genevalidator 1.6.1 → 1.6.2
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- checksums.yaml +4 -4
- data/.gitignore +3 -1
- data/.travis.yml +2 -0
- data/README.md +78 -30
- data/Rakefile +11 -8
- data/aux/app_template_footer.erb +1 -6
- data/aux/app_template_header.erb +12 -32
- data/aux/files/css/style.css +2 -8
- data/aux/files/js/plots.js +564 -576
- data/aux/files/js/script.js +10 -0
- data/aux/json_footer.erb +8 -0
- data/aux/json_header.erb +19 -0
- data/aux/json_query.erb +14 -0
- data/aux/template_footer.erb +9 -58
- data/aux/template_header.erb +18 -58
- data/aux/template_query.erb +8 -36
- data/bin/genevalidator +45 -32
- data/genevalidator.gemspec +11 -7
- data/lib/genevalidator.rb +75 -455
- data/lib/genevalidator/arg_validation.rb +78 -107
- data/lib/genevalidator/blast.rb +57 -60
- data/lib/genevalidator/clusterization.rb +15 -15
- data/lib/genevalidator/exceptions.rb +32 -5
- data/lib/genevalidator/get_raw_sequences.rb +70 -33
- data/lib/genevalidator/hsp.rb +1 -4
- data/lib/genevalidator/json_to_gv_results.rb +109 -0
- data/lib/genevalidator/output.rb +177 -185
- data/lib/genevalidator/pool.rb +2 -1
- data/lib/genevalidator/sequences.rb +3 -3
- data/lib/genevalidator/tabular_parser.rb +24 -18
- data/lib/genevalidator/validation.rb +279 -0
- data/lib/genevalidator/validation_alignment.rb +31 -47
- data/lib/genevalidator/validation_blast_reading_frame.rb +19 -18
- data/lib/genevalidator/validation_duplication.rb +23 -19
- data/lib/genevalidator/validation_gene_merge.rb +30 -65
- data/lib/genevalidator/validation_length_cluster.rb +14 -53
- data/lib/genevalidator/validation_length_rank.rb +10 -11
- data/lib/genevalidator/validation_open_reading_frame.rb +18 -19
- data/lib/genevalidator/validation_report.rb +2 -5
- data/lib/genevalidator/validation_test.rb +8 -4
- data/lib/genevalidator/version.rb +1 -1
- data/test/test_all_validations.rb +51 -66
- data/test/test_blast.rb +68 -51
- data/test/test_clusterization.rb +1 -1
- data/test/test_clusterization_2d.rb +19 -13
- data/test/test_extended_array_methods.rb +1 -1
- data/test/test_files/all_validations_mrna/mrna.blast_tab6 +1806 -0
- data/test/test_files/all_validations_mrna/mrna.blast_tab7 +1865 -0
- data/test/test_files/all_validations_mrna/{all_validations_mrna.fasta.blast_xml → mrna.blast_xml} +18642 -1
- data/test/test_files/all_validations_mrna/{all_validations_mrna.fasta.blast_xml.index → mrna.blast_xml.index} +300 -0
- data/test/test_files/all_validations_mrna/{all_validations_mrna.fasta → mrna.fa} +0 -0
- data/test/test_files/all_validations_mrna/mrna.raw_seq +3970 -0
- data/test/test_files/all_validations_mrna/{all_validations_mrna.fasta.blast_xml.raw_seq.idx → mrna.raw_seq.idx} +901 -1
- data/test/test_files/all_validations_prot/{all_validations_prot.fasta.blast_tab → prot.blast_tab6} +416 -0
- data/test/test_files/all_validations_prot/prot.blast_tab7 +2400 -0
- data/test/test_files/all_validations_prot/{all_validations_prot.fasta.blast_xml → prot.blast_xml} +18299 -6723
- data/test/test_files/all_validations_prot/{all_validations_prot.fasta.blast_xml.index → prot.blast_xml.index} +408 -0
- data/test/test_files/all_validations_prot/{all_validations_prot.fasta → prot.fa} +0 -0
- data/test/test_files/all_validations_prot/{all_validations_prot.fasta.blast_xml.raw_seq → prot.raw_seq} +2735 -0
- data/test/test_files/all_validations_prot/{all_validations_prot.fasta.blast_xml.raw_seq.idx → prot.raw_seq.idx} +3032 -1808
- data/test/test_sequences.rb +46 -41
- data/test/test_validation_open_reading_frame.rb +318 -202
- data/test/test_validations.rb +48 -32
- metadata +76 -102
- data/doc/AliasDuplicationError.html +0 -134
- data/doc/AlignmentValidation.html +0 -1687
- data/doc/AlignmentValidationOutput.html +0 -659
- data/doc/Blast.html +0 -1905
- data/doc/BlastRFValidationOutput.html +0 -545
- data/doc/BlastReadingFrameValidation.html +0 -370
- data/doc/BlastUtils.html +0 -875
- data/doc/ClasspathError.html +0 -134
- data/doc/Cluster.html +0 -1316
- data/doc/DuplciationValidationOutput.html +0 -564
- data/doc/DuplicationValidation.html +0 -920
- data/doc/DuplicationValidationOutput.html +0 -564
- data/doc/FileNotFoundException.html +0 -134
- data/doc/GeneMergeValidation.html +0 -935
- data/doc/GeneMergeValidationOutput.html +0 -652
- data/doc/HierarchicalClusterization.html +0 -994
- data/doc/Hsp.html +0 -1485
- data/doc/InconsistentTabularFormat.html +0 -135
- data/doc/LengthClusterValidation.html +0 -982
- data/doc/LengthClusterValidationOutput.html +0 -515
- data/doc/LengthRankValidation.html +0 -496
- data/doc/LengthRankValidationOutput.html +0 -517
- data/doc/NoInternetError.html +0 -135
- data/doc/NoMafftInstallationError.html +0 -134
- data/doc/NoPIdentError.html +0 -134
- data/doc/NoValidationError.html +0 -134
- data/doc/NotEnoughHitsError.html +0 -135
- data/doc/ORFValidationOutput.html +0 -593
- data/doc/OpenReadingFrameValidation.html +0 -1107
- data/doc/OtherError.html +0 -123
- data/doc/Output.html +0 -1540
- data/doc/Pair.html +0 -309
- data/doc/PairCluster.html +0 -767
- data/doc/Plot.html +0 -837
- data/doc/QueryError.html +0 -134
- data/doc/ReportClassError.html +0 -135
- data/doc/Sequence.html +0 -1299
- data/doc/SequenceTypeError.html +0 -135
- data/doc/TabularEntry.html +0 -837
- data/doc/TabularParser.html +0 -1104
- data/doc/Validation.html +0 -2147
- data/doc/ValidationClassError.html +0 -134
- data/doc/ValidationOutput.html +0 -460
- data/doc/ValidationReport.html +0 -940
- data/doc/ValidationTest.html +0 -939
- data/doc/_index.html +0 -449
- data/doc/class_list.html +0 -54
- data/doc/css/common.css +0 -1
- data/doc/css/full_list.css +0 -57
- data/doc/css/style.css +0 -338
- data/doc/file.README.html +0 -151
- data/doc/file_list.html +0 -56
- data/doc/frames.html +0 -26
- data/doc/index.html +0 -151
- data/doc/js/app.js +0 -214
- data/doc/js/full_list.js +0 -178
- data/doc/js/jquery.js +0 -4
- data/doc/method_list.html +0 -1505
- data/doc/top-level-namespace.html +0 -112
- data/test/test_files/all_validations_mrna/all_validations_mrna.fasta.blast_tab +0 -967
