BioDSL 1.0.0

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Files changed (197) hide show
  1. checksums.yaml +7 -0
  2. data/.gitignore +10 -0
  3. data/BioDSL.gemspec +64 -0
  4. data/LICENSE +339 -0
  5. data/README.md +205 -0
  6. data/Rakefile +94 -0
  7. data/examples/fastq_to_fasta.rb +8 -0
  8. data/lib/BioDSL/cary.rb +242 -0
  9. data/lib/BioDSL/command.rb +133 -0
  10. data/lib/BioDSL/commands/add_key.rb +110 -0
  11. data/lib/BioDSL/commands/align_seq_mothur.rb +194 -0
  12. data/lib/BioDSL/commands/analyze_residue_distribution.rb +222 -0
  13. data/lib/BioDSL/commands/assemble_pairs.rb +336 -0
  14. data/lib/BioDSL/commands/assemble_seq_idba.rb +230 -0
  15. data/lib/BioDSL/commands/assemble_seq_ray.rb +345 -0
  16. data/lib/BioDSL/commands/assemble_seq_spades.rb +252 -0
  17. data/lib/BioDSL/commands/classify_seq.rb +217 -0
  18. data/lib/BioDSL/commands/classify_seq_mothur.rb +226 -0
  19. data/lib/BioDSL/commands/clip_primer.rb +318 -0
  20. data/lib/BioDSL/commands/cluster_otus.rb +181 -0
  21. data/lib/BioDSL/commands/collapse_otus.rb +170 -0
  22. data/lib/BioDSL/commands/collect_otus.rb +150 -0
  23. data/lib/BioDSL/commands/complement_seq.rb +117 -0
  24. data/lib/BioDSL/commands/count.rb +135 -0
  25. data/lib/BioDSL/commands/count_values.rb +149 -0
  26. data/lib/BioDSL/commands/degap_seq.rb +253 -0
  27. data/lib/BioDSL/commands/dereplicate_seq.rb +168 -0
  28. data/lib/BioDSL/commands/dump.rb +157 -0
  29. data/lib/BioDSL/commands/filter_rrna.rb +239 -0
  30. data/lib/BioDSL/commands/genecall.rb +237 -0
  31. data/lib/BioDSL/commands/grab.rb +535 -0
  32. data/lib/BioDSL/commands/index_taxonomy.rb +226 -0
  33. data/lib/BioDSL/commands/mask_seq.rb +175 -0
  34. data/lib/BioDSL/commands/mean_scores.rb +168 -0
  35. data/lib/BioDSL/commands/merge_pair_seq.rb +175 -0
  36. data/lib/BioDSL/commands/merge_table.rb +225 -0
  37. data/lib/BioDSL/commands/merge_values.rb +113 -0
  38. data/lib/BioDSL/commands/plot_heatmap.rb +233 -0
  39. data/lib/BioDSL/commands/plot_histogram.rb +306 -0
  40. data/lib/BioDSL/commands/plot_matches.rb +282 -0
  41. data/lib/BioDSL/commands/plot_residue_distribution.rb +278 -0
  42. data/lib/BioDSL/commands/plot_scores.rb +285 -0
  43. data/lib/BioDSL/commands/random.rb +153 -0
  44. data/lib/BioDSL/commands/read_fasta.rb +222 -0
  45. data/lib/BioDSL/commands/read_fastq.rb +414 -0
  46. data/lib/BioDSL/commands/read_table.rb +329 -0
  47. data/lib/BioDSL/commands/reverse_seq.rb +113 -0
  48. data/lib/BioDSL/commands/slice_align.rb +400 -0
  49. data/lib/BioDSL/commands/slice_seq.rb +151 -0
  50. data/lib/BioDSL/commands/sort.rb +223 -0
  51. data/lib/BioDSL/commands/split_pair_seq.rb +220 -0
  52. data/lib/BioDSL/commands/split_values.rb +165 -0
  53. data/lib/BioDSL/commands/trim_primer.rb +314 -0
  54. data/lib/BioDSL/commands/trim_seq.rb +192 -0
  55. data/lib/BioDSL/commands/uchime_ref.rb +170 -0
  56. data/lib/BioDSL/commands/uclust.rb +286 -0
  57. data/lib/BioDSL/commands/unique_values.rb +145 -0
  58. data/lib/BioDSL/commands/usearch_global.rb +171 -0
  59. data/lib/BioDSL/commands/usearch_local.rb +171 -0
  60. data/lib/BioDSL/commands/write_fasta.rb +207 -0
  61. data/lib/BioDSL/commands/write_fastq.rb +191 -0
  62. data/lib/BioDSL/commands/write_table.rb +419 -0
  63. data/lib/BioDSL/commands/write_tree.rb +167 -0
