BioDSL 1.0.0
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- checksums.yaml +7 -0
- data/.gitignore +10 -0
- data/BioDSL.gemspec +64 -0
- data/LICENSE +339 -0
- data/README.md +205 -0
- data/Rakefile +94 -0
- data/examples/fastq_to_fasta.rb +8 -0
- data/lib/BioDSL/cary.rb +242 -0
- data/lib/BioDSL/command.rb +133 -0
- data/lib/BioDSL/commands/add_key.rb +110 -0
- data/lib/BioDSL/commands/align_seq_mothur.rb +194 -0
- data/lib/BioDSL/commands/analyze_residue_distribution.rb +222 -0
- data/lib/BioDSL/commands/assemble_pairs.rb +336 -0
- data/lib/BioDSL/commands/assemble_seq_idba.rb +230 -0
- data/lib/BioDSL/commands/assemble_seq_ray.rb +345 -0
- data/lib/BioDSL/commands/assemble_seq_spades.rb +252 -0
- data/lib/BioDSL/commands/classify_seq.rb +217 -0
- data/lib/BioDSL/commands/classify_seq_mothur.rb +226 -0
- data/lib/BioDSL/commands/clip_primer.rb +318 -0
- data/lib/BioDSL/commands/cluster_otus.rb +181 -0
- data/lib/BioDSL/commands/collapse_otus.rb +170 -0
- data/lib/BioDSL/commands/collect_otus.rb +150 -0
- data/lib/BioDSL/commands/complement_seq.rb +117 -0
- data/lib/BioDSL/commands/count.rb +135 -0
- data/lib/BioDSL/commands/count_values.rb +149 -0
- data/lib/BioDSL/commands/degap_seq.rb +253 -0
- data/lib/BioDSL/commands/dereplicate_seq.rb +168 -0
- data/lib/BioDSL/commands/dump.rb +157 -0
- data/lib/BioDSL/commands/filter_rrna.rb +239 -0
- data/lib/BioDSL/commands/genecall.rb +237 -0
- data/lib/BioDSL/commands/grab.rb +535 -0
- data/lib/BioDSL/commands/index_taxonomy.rb +226 -0
- data/lib/BioDSL/commands/mask_seq.rb +175 -0
- data/lib/BioDSL/commands/mean_scores.rb +168 -0
- data/lib/BioDSL/commands/merge_pair_seq.rb +175 -0
- data/lib/BioDSL/commands/merge_table.rb +225 -0
- data/lib/BioDSL/commands/merge_values.rb +113 -0
- data/lib/BioDSL/commands/plot_heatmap.rb +233 -0
- data/lib/BioDSL/commands/plot_histogram.rb +306 -0
- data/lib/BioDSL/commands/plot_matches.rb +282 -0
- data/lib/BioDSL/commands/plot_residue_distribution.rb +278 -0
- data/lib/BioDSL/commands/plot_scores.rb +285 -0
- data/lib/BioDSL/commands/random.rb +153 -0
- data/lib/BioDSL/commands/read_fasta.rb +222 -0
- data/lib/BioDSL/commands/read_fastq.rb +414 -0
- data/lib/BioDSL/commands/read_table.rb +329 -0
- data/lib/BioDSL/commands/reverse_seq.rb +113 -0
- data/lib/BioDSL/commands/slice_align.rb +400 -0
- data/lib/BioDSL/commands/slice_seq.rb +151 -0
- data/lib/BioDSL/commands/sort.rb +223 -0
- data/lib/BioDSL/commands/split_pair_seq.rb +220 -0
- data/lib/BioDSL/commands/split_values.rb +165 -0
- data/lib/BioDSL/commands/trim_primer.rb +314 -0
- data/lib/BioDSL/commands/trim_seq.rb +192 -0
- data/lib/BioDSL/commands/uchime_ref.rb +170 -0
- data/lib/BioDSL/commands/uclust.rb +286 -0
- data/lib/BioDSL/commands/unique_values.rb +145 -0
- data/lib/BioDSL/commands/usearch_global.rb +171 -0
- data/lib/BioDSL/commands/usearch_local.rb +171 -0
- data/lib/BioDSL/commands/write_fasta.rb +207 -0
- data/lib/BioDSL/commands/write_fastq.rb +191 -0
- data/lib/BioDSL/commands/write_table.rb +419 -0
- data/lib/BioDSL/commands/write_tree.rb +167 -0
- data/lib/BioDSL/commands.rb +31 -0
- data/lib/BioDSL/config.rb +55 -0
- data/lib/BioDSL/csv.rb +307 -0
- data/lib/BioDSL/debug.rb +42 -0
- data/lib/BioDSL/fasta.rb +133 -0
- data/lib/BioDSL/fastq.rb +77 -0
- data/lib/BioDSL/filesys.rb +137 -0
- data/lib/BioDSL/fork.rb +145 -0
- data/lib/BioDSL/hamming.rb +128 -0
- data/lib/BioDSL/helpers/aux_helper.rb +44 -0
- data/lib/BioDSL/helpers/email_helper.rb +66 -0
- data/lib/BioDSL/helpers/history_helper.rb +40 -0
- data/lib/BioDSL/helpers/log_helper.rb +55 -0
- data/lib/BioDSL/helpers/options_helper.rb +405 -0
- data/lib/BioDSL/helpers/status_helper.rb +132 -0
- data/lib/BioDSL/helpers.rb +35 -0
- data/lib/BioDSL/html_report.rb +200 -0
- data/lib/BioDSL/math.rb +55 -0
- data/lib/BioDSL/mummer.rb +216 -0
- data/lib/BioDSL/pipeline.rb +354 -0
- data/lib/BioDSL/seq/ambiguity.rb +66 -0
- data/lib/BioDSL/seq/assemble.rb +240 -0
- data/lib/BioDSL/seq/backtrack.rb +252 -0
- data/lib/BioDSL/seq/digest.rb +99 -0
- data/lib/BioDSL/seq/dynamic.rb +263 -0
- data/lib/BioDSL/seq/homopolymer.rb +59 -0
- data/lib/BioDSL/seq/kmer.rb +293 -0
- data/lib/BioDSL/seq/levenshtein.rb +113 -0
- data/lib/BioDSL/seq/translate.rb +109 -0
- data/lib/BioDSL/seq/trim.rb +188 -0
- data/lib/BioDSL/seq.rb +742 -0