- data/test/test_files/all_validations_mrna/all_validations_mrna.fasta.blast_tab.index +0 -967
- data/test/test_files/all_validations_mrna/all_validations_mrna.fasta.blast_tab.raw_seq +0 -4929
- data/test/test_files/all_validations_mrna/all_validations_mrna.fasta.blast_tab.raw_seq.idx +0 -1006
- data/test/test_files/all_validations_mrna/all_validations_mrna.fasta.blast_xml.raw_seq +0 -2075
- data/test/test_files/all_validations_prot/all_validations_prot.fasta.blast_tab.index +0 -1864
- data/test/test_files/all_validations_prot/all_validations_prot.fasta.blast_tab.raw_seq +0 -42411
- data/test/test_files/all_validations_prot/all_validations_prot.fasta.blast_tab.raw_seq.idx +0 -3751
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<p>Initilizes the object Params: <tt>type</tt>: type of the predicted sequence
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<span class='kw'>def</span> <span class='id identifier rubyid_initialize'>initialize</span><span class='lparen'>(</span><span class='id identifier rubyid_type'>type</span><span class='comma'>,</span> <span class='id identifier rubyid_prediction'>prediction</span><span class='comma'>,</span> <span class='id identifier rubyid_hits'>hits</span><span class='comma'>,</span> <span class='id identifier rubyid_filename'>filename</span><span class='rparen'>)</span>
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<span class='ivar'>@header</span> <span class='op'>=</span> <span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>Length Cluster</span><span class='tstring_end'>"</span></span>
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<span class='ivar'>@description</span> <span class='op'>=</span> <span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>Check whether the prediction length fits most of the BLAST hit lengths,</span><span class='tstring_end'>"</span></span><span class='op'><<</span>
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<span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'> by 1D hierarchical clusterization. Meaning of the output displayed: Prediction_len</span><span class='tstring_end'>"</span></span><span class='op'><<</span>
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<span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'> [Main Cluster Length Interval]</span><span class='tstring_end'>"</span></span>
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<span class='ivar'>@cli_name</span> <span class='op'>=</span> <span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>lenc</span><span class='tstring_end'>"</span></span>
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<pre class="code"><span class="info file"># File 'lib/genevalidator/validation_length_cluster.rb', line 42</span>
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<span class='kw'>end</span></pre>
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<pre class="code"><span class="info file"># File 'lib/genevalidator/validation_length_cluster.rb', line 41</span>
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<span class='kw'>def</span> <span class='id identifier rubyid_max_density_cluster'>max_density_cluster</span>
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<span class='ivar'>@max_density_cluster</span>
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<h3 class="signature first" id="clusterization_by_length-instance_method">
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<p>Clusterization by length from a list of sequences Params:</p>
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<table class="rdoc-list"><tr><td class="rdoc-term"><p><tt>debug</tt> (optional)</p></td>
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<p>true to display debug information, false by default (optional argument)</p>
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<p>array of <tt>Sequence</tt> objects</p>
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</td></tr><tr><td class="rdoc-term"><p><tt>predicted_seq</tt></p></td>
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<p><tt>Sequence</tt> objetc</p>
|
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</td></tr></table>
|
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<p>Output</p>
|
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<table class="rdoc-list"><tr><td class="rdoc-term"><p>output 1</p></td>
|
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|
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<td>
|
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|
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<p>array of Cluster objects</p>
|
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</td></tr><tr><td class="rdoc-term"><p>output 2</p></td>
|
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<td>
|
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<p>the index of the most dense cluster</p>
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</td></tr></table>
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<pre class="code"><span class="info file"># File 'lib/genevalidator/validation_length_cluster.rb', line 115</span>
|
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|
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|
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<span class='kw'>def</span> <span class='id identifier rubyid_clusterization_by_length'>clusterization_by_length</span><span class='lparen'>(</span><span class='id identifier rubyid_debug'>debug</span> <span class='op'>=</span> <span class='kw'>false</span><span class='comma'>,</span>
|
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<span class='id identifier rubyid_lst'>lst</span> <span class='op'>=</span> <span class='ivar'>@hits</span><span class='comma'>,</span>
|
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|
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<span class='id identifier rubyid_predicted_seq'>predicted_seq</span> <span class='op'>=</span> <span class='ivar'>@prediction</span><span class='rparen'>)</span>
|
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|