  64. data/lib/BioDSL/commands.rb +31 -0
  65. data/lib/BioDSL/config.rb +55 -0
  66. data/lib/BioDSL/csv.rb +307 -0
  67. data/lib/BioDSL/debug.rb +42 -0
  68. data/lib/BioDSL/fasta.rb +133 -0
  69. data/lib/BioDSL/fastq.rb +77 -0
  70. data/lib/BioDSL/filesys.rb +137 -0
  71. data/lib/BioDSL/fork.rb +145 -0
  72. data/lib/BioDSL/hamming.rb +128 -0
  73. data/lib/BioDSL/helpers/aux_helper.rb +44 -0
  74. data/lib/BioDSL/helpers/email_helper.rb +66 -0
  75. data/lib/BioDSL/helpers/history_helper.rb +40 -0
  76. data/lib/BioDSL/helpers/log_helper.rb +55 -0
  77. data/lib/BioDSL/helpers/options_helper.rb +405 -0
  78. data/lib/BioDSL/helpers/status_helper.rb +132 -0
  79. data/lib/BioDSL/helpers.rb +35 -0
  80. data/lib/BioDSL/html_report.rb +200 -0
  81. data/lib/BioDSL/math.rb +55 -0
  82. data/lib/BioDSL/mummer.rb +216 -0
  83. data/lib/BioDSL/pipeline.rb +354 -0
  84. data/lib/BioDSL/seq/ambiguity.rb +66 -0
  85. data/lib/BioDSL/seq/assemble.rb +240 -0
  86. data/lib/BioDSL/seq/backtrack.rb +252 -0
  87. data/lib/BioDSL/seq/digest.rb +99 -0
  88. data/lib/BioDSL/seq/dynamic.rb +263 -0
  89. data/lib/BioDSL/seq/homopolymer.rb +59 -0
  90. data/lib/BioDSL/seq/kmer.rb +293 -0
  91. data/lib/BioDSL/seq/levenshtein.rb +113 -0
  92. data/lib/BioDSL/seq/translate.rb +109 -0
  93. data/lib/BioDSL/seq/trim.rb +188 -0
  94. data/lib/BioDSL/seq.rb +742 -0
  95. data/lib/BioDSL/serializer.rb +98 -0
  96. data/lib/BioDSL/stream.rb +113 -0
  97. data/lib/BioDSL/taxonomy.rb +691 -0
  98. data/lib/BioDSL/test.rb +42 -0
  99. data/lib/BioDSL/tmp_dir.rb +68 -0
  100. data/lib/BioDSL/usearch.rb +301 -0
  101. data/lib/BioDSL/verbose.rb +42 -0
  102. data/lib/BioDSL/version.rb +31 -0
  103. data/lib/BioDSL.rb +81 -0
  104. data/test/BioDSL/commands/test_add_key.rb +105 -0
  105. data/test/BioDSL/commands/test_align_seq_mothur.rb +99 -0
  106. data/test/BioDSL/commands/test_analyze_residue_distribution.rb +134 -0
  107. data/test/BioDSL/commands/test_assemble_pairs.rb +459 -0
  108. data/test/BioDSL/commands/test_assemble_seq_idba.rb +50 -0
  109. data/test/BioDSL/commands/test_assemble_seq_ray.rb +51 -0
  110. data/test/BioDSL/commands/test_assemble_seq_spades.rb +50 -0
  111. data/test/BioDSL/commands/test_classify_seq.rb +50 -0
  112. data/test/BioDSL/commands/test_classify_seq_mothur.rb +59 -0
  113. data/test/BioDSL/commands/test_clip_primer.rb +377 -0
  114. data/test/BioDSL/commands/test_cluster_otus.rb +128 -0
  115. data/test/BioDSL/commands/test_collapse_otus.rb +81 -0
  116. data/test/BioDSL/commands/test_collect_otus.rb +82 -0
  117. data/test/BioDSL/commands/test_complement_seq.rb +78 -0
  118. data/test/BioDSL/commands/test_count.rb +103 -0
  119. data/test/BioDSL/commands/test_count_values.rb +85 -0
  120. data/test/BioDSL/commands/test_degap_seq.rb +96 -0
  121. data/test/BioDSL/commands/test_dereplicate_seq.rb +92 -0
  122. data/test/BioDSL/commands/test_dump.rb +109 -0
  123. data/test/BioDSL/commands/test_filter_rrna.rb +128 -0
  124. data/test/BioDSL/commands/test_genecall.rb +50 -0
  125. data/test/BioDSL/commands/test_grab.rb +398 -0
  126. data/test/BioDSL/commands/test_index_taxonomy.rb +62 -0
  127. data/test/BioDSL/commands/test_mask_seq.rb +98 -0
  128. data/test/BioDSL/commands/test_mean_scores.rb +111 -0
