bio 0.7.0
Sign up to get free protection for your applications and to get access to all the features.
- data/bin/bioruby +107 -0
- data/bin/br_biofetch.rb +59 -0
- data/bin/br_bioflat.rb +294 -0
- data/bin/br_biogetseq.rb +57 -0
- data/bin/br_pmfetch.rb +431 -0
- data/doc/BioRuby.rd.ja +225 -0
- data/doc/Changes-0.7.rd +236 -0
- data/doc/Design.rd.ja +341 -0
- data/doc/KEGG_API.rd +1437 -0
- data/doc/KEGG_API.rd.ja +1399 -0
- data/doc/TODO.rd.ja +138 -0
- data/doc/Tutorial.rd +1138 -0
- data/doc/Tutorial.rd.ja +2110 -0
- data/etc/bioinformatics/seqdatabase.ini +210 -0
- data/lib/bio.rb +256 -0
- data/lib/bio/alignment.rb +1906 -0
- data/lib/bio/appl/bl2seq/report.rb +350 -0
- data/lib/bio/appl/blast.rb +269 -0
- data/lib/bio/appl/blast/format0.rb +1402 -0
- data/lib/bio/appl/blast/format8.rb +95 -0
- data/lib/bio/appl/blast/report.rb +652 -0
- data/lib/bio/appl/blast/rexml.rb +151 -0
- data/lib/bio/appl/blast/wublast.rb +553 -0
- data/lib/bio/appl/blast/xmlparser.rb +222 -0
- data/lib/bio/appl/blat/report.rb +392 -0
- data/lib/bio/appl/clustalw.rb +191 -0
- data/lib/bio/appl/clustalw/report.rb +154 -0
- data/lib/bio/appl/emboss.rb +68 -0
- data/lib/bio/appl/fasta.rb +262 -0
- data/lib/bio/appl/fasta/format10.rb +428 -0
- data/lib/bio/appl/fasta/format6.rb +37 -0
- data/lib/bio/appl/genscan/report.rb +570 -0
- data/lib/bio/appl/hmmer.rb +129 -0
- data/lib/bio/appl/hmmer/report.rb +556 -0
- data/lib/bio/appl/mafft.rb +222 -0
- data/lib/bio/appl/mafft/report.rb +119 -0
- data/lib/bio/appl/psort.rb +555 -0
- data/lib/bio/appl/psort/report.rb +473 -0
- data/lib/bio/appl/sim4.rb +134 -0
- data/lib/bio/appl/sim4/report.rb +501 -0
- data/lib/bio/appl/sosui/report.rb +166 -0
- data/lib/bio/appl/spidey/report.rb +604 -0
- data/lib/bio/appl/targetp/report.rb +283 -0
- data/lib/bio/appl/tmhmm/report.rb +238 -0
- data/lib/bio/command.rb +166 -0
- data/lib/bio/data/aa.rb +354 -0
- data/lib/bio/data/codontable.rb +740 -0
- data/lib/bio/data/na.rb +226 -0
- data/lib/bio/db.rb +340 -0
- data/lib/bio/db/aaindex.rb +280 -0
- data/lib/bio/db/embl/common.rb +332 -0
- data/lib/bio/db/embl/embl.rb +446 -0
- data/lib/bio/db/embl/sptr.rb +954 -0
- data/lib/bio/db/embl/swissprot.rb +32 -0
- data/lib/bio/db/embl/trembl.rb +31 -0
- data/lib/bio/db/embl/uniprot.rb +32 -0
- data/lib/bio/db/fantom.rb +604 -0
- data/lib/bio/db/fasta.rb +869 -0
- data/lib/bio/db/genbank/common.rb +299 -0
- data/lib/bio/db/genbank/ddbj.rb +34 -0
- data/lib/bio/db/genbank/genbank.rb +354 -0
- data/lib/bio/db/genbank/genpept.rb +73 -0
- data/lib/bio/db/genbank/refseq.rb +31 -0
- data/lib/bio/db/gff.rb +106 -0
- data/lib/bio/db/go.rb +497 -0
- data/lib/bio/db/kegg/brite.rb +51 -0
- data/lib/bio/db/kegg/cell.rb +88 -0
- data/lib/bio/db/kegg/compound.rb +130 -0
- data/lib/bio/db/kegg/enzyme.rb +125 -0
- data/lib/bio/db/kegg/expression.rb +173 -0
- data/lib/bio/db/kegg/genes.rb +293 -0
- data/lib/bio/db/kegg/genome.rb +362 -0
- data/lib/bio/db/kegg/glycan.rb +213 -0
- data/lib/bio/db/kegg/keggtab.rb +418 -0
- data/lib/bio/db/kegg/kgml.rb +299 -0
- data/lib/bio/db/kegg/ko.rb +178 -0
- data/lib/bio/db/kegg/reaction.rb +97 -0
- data/lib/bio/db/litdb.rb +131 -0
- data/lib/bio/db/medline.rb +317 -0
- data/lib/bio/db/nbrf.rb +199 -0
- data/lib/bio/db/pdb.rb +38 -0
- data/lib/bio/db/pdb/atom.rb +60 -0
- data/lib/bio/db/pdb/chain.rb +117 -0
- data/lib/bio/db/pdb/model.rb +106 -0
- data/lib/bio/db/pdb/pdb.rb +1682 -0
- data/lib/bio/db/pdb/residue.rb +122 -0
- data/lib/bio/db/pdb/utils.rb +234 -0
- data/lib/bio/db/prosite.rb +616 -0
- data/lib/bio/db/rebase.rb +417 -0
- data/lib/bio/db/transfac.rb +387 -0
- data/lib/bio/feature.rb +201 -0
- data/lib/bio/io/brdb.rb +103 -0
- data/lib/bio/io/das.rb +471 -0
- data/lib/bio/io/dbget.rb +212 -0
- data/lib/bio/io/ddbjxml.rb +614 -0
- data/lib/bio/io/fastacmd.rb +123 -0
- data/lib/bio/io/fetch.rb +114 -0
- data/lib/bio/io/flatfile.rb +496 -0
- data/lib/bio/io/flatfile/bdb.rb +266 -0
- data/lib/bio/io/flatfile/index.rb +1308 -0
- data/lib/bio/io/flatfile/indexer.rb +778 -0
