python-microscopy 25.11.20__cp313-cp313-win_amd64.whl

PYME/Acquire/microscope.py

@@ -0,0 +1,1050 @@
+#!/usr/bin/python
+
+##################
+# funcs.py
+#
+# Copyright David Baddeley, 2009
+# d.baddeley@auckland.ac.nz
+#
+# This program is free software: you can redistribute it and/or modify
+# it under the terms of the GNU General Public License as published by
+# the Free Software Foundation, either version 3 of the License, or
+# (at your option) any later version.
+#
+# This program is distributed in the hope that it will be useful,
+# but WITHOUT ANY WARRANTY; without even the implied warranty of
+# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.  See the
+# GNU General Public License for more details.
+#
+# You should have received a copy of the GNU General Public License
+# along with this program.  If not, see <http://www.gnu.org/licenses/>.
+#
+##################
+
+#imports for timing
+#import requests
+#import tables
+#import numpy as np
+#import yaml
+
+#import wx
+#from PYME.Acquire import previewaquisator as previewaquisator
+from PYME.Acquire.frameWrangler import FrameWrangler
+
+import PYME.Acquire.protocol as protocol
+
+from PYME.IO import MetaDataHandler
+from PYME.Acquire.Hardware import ccdCalibrator
+from PYME.Acquire.SpoolController import SpoolController
+from PYME.Acquire.ActionManager import ActionManager
+
+import sqlite3
+
+import os
+import datetime
+
+import warnings
+from PYME.contrib import dispatch
+
+#register handlers for ndarrays
+from PYME.misc import sqlitendarray
+
+import weakref
+import re
+
+import logging
+logger = logging.getLogger(__name__)
+
+from contextlib import contextmanager
+
+#class DummyJoystick(object):
+#    def enable(*args, **kwargs):
+#        pass
+
+class StateHandler(object):
+    def __init__(self, key, getFcn=None, setFcn=None, needCamRestart = False):
+        """Define a state-change handler for a give state key, based on supplied
+        get and set functions which interact with the underlying hardware.
+        
+        This wrapper class serves two functions - a) allowing the get and set methods
+        to be stored under a single dictionary key in the StateManager, and b)
+        making sure a signal is fired when the state changes.
+        
+        Parameters
+        ----------
+        
+        key : string
+            The hardware key - e.g. "Positioning.x", or "Lasers.405.Power". This
+            will also be how the hardware state is recorder in the metadata.
+        getFcn : function
+            The function to call to get the value of the parameter. Should take
+            one parameter which is the value to get
+        setFcn : function
+            The function to call to set the value of the parameter. Should take
+            one parameter which is the value to set. Not providing a setFcn results
+            in a read-only property.
+        needCamRestart : bool
+            Does this absolutely need a camera restart (e.g. we are changing which 
+            camera we are using). Will override other preferences.
+        
+        """
+        self.getValue = getFcn
+        self.setFcn = setFcn
+        self.key = key
+        
+        self.needCamRestart = needCamRestart
+        
+        self.onChange = dispatch.Signal()
+        
+    def setValue(self, value, force=False):
+        """Set the state of the controlled hardware to the given value and fire
+        a signal. The underlying function will not be called if old and new values
+        are the same unless force=True.
+        """
+        if force or (not self.getValue() == value):
+            if not self.setFcn is None:
+                self.setFcn(value)
+                self.onChange.send(self, key=self.key, value=value)
+            else:
+                logging.debug('No set method registered for key %s - assuming immutable' %  self.key)
+            
+class StateManager(object):
+    """Manages object (microscope) state by calling an appropriate state-handler
+    for a given key.
+    
+    Looks and behaves like a dictionary to the user, with individual pieces of 
+    hardware registering themselves to handle individual keys.
+    """
+    def __init__(self, scope, handlers = {}):
+        self._stateHandlers = {}
+        self._cachedStates = {}
+        
+        self._stateHandlers.update(handlers)
+        
+        self.scope = weakref.ref(scope)
+        self.stateChanged = dispatch.Signal()
+        
+    def __getitem__(self, key):
+        #print key
+        try: 
+            handler = self._stateHandlers[key]
+        except KeyError:
+            raise KeyError('No handler registered for %s' % key)
+            
+        return handler.getValue()
+        
+    def __setitem__(self, key, value):
+        self.setItem(key, value)
+        #raise NotImplementedError("Cannot set items via the dictionary interface - use setItem()instead")
+        #try: 
+        #    handler = self._stateHandlers[key]
+        #except KeyError:
+        #    raise KeyError('No handler registered for %s' % key)
+        #    
+        #return handler.setState(value)
+
+    def get(self, key, default=None):
+        try:
+            return self[key]
+        except KeyError:
+            if default is not None:
+                return default
+            else:
+                raise
+        
+    def setItem(self, key, value, stopCamera=False, force=False):
+        """ Set the value of one of our hardware components
+        
+        Parameters
+        ----------
+        
+        key : string
+            The parameter identifier - e.g. "Positioning.x"
+        value : object 
+            The value to set. This can be anything the registered handler understands
+            but designing the handlers to accept something which is easily converted
+            to text for the Metadata (see PYME.IO.MetaDataHandler) is advised.