  129. data/test/BioDSL/commands/test_merge_pair_seq.rb +115 -0
  130. data/test/BioDSL/commands/test_merge_table.rb +131 -0
  131. data/test/BioDSL/commands/test_merge_values.rb +83 -0
  132. data/test/BioDSL/commands/test_plot_heatmap.rb +185 -0
  133. data/test/BioDSL/commands/test_plot_histogram.rb +194 -0
  134. data/test/BioDSL/commands/test_plot_matches.rb +157 -0
  135. data/test/BioDSL/commands/test_plot_residue_distribution.rb +309 -0
  136. data/test/BioDSL/commands/test_plot_scores.rb +308 -0
  137. data/test/BioDSL/commands/test_random.rb +88 -0
  138. data/test/BioDSL/commands/test_read_fasta.rb +229 -0
  139. data/test/BioDSL/commands/test_read_fastq.rb +552 -0
  140. data/test/BioDSL/commands/test_read_table.rb +327 -0
  141. data/test/BioDSL/commands/test_reverse_seq.rb +79 -0
  142. data/test/BioDSL/commands/test_slice_align.rb +218 -0
  143. data/test/BioDSL/commands/test_slice_seq.rb +131 -0
  144. data/test/BioDSL/commands/test_sort.rb +128 -0
  145. data/test/BioDSL/commands/test_split_pair_seq.rb +164 -0
  146. data/test/BioDSL/commands/test_split_values.rb +95 -0
  147. data/test/BioDSL/commands/test_trim_primer.rb +329 -0
  148. data/test/BioDSL/commands/test_trim_seq.rb +150 -0
  149. data/test/BioDSL/commands/test_uchime_ref.rb +113 -0
  150. data/test/BioDSL/commands/test_uclust.rb +139 -0
  151. data/test/BioDSL/commands/test_unique_values.rb +98 -0
  152. data/test/BioDSL/commands/test_usearch_global.rb +123 -0
  153. data/test/BioDSL/commands/test_usearch_local.rb +125 -0
  154. data/test/BioDSL/commands/test_write_fasta.rb +159 -0
  155. data/test/BioDSL/commands/test_write_fastq.rb +166 -0
  156. data/test/BioDSL/commands/test_write_table.rb +411 -0
  157. data/test/BioDSL/commands/test_write_tree.rb +122 -0
  158. data/test/BioDSL/helpers/test_options_helper.rb +272 -0
  159. data/test/BioDSL/seq/test_assemble.rb +98 -0
  160. data/test/BioDSL/seq/test_backtrack.rb +176 -0
  161. data/test/BioDSL/seq/test_digest.rb +71 -0
  162. data/test/BioDSL/seq/test_dynamic.rb +133 -0
  163. data/test/BioDSL/seq/test_homopolymer.rb +58 -0
  164. data/test/BioDSL/seq/test_kmer.rb +134 -0
  165. data/test/BioDSL/seq/test_translate.rb +75 -0
  166. data/test/BioDSL/seq/test_trim.rb +101 -0
  167. data/test/BioDSL/test_cary.rb +176 -0
  168. data/test/BioDSL/test_command.rb +45 -0
  169. data/test/BioDSL/test_csv.rb +514 -0
  170. data/test/BioDSL/test_debug.rb +42 -0
  171. data/test/BioDSL/test_fasta.rb +154 -0
  172. data/test/BioDSL/test_fastq.rb +46 -0
  173. data/test/BioDSL/test_filesys.rb +145 -0
  174. data/test/BioDSL/test_fork.rb +85 -0
  175. data/test/BioDSL/test_math.rb +41 -0
  176. data/test/BioDSL/test_mummer.rb +79 -0
  177. data/test/BioDSL/test_pipeline.rb +187 -0
  178. data/test/BioDSL/test_seq.rb +790 -0
  179. data/test/BioDSL/test_serializer.rb +72 -0
  180. data/test/BioDSL/test_stream.rb +55 -0
  181. data/test/BioDSL/test_taxonomy.rb +336 -0
  182. data/test/BioDSL/test_test.rb +42 -0
  183. data/test/BioDSL/test_tmp_dir.rb +58 -0
  184. data/test/BioDSL/test_usearch.rb +33 -0
  185. data/test/BioDSL/test_verbose.rb +42 -0
  186. data/test/helper.rb +82 -0
  187. data/www/command.html.haml +14 -0
  188. data/www/css.html.haml +55 -0
  189. data/www/input_files.html.haml +3 -0
  190. data/www/layout.html.haml +12 -0