- data/lib/bio/io/higet.rb +92 -0
- data/lib/bio/io/keggapi.rb +863 -0
- data/lib/bio/io/pubmed.rb +189 -0
- data/lib/bio/io/registry.rb +308 -0
- data/lib/bio/io/soapwsdl.rb +114 -0
- data/lib/bio/io/sql.rb +428 -0
- data/lib/bio/location.rb +650 -0
- data/lib/bio/pathway.rb +991 -0
- data/lib/bio/reference.rb +308 -0
- data/lib/bio/sequence.rb +593 -0
- data/lib/bio/shell.rb +51 -0
- data/lib/bio/shell/core.rb +512 -0
- data/lib/bio/shell/plugin/codon.rb +228 -0
- data/lib/bio/shell/plugin/entry.rb +85 -0
- data/lib/bio/shell/plugin/flatfile.rb +119 -0
- data/lib/bio/shell/plugin/keggapi.rb +187 -0
- data/lib/bio/shell/plugin/midi.rb +448 -0
- data/lib/bio/shell/plugin/obda.rb +63 -0
- data/lib/bio/shell/plugin/seq.rb +238 -0
- data/lib/bio/shell/session.rb +214 -0
- data/lib/bio/util/color_scheme.rb +214 -0
- data/lib/bio/util/color_scheme/buried.rb +78 -0
- data/lib/bio/util/color_scheme/helix.rb +78 -0
- data/lib/bio/util/color_scheme/hydropathy.rb +83 -0
- data/lib/bio/util/color_scheme/nucleotide.rb +50 -0
- data/lib/bio/util/color_scheme/strand.rb +78 -0
- data/lib/bio/util/color_scheme/taylor.rb +69 -0
- data/lib/bio/util/color_scheme/turn.rb +78 -0
- data/lib/bio/util/color_scheme/zappo.rb +69 -0
- data/lib/bio/util/contingency_table.rb +337 -0
- data/lib/bio/util/sirna.rb +306 -0
- data/lib/bioruby.rb +34 -0
- data/sample/biofetch.rb +475 -0
- data/sample/color_scheme_na.rb +99 -0
- data/sample/dbget +37 -0
- data/sample/fasta2tab.rb +99 -0
- data/sample/fsplit.rb +51 -0
- data/sample/gb2fasta.rb +31 -0
- data/sample/gb2tab.rb +325 -0
- data/sample/gbtab2mysql.rb +161 -0
- data/sample/genes2nuc.rb +33 -0
- data/sample/genes2pep.rb +33 -0
- data/sample/genes2tab.rb +81 -0
- data/sample/genome2rb.rb +29 -0
- data/sample/genome2tab.rb +76 -0
- data/sample/goslim.rb +311 -0
- data/sample/gt2fasta.rb +47 -0
- data/sample/pmfetch.rb +42 -0
- data/sample/pmsearch.rb +42 -0
- data/sample/psortplot_html.rb +222 -0
- data/sample/ssearch2tab.rb +96 -0
- data/sample/tdiary.rb +158 -0
- data/sample/tfastx2tab.rb +100 -0
- data/sample/vs-genes.rb +212 -0
- data/test/data/SOSUI/sample.report +11 -0
- data/test/data/TMHMM/sample.report +21 -0
- data/test/data/blast/eco:b0002.faa +15 -0
- data/test/data/blast/eco:b0002.faa.m0 +128 -0
- data/test/data/blast/eco:b0002.faa.m7 +65 -0
- data/test/data/blast/eco:b0002.faa.m8 +1 -0
- data/test/data/embl/AB090716.embl +65 -0
- data/test/data/genscan/sample.report +63 -0
- data/test/data/prosite/prosite.dat +2233 -0
- data/test/data/refseq/nm_126355.entret +64 -0
- data/test/data/uniprot/p53_human.uniprot +1456 -0
- data/test/runner.rb +10 -0
- data/test/unit/bio/appl/blast/test_report.rb +427 -0
- data/test/unit/bio/appl/blast/test_xmlparser.rb +400 -0
- data/test/unit/bio/appl/genscan/test_report.rb +195 -0
- data/test/unit/bio/appl/sosui/test_report.rb +94 -0
- data/test/unit/bio/appl/targetp/test_report.rb +159 -0
- data/test/unit/bio/appl/test_blast.rb +159 -0
- data/test/unit/bio/appl/test_fasta.rb +142 -0
- data/test/unit/bio/appl/tmhmm/test_report.rb +139 -0
- data/test/unit/bio/data/test_aa.rb +103 -0
- data/test/unit/bio/data/test_codontable.rb +120 -0
- data/test/unit/bio/data/test_na.rb +89 -0
- data/test/unit/bio/db/embl/test_common.rb +130 -0
- data/test/unit/bio/db/embl/test_embl.rb +227 -0
- data/test/unit/bio/db/embl/test_sptr.rb +268 -0
- data/test/unit/bio/db/embl/test_uniprot.rb +44 -0
- data/test/unit/bio/db/kegg/test_genes.rb +58 -0
- data/test/unit/bio/db/test_fasta.rb +263 -0
- data/test/unit/bio/db/test_gff.rb +140 -0
- data/test/unit/bio/db/test_prosite.rb +1450 -0
- data/test/unit/bio/io/test_ddbjxml.rb +87 -0
- data/test/unit/bio/io/test_soapwsdl.rb +45 -0
- data/test/unit/bio/shell/plugin/test_seq.rb +175 -0
- data/test/unit/bio/test_alignment.rb +1028 -0
- data/test/unit/bio/test_command.rb +71 -0
- data/test/unit/bio/test_db.rb +109 -0
- data/test/unit/bio/test_feature.rb +128 -0
- data/test/unit/bio/test_location.rb +51 -0
- data/test/unit/bio/test_pathway.rb +485 -0
- data/test/unit/bio/test_sequence.rb +386 -0
