid_seq'>seq</span><span class='period'>.</span><span class='id identifier rubyid_accession_no'>accession_no</span> <span class='op'>=</span> <span class='id identifier rubyid_hit'>hit</span><span class='period'>.</span><span class='id identifier rubyid_accession'>accession</span>
|
736
|
-
|
737
|
-
<span class='comment'># get all high-scoring segment pairs (hsp)
|
738
|
-
</span> <span class='id identifier rubyid_hsps'>hsps</span> <span class='op'>=</span> <span class='lbracket'>[</span><span class='rbracket'>]</span>
|
739
|
-
<span class='id identifier rubyid_hit'>hit</span><span class='period'>.</span><span class='id identifier rubyid_hsps'>hsps</span><span class='period'>.</span><span class='id identifier rubyid_each'>each</span> <span class='kw'>do</span> <span class='op'>|</span><span class='id identifier rubyid_hsp'>hsp</span><span class='op'>|</span>
|
740
|
-
<span class='id identifier rubyid_current_hsp'>current_hsp</span> <span class='op'>=</span> <span class='const'>Hsp</span><span class='period'>.</span><span class='id identifier rubyid_new'>new</span>
|
741
|
-
<span class='id identifier rubyid_current_hsp'>current_hsp</span><span class='period'>.</span><span class='id identifier rubyid_hsp_evalue'>hsp_evalue</span> <span class='op'>=</span> <span class='id identifier rubyid_hsp'>hsp</span><span class='period'>.</span><span class='id identifier rubyid_evalue'>evalue</span><span class='period'>.</span><span class='id identifier rubyid_to_i'>to_i</span>
|
742
|
-
|
743
|
-
<span class='id identifier rubyid_current_hsp'>current_hsp</span><span class='period'>.</span><span class='id identifier rubyid_hit_from'>hit_from</span> <span class='op'>=</span> <span class='id identifier rubyid_hsp'>hsp</span><span class='period'>.</span><span class='id identifier rubyid_hit_from'>hit_from</span><span class='period'>.</span><span class='id identifier rubyid_to_i'>to_i</span>
|
744
|
-
<span class='id identifier rubyid_current_hsp'>current_hsp</span><span class='period'>.</span><span class='id identifier rubyid_hit_to'>hit_to</span> <span class='op'>=</span> <span class='id identifier rubyid_hsp'>hsp</span><span class='period'>.</span><span class='id identifier rubyid_hit_to'>hit_to</span><span class='period'>.</span><span class='id identifier rubyid_to_i'>to_i</span>
|
745
|
-
<span class='id identifier rubyid_current_hsp'>current_hsp</span><span class='period'>.</span><span class='id identifier rubyid_match_query_from'>match_query_from</span> <span class='op'>=</span> <span class='id identifier rubyid_hsp'>hsp</span><span class='period'>.</span><span class='id identifier rubyid_query_from'>query_from</span><span class='period'>.</span><span class='id identifier rubyid_to_i'>to_i</span>
|
746
|
-
<span class='id identifier rubyid_current_hsp'>current_hsp</span><span class='period'>.</span><span class='id identifier rubyid_match_query_to'>match_query_to</span> <span class='op'>=</span> <span class='id identifier rubyid_hsp'>hsp</span><span class='period'>.</span><span class='id identifier rubyid_query_to'>query_to</span><span class='period'>.</span><span class='id identifier rubyid_to_i'>to_i</span>
|
747
|
-
|
748
|
-
<span class='kw'>if</span> <span class='id identifier rubyid_type'>type</span> <span class='op'>==</span> <span class='symbol'>:nucleotide</span>
|
749
|
-
<span class='id identifier rubyid_current_hsp'>current_hsp</span><span class='period'>.</span><span class='id identifier rubyid_match_query_from'>match_query_from</span> <span class='op'>=</span> <span class='lparen'>(</span><span class='id identifier rubyid_current_hsp'>current_hsp</span><span class='period'>.</span><span class='id identifier rubyid_match_query_from'>match_query_from</span> <span class='op'>/</span> <span class='int'>3</span><span class='rparen'>)</span> <span class='op'>+</span> <span class='int'>1</span>