+        stopCamera : bool
+            Should we stop the camera before setting the value and restart afterwards.
+            Useful for things like integration time  which might not be able to be 
+            changed on the fly, but also potentially as a way of achieving hardware 
+            synchronization.
+        force : bool
+            Whether we should call the set method even if the current state is 
+            already the desired state.
+        
+        """
+            
+        return self.setItems({key:value}, stopCamera, force)
+        
+    def setItems(self, stateDict, stopCamera = False, force=False, ignoreMissing=False):
+        """Set multiple items at once - see setItem for details.
+        
+        Parameters
+        ----------
+        
+        stateDict : dictionary (or dictionary like object)
+            a dictionary of key : value pairs for each piece of hardware which
+            wants to be updated.
+        stopCamera : bool
+            Should we stop the camera before setting the values and restart afterwards?
+            Useful for things like integration time  which might not be able to be 
+            changed on the fly, but also potentially as a way of achieving hardware 
+            synchronization.
+        force : bool
+            Whether we should call the set method even if the current state is 
+            already the desired state.
+        ignoreMissing : bool
+            Should we silently ignore keys that we don't have handlers for. Used
+            when setting state from e.g. sequence metadata which might have other
+            irrelevent info.
+        """
+        
+        restartCamera = False
+        
+        
+        if stopCamera:
+            try:
+                restartCamera = self.scope().frameWrangler.isRunning()
+                self.scope().frameWrangler.stop()
+            except AttributeError:
+                logger.error("We don't have a camera yet")
+            
+        
+        for key, value in stateDict.items():
+            try: 
+                handler = self._stateHandlers[key]
+                if handler.needCamRestart:
+                    #our hardware absolutely needs a camera restart - 
+                    #e.g. changing integration time on IXon. Override stopCamera
+                    #setting and force a restart.
+                    try:
+                        if self.scope().frameWrangler.isRunning():
+                           self.scope().frameWrangler.stop()
+                           restartCamera = True
+                    except AttributeError:
+                        logger.error("We don't have a camera yet")
+                       
+                handler.setValue(value, force)
+            except KeyError:
+                if not ignoreMissing:
+                    raise KeyError('No handler registered for %s' % key)
+                
+            
+        #logger.debug('sending state changed')
+        self.stateChanged.send(self)
+            
+        if restartCamera:
+            #logger.debug('preparing framewrangler')
+            self.scope().frameWrangler.Prepare()
+            logger.debug('restarting framewrangler')
+            self.scope().frameWrangler.start()
+            logger.debug('restarted framewrangler')
+        
+        #return a function which tells us if we're done (for use as a task)
+        return lambda : True
+                   
+            
+    def update(self, state):
+        """Update state from a dictionary
+        
+        Parameters
+        ----------
+        
+        state : dict
+            A dictionary containing the new state
+        """
+        return self.setItems(state)
+        
+    def __len__(self):
+        return len(self._stateHandlers)
+        
+    def keys(self):
+        return self._stateHandlers.keys()
+        
+    def __repr__(self):
+        s = 'StateManager:\n'
+        for k in self.keys():
+            s += '%s : %s\n' % (repr(k), repr(self[k]))
+            
+        return s
+        
+    def registerHandler(self, key, getFcn = None, setFcn=None, needCamRestart = False):
+        """Register a harware key and the ascociated handlers
+        
+        Parameters
+        ----------
+        
+        key : string
+            The hardware key - e.g. "Positioning.x", or "Lasers.405.Power". This
+            will also be how the hardware state is recorded in the metadata.
+        getFcn : function
+            The function to call to get the value of the parameter. Should take
+            one parameter which is the value to get
+        setFcn : function
+            The function to call to set the value of the parameter. Should take
+            one parameter which is the value to set. Not providing a setFcn results
+            in a read-only property.
+        needCamRestart : bool
+            Does this absolutely need a camera restart (e.g. we are changing which 
+            camera we are using). Will override other preferences.
+            
+        Notes
+        -----
+        There are a few conventions and special key names which should be observed
+        as this is how the camera will find hardware.
+        
+        - Keys should be hierachial, separated by dots
+        - Piezos and stages should start with "Positioning.", followed by an axis
+          name, and use um as a unit. 