- data/test/unit/bio/test_shell.rb +31 -0
- data/test/unit/bio/util/test_color_scheme.rb +45 -0
- data/test/unit/bio/util/test_contingency_table.rb +106 -0
- data/test/unit/bio/util/test_sirna.rb +258 -0
- metadata +295 -0
@@ -0,0 +1,106 @@
|
|
1
|
+
#
|
2
|
+
# test/unit/bio/util/test_contingency_table.rb - Unit test for Bio::ContingencyTable
|
3
|
+
#
|
4
|
+
# Copyright (C) 2005 Trevor Wennblom <trevor@corevx.com>
|
5
|
+
#
|
6
|
+
# This library is free software; you can redistribute it and/or
|
7
|
+
# modify it under the terms of the GNU Lesser General Public
|
8
|
+
# License as published by the Free Software Foundation; either
|
9
|
+
# version 2 of the License, or (at your option) any later version.
|
10
|
+
#
|
11
|
+
# This library is distributed in the hope that it will be useful,
|
12
|
+
# but WITHOUT ANY WARRANTY; without even the implied warranty of
|
13
|
+
# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU
|
14
|
+
# Lesser General Public License for more details.
|
15
|
+
#
|
16
|
+
# You should have received a copy of the GNU Lesser General Public
|
17
|
+
# License along with this library; if not, write to the Free Software
|
18
|
+
# Foundation, Inc., 59 Temple Place, Suite 330, Boston, MA 02111-1307 USA
|
19
|
+
#
|
20
|
+
# $Id: test_contingency_table.rb,v 1.2 2005/11/23 11:55:17 nakao Exp $
|
21
|
+
#
|
22
|
+
|
23
|
+
require 'pathname'
|
24
|
+
libpath = Pathname.new(File.join(File.dirname(__FILE__), ['..'] * 4 , 'lib')).cleanpath.to_s
|
25
|
+
$:.unshift(libpath) unless $:.include?(libpath)
|
26
|
+
|
27
|
+
require 'test/unit'
|
28
|
+
require 'bio/util/contingency_table'
|
29
|
+
|
30
|
+
module Bio
|
31
|
+
class TestContingencyTable < Test::Unit::TestCase
|
32
|
+
|
33
|
+
def lite_example(sequences, max_length, characters)
|
34
|
+
|
35
|
+
output = []
|
36
|
+
|
37
|
+
0.upto(max_length - 1) do |i|
|
38
|
+
(i+1).upto(max_length - 1) do |j|
|
39
|
+
ctable = Bio::ContingencyTable.new( characters )
|
40
|
+
sequences.each do |seq|
|
41
|
+
i_char = seq[i].chr
|
42
|
+
j_char = seq[j].chr
|
43
|
+
ctable.table[i_char][j_char] += 1
|
44
|
+
end
|
45
|
+
chi_square = ctable.chi_square
|
46
|
+
contingency_coefficient = ctable.contingency_coefficient
|
47
|
+
output << [(i+1), (j+1), chi_square, contingency_coefficient]
|
48
|
+
end
|
49
|
+
end
|
50
|
+
|
51
|
+
return output
|
52
|
+
end
|
53
|
+
|
54
|
+
|
55
|
+
def test_lite_example
|
56
|
+
ctable = Bio::ContingencyTable
|
57
|
+
allowed_letters = 'abcdefghijk'.split('')
|
58
|
+
|
59
|
+
seqs = Array.new
|
60
|
+
seqs << 'abcde'
|
61
|
+
seqs << 'abcde'
|
62
|
+
seqs << 'kacje'
|
63
|
+
seqs << 'aacae'
|
64
|
+
seqs << 'akcfa'
|
65
|
+
seqs << 'akcfe'
|
66
|
+
|
67
|
+
length_of_every_sequence = seqs[0].size # 5 letters long
|
68
|
+
|
69
|
+
results = lite_example(seqs, length_of_every_sequence, allowed_letters)
|
70
|
+
|
71
|
+
=begin
|
72
|
+
i j chi_square contingency_coefficient
|
73
|
+
1 2 2.4 0.534522483824849
|
74
|
+
1 3 0.0 0.0
|
75
|
+
1 4 6.0 0.707106781186548
|
76
|
+
1 5 0.24 0.196116135138184
|
77
|
+
2 3 0.0 0.0
|
78
|
+
2 4 12.0 0.816496580927726
|
79
|
+
2 5 2.4 0.534522483824849
|
80
|
+
3 4 0.0 0.0
|
81
|
+
3 5 0.0 0.0
|
82
|
+
4 5 2.4 0.534522483824849
|
83
|
+
=end
|
84
|
+
|
85
|
+
|
86
|
+
#assert_equal(2.4, results[0][2])
|
87
|
+
assert_equal('2.4', results[0][2].to_s)
|
88
|
+
assert_equal('0.534522483824849', results[0][3].to_s)
|
89
|
+
|
90
|
+
assert_equal('12.0', results[5][2].to_s)
|
91
|
+
assert_equal('0.816496580927726', results[5][3].to_s)
|
92
|
+
|
93
|
+
assert_equal('2.4', results[9][2].to_s)
|
94
|
+
assert_equal('0.534522483824849', results[9][3].to_s)
|
95
|
+
|
96
|
+
ctable = Bio::ContingencyTable.new
|
97
|
+
ctable.table['a']['t'] = 4
|
98
|
+
ctable.table['a']['g'] = 2
|
99
|
+
ctable.table['g']['t'] = 3
|
100
|
+
assert_equal('1.28571428571429', ctable.chi_square.to_s)
|
101
|
+
assert_equal(ctable.column_sum_all, ctable.row_sum_all)
|
102
|
+
assert_equal(ctable.column_sum_all, ctable.table_sum_all)
