bio 0.7.0
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- data/bin/bioruby +107 -0
- data/bin/br_biofetch.rb +59 -0
- data/bin/br_bioflat.rb +294 -0
- data/bin/br_biogetseq.rb +57 -0
- data/bin/br_pmfetch.rb +431 -0
- data/doc/BioRuby.rd.ja +225 -0
- data/doc/Changes-0.7.rd +236 -0
- data/doc/Design.rd.ja +341 -0
- data/doc/KEGG_API.rd +1437 -0
- data/doc/KEGG_API.rd.ja +1399 -0
- data/doc/TODO.rd.ja +138 -0
- data/doc/Tutorial.rd +1138 -0
- data/doc/Tutorial.rd.ja +2110 -0
- data/etc/bioinformatics/seqdatabase.ini +210 -0
- data/lib/bio.rb +256 -0
- data/lib/bio/alignment.rb +1906 -0
- data/lib/bio/appl/bl2seq/report.rb +350 -0
- data/lib/bio/appl/blast.rb +269 -0
- data/lib/bio/appl/blast/format0.rb +1402 -0
- data/lib/bio/appl/blast/format8.rb +95 -0
- data/lib/bio/appl/blast/report.rb +652 -0
- data/lib/bio/appl/blast/rexml.rb +151 -0
- data/lib/bio/appl/blast/wublast.rb +553 -0
- data/lib/bio/appl/blast/xmlparser.rb +222 -0
- data/lib/bio/appl/blat/report.rb +392 -0
- data/lib/bio/appl/clustalw.rb +191 -0
- data/lib/bio/appl/clustalw/report.rb +154 -0
- data/lib/bio/appl/emboss.rb +68 -0
- data/lib/bio/appl/fasta.rb +262 -0
- data/lib/bio/appl/fasta/format10.rb +428 -0
- data/lib/bio/appl/fasta/format6.rb +37 -0
- data/lib/bio/appl/genscan/report.rb +570 -0
- data/lib/bio/appl/hmmer.rb +129 -0
- data/lib/bio/appl/hmmer/report.rb +556 -0
- data/lib/bio/appl/mafft.rb +222 -0
- data/lib/bio/appl/mafft/report.rb +119 -0
- data/lib/bio/appl/psort.rb +555 -0
- data/lib/bio/appl/psort/report.rb +473 -0
- data/lib/bio/appl/sim4.rb +134 -0
- data/lib/bio/appl/sim4/report.rb +501 -0
- data/lib/bio/appl/sosui/report.rb +166 -0
- data/lib/bio/appl/spidey/report.rb +604 -0
- data/lib/bio/appl/targetp/report.rb +283 -0
- data/lib/bio/appl/tmhmm/report.rb +238 -0
- data/lib/bio/command.rb +166 -0
- data/lib/bio/data/aa.rb +354 -0
- data/lib/bio/data/codontable.rb +740 -0
- data/lib/bio/data/na.rb +226 -0
- data/lib/bio/db.rb +340 -0
- data/lib/bio/db/aaindex.rb +280 -0
- data/lib/bio/db/embl/common.rb +332 -0
- data/lib/bio/db/embl/embl.rb +446 -0
- data/lib/bio/db/embl/sptr.rb +954 -0
- data/lib/bio/db/embl/swissprot.rb +32 -0
- data/lib/bio/db/embl/trembl.rb +31 -0
- data/lib/bio/db/embl/uniprot.rb +32 -0
- data/lib/bio/db/fantom.rb +604 -0
- data/lib/bio/db/fasta.rb +869 -0
- data/lib/bio/db/genbank/common.rb +299 -0
- data/lib/bio/db/genbank/ddbj.rb +34 -0
- data/lib/bio/db/genbank/genbank.rb +354 -0
- data/lib/bio/db/genbank/genpept.rb +73 -0
- data/lib/bio/db/genbank/refseq.rb +31 -0
- data/lib/bio/db/gff.rb +106 -0
- data/lib/bio/db/go.rb +497 -0
- data/lib/bio/db/kegg/brite.rb +51 -0
- data/lib/bio/db/kegg/cell.rb +88 -0
- data/lib/bio/db/kegg/compound.rb +130 -0
- data/lib/bio/db/kegg/enzyme.rb +125 -0
- data/lib/bio/db/kegg/expression.rb +173 -0
- data/lib/bio/db/kegg/genes.rb +293 -0
- data/lib/bio/db/kegg/genome.rb +362 -0
- data/lib/bio/db/kegg/glycan.rb +213 -0
- data/lib/bio/db/kegg/keggtab.rb +418 -0
- data/lib/bio/db/kegg/kgml.rb +299 -0
- data/lib/bio/db/kegg/ko.rb +178 -0
- data/lib/bio/db/kegg/reaction.rb +97 -0
- data/lib/bio/db/litdb.rb +131 -0
- data/lib/bio/db/medline.rb +317 -0
- data/lib/bio/db/nbrf.rb +199 -0
- data/lib/bio/db/pdb.rb +38 -0
- data/lib/bio/db/pdb/atom.rb +60 -0
- data/lib/bio/db/pdb/chain.rb +117 -0
- data/lib/bio/db/pdb/model.rb +106 -0
- data/lib/bio/db/pdb/pdb.rb +1682 -0
- data/lib/bio/db/pdb/residue.rb +122 -0
- data/lib/bio/db/pdb/utils.rb +234 -0
- data/lib/bio/db/prosite.rb +616 -0
- data/lib/bio/db/rebase.rb +417 -0
- data/lib/bio/db/transfac.rb +387 -0
- data/lib/bio/feature.rb +201 -0
- data/lib/bio/io/brdb.rb +103 -0