+        - Lasers should start with "Laser." and define both "<lasername>.Power"
+          and "<lasername>.On" (this is to allow lasers with external 
+          switches/shutters as well as )
+        
+        """
+        self._stateHandlers[key] = StateHandler(key, getFcn, setFcn, needCamRestart)
+        
+    def registerChangeListener(self, key, callback):
+        """ Registers a function to be called when the state of a particular
+        key changes.
+        
+        The *key* and *value* are provided as keyword arguments to the callback
+        the callback should accept have the signature `callback(**kwargs)`,
+        or `callback(key, value, **kwargs)`. Other keyword arguments might also
+        be given.
+        """
+        
+        self._stateHandlers[key].onChange.connect(callback)
+            
+        
+        
+        
+    
+
+class Microscope(object):
+    """
+
+    Attributes
+    ----------
+    frameWrangler : PYME.Acquire.frameWrangler.FrameWrangler
+        Initialized in between hardware initializations and gui initializations.
+    
+    """
+    def __init__(self):
+        #list of tuples  of form (class, chan, name) describing the instaled piezo channels
+        self.piezos = []
+        self.lasers = []
+        self.hardwareChecks = []
+        
+        #entries should be of the form: "x" : (piezo, channel, multiplier)
+        # where multiplyier is what to multiply by to get the units to micrometers
+        self.positioning = {}
+        self.joystick = None
+
+        self.cam = None
+        self.cameras = {}
+        self.camControls = {}
+
+        self.stackNum = 0
+
+        #self.WantEventNotification = []
+ 
+        self.StatusCallbacks = [] #list of functions which provide status information
+        self.CleanupFunctions = [] #list of functions to be called at exit
+        self.PACallbacks = [] #list of functions to be called when a new aquisator is created
+        
+        
+        self.saturationThreshold = 16383 #14 bit
+        self.lastFrameSaturated = False
+        #self.cam.saturationIntervened = False
+        
+        self.microscope_name = None
+        
+        self.saturatedMessage = ''
+
+        protocol.scope = self
+        ccdCalibrator.setScope(self)
+        self.initDone = False
+
+        self._OpenSettingsDB()
+        
+        self.spoolController = SpoolController(self)#, defDir, **kwargs)
+        
+        self.state = StateManager(self)
+        
+        self.state.registerHandler('ActiveCamera', self.GetActiveCameraName, self._SetCamera, True)
+        self.state.registerHandler('Camera.IntegrationTime', self._GetActiveCameraIntegrationTime, self._SetActiveCameraIntegrationTime, True)
+        self.state.registerHandler('Camera.ROI', self._GetActiveCameraROI, self._SetActiveCameraROI, True)
+        self.state.registerHandler('Camera.Binning', self._GetActiveCameraBinning, self._SetActiveCameraBinning, True)
+        
+        self.actions = ActionManager(self)
+        
+        MetaDataHandler.provideStartMetadata.append(self.GenStartMetadata)
+        
+        #provision to set global metadata values in startup script
+        self.mdh = MetaDataHandler.NestedClassMDHandler()
+        
+    def EnableJoystick(self, enable=True):
+        if not self.joystick is None:
+            self.joystick.Enable(enable)   
+        
+    def GetPos(self):
+        res = {}
+        
+        axes = [k.split('.')[-1] for k in self.state.keys() if k.startswith('Positioning.')]