|
750
|
-
<span class='id identifier rubyid_current_hsp'>current_hsp</span><span class='period'>.</span><span class='id identifier rubyid_match_query_to'>match_query_to</span> <span class='op'>=</span> <span class='lparen'>(</span><span class='id identifier rubyid_current_hsp'>current_hsp</span><span class='period'>.</span><span class='id identifier rubyid_match_query_to'>match_query_to</span> <span class='op'>/</span> <span class='int'>3</span><span class='rparen'>)</span> <span class='op'>+</span> <span class='int'>1</span>
|
751
|
-
<span class='kw'>end</span>
|
752
|
-
|
753
|
-
<span class='id identifier rubyid_current_hsp'>current_hsp</span><span class='period'>.</span><span class='id identifier rubyid_query_reading_frame'>query_reading_frame</span> <span class='op'>=</span> <span class='id identifier rubyid_hsp'>hsp</span><span class='period'>.</span><span class='id identifier rubyid_query_frame'>query_frame</span><span class='period'>.</span><span class='id identifier rubyid_to_i'>to_i</span>
|
754
|
-
|
755
|
-
<span class='id identifier rubyid_current_hsp'>current_hsp</span><span class='period'>.</span><span class='id identifier rubyid_hit_alignment'>hit_alignment</span> <span class='op'>=</span> <span class='id identifier rubyid_hsp'>hsp</span><span class='period'>.</span><span class='id identifier rubyid_hseq'>hseq</span><span class='period'>.</span><span class='id identifier rubyid_to_s'>to_s</span>
|
756
|
-
<span class='kw'>if</span> <span class='const'>BlastUtils</span><span class='period'>.</span><span class='id identifier rubyid_guess_sequence_type'>guess_sequence_type</span><span class='lparen'>(</span><span class='id identifier rubyid_current_hsp'>current_hsp</span><span class='period'>.</span><span class='id identifier rubyid_hit_alignment'>hit_alignment</span><span class='rparen'>)</span> <span class='op'>!=</span> <span class='symbol'>:protein</span>
|
757
|
-
<span class='id identifier rubyid_raise'>raise</span> <span class='const'>SequenceTypeError</span>
|
758
|
-
<span class='kw'>end</span>
|
759
|
-
|
760
|
-
<span class='id identifier rubyid_current_hsp'>current_hsp</span><span class='period'>.</span><span class='id identifier rubyid_query_alignment'>query_alignment</span> <span class='op'>=</span> <span class='id identifier rubyid_hsp'>hsp</span><span class='period'>.</span><span class='id identifier rubyid_qseq'>qseq</span><span class='period'>.</span><span class='id identifier rubyid_to_s'>to_s</span>
|
761
|
-
<span class='kw'>if</span> <span class='const'>BlastUtils</span><span class='period'>.</span><span class='id identifier rubyid_guess_sequence_type'>guess_sequence_type</span><span class='lparen'>(</span><span class='id identifier rubyid_current_hsp'>current_hsp</span><span class='period'>.</span><span class='id identifier rubyid_query_alignment'>query_alignment</span><span class='rparen'>)</span> <span class='op'>!=</span> <span class='symbol'>:protein</span>
|
762
|
-
<span class='id identifier rubyid_raise'>raise</span> <span class='const'>SequenceTypeError</span>
|
763
|
-
<span class='kw'>end</span>
|
764
|
-
<span class='id identifier rubyid_current_hsp'>current_hsp</span><span class='period'>.</span><span class='id identifier rubyid_align_len'>align_len</span> <span class='op'>=</span> <span class='id identifier rubyid_hsp'>hsp</span><span class='period'>.</span><span class='id identifier rubyid_align_len'>align_len</span><span class='period'>.</span><span class='id identifier rubyid_to_i'>to_i</span>
