bio-polymarker 1.3.2

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Files changed (177) hide show
  1. checksums.yaml +7 -0
  2. data/.travis.yml +24 -0
  3. data/Gemfile +23 -0
  4. data/README.md +205 -0
  5. data/Rakefile +61 -0
  6. data/SECURITY.md +16 -0
  7. data/VERSION +1 -0
  8. data/bin/bfr.rb +128 -0
  9. data/bin/blast_triads.rb +166 -0
  10. data/bin/blast_triads_promoters.rb +192 -0
  11. data/bin/count_variations.rb +36 -0
  12. data/bin/filter_blat_by_target_coverage.rb +69 -0
  13. data/bin/filter_exonerate_by_identity.rb +38 -0
  14. data/bin/find_best_blat_hit.rb +33 -0
  15. data/bin/find_best_exonerate.rb +17 -0
  16. data/bin/get_longest_hsp_blastx_triads.rb +66 -0
  17. data/bin/hexaploid_primers.rb +168 -0
  18. data/bin/homokaryot_primers.rb +183 -0
  19. data/bin/mafft_triads.rb +120 -0
  20. data/bin/mafft_triads_promoters.rb +403 -0
  21. data/bin/map_markers_to_contigs.rb +66 -0
  22. data/bin/marker_to_vcf.rb +241 -0
  23. data/bin/markers_in_region.rb +42 -0
  24. data/bin/mask_triads.rb +169 -0
  25. data/bin/polymarker.rb +410 -0
  26. data/bin/polymarker_capillary.rb +443 -0
  27. data/bin/polymarker_deletions.rb +350 -0
  28. data/bin/snp_position_to_polymarker.rb +101 -0
  29. data/bin/snps_between_bams.rb +107 -0
  30. data/bin/tag_stats.rb +75 -0
  31. data/bin/vcfLineToTable.rb +56 -0
  32. data/bin/vcfToPolyMarker.rb +82 -0
  33. data/bio-polymarker.gemspec +227 -0
  34. data/conf/defaults.rb +1 -0
  35. data/conf/primer3_config/dangle.dh +128 -0
  36. data/conf/primer3_config/dangle.ds +128 -0
  37. data/conf/primer3_config/interpretations/dangle_i.dh +131 -0
  38. data/conf/primer3_config/interpretations/dangle_i.ds +131 -0
  39. data/conf/primer3_config/interpretations/loops_i.dh +34 -0
  40. data/conf/primer3_config/interpretations/loops_i.ds +31 -0
  41. data/conf/primer3_config/interpretations/stack_i.dh +257 -0
  42. data/conf/primer3_config/interpretations/stack_i.ds +256 -0
  43. data/conf/primer3_config/interpretations/stackmm_i_mm.dh +257 -0
  44. data/conf/primer3_config/interpretations/stackmm_i_mm.ds +256 -0
  45. data/conf/primer3_config/interpretations/tetraloop_i.dh +79 -0
  46. data/conf/primer3_config/interpretations/tetraloop_i.ds +81 -0
  47. data/conf/primer3_config/interpretations/triloop_i.dh +21 -0
  48. data/conf/primer3_config/interpretations/triloop_i.ds +18 -0
  49. data/conf/primer3_config/interpretations/tstack2_i.dh +256 -0
  50. data/conf/primer3_config/interpretations/tstack2_i.ds +256 -0
  51. data/conf/primer3_config/interpretations/tstack_i.dh +256 -0
  52. data/conf/primer3_config/interpretations/tstack_i.ds +256 -0
  53. data/conf/primer3_config/interpretations/tstack_tm_inf_i.dh +256 -0
  54. data/conf/primer3_config/interpretations/tstack_tm_inf_i.ds +256 -0
  55. data/conf/primer3_config/loops.dh +30 -0
  56. data/conf/primer3_config/loops.ds +30 -0
  57. data/conf/primer3_config/stack.dh +256 -0
  58. data/conf/primer3_config/stack.ds +256 -0
  59. data/conf/primer3_config/stackmm.dh +256 -0
  60. data/conf/primer3_config/stackmm.ds +256 -0
  61. data/conf/primer3_config/tetraloop.dh +77 -0
  62. data/conf/primer3_config/tetraloop.ds +77 -0
  63. data/conf/primer3_config/triloop.dh +16 -0
  64. data/conf/primer3_config/triloop.ds +16 -0
  65. data/conf/primer3_config/tstack.dh +256 -0
  66. data/conf/primer3_config/tstack2.dh +256 -0
  67. data/conf/primer3_config/tstack2.ds +256 -0
  68. data/conf/primer3_config/tstack_tm_inf.ds +256 -0
  69. data/lib/bio/BFRTools.rb +465 -0
  70. data/lib/bio/BIOExtensions.rb +153 -0
  71. data/lib/bio/PolyploidTools/ChromosomeArm.rb +63 -0
  72. data/lib/bio/PolyploidTools/ExonContainer.rb +245 -0
  73. data/lib/bio/PolyploidTools/Marker.rb +175 -0
  74. data/lib/bio/PolyploidTools/Mask.rb +116 -0
  75. data/lib/bio/PolyploidTools/NoSNPSequence.rb +292 -0
  76. data/lib/bio/PolyploidTools/PrimerRegion.rb +30 -0
  77. data/lib/bio/PolyploidTools/SNP.rb +804 -0
  78. data/lib/bio/PolyploidTools/SNPMutant.rb +86 -0
  79. data/lib/bio/PolyploidTools/SNPSequence.rb +55 -0
  80. data/lib/bio/db/blast.rb +114 -0
  81. data/lib/bio/db/exonerate.rb +333 -0
  82. data/lib/bio/db/primer3.rb +820 -0
  83. data/lib/bio-polymarker.rb +28 -0
  84. data/test/data/7B_amplicon_test.fa +12 -0
  85. data/test/data/7B_amplicon_test.fa.fai +1 -0
  86. data/test/data/7B_amplicon_test_reference.fa +110 -0
  87. data/test/data/7B_amplicon_test_reference.fa.fai +3 -0
  88. data/test/data/7B_marker_test.txt +1 -0
  89. data/test/data/BS00068396_51.fa +2 -0
  90. data/test/data/BS00068396_51_blast.tab +4 -0
  91. data/test/data/BS00068396_51_contigs.aln +1412 -0
  92. data/test/data/BS00068396_51_contigs.dnd +7 -0
  93. data/test/data/BS00068396_51_contigs.fa +8 -0
  94. data/test/data/BS00068396_51_contigs.fa.fai +4 -0
  95. data/test/data/BS00068396_51_contigs.fa.nhr +0 -0
  96. data/test/data/BS00068396_51_contigs.fa.nin +0 -0
  97. data/test/data/BS00068396_51_contigs.fa.nsq +0 -0
  98. data/test/data/BS00068396_51_contigs.nhr +0 -0
  99. data/test/data/BS00068396_51_contigs.nin +0 -0
  100. data/test/data/BS00068396_51_contigs.nsq +0 -0
  101. data/test/data/BS00068396_51_exonerate.tab +6 -0
  102. data/test/data/BS00068396_51_for_polymarker.txt +1 -0
  103. data/test/data/BS00068396_51_genes.txt +14 -0
  104. data/test/data/IWGSC_CSS_1AL_scaff_1455974.fa +112 -0
  105. data/test/data/IWGSC_CSS_1AL_scaff_1455974_aln_contigs.fa +2304 -0
  106. data/test/data/IWGSC_CSS_1AL_scaff_1455974_aln_contigs.fa.fai +11 -0
  107. data/test/data/LIB1716.bam +0 -0
  108. data/test/data/LIB1716.bam.bai +0 -0
  109. data/test/data/LIB1719.bam +0 -0
  110. data/test/data/LIB1719.bam.bai +0 -0
  111. data/test/data/LIB1721.bam +0 -0
  112. data/test/data/LIB1721.bam.bai +0 -0
  113. data/test/data/LIB1722.bam +0 -0
  114. data/test/data/LIB1722.bam.bai +0 -0
  115. data/test/data/PST130_7067.csv +1 -0
  116. data/test/data/PST130_7067.fa +2 -0
  117. data/test/data/PST130_7067.fa.fai +1 -0
  118. data/test/data/PST130_7067.fa.ndb +0 -0
  119. data/test/data/PST130_7067.fa.nhr +0 -0
  120. data/test/data/PST130_7067.fa.nin +0 -0
  121. data/test/data/PST130_7067.fa.not +0 -0
  122. data/test/data/PST130_7067.fa.nsq +0 -0
  123. data/test/data/PST130_7067.fa.ntf +0 -0
  124. data/test/data/PST130_7067.fa.nto +0 -0
  125. data/test/data/PST130_reverse_primer.csv +1 -0
  126. data/test/data/S22380157.fa +16 -0
  127. data/test/data/S22380157.fa.fai +1 -0
  128. data/test/data/S22380157.vcf +67 -0
  129. data/test/data/S58861868/LIB1716.bam +0 -0
  130. data/test/data/S58861868/LIB1716.sam +651 -0
  131. data/test/data/S58861868/LIB1719.bam +0 -0