|
103
|
+
end
|
104
|
+
|
105
|
+
end
|
106
|
+
end
|
@@ -0,0 +1,258 @@
|
|
1
|
+
#
|
2
|
+
# test/unit/bio/util/test_sirna.rb - Unit test for Bio::SiRNA.
|
3
|
+
#
|
4
|
+
# Copyright (C) 2005 Mitsuteru C. Nakap <n@bioruby.org>
|
5
|
+
#
|
6
|
+
# This library is free software; you can redistribute it and/or
|
7
|
+
# modify it under the terms of the GNU Lesser General Public
|
8
|
+
# License as published by the Free Software Foundation; either
|
9
|
+
# version 2 of the License, or (at your option) any later version.
|
10
|
+
#
|
11
|
+
# This library is distributed in the hope that it will be useful,
|
12
|
+
# but WITHOUT ANY WARRANTY; without even the implied warranty of
|
13
|
+
# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU
|
14
|
+
# Lesser General Public License for more details.
|
15
|
+
#
|
16
|
+
# You should have received a copy of the GNU Lesser General Public
|
17
|
+
# License along with this library; if not, write to the Free Software
|
18
|
+
# Foundation, Inc., 59 Temple Place, Suite 330, Boston, MA 02111-1307 USA
|
19
|
+
#
|
20
|
+
# $Id: test_sirna.rb,v 1.1 2005/11/14 14:46:06 nakao Exp $
|
21
|
+
#
|
22
|
+
|
23
|
+
require 'pathname'
|
24
|
+
libpath = Pathname.new(File.join(File.dirname(__FILE__), ['..'] * 4 , 'lib')).cleanpath.to_s
|
25
|
+
$:.unshift(libpath) unless $:.include?(libpath)
|
26
|
+
|
27
|
+
require 'test/unit'
|
28
|
+
require 'bio/util/sirna'
|
29
|
+
|
30
|
+
module Bio
|
31
|
+
class TestSiRNANew < Test::Unit::TestCase
|
32
|
+
def test_new
|
33
|
+
srand(1)
|
34
|
+
naseq = Bio::Sequence::NA.new("ACGT" * 100).randomize
|
35
|
+
assert(Bio::SiRNA.new(naseq))
|
36
|
+
assert(Bio::SiRNA.new(naseq, 21))
|
37
|
+
assert(Bio::SiRNA.new(naseq, 21, 60.0))
|
38
|
+
assert(Bio::SiRNA.new(naseq, 21, 60.0, 40.0))
|
39
|
+
assert_raise(ArgumentError) { Bio::SiRNA.new(naseq, 21, 60.0, 40.0, 10.0) }
|
40
|
+
end
|
41
|
+
|
42
|
+
end
|
43
|
+
|
44
|
+
class TestSiRNA < Test::Unit::TestCase
|
45
|
+
def setup
|
46
|
+
srand(1)
|
47
|
+
naseq = Bio::Sequence::NA.new("ACGT" * 100).randomize
|
48
|
+
@obj = Bio::SiRNA.new(naseq)
|
49
|
+
end
|
50
|
+
|
51
|
+
def test_antisense_size
|
52
|
+
assert_equal(21, @obj.antisense_size)
|
53
|
+
end
|
54
|
+
|
55
|
+
def test_max_gc_percent
|
56
|
+
assert_equal(60.0, @obj.max_gc_percent)
|
57
|
+
end
|
58
|
+
|
59
|
+
def test_min_gc_percent
|
60
|
+
assert_equal(40.0, @obj.min_gc_percent)
|
61
|
+
end
|
62
|
+
|
63
|
+
def test_uitei?
|
64
|
+
target = "aaGaa"
|
65
|
+
assert_equal(false, @obj.uitei?(target))
|
66
|
+
target = "aaAaa"
|
67
|
+
assert_equal(false, @obj.uitei?(target))
|
68
|
+
target = "G" * 9
|
69
|
+
assert_equal(false, @obj.uitei?(target))
|
70
|
+
end
|
71
|
+
|
72
|
+
def test_reynolds?
|
73
|
+
target = "G" * 9
|
74
|
+
assert_equal(false, @obj.reynolds?(target))
|
75
|
+
target = "aaaaAaaaaaaUaaAaaaaaAaa"
|
76
|
+
assert_equal(true, @obj.reynolds?(target))
|
77
|
+
end
|
78
|
+
|
79
|
+
def test_uitei
|
80
|
+
assert(@obj.uitei)
|
81
|
+
end
|
82
|
+
|
83
|
+
def test_reynolds
|
84
|
+
assert(@obj.reynolds)
|
85
|
+
end
|
86
|
+
|
87
|
+
def test_design
|
88
|
+
assert(@obj.design)
|
89
|
+
end
|
90
|
+
|
91
|
+
|
92
|
+
def test_design_uitei
|
93
|
+
assert(@obj.design('uitei'))
|
94
|
+
end
|
95
|
+
|
96
|
+
def test_design_reynolds
|
97
|
+
assert(@obj.design('reynolds'))
|
98
|
+
end
|
99
|
+
end
|
100
|
+
|
101
|
+
class TestSiRNAPairNew < Test::Unit::TestCase
|
102
|
+
def test_new
|
103
|
+
target = ""
|
104
|
+
sense = ""
|
105
|
+
antisense = ""
|
106
|
+
start = 0
|
107
|
+
stop = 1
|
108
|
+
rule = 'rule'
|
109
|
+
gc_percent = 60.0
|
110
|
+
assert_raise(ArgumentError) { Bio::SiRNA::Pair.new(target, sense, antisense, start, stop, rule) }
|
111
|
+
assert(Bio::SiRNA::Pair.new(target, sense, antisense, start, stop, rule, gc_percent))
|
112
|
+
assert_raise(ArgumentError) { Bio::SiRNA::Pair.new(target, sense, antisense, start, stop, rule, gc_percent, "") }
|
113
|
+
end
|
114
|
+
end
|
115
|
+
|
116
|
+
|
117
|
+
class TestSiRNAPair < Test::Unit::TestCase
|
118
|
+
def setup
|
119
|
+
srand(1)
|
120
|
+
naseq = Bio::Sequence::NA.new("ACGT" * 100).randomize
|
121
|
+
@obj = Bio::SiRNA.new(naseq).design.first
|
122
|
+
end
|
123
|
+
|
124
|
+
def test_target
|
125
|
+
assert_equal("gcggacguaaggaguauuccugu", @obj.target)
|
126
|