|
765
|
-
<span class='id identifier rubyid_current_hsp'>current_hsp</span><span class='period'>.</span><span class='id identifier rubyid_identity'>identity</span> <span class='op'>=</span> <span class='id identifier rubyid_hsp'>hsp</span><span class='period'>.</span><span class='id identifier rubyid_identity'>identity</span><span class='period'>.</span><span class='id identifier rubyid_to_i'>to_i</span>
|
766
|
-
<span class='id identifier rubyid_current_hsp'>current_hsp</span><span class='period'>.</span><span class='id identifier rubyid_pidentity'>pidentity</span> <span class='op'>=</span> <span class='int'>100</span> <span class='op'>*</span> <span class='id identifier rubyid_hsp'>hsp</span><span class='period'>.</span><span class='id identifier rubyid_identity'>identity</span> <span class='op'>/</span> <span class='lparen'>(</span><span class='id identifier rubyid_hsp'>hsp</span><span class='period'>.</span><span class='id identifier rubyid_align_len'>align_len</span> <span class='op'>+</span> <span class='float'>0.0</span><span class='rparen'>)</span>
|
767
|
-
|
768
|
-
<span class='id identifier rubyid_hsps'>hsps</span><span class='period'>.</span><span class='id identifier rubyid_push'>push</span><span class='lparen'>(</span><span class='id identifier rubyid_current_hsp'>current_hsp</span><span class='rparen'>)</span>
|
769
|
-
<span class='kw'>end</span>
|
770
|
-
|
771
|
-
<span class='id identifier rubyid_seq'>seq</span><span class='period'>.</span><span class='id identifier rubyid_hsp_list'>hsp_list</span> <span class='op'>=</span> <span class='id identifier rubyid_hsps'>hsps</span>
|
772
|
-
<span class='id identifier rubyid_hits'>hits</span><span class='period'>.</span><span class='id identifier rubyid_push'>push</span><span class='lparen'>(</span><span class='id identifier rubyid_seq'>seq</span><span class='rparen'>)</span>
|
773
|
-
<span class='kw'>end</span>
|
774
|
-
|
775
|
-
<span class='kw'>return</span> <span class='id identifier rubyid_hits'>hits</span>
|
776
|
-
|
777
|
-
<span class='kw'>rescue</span> <span class='const'>TypeError</span> <span class='op'>=></span> <span class='id identifier rubyid_error'>error</span>
|
778
|
-
<span class='gvar'>$stderr</span><span class='period'>.</span><span class='id identifier rubyid_print'>print</span> <span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>Type error at </span><span class='embexpr_beg'>#{</span><span class='id identifier rubyid_error'>error</span><span class='period'>.</span><span class='id identifier rubyid_backtrace'>backtrace</span><span class='lbracket'>[</span><span class='int'>0</span><span class='rbracket'>]</span><span class='period'>.</span><span class='id identifier rubyid_scan'>scan</span><span class='lparen'>(</span><span class='tstring'><span class='regexp_beg'>/</span><span class='tstring_content'>\/([^\/]+:\d+):.*</span><span class='regexp_end'>/</span></span><span class='rparen'>)</span><span class='lbracket'>[</span><span class='int'>0</span><span class='rbracket'>]</span><span class='lbracket'>[</span><span class='int'>0</span><span class='rbracket'>]</span><span class='rbrace'>}</span><span class='tstring_content'>. </span><span class='tstring_end'>"</span></span><span class='op'><<</span>
|
779
|
-
<span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>Possible cause: you didn't call parse method first!\n</span><span class='tstring_end'>"</span></span>
|
780
|
-
<span class='id identifier rubyid_exit!'>exit!</span>
|
781
