genevalidator 1.6.1 → 1.6.2
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- checksums.yaml +4 -4
- data/.gitignore +3 -1
- data/.travis.yml +2 -0
- data/README.md +78 -30
- data/Rakefile +11 -8
- data/aux/app_template_footer.erb +1 -6
- data/aux/app_template_header.erb +12 -32
- data/aux/files/css/style.css +2 -8
- data/aux/files/js/plots.js +564 -576
- data/aux/files/js/script.js +10 -0
- data/aux/json_footer.erb +8 -0
- data/aux/json_header.erb +19 -0
- data/aux/json_query.erb +14 -0
- data/aux/template_footer.erb +9 -58
- data/aux/template_header.erb +18 -58
- data/aux/template_query.erb +8 -36
- data/bin/genevalidator +45 -32
- data/genevalidator.gemspec +11 -7
- data/lib/genevalidator.rb +75 -455
- data/lib/genevalidator/arg_validation.rb +78 -107
- data/lib/genevalidator/blast.rb +57 -60
- data/lib/genevalidator/clusterization.rb +15 -15
- data/lib/genevalidator/exceptions.rb +32 -5
- data/lib/genevalidator/get_raw_sequences.rb +70 -33
- data/lib/genevalidator/hsp.rb +1 -4
- data/lib/genevalidator/json_to_gv_results.rb +109 -0
- data/lib/genevalidator/output.rb +177 -185
- data/lib/genevalidator/pool.rb +2 -1
- data/lib/genevalidator/sequences.rb +3 -3
- data/lib/genevalidator/tabular_parser.rb +24 -18
- data/lib/genevalidator/validation.rb +279 -0
- data/lib/genevalidator/validation_alignment.rb +31 -47
- data/lib/genevalidator/validation_blast_reading_frame.rb +19 -18
- data/lib/genevalidator/validation_duplication.rb +23 -19
- data/lib/genevalidator/validation_gene_merge.rb +30 -65
- data/lib/genevalidator/validation_length_cluster.rb +14 -53
- data/lib/genevalidator/validation_length_rank.rb +10 -11
- data/lib/genevalidator/validation_open_reading_frame.rb +18 -19
- data/lib/genevalidator/validation_report.rb +2 -5
- data/lib/genevalidator/validation_test.rb +8 -4
- data/lib/genevalidator/version.rb +1 -1
- data/test/test_all_validations.rb +51 -66
- data/test/test_blast.rb +68 -51
- data/test/test_clusterization.rb +1 -1
- data/test/test_clusterization_2d.rb +19 -13
- data/test/test_extended_array_methods.rb +1 -1
- data/test/test_files/all_validations_mrna/mrna.blast_tab6 +1806 -0
- data/test/test_files/all_validations_mrna/mrna.blast_tab7 +1865 -0
- data/test/test_files/all_validations_mrna/{all_validations_mrna.fasta.blast_xml → mrna.blast_xml} +18642 -1
- data/test/test_files/all_validations_mrna/{all_validations_mrna.fasta.blast_xml.index → mrna.blast_xml.index} +300 -0
- data/test/test_files/all_validations_mrna/{all_validations_mrna.fasta → mrna.fa} +0 -0
- data/test/test_files/all_validations_mrna/mrna.raw_seq +3970 -0
- data/test/test_files/all_validations_mrna/{all_validations_mrna.fasta.blast_xml.raw_seq.idx → mrna.raw_seq.idx} +901 -1
- data/test/test_files/all_validations_prot/{all_validations_prot.fasta.blast_tab → prot.blast_tab6} +416 -0
- data/test/test_files/all_validations_prot/prot.blast_tab7 +2400 -0
- data/test/test_files/all_validations_prot/{all_validations_prot.fasta.blast_xml → prot.blast_xml} +18299 -6723
- data/test/test_files/all_validations_prot/{all_validations_prot.fasta.blast_xml.index → prot.blast_xml.index} +408 -0
- data/test/test_files/all_validations_prot/{all_validations_prot.fasta → prot.fa} +0 -0
- data/test/test_files/all_validations_prot/{all_validations_prot.fasta.blast_xml.raw_seq → prot.raw_seq} +2735 -0
- data/test/test_files/all_validations_prot/{all_validations_prot.fasta.blast_xml.raw_seq.idx → prot.raw_seq.idx} +3032 -1808
- data/test/test_sequences.rb +46 -41
- data/test/test_validation_open_reading_frame.rb +318 -202
- data/test/test_validations.rb +48 -32
- metadata +76 -102
- data/doc/AliasDuplicationError.html +0 -134
- data/doc/AlignmentValidation.html +0 -1687
- data/doc/AlignmentValidationOutput.html +0 -659
- data/doc/Blast.html +0 -1905
- data/doc/BlastRFValidationOutput.html +0 -545
- data/doc/BlastReadingFrameValidation.html +0 -370
- data/doc/BlastUtils.html +0 -875
- data/doc/ClasspathError.html +0 -134
- data/doc/Cluster.html +0 -1316
- data/doc/DuplciationValidationOutput.html +0 -564
- data/doc/DuplicationValidation.html +0 -920
- data/doc/DuplicationValidationOutput.html +0 -564
- data/doc/FileNotFoundException.html +0 -134
- data/doc/GeneMergeValidation.html +0 -935
- data/doc/GeneMergeValidationOutput.html +0 -652
- data/doc/HierarchicalClusterization.html +0 -994
- data/doc/Hsp.html +0 -1485
- data/doc/InconsistentTabularFormat.html +0 -135
- data/doc/LengthClusterValidation.html +0 -982
- data/doc/LengthClusterValidationOutput.html +0 -515
- data/doc/LengthRankValidation.html +0 -496
- data/doc/LengthRankValidationOutput.html +0 -517
- data/doc/NoInternetError.html +0 -135
- data/doc/NoMafftInstallationError.html +0 -134
- data/doc/NoPIdentError.html +0 -134
- data/doc/NoValidationError.html +0 -134
- data/doc/NotEnoughHitsError.html +0 -135
- data/doc/ORFValidationOutput.html +0 -593
- data/doc/OpenReadingFrameValidation.html +0 -1107