- data/lib/BioDSL/serializer.rb +98 -0
- data/lib/BioDSL/stream.rb +113 -0
- data/lib/BioDSL/taxonomy.rb +691 -0
- data/lib/BioDSL/test.rb +42 -0
- data/lib/BioDSL/tmp_dir.rb +68 -0
- data/lib/BioDSL/usearch.rb +301 -0
- data/lib/BioDSL/verbose.rb +42 -0
- data/lib/BioDSL/version.rb +31 -0
- data/lib/BioDSL.rb +81 -0
- data/test/BioDSL/commands/test_add_key.rb +105 -0
- data/test/BioDSL/commands/test_align_seq_mothur.rb +99 -0
- data/test/BioDSL/commands/test_analyze_residue_distribution.rb +134 -0
- data/test/BioDSL/commands/test_assemble_pairs.rb +459 -0
- data/test/BioDSL/commands/test_assemble_seq_idba.rb +50 -0
- data/test/BioDSL/commands/test_assemble_seq_ray.rb +51 -0
- data/test/BioDSL/commands/test_assemble_seq_spades.rb +50 -0
- data/test/BioDSL/commands/test_classify_seq.rb +50 -0
- data/test/BioDSL/commands/test_classify_seq_mothur.rb +59 -0
- data/test/BioDSL/commands/test_clip_primer.rb +377 -0
- data/test/BioDSL/commands/test_cluster_otus.rb +128 -0
- data/test/BioDSL/commands/test_collapse_otus.rb +81 -0
- data/test/BioDSL/commands/test_collect_otus.rb +82 -0
- data/test/BioDSL/commands/test_complement_seq.rb +78 -0
- data/test/BioDSL/commands/test_count.rb +103 -0
- data/test/BioDSL/commands/test_count_values.rb +85 -0
- data/test/BioDSL/commands/test_degap_seq.rb +96 -0
- data/test/BioDSL/commands/test_dereplicate_seq.rb +92 -0
- data/test/BioDSL/commands/test_dump.rb +109 -0
- data/test/BioDSL/commands/test_filter_rrna.rb +128 -0
- data/test/BioDSL/commands/test_genecall.rb +50 -0
- data/test/BioDSL/commands/test_grab.rb +398 -0
- data/test/BioDSL/commands/test_index_taxonomy.rb +62 -0
- data/test/BioDSL/commands/test_mask_seq.rb +98 -0
- data/test/BioDSL/commands/test_mean_scores.rb +111 -0
- data/test/BioDSL/commands/test_merge_pair_seq.rb +115 -0
- data/test/BioDSL/commands/test_merge_table.rb +131 -0
- data/test/BioDSL/commands/test_merge_values.rb +83 -0
- data/test/BioDSL/commands/test_plot_heatmap.rb +185 -0
- data/test/BioDSL/commands/test_plot_histogram.rb +194 -0
- data/test/BioDSL/commands/test_plot_matches.rb +157 -0
- data/test/BioDSL/commands/test_plot_residue_distribution.rb +309 -0
- data/test/BioDSL/commands/test_plot_scores.rb +308 -0
- data/test/BioDSL/commands/test_random.rb +88 -0
- data/test/BioDSL/commands/test_read_fasta.rb +229 -0
- data/test/BioDSL/commands/test_read_fastq.rb +552 -0
- data/test/BioDSL/commands/test_read_table.rb +327 -0
- data/test/BioDSL/commands/test_reverse_seq.rb +79 -0
- data/test/BioDSL/commands/test_slice_align.rb +218 -0
- data/test/BioDSL/commands/test_slice_seq.rb +131 -0
- data/test/BioDSL/commands/test_sort.rb +128 -0
- data/test/BioDSL/commands/test_split_pair_seq.rb +164 -0
- data/test/BioDSL/commands/test_split_values.rb +95 -0
- data/test/BioDSL/commands/test_trim_primer.rb +329 -0
- data/test/BioDSL/commands/test_trim_seq.rb +150 -0
- data/test/BioDSL/commands/test_uchime_ref.rb +113 -0
- data/test/BioDSL/commands/test_uclust.rb +139 -0
- data/test/BioDSL/commands/test_unique_values.rb +98 -0
- data/test/BioDSL/commands/test_usearch_global.rb +123 -0
- data/test/BioDSL/commands/test_usearch_local.rb +125 -0
- data/test/BioDSL/commands/test_write_fasta.rb +159 -0
- data/test/BioDSL/commands/test_write_fastq.rb +166 -0
- data/test/BioDSL/commands/test_write_table.rb +411 -0
- data/test/BioDSL/commands/test_write_tree.rb +122 -0
- data/test/BioDSL/helpers/test_options_helper.rb +272 -0
- data/test/BioDSL/seq/test_assemble.rb +98 -0
- data/test/BioDSL/seq/test_backtrack.rb +176 -0
- data/test/BioDSL/seq/test_digest.rb +71 -0
- data/test/BioDSL/seq/test_dynamic.rb +133 -0
- data/test/BioDSL/seq/test_homopolymer.rb +58 -0
- data/test/BioDSL/seq/test_kmer.rb +134 -0
- data/test/BioDSL/seq/test_translate.rb +75 -0
- data/test/BioDSL/seq/test_trim.rb +101 -0
- data/test/BioDSL/test_cary.rb +176 -0
- data/test/BioDSL/test_command.rb +45 -0
- data/test/BioDSL/test_csv.rb +514 -0
- data/test/BioDSL/test_debug.rb +42 -0
- data/test/BioDSL/test_fasta.rb +154 -0
- data/test/BioDSL/test_fastq.rb +46 -0
- data/test/BioDSL/test_filesys.rb +145 -0
- data/test/BioDSL/test_fork.rb +85 -0
- data/test/BioDSL/test_math.rb +41 -0
- data/test/BioDSL/test_mummer.rb +79 -0
- data/test/BioDSL/test_pipeline.rb +187 -0
- data/test/BioDSL/test_seq.rb +790 -0
- data/test/BioDSL/test_serializer.rb +72 -0