+        
+        for k in axes:
+            res[k] = self.state['Positioning.%s' % k]
+            
+        return res
+        
+    def SetPos(self, **kwargs):
+        state_updates = {'Positioning.%s' % k : v for k, v in kwargs.items()}
+        self.state.setItems(state_updates)
+        
+    def centre_roi_on(self, x, y):
+        """Convenience function to center the ROI on a given location"""
+        dx, dy = self.get_roi_offset()
+        
+        self.SetPos(x=(x-dx), y=(y-dy))
+
+    def get_roi_centre(self):
+        """Convenience function to get the centre of the ROI
+        
+        exists to allow both automatically and manually specified actions to use the same code
+        """
+        dx, dy = self.get_roi_offset()
+        pos = self.GetPos()
+
+        return pos['x'] + dx, pos['y'] + dy
+
+
+            
+    def GetPosRange(self):
+        #Todo - fix to use positioning
+        res = {}
+        for k in self.positioning.keys():
+            p, c, m = self.positioning[k]
+            if m > 0: 
+                res[k] = (p.GetMin(c)*m,p.GetMax(c)*m)
+            else:
+                res[k] = (p.GetMax(c)*m,p.GetMin(c)*m)
+
+        return res
+    
+    @property
+    @contextmanager
+    def settingsDB(self):
+        fstub = os.path.split(__file__)[0]
+        dbfname = os.path.join(fstub, 'PYMESettings.db')
+    
+        settingsDB = sqlite3.connect(dbfname, detect_types=sqlite3.PARSE_DECLTYPES | sqlite3.PARSE_COLNAMES)
+        #self.settingsDB.isolation_level = None
+        yield(settingsDB)
+
+        settingsDB.commit()
+        settingsDB.close()
+        
+
+    def _OpenSettingsDB(self):
+        with self.settingsDB as conn:
+            tableNames = [a[0] for a in conn.execute('SELECT name FROM sqlite_master WHERE type="table"').fetchall()]
+    
+            if not 'CCDCalibration2' in tableNames:
+                conn.execute("CREATE TABLE CCDCalibration2 (time timestamp, temperature integer, serial integer, nominalGains ndarray, trueGains ndarray)")
+            if not 'VoxelSizes' in tableNames:
+                conn.execute("CREATE TABLE VoxelSizes (ID INTEGER PRIMARY KEY, x REAL, y REAL, name TEXT)")
+            if not 'VoxelSizeHistory2' in tableNames:
+                conn.execute("CREATE TABLE VoxelSizeHistory2 (time timestamp, sizeID INTEGER, camSerial INTEGER)")
+            if not 'StartupTimes' in tableNames:
+                conn.execute("CREATE TABLE StartupTimes (component TEXT, time REAL)")
+                conn.execute("INSERT INTO StartupTimes VALUES ('total', 5)")
+
+    def GetPixelSize(self,cam=None):
+        """Get the (sample space) pixel size for the current camera
+        
+        Returns
+        -------
+        
+        pixelsize : tuple
+            the pixel size in the x and y axes, in um
+        """
+
+        if cam is None:
+            cam = self.cam
+        with self.settingsDB as conn:
+            currVoxelSizeID = conn.execute("SELECT sizeID FROM VoxelSizeHistory2 WHERE camSerial=? ORDER BY time DESC", (cam.GetSerialNumber(),)).fetchone()
+            if not currVoxelSizeID is None:
+                voxx, voxy = conn.execute("SELECT x,y FROM VoxelSizes WHERE ID=?", currVoxelSizeID).fetchone()
+                
+                return voxx*cam.GetHorizontalBin(), voxy*cam.GetVerticalBin()
+            elif hasattr(cam, 'XVals'): # change to looking for attribute so that wrapped (e.g. multiview) cameras still work
+                # read voxel size from directly from our simulated camera
+                logger.info('Reading voxel size directly from simulated camera')
+                vx_um = float(cam.XVals[1] - cam.XVals[0]) / 1.0e3
+                vy_um = float(cam.YVals[1] - cam.YVals[0]) / 1.0e3
+                return vx_um * cam.GetHorizontalBin(), vy_um * cam.GetVerticalBin()
+                    
+
+    def GenStartMetadata(self, mdh):
+        """Collects the metadata we want to record at the start of a sequence
+        
+        Parameters
+        ----------
+        
+        mdh : object derived from PYME.IO.MetaDataHandler.MDHandlerBase
+            The metadata handler to which we should write our metadata         
+        """
+        try:
+            voxx, voxy = self.GetPixelSize()
+            mdh.setEntry('voxelsize.x', voxx)
+            mdh.setEntry('voxelsize.y', voxy)
+            mdh.setEntry('voxelsize.units', 'um')
+        except TypeError:
+            logger.error('No pixel size setting available for current camera - please configure the pixel size')
+            pass
+
+        for p in self.piezos:
+            mdh.setEntry('Positioning.%s' % p[2].replace(' ', '_').replace('-', '_'), p[0].GetPos(p[1]))
+            
+        for k, v in self.GetPos().items():
+            mdh.setEntry('Positioning.%s' % k.replace(' ', '_').replace('-', '_'), v)
+        
+        try:
+            mdh['CameraOrientation.Rotate'] = self.cam.orientation['rotate']
+            mdh['CameraOrientation.FlipX'] = self.cam.orientation['flipx']
+            mdh['CameraOrientation.FlipY'] = self.cam.orientation['flipy']
+        except AttributeError:
+            pass
+        
+        if not self.microscope_name is None:
+            mdh['MicroscopeName'] = self.microscope_name
+            
+        mdh.update(self.state)
+        mdh.copyEntriesFrom(self.mdh)
+
+    def AddVoxelSizeSetting(self, name, x, y):
+        """ Adds a new voxel size setting.
+        
+        The reason for multiple settings is to easily support switching between 
+        different cameras and/or optical configurations such as tube lenses, splitters,
+        etc ...