- data/lib/bio/io/das.rb +471 -0
- data/lib/bio/io/dbget.rb +212 -0
- data/lib/bio/io/ddbjxml.rb +614 -0
- data/lib/bio/io/fastacmd.rb +123 -0
- data/lib/bio/io/fetch.rb +114 -0
- data/lib/bio/io/flatfile.rb +496 -0
- data/lib/bio/io/flatfile/bdb.rb +266 -0
- data/lib/bio/io/flatfile/index.rb +1308 -0
- data/lib/bio/io/flatfile/indexer.rb +778 -0
- data/lib/bio/io/higet.rb +92 -0
- data/lib/bio/io/keggapi.rb +863 -0
- data/lib/bio/io/pubmed.rb +189 -0
- data/lib/bio/io/registry.rb +308 -0
- data/lib/bio/io/soapwsdl.rb +114 -0
- data/lib/bio/io/sql.rb +428 -0
- data/lib/bio/location.rb +650 -0
- data/lib/bio/pathway.rb +991 -0
- data/lib/bio/reference.rb +308 -0
- data/lib/bio/sequence.rb +593 -0
- data/lib/bio/shell.rb +51 -0
- data/lib/bio/shell/core.rb +512 -0
- data/lib/bio/shell/plugin/codon.rb +228 -0
- data/lib/bio/shell/plugin/entry.rb +85 -0
- data/lib/bio/shell/plugin/flatfile.rb +119 -0
- data/lib/bio/shell/plugin/keggapi.rb +187 -0
- data/lib/bio/shell/plugin/midi.rb +448 -0
- data/lib/bio/shell/plugin/obda.rb +63 -0
- data/lib/bio/shell/plugin/seq.rb +238 -0
- data/lib/bio/shell/session.rb +214 -0
- data/lib/bio/util/color_scheme.rb +214 -0
- data/lib/bio/util/color_scheme/buried.rb +78 -0
- data/lib/bio/util/color_scheme/helix.rb +78 -0
- data/lib/bio/util/color_scheme/hydropathy.rb +83 -0
- data/lib/bio/util/color_scheme/nucleotide.rb +50 -0
- data/lib/bio/util/color_scheme/strand.rb +78 -0
- data/lib/bio/util/color_scheme/taylor.rb +69 -0
- data/lib/bio/util/color_scheme/turn.rb +78 -0
- data/lib/bio/util/color_scheme/zappo.rb +69 -0
- data/lib/bio/util/contingency_table.rb +337 -0
- data/lib/bio/util/sirna.rb +306 -0
- data/lib/bioruby.rb +34 -0
- data/sample/biofetch.rb +475 -0
- data/sample/color_scheme_na.rb +99 -0
- data/sample/dbget +37 -0
- data/sample/fasta2tab.rb +99 -0
- data/sample/fsplit.rb +51 -0
- data/sample/gb2fasta.rb +31 -0
- data/sample/gb2tab.rb +325 -0
- data/sample/gbtab2mysql.rb +161 -0
- data/sample/genes2nuc.rb +33 -0
- data/sample/genes2pep.rb +33 -0
- data/sample/genes2tab.rb +81 -0
- data/sample/genome2rb.rb +29 -0
- data/sample/genome2tab.rb +76 -0
- data/sample/goslim.rb +311 -0
- data/sample/gt2fasta.rb +47 -0
- data/sample/pmfetch.rb +42 -0
- data/sample/pmsearch.rb +42 -0
- data/sample/psortplot_html.rb +222 -0
- data/sample/ssearch2tab.rb +96 -0
- data/sample/tdiary.rb +158 -0
- data/sample/tfastx2tab.rb +100 -0
- data/sample/vs-genes.rb +212 -0
- data/test/data/SOSUI/sample.report +11 -0
- data/test/data/TMHMM/sample.report +21 -0
- data/test/data/blast/eco:b0002.faa +15 -0
- data/test/data/blast/eco:b0002.faa.m0 +128 -0
- data/test/data/blast/eco:b0002.faa.m7 +65 -0
- data/test/data/blast/eco:b0002.faa.m8 +1 -0
- data/test/data/embl/AB090716.embl +65 -0
- data/test/data/genscan/sample.report +63 -0
- data/test/data/prosite/prosite.dat +2233 -0
- data/test/data/refseq/nm_126355.entret +64 -0
- data/test/data/uniprot/p53_human.uniprot +1456 -0
- data/test/runner.rb +10 -0
- data/test/unit/bio/appl/blast/test_report.rb +427 -0
- data/test/unit/bio/appl/blast/test_xmlparser.rb +400 -0
- data/test/unit/bio/appl/genscan/test_report.rb +195 -0
- data/test/unit/bio/appl/sosui/test_report.rb +94 -0
- data/test/unit/bio/appl/targetp/test_report.rb +159 -0
- data/test/unit/bio/appl/test_blast.rb +159 -0
- data/test/unit/bio/appl/test_fasta.rb +142 -0
- data/test/unit/bio/appl/tmhmm/test_report.rb +139 -0
- data/test/unit/bio/data/test_aa.rb +103 -0
- data/test/unit/bio/data/test_codontable.rb +120 -0
- data/test/unit/bio/data/test_na.rb +89 -0
- data/test/unit/bio/db/embl/test_common.rb +130 -0