- data/test/BioDSL/test_stream.rb +55 -0
- data/test/BioDSL/test_taxonomy.rb +336 -0
- data/test/BioDSL/test_test.rb +42 -0
- data/test/BioDSL/test_tmp_dir.rb +58 -0
- data/test/BioDSL/test_usearch.rb +33 -0
- data/test/BioDSL/test_verbose.rb +42 -0
- data/test/helper.rb +82 -0
- data/www/command.html.haml +14 -0
- data/www/css.html.haml +55 -0
- data/www/input_files.html.haml +3 -0
- data/www/layout.html.haml +12 -0
- data/www/output_files.html.haml +3 -0
- data/www/overview.html.haml +15 -0
- data/www/pipeline.html.haml +4 -0
- data/www/png.html.haml +2 -0
- data/www/status.html.haml +9 -0
- data/www/time.html.haml +11 -0
- metadata +503 -0
data/lib/BioDSL/csv.rb
ADDED
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# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< #
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# #
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# Copyright (C) 2007-2015 Martin Asser Hansen (mail@maasha.dk). #
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# #
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# This program is free software; you can redistribute it and/or #
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# modify it under the terms of the GNU General Public License #
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# as published by the Free Software Foundation; either version 2 #
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# of the License, or (at your option) any later version. #
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# #
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# This program is distributed in the hope that it will be useful, #
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# but WITHOUT ANY WARRANTY; without even the implied warranty of #
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# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the #
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# GNU General Public License for more details. #
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# #
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# You should have received a copy of the GNU General Public License #
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# along with this program; if not, write to the Free Software #
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# Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, #
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# USA. #
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# #
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# http://www.gnu.org/copyleft/gpl.html #
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# #
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# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< #
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# #
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# This software is part of BioDSL (www.github.com/maasha/BioDSL). #
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# #
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# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< #
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# Monkey patching Array to add convert_types method.
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class Array
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# Method that converts variable types given an array of types.
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# Example: ["fish", 0.0, 1].convert_types([:to_s, :to_f, :to_i])
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def convert_types(types)
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if size != types.size
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fail ArgumentError, "Array and types size mismatch: #{size} != " \
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"#{types.size}"
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end
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types.each_with_index do |type, i|
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self[i] = self[i].send(type)
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end
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self
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end
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end