+        
+        Parameters
+        ----------
+        
+        name : string
+            The name to use for the new setting
+        x : float
+            the pixelsize along the x axis in um
+        y : float
+            the pixelsize along the y axis in um
+        """
+        with self.settingsDB as conn:
+            conn.execute("INSERT INTO VoxelSizes (name, x, y) VALUES (?, ?, ?)", (name, x, y))
+        
+        
+
+    def SetVoxelSize(self, voxelsizename, camName=None):
+        """Set the camera voxel size, from a pre-existing voxel size seting.
+        
+        Parameters
+        ----------
+        
+        voxelsizename : string
+            The name of the voxelsize setting to use. This should have been created
+            using the **AddVoxelSize** function.
+        camName : string
+            The name of the camera to ascociate the setting with. If None, then
+            the currently selected camera is used.
+        """
+        if camName is None:
+            cam = self.cam
+        else:
+            cam = self.cameras[camName]
+
+        with self.settingsDB as conn:
+            voxelSizeID = conn.execute("SELECT ID FROM VoxelSizes WHERE name=?", (voxelsizename,)).fetchone()[0]
+            conn.execute("INSERT INTO VoxelSizeHistory2 VALUES (?, ?, ?)", (datetime.datetime.now(), voxelSizeID, cam.GetSerialNumber()))
+     
+
+
+    def satCheck(self, source, **kwargs): # check for saturation
+        """Check to see if the current frame is saturated and stop the camera/
+        close the shutter if necessary
+        
+        TODO: could use a rewrite / new inspection.        
+        """
+        import wx
+        
+        if not 'shutterOpen' in dir(self.cam):
+            return
+        im = source.currentFrame
+        IMax = im.max()
+
+        if not self.cam.shutterOpen:
+            self.cam.ADOffset = im.mean()
+        elif (IMax >= self.cam.SaturationThreshold): #is saturated
+
+            source.cam.StopAq()
+
+            if self.lastFrameSaturated: #last frame was also saturated - our intervention obviously didn't work - close the shutter
+                if 'SetShutter' in dir(source.cam):
+                    source.cam.SetShutter(False)
+                source.cam.StartExposure()
+                self.saturatedMessage = 'Camera shutter has been closed'
+                self.lastFrameSaturated = True
+                self.cam.saturationIntervened = True
+                wx.MessageBox(self.saturatedMessage, "Saturation detected", wx.OK|wx.ICON_HAND)
+                return
+
+            #fracPixelsSat = (im > self.saturationThreshold).sum().astype('f')/im.size
+
+            #try turning the e.m. gain off
+            if 'SetEMGain' in dir(source.cam) and not source.cam.GetEMGain() == 0:
+                self.oldEMGain = source.cam.GetEMGain()
+                source.cam.SetEMGain(0)
+                if self.oldEMGain  < 50: #poor chance of resolving by turning EMGain down alone
+                    if 'SetShutter' in dir(source.cam):
+                        source.cam.SetShutter(False)
+                        self.saturatedMessage = 'Camera shutter closed'
+                else:
+                    self.saturatedMessage = 'EM Gain turned down'
+                    
+                source.cam.StartExposure()
+
+                self.lastFrameSaturated = True
+                self.cam.saturationIntervened = True
+                wx.MessageBox(self.saturatedMessage, "Saturation detected", wx.OK|wx.ICON_HAND)
+                #return
+            else:
+                if 'SetShutter' in dir(source.cam):
+                    source.cam.SetShutter(False)
+                source.cam.StartExposure()
+                self.saturatedMessage = 'Camera shutter closed'
+                self.lastFrameSaturated = True
+                self.cam.saturationIntervened = True
+                wx.MessageBox(self.saturatedMessage, "Saturation detected", wx.OK|wx.ICON_HAND)
+                #return
+
+            self.lastFrameSaturated = True
+
+        else:
+            self.lastFrameSaturated = False
+
+
+    def genStatus(self):
+        """Generate a status message. TODO - move this to the GUI?"""
+        stext = ''
+        if self.cam.CamReady():
+            self.cam.GetStatus()
+            stext = 'CCD Temp: %d' % self.cam.GetCCDTemp()
+        else:
+            stext = '<Camera ERROR>'    
+            
+        if 'saturationIntervened' in dir(self.cam):
+            if self.lastFrameSaturated:
+                stext = stext + '    Camera Saturated!!'
