BioDSL 1.0.0

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Files changed (197) hide show
  1. checksums.yaml +7 -0
  2. data/.gitignore +10 -0
  3. data/BioDSL.gemspec +64 -0
  4. data/LICENSE +339 -0
  5. data/README.md +205 -0
  6. data/Rakefile +94 -0
  7. data/examples/fastq_to_fasta.rb +8 -0
  8. data/lib/BioDSL/cary.rb +242 -0
  9. data/lib/BioDSL/command.rb +133 -0
  10. data/lib/BioDSL/commands/add_key.rb +110 -0
  11. data/lib/BioDSL/commands/align_seq_mothur.rb +194 -0
  12. data/lib/BioDSL/commands/analyze_residue_distribution.rb +222 -0
  13. data/lib/BioDSL/commands/assemble_pairs.rb +336 -0
  14. data/lib/BioDSL/commands/assemble_seq_idba.rb +230 -0
  15. data/lib/BioDSL/commands/assemble_seq_ray.rb +345 -0
  16. data/lib/BioDSL/commands/assemble_seq_spades.rb +252 -0
  17. data/lib/BioDSL/commands/classify_seq.rb +217 -0
  18. data/lib/BioDSL/commands/classify_seq_mothur.rb +226 -0
  19. data/lib/BioDSL/commands/clip_primer.rb +318 -0
  20. data/lib/BioDSL/commands/cluster_otus.rb +181 -0
  21. data/lib/BioDSL/commands/collapse_otus.rb +170 -0
  22. data/lib/BioDSL/commands/collect_otus.rb +150 -0
  23. data/lib/BioDSL/commands/complement_seq.rb +117 -0
  24. data/lib/BioDSL/commands/count.rb +135 -0
  25. data/lib/BioDSL/commands/count_values.rb +149 -0
  26. data/lib/BioDSL/commands/degap_seq.rb +253 -0
  27. data/lib/BioDSL/commands/dereplicate_seq.rb +168 -0
  28. data/lib/BioDSL/commands/dump.rb +157 -0
  29. data/lib/BioDSL/commands/filter_rrna.rb +239 -0
  30. data/lib/BioDSL/commands/genecall.rb +237 -0
  31. data/lib/BioDSL/commands/grab.rb +535 -0
  32. data/lib/BioDSL/commands/index_taxonomy.rb +226 -0
  33. data/lib/BioDSL/commands/mask_seq.rb +175 -0
  34. data/lib/BioDSL/commands/mean_scores.rb +168 -0
  35. data/lib/BioDSL/commands/merge_pair_seq.rb +175 -0
  36. data/lib/BioDSL/commands/merge_table.rb +225 -0
  37. data/lib/BioDSL/commands/merge_values.rb +113 -0
  38. data/lib/BioDSL/commands/plot_heatmap.rb +233 -0
  39. data/lib/BioDSL/commands/plot_histogram.rb +306 -0
  40. data/lib/BioDSL/commands/plot_matches.rb +282 -0
  41. data/lib/BioDSL/commands/plot_residue_distribution.rb +278 -0
  42. data/lib/BioDSL/commands/plot_scores.rb +285 -0
  43. data/lib/BioDSL/commands/random.rb +153 -0
  44. data/lib/BioDSL/commands/read_fasta.rb +222 -0
  45. data/lib/BioDSL/commands/read_fastq.rb +414 -0
  46. data/lib/BioDSL/commands/read_table.rb +329 -0
  47. data/lib/BioDSL/commands/reverse_seq.rb +113 -0
  48. data/lib/BioDSL/commands/slice_align.rb +400 -0
  49. data/lib/BioDSL/commands/slice_seq.rb +151 -0
  50. data/lib/BioDSL/commands/sort.rb +223 -0
  51. data/lib/BioDSL/commands/split_pair_seq.rb +220 -0
  52. data/lib/BioDSL/commands/split_values.rb +165 -0
  53. data/lib/BioDSL/commands/trim_primer.rb +314 -0
  54. data/lib/BioDSL/commands/trim_seq.rb +192 -0
  55. data/lib/BioDSL/commands/uchime_ref.rb +170 -0
  56. data/lib/BioDSL/commands/uclust.rb +286 -0
  57. data/lib/BioDSL/commands/unique_values.rb +145 -0
  58. data/lib/BioDSL/commands/usearch_global.rb +171 -0
  59. data/lib/BioDSL/commands/usearch_local.rb +171 -0
  60. data/lib/BioDSL/commands/write_fasta.rb +207 -0
  61. data/lib/BioDSL/commands/write_fastq.rb +191 -0
  62. data/lib/BioDSL/commands/write_table.rb +419 -0
  63. data/lib/BioDSL/commands/write_tree.rb +167 -0
  64. data/lib/BioDSL/commands.rb +31 -0
  65. data/lib/BioDSL/config.rb +55 -0
  66. data/lib/BioDSL/csv.rb +307 -0
  67. data/lib/BioDSL/debug.rb +42 -0
  68. data/lib/BioDSL/fasta.rb +133 -0
  69. data/lib/BioDSL/fastq.rb +77 -0
  70. data/lib/BioDSL/filesys.rb +137 -0
  71. data/lib/BioDSL/fork.rb +145 -0
  72. data/lib/BioDSL/hamming.rb +128 -0
  73. data/lib/BioDSL/helpers/aux_helper.rb +44 -0
  74. data/lib/BioDSL/helpers/email_helper.rb +66 -0
  75. data/lib/BioDSL/helpers/history_helper.rb +40 -0
  76. data/lib/BioDSL/helpers/log_helper.rb +55 -0
  77. data/lib/BioDSL/helpers/options_helper.rb +405 -0
  78. data/lib/BioDSL/helpers/status_helper.rb +132 -0
  79. data/lib/BioDSL/helpers.rb +35 -0
  80. data/lib/BioDSL/html_report.rb +200 -0
