bio-polymarker 1.3.2
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- checksums.yaml +7 -0
- data/.travis.yml +24 -0
- data/Gemfile +23 -0
- data/README.md +205 -0
- data/Rakefile +61 -0
- data/SECURITY.md +16 -0
- data/VERSION +1 -0
- data/bin/bfr.rb +128 -0
- data/bin/blast_triads.rb +166 -0
- data/bin/blast_triads_promoters.rb +192 -0
- data/bin/count_variations.rb +36 -0
- data/bin/filter_blat_by_target_coverage.rb +69 -0
- data/bin/filter_exonerate_by_identity.rb +38 -0
- data/bin/find_best_blat_hit.rb +33 -0
- data/bin/find_best_exonerate.rb +17 -0
- data/bin/get_longest_hsp_blastx_triads.rb +66 -0
- data/bin/hexaploid_primers.rb +168 -0
- data/bin/homokaryot_primers.rb +183 -0
- data/bin/mafft_triads.rb +120 -0
- data/bin/mafft_triads_promoters.rb +403 -0
- data/bin/map_markers_to_contigs.rb +66 -0
- data/bin/marker_to_vcf.rb +241 -0
- data/bin/markers_in_region.rb +42 -0
- data/bin/mask_triads.rb +169 -0
- data/bin/polymarker.rb +410 -0
- data/bin/polymarker_capillary.rb +443 -0
- data/bin/polymarker_deletions.rb +350 -0
- data/bin/snp_position_to_polymarker.rb +101 -0
- data/bin/snps_between_bams.rb +107 -0
- data/bin/tag_stats.rb +75 -0
- data/bin/vcfLineToTable.rb +56 -0
- data/bin/vcfToPolyMarker.rb +82 -0
- data/bio-polymarker.gemspec +227 -0
- data/conf/defaults.rb +1 -0
- data/conf/primer3_config/dangle.dh +128 -0
- data/conf/primer3_config/dangle.ds +128 -0
- data/conf/primer3_config/interpretations/dangle_i.dh +131 -0
- data/conf/primer3_config/interpretations/dangle_i.ds +131 -0
- data/conf/primer3_config/interpretations/loops_i.dh +34 -0
- data/conf/primer3_config/interpretations/loops_i.ds +31 -0
- data/conf/primer3_config/interpretations/stack_i.dh +257 -0
- data/conf/primer3_config/interpretations/stack_i.ds +256 -0
- data/conf/primer3_config/interpretations/stackmm_i_mm.dh +257 -0
- data/conf/primer3_config/interpretations/stackmm_i_mm.ds +256 -0
- data/conf/primer3_config/interpretations/tetraloop_i.dh +79 -0
- data/conf/primer3_config/interpretations/tetraloop_i.ds +81 -0
- data/conf/primer3_config/interpretations/triloop_i.dh +21 -0
- data/conf/primer3_config/interpretations/triloop_i.ds +18 -0
- data/conf/primer3_config/interpretations/tstack2_i.dh +256 -0
- data/conf/primer3_config/interpretations/tstack2_i.ds +256 -0
- data/conf/primer3_config/interpretations/tstack_i.dh +256 -0
- data/conf/primer3_config/interpretations/tstack_i.ds +256 -0
- data/conf/primer3_config/interpretations/tstack_tm_inf_i.dh +256 -0
- data/conf/primer3_config/interpretations/tstack_tm_inf_i.ds +256 -0
- data/conf/primer3_config/loops.dh +30 -0
- data/conf/primer3_config/loops.ds +30 -0
- data/conf/primer3_config/stack.dh +256 -0
- data/conf/primer3_config/stack.ds +256 -0
- data/conf/primer3_config/stackmm.dh +256 -0
- data/conf/primer3_config/stackmm.ds +256 -0
- data/conf/primer3_config/tetraloop.dh +77 -0
- data/conf/primer3_config/tetraloop.ds +77 -0
- data/conf/primer3_config/triloop.dh +16 -0
- data/conf/primer3_config/triloop.ds +16 -0
- data/conf/primer3_config/tstack.dh +256 -0
- data/conf/primer3_config/tstack2.dh +256 -0