+            if self.cam.saturationIntervened:
+                stext = stext + '    ' + self.saturatedMessage        
+                
+        #if 'step' in dir(self):
+        #    stext = stext + '   Stepper: (XPos: %1.2f  YPos: %1.2f  ZPos: %1.2f)' % (self.step.GetPosX(), self.step.GetPosY(), self.step.GetPosZ())
+
+        #stext = stext + '    Position:        
+        
+        if self.frameWrangler.isRunning():
+            try:
+                stext = stext + '    FPS = (%2.2f/%2.2f)' % (self.cam.GetFPS(),self.frameWrangler.getFPS())
+            except (AttributeError, NotImplementedError):
+                stext = stext + '    FPS = %2.2f' % self.frameWrangler.getFPS()
+
+            if 'GetNumImsBuffered' in dir(self.cam):
+                stext = stext + '    Buffer Level: %d of %d' % (self.cam.GetNumImsBuffered(), self.cam.GetBufferSize())
+        
+        for sic in self.StatusCallbacks:
+            stext = stext + '    ' + sic()
+        return stext
+
+    @property
+    def pa(self):
+        """property to catch access of what was previously called the scope.frameWrangler (the PreviewAcquisator)"""
+        warnings.warn(".pa is deprecated, please use .frameWrangler instead", DeprecationWarning)
+        return self.frameWrangler
+    
+    def startFrameWrangler(self, event_loop=None):
+        """Start the frame wrangler. Gets called during post-init phase of aquiremainframe.
+        
+        """
+        #stop an old acquisition
+        try:
+            self.frameWrangler.stop()
+        except AttributeError:
+            pass
+        
+        self.frameWrangler = FrameWrangler(self.cam, event_loop=event_loop)
+        self.frameWrangler.HardwareChecks.extend(self.hardwareChecks)
+        self.frameWrangler.Prepare()
+
+        if 'shutterOpen' in dir(self.cam):
+            #self.pa.WantFrameGroupNotification.append(self.satCheck)
+            #self.frameWrangler.onFrameGroup.connect(self.satCheck)
+            pass
+            
+        self.frameWrangler.start()
+        self.CleanupFunctions.append(self.frameWrangler.destroy)
+        
+        for cb in self.PACallbacks:
+            cb()
+            
+    ##############################
+    # The microscope object manages multiple cameras
+    #
+    # The following functions deal with selecting the active camera and
+    # performing operations on the currently active camera
+    # The integration time and ROI setting functions can be thought of as
+    # proxying the underlying calls to the camera
+
+    def _SetCamera(self, camName):
+        """Set the currently used camera by name, selecting from the dictionary
+        self.cameras. Calling code should use self.state['ActiveCamera']= camName
+        instead.
+        
+        Parameters
+        ----------
+        
+        camName : string
+            The name of the camera to switch to
+        
+        """
+        #try:
+        #    self.frameWrangler.stop()
+        #except AttributeError:
+        #    pass
+
+        #deactivate cameras
+        for c in self.cameras.values():
+            c.SetActive(False)
+            c.SetShutter(False)
+
+
+        
+        self.cam = self.cameras[camName]
+        if 'lightpath' in dir(self):
+            self.lightpath.SetPort(self.cam.port)
+        
+        self.cam.SetActive(True)
+        try:
+            self.cam.SetShutter(self.camControls[camName].cbShutter.GetValue())
+        except AttributeError:
+            pass #for cameras which don't have a shutter
+
+        # TODO this needs to move to the GUI
+        from PYME.ui import manualFoldPanel as afp
+        for k in self.cameras.keys():
+            ctrl = self.camControls[k]
+            if isinstance(ctrl, afp.foldingPane):
+                ctrl.Hide()
+            else:
+                ctrl.GetParent().Hide()  # GetParent().UnPin()
+
+        ctrl = self.camControls[camName]
+        if isinstance(ctrl, afp.foldingPane):
+            ctrl.Show()
+            ctrl.GetParent().Layout()
+        else:
+            ctrl.GetParent().Show()
+            ctrl.GetParent().GetParent().Layout()
+        # End GUI TODO
+
+        try:
+            self.frameWrangler.cam = self.cam
+            self.frameWrangler.Prepare()
+            
+            #self.frameWrangler.start()
+        except AttributeError:
+            pass
+    
+    def GetActiveCameraName(self):
+        """Get the name / key of the currently active camera"""
+        for name, cam in self.cameras.items():
+            if cam is self.cam:
+                return name
+                
+    def _SetActiveCameraIntegrationTime(self, integrationTime):
+        """
+        Sets the integration time for the active camera
+        Parameters
+        ----------
+        integrationTime: float
+            Units of seconds.