  81. data/lib/BioDSL/math.rb +55 -0
  82. data/lib/BioDSL/mummer.rb +216 -0
  83. data/lib/BioDSL/pipeline.rb +354 -0
  84. data/lib/BioDSL/seq/ambiguity.rb +66 -0
  85. data/lib/BioDSL/seq/assemble.rb +240 -0
  86. data/lib/BioDSL/seq/backtrack.rb +252 -0
  87. data/lib/BioDSL/seq/digest.rb +99 -0
  88. data/lib/BioDSL/seq/dynamic.rb +263 -0
  89. data/lib/BioDSL/seq/homopolymer.rb +59 -0
  90. data/lib/BioDSL/seq/kmer.rb +293 -0
  91. data/lib/BioDSL/seq/levenshtein.rb +113 -0
  92. data/lib/BioDSL/seq/translate.rb +109 -0
  93. data/lib/BioDSL/seq/trim.rb +188 -0
  94. data/lib/BioDSL/seq.rb +742 -0
  95. data/lib/BioDSL/serializer.rb +98 -0
  96. data/lib/BioDSL/stream.rb +113 -0
  97. data/lib/BioDSL/taxonomy.rb +691 -0
  98. data/lib/BioDSL/test.rb +42 -0
  99. data/lib/BioDSL/tmp_dir.rb +68 -0
  100. data/lib/BioDSL/usearch.rb +301 -0
  101. data/lib/BioDSL/verbose.rb +42 -0
  102. data/lib/BioDSL/version.rb +31 -0
  103. data/lib/BioDSL.rb +81 -0
  104. data/test/BioDSL/commands/test_add_key.rb +105 -0
  105. data/test/BioDSL/commands/test_align_seq_mothur.rb +99 -0
  106. data/test/BioDSL/commands/test_analyze_residue_distribution.rb +134 -0
  107. data/test/BioDSL/commands/test_assemble_pairs.rb +459 -0
  108. data/test/BioDSL/commands/test_assemble_seq_idba.rb +50 -0
  109. data/test/BioDSL/commands/test_assemble_seq_ray.rb +51 -0
  110. data/test/BioDSL/commands/test_assemble_seq_spades.rb +50 -0
  111. data/test/BioDSL/commands/test_classify_seq.rb +50 -0
  112. data/test/BioDSL/commands/test_classify_seq_mothur.rb +59 -0
  113. data/test/BioDSL/commands/test_clip_primer.rb +377 -0
  114. data/test/BioDSL/commands/test_cluster_otus.rb +128 -0
  115. data/test/BioDSL/commands/test_collapse_otus.rb +81 -0
  116. data/test/BioDSL/commands/test_collect_otus.rb +82 -0
  117. data/test/BioDSL/commands/test_complement_seq.rb +78 -0
  118. data/test/BioDSL/commands/test_count.rb +103 -0
  119. data/test/BioDSL/commands/test_count_values.rb +85 -0
  120. data/test/BioDSL/commands/test_degap_seq.rb +96 -0
  121. data/test/BioDSL/commands/test_dereplicate_seq.rb +92 -0
  122. data/test/BioDSL/commands/test_dump.rb +109 -0
  123. data/test/BioDSL/commands/test_filter_rrna.rb +128 -0
  124. data/test/BioDSL/commands/test_genecall.rb +50 -0
  125. data/test/BioDSL/commands/test_grab.rb +398 -0
  126. data/test/BioDSL/commands/test_index_taxonomy.rb +62 -0
  127. data/test/BioDSL/commands/test_mask_seq.rb +98 -0
  128. data/test/BioDSL/commands/test_mean_scores.rb +111 -0
  129. data/test/BioDSL/commands/test_merge_pair_seq.rb +115 -0
  130. data/test/BioDSL/commands/test_merge_table.rb +131 -0
  131. data/test/BioDSL/commands/test_merge_values.rb +83 -0
  132. data/test/BioDSL/commands/test_plot_heatmap.rb +185 -0
  133. data/test/BioDSL/commands/test_plot_histogram.rb +194 -0
  134. data/test/BioDSL/commands/test_plot_matches.rb +157 -0
  135. data/test/BioDSL/commands/test_plot_residue_distribution.rb +309 -0
  136. data/test/BioDSL/commands/test_plot_scores.rb +308 -0
  137. data/test/BioDSL/commands/test_random.rb +88 -0
  138. data/test/BioDSL/commands/test_read_fasta.rb +229 -0
  139. data/test/BioDSL/commands/test_read_fastq.rb +552 -0
  140. data/test/BioDSL/commands/test_read_table.rb +327 -0
  141. data/test/BioDSL/commands/test_reverse_seq.rb +79 -0
  142. data/test/BioDSL/commands/test_slice_align.rb +218 -0
  143. data/test/BioDSL/commands/test_slice_seq.rb +131 -0
  144. data/test/BioDSL/commands/test_sort.rb +128 -0
  145. data/test/BioDSL/commands/test_split_pair_seq.rb +164 -0
  146. data/test/BioDSL/commands/test_split_values.rb +95 -0
  147. data/test/BioDSL/commands/test_trim_primer.rb +329 -0
  148. data/test/BioDSL/commands/test_trim_seq.rb +150 -0
  149. data/test/BioDSL/commands/test_uchime_ref.rb +113 -0
  150. data/test/BioDSL/commands/test_uclust.rb +139 -0
  151. data/test/BioDSL/commands/test_unique_values.rb +98 -0
  152. data/test/BioDSL/commands/test_usearch_global.rb +123 -0
  153. data/test/BioDSL/commands/test_usearch_local.rb +125 -0
  154. data/test/BioDSL/commands/test_write_fasta.rb +159 -0