+
end
|
127
|
+
|
128
|
+
def test_sense
|
129
|
+
assert_equal("ggacguaaggaguauuccugu", @obj.sense)
|
130
|
+
end
|
131
|
+
|
132
|
+
def test_antisense
|
133
|
+
assert_equal("aggaauacuccuuacguccgc", @obj.antisense)
|
134
|
+
end
|
135
|
+
|
136
|
+
def test_start
|
137
|
+
assert_equal(9, @obj.start)
|
138
|
+
end
|
139
|
+
|
140
|
+
def test_stop
|
141
|
+
assert_equal(32, @obj.stop)
|
142
|
+
end
|
143
|
+
|
144
|
+
def test_rule
|
145
|
+
assert_equal("uitei", @obj.rule)
|
146
|
+
end
|
147
|
+
|
148
|
+
def test_gc_percent
|
149
|
+
assert_equal(52.0, @obj.gc_percent)
|
150
|
+
end
|
151
|
+
|
152
|
+
def test_report
|
153
|
+
report =<<END
|
154
|
+
### siRNA
|
155
|
+
Start: 9
|
156
|
+
Stop: 32
|
157
|
+
Rule: uitei
|
158
|
+
GC %: 52
|
159
|
+
Target: GCGGACGUAAGGAGUAUUCCUGU
|
160
|
+
Sense: GGACGUAAGGAGUAUUCCUGU
|
161
|
+
Antisense: CGCCUGCAUUCCUCAUAAGGA
|
162
|
+
END
|
163
|
+
assert_equal(report, @obj.report)
|
164
|
+
end
|
165
|
+
end
|
166
|
+
|
167
|
+
class TestShRNANew < Test::Unit::TestCase
|
168
|
+
def test_new
|
169
|
+
pair = ""
|
170
|
+
assert(Bio::SiRNA::ShRNA.new(pair))
|
171
|
+
assert_raise(ArgumentError) { Bio::SiRNA::ShRNA.new }
|
172
|
+
assert_raise(ArgumentError) { Bio::SiRNA::ShRNA.new(pair, "") }
|
173
|
+
end
|
174
|
+
end
|
175
|
+
|
176
|
+
class TestShRNA < Test::Unit::TestCase
|
177
|
+
def setup
|
178
|
+
srand(1)
|
179
|
+
naseq = Bio::Sequence::NA.new("ACGT" * 100).randomize
|
180
|
+
sirna = Bio::SiRNA.new(naseq)
|
181
|
+
pairs = sirna.design
|
182
|
+
@obj = Bio::SiRNA::ShRNA.new(pairs.first)
|
183
|
+
end
|
184
|
+
|
185
|
+
def test_top_strand
|
186
|
+
@obj.design
|
187
|
+
assert_equal("caccggacguaaggaguauuccugugtgtgctgtccacaggaauacuccuuacgucc", @obj.top_strand)
|
188
|
+
end
|
189
|
+
|
190
|
+
def test_top_strand_class
|
191
|
+
@obj.design
|
192
|
+
assert_equal(Bio::Sequence::NA, @obj.top_strand.class)
|
193
|
+
end
|
194
|
+
|
195
|
+
def test_top_strand_nil
|
196
|
+
assert_equal(nil, @obj.top_strand)
|
197
|
+
end
|
198
|
+
|
199
|
+
def test_bottom_strand
|
200
|
+
@obj.design
|
201
|
+
assert_equal("aaaaggacguaaggaguauuccuguggacagcacacacaggaauacuccuuacgucc", @obj.bottom_strand)
|
202
|
+
end
|
203
|
+
|
204
|
+
def test_bottom_strand_class
|
205
|
+
@obj.design
|
206
|
+
assert_equal(Bio::Sequence::NA, @obj.bottom_strand.class)
|
207
|
+
end
|
208
|
+
|
209
|
+
def test_bottom_strand_nil
|
210
|
+
assert_equal(nil, @obj.bottom_strand)
|
211
|
+
end
|
212
|
+
|
213
|
+
def test_design
|
214
|
+
assert(@obj.design)
|
215
|
+
end
|
216
|
+
|
217
|
+
def test_design_BLOCK_IT
|
218
|
+
assert_raises(NotImplementedError) { @obj.design('BLOCK-IT') }
|
219
|
+
end
|
220
|
+
|
221
|
+
def test_blocK_it
|
222
|
+
assert_equal("aaaaggacguaaggaguauuccuguggacagcacacacaggaauacuccuuacgucc", @obj.block_it)
|
223
|
+
end
|
224
|
+
|
225
|
+
def test_blocK_it_BLOCK_iT
|
226
|
+
assert_equal("aaaaggacguaaggaguauuccuguggacagcacacacaggaauacuccuuacgucc", @obj.block_it)
|
227
|
+
end
|
228
|
+
|
229
|
+
def test_blocK_it_BLOCK_IT
|
230
|
+
assert_raises(NotImplementedError) { @obj.block_it('BLOCK-IT') }
|
231
|
+
end
|
232
|
+
|
233
|
+
def test_blocK_it_piGene
|
234
|
+
assert_equal("aaaaggacguaaggaguauuccuguggacagcacacacaggaauacuccuuacgucc", @obj.block_it('piGENE'))
|
235
|
+
end
|
236
|
+
|
237
|
+
def test_blocK_it_
|
238
|
+
assert_raises(NotImplementedError) { @obj.block_it("") }
|
239
|
+
end
|
240
|
+
|
241
|
+
def test_report
|
242
|
+
report =<<END
|
243
|
+
### shRNA
|
244
|
+
Top strand shRNA (57 nt):
|
245
|
+
5'-CACCGGACGUAAGGAGUAUUCCUGUGTGTGCTGTCCACAGGAAUACUCCUUACGUCC-3'
|
246
|
+
Bottom strand shRNA (57 nt):
|
247
|
+
3'-CCUGCAUUCCUCAUAAGGACACACACGACAGGUGUCCUUAUGAGGAAUGCAGGAAAA-5'
|
248
|
+
END
|
249
|
+
@obj.design
|
250
|
+
assert_equal(report, @obj.report)
|
251
|
+
end
|
252
|
+
|
253
|
+
def test_report_before_design
|
254
|
+
assert_raises(NoMethodError) { @obj.report }
|
255
|
+
end
|
256
|
+
end
|
257
|
+
|
258
|
+
end
|
metadata
ADDED
@@ -0,0 +1,295 @@
|
|
1
|
+
--- !ruby/object:Gem::Specification
|
2
|
+
rubygems_version: 0.8.11
|
3
|
+
specification_version: 1
|
4
|
+
name: bio
|
5
|
+
version: !ruby/object:Gem::Version
|
6
|
+
version: 0.7.0
|
7
|
+
date: 2005-12-19 00:00:00 +09:00
|
8
|
+
summary: BioRuby is a library for bioinformatics (biology + information science).