|
-
<span class='kw'>rescue</span> <span class='const'>SequenceTypeError</span> <span class='op'>=></span> <span class='id identifier rubyid_error'>error</span>
|
782
|
-
<span class='gvar'>$stderr</span><span class='period'>.</span><span class='id identifier rubyid_print'>print</span> <span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>Sequence Type error at </span><span class='embexpr_beg'>#{</span><span class='id identifier rubyid_error'>error</span><span class='period'>.</span><span class='id identifier rubyid_backtrace'>backtrace</span><span class='lbracket'>[</span><span class='int'>0</span><span class='rbracket'>]</span><span class='period'>.</span><span class='id identifier rubyid_scan'>scan</span><span class='lparen'>(</span><span class='tstring'><span class='regexp_beg'>/</span><span class='tstring_content'>\/([^\/]+:\d+):.*</span><span class='regexp_end'>/</span></span><span class='rparen'>)</span><span class='lbracket'>[</span><span class='int'>0</span><span class='rbracket'>]</span><span class='lbracket'>[</span><span class='int'>0</span><span class='rbracket'>]</span><span class='rbrace'>}</span><span class='tstring_content'>. </span><span class='tstring_end'>"</span></span><span class='op'><<</span>
|
783
|
-
<span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>Possible cause: the blast output was not obtained against a protein database.\n</span><span class='tstring_end'>"</span></span>
|
784
|
-
<span class='id identifier rubyid_exit!'>exit!</span>
|
785
|
-
<span class='kw'>rescue</span> <span class='const'>StopIteration</span>
|
786
|
-
<span class='kw'>nil</span>
|
787
|
-
<span class='kw'>end</span>
|
788
|
-
<span class='kw'>end</span></pre>
|
789
|
-
</td>
|
790
|
-
</tr>
|
791
|
-
</table>
|
792
|
-
</div>
|
793
|
-
|
794
|
-
<div class="method_details ">
|
795
|
-
<h3 class="signature " id="type_of_sequences-class_method">
|
796
|
-
|
797
|
-
+ (<tt>Object</tt>) <strong>type_of_sequences</strong>(fasta_format_string)
|
798
|
-
|
799
|
-
|
800
|
-
|
801
|
-
|
802
|
-
|
803
|
-
</h3><div class="docstring">
|
804
|
-
<div class="discussion">
|
805
|
-
|
806
|
-
<p>Method copied from sequenceserver/sequencehelpers.rb Splits input at
|
807
|
-
putative fasta definition lines (like ">adsfadsf"), guesses sequence
|
808
|
-
type for each sequence. If not enough sequence to determine, returns nil.
|
809
|
-
If 2 kinds of sequence mixed together, raises ArgumentError Otherwise,
|
810
|
-
returns :nucleotide or :protein Params: <tt>sequence_string</tt>: String to
|
811
|
-
validate Output: nil, :nucleotide or :protein</p>
|
812
|
-
|
813
|
-
|
814
|
-
</div>
|
815
|
-
</div>
|
816
|
-
<div class="tags">
|
817
|
-
|
818
|
-
|
819
|
-
</div><table class="source_code">
|
820
|
-
<tr>
|
821
|
-
<td>
|
822
|
-
<pre class="lines">
|
823
|
-
|
824
|
-
|
825
|
-
231
|
826
|
-
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|
827
|
-
233
|
828
|
-
234
|
829
|
-
235
|
830
|
-
236
|
831
|
-
237
|
832
|
-
238
|
833
|
-
239
|
834
|
-
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|
835
|
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836
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-
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|
837
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-
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|
838
|
-
244
|
839
|
-
245</pre>
|
840
|
-
</td>
|
841
|
-
<td>
|
842
|
-
<pre class="code"><span class="info file"># File 'lib/genevalidator/blast.rb', line 231</span>
|
843
|
-
|
844
|
-