  132. data/test/data/S58861868/LIB1719.sam +805 -0
  133. data/test/data/S58861868/LIB1721.bam +0 -0
  134. data/test/data/S58861868/LIB1721.sam +1790 -0
  135. data/test/data/S58861868/LIB1722.bam +0 -0
  136. data/test/data/S58861868/LIB1722.sam +1271 -0
  137. data/test/data/S58861868/S58861868.fa +16 -0
  138. data/test/data/S58861868/S58861868.fa.fai +1 -0
  139. data/test/data/S58861868/S58861868.vcf +76 -0
  140. data/test/data/S58861868/header.txt +9 -0
  141. data/test/data/S58861868/merged.bam +0 -0
  142. data/test/data/S58861868/merged_reheader.bam +0 -0
  143. data/test/data/S58861868/merged_reheader.bam.bai +0 -0
  144. data/test/data/Test3Aspecific.csv +2 -0
  145. data/test/data/Test3Aspecific_contigs.fa +6 -0
  146. data/test/data/bfr_out_test.csv +5 -0
  147. data/test/data/chr1A_C1145499T/chr1A_C1145499T.csv +1 -0
  148. data/test/data/chr1A_G540414846C/chr1A_G540414846C.csv +1 -0
  149. data/test/data/chr1A_G540414846C/chr1A_G540414846C.fa +2 -0
  150. data/test/data/chr1A_T517634750C/chr1A_T517634750C.csv +1 -0
  151. data/test/data/chr2D_C112180134A/chr2D_C112180134A.csv +1 -0
  152. data/test/data/chr4D_C14473543T/chr4D_C14473543T.csv +1 -0
  153. data/test/data/chr4D_C14473543T/chr4D_C14473543T.fa +2 -0
  154. data/test/data/headerMergeed.txt +9 -0
  155. data/test/data/headerS2238015 +1 -0
  156. data/test/data/mergedLibs.bam +0 -0
  157. data/test/data/mergedLibsReheader.bam +0 -0
  158. data/test/data/mergedLibsSorted.bam +0 -0
  159. data/test/data/mergedLibsSorted.bam.bai +0 -0
  160. data/test/data/patological_cases5D.csv +1 -0
  161. data/test/data/primer_3_input_header_test +5 -0
  162. data/test/data/short_primer_design_test.csv +10 -0
  163. data/test/data/some_tests/some_tests.csv +201 -0
  164. data/test/data/test_from_mutant.csv +3 -0
  165. data/test/data/test_iselect.csv +196 -0
  166. data/test/data/test_iselect_reference.fa +1868 -0
  167. data/test/data/test_iselect_reference.fa.fai +934 -0
  168. data/test/data/test_primer3_error.csv +4 -0
  169. data/test/data/test_primer3_error_contigs.fa +10 -0
  170. data/test/test_bfr.rb +135 -0
  171. data/test/test_blast.rb +47 -0
  172. data/test/test_exon_container.rb +17 -0
  173. data/test/test_exonearate.rb +48 -0
  174. data/test/test_integration.rb +76 -0
  175. data/test/test_snp_parsing.rb +121 -0
  176. data/test/test_wrong_selection.sh +5 -0
  177. metadata +356 -0
data/test/data/BS00068396_51_contigs.dnd ADDED
@@ -0,0 +1,7 @@
1
+ (
2
+ (
3
+ 2AS_5222932:0.20551,
4
+ 2BS_5163353:0.19641)
5
+ :0.57378,
6
+ 2BS_5245544:0.21414,
7
+ 2DS_5334799:0.22137);
data/test/data/BS00068396_51_contigs.fa ADDED
@@ -0,0 +1,8 @@
1
+ >2AS_5222932 6364 267569 1716390+,...,724070+
2
+ CAACAACAACAACAACAACAACAACAACAACAACAACAACAACAACAACAACAACAACAACAACAACAACTAAAAGATATCGGGGAGGCGGATACACAGCTTACATCACACCCCGTGCTACAAGCAACTAAAGATTGGAAAAAGAGAAGCCCGGTCGCATAGTGAGAAGTCTTCCATCTCGAAACATGTGCGAAGAAGAAGATCGCCAAGGGAAATGCCAACTGACTTGGCAACAAACTCCCCGTACGCCGACCCTGAGACACAAGAAGACGCCACACCCCCTACACCCTCACTGAAGGAGGACTTTACCCCTCGTCCTAGCTCAAAGGACGCCGCACCATTGAGCGAGGGATGTGTTCAACCTAGAACAGGAATGGTGTGGTCTTGAGGTCACAGGAGCCGAGCACATACACGCGACCCGCCACACCGCAGCGGGCAAACCATGGTGGCCATGAAGAAACGTCCGCACAATGCCTGGAACAAGAAGGGGGAACCTGTAGGAGAGGTTCACGGAAATCGAAGAAGCGGCAAGCTCAAGGCGGACAACAAGCACACAATATAAGCACAAGGAAGAAGGACCGGGTAACTACATGCCGCCACGAACAAACGTCGGGAAGGGGAACCCTTTCCCCTCCCACCCAGGTTCAAGGGTGATGAACCACCGACGAATAGGGCAGCGAATACCTCACTGAAGATAGTATACTCAACGCTTCACACAAACTGTCGCTCGAAACCCACAACAACCACAAAATTAACCACATGGCACCACACACCAGCCTCTCCTTGCCAAACGACTCCCCCAGGGAGGTAACAACACCGAGCGTCGCCGCCGCCGCATCCACAGGGATAACAGGAATTAACCTTGGGTCAAAGAAGAGGGGTAAGGAGAAGGGATCACGTCGGTGCCTCCAAGGAGGGGAGCAGCGCCAGCAGGCGGTGTCACCGACGTGGTCGGCCAAGCCACCAAAGGATTTCTCTCGAACCCAACTCACACCACCATGCCCGGCGATACAAGATCACCACCACGAGATCCGGCCGGAAGTGGACCAAATGGGAAAAAGGAGTGACCTTCAGATATGACGCCAGAAGAACCGCCGGAGCAGACCGCCGACCATGAACCGCACCTGTCGTCGCCGCACTGCCCACACGGCTGAGGACGCCGACCCTCTCGTGTGAGCATCGCCAGCCGCGAAGACGGCGTCGCCTCTCCAAGAAGGTTCGCCGCCTGGAGCCCAAAGCCCTCCGCCGAGACCGGAGCGACGAAATCCCCGTTGCCACCTTCACCGTAGGCTGCGCGGGGTTGCCCGGCAACCAGTGGCGGACCTAGAATCATAGGCTTGGGGGTGCCATAGTTCAATGTTCACATTAAACGTCATCATAATCTCAATAAATAGTCTTAAAATAGCAACAACTTGATGAATATGTAGCGTGAGGTATAGAGTAAAATTTATTTTACAACTTTGAAGGGGTGCCTGGCACCCACGGCCTCAAATTAGTTATGGCGGTGGCCAAGGTTTGATATCATCTTGGAATCGTCCACGGGAGCGATTCGGTGACCAAGGTCTTTTCTGTAAACTAAACTTATTCCCTATGCTGGCCTTATCAATTCCGAGTTAATCAAACCCATCAAAATCGAATTGAAGGAGCAAATTGATAAAATACGATTTTCTGCTTTGACACATCATCATTGTTCTCCTTTGATGGAACATCTTGACCATCAAACATCCACATTTAACCCCTTAAAAGAACACATTCTACATTTAAGCTAGCGCTTTCTTCTGTCTTGCCCCCTTGCCAAAAACACAAGATATTCCACGCTGCCAAACACACCAATCGGAAAAAGGCAATTTTCACTTTTCATCGATTAGAAGCAGCTCCGAAAAGGAAAAGAAGCAACCATCTCTCCCCACCCCACCCCGGCCCTGCTCCCCGCCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTGGGCGGGGGTGAGTGGCGAGCCTCCCTCGCCGCCTTGCGCTGCACAGGAACAAGGCGAGAGCGATCGGCGTCGGGATGCTGAGGCGGCGGCTGAGCTCCCTTCTCCTCCGATCCCCGTGCTCCTCCTCCGCCGTCGCTTCCTCCTACCAGCAGCAGCAGCACCTCCTCCTCCCCACCCACGCCGAGGTAACCCCCACGGTTCCCCTTTCTTTCCCTCCCGCGCATTTCGTCGAACGCCTCGCGCGCGCGCACAACGTGGCCGGACCGGATCTGGCCTGCACTCCTTTCTCGCTCCGTTTGGTTCAATGCGTGCCTGCCGTTCCTAGATGCTCATCCGCCTGACTGTTTTCCTCGCAGAAACCGCCGGCCCTGAATCTGCTCAGGCTCTTCACATCCCTAGCTGTACGTTTGTTCCCAAACCCGCATTCTTATTATTTGTGACAACTATGCAGACTGCATGTGAATAATTGGTGGTGTGTCCTCTGTTCATTCTGTAGGGAACCGATGGGGACAGGCCGTTCATCGCTTTTGTCTTGGGTAGTTTTTCCCTGCTTTTACTCCCAAAATGTAGCAGGATTGTTGTTTTCGTTCTTCGAAACGGAGGGCAAAGCTTTGCAGGGTCGGTTTTACATTTATAAAGTTCCATGCATATCTGTGTAGAGTAAACCGAGGAACCCTTGCCTCAATGATAATCAGTGCTGTTTCACATTTATAATCGCTTTCGTCTTAGGTAAAAAAATAAATCTACTAGTACTCCCCCAATATTGCAGGGTTGGTTTCACATTTACATACTCCATGCATAACCTGTGTAGAGTAAATTGAGGATCACTTCTATCAATGATAACCAGTGCTGTTTCAAGTTGGATTGTGTTGTGGCGCTCAAATTTCAGTTGTTCACACGACTAATCAAGATCCTGCATATAGGGGGACCTGGGAGTGGGAAAGGAACGCAATGTAGTAGGATCGCTTCGGATTTTGGATTTGCTCATGTGAGTGCTGGTGACCTTCTACGGAATGAAATAGCTTCTGGCACTGATCATGGGTAATTCTTCTTTCCGTTTGATCCTCTGACAGCCTTATAATGACCCACACGCACATGCATGGTTCGGTGAATTGATTTTTCAGTTTCATGGCATTTGCCAGGGAGTGGATCCTAGAGATAATAAGGGAAGGGAGGATTGTTCCATCAGAGATAACTGTTGAACTCGTCAGAAAAGCAATTGAATCAAGCTCTGCTAAAAGGGTTCTTATTGATGGTTTCCCAAGGTGTGAGGAAAACAGAATTGCCTTTGAAAAAATAGTAAGTTTTCTGTTTGGCTTGCATTACTGGTCCAGAACTTTCTATTGATTGAAATAGTATGTGATGAGATGGATAAGTCCATGCTGTTCATCAGTTTCTGTTCAATTTTTGGGAAGGCTAATAACAGGTGAATCTTCACGTTTTCTAGCGCTCTTGGCTGTAACCATTTGTGTATACACACTTGTCTTTACATTTTTACGCCAATATGCTGGCCCAAAACTGCCAGTGTACAGTATGCAATCAGCAGTTTCTTATGCATGTATTTTCTTTTAGTAGCAAATCAGTAACTATGTCCTGCTCTAGAGTGATGCACTTTTAGGATTTATGCAGTAGTCCACAGTTATGTCAGCCCCACTATGGTGGATGTATAGTTGCATCTCAACAACTACTACAAGCATTTATATCAAAGAATGTGTGTGCTGTGGTCGGGCAATTAGAAGACACTGGCATACCAACTAAGATATGTTTCATTGTTGTCCACTCAATAATTGCTAGTCAGATAGATGTAGATTGCATGATCCTGTTCTGATATGTTGACTCATAAATTTCAGACTGGAACTGAACCAGACCTTGTGCTTTTCTTTGACTGTCCTGAGGATGAGATGGTTAAACGTCTCCTAAGCCGTAACCAGGTTGGTCTTCGTATTATCTCTGGAGTAAATTGTGCTAGATATATATACACTATATGAGTACTGTACATTTCCCAATTCTTATTCGGAACTTACTCCATATGTGATTTTCTGAGATTTTATTTCCCAGCCGTTACTTTCGGCTTTGACAATTATCTTCTTATTACTCCCTCTCTTAACTAACATGAAATATAGCATTCCTAAAGGATAGTCCTTGTGGATTGCCACAAATGATTTTCTTCTTCATATGTCATTTAAATTGAAGAGAAGAAACACTAATCTAATTAGTGTAGTTCGTGCATATCAAAATGGGAGGTTGATTAATGCTCTAGCGGAGTTTCCTTTCAAAAATATCACATTAGATTGATCTCTTTATTGTTTTTGTTTTTTCGATTATCTCATGTATGCGTCTTAGGGACGAGTTGATGATAACATTGAGACAATCAAGAAGCGTCTAAAAGTATTCGAGAGTTTGAATCTTCCTGTTGTTGAGTACTACTCTTCAAGGGGAAAGGCACACAAGGTAATTAATCTATTTATTTTATGCCGAGTGTCCTGGACAGGAGCACTTCTTGTACCTTTCTTTTATGAACCGTACCTTTACCTTTTGACATGCATTTTCCTATTTCCTTCACACGGAAACAGAGCTCTACCTTCTGTTGTAGAAGTTGATCCAAAAGGCTTCTTAATGTGCAACATTAAACTATACCTGGCTTATTTTCTTGAACTTTTACAAATGCCAATATATATTTGAGAAACATCAAACATATGGGTTACCAATGTATAAATAGCAGGATAGTGTTTCCAGGGAATCATAGGACAGAGAGGCAGAGCCAGGATGCGATTCCCTCGTGTTCTGAATTCATATAACTGCAAGACCATTTTTCTATGGTGTGGGGAGGGGAGTGAGATCAGAGATCTGCGTATTTATTTGATATTAGATGAGTGTCTGCAATTCACTCATTTTGGAAGAAATTGCTAATCTCTTCAGAAGGGGTACATATCAGGGATATAACACAGTAACATATTCTGTATACCACTCATATTCAGGATCTGTAGGATGTCTTATTTATTTTTGAGACTTGACCTTTAGGGTGTCTAAGAGTACTTGTTGAACTCCATGCAGATAAATGCAACGGGAACTGAAGATGAAATCTTTGAAGCAGTTCGCAAGTTGTTTTCCTCCTTAAGGTAAATCCTGATGTTTAAGACACGAAATTCCTCTCATTTACTGTGACTATGTCCGCTCTCCTGAAGGAGATGCACATTCTTGCATCATCTGATTTCTGATTGCTTGCATATTATTTTCTAGTCCCAATTACTGTAGTTCTGAAAGCTTTTCAGTCCTCACATATTTAGCATATCCCAACCCTCAGTCCTCACATATATTGCAGCACCCACACTTTTTCAATACATTAACAAGTGATGGCATGTTCTTTATACTAAGCCTGCCATTGCAGCTAGGGCAGGCCGTCGCTATAAGCAGAAGGGTATTGCTTGTAGGTACAAAGAGCTTGCGTTCCTGAATCCTTGCCATTTGTGTTCACTGTGCACAATAAAGAAAGACAAACTGACAAGCAAAATGAACATGGTAGCATTTATCTTACTGATTGATGGATAAGGAAGAAGAGGTCCAGGATGTTGCTACGTCGCAAACTTTGTCATCTAGCTGACTCGCCGAACCCTCGCCTGTAGCTAGTTTCTCGACACGTTTGACTAAGCAACGCTAGCAACTTGCGGTTGTCCCAGCTTATGATAAAGATGCACTCCTACTTTGTTGCTACATATACACAAGGTAGTTGCAATCATTTGACCATTTCACCCTGTTTGCAGGCTATGAGGAGGCTGTCGTCTTCCGCGCGAGCGGGGGGAATTGGAATTACGCCTGGCGGAAATTCTCTCTCCCTCTTTTCTTTTACCTTTACATCTCTTAGCATAATAATTGTACATATTCTGATACACGGACCATTCCTGTGAAAACAGAGGGCCCCTGGTGAAACGCCCAGCCTGCCTGCTATGATAAACCCTGACCCTTGCGCTCTCGTCACGCGCCACGGTATACGATACTTCAGACTGCAGCAAAACTGCTACAACGATATTTTTTTTCTGCCAACACCCTACAAATATATGTCTGCCGTGTTTTTTTTACCAGGATAATATAGTTGTATGTATGTGCGCCGTGCTTGTGTTGTGACGATTTGCTGGGTATATATCTATGGAGAGATGAGGTGAGAGCTGCAGGCGTCCGATAAACTAGTGATTTGACTGGAGGAAAAAGGCCGTGTACGTTTGGATTTAGTTAATGTGAACTGCCGGCGTGTTATTGTCAGTTCTTGACCGGCCGTTTAGCAGTAGCTGATGCCATCTTCAAACGCTCGCCAAAAAAAAAAA
3
+ >2BS_5245544 8836 99090 5145698+,...,1324530+
4
+ CGCCACTGCCTCCAATTCACACATTCATGAAGTTCCGGATGTAGTTTTGGTCAAGTTTGAACCCATTTTTCCCTTTCAAAAGTCTTCATTACAAGTGTGAAGATCTCTGTCTATAGATTTGCCTAAAATGTACATTCATACATTCTGCTAGGTTCTACTCCCTCCGTCTCAAAATAAGTGACTCAACTTTGTACTAGCTCTAGTATAAATTTGTACTAAGCTCAAGACACTTATTCTGGGACGAAGGGACTATAATGTAATTATATATTGCGGGAGAAAATAATAGTCACATTTATGTGTGTCTTTTTTCCGCGGGGGCACATTTTATGTTTTCTTGATTGTGTGGATGTGCAAAACGCCATCTTTTTTCGGAGACAAGCACTTCAACTCCCCCTCTTTTGGGGGGATGGAAAGATATAGATGAGAAATTTTTGGCTGGCTATGACATGCCCGGTTGGAGTTTTGCTCCCCAAAAGTCTTCACATGACCTCACTCCATGGAGTCATGGCAGCTTGGTGCAGTCGATGCCGACGTTGCTCGTGACGAATCATCTTTCTCTCCATGCCTAGATTCCAACACAAATTTCAGGTTCACCTCAACCTCTTCATTCCCCGGATTATGTGCGTGCGATCGAGACAGTTTTCACATGCCCGGATTATGTACCTAGCCAAAAAAATAATAATAACGCTGCGATTTTTCCGCGTTTCGCGATCATCTATGTTGCCTTCGCGTGTGTGTTCATATCAATTTCGCGTGTTCCACTCGCAATCATGCGTGCTGCCTTTGCATATATTCCAATCGAATTCGTGTGTTCCATTCCTAGGATCCAAAGAGGCCCTCCGAGAAGTCCACCTGTCAGCGCGACGATGACGATGACGACACGCCGGCTCTGTAGCAAGTGCCAGGTGCCCGCGCTCCATGGCTTGCAAGGCGATGATGCAGGCAAGAAAAATGAGTCAATAGTAGCAGAGCTTTCTCCTCCCGTGGAGTGCACAGACAGACAATCTTTTGGAAACGGGGAGTTTTTAGATTAGACTTGGCGTGCAAGCGCCGGCGCTCCACATTTTTTTTTTCTTTCAAGATCGCATCAGCTAAACCTAAACGGCCGGTCAAGAACTGACAAAAACACGCCGGCGGTTCACACTAACTAAATCCAAACGTACACTCTCTTTTCCCTTGAGTCAAATCACTAGTTTATCGAACGCCTGCAGTTCTCACCTCGTATACAAATAGCAGATTGTCACCAGACAAGCACGGCGCACATACAACAATATCATATTCTAGTAAAAAAAACAACAACAGGGCAGGCATATACTCCCTCCGTCCAAAAATACTTGTCATCAAAATAGATAAAAGAGATGTATCTAGAACTAAAATATGTCCCATTTTGATGACAAGTATTTCCAGACGGAGGGAGTATATTGTTAGGGTGTTGGAAGAAAAATATCATTGTAGCAGTATTGCTGCCATACCAGCGTATCATGCGCTATACCGTGGCGCGCGACGGGAGCGTGAGGGTTAGGGTTTATCATAGCGGGCTGAGCGTTTCACCGGGGGCCCTCTGTTTTCACAGGCATGGACCGTGTATCAGAATATGTACAATTATTATGCCAAGACATGTAAAAGTAAATAAGGAAAGGAAGAGAATTTCCGCCAGGCGTAACTCTGATTCCCCCGGCTCCTGCGGAAGACGACGACAGCCTCCTCATAGCCTGCAAACAGGGCGAAACGGTCAAATCATTGCAACTCTCTTTTACTTATTATATTAGATAGAAGAAGATGCCGGTATTGCTTAGTCAAACGTGTCGAGAAACTAGCTACATGGAAAGGGTTCGGCGAGTCAGCTAGATGACAAAGTTAGCTACGTTGCAACCCCCTGGACCTCTTCTTCCTTATCCATCAATCAACAAGGCAGATGCTACTTTGTTCATTCATTTTGCTTGCCAGTTTGTCTTTCTTTATTGTGCACAATGAACACAAATGGCAAGGATCAGAAATTCAGTCTCTTTCTACCTACAAGCAATATCCTTTTGCTTATAGCGACGGCCTGCCCTAGCTGCAATGGCAGGCTTGGTATAAAGAACTCGTCATCACTTGTTAATGTATTGAAAAGTGTGGGTGCTGCAGCTTAGGCATTTAATTGGAAGGGTTAGGATATGCTAAATATGTGAAGACTGAAAACTTTCAGAACTAATTTGGCACTAGAAAATGATATGCAAGCAATCAGAAATCAGATGATGCAAGAATGTGCACCTCCTTCAGGAGAGCGGACATAGTCACAGTATATGAGAGGAATTCCGTGTCTTGAACATCAGGATTTACCTTAAGGTGGAAAACAACTTGCGAACTGCTTCAAAGATTTCATCTTCAGTTCCCGTTGCGTTTATCTGCATGGAGTTCAACAAGTACTCTTAGACACCCTAAAGGTCAAGTCTCGAAAATAAATAAGACATCCTACAGATCCTGAATACGAGTGATATACAGAATATGTTACTGTGTTATATCCCTGATATGTACCCCTTCTGAAGAGATTAGCAATCTCTTCCAAAATGAGTGAATTGCAGACACTCATCTAATATCAAATAAATAGGCAGATCTCTGATCTCACTCCCCTCCCCACACCATAGAAAAATGGTCTTACGGTTATATGAATTCAGAACATGAGGGAATCGCATCCTGGCTCTACCCCTCTGTCCTATGACTCCCAGGACATACTATCCTGCTATTTATACTTAGGTGACCCATATGTTTGAGGTTTCTGAAATATATATTGGCATTTGTACATGTTCAAGAGAATAAGCCAGGTATAGTTTAATGCTGCACCTTAAGAGACCTTTTTAATCAACTTCTAGAACAAAGGGTAGAGCTCTGTTTCCGTGTGAAGGAAATAAGAAAATGCATGTCAAAAAGTAAAGGTACGGTTCATAAAAGAAAGGTACAAGAAGTGCTCCTGTCCAGGACACTCTGCGTAAAATAAATAGATTAATTACCTTGTGTGCCTTTCCCCTTGAAGAGTAGTACTCAACAACAGGAAGATTCAAACTCTCGAACACTTTTAGACGCTTCTTGATTGTCTCAATGTTATCATCAACTCGTCCCTAAGACGCATACATGAGATAATCGAAAAAAACAGAAACAAATAGAGAGATCAATTTAATGTGATATATTTTATTTATTATTTTTAAAGGAAACTCCACTAGAGCATTAATGAATCTCCCATTATGATATGCACGAGCTACACTGATGAGATTAGTGTTTCTTCTCTTCAATTTAAATGACATATGAAGAAGAAAGAGCTACACTGATGAGATTAGTGTTTCTTCTCTTCAATTTAGATGACATATGAAGAAGAAAATCATTTGTGGCAATCCACAAGGAATATCCTTTAGGAATGTTAATATTTCATGTTAGTTAAGAGAGAGGGAGTAATAAGAAGATAATTGTCAAAGCCGAAAGTAACGGCTGGGAAATAAAATCTTAGAAAAACACATATCAAGTAAGTTCCGAATAAGAATTGAAAAATGTACAGTACTTATATAGTGTATAAATAGCACAATTTACTCCAGAGATAGTACAAAGACCAACCTGGTTACGGCTTAGGAGACGTTTAACCATCTCATCCTCAGGACAGTCAAAGAAAAGCACAAGGTCTGGTTCAGTTCCAGTCTGAAATTTATTAGTCAACATATCAGAACAGGACCATGCAATCTACATTTATCTGACTAACAATTATTGAGTGGACAACAATGAAACATATCTTAGTTGGTATGCCAGTGTCTTCTTATTGCCCGACCACAGAACACACATTTTCTTTGATATAAATGCTTGTATTAGTTGTTGAGATGCAACCATACATCCACCATAGTGGGGCTAACATAACTGTGGACTACTGCATAAAACCTAAAAGTGCATCACTCTAGAGCAGGACATAGTTATTGATTTGCTACTAAAAAAAATACATACATAAGAAACTGCTGATTGCATACCGTACACTGGCAGTTTTGGGCCAGCATATTGGCATAAAAAATGTAAAGACAAGTGTGTATACACAAATGTTACAGTCACAAGAGCTACAAAACTTGAAGATTCACCTGTTATCAGCCTTCCCGAAAACTGAACAGAAACTGTTGAACATCATGGACTTATCCATCTCATCACATACTATTTCAATTAATAGAAAGTTCTGGACCAGTAATGCAAGCCAAACAGAAAACTTACTATTTTTTCAAAGGCAATTCTGTTTTCCTCACACCTTGGGAAACCATCAATAAGAACCCTTTTAGCAGAGCTTGATTCAATTGCTTTTCTGACAAGCTCAACAGTTATCTCTGATGGAACAATCCTCCCTTCCCTTATTATCTCTAGGATCCACTCCCTGGCAAATGCCATGGCACTGACATATCAATTGACCGAACCATGCATGTGCGTGTGGGTCATTATAAGGCTATATCAGAGGATTGAACGGAAAGAGGTATTACCCATGATCAGTGCCCGAAGATATTTCATTGCGCAGAAGGTCACCAGCACTCACATGAGAAAATCCAAAATCCGAAGCGATCCTACTACATTGCGTTCCTTTCCCACTCCCAGGTCCCCCTACATGCAGGATCTTGATTAGATCATGTGAACATTTGAAATTTGAGCGCCACAACACAATCCAACTTGAAACAGCACTTATTGTCATGGGAGGGAAGGGATCCACAGTTTACTCTACACAGGATATGCATGGAATTTATAAATGTGAAACCAACCCTGCAACATTCTGGGAGTAAAAGCAGGGAAGAATTACCTAAGACGAAAGCGATGAACGGCCTGTCACCATCGCTTCCCTACAGAATGAACAGACGGCACATCGCCAAATTATTCACAAGAACTCTGCAAGATTTGTCACAAATAATAAGAATGCAGGCTTGGGAACGAACGTACAGCTAGGGACGTGAAGAGCCTGAGCAGATTCGGGGCCGGCGGTTTCTGCGAAGAGAACAGTCAGGCGGATGAGCATCTAGGAGCGAGAGGAACGTATTGACCCCCAATGGAGCGAGAGAGGCGAGCACGCCAGATCCGGTCCGGCCACGTTGTGCGCGCGTGCGAGGCGTTCGACGAAATGCGCAGGAGGAAAAGGAAAGGGGAACCGTGGGGTTACCTCGGCGTGGGTGGGGAGGAGGAGGTGGTGCTGCTGGTGGTGGCAGGAGGAAGCGACGGCGGAGGAGCACGGGGATCGGAGGAGAAGGGAGCTCAGCCGCCGCCTCAGCATCCCGACGCCGATCGCTCCCGCCTTGCTTGCCGTGCAGGGCAGGACAGAGGAGAAGCAAGGGGGGCTCGCCGTTCACCCCAGCCGCGGGAGGGGCCGAACGGCCGAGGTCGGCGGAGGGAGGGGCGGCGGCGAGCGGCCGTCGACTTGGCGTGGCGGGGAGCGGGGCCGGGACGGGGAGAGTTCGTCGTTTCTTTTCCTTTTCGGAGCTGCTTCTAATCGATGAAAGTGAAAAAAAAATGCCTTTTCCGCTTCGCGTGTCAGGCAGCGTGGAATATCTTGTGCTTTTAGCAAGGGGAAGCATGATGAATTGTCAAAAGCATAAATTGTTAGTATTTTTTATGGGTTGCGATAACTGCCACACGTGTGGCATGGATACGAACCCGCCCACACGCCTCGTGTGGCGTGAACAGGAACCCGCCCGCACGACCGCGTCCGGGCGCGCAATTGCACAGCCCGCACGTCCGGTGTGTGGCAACCCCGTCCGCACTGCTCCATCCGGGCCACACGACCCGCTGCCCGCACGACCCAGCCGTTCCCGTCCTCGCTCGATCGCCTCTCTCGACTGCCGCCATCGTCCCCCACCTCTCAAACACCTACCTCCGTCGCCGGCCTTCTCTGGTTGCCATGGAAACCAAAGCAGATTCGTAGGGCAAACCCACCGCAAACCACACCCCAACCCTCCCCAGCCCCAGCCCCCCACTCCAATCCAGCCCTTCAGAGACGATCAGCTAAAATCAGGTGAAAAATCTCCCGATCTCATCCTTCTCATCCTTCAGGCTGAAAATCTCCCCGATCTTGTACTGGATCCATGCTATAGGGGGCTGGCAACACCAGTAGTTCTGAGATCCAGTAGTTTTGTGATCTACGTGTTGATATTAACTTAAGTTCCTAGTTTGATTGACGCAAGTAGTTGGGTAGGCAAATGGATGAGTAGGAATCCCTGAAAAAAGGAGATGAAGGACAATTTTGGAATAAAGGGGGTGGGAAAAATTAATTGCCACTTGTGTACAACGATAGTTGCCACCTCTCGTTGTCAGTAGCTGCCACCTAGTTGACCTGTTGCTTGCCATCCCTATCTTCTCTGTGCAAGCAGCAGAAGAATACAATTCTTGTGGATTATTGATGCGTTCTTGATCATGCAGTGATTGAGAACATATTGGAAAGAAGCGAGCCACGGAAAGAATGAATCACGAAGATGGAGCTAATACTTGGGTTCTCAAAGGCCAGAAACGCCCCCTTGGGATGCATCAGAGTACAGTCAACTCATCTCTGCAGGGCCTTTGCTGCCACTACTAGAACAGTATACATGCATTGGTATGATGCGTGATAACAAAAATAAGAGAACAGTTGCCATCTTCGTAACGATGAAGATGACATTCTACTTATGTCATGCACTTTCATTTTTTCACAGGTTCTTATGACCAAAACACTCTCAGGGGCCCCCATGGCAAGTTCAGTTACAAGGCGCTAACACTAACAAATGTACGGGCAGCCAACTTCAACTAGCTAATGTGGCTAATCAAGGTAATGCATACGAAAAAAGAGCACATACTGGTGTGGACATGAAAAATGAACATGCAAAAAAATGGCAAAAGGACTCACGAGTTATCACGGGTGAAAAATGCAGAGCCTTCATGCAGCACGTGGCATCAGGCAGCACCCATGTACCTGCCATCGACGTCATACACAGGTGAGTCCATAAAAAGTGAAGTTGCGGATGCTCAATTAGCAGAAGGAACATGGTTGACAATTACACTTGCCATGACTGGACATCTACAATTGCCATGGTTAAATAACTGCAGTTGTCATCCCTGGACAAATGCTGTTGCCATCCATGGACAACTGCAGTTTCCATGGAGGAAGAACTGCAGTTGCCATGCTAGTGTAACTGCAGTTGCCATGCCATGACAACTTCAGTTGCCATGGAGGAACAACTGTAGTTGCCATACGAGTGCAACTACAGTTGCCAAGCCAGGAAAACTGCAGTTGCCACATCAGGGGCAACTAGAGTTTCCGTGCTAGCACAAACTGCATTTTCCATGAATTGACCAACCACTACTATGCTTACAGGTTATTACGGTGGTGGTAACCGGGCAAACGTCTCGTGGCAAGATTCACAAATTGCAGGTGGAGCACGTCATTTTGGTGTCACAGACCACACAAGATGGTCACATGCAGCACAACAAGGTAAAGAAAAAAACTGAACCACAAAACAGCACTACATTTGTTTTGGTGGCAAAAAAAGCACGTGCCGAAAAAAAGAATTGAAGCAGTGATGGTGGACAAGAACGAAGCATAAAACACTGAAAAAGTGGTTGCGTGCAAATGAGTCATGTATTGTCTTTGAAAAATTGCCAACTGCTAAAAGCATGTACGCATCACTAATGGTTGAACACATTTCGCCTAGTGAGATCAATCTTATAATGCAGTAGTTTTGTTGCGAGAACACAGTGGTTGCCATTTGTTGGTTGCAGTGTTTGCCATGAATGCAGGACTGCAGCTGCCATGCATATAGAACCCAAGTTTTCCTACTAAAAAAAGGAGCGTGTTGTCGTGCATTGGCAGACATTGGTTGCCATGTTTGTTGAACTGTACCTGCCATGAATGGACAGCTACATTTGCCACACATGTCCACGCGCGAACTGACATCTTGATGTTCGAATGATGTAATGCCAAATCACTCGATGTTAATGTCATCTGTTGCGATAAACATGTTATTCAAACAAAAAAATCTTACTCTCGTACCTGTTTTTTCCATTACAGGGCCTACAACTACACTGAACAGCGGCACGGGCACATCTACTGCGGGAAGCTCTGAGCGCACAGAAGACGCGTTCCCCCAGCCAGCAAACAATCATGCCGCAAATAATGCCGAGTCAGTGTCAGAAATGGCAATCATTCCAGCAATTCCCACAACCACCCCTTTGAACGCAAGCACTGGCAACATTCAAATCGATGAAACTGCTGCCGATACTGAAGTGAATGATGAAACTGACGACGAGGCACAAGGGGATGAAGATGATGGGCAACCAGATATCATGGTACCTCTGCCACCATACGTTGGGCAAAGATTTGAATCGTTTGAAGAAGCAAAGGAATTCTACCAGACATATGCAAAGTTTCATGGGTTTGCCGTCAACACCGAATACCATAGAAAAATTAAGAAAACTAATGAGTACAACAGAGGTGAGATGTGGTGCCACAAGGTACGAAGGAACAAGAAAGGGAAAGGTGTTGCGCCTGTCGTTCCGAAACGAAAGAGAGGGATCATTCTCAAGACGGGTTGCCCTGTCCGGTGTAAGCTAAACATAGATGGAACACAATATGTGGTCACTGAGTATTTTGACGAGCACAACCACAAACTCATAAAGAAGTTTGATCTGGTCAAATTTCTGACCGCCCACAGAGGGTTCACCCCAGTCGAAAGGCAATTCGTAAAGCTGCTACATGATTGTAACATCGGTCCATCAAGAATGGTCCAGATACTATCACTCATCCAGCAAAAAGGGGAAACTGAGTAGCATGCCGTACATACCAGCTGACGTCACAAACCTACAGGCCAAGTACCATAGAGAGAGCAAG
5
+ >2BS_5163353 11974 190831 503566-,...,5145698-
6
+ ATGTAGTGGTGGTGTGTGAGCGACATTTGAATAGTTTCTGTAATATATGTGTGGATGTGAACCTACTTAGGCGTGACAGCAGATATGTTTTTTATATTTATCATTATTACTGTGGTTTCATCGTTTGTATCACCCGTTTGCTGTTTTGAATGCATCACGATGTTTTAAAACATGGCAAATGTAGTTCATCAGACATGGTTAGCGAAAGTCATCGTCATTGTTCATTTGGACATTTTGCTTCTCCTATTTCATCACTAACTTCACCGGCTAGCATGGTAGGTTGATCATGGAGCCACAACCTTTGATGCGGTTTATGCACATTACGCTTTTTCTAACTTGTTTCCCATATATTGCACACAACACAATATGTGTCACTAAACCGCGTCGATTTTGTCATATAAATATATGCGATGCAGAGTCATTACAAATACCATGGCATATTTTCAGTACAGCTAACATGGCAGATCCAGTACAAACAACACGGCAGATCCAGCATAATTAACATGGCAGATCCAGCATAACTAACATGGCAAATCCAGTACAACTAACATGGCAGATCCAGTGCAACTAGCATTGCATATTTTTCAGTATAAAATAGTATGGCATATTTTTAGTACAAAATAGCATGGCATATTTTTAGTATAAAATAGCATGGCAGATTAACTACATATAACATGGCAGATTAAGTACCCATACCATGGCAAATTAAGTACCCATAACATGGCAGATTAAGTACCAACAATATGGCAACTACATTTACCCATTCAATGCATTCTCCTTCAACTTCTGAAGCCCCTCTAGAGTCATTATCCCAAATCAAAAAAGTTCACAGCATATAGGGTACAAAACAAATGTCCACAATGACCATGTCACAGTGCCCCAACTTTTGCTTACGGATTGCCCGTCCCTTTCTTCGTTTTCTTGTGTTTTGCTTGAATCTCCAATCCCGATTTTTACCTTATCTCTCTTGGACGACCCTTTGTGATGGAACGGGGTGGGTTCCTGACCTGGGTTTGTGTGCTTGCTGTTCCAGATCCTATCTCAGAGTTTTCAAATGATGGCCCCATGGATGAAGGCACACTTGATGTGCGGGGGAACCGGTGTAAGGCTTCCTCAGCTTTCCTCTTCTTGATCTCATCAAGCTCTCTTTTCAGGGCCTTCCTGTGTTTTTCTGCGACTCTCGTTGTCTCATCACTCTTGCACGCTTCATCAATTAGCTCAGCATAGTTCTTTGTCAACACAACATGCCTCACGGCTTCCATGGTTGCCTCAGGTCTCTCGTCATGCACAGCCAGAACTGCATTTGTTGTCTGCGGCGCAAACGCGTCGTCAGCGTCCCAAGTCCATCGCCACCTTATGAAATGGCGGGGCATGCGTGTCACAGCGTTCTCCATTACTTTCAGTATGTGACAACACACAATGTCGTCCCGTTCAAACTTGCAGCATTGGCAGTAGTATTGCCTTGTTCGATTGAGGCTTTCACGAAGTAGTTCCTTCCTTTGTCCGGGTCTTCAGGTTCTGAATCCGACATGCCCAAAATAGAACACACCTTGAACGTATGTTCGTCCGCCTGGAAAGCCGTGAACATGCTGGCACGCTTTATTTCTTCCTTGAATCTGCAAGTTTTGTGTGTTTTCTATTAAACTATCGTGTGATCTTTTTTTGTAGCATCTTATGTTGTCATGGAAATAGAAATAGATTTTTGTTTGAACATGGCAAACATAGTATACTAAACATGGCAAACATAGTGCACTAAACATGGCAAACATAGTACACTGAACATGGCAACTGCAGTAAACTATACAAGGCAACTGTATTGCATTTTCAACTGACCATTGTCATTTCCACACCAACACTCCTCACTGGAAGCCCTTTGCATAAAACATGGCAACTGAATTTTTATTGAACATGGCATCTACAGTTGAGTAAACATGGCAACTGCATTTTTCAACAAACATGGAAGTTTTGCATTTTTAGAAAACATGGCAACTGCATTTCATCAAATGTGTCTAGAATATAACGTTTTTCAGTTGACATTGGCATTTGCATACCAACATGCCCCAATGGCAGTACAGTGCATTAAACATGGCAACTGCACTTCATTAAACATGACAAACAAATTTTTCATTAAACATGGCAACTGCAATTGAGTAAGCATGGCAAACAGTATTTCATTACACATGGCAACTGCATATCATGGCAACTGCATTGCACCCTATAAGGCACCTACTGCCTTAGTGCTTTTTTTGCAAATAAGACTGTAATGCAACTGACTTACTTGTTAAAGATCTTGTTTGTGTATATCTTGCTCATTTGCCTCTCCATCGGTAAATACGTTAGCAATTTTGGCTGCTTTAGCGCGGTGGTAGCTTCTTGCTGCAGCTCAGATCCCAGAATTTTTTGTTGCAAAGCTGTGTACTGCTTGACAAACTGTAGCAATGAGTTGCCAGGGCTCACGTACCGCTTCAAAACAGCATTGAACCCCTCGCTGCACTGCGTAGTCTGCAGAAAGGGAAAGAAGCACTGCATGAAGTAGACTGGCACCCAGTACATTCGCTTCTCCCACAGGTTAGCAAGCATCTTGTTGTCTTGGACTTGATATGTTTCAATCATGGCCATCCAGCTCCTTTCAAACTCCTCCACCGTCAAGTTGTGGTCCATGCACAGCTCGAACGCCTTGTGGAGCTCTGGACGGTCAGCAAAGAACGGTCCTAGCGTCTCTTCAGCCCTCTTTATAATGTGCCACCTGCAGTGCCTGTGCACTGCCAACGGAAATACCTCCTATATGCCTGCACGCATGCTGAAATCCTGGTCTGTTATTATGTTCATCGGAGCAAGTCCATCCATGCACTCCAAGAAGGTCTTGAACAACCAAACGTACCCATCCGTGTCTTCGTTCCGAACGAGCCCGCAGCCGAACTGCAATGACTGATTGTGGTTATTTATTCCTATGAACGGAGCGCATGGCATCTTGTACATATTAGTCAGATACATCGCGTCGAATGAAATGCAATCTCGAAAATGTTTGTAGGCTCTCCTTGCAGCACCATCCACCCAATACATGTTCCTGACACGGTCCTCATCGTCCAATCTTATCCTGTAGAAGAAATCTGGATCTTCCTTCGCTTTCTCATCGAAGTAGGCCATTGTGACCTCTATGTCAGCAAGCTTGCTCTCTCTATGGTACTTGGCCTGTAGGTTTGTGACGTCAGCTGGTATGTACGGCATGCTACTCAGTTTCCCCTTTTTGCTGGATGAGTGATAGTATCTGGACCATTCTTGATGGACCGATGTTACAATCATGTAGCAGCTTTACGAATTGCCTTTCGACTGGGGTGAACCCTCTGTGGGCGGTCAGAAATTTGACCAGATCAAACTTCTTTATGAGTTTGTGGTTGTGCTCGTCAAAATACTCAGTGACCACATATTGTGTTCCATCTATGTTTAGCTTACACCGGACAGGGCAACCCGTCTTGAGAATGATCCCTCTCTTTCGTTTCGGAACGACAGGCGCAACACCTTTCCCTTTCTTGTTCCTTCGTACCTTGTGGCACCACATCTCACCTCTGTTGTACTCATTAGTTTTCTTAATTTTTCTATGGTATTCGGTGTTGACGGCAAACCCATGAAACTTTGCATATGTCTGGTAGAATTCCTTTGCTTCTGCAAACGATTCAAATCTTTGCCCAACATATGGTGGCTGAGGTACCATGATATCTGGTTGCCCATCATCTTCATCCCCTTATGCCTCGTCGTCAGTTTCATCATTCATTTCAGTATCGGCAGCAGTTTCATCGATTCAAAGGGGTGGCTGTGGGAATTGCTGGAATGATTGCCATTTCTGACACTGACTCGGCATTATTTGCGGCATGATTGTCTGCTGGCTGGGGGAACGCGTCTTCTGTGCGCTCAGAGCTTCCCGCAGTAGATGTGCCCGCGCCGCTGTTCACTGTAGTTGTAGGCCCTGTAATGGAAAAAACAGGTACGGGAGTAAGATTTTTTGTTTGAATAACATGTTTATCGCAACGGATGACATTAACGTCGAGTGATTTGGCATTACATCATTCGAATAGCAAGATGTCAGTTTGCGCGTGGACATGTGTAGCAAATGTAGCTGTCTAGTCATGGCAGTTACAGTTCAACAAACATGGCAACCACTGTTTGCCAATGCACGACAACAAGCTCCTTTTTTTAGTACGAAGACTTGGGTTCTATATGCATGGCAGCTGCAGTCCTGCATTCATGCAAACACTGCTACCAACAAATGGCAATCACTGTGTTCTAGCAACAAAACTACTGCATTATAAGATTGATCTCACTAGGCGAAATGTGTTCAACCATTAGTGATGCATACATGCTTTTAGCAGTTGGCAATTTTTCAAAGACAATACATGACTCATTTTGCACGCAACCACTTTTCAGTGTTTTATGCTTCGTTCTTGTCCACCATCACTGCTTCAATTCTTTTTTTGGCACGTGCTTTTTTTTGCCACCAAAACAAATGTAGTGCTGTTTTGTGGTTCAGTTTTTTTCCTTACCTTGTTGTGCTGCATGTGACCATCTTGTGTGGTCTGTGACACCAAAATGACGTGCTCCACCTGCAATTTGTGAATCTTGCCACGAGACGTTTGCCCGGTTACCACCACTGTAATAACGTGTAAGCATAGTAGTGGTTGGTCAATTCATGGCAAATGCAGTTTGTGCTAGCACGGGAACTGTAGTTGCCCCTGATGTGGCAACTGCAATTTTCCTGGCATGGCAACTGTAGTTGCATTGGTATGGCAACTGCAGTTGTTCCTCCATGGCAACTACAATTGTCATGGCATAACAACTGCAGTTACACTGGCATGGCAACTGCAGTTCTTCCTCCGTGGCAACTGCGGTTGTCCATAGATGGCAACAACAGTTGTCCAGGGATGACAACTGCAGTTATTTAACCATGTCAATTGTAGATGTCCAGTCATGGAAAGTGCAATTGTCAACCATGCTCCTTCTGCTAATTGAGCATCCGCAACTTCACTTTTTTATGGACTCACCTGTGTATGACGTCGATGGCAGGTACATGGGTGCTGCCTGATGCCACGTGCTGCATGAAGGCTCTGCATTTTTCACCCCTGATAACTCGTGAGTCCTTTTGCCATTTTTTTGCATGTTCATTTTTCATGTCCACACCAATATGTGCTCTTTTTTCGTATGCATTACCTTGATTAGCCATATTAGCTAGTTGAAGTTGGCTACCCGTACATTTGTCAGTGTTAGCGCCTTGTGACTGAACTTGCCACGGGGGCCCCCTGAGAGTGTTTTGGTCATAAGAACCTGCGAAAAAAATGACAATGCATGACATAAGTAGAATGTCATCTTCATCGTTACGAAGATGGCAACTGTTCTCTTCTTTTTGTTATCACGCGTCATACCAATGTCTGCATATTGTTCTAGTAGTGGCAGCAACGGCCCTGCAGAGATGAGTTGACTGTACTCTGATGCATCCCAAGGGGGCGTTTCTGGCCTTTGAGAACCCCAAGTATCAGCGCCATCTTTGTGATTCATTCTTTCCGTGTCTCACTTCTTTCCAATATGTTCTCAATCACTGCATGATCAAGAACGCATCAACAATCCACAAGAATTGTATTCTTCTGCTGCTTGCAGATAGGGATGGCAAGCAACAGGTCAACTAGGTGGCAGCTATTGACAACGAAAGGTGGCAACTGTCGTTGTACACAAGTGGCAATTAATTTTTCCCACCCCCTTTATTCCAAAATTGTCCTTCCTCTCCTTTTTTAGGGATTCCTACTCATCCATTTGCCTACCCAACTACTTGTGTCAATCAAACTAGGAACTTAAGTTAATCTCAACACGTAGATCACAAAACTACTGGATCTCAGAACTACTGGTGTTGCCACCCCCTATAGCATGGATCCAGTACAAGATCGGGGAGATTTTCAGCCTGAAGGATGAGAAGGATGAGATCGGGAGATTTTTCACCTGATTTTAGCTGATCGTCTCTGAAGGGCTGGATTGGAGTGGGGGGCTGGGGCTGGGGAGGGTTGGGGTGTGGTTTGCGGTGGGTTTGCCCTACGAATCTGCTTTGGTTTCCATGGCAACCAGAGAAGGCCGGCGACGGAGGTAGGTGTTTGAGAGGTGGGGGACGATGGCGGCAGTCGAGAGAGGCGATCGAGCGAGGACGGGAACGGCTGGGTCGTGCGGGCAGCGGGTCGTGTGGCCCGGATGGAGCAGTGCGGACGGGGTTGCCACACACCGGACGTGCGGGCTGTGCAATTGCGCGCCCGGACGCGGTCGTGCGGGCGGGTTCCTGTTCACGCCACACGAGGCGTGTGGGCGGGTTCGTATCCATGCCACACGTGTGGCAGTTATCGCAACCCATAAAAAATACTAACAATTTATGCTTTTGACAATTCATCATGCTTCCCCTTGCTAAAAGCACAAGATATTCCACGCTGCCTGACACGCGAAGCGGAAAAGGCATTTTTTTTTCACTTTCATCGATTAGAAGCAGCTCCGAAAAGGAAAAGAAACGACGAACTCTCCCCGTCCCGGCCCCGCTCCCCGCCACGCCAAGTCGACGGCCGCTCGCCGCCGCCCCTCCCTCCGCCGACCTCGGCCGTTCGGCCCCTCCCGCGGCTGGGGTGAACGGCGAGCCCCCCTTGCTTCTCCTCTGTCCTGCCCTGCACGGCAAGCAAGGCGGGAGCGATCGGCGTCGGGATGCTGAGGCGGCGGCTGAGCTCCCTTCTCCTCCGATCCCCGTGCTCCTCCGCCGTCGCTTCCTCCTGCCACCACCAGCAGCACCACCTCCTCCTCCCCACCCACGCCGAGGTAACCCCACGGTTCCCCTTTCCTTTTCCTCCTGCGCATTTCGTCGAACGCCTCGCACGCGCGCACAACGTGGCCGGACCGGATCTGGCGTGCTCGCCTCTCTCGCTCCATTGGGGGTCAATACGTTCCTCTCGCTCCTAGATGCTCATCCGCCTGACTGTTCTCTTCGCAGAAACCGCCGGCCCCGAATCTGCTCAGGCTCTTCACGTCCCTAGCTGTACGTTCGTTCCCAAGCCTGCATTCTTATTATTTGTGACAAATCTTGCAGAGTTCTTGTGAATAATTTGGCGATGTGCCGTCTGTTCATTCTGTAGGGAAGCGATGGTGACAGGCCGTTCATCGCTTTCGTCTTAGGTAATTCTTCCCTGCTTTTACTCCCAGAATGTTGCAGGGTTGGTTTCACATTTATAAATTCCATGCATATCCTGTGTAGAGTAAACTGTGGATCCCTTCCCTCCCATGACAATAAGTGCTGTTTCAAGTTGGATTGTGTTGTGGCGCTCAAATTTCAAATGTTCACATGATCTAATCAAGATCCTGCATGTAGGGGGACCTGGGAGTGGGAAAGGAACGCAATGTAGTAGGATCGCTTCGGATTTTGGATTTTCTCATGTGAGTGCTGGTGACCTTCTGCGCAATGAAATATCTTCGGGCACTGATCATGGGTAATACCTCTTTCCGTTCAATCCTCTGATATAGCCTTATAATGACCCACACGCACATGCATGGTTCGGTCAATTGATATGTCAGTGCCATGGCATTTGCCAGGGAGTGGATCCTAGAGATAATAAGGGAAGGGAGGATTGTTCCATCAGAGATAACTGTTGAGCTTGTCAGAAAAGCAATTGAATCAAGCTCTGCTAAAAGGGTTCTTATTGATGGTTTCCCAAGGTGTGAGGAAAACAGAATTGCCTTTGAAAAAATAGTAAGTTTTCTGTTTGGCTTGCATTACTGGTCCAGAACTTTCTATTAATTGAAATAGTATGTGATGAGATGGATAAGTCCATGATGTTCAACAGTTTCTGTTCAGTTTTCGGGAAGGCTGATAACAGGTGAATCTTCAAGTTTTGTAGCTCTTGTGACTGTAACATTTGTGTATACACACTTGTCTTTACATTTTTTATGCCAATATGCTGGCCCAAAACTGCCAGTGTACGGTATGCAATCAGCAGTTTCTTATGTATGTATTTTTTTTAGTAGCAAATCAATAACTATGTCCTGCTCTAGAGTGATGCACTTTTAGGTTTTATGCAGTAGTCCACAGTTATGTTAGCCCCACTATGGTGGATGTATGGTTGCATCTCAACAACTAATACAAGCATTTATATCAAAGAAAATGTGTGTTCTGTGGTCGGGCAATAAGAAGACACTGGCATACCAACTAAGATATGTTTCATTGTTGTCCACTCAATAATTGTTAGTCAGATAAATGTAGATTGCATGGTCCTGTTCTGATATGTTGACTAATAAATTTCAGACTGGAACTGAACCAGACCTTGTGCTTTTCTTTGACTGTCCTGAGGATGAGATGGTTAAACGTCTCCTAAGCCGTAACCAGGTTGGTCTTTGTACTATCTCTGGAGTAAATTGTGCTATTTATACACTATATAAGTACTGTACATTTTTCAATTCTTATTCGGAACTTACTTGATATGTGTTTTTCTAAGATTTTATTTCCCAGCCGTTACTTTCGGCTTTGACAATTATCTTCTTATTACTCCCTCTCTCTTAACTAACATGAAATATTAACATTCCTAAAGGATATTCCTTGTGGATTGCCACAAATGATTTTCTTCTTCATATGTCATCTAAATTGAAGAGAAGAAACACTAATCTCATCAGTGTAGCTCTTTCTTCTTCATATGTCATTTAAATTGAAGAGAAGAAACACTAATCTCATCAGTGTAGCTCGTGCATATCATAATGGGAGATTCATTAATGCTCTAGTGGAGTTTCCTTTAAAAATAATAAATAAAATATATCACATTAAATTGATCTCTCTATTTGTTTCTGTTTTTTTCGATTATCTCATGTATGCGTCTTAGGGACGAGTTGATGATAACATTGAGACAATCAAGAAGCGTCTAAAAGTGTTCGAGAGTTTGAATCTTCCTGTTGTTGAGTACTACTCTTCAAGGGGAAAGGCACACAAGGTAATTAATCTATTTATTTTACGCAGAGTGTCCTGGACAGGAGCACTTCTTGTACCTTTCTTTTATGAACCGTACCTTTACTTTTTGACATGCATTTTCTTATTTCCTTCACACGGAAACAGAGCTCTACCCTTTGTTCTAGAAGTTGATTAAAAAGGTCTCTTAAGGTGCAGCATTAAACTATACCTGGCTTATTCTCTTGAACATGTACAAATGCCAATATATATTTCAGAAACCTCAAACATATGGGTCACCTAAGTATAAATAGCAGGATAGTATGTCCTGGGAGTCATAGGACAGAGGGGTAGAGCCAGGATGCGATTCCCTCATGTTCTGAATTCATATAACCGTAAGACCATTTTTCTATGGTGTGGGGAGGGGAGTGAGATCAGAGATCTGCCTATTTATTTGATATTAGATGAGTGTCTGCAATTCACTCATTTTGGAAGAGATTGCTAATCTCTTCAGAAGGGGTACATATCAGGGATATAACACAGTAACATATTCTGTATATCACTCGTATTCAGGATCTGTAGGATGTCTTATTTATTTTCGAGACTTGACCTTTAGGGTGTCTAAGAGTACTTGTTGAACTCCATGCAGATAAACGCAACGGGAACTGAAGATGAAATCTTTGAAGCAGTTCGCAAGTTGTTTTCCACCTTAAGGTAAATCCTGATGTTCAAGACACGGAATTCCTCTCATATACTGTGACTATGTCCGCTCTCCTGAAGGAGGTGCACATTCTTGCATCATCTGATTTCTGATTGCTTGCATATCATTTTCTAGTGCCAAATTAGTTCTGAAAGTTTTCAGTCTTCACATATTTAGCATATCCTAACCCTTCCAATTAAATGCCTAAGCTGCAGCACCCACACTTTTCAATACATTAACAAGTGATGACGAGTTCTTTATACCAAGCCTGCCATTGCAGCTAGGGCAGGCCGTCGCTATAAGCAAAAGGATATTGCTTGTAGGTAGAAAGAGACTGAATTTCTGATCCTTGCCATTTGTGTTCATTGTGCACAATAAAGAAAGACAAACTGGCAAGCAAAATGAATGAACAAAGTAGCATCTGCCTTGTTGATTGATGGATAAGGAAGAAGAGGTCCAGGGGGTTGCAACGTAGCTAACTTTGTCATCTAGCTGACTCGCCGAACCCTTTCCATGTAGCTAGTTTCTCGACACGTTTGACTAAGCAATACCGGCATCTTCTTCTATCTAATATAATAAGTAAAAGAGAGTTGCAATGATTTGACCGTTTCGCCCTGTTTGCAGGCTATGAGGAGGCTGTCGTCGTCTTCCGCAGGAGCCGGGGGAATCAGAGTTACGCCTGGCGGAAATTCTCTTCCTTTCCTTATTTACTTTTACATGTCTTGGCATAATAATTGTACATATTCTGATACACGGTCCATGCCTGTGAAAACAGAGGGCCCCCGGTGAAACGCTCAGCCCGCTATGATAAACCCTAACCCTCACGCTCCCGTCGCGCGCCACGGTATAGCGCATGATACGCTGGTATGGCAGCAATACTGCTACAATGATATTTTTCTTCCAACACCCTAACAATATACTCCCTCCGTCTGGAAATACTTGTCATCAAAATGGGACATATTTTAGTTCTAGATACATCTCTTTTATCTATTTTGATGACAAGTATTTTTGGACGGAGGGAGTATATGCCTGCCCTGTTGTTGTTTTTTTTACTAGAATATGATATTGTTGTATGTGCGCCGTGCTTGTCTGGTGACAATCTGCTATTTGTATACGAGGTGAGAACTGCAGGCGTTCGATAAACTAGTGATTTGACTCAAGGGAAAAGAGAGTGTACGTTTGGATTTAGTTAGTGTGAACCGCCGGCGTGTTTTTGTCAGTTCTTGACCGGCCGTTTAGGTTTAGCTGATGCGATCTTGAAAGAAAAAAAAAATGTGGAGCGCCGGCGCTTGCACGCCAAGTCTAATCTAAAAACTCCCCGTTTCCAAAAGATTGTCTGTCTGTGCACTCCACGGGAGGAGAAAGCTCTGCTACTATTGACTCATTTTTCTTGCCTGCATCATCGCCTTGCAAGCCATGGAGCGCGGGCACCTGGCACTTGCTACAGAGCCGGCGTGTCGTCATCGTCATCGTCGCGCTGACAGGTGGACTTCTCGGAGGGCCTCTTTGGATCCTAGGAATGGAACACACGAATTCGATTGGAATATATGCAAAGGCAGCACGCATGATTGCGAGTGGAACACGCGAAATTGATATGAACACACACGCGAAGGCAACATAGATGATCGCGAAACGCGGAAAAATCGCAGCGTTATTATTATTTTTTTGGCTAGGTACATAATCCGGGCATGTGAAAACTGTCTCGATCGCACGCACATAATCCGGGGAATGAAGAGGTTGAGGTGAACCTGAAATTTGTGTTGGAATCTAGGCATGGAGAGAAAGATGATTCGTCACGAGCAACGTCGGCATCGACTGCACCAAGCTGCCATGACTCCATGGAGTGAGGTCATGTGAAGACTTTTGGGGAGCAAAACTCCAACCGGGCATGTCATAGCCAGCCAAAAATTTCTCATCTATATCTTTCCATCCCCCCAAAAGAGGGGGAGTTGAAGTGCTTGTCTCCGAAAAAAGATGGCGTTTTGCACATCCACACAATCAAGAAAACATAAAATGTGCCCCCGCGGAAAAAAGACACACATAAATGTGACTATTATTTTCTCCCGCAATATATAATTACATTATAGTCCCTTCGTCCCAGAATAAGTGTCTTGAGCTTAGTACAAATTTATACTAGAGCTAGTACAAAGTTGAGTCACTTATTTTGAGACGGAGGGAGTAGAACCTAGCAGAATGTATGAATGTACATTTTAGGCAAATCTATAGACAGAGATCTTCACACTTGTAATGAAGACTTTTGAAAGGGAAAAATGGGTTCAAACTTGACCAAAACTACATCCGGAACTTCATGAATGTGTGAATTGGAGGCAGTGGCG
7
+ >2DS_5334799 7226 338754 1380402+,...,2126614-
8
+ GCGACACCTGGACCAGCGGCGAGTTGTACCGCAGGCCGGGGCCGGGCGGCGCCTCCGTCCCCTGCTCCTGCTCCTGCGGGGACCCGGCCTCCGGCAGCTCCGTCGACACGGCCATTTCGTCACTCTGAACGGGCGGGCCGGCGGAGGGGAGTGAGGACGGCAGGTGGGTGGGTGGAGACAAATCGGCGGAGACGAAGGCGAGGTGGAAGACGATCGGCGGAGGTGGCGGTATTTATAGATGGGATCTTGGTGGCTCCTCTGTTTGATTCTGTTGCCAAGGGGGAGAAAGAGACGGCGACATTGAGGACGAGCAGAGGGCGACATTTGGAGCATCATTAGTCAGTGATGATTGCATTTAATCAGTATATTTGGGAATTTAATTACTGCCCATTTATCAGTTCAGATATGTACATCGTCTTTAATGTGGATATTTATTTGAGGGAGTAATAAGCAAAGATTGTTACAGAGTTTTTCCTTTTTTTTTTGAAATTTTCGAAAATATGTTCGACGTGGCAAAAAAAAAGACCGTAATAGCTGGCGAACGTTCGTTGTGAGGGGTGGTATTGCGAACATTTTTGCTGACAGTTTTTTCAGAGACACATGACAATTTTTTTAGATACGCATGACGGTTTCTGGAAAGAAGACTGCGGCAAAAACAAGCAGAATTAGCCATCTCCTACCACCTAAAAATGCCTTAAGAAATTGTTCGCTTGTCACCCTCCTCCCAGGAAACGTTCGCTTGTTAGTATTTTTGAAAGAAAAGGGCCCCACTCAAATTTTGGATCTTTTTGTTTCCATTCATTCGTTTTGGAGCGGTGTAGGGGAGACAGACGAATTTTGAAGATAGAATTTGTGACATTTGGTTTGGAGCATCATCATCATCGCCAATTGGAATGCACACTGAATTAAATTTTGCGAGATATGGCATTCTATTTTCATAAGTTCTTATTACAAATCATCATGATCTCCATAACACATACGCGAGTTGGAAGGAGATTTGTGCACACACGAGATGAGCCGACAGAGATGAGAAGGGGTGAAAGATAGGAGAGTCGAATATGCCGATTTGAAGAATCACCAAACTACATATAGTAAACAATTATTAATTTTCATGAGGGATATTCCGGCATTTTAACAATGGTTGCTAGGTACTAAGGATTTCTTTTCTGTGGGACTTGGCAGTCCTTCCACTCAATTTGTTCAACACACAAGTGGGTATATATATGGCAGATACGGTATAATATAAGACACAAAGGCACAGAAGAGGATTTGTGGCTCTCGGGTGCCACGCACCCCTATATGAATATAGCATTAAAAACATATTGCAAATTTTAAAAAATTCTAAAATTTTGGAATATGAACCTGGGTGCCTGTTGTACACTCGTCTTCAACTTCGTGGGGAATGAGTGCCCGTGTTCATCTCAGTAAAAAGGACAAAAAAAATCGTATGTATAGAAAAAGATAATTGGATGTAATGTATGTCAGACCGGAACTCCAGTGCCATGGATACCACGGGCAACCATTCCCAGCGAAAATGAAACAAGTGTGCAATCAATGGTCACCTAGGTTTGATATCCTAAAATATCTGATTTTTGTGAAATTTTCTGATATTTTCTTAATGCTAGTTTCATATAGGGGTACGTGACACTCGGGTGCTCCAATGCATTTCCCACAAGGGCACACGACCTTATCCCTGTCAAAAGACTCATCCTACATTGATAAATATGACTTGGCAGTGGGGCCAGCAACAGCAAGATGAGCGTCCCAACATCCCCTTGTTCTTTCTTTGGAGGATCGATGTTCCTCCATCCAACATTGCTTGGTTGATTGCCACAAGGACGCAGGGCTGAGATTCTCGGTCCTGGGATTTTGGAATTTTGCTCGCAACTTGCAGCGCAGCTGCGTCCCTGTCCTTCCTAGTGAATCACCTTCGCCCGTTGTTTGGGGCATAGGACAAATACACCCAGCTAATCATAGATTCATAGGGAGGGGGTCATAATCCTGGCTAGCTTTCACCGTTAGCGGCAGCTAGCTGGTTAATCATGCAGGCTCACACGAGGCTAGCGGCCCCCTAGTAGAGTCCAAAATGGACCTTTCCTCTGTTTTACCATCTCCGTCTGAGAAAGTGACAAGATGTGGCATCTTAGTAGTTGTTGTTAACTGCAGAATGCTCCCACCATGAAACCTTTGATTTTCATTCTAAAAGGACACCTTCGATTTTCCGAAAAAATGACATTGCGATATTTCCTCCCTTTGCGAAAGTGGGGGCGATTTGGGGATGTGTTCGTAGGATCAGCAGAGTGCGAGTGTCCTCACTACTTAGTGGACTAGGAACACCAATTACCATTCAGCATGGATGGTGGGCTATCTCAAAAACAATGAACAAAGACTAGGAACACCAATTTTTAGGAGTGTCAGCTAGCTGGTTAATCATGCAGGCCAACACGAGGTTAGGAGCTCCCTAGAGTCTAGAATAGATCATTTTCTCCGTTTCACTACCTTTATCTCACAACACAAAACATTTTTAAGAGTCGTAATGATCGGAAGACGAATGCCACACATGACACCCTTGTTTTTCCTTGCGAAATACACATGACATTCGAGCCCCCCTCCAACTCACACATTAATGAAGTTCCAGGTATAGTTTTGGTCAAGTTTGAACCCATTTTTCCCTTTCAAAAGTCTTCATTACACGTGTAAAGATCAGTATCTATAGATTGTCTAAAATATACTCGTAAAGAAATATAAGAGCGTTACAGAGGGAGCACATTCATATATTCTGTTAGGTTTTATAGTTATATATCGTGGTAGAAAGTAATAGTCACATTTATGTGTGTGTTTTTTTGTTGCGGGGGCACATTTATGTTTTCTTGATTGTGTGGATGTGCAAAACACCATCTTTTTTGGAGACAAACACTTCAACTTCCCCTCTTTTTGGGGGATGGAAAGATATAAATGAGAAATTTTTGGCTGGCTATGACATGCCCGGATGAAGGCCCCGTTGGAGTTTTGCTCCTCAAAAGTCTTCACATGACCTCACGCCATGGACTCATGGTAGCTTGGTCCAGTCGATGCCGACGTTGCTCATGATGAATCATCTTTTTCTCCATGCCTAGATTCCAACACAAATTTCAGGTTCACCTCAACCTCTTCATTCCCCGGATTATGTGCGTGCGATCGAGACAGTTTTCACAAAAAAATGTTGCGATATTTCCGCGTATTGCCTTTGCATATATTCCAATCGAATTCATGGGTTCCTTTCCTATGATCCAAGGAGGCCCTCAGGGAAGTCCACCTGTCAGCGCGACGACGACGACGATGATGCGCCGGCTCTGTAGCAAGTGCCAGGTGTCCACGCTCCATGGCTTGCAAGGCGATGATGCAGGCAAGATAAATGAATAGCACAAGTCAACAGTAGCAGAGCTTTCTCCTCCCGTGGAGTGCATAGACAAACAATCTTTTGGAGATGGTGAGTTTTCAGATTAGACTTCGCGTGCAAGCGCCGGCGCTCCACACTTTTGTTTCAAGATCGCATCAGCTACTAAACGGCCGGTCAACAACTGACAAAAACACGCCGGCAGTTCACATTAACTAAATCCAAACGTACACTGCTTTTTTCCTTCAGTCAAATCACTAGTTTATCGGACGCCTGCAGTTCTCACCTCTTATCTGAGAATAGATATATACAAATAGCAAATCGTCACCAGGCAAGCACGGCGCACATACAATTATATCATATTCTGGTAAGAAAAATATCGTCGTAGCAGTTTTGCTGCTGCCATACCAGCGTGTCATGGGTTATACCGTGGCGCACGACGAGAGCGTGAGGGTAAGGGTTTATCATAGCAGGCTGGGCGTTTCACCAGTGGCCCTCTGTTTTCACAGGCATAGACCGTGTATCAGAATATGTACAATTATTGTGCTAAGAGATGTAAAAGTAAAAGGAAAGAAAAGAGTATTTCCGCCAGGCGCAATTCTAATTCCCTCAGGCTCCTGCGGAAGACGACGACAGCCTCCTCATAGCCTGCAAACAGGGCGAAACGGTCAAATGATTGCAACTCTCTTTTGTACAGGTAGTGCATCTTCATTAGAAGAAGATGCTGGTATTGCTTAGTCAAACGTGTCAAGAAACTAGCTACAGGCGAGGGTTCGGCGACTCAGCAAGATGACAAAGTTCGCAACGTAGCATCCTCCCGGACCTCTTCTCCCTTATCCATCAATCAACAAGACAAATGCTACTCTGCTCATTTTGCTTGTCAGTTTGTCTTTCTTTATTGTGCGCAGTGAACACAAATGGCGAAGATTCAGAAATTCAATCCCTTGCCACCTACGTACAAGCAATACCCTTTTGCTTATAAATGTCCTACCCTGGCTGCAACGGCAGGCTTAGTATAAAAGAACTTGCCGTCACTTGTTAATGTATTGAAAAGTGTGGGTGCTGCAACTTGGGCATTTAATTGGAAGGGTTGGGATATGCTAAATATGTGAGGACTGAAAAGCTTTCAGAACTTCAGTAATTGGGACTAGAAAATGATATGCAAGCAATCAGAAATCAGATAATGCAAGAATGTGCACCTCCTTCAGGAGAGCGGACATAGTCACAGAATATGAGAGGAGTCTCGTGTCTTAAGCATCAGGATTTACCTTAAGGAGGAAAACAACTTGCGAACTGCTTCAAAGATTTCATCTTCAGTTCCCGTTGCGTTTATCTGCATGGAGATCAACAAGTAGTCTTAGACACCCTAAAGGTCAAGTCTCGAAAATAAATAAGACATCCTACAGATCCTGAATATGAGTGATATACAGAATATGTTACTATGTTATATCCCTGATATGTACCCCTTCTGAAGAGATTAGCAATCTCTTCCAAGATGAGTGAATTACAGACACTCATCTAATATCAAATAAATAGGCAGATCTCTGATCTCACTCCCCTCCCCACAACATAGAAAAATGGTCTTACGGTTATATGAATTCAGAACACGAGGGAATCGCATCCTGGCTCTGCCTCTCTGTCCTATGATTCCCTGGAAACACTATCCTGCTATTTATACATTGGTAACCCATATGTTTGATGTTTCTGAAATATATATTGGCATTTGTAAAAGTTCAAGAAAATAAGCCAGGTATAGTTTAATGCTGCACATTAAGAAGCCTTTTAAATCAACTTCTAGAATAAAGGGTAGAGCTCTGTTTCCGTGTAAAGGAAATAGGAAAATGCACGTCAAAAAAGTAAAGGTACGGTTCATAAAAGAAAGGTACAAGAAGTGCTCCTGTCCAGGACACTCTGCGTAAAATAAATAGATTAATTACCTTGTGTGCCTTTCCCCTTGAAGAGTAGTACTCAACAACAGGAAGATTCAAACTCTCGAACACTTTTAGACGCTTCTTGATTGTCTCAATGTTATCATCAACTCGTCCCTAAGACGCATGCATGAGATAATCGAAAAAACAGAAACAAATAGAGAGATCAATTTAATGTGATATATTTTATTATTATTTTTAAAGGAAACTCCACTAGAGCATTAATCAACCTCCCATTATGATATGCACGAACTACACTGATGAGATTAGTGTTTCTTCTCTTCAATTTAAATGACATATGAAGAAGAAAATCATTTGTGGCAAACCACAAGGACTATCCTTTAGGAATGCTATATTTCATGTTAGTTAAGAGAGAGGGAGTAATAAGAAGATGATTGTCAAAGCCGAAAGTAACGGCTGGGAAATTAAGTCTTAGAAAAACACATATCGAGTAAGTTTCGAATAAGCATTGGGAAATGTACAATACTTATATAGTGTATATATATCTAGCACAATTTACTCCAGAGATAGTACAACGACCAACCTGGTTACGGCTTAGGAGACGTTTAACCATCTCATCCTCAGGACAGTCAAAGAAAAGCACAAGGTCTGGTTCAGTTCCAGTCTGAAATTTATTCATCAACATATCAGAACAGGACCATGCAATCTACATTTATCTGACTAGCAATTATTGAGCGGACAACAATGAAACATATCTTAGTTGGTATGCCAGTGTCTTCTTATTGCTTGACCACAGCACACACATTTTCTTTGATATAAATGCTTGTAGTAGTTGTTGAGTTGCAACCATACATCCACCATAGTGGGGCTAACATTAACTGTGGACTACTGCATAAAACCTAAAAGTGCATCACTCTAGAGCAGGACATAGTTATTGATTTGCTACTAAAAAAATACATGCATAAGAAACTGCTGATTGCATACTGTACACTGCCAGTTTTGGGCCAGCATATCGACGTAAAAAAAAAGTAAAGACAAGTGTGCATACACAAATGGTTACAGCCAAGAGCGCTACAAAACTTCAAGATTCGCCTGTTATTAGCCTTCCAAAAATTGAACAGAAACTGGTGAACATCATGGACTTATCCATCTCATCACATACCATTTCAATTAATAGAAAGTTCTGGACCAGTAATGCAAGCCAAACAGAGAACTTACTATTTTTTCAAAGGCGATTCTGTTTTCCTCACACCTTGGAAAACCATCGATAAGAACCCGTTTAGCAGTGCTTGATTCAATTGCTTTTCTGACCAGTTCAACGGTTATCTCTGATGGAACAATCCTCCCTTCCCTTATTATCTCCAGGATCCACTCCCTGGCAAATGCCATGAAACTGACAAATCAACTCACCGAACCATGCATGTGCGTGCGCGTCATTATAAGGTTATCAGAGGATTGAACGGAAAGAAGTATTACCCTTGATCAGTGCCAGCAGATATTTCATTCCGCAGAAGGTCACCAGCACTCACATGAGAAAATCCAAAATCCGAAGCGATCCTACTACATTGCGTTCCTTTCCCACTCCCAGGTCCCCCTACATGCAGGATCTTGATTAGTCGTGTGAACAACTGAAATTTGAGCGCCACAACACAATCCAACTTGAAACAGCACTGATTATCATTGATAGAAGTGATCCTCAATTTACTCTACACAGGTTATGCATGGAGTATGTAAATGTGAAACCAACCCTGCATTATTGGGGGAGTGCAGGTAGATTTTTTTTTTACCTAAGACGAAAGCGATTATAAATGTGAAACAGCACTGATTATCATTGAGGCAAGGGTTCCTCAGTTTACTCTACACAGATATGCATGGAATTTATAAATGCAAAACCAATGCTGCAAAATTACCTAAGACAAAAGCGATGAACGGCCTGTCACCATCGGTTCCCTACAGAATGA
data/test/data/BS00068396_51_contigs.fa.fai ADDED
@@ -0,0 +1,4 @@
1
+ 2AS_5222932 6364 46 6364 6365
2
+ 2BS_5245544 8836 6457 8836 8837
3
+ 2BS_5163353 11974 15341 11974 11975
4
+ 2DS_5334799 7226 27363 7226 7227
data/test/data/BS00068396_51_contigs.nhr ADDED
Binary file
data/test/data/BS00068396_51_contigs.nin ADDED
Binary file
data/test/data/BS00068396_51_contigs.nsq ADDED
Binary file
data/test/data/BS00068396_51_exonerate.tab ADDED
@@ -0,0 +1,6 @@
1
+ Command line: [exonerate --showalignment no --bestn 10 --showvulgar no --model affine:local --ryo RESULT:\t%S\t%pi\t%ql\t%tl\t%g\t%V\n /Users/ramirezr/Dropbox/code/bioruby-polyploid-tools/test/data/BS00068396_51.fa /Users/ramirezr/Dropbox/code/bioruby-polyploid-tools/test/data/BS00068396_51_contigs.fa]
2
+ Hostname: [n87398.nbi.ac.uk]
3
+ RESULT: BS00068396_51 0 101 + 2AS_5222932 3015 2914 - 501 99.01 101 6364 . M 101 101
4
+ RESULT: BS00068396_51 0 101 + 2DS_5334799 6812 6913 + 501 99.01 101 7226 . M 101 101
5
+ RESULT: BS00068396_51 0 101 + 2BS_5245544 4549 4651 + 462 96.04 101 8836 . M 69 69 G 0 1 M 32 32
6
+ RESULT: BS00068396_51 0 101 + 2BS_5163353 7425 7323 - 462 96.04 101 11974 . M 69 69 G 0 1 M 32 32
data/test/data/BS00068396_51_for_polymarker.txt ADDED
@@ -0,0 +1 @@
1
+ BS00068396_51,2AS,CGAAGCGATCCTACTACATTGCGTTCCTTTCCCACTCCCAGGTCCCCCTA[T/C]ATGCAGGATCTTGATTAGTCGTGTGAACAACTGAAATTTGAGCGCCACAA
data/test/data/BS00068396_51_genes.txt ADDED
@@ -0,0 +1,14 @@
1
+ 2AS_5222932
2
+ 2BS_5245544
3
+ 2BS_5163353
4
+ 2DS_5334799
5
+
6
+
7
+ for g in 2AS_5222932 2BS_5245544 2BS_5163353 2DS_5334799;
8
+
9
+
10
+ grep -A1 2AS_5222932 /Users/ramirezr/Documents/PHD/201305_Databases/iwgcs/2A*.fa >> contigs_2.fa
11
+ grep -A1 2BS_5245544 /Users/ramirezr/Documents/PHD/201305_Databases/iwgcs/2B*.fa >> contigs_2.fa
12
+ grep -A1 2BS_5163353 /Users/ramirezr/Documents/PHD/201305_Databases/iwgcs/2B*.fa >> contigs_2.fa
13
+ grep -A1 2DS_5334799 /Users/ramirezr/Documents/PHD/201305_Databases/iwgcs/2D*.fa >> contigs_2.fa
14
+ grep -A1 2DS_5334799 /Users/ramirezr/Documents/PHD/201305_Databases/iwgcs/2D*.fa >> contigs_2.fa
data/test/data/IWGSC_CSS_1AL_scaff_1455974.fa ADDED
@@ -0,0 +1,112 @@
1
+ >IWGSC_CSS_1AL_scaff_1455974
2
+ AGGCTTGGAGGCGTCGCTTATGTCGAACTCGATCGTCAGCACCCGCTGGAACTTGGGGAA
3
+ CGTCTTGAACGCCGCAAGCACCGGGGCGTCCTCTGACTGTATGAGCACGCGCTGCTTACA
4
+ GGTCTCCTTGTCGTACCCGGACTTGACAAGCGCTTTGGAGACCGCATCCACCACGTCAAG
5
+ GCTTCTGGCTATAAGGTACGTAGCATGCTGCACTCGGTAGGTACAAGACCTCTCTTGATC
6
+ AGTTGGACAGAATAACAACAATAGGTAACAGGAGCGAGCAAATGAATCATGTCAAAAGTA
7
+ GGATGCTATATATGCTGTCTACCTCTATGCCAATCAGTATACCAGTGATGTTGCTGGCTT
8
+ TTGCAAAATCAAGGAACTCAGCCAGTGTCATAAATTTGCCAGCGTTCTTCGCCGCAGGAT
9
+ TTCTCTCCAGGCGTGCGTCTGCGAATGGACCGACAAGGTTGGCTGCTCCGGTGAACAAAA
10
+ AACATGTGACATCAATGTTAGTGTATGCAAATCTTTGGCAGAACAAAATAAGGAGTATGT
11
+ CTTGCTTCGCACGTTTTAAGGTTTGGATCTCGCTCCATAAGAGATCAAACGAGAAAATGC