- data/test/unit/bio/db/embl/test_embl.rb +227 -0
- data/test/unit/bio/db/embl/test_sptr.rb +268 -0
- data/test/unit/bio/db/embl/test_uniprot.rb +44 -0
- data/test/unit/bio/db/kegg/test_genes.rb +58 -0
- data/test/unit/bio/db/test_fasta.rb +263 -0
- data/test/unit/bio/db/test_gff.rb +140 -0
- data/test/unit/bio/db/test_prosite.rb +1450 -0
- data/test/unit/bio/io/test_ddbjxml.rb +87 -0
- data/test/unit/bio/io/test_soapwsdl.rb +45 -0
- data/test/unit/bio/shell/plugin/test_seq.rb +175 -0
- data/test/unit/bio/test_alignment.rb +1028 -0
- data/test/unit/bio/test_command.rb +71 -0
- data/test/unit/bio/test_db.rb +109 -0
- data/test/unit/bio/test_feature.rb +128 -0
- data/test/unit/bio/test_location.rb +51 -0
- data/test/unit/bio/test_pathway.rb +485 -0
- data/test/unit/bio/test_sequence.rb +386 -0
- data/test/unit/bio/test_shell.rb +31 -0
- data/test/unit/bio/util/test_color_scheme.rb +45 -0
- data/test/unit/bio/util/test_contingency_table.rb +106 -0
- data/test/unit/bio/util/test_sirna.rb +258 -0
- metadata +295 -0
@@ -0,0 +1,99 @@
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#!/usr/bin/env ruby
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#
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# color_scheme_na.rb - A Bio::ColorScheme demo script for Nucleic Acids
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# sequences.
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#
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# Usage:
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#
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# % ruby color_scheme_na.rb > cs-seq-fna.html
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#
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# % cat seq.fna
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# >DNA_sequence
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# acgtgtgtcatgctagtcgatcgtactagtcgtagctagtca
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# % ruby color_scheme_na.rb seq.fna > colored-seq-fna.html
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#
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#
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# Copyright (C) 2005 Mitsuteru C. Nakao <n@bioruby.org>
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#
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# This program is free software; you can redistribute it and/or modify
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# it under the terms of the GNU General Public License as published by
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# the Free Software Foundation; either version 2 of the License, or
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# (at your option) any later version.
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#
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# This program is distributed in the hope that it will be useful,
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# but WITHOUT ANY WARRANTY; without even the implied warranty of
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# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
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# GNU General Public License for more details.
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#
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# $Id: color_scheme_na.rb,v 1.1 2005/10/31 07:39:13 nakao Exp $
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#
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require 'bio'
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# returns folded sequence with <br>.