  155. data/test/BioDSL/commands/test_write_fastq.rb +166 -0
  156. data/test/BioDSL/commands/test_write_table.rb +411 -0
  157. data/test/BioDSL/commands/test_write_tree.rb +122 -0
  158. data/test/BioDSL/helpers/test_options_helper.rb +272 -0
  159. data/test/BioDSL/seq/test_assemble.rb +98 -0
  160. data/test/BioDSL/seq/test_backtrack.rb +176 -0
  161. data/test/BioDSL/seq/test_digest.rb +71 -0
  162. data/test/BioDSL/seq/test_dynamic.rb +133 -0
  163. data/test/BioDSL/seq/test_homopolymer.rb +58 -0
  164. data/test/BioDSL/seq/test_kmer.rb +134 -0
  165. data/test/BioDSL/seq/test_translate.rb +75 -0
  166. data/test/BioDSL/seq/test_trim.rb +101 -0
  167. data/test/BioDSL/test_cary.rb +176 -0
  168. data/test/BioDSL/test_command.rb +45 -0
  169. data/test/BioDSL/test_csv.rb +514 -0
  170. data/test/BioDSL/test_debug.rb +42 -0
  171. data/test/BioDSL/test_fasta.rb +154 -0
  172. data/test/BioDSL/test_fastq.rb +46 -0
  173. data/test/BioDSL/test_filesys.rb +145 -0
  174. data/test/BioDSL/test_fork.rb +85 -0
  175. data/test/BioDSL/test_math.rb +41 -0
  176. data/test/BioDSL/test_mummer.rb +79 -0
  177. data/test/BioDSL/test_pipeline.rb +187 -0
  178. data/test/BioDSL/test_seq.rb +790 -0
  179. data/test/BioDSL/test_serializer.rb +72 -0
  180. data/test/BioDSL/test_stream.rb +55 -0
  181. data/test/BioDSL/test_taxonomy.rb +336 -0
  182. data/test/BioDSL/test_test.rb +42 -0
  183. data/test/BioDSL/test_tmp_dir.rb +58 -0
  184. data/test/BioDSL/test_usearch.rb +33 -0
  185. data/test/BioDSL/test_verbose.rb +42 -0
  186. data/test/helper.rb +82 -0
  187. data/www/command.html.haml +14 -0
  188. data/www/css.html.haml +55 -0
  189. data/www/input_files.html.haml +3 -0
  190. data/www/layout.html.haml +12 -0
  191. data/www/output_files.html.haml +3 -0
  192. data/www/overview.html.haml +15 -0
  193. data/www/pipeline.html.haml +4 -0
  194. data/www/png.html.haml +2 -0
  195. data/www/status.html.haml +9 -0
  196. data/www/time.html.haml +11 -0
  197. metadata +503 -0
data/test/helper.rb ADDED
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+ # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< #
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+ # #
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+ # Copyright (C) 2007-2015 Martin Asser Hansen (mail@maasha.dk). #
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+ # #
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+ # This program is free software; you can redistribute it and/or #
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+ # modify it under the terms of the GNU General Public License #
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+ # as published by the Free Software Foundation; either version 2 #
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+ # of the License, or (at your option) any later version. #
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+ # #
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+ # This program is distributed in the hope that it will be useful, #
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+ # but WITHOUT ANY WARRANTY; without even the implied warranty of #
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+ # MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the #
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+ # GNU General Public License for more details. #
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+ # #
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+ # You should have received a copy of the GNU General Public License #
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+ # along with this program; if not, write to the Free Software #
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+ # Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA. #
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+ # #
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+ # http://www.gnu.org/copyleft/gpl.html #
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+ # #
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+ # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< #