  191. data/www/output_files.html.haml +3 -0
  192. data/www/overview.html.haml +15 -0
  193. data/www/pipeline.html.haml +4 -0
  194. data/www/png.html.haml +2 -0
  195. data/www/status.html.haml +9 -0
  196. data/www/time.html.haml +11 -0
  197. metadata +503 -0
@@ -0,0 +1,229 @@
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+ #!/usr/bin/env ruby
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+ $LOAD_PATH.unshift File.join(File.dirname(__FILE__), '..', '..', '..')
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+
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+ # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< #
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+ # #
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+ # Copyright (C) 2007-2015 Martin Asser Hansen (mail@maasha.dk). #
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+ # #
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+ # This program is free software; you can redistribute it and/or #
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+ # modify it under the terms of the GNU General Public License #
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+ # as published by the Free Software Foundation; either version 2 #
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+ # of the License, or (at your option) any later version. #
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+ # #
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+ # This program is distributed in the hope that it will be useful, #
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+ # but WITHOUT ANY WARRANTY; without even the implied warranty of #
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+ # MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the #
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+ # GNU General Public License for more details. #
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+ # #
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+ # You should have received a copy of the GNU General Public License #
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+ # along with this program; if not, write to the Free Software #
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+ # Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, #
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+ # USA. #
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+ # #
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+ # http://www.gnu.org/copyleft/gpl.html #
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+ # #
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+ # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< #
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+ # #
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+ # This software is part of BioDSL (www.github.com/maasha/BioDSL). #
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+ # #
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+ # >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< #
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+
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+ require 'test/helper'
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+
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+ # Test class for the read_fasta command.