- data/conf/primer3_config/tstack2.ds +256 -0
- data/conf/primer3_config/tstack_tm_inf.ds +256 -0
- data/lib/bio/BFRTools.rb +465 -0
- data/lib/bio/BIOExtensions.rb +153 -0
- data/lib/bio/PolyploidTools/ChromosomeArm.rb +63 -0
- data/lib/bio/PolyploidTools/ExonContainer.rb +245 -0
- data/lib/bio/PolyploidTools/Marker.rb +175 -0
- data/lib/bio/PolyploidTools/Mask.rb +116 -0
- data/lib/bio/PolyploidTools/NoSNPSequence.rb +292 -0
- data/lib/bio/PolyploidTools/PrimerRegion.rb +30 -0
- data/lib/bio/PolyploidTools/SNP.rb +804 -0
- data/lib/bio/PolyploidTools/SNPMutant.rb +86 -0
- data/lib/bio/PolyploidTools/SNPSequence.rb +55 -0
- data/lib/bio/db/blast.rb +114 -0
- data/lib/bio/db/exonerate.rb +333 -0
- data/lib/bio/db/primer3.rb +820 -0
- data/lib/bio-polymarker.rb +28 -0
- data/test/data/7B_amplicon_test.fa +12 -0
- data/test/data/7B_amplicon_test.fa.fai +1 -0
- data/test/data/7B_amplicon_test_reference.fa +110 -0
- data/test/data/7B_amplicon_test_reference.fa.fai +3 -0
- data/test/data/7B_marker_test.txt +1 -0
- data/test/data/BS00068396_51.fa +2 -0
- data/test/data/BS00068396_51_blast.tab +4 -0
- data/test/data/BS00068396_51_contigs.aln +1412 -0
- data/test/data/BS00068396_51_contigs.dnd +7 -0
- data/test/data/BS00068396_51_contigs.fa +8 -0
- data/test/data/BS00068396_51_contigs.fa.fai +4 -0
- data/test/data/BS00068396_51_contigs.fa.nhr +0 -0
- data/test/data/BS00068396_51_contigs.fa.nin +0 -0
- data/test/data/BS00068396_51_contigs.fa.nsq +0 -0
- data/test/data/BS00068396_51_contigs.nhr +0 -0
- data/test/data/BS00068396_51_contigs.nin +0 -0
- data/test/data/BS00068396_51_contigs.nsq +0 -0
- data/test/data/BS00068396_51_exonerate.tab +6 -0
- data/test/data/BS00068396_51_for_polymarker.txt +1 -0
- data/test/data/BS00068396_51_genes.txt +14 -0
- data/test/data/IWGSC_CSS_1AL_scaff_1455974.fa +112 -0
- data/test/data/IWGSC_CSS_1AL_scaff_1455974_aln_contigs.fa +2304 -0
- data/test/data/IWGSC_CSS_1AL_scaff_1455974_aln_contigs.fa.fai +11 -0
- data/test/data/LIB1716.bam +0 -0
- data/test/data/LIB1716.bam.bai +0 -0
- data/test/data/LIB1719.bam +0 -0
- data/test/data/LIB1719.bam.bai +0 -0
- data/test/data/LIB1721.bam +0 -0
- data/test/data/LIB1721.bam.bai +0 -0
- data/test/data/LIB1722.bam +0 -0
- data/test/data/LIB1722.bam.bai +0 -0
- data/test/data/PST130_7067.csv +1 -0
- data/test/data/PST130_7067.fa +2 -0
- data/test/data/PST130_7067.fa.fai +1 -0
- data/test/data/PST130_7067.fa.ndb +0 -0
- data/test/data/PST130_7067.fa.nhr +0 -0
- data/test/data/PST130_7067.fa.nin +0 -0
- data/test/data/PST130_7067.fa.not +0 -0
- data/test/data/PST130_7067.fa.nsq +0 -0
- data/test/data/PST130_7067.fa.ntf +0 -0
- data/test/data/PST130_7067.fa.nto +0 -0
- data/test/data/PST130_reverse_primer.csv +1 -0
- data/test/data/S22380157.fa +16 -0
- data/test/data/S22380157.fa.fai +1 -0
- data/test/data/S22380157.vcf +67 -0
- data/test/data/S58861868/LIB1716.bam +0 -0
- data/test/data/S58861868/LIB1716.sam +651 -0
- data/test/data/S58861868/LIB1719.bam +0 -0
- data/test/data/S58861868/LIB1719.sam +805 -0
- data/test/data/S58861868/LIB1721.bam +0 -0
- data/test/data/S58861868/LIB1721.sam +1790 -0