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+ # #
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+ # This software is part of the BioDSL framework (www.BioDSL.org). #
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+ # #
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+ # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< #
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+
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+ require 'simplecov'
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+
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+ if ENV['SIMPLECOV']
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+ SimpleCov.start do
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+ add_filter "/test/"
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+ end
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+
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+ SimpleCov.command_name 'test:units'
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+ end
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+
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+ require 'pp'
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+ require 'tempfile'
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+ require 'fileutils'
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+ require 'BioDSL'
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+ require 'test/unit'
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+ require 'mocha/test_unit'
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+
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+ ENV['BP_TEST'] = "true"
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+
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+ module Kernel
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+ def capture_stdout
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+ out = StringIO.new
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+ $stdout = out
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+ yield
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+ return out.string
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+ ensure
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+ $stdout = STDOUT
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+ end
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+
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+ def capture_stderr
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+ out = StringIO.new
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+ $stderr = out
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+ yield
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+ return out.string
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+ ensure
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+ $stderr = STDERR
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+ end
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+ end
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+
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+ class Test::Unit::TestCase
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+ # Ruby 2.2 have omit, < 2.2 have skip
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+ alias :omit :skip if ! self.instance_methods.include? :omit
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+
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+ def self.test(desc, &impl)
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+ define_method("test #{desc}", &impl)
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+ end
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+
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+ def collect_result
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+ @input2.each_with_object('') { |e, a| a << "#{e}#{$/}" }
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+ end
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+
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+ def collect_sorted_result
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+ @input2.sort_by { |a| a.to_s }.
