bio 0.7.0

data/lib/bio/appl/sosui/report.rb

@@ -0,0 +1,166 @@
+#
+# = bio/appl/sosui/report.rb - SOSUI report class
+# 
+# Copyright::   Copyright (C) 2003 Mitsuteru C. Nakao <n@bioruby.org>
+# License::     LGPL
+#
+#  $Id: report.rb,v 1.9 2005/12/18 15:58:41 k Exp $
+#
+# == Example
+#
+# == References
+# * http://sosui.proteome.bio.tuat.ac.jp/sosui_submit.html
+#--
+#
+#  This library is free software; you can redistribute it and/or
+#  modify it under the terms of the GNU Lesser General Public
+#  License as published by the Free Software Foundation; either
+#  version 2 of the License, or (at your option) any later version.
+#
+#  This library is distributed in the hope that it will be useful,
+#  but WITHOUT ANY WARRANTY; without even the implied warranty of
+#  MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.  See the GNU
+#  Lesser General Public License for more details.
+#
+#  You should have received a copy of the GNU Lesser General Public
+#  License along with this library; if not, write to the Free Software
+#  Foundation, Inc., 59 Temple Place, Suite 330, Boston, MA 02111-1307  USA
+#
+#++
+#
+
+
+module Bio
+
+  class SOSUI
+
+    # = SOSUI output report parsing class
+    #
+    # == References
+    # * http://sosui.proteome.bio.tuat.ac.jp/sosui_submit.html
+    class Report
+
+      # Delimiter
+      DELIMITER = "\n>"
+      RS = DELIMITER
+
+      # Query entry_id
+      attr_reader :entry_id
+
+      # Returns the prediction result whether "MEMBRANE PROTEIN" or 
+      # "SOLUBLE PROTEIN".
+      attr_reader :prediction
+
+      # Transmembrane helixes ary
+      attr_reader :tmhs
+
+      # Parser for SOSUI output report.
+      def initialize(output_report)
+        entry       = output_report.split(/\n/)
+
+        @entry_id   = entry[0].strip.sub(/^>/,'')
+        @prediction = entry[1].strip
+        @tms        = 0
+        @tmhs       = []
+        parse_tmh(entry) if /MEMBRANE/ =~ @prediction
+      end
+
+      private
+
+      # Parser for TMH lines.
+      def parse_tmh(entry)
+        entry.each do |line|
+          if /NUMBER OF TM HELIX = (\d+)/ =~ line
+            @tms = $1
+          elsif /TM (\d+) +(\d+)- *(\d+) (\w+) +(\w+)/ =~ line
+            tmh  = $1.to_i
+            range = Range.new($2.to_i, $3.to_i)
+            grade = $4
+            seq   = $5
+            @tmhs.push(TMH.new(range, grade, seq))
+          end
+        end
+      end
+
+
+      # = Bio::SOSUI::Report::TMH
+      # Container class for transmembrane helix information.
+      #  
+      #  TM 1   31-  53 SECONDARY   HIRMTFLRKVYSILSLQVLLTTV
+      class TMH
+
+        # Returns aRng of transmembrane helix
+        attr_reader :range
+        
+        # Retruns ``PRIMARY'' or ``SECONDARY'' of helix.
+        attr_reader :grade
+
+        # Returns the sequence. of transmembrane helix. 
+        attr_reader :sequence
+
+        # Sets values.