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<span class='kw'>begin</span>
|
669
|
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<span class='id identifier rubyid_raise'>raise</span> <span class='const'>TypeError</span> <span class='kw'>unless</span> <span class='id identifier rubyid_lst'>lst</span><span class='lbracket'>[</span><span class='int'>0</span><span class='rbracket'>]</span><span class='period'>.</span><span class='id identifier rubyid_is_a?'>is_a?</span> <span class='const'>Sequence</span> <span class='kw'>and</span>
|
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|
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<span class='id identifier rubyid_predicted_seq'>predicted_seq</span><span class='period'>.</span><span class='id identifier rubyid_is_a?'>is_a?</span> <span class='const'>Sequence</span>
|
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|
-
|
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|
-
<span class='id identifier rubyid_contents'>contents</span> <span class='op'>=</span> <span class='id identifier rubyid_lst'>lst</span><span class='period'>.</span><span class='id identifier rubyid_map'>map</span><span class='lbrace'>{</span> <span class='op'>|</span><span class='id identifier rubyid_x'>x</span><span class='op'>|</span> <span class='id identifier rubyid_x'>x</span><span class='period'>.</span><span class='id identifier rubyid_length_protein'>length_protein</span><span class='period'>.</span><span class='id identifier rubyid_to_i'>to_i</span> <span class='rbrace'>}</span><span class='period'>.</span><span class='id identifier rubyid_sort'>sort</span><span class='lbrace'>{</span><span class='op'>|</span><span class='id identifier rubyid_a'>a</span><span class='comma'>,</span><span class='id identifier rubyid_b'>b</span><span class='op'>|</span> <span class='id identifier rubyid_a'>a</span><span class='op'><=></span><span class='id identifier rubyid_b'>b</span><span class='rbrace'>}</span>
|
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|
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|
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|
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<span class='id identifier rubyid_hc'>hc</span> <span class='op'>=</span> <span class='const'>HierarchicalClusterization</span><span class='period'>.</span><span class='id identifier rubyid_new'>new</span><span class='lparen'>(</span><span class='id identifier rubyid_contents'>contents</span><span class='rparen'>)</span>
|
675
|
-
<span class='id identifier rubyid_clusters'>clusters</span> <span class='op'>=</span> <span class='id identifier rubyid_hc'>hc</span><span class='period'>.</span><span class='id identifier rubyid_hierarchical_clusterization'>hierarchical_clusterization</span>
|
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|
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|
677
|
-
<span class='id identifier rubyid_max_density'>max_density</span> <span class='op'>=</span> <span class='int'>0</span><span class='semicolon'>;</span>
|
678
|
-
<span class='id identifier rubyid_max_density_cluster_idx'>max_density_cluster_idx</span> <span class='op'>=</span> <span class='int'>0</span><span class='semicolon'>;</span>
|
679
|
-
<span class='id identifier rubyid_clusters'>clusters</span><span class='period'>.</span><span class='id identifier rubyid_each_with_index'>each_with_index</span> <span class='kw'>do</span> <span class='op'>|</span><span class='id identifier rubyid_item'>item</span><span class='comma'>,</span> <span class='id identifier rubyid_i'>i</span><span class='op'>|</span>
|
680
|
-
<span class='kw'>if</span> <span class='id identifier rubyid_item'>item</span><span class='period'>.</span><span class='id identifier rubyid_density'>density</span> <span class='op'>></span> <span class='id identifier rubyid_max_density'>max_density</span>
|
681
|
-
<span class='id identifier rubyid_max_density'>max_density</span> <span class='op'>=</span> <span class='id identifier rubyid_item'>item</span><span class='period'>.</span><span class='id identifier rubyid_density'>density</span>
|
682
|
-
<span class='id identifier rubyid_max_density_cluster_idx'>max_density_cluster_idx</span> <span class='op'>=</span> <span class='id identifier rubyid_i'>i</span><span class='semicolon'>;</span>
|
683
|
-
<span class='kw'>end</span>
|
684
|
-
<span class='kw'>end</span>
|
685
|
-
|
686
|
-
<span class='kw'>return</span> <span class='lbracket'>[</span><span class='id identifier rubyid_clusters'>clusters</span><span class='comma'>,</span> <span class='id identifier rubyid_max_density_cluster_idx'>max_density_cluster_idx</span><span class='rbracket'>]</span>
|
687
|
-
|
688
|
-
<span class='kw'>rescue</span> <span class='const'>TypeError</span> <span class='op'>=></span> <span class='id identifier rubyid_error'>error</span>
|
689
|
-
<span class='gvar'>$stderr</span><span class='period'>.</span><span class='id identifier rubyid_print'>print</span> <span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>Type error at </span><span class='embexpr_beg'>#{</span><span class='id identifier rubyid_error'>error</span><span class='period'>.</span><span class='id identifier rubyid_backtrace'>backtrace</span><span class='lbracket'>[</span><span class='int'>0</span><span class='rbracket'>]</span><span class='period'>.</span><span class='id identifier rubyid_scan'>scan</span><span class='lparen'>(</span><span class='tstring'><span class='regexp_beg'>/</span><span class='tstring_content'>\/([^\/]+:\d+):.*</span><span class='regexp_end'>/</span></span><span class='rparen'>)</span><span class='lbracket'>[</span><span class='int'>0</span><span class='rbracket'>]</span><span class='lbracket'>[</span><span class='int'>0</span><span class='rbracket'>]</span><span class='rbrace'>}</span><span class='tstring_content'>.</span><span class='tstring_end'>"</span></span><span class='op'><<</span>
|
690
|
-
<span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'> Possible cause: one of the arguments of 'clusterization_by_length'</span><span class='tstring_end'>"</span></span><span class='op'><<</span>