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+ each_with_object('') { |e, a| a << "#{e}#{$/}" }
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+ end
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+ end
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+ .section
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+ %a{:name => "#{command.name}#{index}"}
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+ %h2
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+ Command: #{command.name}
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+ %a{:href => help_url(command.name), :title => "Click for help on #{command.name}", :target => "_blank"} ?
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+ %p.command #{command.to_s}
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+ = render_status command
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+ = render_time command.status
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+ - if input? command.options
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+ = render_input_files command.options
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+ - if output? command.options
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+ = render_output_files command.options
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+ - if png? command.options
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+ = render_png command.options
data/www/css.html.haml ADDED
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+ :css
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+ .center {
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+ text-align: center;
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+ }
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+
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+ .right {
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+ text-align: right;
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+ }
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+
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+ .left {
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+ text-align: left;
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+ }
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+
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+ .bold {
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+ font-weight: bold;
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+ }
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+
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+ img {
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+ display: block;
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+ margin-left: auto;
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+ margin-right: auto;
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+ }
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+
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+ table {
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+ border-collapse: collapse;
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+ }
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+
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+ th {
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+ padding-left: 10px;
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+ padding-right: 10px;
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+ }
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+
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+ td {
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+ padding-left: 10px;
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+ padding-right: 10px;
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+ }
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+
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+ body {
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+ font-family: Arial, Helvetica, Verdana, sans-serif;
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+ min-width: 1000px;
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+ margin-left: 10%;
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+ margin-right: 10%;
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+ }
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+
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+ .section {
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+ padding-bottom: 1em;
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+ border-bottom: 2px solid #ccc;
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+ }
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+
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+ .command {
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+ font-family: Courier, monospace, sans-serif;
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+ border: 1px solid black;
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+ padding: 1em;
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+ white-space: pre;
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+ }
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+ %h3 Input
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+ - files.each do |file|
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+ %p #{file}
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+ !!!
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+ %head
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+ %title BioDSL report
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+ = render_css
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+ %body
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+ %h1.center BioDSL report
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+ %p.center.bold Version #{BioDSL::VERSION}
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+ %p.center.bold Generated #{Time.now}
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+ = render_pipeline pipeline
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+ = render_overview commands
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+ - commands.each_with_index do |command, i|
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+ = render_command command, i
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+ %h3 Output
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+ %p
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+ %a{:href => options[:output].split('/').last} #{options[:output].split('/').last}
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+ .section
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+ %h2 Overview
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+ %table
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+ %tr.left
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+ %th.bold Command
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+ %th.bold Records in
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+ %th.bold Records out
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+ %th.bold Time elapsed
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+ - commands.each_with_index do |command, i|
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+ %tr
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+ %td
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+ %a{:href => "##{command.name}#{i}"} #{command.name}
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+ %td.right #{command.status[:records_in].commify}
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+ %td.right #{command.status[:records_out].commify}
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+ %td.right #{command.status[:time_elapsed]}
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+ .section
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+ %h2 Pipeline
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+ .command
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+ = pipeline
data/www/png.html.haml ADDED
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+ %p
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+ %img{:src => png_data}/
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+ %h3 Status: #{exit_status}
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+ %table
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+ - statsus.each do |key, val|
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+ %tr
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+ %td.bold #{key}
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+ - if val.is_a? Numeric
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+ %td.right #{val.commify}
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+ - else
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+ %td.right #{val}
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+ %h3 Time
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+ %table
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+ %tr
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+ %td.bold Time start
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+ %td.right #{status[:time_start]}
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+ %tr
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+ %td.bold Time stop
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+ %td.right #{status[:time_stop]}
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+ %tr
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+ %td.bold Time elapsed:
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+ %td.right #{status[:time_elapsed]}