- data/test/data/S58861868/LIB1722.bam +0 -0
- data/test/data/S58861868/LIB1722.sam +1271 -0
- data/test/data/S58861868/S58861868.fa +16 -0
- data/test/data/S58861868/S58861868.fa.fai +1 -0
- data/test/data/S58861868/S58861868.vcf +76 -0
- data/test/data/S58861868/header.txt +9 -0
- data/test/data/S58861868/merged.bam +0 -0
- data/test/data/S58861868/merged_reheader.bam +0 -0
- data/test/data/S58861868/merged_reheader.bam.bai +0 -0
- data/test/data/Test3Aspecific.csv +2 -0
- data/test/data/Test3Aspecific_contigs.fa +6 -0
- data/test/data/bfr_out_test.csv +5 -0
- data/test/data/chr1A_C1145499T/chr1A_C1145499T.csv +1 -0
- data/test/data/chr1A_G540414846C/chr1A_G540414846C.csv +1 -0
- data/test/data/chr1A_G540414846C/chr1A_G540414846C.fa +2 -0
- data/test/data/chr1A_T517634750C/chr1A_T517634750C.csv +1 -0
- data/test/data/chr2D_C112180134A/chr2D_C112180134A.csv +1 -0
- data/test/data/chr4D_C14473543T/chr4D_C14473543T.csv +1 -0
- data/test/data/chr4D_C14473543T/chr4D_C14473543T.fa +2 -0
- data/test/data/headerMergeed.txt +9 -0
- data/test/data/headerS2238015 +1 -0
- data/test/data/mergedLibs.bam +0 -0
- data/test/data/mergedLibsReheader.bam +0 -0
- data/test/data/mergedLibsSorted.bam +0 -0
- data/test/data/mergedLibsSorted.bam.bai +0 -0
- data/test/data/patological_cases5D.csv +1 -0
- data/test/data/primer_3_input_header_test +5 -0
- data/test/data/short_primer_design_test.csv +10 -0
- data/test/data/some_tests/some_tests.csv +201 -0
- data/test/data/test_from_mutant.csv +3 -0
- data/test/data/test_iselect.csv +196 -0
- data/test/data/test_iselect_reference.fa +1868 -0
- data/test/data/test_iselect_reference.fa.fai +934 -0
- data/test/data/test_primer3_error.csv +4 -0
- data/test/data/test_primer3_error_contigs.fa +10 -0
- data/test/test_bfr.rb +135 -0
- data/test/test_blast.rb +47 -0
- data/test/test_exon_container.rb +17 -0
- data/test/test_exonearate.rb +48 -0
- data/test/test_integration.rb +76 -0
- data/test/test_snp_parsing.rb +121 -0
- data/test/test_wrong_selection.sh +5 -0
- metadata +356 -0
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738
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740
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741
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760
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864
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865
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866
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870
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912
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913
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915
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925
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927
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929
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930
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931
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932
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934
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