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+ # rubocop:disable ClassLength
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+ class TestReadFasta < Test::Unit::TestCase
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+ def setup
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+ @tmpdir = Dir.mktmpdir('BioDSL')
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+
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+ write_fasta_data1
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+ write_fasta_data2
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+
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+ @input, @output = BioDSL::Stream.pipe
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+ @input2, @output2 = BioDSL::Stream.pipe
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+
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+ write_stream_data
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+
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+ @p = BioDSL::Pipeline.new
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+ @err = BioDSL::OptionError
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+ end
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+
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+ def write_fasta_data1
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+ @file = File.join(@tmpdir, 'test.fna')
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+
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+ File.open(@file, 'w') do |ios|
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+ ios.puts <<-EOF.gsub(/^\s+\|/, '')
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+ |>test1
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+ |atgcagcac
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+ |>test2
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+ |acagcactgA
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+ EOF
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+ end
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+ end
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+
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+ def write_fasta_data2
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+ @file2 = File.join(@tmpdir, 'test2.fna')
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+
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+ File.open(@file2, 'w') do |ios|
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+ ios.puts <<-EOF.gsub(/^\s+\|/, '')
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+ |>test3
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+ |acGTAagcac
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+ |>test4
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+ |aCCAgcactgA
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+ EOF
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+ end
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+ end
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+
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+ def write_stream_data
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+ @output.write(SEQ_NAME: 'test1', SEQ: 'atgcagcac', SEQ_LEN: 9)
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+ @output.write(SEQ_NAME: 'test2', SEQ: 'acagcactgA', SEQ_LEN: 10)
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+ @output.close
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+ end
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+
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+ def teardown
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+ FileUtils.rm_r @tmpdir
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+ end
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+
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+ test 'BioDSL::Pipeline::ReadFasta with invalid options raises' do
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+ assert_raise(@err) { @p.read_fasta(foo: 'bar') }
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+ end
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+
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+ test 'BioDSL::Pipeline::ReadFasta without required options raises' do
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+ assert_raise(@err) { @p.read_fasta }
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+ end
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+
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+ test 'BioDSL::Pipeline::ReadFasta with bad first raises' do
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+ assert_raise(@err) { @p.read_fasta(input: @file, first: -1) }
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+ end
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+
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+ test 'BioDSL::Pipeline::ReadFasta with bad last raises' do
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+ assert_raise(@err) { @p.read_fasta(input: @file, last: -1) }
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+ end
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+
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+ test 'BioDSL::Pipeline::ReadFasta with exclusive unique options raises' do
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+ assert_raise(@err) { @p.read_fasta(input: @file, first: 1, last: 1) }
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+ end
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+
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+ test 'BioDSL::Pipeline::ReadFasta with non-existing input file raises' do
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+ assert_raise(@err) { @p.read_fasta(input: '___adsf') }
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+ end
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+
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+ test 'BioDSL::Pipeline::ReadFasta returns correctly' do
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+ @p.read_fasta(input: @file).run(output: @output2)
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+
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+ expected = <<-EXP.gsub(/^\s+\|/, '')
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+ |{:SEQ_NAME=>"test1", :SEQ=>"atgcagcac", :SEQ_LEN=>9}
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+ |{:SEQ_NAME=>"test2", :SEQ=>"acagcactgA", :SEQ_LEN=>10}
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+ EXP
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+
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+ assert_equal(expected, collect_result)
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+ end
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+
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+ test 'BioDSL::Pipeline::ReadFasta status returns correctly' do
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+ @p.read_fasta(input: @file).run(output: @output2)
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+
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+ assert_equal(0, @p.status.first[:records_in])
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+ assert_equal(2, @p.status.first[:records_out])
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+ assert_equal(0, @p.status.first[:sequences_in])
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+ assert_equal(2, @p.status.first[:sequences_out])
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+ assert_equal(0, @p.status.first[:residues_in])