+        def initialize(range, grade, sequence)
+          @range = range
+          @grade = grade
+          @sequence = sequence
+        end
+      end
+
+    end # class Report
+
+  end # class SOSUI
+
+end # module Bio
+
+
+
+if __FILE__ == $0
+
+  begin
+    require 'pp'
+    alias p pp 
+  rescue LoadError
+  end
+
+
+  sample = <<HOGE
+>HOGE1
+ MEMBRANE PROTEIN
+ NUMBER OF TM HELIX = 6
+ TM 1   12-  34 SECONDARY   LLVPILLPEKCYDQLFVQWDLLH
+ TM 2   36-  58 PRIMARY     PCLKILLSKGLGLGIVAGSLLVK
+ TM 3  102- 124 SECONDARY   SWGEALFLMLQTITICFLVMHYR
+ TM 4  126- 148 PRIMARY     QTVKGVAFLACYGLVLLVLLSPL
+ TM 5  152- 174 SECONDARY   TVVTLLQASNVPAVVVGRLLQAA
+ TM 6  214- 236 SECONDARY   AGTFVVSSLCNGLIAAQLLFYWN
+
+>HOGE2
+ SOLUBLE PROTEIN
+
+HOGE
+
+  def hoge(ent)
+    puts '==='
+    puts ent
+    puts '==='
+    sosui = Bio::SOSUI::Report.new(ent)
+    p [:entry_id, sosui.entry_id]
+    p [:prediction, sosui.prediction]
+    p [:tmhs.size, sosui.tmhs]
+    pp [:tmhs, sosui.tmh]
+  end
+
+  sample.split(/#{Bio::SOSUI::Report::DELIMITER}/).each {|ent|
+    hoge(ent)
+  }
+
+  exit if ARGV.size == 0
+
+  while ent = $<.gets(Bio::SOSUI::Report::DELIMITER)
+    hoge(ent)
+  end
+
+end
+
+
+

data/lib/bio/appl/spidey/report.rb

@@ -0,0 +1,604 @@
+#
+# = bio/appl/spidey/report.rb - SPIDEY result parser
+#
+# Copyright:: Copyright (C) 2004 GOTO Naohisa <ng@bioruby.org>
+# License::   LGPL
+#
+#--
+#  This library is free software; you can redistribute it and/or
+#  modify it under the terms of the GNU Lesser General Public
+#  License as published by the Free Software Foundation; either
+#  version 2 of the License, or (at your option) any later version.
+#
+#  This library is distributed in the hope that it will be useful,
+#  but WITHOUT ANY WARRANTY; without even the implied warranty of
+#  MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.  See the GNU
+#  Lesser General Public License for more details.
+#
+#  You should have received a copy of the GNU Lesser General Public
+#  License along with this library; if not, write to the Free Software
+#  Foundation, Inc., 59 Temple Place, Suite 330, Boston, MA 02111-1307  USA
+#++
+#
+#  $Id: report.rb,v 1.8 2005/12/18 15:58:41 k Exp $
+#
+# NCBI Spidey result parser.
+# Currently, output of default (-p 0 option) or -p 1 option are supported.
+#
+# == Notes
+#
+# The mRNA sequence is regarded as a query, and
+# the enomic sequence is regarded as a target (subject, hit).
+#
+# == References
+#
+# * Wheelan, S.J., et al., Spidey: a tool for mRNA-to-genomic alignments,
+#   Genome Research, 11, 1952--1957, 2001.
+#   http://www.genome.org/cgi/content/abstract/11/11/1952
+# * http://www.ncbi.nlm.nih.gov/spidey/
+#
+
+require 'bio'
+
+module Bio
+  class Spidey
+
+    # Spidey report parser class.
+    # Its object may contain some Bio::Spidey::Report::Hit objects.
+    class Report #< DB
+      #--
+      # File format: -p 0 (default) or -p 1 options
+      #++
+
+      # Delimiter of each entry. Bio::FlatFile uses it.
+      DELIMITER = RS = "\n--SPIDEY "
+
+      # Creates a new Bio::Spidey::Report object from String.
+      # You can use Bio::FlatFile to read a file.
+      def initialize(str)
+        str = str.sub(/\A\s+/, '')
+        str.sub!(/\n(^\-\-SPIDEY .*)/m, '')  # remove trailing entries for sure
+        @entry_overrun = $1
+        data = str.split(/\r?\n(?:\r?\n)+/)
+        d0 = data.shift.to_s.split(/\r?\n/)
+        @hit = Hit.new(data, d0)
+        @all_hits = [ @hit ]
+        if d0.empty? or /\ANo alignment found\.\s*\z/ =~ d0[-1] then
+          @hits = []
+        else
+          @hits = [ @hit ]
+        end
+      end
+      # piece of next entry. Bio::FlatFile uses it.
+      attr_reader :entry_overrun
+
+      # Returns an Array of Bio::Spidey::Report::Hit objects.
+      # Because current version of SPIDEY supports only 1 genomic sequences,
+      # the number of hits is 1 or 0.
+      attr_reader :hits
+
+      # Returns an Array of Bio::Spidey::Report::Hit objects.
+      # Unlike Bio::Spidey::Report#hits, the method returns
+      # results of all trials of pairwise alignment.
+      # This would be a Bio::Spidey specific method.