|
691
|
-
<span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'> method has not the proper type.\n</span><span class='tstring_end'>"</span></span>
|
692
|
-
<span class='id identifier rubyid_exit'>exit</span>
|
693
|
-
<span class='kw'>end</span>
|
694
|
-
<span class='kw'>end</span></pre>
|
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|
-
</td>
|
696
|
-
</tr>
|
697
|
-
</table>
|
698
|
-
</div>
|
699
|
-
|
700
|
-
<div class="method_details ">
|
701
|
-
<h3 class="signature " id="plot_histo_clusters-instance_method">
|
702
|
-
|
703
|
-
- (<tt>Object</tt>) <strong>plot_histo_clusters</strong>(output = "#{@filename}_len_clusters.json", clusters = @clusters, max_density_cluster = @max_density_cluster, prediction = @prediction)
|
704
|
-
|
705
|
-
|
706
|
-
|
707
|
-
|
708
|
-
|
709
|
-
</h3><div class="docstring">
|
710
|
-
<div class="discussion">
|
711
|
-
|
712
|
-
<p>Generates a json file containing data used for plotting the histogram of
|
713
|
-
the length distribution given a lust of Cluster objects <tt>output</tt>:
|
714
|
-
filename where to save the graph <tt>clusters</tt>: array of
|
715
|
-
<tt>Cluster</tt> objects <tt>max_density_cluster</tt>: index of the most
|
716
|
-
dense cluster <tt>prediction</tt>: <tt>Sequence</tt> object Output:
|
717
|
-
<tt>Plot</tt> object</p>
|
718
|
-
|
719
|
-
|
720
|
-
</div>
|
721
|
-
</div>
|
722
|
-
<div class="tags">
|
723
|
-
|
724
|
-
|
725
|
-
</div><table class="source_code">
|
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|
-
<tr>
|
727
|
-
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|
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|
-
<pre class="lines">
|
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|
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|
-
173</pre>
|
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|
-
</td>
|
751
|
-
<td>
|
752
|
-
<pre class="code"><span class="info file"># File 'lib/genevalidator/validation_length_cluster.rb', line 155</span>
|
753
|
-
|
754
|
-
<span class='kw'>def</span> <span class='id identifier rubyid_plot_histo_clusters'>plot_histo_clusters</span><span class='lparen'>(</span><span class='id identifier rubyid_output'>output</span> <span class='op'>=</span> <span class='tstring'><span class='tstring_beg'>"</span><span class='embexpr_beg'>#{</span><span class='ivar'>@filename</span><span class='rbrace'>}</span><span class='tstring_content'>_len_clusters.json</span><span class='tstring_end'>"</span></span><span class='comma'>,</span>
|
755
|
-
<span class='id identifier rubyid_clusters'>clusters</span> <span class='op'>=</span> <span class='ivar'>@clusters</span><span class='comma'>,</span>
|
756
|
-
<span class='id identifier rubyid_max_density_cluster'>max_density_cluster</span> <span class='op'>=</span> <span class='ivar'>@max_density_cluster</span><span class='comma'>,</span>
|
757
|
-
<span class='id identifier rubyid_prediction'>prediction</span> <span class='op'>=</span> <span class='ivar'>@prediction</span><span class='rparen'>)</span>
|
758
|
-
|
759
|
-
<span class='id identifier rubyid_f'>f</span> <span class='op'>=</span> <span class='const'>File</span><span class='period'>.</span><span class='id identifier rubyid_open'>open</span><span class='lparen'>(</span><span class='id identifier rubyid_output'>output</span><span class='comma'>,</span> <span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>w</span><span class='tstring_end'>"</span></span><span class='rparen'>)</span>
|
760
|
-
<span class='id identifier rubyid_f'>f</span><span class='period'>.</span><span class='id identifier rubyid_write'>write</span><span class='lparen'>(</span><span class='id identifier rubyid_clusters'>clusters</span><span class='period'>.</span><span class='id identifier rubyid_each_with_index'>each_with_index</span><span class='period'>.</span><span class='id identifier rubyid_map'>map</span><span class='lbrace'>{</span><span class='op'>|</span><span class='id identifier rubyid_cluster'>cluster</span><span class='comma'>,</span> <span class='id identifier rubyid_i'>i</span><span class='op'>|</span>
|
761
|
-
<span class='id identifier rubyid_cluster'>cluster</span><span class='period'>.</span><span class='id identifier rubyid_lengths'>lengths</span><span class='period'>.</span><span class='id identifier rubyid_collect'>collect</span><span class='lbrace'>{</span><span class='op'>|</span><span class='id identifier rubyid_k'>k</span><span class='comma'>,</span><span class='id identifier rubyid_v'>v</span><span class='op'>|</span>
|
762
|
-
<span class='lbrace'>{</span><span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>key</span><span class='tstring_end'>"</span></span><span class='op'>=></span><span class='id identifier rubyid_k'>k</span><span class='comma'>,</span> <span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>value</span><span class='tstring_end'>"</span></span><span class='op'>=></span><span class='id identifier rubyid_v'>v</span><span class='comma'>,</span> <span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>main</span><span class='tstring_end'>"</span></span><span class='op'>=></span><span class='lparen'>(</span><span class='id identifier rubyid_i'>i</span><span class='op'>==</span><span class='id identifier rubyid_max_density_cluster'>max_density_cluster</span><span class='rparen'>)</span><span class='rbrace'>}</span>
|
763
|
-
<span class='rbrace'>}</span><span class='rbrace'>}</span><span class='period'>.</span><span class='id identifier rubyid_to_json'>to_json</span><span class='rparen'>)</span>
|
764
|
-
<span class='id identifier rubyid_f'>f</span><span class='period'>.</span><span class='id identifier rubyid_close'>close</span>
|
765
|
-