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module BioDSL
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class CSVError < StandardError; end
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# rubocop: disable ClassLength
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# Class for manipulating CSV or table files.
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# Allow reading and writing of gzip and bzip2 data.
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# Auto-convert data types.
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# Returns lines, arrays or hashes.
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class CSV
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def self.open(*args)
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io = IO.open(*args)
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if block_given?
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yield new(io)
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else
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return new(io)
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end
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end
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# Method that reads all CSV data from a file into an array of arrays (array
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# of rows) which is returned. In the default mode all columns are read.
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# Using the select option subselects the columns based on a given Array or
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# if a heder line is present a given Hash. Visa versa for the reject option.
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# Header lines are prefixed with '#' and are returned if the include_header
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# option is given.
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#
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# Options:
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# * include_header
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# * delimiter.
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# * select.
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# * reject.
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def self.read_array(file, options = {})
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data = []
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open(file) do |ios|
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ios.each_array(options) { |row| data << row }
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end
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data
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end
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# Method that reads all CSV data from a file into an array of hashes (array
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# of rows) which is returned. In the default mode all columns are read.
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# Using the select option subselects the columns based on a given Array or
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# if a heder line is present a given Hash. Visa versa for the reject option.
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# Header lines are prefixed with '#'.
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#
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# Options:
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# * delimiter.
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# * select.
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# * reject.
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def self.read_hash(file, options = {})
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data = []
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open(file) do |ios|
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ios.each_hash(options) { |row| data << row }
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end
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data
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end
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# Constructor method for CSV.