+      attr_reader :all_hits
+
+      # SeqDesc stores sequence information of query or subject.
+      class SeqDesc
+        #--
+        # description/definitions of a sequence
+        #++
+
+        # Creates a new SeqDesc object.
+        # It is designed to be called from Bio::Spidey::Report::* classes.
+        # Users shall not call it directly.
+        def initialize(seqid, seqdef, len)
+          @entry_id   = seqid
+          @definition = seqdef
+          @len        = len
+        end
+
+        # Identifier of the sequence.
+        attr_reader :entry_id
+
+        # Definition of the sequence.
+        attr_reader :definition
+
+        # Length of the sequence.
+        attr_reader :len
+
+        # Parses piece of Spidey result text and creates a new SeqDesc object.
+        # It is designed to be called from Bio::Spidey::Report::* classes.
+        # Users shall not call it directly.
+        def self.parse(str)
+          /^(Genomic|mRNA)\:\s*(([^\s]*) (.+))\, (\d+) bp\s*$/ =~ str.to_s
+          seqid  = $3
+          seqdef = $2
+          len    = ($5 ? $5.to_i : nil)
+          self.new(seqid, seqdef, len)
+        end
+      end #class SeqDesc
+
+      # Sequence segment pair of Spidey result.
+      # Similar to Bio::Blast::Report::Hsp but lacks many methods.
+      # For mRNA-genome mapping programs, unlike other homology search
+      # programs, the class is used not only for exons but also for introns.
+      # (Note that intron data would not be available according to run-time
+      # options of the program.)
+      class SegmentPair
+        #--
+        # segment pair (like Bio::BLAST::*::Report::Hsp)
+        #++
+
+        # Creates a new SegmentPair object.
+        # It is designed to be called from Bio::Spidey::Report::* classes.
+        # Users shall not call it directly.
+        def initialize(genomic, mrna, midline, aaseqline,
+                       percent_identity, mismatches, gaps, splice_site,
+                       align_len)
+          @genomic   = genomic
+          @mrna      = mrna
+          @midline   = midline
+          @aaseqline = aaseqline
+          @percent_identity = percent_identity
+          @mismaches        = mismatches
+          @gaps             = gaps
+          @splice_site      = splice_site
+          @align_len        = align_len
+        end
+
+        # Returns segment informations of the 'Genomic'.
+        # Returns a Bio::Spidey::Report::Segment object.
+        # This would be a Bio::Spidey specific method.
+        attr_reader :genomic
+
+        # Returns segment informations of the 'mRNA'.
+        # Returns a Bio::Spidey::Report::Segment object.
+        # This would be a Bio::Spidey specific method.
+        attr_reader :mrna
+
+        # Returns the middle line of the alignment of the segment pair.
+        # Returns nil if no alignment data are available.
+        attr_reader :midline
+
+        # Returns amino acide sequence in alignment.
+        # Returns String, because white spaces is also important.
+        # Returns nil if no alignment data are available.
+        attr_reader :aaseqline
+
+        # Returns percent identity of the segment pair.
+        attr_reader :percent_identity
+
+        # Returns mismatches.
+        attr_reader :mismatches
+        alias mismatch_count mismatches
+
+        # Returns gaps.
+        attr_reader :gaps
+
+        # Returns splice site information.
+        # Returns a hash which contains :d and :a for keys and
+        # 0, 1, or nil for values.
+        # This would be a Bio::Spidey specific methods.
+        attr_reader :splice_site
+
+        # Returns alignment length of the segment pair.
+        # Returns nil if no alignment data are available.
+        attr_reader :align_len
+
+        # Creates a new SegmentPair object when the segment pair is an intron.
+        # It is designed to be called internally from
+        # Bio::Spidey::Report::* classes.
+        # Users shall not call it directly.
+        def self.new_intron(from, to, strand, aln)
+          genomic   = Segment.new(from, to, strand, aln[0])
+          mrna      = Segment.new(nil, nil, nil,    aln[2])
+          midline   = aln[1]
+          aaseqline = aln[3]
+          self.new(genomic, mrna, midline, aaseqline,
+                   nil, nil, nil, nil, nil)
+        end
+
+        # Parses a piece of Spidey result text and creates a new
+        # SegmentPair object.
+        # It is designed to be called internally from
+        # Bio::Spidey::Report::* classes.