<span class='const'>Plot</span><span class='period'>.</span><span class='id identifier rubyid_new'>new</span><span class='lparen'>(</span><span class='id identifier rubyid_output'>output</span><span class='period'>.</span><span class='id identifier rubyid_scan'>scan</span><span class='lparen'>(</span><span class='tstring'><span class='regexp_beg'>/</span><span class='tstring_content'>\/([^\/]+)$</span><span class='regexp_end'>/</span></span><span class='rparen'>)</span><span class='lbracket'>[</span><span class='int'>0</span><span class='rbracket'>]</span><span class='lbracket'>[</span><span class='int'>0</span><span class='rbracket'>]</span><span class='comma'>,</span>
|
766
|
-
<span class='symbol'>:bars</span><span class='comma'>,</span>
|
767
|
-
<span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>Length distribution histogram</span><span class='tstring_end'>"</span></span><span class='comma'>,</span>
|
768
|
-
<span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>prediction, black;most dense cluster,red;other hits, blue</span><span class='tstring_end'>"</span></span><span class='comma'>,</span>
|
769
|
-
<span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>length</span><span class='tstring_end'>"</span></span><span class='comma'>,</span>
|
770
|
-
<span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>frequency</span><span class='tstring_end'>"</span></span><span class='comma'>,</span>
|
771
|
-
<span class='id identifier rubyid_prediction'>prediction</span><span class='period'>.</span><span class='id identifier rubyid_length_protein'>length_protein</span><span class='rparen'>)</span>
|
772
|
-
<span class='kw'>end</span></pre>
|
773
|
-
</td>
|
774
|
-
</tr>
|
775
|
-
</table>
|
776
|
-
</div>
|
777
|
-
|
778
|
-
<div class="method_details ">
|
779
|
-
<h3 class="signature " id="plot_len_clusters-instance_method">
|
780
|
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|
781
|
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- (<tt>Object</tt>) <strong>plot_len_clusters</strong>(output = "#{@filename}_len.json", hits = @hits)
|
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|
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|
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|
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</h3><div class="docstring">
|
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|
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|
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<p>Generates a json file cotaining data used for plotting lines corresponding
|
791
|
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to the start and end hit offsets Params: <tt>output</tt>: filename where to
|
792
|
-
save the graph <tt>hits</tt>: array of Sequence objects Output:
|
793
|
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<tt>Plot</tt> object</p>
|
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</div>
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<pre class="code"><span class="info file"># File 'lib/genevalidator/validation_length_cluster.rb', line 183</span>
|
834
|
-
|
835
|
-
<span class='kw'>def</span> <span class='id identifier rubyid_plot_len_clusters'>plot_len_clusters</span><span class='lparen'>(</span><span class='id identifier rubyid_output'>output</span> <span class='op'>=</span> <span class='tstring'><span class='tstring_beg'>"</span><span class='embexpr_beg'>#{</span><span class='ivar'>@filename</span><span class='rbrace'>}</span><span class='tstring_content'>_len.json</span><span class='tstring_end'>"</span></span><span class='comma'>,</span> <span class='id identifier rubyid_hits'>hits</span> <span class='op'>=</span> <span class='ivar'>@hits</span><span class='rparen'>)</span>
|
836
|
-
|
837
|
-
<span class='id identifier rubyid_f'>f</span> <span class='op'>=</span> <span class='const'>File</span><span class='period'>.</span><span class='id identifier rubyid_open'>open</span><span class='lparen'>(</span><span class='id identifier rubyid_output'>output</span> <span class='comma'>,</span> <span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>w</span><span class='tstring_end'>"</span></span><span class='rparen'>)</span>
|
838
|
-
<span class='id identifier rubyid_lst'>lst</span> <span class='op'>=</span> <span class='ivar'>@hits</span><span class='period'>.</span><span class='id identifier rubyid_sort'>sort</span><span class='lbrace'>{</span><span class='op'>|</span><span class='id identifier rubyid_a'>a</span><span class='comma'>,</span><span class='id identifier rubyid_b'>b</span><span class='op'>|</span> <span class='id identifier rubyid_a'>a</span><span class='period'>.</span><span class='id identifier rubyid_length_protein'>length_protein</span><span class='op'><=></span><span class='id identifier rubyid_b'>b</span><span class='period'>.</span><span class='id identifier rubyid_length_protein'>length_protein</span><span class='rbrace'>}</span>
|
839
|
-
|
840
|
-
<span class='id identifier rubyid_no_lines'>no_lines</span> <span class='op'>=</span> <span class='int'>100</span>
|
841
|
-
<span class='id identifier rubyid_ratio'>ratio</span> <span class='op'>=</span> <span class='lparen'>(</span><span class='id identifier rubyid_hits'>hits</span><span class='period'>.</span><span class='id identifier rubyid_length'>length</span><span class='op'>/</span><span class='id identifier rubyid_no_lines'>no_lines</span><span class='rparen'>)</span><span class='period'>.</span><span class='id identifier rubyid_to_i'>to_i</span>
|
842
|
-
<span class='kw'>if</span> <span class='id identifier rubyid_ratio'>ratio</span> <span class='op'>==</span> <span class='int'>0</span>
|
843
|
-