|
9
|
+
require_paths:
|
10
|
+
- lib
|
11
|
+
email: staff@bioruby.org
|
12
|
+
homepage: http://bioruby.org/
|
13
|
+
rubyforge_project:
|
14
|
+
description:
|
15
|
+
autorequire: bio
|
16
|
+
default_executable:
|
17
|
+
bindir: bin
|
18
|
+
has_rdoc: false
|
19
|
+
required_ruby_version: !ruby/object:Gem::Version::Requirement
|
20
|
+
requirements:
|
21
|
+
-
|
22
|
+
- ">"
|
23
|
+
- !ruby/object:Gem::Version
|
24
|
+
version: 0.0.0
|
25
|
+
version:
|
26
|
+
platform: ruby
|
27
|
+
signing_key:
|
28
|
+
cert_chain:
|
29
|
+
authors:
|
30
|
+
- BioRuby project
|
31
|
+
files:
|
32
|
+
- bin/bioruby
|
33
|
+
- bin/br_biofetch.rb
|
34
|
+
- bin/br_bioflat.rb
|
35
|
+
- bin/br_biogetseq.rb
|
36
|
+
- bin/br_pmfetch.rb
|
37
|
+
- doc/BioRuby.rd.ja
|
38
|
+
- doc/Changes-0.7.rd
|
39
|
+
- doc/Design.rd.ja
|
40
|
+
- doc/KEGG_API.rd
|
41
|
+
- doc/KEGG_API.rd.ja
|
42
|
+
- doc/TODO.rd.ja
|
43
|
+
- doc/Tutorial.rd
|
44
|
+
- doc/Tutorial.rd.ja
|
45
|
+
- etc/bioinformatics
|
46
|
+
- etc/bioinformatics/seqdatabase.ini
|
47
|
+
- lib/bio
|
48
|
+
- lib/bio.rb
|
49
|
+
- lib/bioruby.rb
|
50
|
+
- lib/bio/alignment.rb
|
51
|
+
- lib/bio/appl
|
52
|
+
- lib/bio/command.rb
|
53
|
+
- lib/bio/data
|
54
|
+
- lib/bio/db
|
55
|
+
- lib/bio/db.rb
|
56
|
+
- lib/bio/feature.rb
|
57
|
+
- lib/bio/io
|
58
|
+
- lib/bio/location.rb
|
59
|
+
- lib/bio/pathway.rb
|
60
|
+
- lib/bio/reference.rb
|
61
|
+
- lib/bio/sequence.rb
|
62
|
+
- lib/bio/shell
|
63
|
+
- lib/bio/shell.rb
|
64
|
+
- lib/bio/util
|
65
|
+
- lib/bio/appl/bl2seq
|
66
|
+
- lib/bio/appl/blast
|
67
|
+
- lib/bio/appl/blast.rb
|
68
|
+
- lib/bio/appl/blat
|
69
|
+
- lib/bio/appl/clustalw
|
70
|
+
- lib/bio/appl/clustalw.rb
|
71
|
+
- lib/bio/appl/emboss.rb
|
72
|
+
- lib/bio/appl/fasta
|
73
|
+
- lib/bio/appl/fasta.rb
|
74
|
+
- lib/bio/appl/genscan
|
75
|
+
- lib/bio/appl/hmmer
|
76
|
+
- lib/bio/appl/hmmer.rb
|
77
|
+
- lib/bio/appl/mafft
|
78
|
+
- lib/bio/appl/mafft.rb
|
79
|
+
- lib/bio/appl/psort
|
80
|
+
- lib/bio/appl/psort.rb
|
81
|
+
- lib/bio/appl/sim4
|
82
|
+
- lib/bio/appl/sim4.rb
|
83
|
+
- lib/bio/appl/sosui
|
84
|
+
- lib/bio/appl/spidey
|
85
|
+
- lib/bio/appl/targetp
|
86
|
+
- lib/bio/appl/tmhmm
|
87
|
+
- lib/bio/appl/bl2seq/report.rb
|
88
|
+
- lib/bio/appl/blast/format0.rb
|
89
|
+
- lib/bio/appl/blast/format8.rb
|
90
|
+
- lib/bio/appl/blast/report.rb
|
91
|
+
- lib/bio/appl/blast/rexml.rb
|
92
|
+
- lib/bio/appl/blast/wublast.rb
|
93
|
+
- lib/bio/appl/blast/xmlparser.rb
|
94
|
+
- lib/bio/appl/blat/report.rb
|
95
|
+
- lib/bio/appl/clustalw/report.rb
|
96
|
+
- lib/bio/appl/fasta/format10.rb
|
97
|
+
- lib/bio/appl/fasta/format6.rb
|
98
|
+
- lib/bio/appl/genscan/report.rb
|
99
|
+
- lib/bio/appl/hmmer/report.rb
|
100
|
+
- lib/bio/appl/mafft/report.rb
|
101
|
+
- lib/bio/appl/psort/report.rb
|
102
|
+
- lib/bio/appl/sim4/report.rb
|
103
|
+
- lib/bio/appl/sosui/report.rb
|
104
|
+
- lib/bio/appl/spidey/report.rb
|
105
|
+
- lib/bio/appl/targetp/report.rb
|
106
|
+
- lib/bio/appl/tmhmm/report.rb
|
107
|
+
- lib/bio/data/aa.rb
|
108
|
+
- lib/bio/data/codontable.rb
|
109
|
+
- lib/bio/data/na.rb
|
110
|
+
- lib/bio/db/aaindex.rb
|
111
|
+
- lib/bio/db/embl
|
112
|
+
- lib/bio/db/fantom.rb
|
113
|
+
- lib/bio/db/fasta.rb
|
114
|
+
- lib/bio/db/genbank
|
115
|
+
- lib/bio/db/gff.rb
|
116
|
+
- lib/bio/db/go.rb
|
117
|
+