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def br(i, width = 80)
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return "<br\n>" if i % width == 0
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""
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end
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# returns sequence html doc
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def display(seq, cs)
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html = '<p style="font-family: monospace">'
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postfix = '</span>'
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i = 0
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seq.each_byte do |c|
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color = cs[c.chr]
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prefix = %Q(<span style="background:\##{color};">)
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html += prefix + c.chr + postfix
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html += br(i += 1)
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end
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html + '</p>'
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end
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# returns scheme wise html doc
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def display_scheme(scheme, naseq, aaseq)
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html = ''
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cs = eval("Bio::ColorScheme::#{scheme}")
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[naseq, aaseq].each do |seq|
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html += display(seq, cs)
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end
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return ['<div>', "<h3>#{cs}</h3>", html, '</div>']
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end
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if fna = ARGV.shift
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naseq = Bio::FastaFormat.new(File.open(fna, 'r').read).naseq
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aaseq = naseq.translate
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else
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naseq = Bio::Sequence::NA.new('acgtu' * 20).randomize
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aaseq = naseq.translate
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end
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title = 'Bio::ColorScheme for DNA sequences'
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doc = ['<html>',
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'<header>', '<title>', title, '</title>', '</header>',
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'<body>', '<h1>', title, '</h1>']
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doc << ['<div>', '<h2>', 'Simple colors', '</h2>']
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['Nucleotide'].each do |scheme|
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doc << display_scheme(scheme, naseq, "")
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end
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doc << ['</div>']
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['Zappo', 'Taylor' ].each do |scheme|
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doc << display_scheme(scheme, "", aaseq)
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end
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doc << ['</div>']
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doc << ['<div>', '<h2>', 'Score colors', '</h2>']
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['Buried', 'Helix', 'Hydropathy', 'Strand', 'Turn'].each do |score|
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doc << display_scheme(score, "", aaseq)
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end
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doc << ['</div>']
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puts doc + ['</body>','</html>']
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data/sample/dbget
ADDED
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#!/usr/bin/env ruby
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#
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# dbget - DBGET client
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#
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# Interface to GenomeNet DBGET system - http://www.genome.jp/dbget/
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#
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# Copyright (C) 2001 KATAYAMA Toshiaki <k@bioruby.org>
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#
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# This program is free software; you can redistribute it and/or modify
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# it under the terms of the GNU General Public License as published by
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# the Free Software Foundation; either version 2 of the License, or
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# (at your option) any later version.
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#
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# This program is distributed in the hope that it will be useful,
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# but WITHOUT ANY WARRANTY; without even the implied warranty of
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# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
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# GNU General Public License for more details.
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#
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# You should have received a copy of the GNU General Public License
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# along with this program; if not, write to the Free Software
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# Foundation, Inc., 59 Temple Place, Suite 330, Boston, MA 02111-1307 USA
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#
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# $Id: dbget,v 1.7 2004/08/24 00:09:24 k Exp $
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#
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require "bio/io/dbget"
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# DBGET command
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com = File.basename($0) # e.g. $PATH/bget db entry
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com = ARGV.shift if com == "dbget" # e.g. $PATH/dbget bget db entry
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# DBGET query strings
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arg = ARGV.join(" ")
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# DBGET result
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print Bio::DBGET.dbget(com, arg)
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data/sample/fasta2tab.rb
ADDED
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#!/usr/bin/env ruby
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#
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# fasta2tab.rb - convert FASTA (-m 6) output into tab delimited data for MySQL
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#
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# Usage:
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#
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# % fasta2tab.rb FASTA-output-file[s] > fasta_results.tab
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# % mysql < fasta_results.sql (use sample at the end of this file)
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#
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# Format accepted:
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#
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# % fasta3[3][_t] -Q -H -m 6 query.f target.f ktup > FASTA-output-file
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#
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# Copyright (C) 2001 KATAYAMA Toshiaki <k@bioruby.org>
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#
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# This program is free software; you can redistribute it and/or modify
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# it under the terms of the GNU General Public License as published by
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# the Free Software Foundation; either version 2 of the License, or
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# (at your option) any later version.
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#
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# This program is distributed in the hope that it will be useful,
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# but WITHOUT ANY WARRANTY; without even the implied warranty of
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# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
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# GNU General Public License for more details.