- data/doc/OtherError.html +0 -123
- data/doc/Output.html +0 -1540
- data/doc/Pair.html +0 -309
- data/doc/PairCluster.html +0 -767
- data/doc/Plot.html +0 -837
- data/doc/QueryError.html +0 -134
- data/doc/ReportClassError.html +0 -135
- data/doc/Sequence.html +0 -1299
- data/doc/SequenceTypeError.html +0 -135
- data/doc/TabularEntry.html +0 -837
- data/doc/TabularParser.html +0 -1104
- data/doc/Validation.html +0 -2147
- data/doc/ValidationClassError.html +0 -134
- data/doc/ValidationOutput.html +0 -460
- data/doc/ValidationReport.html +0 -940
- data/doc/ValidationTest.html +0 -939
- data/doc/_index.html +0 -449
- data/doc/class_list.html +0 -54
- data/doc/css/common.css +0 -1
- data/doc/css/full_list.css +0 -57
- data/doc/css/style.css +0 -338
- data/doc/file.README.html +0 -151
- data/doc/file_list.html +0 -56
- data/doc/frames.html +0 -26
- data/doc/index.html +0 -151
- data/doc/js/app.js +0 -214
- data/doc/js/full_list.js +0 -178
- data/doc/js/jquery.js +0 -4
- data/doc/method_list.html +0 -1505
- data/doc/top-level-namespace.html +0 -112
- data/test/test_files/all_validations_mrna/all_validations_mrna.fasta.blast_tab +0 -967
- data/test/test_files/all_validations_mrna/all_validations_mrna.fasta.blast_tab.index +0 -967
- data/test/test_files/all_validations_mrna/all_validations_mrna.fasta.blast_tab.raw_seq +0 -4929
- data/test/test_files/all_validations_mrna/all_validations_mrna.fasta.blast_tab.raw_seq.idx +0 -1006
- data/test/test_files/all_validations_mrna/all_validations_mrna.fasta.blast_xml.raw_seq +0 -2075
- data/test/test_files/all_validations_prot/all_validations_prot.fasta.blast_tab.index +0 -1864
- data/test/test_files/all_validations_prot/all_validations_prot.fasta.blast_tab.raw_seq +0 -42411
- data/test/test_files/all_validations_prot/all_validations_prot.fasta.blast_tab.raw_seq.idx +0 -3751
data/test/test_validations.rb
CHANGED
@@ -11,13 +11,30 @@ require 'genevalidator/validation_gene_merge'
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|
11
11
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require 'genevalidator/validation_duplication'
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12
12
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require 'genevalidator/validation_open_reading_frame'
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13
13
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require 'genevalidator/validation_alignment'
|
14
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+
require 'genevalidator/validation'
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14
15
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15
16
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module GeneValidator
|
17
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+
class Validate
|
18
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+
# Extend Validate Class with an alternative validate method that
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19
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+
# doesn't produce the output and returns the output instance
|
20
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+
def validate_without_output(prediction, hits, current_idx)
|
21
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+
hits = remove_identical_hits(prediction, hits)
|
22
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+
vals = create_validation_tests(prediction, hits)
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23
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+
check_validations(vals)
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24
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+
vals.each(&:run)
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25
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+
@run_output = Output.new(current_idx, hits.length, prediction.definition)
|
26
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+
@run_output.validations = vals.map(&:validation_report)
|
27
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+
check_validations_output(vals)
|
28
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+
@run_output
|
29
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+
end
|
30
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+
end
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31
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+
|
32
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+
# Test the output produced by the validations
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16
33
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class ValidateOutput < Minitest::Test
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17
34
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filename_fasta = 'test/test_files/test_sequences.fasta'
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18
35
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filename_xml = "#{filename_fasta}.blast_xml"