<span class='id identifier rubyid_f'>f</span><span class='period'>.</span><span class='id identifier rubyid_write'>write</span><span class='lparen'>(</span><span class='lparen'>(</span><span class='id identifier rubyid_lst'>lst</span><span class='period'>.</span><span class='id identifier rubyid_each_with_index'>each_with_index</span><span class='period'>.</span><span class='id identifier rubyid_map'>map</span><span class='lbrace'>{</span><span class='op'>|</span><span class='id identifier rubyid_hit'>hit</span><span class='comma'>,</span> <span class='id identifier rubyid_i'>i</span><span class='op'>|</span> <span class='lbrace'>{</span><span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>y</span><span class='tstring_end'>"</span></span><span class='op'>=></span><span class='id identifier rubyid_i'>i</span><span class='comma'>,</span> <span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>start</span><span class='tstring_end'>"</span></span><span class='op'>=></span><span class='int'>0</span><span class='comma'>,</span> <span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>stop</span><span class='tstring_end'>"</span></span><span class='op'>=></span><span class='id identifier rubyid_hit'>hit</span><span class='period'>.</span><span class='id identifier rubyid_length_protein'>length_protein</span><span class='comma'>,</span> <span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>color</span><span class='tstring_end'>"</span></span><span class='op'>=></span><span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>gray</span><span class='tstring_end'>"</span></span><span class='rbrace'>}</span><span class='rbrace'>}</span> <span class='op'>+</span>
|
844
|
-
<span class='id identifier rubyid_lst'>lst</span><span class='period'>.</span><span class='id identifier rubyid_each_with_index'>each_with_index</span><span class='period'>.</span><span class='id identifier rubyid_map'>map</span><span class='lbrace'>{</span><span class='op'>|</span><span class='id identifier rubyid_hit'>hit</span><span class='comma'>,</span> <span class='id identifier rubyid_i'>i</span><span class='op'>|</span> <span class='id identifier rubyid_hit'>hit</span><span class='period'>.</span><span class='id identifier rubyid_hsp_list'>hsp_list</span><span class='period'>.</span><span class='id identifier rubyid_map'>map</span><span class='lbrace'>{</span><span class='op'>|</span><span class='id identifier rubyid_hsp'>hsp</span><span class='op'>|</span> <span class='lbrace'>{</span><span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>y</span><span class='tstring_end'>"</span></span><span class='op'>=></span><span class='id identifier rubyid_i'>i</span><span class='comma'>,</span> <span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>start</span><span class='tstring_end'>"</span></span><span class='op'>=></span><span class='id identifier rubyid_hsp'>hsp</span><span class='period'>.</span><span class='id identifier rubyid_hit_from'>hit_from</span><span class='comma'>,</span> <span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>stop</span><span class='tstring_end'>"</span></span><span class='op'>=></span><span class='id identifier rubyid_hsp'>hsp</span><span class='period'>.</span><span class='id identifier rubyid_hit_to'>hit_to</span><span class='comma'>,</span> <span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>color</span><span class='tstring_end'>"</span></span><span class='op'>=></span><span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>red</span><span class='tstring_end'>"</span></span><span class='rbrace'>}</span><span class='rbrace'>}</span><span class='rbrace'>}</span><span class='period'>.</span><span class='id identifier rubyid_flatten'>flatten</span><span class='rparen'>)</span><span class='period'>.</span><span class='id identifier rubyid_to_json'>to_json</span><span class='rparen'>)</span>
|
845
|
-
<span class='kw'>else</span>
|
846
|
-
<span class='id identifier rubyid_f'>f</span><span class='period'>.</span><span class='id identifier rubyid_write'>write</span><span class='lparen'>(</span><span class='lparen'>(</span><span class='id identifier rubyid_lst'>lst</span><span class='period'>.</span><span class='id identifier rubyid_select'>select</span><span class='period'>.</span><span class='id identifier rubyid_each_with_index'>each_with_index</span> <span class='lbrace'>{</span><span class='op'>|</span><span class='id identifier rubyid_hit'>hit</span><span class='comma'>,</span> <span class='id identifier rubyid_i'>i</span><span class='op'>|</span> <span class='id identifier rubyid_i'>i</span><span class='op'>%</span><span class='id identifier rubyid_ratio'>ratio</span><span class='op'>==</span><span class='int'>0</span><span class='rbrace'>}</span><span class='period'>.</span><span class='id identifier rubyid_each_with_index'>each_with_index</span><span class='period'>.</span><span class='id identifier rubyid_map'>map</span><span class='lbrace'>{</span><span class='op'>|</span><span class='id identifier rubyid_hit'>hit</span><span class='comma'>,</span> <span class='id identifier rubyid_i'>i</span><span class='op'>|</span> <span class='lbrace'>{</span><span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>y</span><span class='tstring_end'>"</span></span><span class='op'>=></span><span class='id identifier rubyid_i'>i</span><span class='comma'>,</span> <span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>start</span><span class='tstring_end'>"</span></span><span class='op'>=></span><span class='int'>0</span><span class='comma'>,</span> <span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>stop</span><span class='tstring_end'>"</span></span><span class='op'>=></span><span class='id identifier rubyid_hit'>hit</span><span class='period'>.</span><span class='id identifier rubyid_length_protein'>length_protein</span><span class='comma'>,</span> <span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>color</span><span class='tstring_end'>"</span></span><span class='op'>=></span><span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>gray</span><span class='tstring_end'>"</span></span><span class='rbrace'>}</span><span class='rbrace'>}</span> <span class='op'>+</span>
|
847
|
-