- lib/bio/db/kegg
|
118
|
+
- lib/bio/db/litdb.rb
|
119
|
+
- lib/bio/db/medline.rb
|
120
|
+
- lib/bio/db/nbrf.rb
|
121
|
+
- lib/bio/db/pdb
|
122
|
+
- lib/bio/db/pdb.rb
|
123
|
+
- lib/bio/db/prosite.rb
|
124
|
+
- lib/bio/db/rebase.rb
|
125
|
+
- lib/bio/db/transfac.rb
|
126
|
+
- lib/bio/db/embl/common.rb
|
127
|
+
- lib/bio/db/embl/embl.rb
|
128
|
+
- lib/bio/db/embl/sptr.rb
|
129
|
+
- lib/bio/db/embl/swissprot.rb
|
130
|
+
- lib/bio/db/embl/trembl.rb
|
131
|
+
- lib/bio/db/embl/uniprot.rb
|
132
|
+
- lib/bio/db/genbank/common.rb
|
133
|
+
- lib/bio/db/genbank/ddbj.rb
|
134
|
+
- lib/bio/db/genbank/genbank.rb
|
135
|
+
- lib/bio/db/genbank/genpept.rb
|
136
|
+
- lib/bio/db/genbank/refseq.rb
|
137
|
+
- lib/bio/db/kegg/brite.rb
|
138
|
+
- lib/bio/db/kegg/cell.rb
|
139
|
+
- lib/bio/db/kegg/compound.rb
|
140
|
+
- lib/bio/db/kegg/enzyme.rb
|
141
|
+
- lib/bio/db/kegg/expression.rb
|
142
|
+
- lib/bio/db/kegg/genes.rb
|
143
|
+
- lib/bio/db/kegg/genome.rb
|
144
|
+
- lib/bio/db/kegg/glycan.rb
|
145
|
+
- lib/bio/db/kegg/keggtab.rb
|
146
|
+
- lib/bio/db/kegg/kgml.rb
|
147
|
+
- lib/bio/db/kegg/ko.rb
|
148
|
+
- lib/bio/db/kegg/reaction.rb
|
149
|
+
- lib/bio/db/pdb/atom.rb
|
150
|
+
- lib/bio/db/pdb/chain.rb
|
151
|
+
- lib/bio/db/pdb/model.rb
|
152
|
+
- lib/bio/db/pdb/pdb.rb
|
153
|
+
- lib/bio/db/pdb/residue.rb
|
154
|
+
- lib/bio/db/pdb/utils.rb
|
155
|
+
- lib/bio/io/brdb.rb
|
156
|
+
- lib/bio/io/das.rb
|
157
|
+
- lib/bio/io/dbget.rb
|
158
|
+
- lib/bio/io/ddbjxml.rb
|
159
|
+
- lib/bio/io/fastacmd.rb
|
160
|
+
- lib/bio/io/fetch.rb
|
161
|
+
- lib/bio/io/flatfile
|
162
|
+
- lib/bio/io/flatfile.rb
|
163
|
+
- lib/bio/io/higet.rb
|
164
|
+
- lib/bio/io/keggapi.rb
|
165
|
+
- lib/bio/io/pubmed.rb
|
166
|
+
- lib/bio/io/registry.rb
|
167
|
+
- lib/bio/io/soapwsdl.rb
|
168
|
+
- lib/bio/io/sql.rb
|
169
|
+
- lib/bio/io/flatfile/bdb.rb
|
170
|
+
- lib/bio/io/flatfile/index.rb
|
171
|
+
- lib/bio/io/flatfile/indexer.rb
|
172
|
+
- lib/bio/shell/core.rb
|
173
|
+
- lib/bio/shell/plugin
|
174
|
+
- lib/bio/shell/session.rb
|
175
|
+
- lib/bio/shell/plugin/codon.rb
|
176
|
+
- lib/bio/shell/plugin/entry.rb
|
177
|
+
- lib/bio/shell/plugin/flatfile.rb
|
178
|
+
- lib/bio/shell/plugin/keggapi.rb
|
179
|
+
- lib/bio/shell/plugin/midi.rb
|
180
|
+
- lib/bio/shell/plugin/obda.rb
|
181
|
+
- lib/bio/shell/plugin/seq.rb
|
182
|
+
- lib/bio/util/color_scheme
|
183
|
+
- lib/bio/util/color_scheme.rb
|
184
|
+
- lib/bio/util/contingency_table.rb
|
185
|
+
- lib/bio/util/sirna.rb
|
186
|
+
- lib/bio/util/color_scheme/buried.rb
|
187
|
+
- lib/bio/util/color_scheme/helix.rb
|
188
|
+
- lib/bio/util/color_scheme/hydropathy.rb
|
189
|
+
- lib/bio/util/color_scheme/nucleotide.rb
|
190
|
+
- lib/bio/util/color_scheme/strand.rb
|
191
|
+
- lib/bio/util/color_scheme/taylor.rb
|
192
|
+
- lib/bio/util/color_scheme/turn.rb
|
193
|
+
- lib/bio/util/color_scheme/zappo.rb
|
194
|
+
- sample/biofetch.rb
|
195
|
+
- sample/color_scheme_na.rb
|
196
|
+
- sample/dbget
|
197
|
+
- sample/fasta2tab.rb
|
198
|
+
- sample/fsplit.rb
|
199
|
+
- sample/gb2fasta.rb
|
200
|
+
- sample/gb2tab.rb
|
201
|
+
- sample/gbtab2mysql.rb
|
202
|
+
- sample/genes2nuc.rb
|
203
|
+
- sample/genes2pep.rb
|
204
|
+
- sample/genes2tab.rb
|
205
|
+
- sample/genome2rb.rb
|
206
|
+
- sample/genome2tab.rb
|
207
|
+
- sample/goslim.rb
|
208
|
+