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19
36
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filename_xml_raw = "#{filename_fasta}.blast_xml.raw_seq"
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20
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-
FileUtils.rm_rf("#{filename_fasta}.html") rescue
|
37
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+
FileUtils.rm_rf("#{filename_fasta}.html") rescue Errno::ENOENT
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21
38
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opt = {
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22
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input_fasta_file: filename_fasta,
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23
40
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validations: ['all'],
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@@ -26,11 +43,10 @@ module GeneValidator
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26
43
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num_threads: 1,
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27
44
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test: true
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28
45
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}
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29
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-
|
30
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-
val = GeneValidator::
|
46
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+
GeneValidator.init(opt)
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47
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+
val = GeneValidator::Validate.new
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31
48
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xml = File.open(filename_xml, 'rb').read
|
32
49
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iterator = Bio::BlastXMLParser::NokogiriBlastXml.new(xml).to_enum
|
33
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-
val.create_an_index_file_of_raw_seq_file(opt[:raw_sequences])
|
34
50
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|
35
51
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describe 'Detailed Validation of normal Insulin Query' do
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36
52
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hits = BlastUtils.parse_next(iterator, :nucleotide)
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@@ -48,7 +64,7 @@ module GeneValidator
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48
64
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'AAACTACTGCAACTAG'
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49
65
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50
66
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prediction.length_protein = 108
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51
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-
vals = val.
|
67
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+
vals = val.validate_without_output(prediction, hits, 1).validations
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52
68
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|
53
69
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lcv = vals.select { |v| v.class == LengthClusterValidationOutput }[0]
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54
70
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lrv = vals.select { |v| v.class == LengthRankValidationOutput }[0]
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@@ -96,7 +112,7 @@ module GeneValidator
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96
112
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end
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97
113
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|
98
114
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it 'should validate blast reading frame' do
|
99
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-
assert_equal({ 1 => 500 }, rfv.
|
115
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+
assert_equal({ 1 => 500 }, rfv.frames)
|
100
116
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assert_equal(500, rfv.total_hsp)
|
101
117
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assert_equal(:yes, rfv.result)
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102
118
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end
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@@ -147,7 +163,7 @@ module GeneValidator
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147
163
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'GAGCGGGG'
|
148
164
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prediction.length_protein = 46
|
149
165
|
|
150
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-
vals = val.