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def initialize(io)
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@io = io
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@delimiter = "\s"
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@header = nil
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@fields = nil
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@types = nil
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end
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# Method to skip a given number or non-empty lines.
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def skip(num)
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while num != 0 && (line = @io.gets)
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line.chomp!
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num -= 1 unless line.empty?
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end
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end
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# Method to iterate over a CSV IO object yielding arrays or an enumerator
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# CSV.each_array(options={}) { |item| block } -> ary
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# CSV.each_array(options={}) -> Enumerator
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#
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# Options:
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# * :include_header -
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# * :delimiter -
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# * :select -
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# * :reject -
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def each_array(options = {})
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return to_enum :each_array unless block_given?
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delimiter = options[:delimiter] || @delimiter
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@io.each do |line|
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line.chomp!
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next if line.empty?
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fields = line.split(delimiter)
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if line[0] == '#'
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get_header(fields, options) unless @header
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get_fields(fields, options) unless @fields
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yield @header.map(&:to_s) if options[:include_header]
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else
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get_header(fields, options) unless @header
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get_fields(fields, options) unless @fields
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fields = fields.values_at(*@fields) if @fields
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determine_types(fields) unless @types
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yield fields.convert_types(@types)
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end
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end
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self
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end
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# Method to iterate over a CSV IO object yielding hashes or an enumerator
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# CSV.each_hash(options={}) { |item| block } -> hash
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# CSV.each_hash(options={}) -> Enumerator
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#
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# Options:
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# * :delimiter -
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# * :select -
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# * :reject -
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def each_hash(options = {})
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each_array(options) do |array|
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hash = {}
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array.convert_types(@types).each_with_index do |field, i|
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hash[@header[i]] = field
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end
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yield hash
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end
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self
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186
|
+
end
|
187
|
+
|
188
|
+
private
|
189
|
+
|
190
|
+
# Method to set the @header given a list of fields (a row).
|
191
|
+
# Options:
|
192
|
+
# * :select - list of column indexes, names or a range to select.
|
193
|
+
# * :reject - list of column indexes, names or a range to reject.
|
194
|
+
def get_header(fields, options)
|
195
|
+
if fields[0][0] == '#'
|
196
|
+
fields[0] = fields[0][1..-1]
|
197
|
+
@header = fields.map(&:to_sym)
|
198
|
+
else
|
199
|
+
@header = []
|
200
|
+
fields.each_with_index { |_field, i| @header << "V#{i}".to_sym }
|
201
|
+
end
|
202
|
+
|
203
|
+
if options[:select]
|
204
|
+
if options[:select].first.is_a? Fixnum
|
205
|
+
if options[:select].max >= @header.size
|
206
|
+
fail CSVError, "Selected columns out of bounds: #{options[:select].
|
207
|
+
select { |c| c >= @header.size }}"
|
208
|
+
end
|
209
|
+
else
|
210
|
+
options[:select].each do |value|
|
211
|
+
unless @header.include? value.to_sym
|
212
|
+
fail CSVError, "Selected value: #{value} not in header: " \
|
213
|
+
" #{@header}"
|
214
|
+
end
|
215
|
+
end
|
216
|
+
end
|
217
|
+
elsif options[:reject]
|
218
|
+
if options[:reject].first.is_a? Fixnum
|
219
|
+
if options[:reject].max >= @header.size
|
220
|
+
fail CSVError, "Rejected columns out of bounds: #{options[:reject].
|
221
|
+
reject { |c| c >= @header.size }}"
|
222
|
+
end
|
223
|
+
else
|
224
|
+
options[:reject].map do |value|
|
225
|
+
unless @header.include? value.to_sym
|
226
|
+
fail CSVError, "Rejected value: #{value} not found in header: " \
|
227
|
+
"#{@header}"