<span class='kw'>def</span> <span class='kw'>self</span><span class='period'>.</span><span class='id identifier rubyid_type_of_sequences'>type_of_sequences</span><span class='lparen'>(</span><span class='id identifier rubyid_fasta_format_string'>fasta_format_string</span><span class='rparen'>)</span>
|
845
|
-
<span class='comment'># the first sequence does not need to have a fasta definition line
|
846
|
-
</span> <span class='id identifier rubyid_sequences'>sequences</span> <span class='op'>=</span> <span class='id identifier rubyid_fasta_format_string'>fasta_format_string</span><span class='period'>.</span><span class='id identifier rubyid_split'>split</span><span class='lparen'>(</span><span class='tstring'><span class='regexp_beg'>/</span><span class='tstring_content'>^>.*$</span><span class='regexp_end'>/</span></span><span class='rparen'>)</span><span class='period'>.</span><span class='id identifier rubyid_delete_if'>delete_if</span> <span class='lbrace'>{</span> <span class='op'>|</span><span class='id identifier rubyid_seq'>seq</span><span class='op'>|</span> <span class='id identifier rubyid_seq'>seq</span><span class='period'>.</span><span class='id identifier rubyid_empty?'>empty?</span> <span class='rbrace'>}</span>
|
847
|
-
|
848
|
-
<span class='comment'># get all sequence types
|
849
|
-
</span> <span class='id identifier rubyid_sequence_types'>sequence_types</span> <span class='op'>=</span> <span class='id identifier rubyid_sequences'>sequences</span><span class='period'>.</span><span class='id identifier rubyid_collect'>collect</span> <span class='lbrace'>{</span> <span class='op'>|</span><span class='id identifier rubyid_seq'>seq</span><span class='op'>|</span> <span class='const'>BlastUtils</span><span class='period'>.</span><span class='id identifier rubyid_guess_sequence_type'>guess_sequence_type</span><span class='lparen'>(</span><span class='id identifier rubyid_seq'>seq</span><span class='rparen'>)</span> <span class='rbrace'>}</span><span class='period'>.</span><span class='id identifier rubyid_uniq'>uniq</span><span class='period'>.</span><span class='id identifier rubyid_compact'>compact</span>
|
850
|
-
|
851
|
-
<span class='kw'>return</span> <span class='kw'>nil</span> <span class='kw'>if</span> <span class='id identifier rubyid_sequence_types'>sequence_types</span><span class='period'>.</span><span class='id identifier rubyid_empty?'>empty?</span>
|
852
|
-
|
853
|
-
<span class='kw'>if</span> <span class='id identifier rubyid_sequence_types'>sequence_types</span><span class='period'>.</span><span class='id identifier rubyid_length'>length</span> <span class='op'>==</span> <span class='int'>1</span>
|
854
|
-
<span class='kw'>return</span> <span class='id identifier rubyid_sequence_types'>sequence_types</span><span class='period'>.</span><span class='id identifier rubyid_first'>first</span> <span class='comment'># there is only one (but yes its an array)
|
855
|
-
</span> <span class='kw'>else</span>
|
856
|
-
<span class='id identifier rubyid_raise'>raise</span> <span class='const'>SequenceTypeError</span>
|
857
|
-
<span class='kw'>end</span>
|
858
|
-
<span class='kw'>end</span></pre>
|
859
|
-
</td>
|
860
|
-
</tr>
|
861
|
-
</table>
|
862
|
-
</div>
|
863
|
-
|
864
|
-
</div>
|
865
|
-
|
866
|
-
</div>
|
867
|
-
|
868
|
-
<div id="footer">
|
869
|
-
Generated on Sat Sep 28 07:01:32 2013 by
|
870
|
-
<a href="http://yardoc.org" title="Yay! A Ruby Documentation Tool" target="_parent">yard</a>
|
871
|
-
0.8.7.2 (ruby-1.9.3).
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872
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874
|
-
</body>
|
875
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