- sample/gt2fasta.rb
|
209
|
+
- sample/pmfetch.rb
|
210
|
+
- sample/pmsearch.rb
|
211
|
+
- sample/psortplot_html.rb
|
212
|
+
- sample/ssearch2tab.rb
|
213
|
+
- sample/tdiary.rb
|
214
|
+
- sample/tfastx2tab.rb
|
215
|
+
- sample/vs-genes.rb
|
216
|
+
- test/data
|
217
|
+
- test/functional
|
218
|
+
- test/runner.rb
|
219
|
+
- test/unit
|
220
|
+
- test/data/blast
|
221
|
+
- test/data/embl
|
222
|
+
- test/data/genscan
|
223
|
+
- test/data/prosite
|
224
|
+
- test/data/refseq
|
225
|
+
- test/data/SOSUI
|
226
|
+
- test/data/TMHMM
|
227
|
+
- test/data/uniprot
|
228
|
+
- test/data/blast/eco:b0002.faa
|
229
|
+
- test/data/blast/eco:b0002.faa.m0
|
230
|
+
- test/data/blast/eco:b0002.faa.m7
|
231
|
+
- test/data/blast/eco:b0002.faa.m8
|
232
|
+
- test/data/embl/AB090716.embl
|
233
|
+
- test/data/genscan/sample.report
|
234
|
+
- test/data/prosite/prosite.dat
|
235
|
+
- test/data/refseq/nm_126355.entret
|
236
|
+
- test/data/SOSUI/sample.report
|
237
|
+
- test/data/TMHMM/sample.report
|
238
|
+
- test/data/uniprot/p53_human.uniprot
|
239
|
+
- test/functional/bio
|
240
|
+
- test/functional/bio/io
|
241
|
+
- test/unit/bio
|
242
|
+
- test/unit/bio/appl
|
243
|
+
- test/unit/bio/data
|
244
|
+
- test/unit/bio/db
|
245
|
+
- test/unit/bio/io
|
246
|
+
- test/unit/bio/shell
|
247
|
+
- test/unit/bio/test_alignment.rb
|
248
|
+
- test/unit/bio/test_command.rb
|
249
|
+
- test/unit/bio/test_db.rb
|
250
|
+
- test/unit/bio/test_feature.rb
|
251
|
+
- test/unit/bio/test_location.rb
|
252
|
+
- test/unit/bio/test_pathway.rb
|
253
|
+
- test/unit/bio/test_sequence.rb
|
254
|
+
- test/unit/bio/test_shell.rb
|
255
|
+
- test/unit/bio/util
|
256
|
+
- test/unit/bio/appl/blast
|
257
|
+
- test/unit/bio/appl/genscan
|
258
|
+
- test/unit/bio/appl/sosui
|
259
|
+
- test/unit/bio/appl/targetp
|
260
|
+
- test/unit/bio/appl/test_blast.rb
|
261
|
+
- test/unit/bio/appl/test_fasta.rb
|
262
|
+
- test/unit/bio/appl/tmhmm
|
263
|
+
- test/unit/bio/appl/blast/test_report.rb
|
264
|
+
- test/unit/bio/appl/blast/test_xmlparser.rb
|
265
|
+
- test/unit/bio/appl/genscan/test_report.rb
|
266
|
+
- test/unit/bio/appl/sosui/test_report.rb
|
267
|
+
- test/unit/bio/appl/targetp/test_report.rb
|
268
|
+
- test/unit/bio/appl/tmhmm/test_report.rb
|
269
|
+
- test/unit/bio/data/test_aa.rb
|
270
|
+
- test/unit/bio/data/test_codontable.rb
|
271
|
+
- test/unit/bio/data/test_na.rb
|
272
|
+
- test/unit/bio/db/embl
|
273
|
+
- test/unit/bio/db/kegg
|
274
|
+
- test/unit/bio/db/test_fasta.rb
|
275
|
+
- test/unit/bio/db/test_gff.rb
|
276
|
+
- test/unit/bio/db/test_prosite.rb
|
277
|
+
- test/unit/bio/db/embl/test_common.rb
|
278
|
+
- test/unit/bio/db/embl/test_embl.rb
|
279
|
+
- test/unit/bio/db/embl/test_sptr.rb
|
280
|
+
- test/unit/bio/db/embl/test_uniprot.rb
|
281
|
+
- test/unit/bio/db/kegg/test_genes.rb
|
282
|
+
- test/unit/bio/io/test_ddbjxml.rb
|
283
|
+
- test/unit/bio/io/test_soapwsdl.rb
|
284
|
+
- test/unit/bio/shell/plugin
|
285
|
+
- test/unit/bio/shell/plugin/test_seq.rb
|
286
|
+
- test/unit/bio/util/test_color_scheme.rb
|
287
|
+
- test/unit/bio/util/test_contingency_table.rb
|
288
|
+
- test/unit/bio/util/test_sirna.rb
|
289
|
+
test_files: []
|
290
|
+
rdoc_options: []
|
291
|
+
extra_rdoc_files: []
|
292
|
+
executables: []
|
293
|
+
extensions: []
|
294
|
+
requirements: []
|
295
|
+
dependencies: []
|