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#
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# $Id: fasta2tab.rb,v 0.1 2001/06/21 08:21:58 katayama Exp $
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#
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while gets
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# query
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if /^\S+: (\d+) aa$/
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q_len = $1
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end
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# each hit
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if /^>>([^>]\S+).*\((\d+) aa\)$/
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target = $1
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t_len = $2
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# d = dummy variable
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d, d, initn, d, init1, d, opt, d, zscore, d, bits, d, evalue =
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gets.split(/\s+/)
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+
d, d, sw, ident, d, ugident, d, d, overlap, d, d, lap =
|
45
|
+
gets.split(/\s+/)
|
46
|
+
|
47
|
+
# query-hit pair
|
48
|
+
print "#{$FILENAME}\t#{q_len}\t#{target}\t#{t_len}"
|
49
|
+
|
50
|
+
# pick up values
|
51
|
+
ary = [
|
52
|
+
initn,
|
53
|
+
init1,
|
54
|
+
opt,
|
55
|
+
zscore,
|
56
|
+
bits,
|
57
|
+
evalue,
|
58
|
+
sw,
|
59
|
+
ident,
|
60
|
+
ugident,
|
61
|
+
overlap,
|
62
|
+
lap
|
63
|
+
]
|
64
|
+
|
65
|
+
# print values
|
66
|
+
for i in ary
|
67
|
+
i.tr!('^0-9.:e\-','')
|
68
|
+
print "\t#{i}"
|
69
|
+
end
|
70
|
+
|
71
|
+
print "\n"
|
72
|
+
|
73
|
+
end
|
74
|
+
end
|
75
|
+
|
76
|
+
=begin MySQL fasta_results.sql sample
|
77
|
+
|
78
|
+
CREATE DATABASE IF NOT EXISTS db_name;
|
79
|
+
CREATE TABLE IF NOT EXISTS db_name.table_name (
|
80
|
+
query varchar(25) not NULL,
|
81
|
+
q_len integer unsigned default 0,
|
82
|
+
target varchar(25) not NULL,
|
83
|
+
t_len integer unsigned default 0,
|
84
|
+
initn integer unsigned default 0,
|
85
|
+
init1 integer unsigned default 0,
|
86
|
+
opt integer unsigned default 0,
|
87
|
+
zscore float default 0.0,
|
88
|
+
bits float default 0.0,
|
89
|
+
evalue float default 0.0,
|
90
|
+
sw integer unsigned default 0,
|
91
|
+
ident float default 0.0,
|
92
|
+
ugident float default 0.0,
|
93
|
+
overlap integer unsigned default 0,
|
94
|
+
lap_at varchar(25) default NULL
|
95
|
+
);
|
96
|
+
LOAD DATA LOCAL INFILE 'fasta_results.tab' INTO TABLE db_name.table_name;
|
97
|
+
|
98
|
+
=end
|
99
|
+
|
data/sample/fsplit.rb
ADDED
@@ -0,0 +1,51 @@
|
|
1
|
+
#!/usr/bin/env ruby
|
2
|
+
#
|
3
|
+
# fsplit.rb - split FASTA file by each n entries
|
4
|
+
#
|
5
|
+
# Copyright (C) 2001 KATAYAMA Toshiaki <k@bioruby.org>
|
6
|
+
#
|
7
|
+
# This program is free software; you can redistribute it and/or modify
|
8
|
+
# it under the terms of the GNU General Public License as published by
|
9
|
+
# the Free Software Foundation; either version 2 of the License, or
|
10
|
+
# (at your option) any later version.
|
11
|
+
#
|
12
|
+
# This program is distributed in the hope that it will be useful,
|
13
|
+
# but WITHOUT ANY WARRANTY; without even the implied warranty of
|
14
|
+
# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
|
15
|
+
# GNU General Public License for more details.
|
16
|
+
#
|
17
|
+
# $Id: fsplit.rb,v 0.1 2001/06/21 08:22:29 katayama Exp $
|
18
|
+
#
|
19
|
+
|
20
|
+
if ARGV.length != 2
|
21
|
+
|
22
|
+
print <<-USAGE
|
23
|
+
fsplit.rb - split FASTA file by each n entries
|
24
|
+
|
25
|
+
Usage :
|
26
|
+
|
27
|
+
% ./fsplit.rb 2000 seq.f
|
28
|
+
|
29
|
+
This will produce seq.f.1, seq.f.2, ... with containing 2000 sequences
|
30
|
+
in each file.
|
31
|
+
|
32
|
+
USAGE
|
33
|
+
exit 1
|
34
|
+
|
35
|
+
end
|
36
|
+
|
37
|
+
count = ARGV.shift.to_i
|
38
|
+
|
39
|
+
i = -1
|
40
|
+
|
41
|
+
while gets
|
42
|
+
if /^>/
|
43
|
+
i += 1
|
44
|
+
if i % count == 0
|
45
|
+
n = i / count
|
46
|
+
out = File.new("#{$FILENAME}.#{n+1}", "w+")