12
+ CCGACTTGTTCTGTATCTCATTGACAGTTGTCATTTTTTCCAAGAAAGTTGTGGCTGCTG
13
+ TCGTGCTGCTGTTGAGATCTGTAGAGCCCAGGCAGAAGGCGACCCCGTCTTTCGACATCC
14
+ GGACCCAGCAATCTATTATGTCCGCGCCATCTTTCACCGCTTGTTGGTAGGCAAGGTCCG
15
+ TGCTGTCTGAGAAGACGCCACTCGCACCATTGTGGGTTATGATCAGTGGCCTGACGTTTG
16
+ CTTCTACTCCTGCTTGAGTGGAGGGATCAGTATATTGTGTTACAAGATGATGATAAGTTA
17
+ GCAAAGCAACACCATGGATCAGGAGAAGATTGCCAGATTACCTGGAGGAGCTCCATCTTT
18
+ AGCAATAGGAAAAACGAATGCATTGCCCTTGGTATGTGCCATGCACACTGCAATTAAAGG
19
+ ATCCTCATGAAGATGTTGTCAACAGGAATCAAAAACGAAGAGCTAAATTGTACGGGTACG
20
+ TTCCTATCATGTATAAAGTAACATTTCAAAATTTTCATCAACATCCATTTCAGTTATAGC
21
+ TTCTTCCATTTCAGTTCTTACCTATGGCTCCTGATGCGGTAGGTGGATTGTCTGTTAAAA
22
+ CGCCGTCGACCGAGAAGTCTGAATTGTCTATGAACTGCAAGATTTCCGCGCTGGGATCAT
23
+ AGCTGTAGTTGTAACTCATACAGGGATCATCATTGGCAAATCCAGATGCGTACACTTCCA
24
+ GCCCTAGGGCATGTGCATCTTTGACCAAACTGGTAGACGGCTCCAAGTACATATCCTTAT
25
+ TCTGTGGCCAAATAAATTGCTTGGGAACAAGAATCCCCGATGCAAAGGCCTTGATAGATT
26
+ TCAGGTCTTTCAGAAGTTCTCCATATGTTCTCTTTGTCGTAGGCTCAACAAGGTCTTCAC
27
+ GGAGAAACCGGAAAATCAACTTTATATCACTTCTGACCTTTCCACTGAGACTTTTTAAGA
28
+ ATGCAAATTCTGGTGAGGAGACGCGAGTGACAGGAAATTCTTTTGGTAGTCCCAATATGT
29
+ AATCTTCGCCACTTAATCCGTGCTCCAGAAAAAACGAGTTGTACTAAAGAAAATATGAAC
30
+ TTCTATTAGATAGAGAACATGGTACAGTACAAAGAGATCCTTGGTGAATCTTTGAATGTT
31
+ AGTGAAACCACATCTACCTCTATGTTGACCCAAATTACATGAAGCTCGCCTTTTTGAGCC
32
+ AAGACGCTTTGAACCACATTGTCAAGCGACAACAGTTCCTGTGTTCTGTCAAATGTATTT
33
+ GAGCGAGACAAAACATTCTGAACCACTGAGGGATAATCAAACCACGTGACAAGTCAATAC
34
+ TTTGCAACAGGAAAAAAGAAGAAGAATTAAAAACAGAACCTAAATGGAAAATCAGAAATG
35
+ ATTTCAAAGGATCAACCATTTGGAAGAAAAAAACTTTCAAGGATCCATTTGAACCTTATT
36
+ CACTCCAAATATGGAAAAATATATGTGTTTTTACCACGTTTGCAATTGCTAACTTCATCA
37
+ TGGAGCATGGATAAAGTACTTTGATTTTACGGTTATGATTTTCAAGATAACCAAGATTAT
38
+ TTTTTCTGCTTACATAGGACACTATGTAGCTCCTCTGATTTGAAATCCAGAGAAAACCAT
39
+ CCTTGAACATCTCGTCCATTCACTTGATAAGTCGAGCCCCTGTCAGCGAACTTCTCAGCA
40
+ ATATTTGACGAATTGTCAAGTCTCAAGCTGCCATGGCAGAAGCCCACACCGTCGCTAGAG
41
+ AATTGCAGGTCGCAGAACAGAACCACCTCACACAAGCTACTTCCCATTGCCATGCTGTAT
42
+ GCAAGCTGGCTTGACTCAGGCACTACCCCAGACAAACCCCCACGAGCAATGACTAGAGGA
43
+ CGACGACCTGTAGGACAGTACTAGAAAGATTAGCAGCACGGCAGACAAACCATAATGTAG
44
+ GCTGAAAGAACATACTTTCTAGCTCACATTCTTCTATACAAAATACGCGCTTTTCCTTTG
45
+ TCGCATGCTAAACCaaaaaaaaaTCCCAATTCATAACTCAGCAATGGGCTAGATGCTTAC
46
+ CACTCAGAGTCAGCCATCCCGGTACTGGAGGATCCGAAGCAGCCTTGGTTCCATGAAGAA
47
+ GTAAGAGGATCAAGAAAACGAGAGGATATGTTGCTCCCATTCGGGTTAGAGACGAGCACC
48
+ CTGCTGCATCACACAAATTTCACAACAGATGCAACATACGTAAGTACCATGCGTTCACGA
49
+ GAGATGCAAGATAGACAAATCTGTGTCTCTTCACGAATCAATTCAGACAATAATTGCAGA
50
+ GGCGCAAGCAAAATAGTTGCAGAATTAAGGGAAAGGATGACAGCATCTCACCAGACCTTG
51
+ TGGGGAAATTCAGGCTTCAAATGTGCTAGCGCGACGGAGTGGGTTTGGGGAAAGGGATCT
52
+ AGCACCCACAAAACTAGAGGAACTAAATTGTGGATAGGCGGCAGGCAAGGCATGCATGCA
53
+ AGGTCTCTAAATCAGACTGTGGAGAGGAGGCAAAGGAGGAGGCAGAAGACGAGGCGAAAG
54
+ GCTTCTAAAAATAGGGGCCTCCCGGGCGATGGACAGGGTTTTGACATGGCATGGATGATC
55
+ GTCTCTCACTCACTTGTATGGCGAGTCTCTGCTCCACCTCAACAGCCAGAAAAACCTCCC
56
+ CTTTTATATAAAAATGTAAAAGGGAAAGAAACAGAGAGATGGTTTTCTTTTCGTGCCATT
57
+ GCTGGAGCCAGAGCAACTCCATCCAAGGACCTTAGACTAGATATCCTGGACGTGTTCGGT
58
+ GCCTTCTCGCCGCTCCCTTGCTGAACCTACATGCATATGTTCAGAATTCAGATAGATTAA
59
+ ATGGGACGGGACACAATGTATGTAAGATGAAAATTACACGATGCAGAGGTTGGTTTAAGA
60
+ AATCTAGCAAAATTTACAGAAATAACTGGTCATGAGAACATAAAAGGCCAGCATTGGATC
61
+ TGAGGCTTTGACCTCTGTATGCTTTCTCCCATTTCCCCAATTAGAGACGGCCGCGAAATC
62
+ TCACCACGAACGGAACGGACTACGGAGGCTGTGCCCGGGAGACGGGTCGAGCACGACGGA
63
+ GGGGAGGAATAACGACGAGGACATAGGGTTTTCTCCGACGGGAGTAATGGATGGACGGGG
64
+ AGGCGGCGGCGCTACGGAGCCCTGCATGCCCTAGGCGCCGAGAGGAGGAGCGCGGGGAGA
65
+ CGGCGGCCCTCGCCATCCGCCGCGTGCCGGTGGGAGCGGCGGGGATGGGTGGTGGAAGTG
66
+ GCGGGAGTTAGAGGGAGAAGACGGCACGGCGACATTGGATTCCGGCCGCCGGCAGACGGG
67
+ ACGAAGGCGAGGTTCTTCTCATCCCATCGTGTAGATAAGGTTAGCTTAGTCCCCATCGGC
68
+ GTTAATTGGAAGGGATCGACGTAATCCGCCACCTCCTCTTCACATTGGTGTTGCTTCGGG
69
+ GCGGCGCTGGGATGGGAGTGGCATGCCCTCCCGTCAATCTGCGGATCCGGTGGTTTGTGT
70
+ GCTCCTGGGATGGTCGACGTCTTGGTGGTGACACCGTGCTCCAGTTCTAGGCCCTCTCAG
71
+ GCGGCTTGGTGGTTGCTCTGGTCTACAATTCGTCGATGGCAGCAGCGGATCGGGCGCACC
72
+ CGGTGAAGCTCGATTTTGCTCCTCATGAATCCCACGAGTTTGTGTTCTCTGACTTGTTTG
73
+ GATATTCGTGCGATATCGACTTCCACAACTTCAGCTCATGCTCGGTCACGCTGGTCCTTC
74
+ GAGACCGTAGCACGGAGACTTCCAATCCGCGTTTACAAACAAAGTTTGGCCAGCCGAGGC
75
+ TCGGGAGCAGCAATGGTGGCAGCGACGGTGCACTTGTGGCTCGTGATGGAACTTCAAGCT
76
+ GATGTGCGTCTCAGGGACATCCTTGTAATTTTTTTCTTTGTGTGAGGTGCTTTGTGTTGA
77
+ TGTTTATCCTCTATAACAAATCTGATGGCTTATTTTTGaaaaaaaaGGAGGAAGCATTTT
78
+ GTTTGCAAGTACGGGCGGATAACGAGCATGTTTGGCTCCTTAAGGTTTAGAGGCTCGCTA
79
+ TCCTAACAAGATAAAATTTAAGCTCAGTTTGGATCGTTACATCCGCaaaaaaaaaGAAGA
80
+ AGTTTAGATCGTTAAAAAGCTCATGAGTGCTTGCAAACGCTAGTTACCGCTTCTCATACG
81
+ TCCAACACTATAGTGTCATTGTGCCATGAGTGTGTTTCATGATGACAACTCttttttcct
82
+ tttttGCAGGGTTTCATAATGACAACTCGCCTAATCAACTTTGCCACTATTCAACTCCGA
83
+ GACACACCAACAGAAGATTTGTGTTCTGTGATGCGGGCAAAGCCAATTGTGATTAAAAGG
84
+ TCTACACGCGGGCTAGTGAAAtactcccttcgtcttaaaataagtgtctcgaatttagta
85
+ caattatattaaatccgacctagaaaaatatgttgagaaggggagagtaCAAATGAGTTT
86
+ AAGGAGCTACTCATGAGCTCGATTGATAGGAACTTTAGCTAAGCTAAGAACAAATACGGA
87
+ CTTGATAAGCAGAAACGAGACGACACATGTTTGTAAACATCTACGTGCAACAACTCGTTA
88
+ CTTATTTTCAAGGGGGGCCCCGGGCTTCGAGTTCCTTATCAAGCACATGCAAGCTGATGG
89
+ GATGAACGTAAATTTCAGCGACCGACAACAGTAAAAAGGAGCTCAAAACCAAACCAATAT
90
+ ACTATATCATCACATTTATCTACTGCACTGCACTTAGTGTATGCACCACTCTATTGATCT
91
+ TTGCAACAATATTGGATGCGTGTACTCTACTTCCTACCGTAATTAATCTCCAGAGGTAGG
92
+ TATGATCCCCTTTTCAGGTCTGGTGTGTTCTGCTTTGAATTTGCTGCAGCCTCTAGCTCT
93
+ GCTATACATATGGGCTCTTGAATTTTGTGGTAGATGAACTAGCTACTAGCCAGATGACCT
94
+ TGCTAGTACTGTAGATGTAGCAGCAGAGGTGATCAAGTACGATTTTCTTATTTGTCTCCT
95
+ GTTTCACCCGATCTGGGGACTGGGGTACTATCTTTAATGCTGTGCCTCACGTTTAGTGGT
96
+ AGCTTGAACAAAGGTATTTTTGCATGATTTGAAGTTCATCCCAAAAGGCCTCCTCAAAAC
97
+ CCCTTCTTGTATTGGGACATTCAGACTTAATTTCAAGACAATATGAATGCATATTTTCTC
98
+ AGTGTTTCCTAGCTAGGATTGAGGGATATTTGGTGAAAAGTCTCATTGGCCTGGCTCGGA
99
+ TAATGGGATTTCATCTTTAACCCTTTCTTTCATTGCACTCTGGAGATTTGCCCTCTCCAA
100
+ TCTAAGTAACATGTACTTGGGACCTGAACAGAGCCTCTTCCCCAAGAAAAGTCTAGAGTA
101
+ CCCTtgcctcgtctccgatggccttaggaccatggcggcgcggtggatctcggtccttgt
102
+ cggcaggagggatttgttttcagttgttttttcgcgttttgttggggtttgtgtcctgct
103
+ taggaagacgagacggcggcggctccctgaagatggaataaggttctccccgtctagccc
104
+ ccgtcccgatggtgtgtctagtatcatcggtgggcgtgtggagatgcgcctccggcggat
105
+ ctgtccttggtggatttgctcggatctgctcgtagttcgtctacgttcatgtgttttcag
106
+ gttggatccttctgatctacgctactcttcatcggcggcgattgttgtgctgctgcgctg
107
+ gtcctatggggtcttagcacgatgacttcccggctatctactgcaacaagttttgcccga
108
+ ctccgactagagaggggcgatgacggcggcgcgccttcggctcgcttcagtgcttgtagt
109
+ cgtcgctaggtgttctacgaatctggatgtaatttttattatttttggtattcgttgcac
110
+ tgccatgattgaagatgaatagattggaagttttttcgcaaaaaaaTAATAATCTAAGTG
111
+ ACCTATCTTCGCACCCATACCCCCTATAAACCTCTATTGGAGTCCCTTTTCGGCTTTGAT
112
+ ATAAGTGACGATGTGCTATGCTCGTTTGTGAGAGGCGAGTGTGGTCAGTTGCGACCC