|
166
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+
vals = val.validate_without_output(prediction, hits, 1).validations
|
151
167
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|
152
168
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lcv = vals.select { |v| v.class == LengthClusterValidationOutput }[0]
|
153
169
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lrv = vals.select { |v| v.class == LengthRankValidationOutput }[0]
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@@ -190,15 +206,15 @@ module GeneValidator
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190
206
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'CAACTGGAAAACTACTGCAACTAG'
|
191
207
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prediction.length_protein = 46
|
192
208
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|
193
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-
|
209
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+
vals = val.validate_without_output(prediction, hits, 1).validations
|
194
210
|
|
195
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-
lcv =
|
196
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-
lrv =
|
197
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-
rfv =
|
198
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-
gmv =
|
199
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-
dv =
|
200
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-
av =
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201
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-
ov =
|
211
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+
lcv = vals.select { |v| v.class == LengthClusterValidationOutput }[0]
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212
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+
lrv = vals.select { |v| v.class == LengthRankValidationOutput }[0]
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213
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+
rfv = vals.select { |v| v.class == BlastRFValidationOutput }[0]
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214
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+
gmv = vals.select { |v| v.class == GeneMergeValidationOutput }[0]
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215
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+
dv = vals.select { |v| v.class == DuplicationValidationOutput }[0]
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216
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+
av = vals.select { |v| v.class == AlignmentValidationOutput }[0]
|
217
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+
ov = vals.select { |v| v.class == ORFValidationOutput }[0]
|
202
218
|
|
203
219
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it 'should validate as expected' do
|
204
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assert_equal(:no, lcv.result)
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@@ -232,15 +248,15 @@ module GeneValidator
|
|
232
248
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'AAGGCCGCTGCTTCGGGCCCAGCATCTGCTGCGCGGACGAGC'
|
233
249
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prediction.length_protein = 175
|
234
250
|
|
235
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-
|
251
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+
vals = val.validate_without_output(prediction, hits, 1).validations
|
236
252
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|
237
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-
lcv =
|
238
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-
lrv =
|
239
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-
rfv =
|
240
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-
gmv =
|
241
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-
dv =
|
242
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-
av =
|
243
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-
ov =
|
253
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+
lcv = vals.select { |v| v.class == LengthClusterValidationOutput }[0]
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254
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+
lrv = vals.select { |v| v.class == LengthRankValidationOutput }[0]
|
255
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+
rfv = vals.select { |v| v.class == BlastRFValidationOutput }[0]
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256
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+
gmv = vals.select { |v| v.class == GeneMergeValidationOutput }[0]
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257
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+
dv = vals.select { |v| v.class == DuplicationValidationOutput }[0]
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258
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+
av = vals.select { |v| v.class == AlignmentValidationOutput }[0]
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+
ov = vals.select { |v| v.class == ORFValidationOutput }[0]
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244
260
|
|
245
261
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it 'should validate length cluster validation' do
|
246
262
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assert_equal(:no, lcv.result)
|
@@ -320,15 +336,15 @@ module GeneValidator
|
|
320
336
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'AGATATTGTTAC'
|
321
337
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prediction.length_protein = 840
|
322
338
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|
323
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-
|
339
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+
vals = val.validate_without_output(prediction, hits, 1).validations
|
324
340
|
|
325
|
-
lcv =
|
326
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-
lrv =
|
327
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-
rfv =
|
328
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-
gmv =
|
329
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-
dv =
|
330
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-
av =
|
331
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-
ov =
|
341
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+
lcv = vals.select { |v| v.class == LengthClusterValidationOutput }[0]
|
342
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+
lrv = vals.select { |v| v.class == LengthRankValidationOutput }[0]
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343
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+
rfv = vals.select { |v| v.class == BlastRFValidationOutput }[0]
|
344
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+
gmv = vals.select { |v| v.class == GeneMergeValidationOutput }[0]
|
345
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+
dv = vals.select { |v| v.class == DuplicationValidationOutput }[0]
|
346
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+
av = vals.select { |v| v.class == AlignmentValidationOutput }[0]
|
347
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+
ov = vals.select { |v| v.class == ORFValidationOutput }[0]
|
332
348
|
|
333
349
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it 'should validate length cluster validation' do
|
334
350
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assert_equal(:no, lcv.result)
|
@@ -340,6 +356,6 @@ module GeneValidator
|
|
340
356
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assert_equal(:no, av.result)
|
341
357
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end
|
342
358
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end
|
343
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-
FileUtils.rm_rf("#{filename_fasta}.html") rescue
|
359
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+
FileUtils.rm_rf("#{filename_fasta}.html") rescue Errno::ENOENT
|
344
360
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end
|
345
361
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end
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metadata
CHANGED
@@ -1,7 +1,7 @@
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1
1
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--- !ruby/object:Gem::Specification
|
2
2
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name: genevalidator
|
3
3
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version: !ruby/object:Gem::Version
|
4
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-
version: 1.6.