+        # Users shall not call it directly.
+        def self.parse(str, strand, complement, aln)
+          /\AExon\s*\d+(\(\-\))?\:\s*(\d+)\-(\d+)\s*\(gen\)\s+(\d+)\-(\d+)\s*\(mRNA\)\s+id\s*([\d\.]+)\s*\%\s+mismatches\s+(\d+)\s+gaps\s+(\d+)\s+splice site\s*\(d +a\)\s*\:\s*(\d+)\s+(\d+)/ =~ str
+          if strand == 'minus' then
+            genomic = Segment.new($3, $2, strand, aln[0])
+          else
+            genomic = Segment.new($2, $3, 'plus', aln[0])
+          end
+          if complement then
+            mrna    = Segment.new($4, $5, 'minus', aln[2])
+          else
+            mrna    = Segment.new($4, $5, 'plus',  aln[2])
+          end
+          percent_identity = $6
+          mismatches = ($7 ? $7.to_i : nil)
+          gaps = ($8 ? $8.to_i : nil)
+          splice_site = {
+            :d => ($9  ? $9.to_i  : nil),
+            :a => ($10 ? $10.to_i : nil)
+          }
+          midline   = aln[1]
+          aaseqline = aln[3]
+          self.new(genomic, mrna, midline, aaseqline,
+                   percent_identity, mismatches, gaps, splice_site,
+                   (midline ? midline.length : nil))
+        end
+
+        #--
+        # Bio::BLAST::*::Report::Hsp compatible methods
+        #   Methods already defined: midline, percent_identity, 
+        #     gaps, align_len, mismatch_count
+        #++
+
+        # Returns start position of the mRNA (query) (the first position is 1).
+        def query_from;   @mrna.from;       end
+
+        # Returns end position (including its position) of the mRNA (query).
+        def query_to;     @mrna.to;         end
+
+        # Returns the sequence (with gaps) of the mRNA (query).
+        def qseq;         @mrna.seq;        end
+
+        # Returns strand information of the mRNA (query).
+        # Returns 'plus', 'minus', or nil.
+        def query_strand; @mrna.strand;     end
+
+        # Returns start position of the genomic (target, hit)
+        # (the first position is 1).
+        def hit_from;     @genomic.from;    end
+
+        # Returns end position (including its position) of the
+        # genomic (target, hit).
+        def hit_to;       @genomic.to;      end
+
+        # Returns the sequence (with gaps) of the genomic (target, hit).
+        def hseq;         @genomic.seq;     end
+
+        # Returns strand information of the genomic (target, hit).
+        # Returns 'plus', 'minus', or nil.
+        def hit_strand;   @genomic.strand;  end
+      end #class SegmentPair
+
+      # Segment informations of a segment pair.
+      class Segment
+        # Creates a new Segment object.
+        # It is designed to be called internally from
+        # Bio::Spidey::Report::* classes.
+        # Users shall not call it directly.
+        def initialize(pos_st, pos_ed, strand = nil, seq = nil)
+          @from   = pos_st ? pos_st.to_i : nil
+          @to     = pos_ed ? pos_ed.to_i : nil
+          @strand = strand
+          @seq    = seq
+        end
+
+        # start position
+        attr_reader :from
+
+        # end position
+        attr_reader :to
+
+        # strand information
+        attr_reader :strand
+
+        # sequence data
+        attr_reader :seq
+      end #class Segment
+
+      # Hit object of Spidey result.
+      # Similar to Bio::Blast::Report::Hit but lacks many methods.
+      class Hit
+        # Creates a new Hit object.
+        # It is designed to be called internally from
+        # Bio::Spidey::Report::* classes.
+        # Users shall not call it directly.
+        def initialize(data, d0)
+          @data = data
+          @d0 = d0
+        end
+
+        # Fetches fields.
+        def field_fetch(t, ary)
+          reg = Regexp.new(/^#{Regexp.escape(t)}\:\s*(.+)\s*$/)
+          if ary.find { |x| reg =~ x }
+            $1.strip
+          else
+            nil
+          end
+        end
+        private :field_fetch
+
+        # Parses information about strand.
+        def parse_strand
+          x = field_fetch('Strand', @d0)
+          if x =~ /^(.+)Reverse +complement\s*$/ then
+            @strand = $1.strip
+            @complement = true
+          else
+            @strand = x
+            @complement = nil
+          end
+        end
+        private :parse_strand
+
+        # Returns strand information of the hit.