|
228
|
+
end
|
229
|
+
end
|
230
|
+
end
|
231
|
+
end
|
232
|
+
|
233
|
+
@header
|
234
|
+
end
|
235
|
+
|
236
|
+
# Method to determine the indexes of fields to be parsed and store these in
|
237
|
+
# @fields.
|
238
|
+
# Options:
|
239
|
+
# * :select - list of column indexes, names or a range to select.
|
240
|
+
# * :reject - list of column indexes, names or a range to reject.
|
241
|
+
def get_fields(fields, options)
|
242
|
+
if options[:select]
|
243
|
+
if options[:select].first.is_a? Fixnum
|
244
|
+
@fields = options[:select]
|
245
|
+
else
|
246
|
+
fail CSVError, 'No header found' unless @header
|
247
|
+
|
248
|
+
fields = []
|
249
|
+
|
250
|
+
options[:select].each do |value|
|
251
|
+
fields << @header.index(value.to_sym)
|
252
|
+
end
|
253
|
+
|
254
|
+
@fields = fields
|
255
|
+
end
|
256
|
+
|
257
|
+
@header = @header.values_at(*@fields)
|
258
|
+
elsif options[:reject]
|
259
|
+
if options[:reject].first.is_a? Fixnum
|
260
|
+
reject = if options[:reject].is_a?(Range)
|
261
|
+
options[:reject].to_a
|
262
|
+
else
|
263
|
+
options[:reject]
|
264
|
+
end
|
265
|
+
@fields = (0...fields.size).to_a - reject
|
266
|
+
else
|
267
|
+
fail CSVError, 'No header found' unless @header
|
268
|
+
|
269
|
+
reject = options[:reject].map(&:to_sym)
|
270
|
+
|
271
|
+
@fields = @header.map.with_index.to_h.
|
272
|
+
delete_if { |k, _| reject.include? k }.values
|
273
|
+
end
|
274
|
+
|
275
|
+
@header = @header.values_at(*@fields)
|
276
|
+
end
|
277
|
+
end
|
278
|
+
|
279
|
+
# Method that determines the data types used in an array of fields.
|
280
|
+
def determine_types(fields)
|
281
|
+
types = []