|
4
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+
version: 1.6.2
|
5
5
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platform: ruby
|
6
6
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authors:
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7
7
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- Monica Dragan
|
@@ -11,7 +11,7 @@ authors:
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11
11
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autorequire:
|
12
12
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bindir: bin
|
13
13
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cert_chain: []
|
14
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-
date: 2015-
|
14
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+
date: 2015-07-18 00:00:00.000000000 Z
|
15
15
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dependencies:
|
16
16
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- !ruby/object:Gem::Dependency
|
17
17
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name: bundler
|
@@ -41,6 +41,40 @@ dependencies:
|
|
41
41
|
- - "~>"
|
42
42
|
- !ruby/object:Gem::Version
|
43
43
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version: '10.3'
|
44
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+
- !ruby/object:Gem::Dependency
|
45
|
+
name: yard
|
46
|
+
requirement: !ruby/object:Gem::Requirement
|
47
|
+
requirements:
|
48
|
+
- - "~>"
|
49
|
+
- !ruby/object:Gem::Version
|
50
|
+
version: '0.8'
|
51
|
+
type: :development
|
52
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+
prerelease: false
|
53
|
+
version_requirements: !ruby/object:Gem::Requirement
|
54
|
+
requirements:
|
55
|
+
- - "~>"
|
56
|
+
- !ruby/object:Gem::Version
|
57
|
+
version: '0.8'
|
58
|
+
- !ruby/object:Gem::Dependency
|
59
|
+
name: codeclimate-test-reporter
|
60
|
+
requirement: !ruby/object:Gem::Requirement
|
61
|
+
requirements:
|
62
|
+
- - "~>"
|
63
|
+
- !ruby/object:Gem::Version
|
64
|
+
version: '0.4'
|
65
|
+
- - ">="
|
66
|
+
- !ruby/object:Gem::Version
|
67
|
+
version: 0.4.7
|
68
|
+
type: :development
|
69
|
+
prerelease: false
|
70
|
+
version_requirements: !ruby/object:Gem::Requirement
|
71
|
+
requirements:
|
72
|
+
- - "~>"
|
73
|
+
- !ruby/object:Gem::Version
|
74
|
+
version: '0.4'
|
75
|
+
- - ">="
|
76
|
+
- !ruby/object:Gem::Version
|
77
|
+
version: 0.4.7
|
44
78
|
- !ruby/object:Gem::Dependency
|
45
79
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name: bio
|
46
80
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requirement: !ruby/object:Gem::Requirement
|
@@ -87,18 +121,19 @@ dependencies:
|
|
87
121
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name: statsample
|
88
122
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requirement: !ruby/object:Gem::Requirement
|
89
123
|
requirements:
|
90
|
-
- -
|
124
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+
- - '='
|
91
125
|
- !ruby/object:Gem::Version
|
92
126
|
version: '1.4'
|
93
127
|
type: :runtime
|
94
128
|
prerelease: false
|
95
129
|
version_requirements: !ruby/object:Gem::Requirement
|
96
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|
requirements:
|
97
|
-
- -
|
131
|
+
- - '='
|
98
132
|
- !ruby/object:Gem::Version
|
99
133
|
version: '1.4'
|
100
|
-
description:
|
101
|
-
|
134
|
+
description: The tool validates the input predicted genes and provides useful information
|
135
|
+
(length validation, gene merge validation, sequence duplication checking, ORF finding)
|
136
|
+
based on the similarities to genes in public databases.
|
102
137
|
email: y.wurm@qmul.ac.uk
|
103
138
|
executables:
|
104
139
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- genevalidator
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