|
47
|
+
end
|
48
|
+
end
|
49
|
+
out.print
|
50
|
+
end
|
51
|
+
|
data/sample/gb2fasta.rb
ADDED
@@ -0,0 +1,31 @@
|
|
1
|
+
#!/usr/bin/env ruby
|
2
|
+
#
|
3
|
+
# gb2fasta.rb - convert GenBank entry into FASTA format (nuc)
|
4
|
+
#
|
5
|
+
# Copyright (C) 2001 KATAYAMA Toshiaki <k@bioruby.org>
|
6
|
+
# Copyright (C) 2002 Yoshinori K. Okuji <o@bioruby.org>
|
7
|
+
#
|
8
|
+
# This program is free software; you can redistribute it and/or modify
|
9
|
+
# it under the terms of the GNU General Public License as published by
|
10
|
+
# the Free Software Foundation; either version 2 of the License, or
|
11
|
+
# (at your option) any later version.
|
12
|
+
#
|
13
|
+
# This program is distributed in the hope that it will be useful,
|
14
|
+
# but WITHOUT ANY WARRANTY; without even the implied warranty of
|
15
|
+
# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
|
16
|
+
# GNU General Public License for more details.
|
17
|
+
#
|
18
|
+
# $Id: gb2fasta.rb,v 0.5 2002/07/23 04:51:24 k Exp $
|
19
|
+
#
|
20
|
+
|
21
|
+
require 'bio/io/flatfile'
|
22
|
+
require 'bio/db/genbank'
|
23
|
+
|
24
|
+
include Bio
|
25
|
+
|
26
|
+
ff = FlatFile.new(GenBank, ARGF)
|
27
|
+
|
28
|
+
while gb = ff.next_entry
|
29
|
+
print gb.seq.to_fasta("gb:#{gb.entry_id} #{gb.definition}", 70)
|
30
|
+
end
|
31
|
+
|
data/sample/gb2tab.rb
ADDED
@@ -0,0 +1,325 @@
|
|
1
|
+
#!/usr/bin/env ruby
|
2
|
+
#
|
3
|
+
# gb2tab.rb - convert GenBank into tab delimited data for MySQL
|
4
|
+
#
|
5
|
+
# Usage:
|
6
|
+
#
|
7
|
+
# % gb2tab.rb gb*.seq
|
8
|
+
#
|
9
|
+
# Copyright (C) 2001 KATAYAMA Toshiaki <k@bioruby.org>
|
10
|
+
#
|
11
|
+
# This program is free software; you can redistribute it and/or modify
|
12
|
+
# it under the terms of the GNU General Public License as published by
|
13
|
+
# the Free Software Foundation; either version 2 of the License, or
|
14
|
+
# (at your option) any later version.
|
15
|
+
#
|
16
|
+
# This program is distributed in the hope that it will be useful,
|
17
|
+
# but WITHOUT ANY WARRANTY; without even the implied warranty of
|
18
|
+
# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
|
19
|
+
# GNU General Public License for more details.
|
20
|
+
#
|
21
|
+
# $Id: gb2tab.rb,v 0.11 2002/04/22 09:10:10 k Exp $
|
22
|
+
#
|
23
|
+
|
24
|
+
require 'bio'
|
25
|
+
|
26
|
+
$stderr.puts Time.now
|
27
|
+
|
28
|
+
ARGV.each do |gbkfile|
|
29
|
+
|
30
|
+
gbk = open("#{gbkfile}")
|
31
|
+
ent = open("#{gbkfile}.ent.tab", "w")
|
32
|
+
ft = open("#{gbkfile}.ft.tab", "w")
|
33
|
+
ref = open("#{gbkfile}.ref.tab", "w")
|
34
|
+
seq = open("#{gbkfile}.seq.tab", "w")
|
35
|
+
|
36
|
+
while entry = gbk.gets(Bio::GenBank::DELIMITER)
|
37
|
+
|
38
|
+
gb = Bio::GenBank.new(entry)
|
39
|
+
|
40
|
+
### MAIN BODY
|
41
|
+
|
42
|
+
ary = [
|
43
|
+
gb.entry_id,
|
44
|
+
gb.nalen,
|
45
|
+
gb.strand,
|
46
|
+
gb.natype,
|
47
|
+
gb.circular,
|
48
|
+
gb.division,
|
49
|
+
gb.date,
|
50
|
+
gb.definition,
|
51
|
+
gb.accession,
|
52
|
+
gb.versions.inspect,
|
53
|
+
gb.keywords.inspect,
|
54
|
+
gb.segment.inspect,
|
55
|
+
gb.common_name,
|
56
|
+
gb.organism,
|
57
|
+
gb.taxonomy,
|
58
|
+
gb.comment,
|
59
|
+
gb.basecount.inspect,
|
60
|
+
gb.origin,
|
61
|
+
]
|
62
|
+
|
63
|
+
ent.puts ary.join("\t")
|
64
|
+
|
65
|
+
### FEATURES
|
66
|
+
|
67
|
+
num = 0
|
68
|
+
|
69
|
+
gb.features.each do |f|
|
70
|
+
num += 1
|
71
|
+
|
72
|
+
span_min, span_max = f.locations.span
|
73
|
+
|
74
|
+
if f.qualifiers.empty?