+        # Returns 'plus', 'minus', or nil.
+        # This would be a Bio::Spidey specific method.
+        def strand
+          unless defined?(@strand); parse_strand; end
+          @strand
+        end
+
+        # Returns true if the result reports 'Reverse complement'.
+        # Otherwise, return false or nil.
+        # This would be a Bio::Spidey specific method.
+        def complement?
+          unless defined?(@complement); parse_strand; end
+          @complement
+        end
+
+        # Returns number of exons in the hit.
+        def number_of_exons
+          unless defined?(@number_of_exons)
+            @number_of_exons = field_fetch('Number of exons', @d0).to_i
+          end
+          @number_of_exons
+        end
+
+        # Returns number of splice sites of the hit.
+        def number_of_splice_sites
+          unless defined?(@number_of_splice_sites)
+            @number_of_splice_sites = 
+              field_fetch('Number of splice sites', @d0).to_i
+          end
+          @number_of_splice_sites
+        end
+
+        #  Returns overall percent identity of the hit.
+        def percent_identity
+          unless defined?(@percent_identity)
+            x = field_fetch('overall percent identity', @d0)
+            @percent_identity = 
+              (/([\d\.]+)\s*\%/ =~ x.to_s) ? $1 : nil
+          end
+          @percent_identity
+        end
+
+        # Returns missing mRNA ends of the hit.
+        def missing_mrna_ends
+          unless defined?(@missing_mrna_ends)
+            @missing_mrna_ends = field_fetch('Missing mRNA ends', @d0)
+          end
+          @missing_mrna_ends
+        end
+
+        # Returns sequence informations of the 'Genomic'.
+        # Returns a Bio::Spidey::Report::SeqDesc object.
+        # This would be a Bio::Spidey specific method.
+        def genomic
+          unless defined?(@genomic)
+            @genomic = SeqDesc.parse(@d0.find { |x| /^Genomic\:/ =~ x })
+          end
+          @genomic
+        end
+        
+        # Returns sequence informations of the mRNA.
+        # Returns a Bio::Spidey::Report::SeqDesc object.
+        # This would be a Bio::Spidey specific method.
+        def mrna
+          unless defined?(@mrna)
+            @mrna = SeqDesc.parse(@d0.find { |x| /^mRNA\:/ =~ x })
+          end
+          @mrna
+        end
+
+        # Parses segment pairs.
+        def parse_segmentpairs
+          aln = self.align.dup
+          ex = []
+          itr = []
+          segpairs = []
+          cflag  = self.complement?
+          strand = self.strand
+          if strand == 'minus' then
+            d_to = 1;  d_from = -1
+          else
+            d_to = -1; d_from = 1
+          end
+          @d0.each do |x|
+            #p x
+            if x =~ /^Exon\s*\d+(\(.*\))?\:/ then
+              if a = aln.shift then
+                y = SegmentPair.parse(x, strand, cflag, a[1])
+                ex << y
+                if a[0][0].to_s.length > 0 then
+                  to = y.genomic.from + d_to
+                  i0 = SegmentPair.new_intron(nil, to, strand, a[0])
+                  itr << i0
+                  segpairs << i0
+                end
+                segpairs << y
+                if a[2][0].to_s.length > 0 then
+                  from = y.genomic.to + d_from
+                  i2 = SegmentPair.new_intron(from, nil, strand, a[2])
+                  itr << i2
+                  segpairs << i2
+                end
+              else
+                y = SegmentPair.parse(x, strand, cflag, [])
+                ex << y
+                segpairs << y
+              end
+            end
+          end
+          @exons = ex
+          @introns = itr
+          @segmentpairs = segpairs
+        end
+        private :parse_segmentpairs
+
+        # Returns exons of the hit.
+        # Returns an array of Bio::Spidey::Report::SegmentPair object.
+        def exons
+          unless defined?(@exons); parse_segmentpairs; end
+          @exons
+        end
+
+        # Returns introns of the hit.
+        # Some of them would contain untranscribed regions.
+        # Returns an array of Bio::Spidey::Report::SegmentPair objects.
+        # (Note that intron data is not always available
+        # according to run-time options of the program.)
+        def introns
+          unless defined?(@introns); parse_segmentpairs; end
+          @introns
+        end
+
+        # Returns segment pairs (exons and introns) of the hit.