+
+
+        Notes
+        -----
+        This is a state handler, use
+        `scope.state['Camera.IntegrationTime'] = integrationTime`
+        instead of calling directly
+        """
+        self.cam.SetIntegTime(integrationTime)
+    
+    def _GetActiveCameraIntegrationTime(self):
+        """Gets the integration time for the active camera (in seconds)"""
+        return self.cam.GetIntegTime()
+        
+    def _SetActiveCameraROI(self, ROI):
+        """Sets the ROI for the active camera
+        
+        NB: This is a state handler, use 
+        `scope.state['Camera.ROI'] = ROI` 
+        instead of calling directly 
+        
+        Parameters
+        ----------
+
+        ROI : tuple / sequence
+            The co-ordinates (in pixels) of the ROI, in the form (x0, y0, x1, y1)       
+        
+        """
+        logger.debug('Setting camera ROI')
+        self.cam.SetROI(*ROI)
+        
+    def _GetActiveCameraROI(self):
+        """Gets the ROI for the active camera
+        
+        Parameters
+        ----------
+
+        ROI : tuple / sequence
+            The co-ordinates (in pixels) of the ROI, in the form (x0, y0, x1, y1)       
+        
+        """
+        
+        return self.cam.GetROI()
+
+    def _SetActiveCameraBinning(self, binning):
+        """
+        Sets Binning on the active camera
+        
+        Parameters
+        ----------
+        binning : tuple/sequence (binx, biny)
+            the binning in x and y respectively
+            
+        """
+        binx, biny = binning
+        
+        self.cam.SetHorizontalBin(binx)
+        self.cam.SetVerticalBin(biny)
+
+    def _GetActiveCameraBinning(self):
+        """
+        Sets Binning on the active camera
+
+        Parameters
+        ----------
+        binning : tuple/sequence (binx, biny)
+            the binning in x and y respectively
+
+        """
+        binx = self.cam.GetHorizontalBin()
+        biny = self.cam.GetVerticalBin()
+        
+        return (binx, biny)
+            
+    def PanCamera(self, dx, dy):
+        """Moves / pans the stage my a given offset, in pixels relative to the
+        camera, correcting for any differences in rotation and mirroring between 
+        camera and stage axes.
+        
+        TODO: fix to use state based position setting
+        """
+        vx, vy = self.GetPixelSize()
+        
+        p = self.GetPos()
+        
+        ox = p['x']
+        oy = p['y']
+        
+        if 'orientation' in dir(self.cam):
+            if self.cam.orientation['rotate']:
+                dx, dy = dy, dx
+                
+            if self.cam.orientation['flipx']:
+                dx *= -1
+                
+            if self.cam.orientation['flipy']:
+                dy *= -1
+        
+        self.SetPos(x=(ox + dx*vx), y=(oy + dy*vy))
+
+
+    def turnAllLasersOff(self):
+        """Turn all attached lasers off.
+        
+        TODO - does this fit with the new state based paradigm?
+        """
+        import re
+        for k in self.state.keys():
+            if re.match(r'Lasers\.(?P<laser_name>.*)\.On', k):
+                self.state[k] = False
+        #for l in self.lasers:
+        #    l.TurnOff()
+                
+    def initialize(self, init_script_name, locals={}):
+        from PYME.Acquire import ExecTools
+        
+        # add ExecTools functions to namespace (for backwards compatibility - note that new scripts should have
+        # `from PYME.Acquire.ExecTools import InitBG, joinBGInit, InitGUI, HWNotPresent` as their first line.
+        from PYME.Acquire.ExecTools import InitBG, joinBGInit, InitGUI, HWNotPresent
+        locals.update(InitBG=InitBG, joinBGInit=joinBGInit, InitGUI=InitGUI, HWNotPresent=HWNotPresent)
+        
+        locals.update(scope=self)
+        ExecTools.setDefaultNamespace(locals, globals())
+        self._init_thread = ExecTools.execFileBG(init_script_name, locals, globals(), then=self._post_init)
+        
+        return self._init_thread
+        
+    @property
+    def initialized(self):
+        """
+        Checks if the initialisation thread has run (replaces check of initDone flag which needed to be manually set
+        in initialisation script). Semantics are slightly different in that this returns true even if there is an error
+        whist the old way of checking would get forever stuck at the splash screen.
+        
+        Returns
+        -------
+        
+        True if the initialisation script has run, False otherwise
+
+        """
+        try:
+            return not self._init_thread.is_alive()
+        except AttributeError:
+            # don't have and _init_thread yet
+            return False
+        
+    def wait_for_init(self):
+        self._init_thread.join()
+        
+    def _post_init(self):
+        """
+        Called after the init script runs to do stuff based on what hardware is present
+        """
+        
+        if len(self.positioning) > 0:
+            # we have registered piezos => we can do z-stacks
+            from PYME.Acquire import stackSettings
+            
+            # FIXME - this looks like it will create a circular reference
+            self.stackSettings = stackSettings.StackSettings(self)
+                
+    def register_piezo(self, piezo, axis_name, multiplier=1, needCamRestart=False, channel=0):
+        """
+        Register a piezo with the microscope object
+        
+        Parameters
+        ----------
+        piezo : `PYME.Acquire.Hardware.Piezos.base_piezo.PiezoBase` instance
+            the piezo to register
+        axis_name : string
+            the axis name, e.g. 'x', 'y', 'z'
+        multiplier : float, typically either 1 or -1
+            what to multiply the positions by to match the directionality in the displayed image and make panning etc
+            work.