|
75
|
+
ary = [
|
76
|
+
gb.entry_id,
|
77
|
+
num,
|
78
|
+
f.feature,
|
79
|
+
f.position,
|
80
|
+
span_min,
|
81
|
+
span_max,
|
82
|
+
'',
|
83
|
+
'',
|
84
|
+
]
|
85
|
+
ft.puts ary.join("\t")
|
86
|
+
else
|
87
|
+
f.each do |q|
|
88
|
+
ary = [
|
89
|
+
gb.entry_id,
|
90
|
+
num,
|
91
|
+
f.feature,
|
92
|
+
f.position,
|
93
|
+
span_min,
|
94
|
+
span_max,
|
95
|
+
q.qualifier,
|
96
|
+
q.value,
|
97
|
+
]
|
98
|
+
ft.puts ary.join("\t")
|
99
|
+
end
|
100
|
+
end
|
101
|
+
|
102
|
+
end
|
103
|
+
|
104
|
+
### REFERENCE
|
105
|
+
|
106
|
+
num = 0
|
107
|
+
|
108
|
+
gb.references.each do |r|
|
109
|
+
num += 1
|
110
|
+
|
111
|
+
ary = [
|
112
|
+
gb.entry_id,
|
113
|
+
num,
|
114
|
+
r.authors.inspect,
|
115
|
+
r.title,
|
116
|
+
r.journal,
|
117
|
+
r.medline,
|
118
|
+
r.pubmed,
|
119
|
+
]
|
120
|
+
|
121
|
+
ref.puts ary.join("\t")
|
122
|
+
end
|
123
|
+
|
124
|
+
### SEQUENCE
|
125
|
+
|
126
|
+
maxlen = 16 * 10 ** 6
|
127
|
+
|
128
|
+
num = 0
|
129
|
+
|
130
|
+
0.step(gb.nalen, maxlen) do |i|
|
131
|
+
num += 1
|
132
|
+
|
133
|
+
ary = [
|
134
|
+
gb.entry_id,
|
135
|
+
num,
|
136
|
+
gb.naseq[i, maxlen]
|
137
|
+
]
|
138
|
+
|
139
|
+
seq.puts ary.join("\t")
|
140
|
+
|
141
|
+
end
|
142
|
+
|
143
|
+
end
|
144
|
+
|
145
|
+
gbk.close
|
146
|
+
ent.close
|
147
|
+
ft.close
|
148
|
+
ref.close
|
149
|
+
seq.close
|
150
|
+
|
151
|
+
end
|
152
|
+
|
153
|
+
$stderr.puts Time.now
|
154
|
+
|
155
|
+
=begin
|
156
|
+
|
157
|
+
Example usage in zsh:
|
158
|
+
|
159
|
+
% gb2tab.rb *.seq
|
160
|
+
% for i in *.seq
|
161
|
+
> do
|
162
|
+
> base=`basename $i .seq`
|
163
|
+
> ruby -pe "gsub(/%HOGE%/,'$base')" gb2tab.sql | mysql
|
164
|
+
> done
|
165
|
+
|
166
|
+
gb2tab.sql:
|
167
|
+
|
168
|
+
CREATE DATABASE IF NOT EXISTS genbank;
|
169
|
+
USE genbank;
|
170
|
+
|
171
|
+
CREATE TABLE IF NOT EXISTS %HOGE% (
|
172
|
+
id varchar(16) NOT NULL PRIMARY KEY,
|
173
|
+
nalen integer,
|
174
|
+
strand varchar(5),
|
175
|
+
natype varchar(5),
|
176
|
+
circular varchar(10),
|
177
|
+
division varchar(5),
|
178
|
+
date varchar(12),
|
179
|
+
definition varchar(255),
|
180
|
+
accession varchar(30),
|
181
|
+
versions varchar(30),
|
182
|
+
keywords varchar(255),
|
183
|
+
segment varchar(255),
|
184
|
+
source varchar(255),
|
185
|
+
organism varchar(255),
|
186
|
+
taxonomy varchar(255),
|
187
|
+
comment text,
|
188
|
+
basecount varchar(255),
|
189
|
+
origin varchar(255),
|
190
|
+
KEY (nalen),
|
191
|
+
KEY (division),
|
192
|
+
KEY (accession),
|
193
|
+
KEY (organism),
|
194
|
+
KEY (taxonomy)
|
195
|
+
);
|
196
|
+
LOAD DATA LOCAL INFILE '%HOGE%.seq.ent.tab' INTO TABLE %HOGE%;
|
197
|
+
|
198
|
+
CREATE TABLE IF NOT EXISTS %HOGE%ft (
|
199
|
+
id varchar(16) NOT NULL,
|
200
|
+
num integer,
|
201
|
+
feature varchar(30),
|
202
|
+
position text,
|
203
|
+
span_min integer,
|
204
|
+
span_max integer,
|
205
|
+
qualifier varchar(30),
|
206
|
+
value text,
|
207
|
+
KEY (id),
|
208
|
+
KEY (num),
|
209
|
+
KEY (feature),
|
210
|
+
KEY (span_min),
|
211
|
+
KEY (span_max),
|
212
|
+
KEY (qualifier)
|
213