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+ assert_equal(19, @p.status.first[:residues_out])
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+ end
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+
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+ test 'BioDSL::Pipeline::ReadFasta with gzipped data returns correctly' do
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+ `gzip #{@file}`
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+
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+ @p.read_fasta(input: "#{@file}.gz").run(output: @output2)
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+
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+ expected = <<-EXP.gsub(/^\s+\|/, '')
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+ |{:SEQ_NAME=>"test1", :SEQ=>"atgcagcac", :SEQ_LEN=>9}
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+ |{:SEQ_NAME=>"test2", :SEQ=>"acagcactgA", :SEQ_LEN=>10}
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+ EXP
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+
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+ assert_equal(expected, collect_result)
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+ end
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+
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+ test 'BioDSL::Pipeline::ReadFasta with bzip2\'ed data returns correctly' do
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+ `bzip2 #{@file}`
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+
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+ @p.read_fasta(input: "#{@file}.bz2").run(output: @output2)
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+
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+ expected = <<-EXP.gsub(/^\s+\|/, '')
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+ |{:SEQ_NAME=>"test1", :SEQ=>"atgcagcac", :SEQ_LEN=>9}
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+ |{:SEQ_NAME=>"test2", :SEQ=>"acagcactgA", :SEQ_LEN=>10}
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+ EXP
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+
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+ assert_equal(expected, collect_result)
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+ end
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+
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+ test 'BioDSL::Pipeline::ReadFasta with multiple files returns correctly' do
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+ @p.read_fasta(input: [@file, @file2]).run(output: @output2)
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+
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+ expected = <<-EXP.gsub(/^\s+\|/, '')
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+ |{:SEQ_NAME=>"test1", :SEQ=>"atgcagcac", :SEQ_LEN=>9}
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+ |{:SEQ_NAME=>"test2", :SEQ=>"acagcactgA", :SEQ_LEN=>10}
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+ |{:SEQ_NAME=>"test3", :SEQ=>"acGTAagcac", :SEQ_LEN=>10}
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+ |{:SEQ_NAME=>"test4", :SEQ=>"aCCAgcactgA", :SEQ_LEN=>11}
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+ EXP
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+
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+ assert_equal(expected, collect_result)
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+ end
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+
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+ test 'BioDSL::Pipeline::ReadFasta with input glob returns correctly' do
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+ @p.read_fasta(input: File.join(@tmpdir, 'test*.fna')).run(output: @output2)
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+
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+ expected = <<-EXP.gsub(/^\s+\|/, '')
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+ |{:SEQ_NAME=>"test1", :SEQ=>"atgcagcac", :SEQ_LEN=>9}
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+ |{:SEQ_NAME=>"test2", :SEQ=>"acagcactgA", :SEQ_LEN=>10}
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+ |{:SEQ_NAME=>"test3", :SEQ=>"acGTAagcac", :SEQ_LEN=>10}
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+ |{:SEQ_NAME=>"test4", :SEQ=>"aCCAgcactgA", :SEQ_LEN=>11}
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+ EXP
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+
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+ assert_equal(expected, collect_result)
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+ end
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+
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+ test 'BioDSL::Pipeline::ReadFasta with :first returns correctly' do
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+ @p.read_fasta(input: [@file, @file2], first: 3).run(output: @output2)
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+
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+ expected = <<-EXP.gsub(/^\s+\|/, '')
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+ |{:SEQ_NAME=>"test1", :SEQ=>"atgcagcac", :SEQ_LEN=>9}
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+ |{:SEQ_NAME=>"test2", :SEQ=>"acagcactgA", :SEQ_LEN=>10}
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+ |{:SEQ_NAME=>"test3", :SEQ=>"acGTAagcac", :SEQ_LEN=>10}
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+ EXP
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+
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+ assert_equal(expected, collect_result)
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+ end
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+
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+ test 'BioDSL::Pipeline::ReadFasta#to_s with :first returns correctly' do
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+ @p.read_fasta(input: @file, first: 3)
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+
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+ expected = %{BP.new.read_fasta(input: "#{@file}", first: 3)}
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+
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+ assert_equal(expected, @p.to_s)
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+ end
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+
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+ test 'BioDSL::Pipeline::ReadFasta with :last returns correctly' do
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+ @p.read_fasta(input: [@file, @file2], last: 3).run(output: @output2)
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+
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+ expected = <<-EXP.gsub(/^\s+\|/, '')
209
+ |{:SEQ_NAME=>"test2", :SEQ=>"acagcactgA", :SEQ_LEN=>10}
210
+ |{:SEQ_NAME=>"test3", :SEQ=>"acGTAagcac", :SEQ_LEN=>10}
211
+ |{:SEQ_NAME=>"test4", :SEQ=>"aCCAgcactgA", :SEQ_LEN=>11}
212
+ EXP
213
+
214
+ assert_equal(expected, collect_result)
215
+ end
216
+
217
+ test 'BioDSL::Pipeline::ReadFasta with flux returns correctly' do
218
+ @p.read_fasta(input: @file2).run(input: @input, output: @output2)
219
+
220
+ expected = <<-EXP.gsub(/^\s+\|/, '')
221
+ |{:SEQ_NAME=>"test1", :SEQ=>"atgcagcac", :SEQ_LEN=>9}
222
+ |{:SEQ_NAME=>"test2", :SEQ=>"acagcactgA", :SEQ_LEN=>10}
223
+ |{:SEQ_NAME=>"test3", :SEQ=>"acGTAagcac", :SEQ_LEN=>10}
224
+ |{:SEQ_NAME=>"test4", :SEQ=>"aCCAgcactgA", :SEQ_LEN=>11}
225
+ EXP
226
+
227
+ assert_equal(expected, collect_result)
228
+ end
229
+ end