+        # Each segment pair is a Bio::Spidey::Report::SegmentPair object.
+        # Returns an array of Bio::Spidey::Report::SegmentPair objects.
+        # (Note that intron data is not always available
+        # according to run-time options of the program.)
+        def segmentpairs
+          unless defined?(@segmentparis); parse_segmentpairs; end
+          @segmentpairs
+        end
+
+        # Returns alignments.
+        # Returns an Array of arrays.
+        # This would be a Bio::Spidey specific method.
+        def align
+          unless defined?(@align); parse_align; end
+          @align
+        end
+
+        # Parses alignment lines.
+        def parse_align_lines(data)
+          misc = [ [], [], [], [] ]
+          data.each do |x|
+            a = x.split(/\r?\n/)
+            if g = a.shift then
+              misc[0] << g
+              (1..3).each do |i|
+                if y = a.shift then
+                  if y.length < g.length
+                    y << ' ' * (g.length - y.length)
+                  end
+                  misc[i] << y
+                else
+                  misc[i] << ' ' * g.length
+                end
+              end
+            end
+          end
+          misc.collect! { |x| x.join('') }
+          left = []
+          if /\A +/ =~ misc[2] then
+            len = $&.size
+            left = misc.collect { |x| x[0, len] }
+            misc.each { |x| x[0, len] = '' }
+          end
+          right = []
+          if / +\z/ =~ misc[2] then
+            len = $&.size
+            right = misc.collect { |x| x[(-len)..-1] }
+            misc.each { |x| x[(-len)..-1] = '' }
+          end
+          body = misc
+          [ left, body, right ]
+        end
+        private :parse_align_lines
+
+        # Parses alignments.
+        def parse_align
+          r = []
+          data = @data
+          while !data.empty?
+            a = []
+            while x = data.shift and !(x =~ /^(Genomic|Exon\s*\d+)\:/)
+              a.push x
+            end
+            r.push parse_align_lines(a) unless a.empty?
+          end
+          @align = r
+        end
+        private :parse_align
+
+        #--
+        # Bio::BLAST::*::Report::Hit compatible methods
+        #++
+
+        # Length of the mRNA (query) sequence.
+        # Same as Bio::Spidey::Report#query_len.
+        def query_len;  mrna.len;        end
+
+        # Identifier of the mRNA (query).
+        # Same as Bio::Spidey::Report#query_id.
+        def query_id;   mrna.entry_id;   end
+
+        # Definition of the mRNA (query).
+        # Same as Bio::Spidey::Report#query_def.
+        def query_def;  mrna.definition; end
+
+        # The genomic (target) sequence length.
+        def target_len; genomic.len;        end
+
+        # Identifier of the genomic (target) sequence.
+        def target_id;  genomic.entry_id;   end
+
+        # Definition of the genomic (target) sequence.
+        def target_def; genomic.definition; end
+
+        alias hit_id     target_id
+        alias len        target_len
+        alias definition target_def
+
+        alias hsps exons
+
+        # Iterates over each exon of the hit.
+        # Yields Bio::Spidey::Report::SegmentPair object.
+        def each(&x) #:yields: segmentpair
+          exons.each(&x)
+        end
+      end #class Hit
+
+      # Returns sequence informationsof the mRNA.
+      # Returns a Bio::Spidey::Report::SeqDesc object.
+      # This would be a Bio::Spidey specific method.
+      def mrna; @hit.mrna; end
+
+      #--
+      #Bio::BLAST::*::Report compatible methods
+      #++
+
+      # Returns number of hits.
+      # Same as hits.size.
+      def num_hits;     @hits.size;     end
+
+      # Iterates over each hits.
+      # Same as hits.each.
+      # Yields a Bio::Spidey::Report::Hit object.
+      def each_hit(&x) #:yields: hit
+        @hits.each(&x)
+      end
+      alias each each_hit
+
+      # Returns definition of the mRNA (query) sequence.
+      def query_def; @hit.mrna.definition; end
+
+      # Returns identifier of the mRNA (query) sequence.
+      def query_id;  @hit.mrna.entry_id;   end
+
+      # Returns the length of the mRNA (query) sequence.
+      def query_len; @hit.mrna.len;        end
+    end #class Report
+
+  end #class Spidey
+end #module Bio
+