|
282
|
+
|
283
|
+
fields.each do |field|
|
284
|
+
field = field.to_num
|
285
|
+
|
286
|
+
if field.is_a? Fixnum
|
287
|
+
types << :to_i
|
288
|
+
elsif field.is_a? Float
|
289
|
+
types << :to_f
|
290
|
+
elsif field.is_a? String
|
291
|
+
types << :to_s
|
292
|
+
else
|
293
|
+
types << nil
|
294
|
+
end
|
295
|
+
end
|
296
|
+
|
297
|
+
@types = types
|
298
|
+
end
|
299
|
+
|
300
|
+
# IO class for CSV.
|
301
|
+
class IO < Filesys
|
302
|
+
def gets
|
303
|
+
@io.gets
|
304
|
+
end
|
305
|
+
end
|
306
|
+
end
|
307
|
+
end
|
data/lib/BioDSL/debug.rb
ADDED
@@ -0,0 +1,42 @@
|
|
1
|
+
# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< #
|
2
|
+
# #
|
3
|
+
# Copyright (C) 2007-2015 Martin Asser Hansen (mail@maasha.dk). #
|
4
|
+
# #
|
5
|
+
# This program is free software; you can redistribute it and/or #
|
6
|
+
# modify it under the terms of the GNU General Public License #
|
7
|
+
# as published by the Free Software Foundation; either version 2 #
|
8
|
+
# of the License, or (at your option) any later version. #
|
9
|
+
# #
|
10
|
+
# This program is distributed in the hope that it will be useful, #
|
11
|
+
# but WITHOUT ANY WARRANTY; without even the implied warranty of #
|
12
|
+
# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the #
|
13
|
+
# GNU General Public License for more details. #
|
14
|
+
# #
|
15
|
+
# You should have received a copy of the GNU General Public License #
|
16
|
+
# along with this program; if not, write to the Free Software #
|
17
|
+
# Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, #
|
18
|
+
# USA. #
|
19
|
+
# #
|
20
|
+
# http://www.gnu.org/copyleft/gpl.html #
|
21
|
+
# #
|
22
|
+
# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< #
|
23
|
+
# #
|
24
|
+
# This software is part of the BioDSL framework (www.BioDSL.org). #
|
25
|
+
# #
|
26
|
+
# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< #
|
27
|
+
|
28
|
+
# Namespace for BioDSL.
|
29
|
+
module BioDSL
|
30
|
+
# Class variabel visible across the BioDSL module scope.
|
31
|
+
@@debug = false
|
32
|
+
|
33
|
+
# Class variable getter method.
|
34
|
+
def self.debug
|
35
|
+
@@debug
|
36
|
+
end
|
37
|
+
|
38
|
+
# Class variable setter method.
|
39
|
+
def self.debug=(x)
|
40
|
+
@@debug = x
|
41
|
+
end
|
42
|
+
end
|
data/lib/BioDSL/fasta.rb
ADDED
@@ -0,0 +1,133 @@
|
|
1
|
+
# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< #
|
2
|
+
# #
|
3
|
+
# Copyright (C) 2007-2015 Martin Asser Hansen (mail@maasha.dk). #
|
4
|
+
# #
|
5
|
+
# This program is free software; you can redistribute it and/or #
|
6
|
+
# modify it under the terms of the GNU General Public License #
|
7
|
+
# as published by the Free Software Foundation; either version 2 #
|
8
|
+
# of the License, or (at your option) any later version. #
|
9
|
+
# #
|
10
|
+
# This program is distributed in the hope that it will be useful, #
|
11
|
+
# but WITHOUT ANY WARRANTY; without even the implied warranty of #
|
12
|
+
# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the #
|
13
|
+
# GNU General Public License for more details. #
|
14
|
+
# #
|
15
|
+
# You should have received a copy of the GNU General Public License #
|
16
|
+
# along with this program; if not, write to the Free Software #
|
17
|
+
# Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA. #
|
18
|
+
# #
|
19
|
+
# http://www.gnu.org/copyleft/gpl.html #
|
20
|
+
# #
|
21
|
+
# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< #
|
22
|
+
# #
|
23
|
+
# This software is part of BioDSL (www.BioDSL.org). #
|
24
|
+
# #
|
25
|
+
# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< #
|
26
|
+
|
27
|
+
module BioDSL
|
28
|
+
# Error class for all exceptions to do with FASTA.
|
29
|
+
class FastaError < StandardError; end
|
30
|
+
|
31
|
+
class Fasta
|
32
|
+
def self.open(*args)
|
33
|
+
ios = IO.open(*args)
|
34
|
+
|
35
|
+
if block_given?
|
36
|
+
begin
|
37
|
+
yield self.new(ios)
|
38
|
+
ensure
|
39
|
+
ios.close
|
40
|
+
end
|
41
|
+
else
|
42
|
+
return self.new(ios)
|
43
|
+
end
|
44
|
+
end
|
45
|
+
|
46
|
+
def self.read(*args)
|
47
|
+
entries = []
|
48
|
+
|
49
|
+
Fasta.open(*args) do |ios|
|
50
|
+
ios.each do |entry|
|
51
|
+
entries << entry
|
52
|
+
end
|
53
|
+
end
|
54
|
+
|
55
|
+
entries
|
56
|
+
end
|
57
|
+
|
58
|
+
attr_accessor :seq_name, :seq
|
59
|
+
|
60
|
+
def initialize(io)
|
61
|
+
@io = io
|
62
|
+
@seq_name = nil
|
63
|
+
@seq = ""
|
64
|
+
@got_first = nil
|
65
|
+
@got_last = nil
|
66
|
+
end
|
67
|
+
|
68
|
+
def each
|
69
|
+
while entry = next_entry
|
70
|
+
yield entry
|
71
|
+
end
|
72
|
+
end
|
73
|
+
|
74
|
+
def puts(*args)
|
75
|
+
@io.puts(*args)
|
76
|
+
end
|
77
|
+
|
78
|
+
# Method to get the next FASTA entry form an ios and return this
|
79
|
+
# as a Seq object. If no entry is found or eof then nil is returned.
|
80
|
+
def next_entry
|
81
|
+
@io.each do |line|
|
82
|
+
line.chomp!
|
83
|
+
|
84
|
+
next if line.empty?
