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@@ -0,0 +1,163 @@
1
+ // ***************************************************************************
2
+ // bamtools.cpp (c) 2010 Derek Barnett, Erik Garrison
3
+ // Marth Lab, Department of Biology, Boston College
4
+ // ---------------------------------------------------------------------------
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+ // Last modified: 18 May 2011 (DB)
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+ // ---------------------------------------------------------------------------
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+ // Integrates a number of BamTools functionalities into a single executable.
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+ // ***************************************************************************
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+
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+ #include "bamtools_convert.h"
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+ #include "bamtools_count.h"
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+ #include "bamtools_coverage.h"
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+ #include "bamtools_filter.h"
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+ #include "bamtools_header.h"
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+ #include "bamtools_index.h"
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+ #include "bamtools_merge.h"
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+ #include "bamtools_random.h"
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+ #include "bamtools_resolve.h"
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+ #include "bamtools_revert.h"
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+ #include "bamtools_sort.h"
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+ #include "bamtools_split.h"
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+ #include "bamtools_stats.h"
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+ #include "bamtools_version.h"
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+ #include <cstdio>
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+ #include <cstdlib>
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+ #include <iostream>
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+ #include <sstream>
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+ #include <string>
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+ using namespace BamTools;
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+ using namespace std;
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+
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+ // bamtools subtool names
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+ static const string CONVERT = "convert";
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+ static const string COUNT = "count";
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+ static const string COVERAGE = "coverage";
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+ static const string FILTER = "filter";
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+ static const string HEADER = "header";
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+ static const string INDEX = "index";
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+ static const string MERGE = "merge";
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+ static const string RANDOM = "random";
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+ static const string RESOLVE = "resolve";
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+ static const string REVERT = "revert";
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+ static const string SORT = "sort";
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+ static const string SPLIT = "split";
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+ static const string STATS = "stats";
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+
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+ // bamtools help/version constants
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+ static const string HELP = "help";
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+ static const string LONG_HELP = "--help";
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+ static const string SHORT_HELP = "-h";
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+ static const string VERSION = "version";
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+ static const string LONG_VERSION = "--version";
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+ static const string SHORT_VERSION = "-v";
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+
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+ // determine if string is a help constant
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+ static bool IsHelp(char* str) {
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+ return ( str == HELP ||
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+ str == LONG_HELP ||
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+ str == SHORT_HELP );
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+ }
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+
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+ // determine if string is a version constant
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+ static bool IsVersion(char* str) {
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+ return ( str == VERSION ||
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+ str == LONG_VERSION ||
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+ str == SHORT_VERSION );
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+ }
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+
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+ // subtool factory method
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+ AbstractTool* CreateTool(const string& arg) {
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+
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+ // determine tool type based on arg
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+ if ( arg == CONVERT ) return new ConvertTool;
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+ if ( arg == COUNT ) return new CountTool;
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+ if ( arg == COVERAGE ) return new CoverageTool;
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+ if ( arg == FILTER ) return new FilterTool;
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+ if ( arg == HEADER ) return new HeaderTool;
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+ if ( arg == INDEX ) return new IndexTool;
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+ if ( arg == MERGE ) return new MergeTool;
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+ if ( arg == RANDOM ) return new RandomTool;
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+ if ( arg == RESOLVE ) return new ResolveTool;
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+ if ( arg == REVERT ) return new RevertTool;
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+ if ( arg == SORT ) return new SortTool;
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+ if ( arg == SPLIT ) return new SplitTool;
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+ if ( arg == STATS ) return new StatsTool;
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+
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+ // unknown arg
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+ return 0;
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+ }
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+
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+ // print help info
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+ int Help(int argc, char* argv[]) {
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+
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+ // check for 'bamtools help COMMAND' to print tool-specific help message
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+ if (argc > 2) {
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+
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+ // determine desired sub-tool
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+ AbstractTool* tool = CreateTool( argv[2] );
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+
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+ // if tool known, print its help screen
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+ if ( tool ) return tool->Help();
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+ }
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+
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+ // print general BamTools help message
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+ cerr << endl;
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+ cerr << "usage: bamtools [--help] COMMAND [ARGS]" << endl;
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+ cerr << endl;
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+ cerr << "Available bamtools commands:" << endl;
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+ cerr << "\tconvert Converts between BAM and a number of other formats" << endl;
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+ cerr << "\tcount Prints number of alignments in BAM file(s)" << endl;
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+ cerr << "\tcoverage Prints coverage statistics from the input BAM file" << endl;
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+ cerr << "\tfilter Filters BAM file(s) by user-specified criteria" << endl;
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+ cerr << "\theader Prints BAM header information" << endl;
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+ cerr << "\tindex Generates index for BAM file" << endl;
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+ cerr << "\tmerge Merge multiple BAM files into single file" << endl;
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+ cerr << "\trandom Select random alignments from existing BAM file(s), intended more as a testing tool." << endl;
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+ cerr << "\tresolve Resolves paired-end reads (marking the IsProperPair flag as needed)" << endl;
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+ cerr << "\trevert Removes duplicate marks and restores original base qualities" << endl;
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+ cerr << "\tsort Sorts the BAM file according to some criteria" << endl;
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+ cerr << "\tsplit Splits a BAM file on user-specified property, creating a new BAM output file for each value found" << endl;
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+ cerr << "\tstats Prints some basic statistics from input BAM file(s)" << endl;
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+ cerr << endl;
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+ cerr << "See 'bamtools help COMMAND' for more information on a specific command." << endl;
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+ cerr << endl;
125
+ return EXIT_SUCCESS;
126
+ }
127
+
128
+ // print version info
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+ int Version(void) {
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+
131
+ stringstream versionStream("");
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+ versionStream << BAMTOOLS_VERSION_MAJOR << "."
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+ << BAMTOOLS_VERSION_MINOR << "."
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+ << BAMTOOLS_VERSION_BUILD;
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+
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+ cout << endl;
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+ cout << "bamtools " << versionStream.str() << endl;
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+ cout << "Part of BamTools API and toolkit" << endl;
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+ cout << "Primary authors: Derek Barnett, Erik Garrison, Michael Stromberg" << endl;
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+ cout << "(c) 2009-2011 Marth Lab, Biology Dept., Boston College" << endl;
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+ cout << endl;
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+ return EXIT_SUCCESS;
143
+ }
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+
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+ // toolkit entry point
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+ int main(int argc, char* argv[]) {
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+
148
+ // just 'bamtools'
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+ if ( (argc == 1) ) return Help(argc, argv);
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+
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+ // 'bamtools help', 'bamtools --help', or 'bamtools -h'
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+ if ( IsHelp(argv[1]) ) return Help(argc, argv);
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+
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+ // 'bamtools version', 'bamtools --version', or 'bamtools -v'
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+ if ( IsVersion(argv[1]) ) return Version();
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+
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+ // determine desired sub-tool, run if found
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+ AbstractTool* tool = CreateTool( argv[1] );
159
+ if ( tool ) return tool->Run(argc, argv);
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+
161
+ // no tool matched, show help
162
+ return Help(argc, argv);
163
+ }