+        needCamRestart : bool
+            whether to restart the camera after changing the position (mostly for simulation and fake piezos)
+
+        Notes
+        -----
+        
+        The 'x' and 'y' axes should be defined in terms of the axes of the principle camera (i.e. if a stage moves the
+        image on the camera left and right it is the 'x' stage, regardless of the physical motion of the sample). Likewise,
+        the multipliers should be set so that a positive movement of the x stage moves the image right, and a positive
+        movement of the y stage moves the image down.
+
+        """
+        try:
+            display_name = piezo.gui_description % axis_name
+        except:
+            display_name = 'Piezo %s' % axis_name
+
+        self.piezos.append((piezo, channel, display_name))
+        
+        try:
+            units_um = float(piezo.units_um)
+        except:
+            units_um = 1.0
+        
+        self.positioning[axis_name] = (piezo, channel, 1*multiplier*units_um)
+        self.state.registerHandler('Positioning.%s' % axis_name, lambda: units_um*multiplier*piezo.GetPos(channel),
+                                    lambda v: piezo.MoveTo(channel, v/(multiplier*units_um)), needCamRestart=needCamRestart)
+        
+        self.state.registerHandler('Positioning.%s_target' % axis_name,
+                                       lambda: units_um*multiplier*piezo.GetTargetPos(channel)) #FIXME - target position does not belong in state ---
+        
+    def register_camera(self, cam, name, port='', rotate=False, flipx=False, flipy=False):
+        """
+        Register a camera with the microscope
+        
+        Parameters
+        ----------
+        cam     : The camera object
+        name    : A human-readable name
+        port    : Which sideport the camera is attached to (used to automatically select the right camera when changing
+                  sideports & vice versa, but currently only works on the Nikon Ti stand)
+        rotate  : Is this camera rotated with respect to the cardinal camera (used for multi-camera support)
+        flipx   : Is this camera flipped in x w.r.t. the cardinal camera (used for multi-camera support)
+        flipy   : Is this camera flipped in y w.r.t. the cardinal camera (used for multi-camera support)
+
+        Notes
+        -----
+        The optional port, rotate, flipx, and flipy parameters all relate to multiple camera support and should be
+        defined with respect to the principle camera (not stages etc which should be in the frame of the principle camera).
+        The support for these parameters is currently pretty limited - they work when panning the sample using the mouse,
+        but are generally ignored in other parts of the code.
+
+        """
+        cam.port = port
+        cam.orientation = dict(rotate=rotate, flipx=flipx, flipy=flipy)
+        
+        self.cameras[name] = cam
+        if self.cam is None:
+            self.cam = cam
+            cam.SetActive(True)
+            
+    def get_roi_offset(self):
+        """
+        Stage (positioning) coordinates are referenced to the (0,0) pixel of the primary camera. Return the offset to
+        the centre of the current ROI. Used when, e.g. moving the stage to centre the current view on a given set of
+        coordinates.
+        
+        Returns
+        -------
+        
+        roi_offset_x, roi_offset_y : float
+                                        offsets in um
+
+        """
+        from PYME.Acquire.Hardware.multiview import MultiviewWrapper
+        from PYME.Acquire.Hardware.Camera import MultiviewCameraMixin
+        
+        x0, y0, _, _ = self.state['Camera.ROI']
+    
+        if isinstance(self.cam, (MultiviewWrapper, MultiviewCameraMixin)):
+            # fix multiview crazyness
+            if self.cam.multiview_enabled:
+                #always use the 0th ROI for determining relative position, regardless of which ROIs are active
+                x0, y0 = self.cam.view_origins[0]
+        
+            roi_offset_x = self.GetPixelSize()[0] * (x0 + 0.5 * self.cam.size_x)
+            roi_offset_y = self.GetPixelSize()[1] * (y0 + 0.5 * self.cam.size_y)
+        else:
+            roi_offset_x = self.GetPixelSize()[0] * (x0 + 0.5 * self.cam.GetPicWidth())
+            roi_offset_y = self.GetPixelSize()[1] * (y0 + 0.5 * self.cam.GetPicHeight())
+            
+        return roi_offset_x, roi_offset_y
+
+    def __del__(self):
+        pass
+        #self.settingsDB.close()