<span class='id identifier rubyid_lst'>lst</span><span class='period'>.</span><span class='id identifier rubyid_select'>select</span><span class='period'>.</span><span class='id identifier rubyid_each_with_index'>each_with_index</span> <span class='lbrace'>{</span><span class='op'>|</span><span class='id identifier rubyid_hit'>hit</span><span class='comma'>,</span> <span class='id identifier rubyid_i'>i</span><span class='op'>|</span> <span class='id identifier rubyid_i'>i</span><span class='op'>%</span><span class='id identifier rubyid_ratio'>ratio</span><span class='op'>==</span><span class='int'>0</span><span class='rbrace'>}</span><span class='period'>.</span><span class='id identifier rubyid_each_with_index'>each_with_index</span><span class='period'>.</span><span class='id identifier rubyid_map'>map</span><span class='lbrace'>{</span><span class='op'>|</span><span class='id identifier rubyid_hit'>hit</span><span class='comma'>,</span> <span class='id identifier rubyid_i'>i</span><span class='op'>|</span> <span class='id identifier rubyid_hit'>hit</span><span class='period'>.</span><span class='id identifier rubyid_hsp_list'>hsp_list</span><span class='period'>.</span><span class='id identifier rubyid_map'>map</span><span class='lbrace'>{</span><span class='op'>|</span><span class='id identifier rubyid_hsp'>hsp</span><span class='op'>|</span> <span class='lbrace'>{</span><span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>y</span><span class='tstring_end'>"</span></span><span class='op'>=></span><span class='id identifier rubyid_i'>i</span><span class='comma'>,</span> <span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>start</span><span class='tstring_end'>"</span></span><span class='op'>=></span><span class='id identifier rubyid_hsp'>hsp</span><span class='period'>.</span><span class='id identifier rubyid_hit_from'>hit_from</span><span class='comma'>,</span> <span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>stop</span><span class='tstring_end'>"</span></span><span class='op'>=></span><span class='id identifier rubyid_hsp'>hsp</span><span class='period'>.</span><span class='id identifier rubyid_hit_to'>hit_to</span><span class='comma'>,</span> <span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>color</span><span class='tstring_end'>"</span></span><span class='op'>=></span><span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>red</span><span class='tstring_end'>"</span></span><span class='rbrace'>}</span><span class='rbrace'>}</span><span class='rbrace'>}</span><span class='period'>.</span><span class='id identifier rubyid_flatten'>flatten</span><span class='rparen'>)</span><span class='period'>.</span><span class='id identifier rubyid_to_json'>to_json</span><span class='rparen'>)</span>
|
848
|
-
<span class='kw'>end</span>
|
849
|
-
|
850
|
-
<span class='id identifier rubyid_f'>f</span><span class='period'>.</span><span class='id identifier rubyid_close'>close</span>
|
851
|
-
<span class='const'>Plot</span><span class='period'>.</span><span class='id identifier rubyid_new'>new</span><span class='lparen'>(</span><span class='id identifier rubyid_output'>output</span><span class='period'>.</span><span class='id identifier rubyid_scan'>scan</span><span class='lparen'>(</span><span class='tstring'><span class='regexp_beg'>/</span><span class='tstring_content'>\/([^\/]+)$</span><span class='regexp_end'>/</span></span><span class='rparen'>)</span><span class='lbracket'>[</span><span class='int'>0</span><span class='rbracket'>]</span><span class='lbracket'>[</span><span class='int'>0</span><span class='rbracket'>]</span><span class='comma'>,</span>
|
852
|
-
<span class='symbol'>:lines</span><span class='comma'>,</span>
|
853
|
-
<span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>Hits vs prediction</span><span class='tstring_end'>"</span></span><span class='comma'>,</span>
|
854
|
-
<span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>hit length, gray;high-scoring segment pairs (hsp), red</span><span class='tstring_end'>"</span></span><span class='comma'>,</span>
|
855
|
-
<span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>length</span><span class='tstring_end'>"</span></span><span class='comma'>,</span>
|
856
|
-
<span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>idx</span><span class='tstring_end'>"</span></span><span class='comma'>,</span>
|
857
|
-
<span class='id identifier rubyid_hits'>hits</span><span class='period'>.</span><span class='id identifier rubyid_length'>length</span><span class='rparen'>)</span>
|
858
|
-
<span class='kw'>end</span></pre>
|
859
|
-
</td>
|
860
|
-
</tr>
|
861
|
-
</table>
|
862
|
-
</div>
|
863
|
-
|
864
|
-
<div class="method_details ">
|
865
|
-
<h3 class="signature " id="run-instance_method">
|
866
|
-
|
867
|
-
- (<tt>Object</tt>) <strong>run</strong>
|
868
|
-
|
869
|
-
|
870
|
-
|
871
|
-
|
872
|
-
|
873
|
-
</h3><div class="docstring">
|
874
|
-
<div class="discussion">
|
875
|
-
|
876
|
-
<p>Validates the length of the predicted gene by comparing the length of the
|
877
|
-
prediction to the most dense cluster The most dense cluster is obtained by
|
878
|
-
hierarchical clusterization Plots are generated if required (see
|
879
|
-
<tt>plot</tt> variable) Output: <tt>LengthClusterValidationOutput</tt>
|
880
|
-
object</p>
|
881
|
-
|
882
|
-
|
883
|
-
</div>
|
884
|
-
</div>
|
885
|
-
<div class="tags">
|
886
|
-
|
887
|
-
|
888
|
-
</div><table class="source_code">
|
889
|
-
<tr>
|
890
|
-
<td>
|
891
|
-
<pre class="lines">
|
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|
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|
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|
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|
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|
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|
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103</pre>
|
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|
-
</td>
|
929
|
-
<td>
|
930
|
-
<pre class="code"><span class="info file"># File 'lib/genevalidator/validation_length_cluster.rb', line 70</span>
|
931
|
-
|
932
|
-
<span class='kw'>def</span> <span class='id identifier rubyid_run'>run</span>
|
933
|
-
<span class='kw'>begin</span>
|
934
|
-
<span class='id identifier rubyid_raise'>raise</span> <span class='const'>NotEnoughHitsError</span> <span class='kw'>unless</span> <span class='id identifier rubyid_hits'>hits</span><span class='period'>.</span><span class='id identifier rubyid_length'>length</span> <span class='op'>>=</span> <span class='int'>5</span>
|
935
|
-