|
+
);
|
214
|
+
LOAD DATA LOCAL INFILE '%HOGE%.seq.ft.tab' INTO TABLE %HOGE%ft;
|
215
|
+
|
216
|
+
CREATE TABLE IF NOT EXISTS %HOGE%ref (
|
217
|
+
id varchar(16) NOT NULL,
|
218
|
+
num integer,
|
219
|
+
authors text,
|
220
|
+
title text,
|
221
|
+
journal text,
|
222
|
+
medline varchar(255),
|
223
|
+
pubmed varchar(255),
|
224
|
+
KEY (id),
|
225
|
+
KEY (medline),
|
226
|
+
KEY (pubmed)
|
227
|
+
);
|
228
|
+
LOAD DATA LOCAL INFILE '%HOGE%.seq.ref.tab' INTO TABLE %HOGE%ref;
|
229
|
+
|
230
|
+
CREATE TABLE IF NOT EXISTS %HOGE%seq (
|
231
|
+
id varchar(16) NOT NULL,
|
232
|
+
num integer,
|
233
|
+
naseq mediumtext,
|
234
|
+
KEY (id)
|
235
|
+
);
|
236
|
+
LOAD DATA LOCAL INFILE '%HOGE%.seq.seq.tab' INTO TABLE %HOGE%seq;
|
237
|
+
|
238
|
+
|
239
|
+
gbmerge.sql sample:
|
240
|
+
|
241
|
+
CREATE TABLE IF NOT EXISTS ent (
|
242
|
+
id varchar(16) NOT NULL PRIMARY KEY,
|
243
|
+
nalen integer,
|
244
|
+
strand varchar(5),
|
245
|
+
natype varchar(5),
|
246
|
+
circular varchar(10),
|
247
|
+
division varchar(5),
|
248
|
+
date varchar(12),
|
249
|
+
definition varchar(255),
|
250
|
+
accession varchar(30),
|
251
|
+
versions varchar(30),
|
252
|
+
keywords varchar(255),
|
253
|
+
segment varchar(255),
|
254
|
+
source varchar(255),
|
255
|
+
organism varchar(255),
|
256
|
+
taxonomy varchar(255),
|
257
|
+
comment text,
|
258
|
+
basecount varchar(255),
|
259
|
+
origin varchar(255),
|
260
|
+
KEY (nalen),
|
261
|
+
KEY (division),
|
262
|
+
KEY (accession),
|
263
|
+
KEY (organism),
|
264
|
+
KEY (taxonomy)
|
265
|
+
) TYPE=MERGE UNION=(
|
266
|
+
gbbct1,
|
267
|
+
gbbct2,
|
268
|
+
..., # list up all tables by yourself
|
269
|
+
gbvrt
|
270
|
+
);
|
271
|
+
|
272
|
+
CREATE TABLE IF NOT EXISTS ft (
|
273
|
+
id varchar(16) NOT NULL,
|
274
|
+
num integer,
|
275
|
+
feature varchar(30),
|
276
|
+
position text,
|
277
|
+
span_min integer,
|
278
|
+
span_max integer,
|
279
|
+
qualifier varchar(30),
|
280
|
+
value text,
|
281
|
+
KEY (id),
|
282
|
+
KEY (num),
|
283
|
+
KEY (feature),
|
284
|
+
KEY (span_min),
|
285
|
+
KEY (span_max),
|
286
|
+
KEY (qualifier)
|
287
|
+
) TYPE=MERGE UNION=(
|
288
|
+
gbbct1ft,
|
289
|
+
gbbct2ft,
|
290
|
+
..., # list up all ft tables by yourself
|
291
|
+
gbvrtft
|
292
|
+
);
|
293
|
+
|
294
|
+
CREATE TABLE IF NOT EXISTS ref (
|
295
|
+
id varchar(16) NOT NULL,
|
296
|
+
num integer,
|
297
|
+
authors text,
|
298
|
+
title text,
|
299
|
+
journal text,
|
300
|
+
medline varchar(255),
|
301
|
+
pubmed varchar(255),
|
302
|
+
KEY (id),
|
303
|
+
KEY (medline),
|
304
|
+
KEY (pubmed)
|
305
|
+
) TYPE=MERGE UNION=(
|
306
|
+
gbbct1ref,
|
307
|
+
gbbct2ref,
|
308
|
+
..., # list up all ref tables by yourself
|
309
|
+
gbvrtref
|
310
|
+
);
|
311
|
+
|
312
|
+
CREATE TABLE IF NOT EXISTS seq (
|
313
|
+
id varchar(16) NOT NULL,
|
314
|
+
num integer,
|
315
|
+
naseq mediumtext,
|
316
|
+
KEY (id)
|
317
|
+
) TYPE=MERGE UNION=(
|
318
|
+
gbbct1seq,
|
319
|
+
gbbct2seq,
|
320
|
+
..., # list up all seq tables by yourself
|
321
|
+
gbvrtseq
|
322
|
+
);
|
323
|
+
|
324
|
+
=end
|
325
|
+
|