<span class='id identifier rubyid_raise'>raise</span> <span class='const'>Exception</span> <span class='kw'>unless</span> <span class='id identifier rubyid_prediction'>prediction</span><span class='period'>.</span><span class='id identifier rubyid_is_a?'>is_a?</span> <span class='const'>Sequence</span> <span class='kw'>and</span>
|
936
|
-
<span class='id identifier rubyid_hits'>hits</span><span class='lbracket'>[</span><span class='int'>0</span><span class='rbracket'>]</span><span class='period'>.</span><span class='id identifier rubyid_is_a?'>is_a?</span> <span class='const'>Sequence</span>
|
937
|
-
|
938
|
-
<span class='id identifier rubyid_start'>start</span> <span class='op'>=</span> <span class='const'>Time</span><span class='period'>.</span><span class='id identifier rubyid_now'>now</span>
|
939
|
-
<span class='comment'># get [clusters, max_density_cluster_idx]
|
940
|
-
</span> <span class='id identifier rubyid_clusterization'>clusterization</span> <span class='op'>=</span> <span class='id identifier rubyid_clusterization_by_length'>clusterization_by_length</span>
|
941
|
-
|
942
|
-
<span class='ivar'>@clusters</span> <span class='op'>=</span> <span class='id identifier rubyid_clusterization'>clusterization</span><span class='lbracket'>[</span><span class='int'>0</span><span class='rbracket'>]</span>
|
943
|
-
<span class='ivar'>@max_density_cluster</span> <span class='op'>=</span> <span class='id identifier rubyid_clusterization'>clusterization</span><span class='lbracket'>[</span><span class='int'>1</span><span class='rbracket'>]</span>
|
944
|
-
<span class='id identifier rubyid_limits'>limits</span> <span class='op'>=</span> <span class='ivar'>@clusters</span><span class='lbracket'>[</span><span class='ivar'>@max_density_cluster</span><span class='rbracket'>]</span><span class='period'>.</span><span class='id identifier rubyid_get_limits'>get_limits</span>
|
945
|
-
<span class='id identifier rubyid_prediction_len'>prediction_len</span> <span class='op'>=</span> <span class='ivar'>@prediction</span><span class='period'>.</span><span class='id identifier rubyid_length_protein'>length_protein</span>
|
946
|
-
|
947
|
-
<span class='ivar'>@validation_report</span> <span class='op'>=</span> <span class='const'>LengthClusterValidationOutput</span><span class='period'>.</span><span class='id identifier rubyid_new'>new</span><span class='lparen'>(</span><span class='id identifier rubyid_prediction_len'>prediction_len</span><span class='comma'>,</span> <span class='id identifier rubyid_limits'>limits</span><span class='rparen'>)</span>
|
948
|
-
<span class='id identifier rubyid_plot1'>plot1</span> <span class='op'>=</span> <span class='id identifier rubyid_plot_histo_clusters'>plot_histo_clusters</span>
|
949
|
-
<span class='ivar'>@validation_report</span><span class='period'>.</span><span class='id identifier rubyid_plot_files'>plot_files</span><span class='period'>.</span><span class='id identifier rubyid_push'>push</span><span class='lparen'>(</span><span class='id identifier rubyid_plot1'>plot1</span><span class='rparen'>)</span>
|
950
|
-
<span class='id identifier rubyid_plot2'>plot2</span> <span class='op'>=</span> <span class='id identifier rubyid_plot_len_clusters'>plot_len_clusters</span>
|
951
|
-
<span class='ivar'>@validation_report</span><span class='period'>.</span><span class='id identifier rubyid_plot_files'>plot_files</span><span class='period'>.</span><span class='id identifier rubyid_push'>push</span><span class='lparen'>(</span><span class='id identifier rubyid_plot2'>plot2</span><span class='rparen'>)</span>
|
952
|
-
<span class='ivar'>@running_time</span> <span class='op'>=</span> <span class='const'>Time</span><span class='period'>.</span><span class='id identifier rubyid_now'>now</span> <span class='op'>-</span> <span class='id identifier rubyid_start'>start</span>
|
953
|
-
|
954
|
-
<span class='kw'>return</span> <span class='ivar'>@validation_report</span>
|
955
|
-
|
956
|
-
<span class='comment'># Exception is raised when blast founds no hits
|
957
|
-
</span> <span class='kw'>rescue</span> <span class='const'>NotEnoughHitsError</span> <span class='op'>=></span> <span class='id identifier rubyid_error'>error</span>
|
958
|
-
<span class='ivar'>@validation_report</span> <span class='op'>=</span> <span class='const'>ValidationReport</span><span class='period'>.</span><span class='id identifier rubyid_new'>new</span><span class='lparen'>(</span><span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>Not enough evidence</span><span class='tstring_end'>"</span></span><span class='comma'>,</span> <span class='symbol'>:warning</span><span class='rparen'>)</span>
|
959
|
-
<span class='kw'>return</span> <span class='ivar'>@validation_report</span>
|
960
|
-
<span class='kw'>else</span>
|
961
|
-
<span class='ivar'>@validation_report</span> <span class='op'>=</span> <span class='const'>ValidationReport</span><span class='period'>.</span><span class='id identifier rubyid_new'>new</span><span class='lparen'>(</span><span class='tstring'><span class='tstring_beg'>"</span><span class='tstring_content'>Unexpected error</span><span class='tstring_end'>"</span></span><span class='comma'>,</span> <span class='symbol'>:error</span><span class='rparen'>)</span>
|
962
|
-
<span class='ivar'>@validation_report</span><span class='period'>.</span><span class='id identifier rubyid_errors'>errors</span><span class='period'>.</span><span class='id identifier rubyid_push'>push</span> <span class='const'>OtherError</span>
|
963
|
-
<span class='kw'>return</span> <span class='ivar'>@validation_report</span>
|
964
|
-
<span class='kw'>end</span>
|
965
|
-
<span class='kw'>end</span></pre>
|
966
|
-
</td>
|
967
|
-
</tr>
|
968
|
-
</table>
|
969
|
-
</div>
|
970
|
-
|
971
|
-
</div>
|
972
|
-
|
973
|
-
</div>
|
974
|
-
|
975
|
-
<div id="footer">
|
976
|
-
Generated on Sat Sep 28 07:01:33 2013 by
|
977
|
-
<a href="http://yardoc.org" title="Yay! A Ruby Documentation Tool" target="_parent">yard</a>
|
978
|
-
0.8.7.2 (ruby-1.9.3).
|
979
|
-
</div>
|
980
|
-
|
981
|
-
</body>
|
982
|
-
</html>
|