|
85
|
+
|
86
|
+
if line[0] == '>'
|
87
|
+
if not @got_first and not @seq.empty?
|
88
|
+
raise FastaError, "Bad FASTA format -> content before Fasta header: #{@seq}" unless @seq.empty?
|
89
|
+
end
|
90
|
+
|
91
|
+
@got_first = true
|
92
|
+
|
93
|
+
if @seq_name
|
94
|
+
entry = Seq.new(seq_name: @seq_name, seq: @seq)
|
95
|
+
@seq_name = line[1 .. -1]
|
96
|
+
@seq = ""
|
97
|
+
|
98
|
+
raise FastaError, "Bad FASTA format -> truncated Fasta header: no content after '>'" if @seq_name.empty?
|
99
|
+
|
100
|
+
return entry
|
101
|
+
else
|
102
|
+
@seq_name = line[1 .. -1]
|
103
|
+
|
104
|
+
raise FastaError, "Bad FASTA format -> truncated Fasta header: no content after '>'" if @seq_name.empty?
|
105
|
+
end
|
106
|
+
else
|
107
|
+
@seq << line
|
108
|
+
end
|
109
|
+
end
|
110
|
+
|
111
|
+
if @seq_name
|
112
|
+
@got_last = true
|
113
|
+
entry = Seq.new(seq_name: @seq_name, seq: @seq)
|
114
|
+
@seq_name = nil
|
115
|
+
return entry
|
116
|
+
end
|
117
|
+
|
118
|
+
if not @got_last and not @seq.empty?
|
119
|
+
raise FastaError, "Bad FASTA format -> content witout Fasta header: #{@seq}"
|
120
|
+
end
|
121
|
+
|
122
|
+
nil
|
123
|
+
end
|
124
|
+
|
125
|
+
class IO < Filesys
|
126
|
+
def each
|
127
|
+
while not @io.eof?
|
128
|
+
yield @io.gets
|
129
|
+
end
|
130
|
+
end
|
131
|
+
end
|
132
|
+
end
|
133
|
+
end
|
data/lib/BioDSL/fastq.rb
ADDED
@@ -0,0 +1,77 @@
|
|
1
|
+
# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< #
|
2
|
+
# #
|
3
|
+
# Copyright (C) 2007-2015 Martin Asser Hansen (mail@maasha.dk). #
|
4
|
+
# #
|
5
|
+
# This program is free software; you can redistribute it and/or #
|
6
|
+
# modify it under the terms of the GNU General Public License #
|
7
|
+
# as published by the Free Software Foundation; either version 2 #
|
8
|
+
# of the License, or (at your option) any later version. #
|
9
|
+
# #
|
10
|
+
# This program is distributed in the hope that it will be useful, #
|
11
|
+
# but WITHOUT ANY WARRANTY; without even the implied warranty of #
|
12
|
+
# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the #
|
13
|
+
# GNU General Public License for more details. #
|
14
|
+
# #
|
15
|
+
# You should have received a copy of the GNU General Public License #
|
16
|
+
# along with this program; if not, write to the Free Software #
|
17
|
+
# Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA. #
|
18
|
+
# #
|
19
|
+
# http://www.gnu.org/copyleft/gpl.html #
|
20
|
+
# #
|
21
|
+
# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< #
|
22
|
+
# #
|
23
|
+
# This software is part of BioDSL (www.BioDSL.org). #
|
24
|
+
# #
|
25
|
+
# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< #
|
26
|
+
|
27
|
+
module BioDSL
|
28
|
+
# Error class for all exceptions to do with FASTQ.
|
29
|
+
class FastqError < StandardError; end
|
30
|
+
|
31
|
+
# Class for parsing FASTQ entries from an ios and return as Seq objects.
|
32
|
+
class Fastq < BioDSL::Filesys
|
33
|
+
def self.open(*args)
|
34
|
+
ios = IO.open(*args)
|
35
|
+
|
36
|
+
if block_given?
|
37
|
+
begin
|
38
|
+
yield self.new(ios)
|
39
|
+
ensure
|
40
|
+
ios.close
|
41
|
+
end
|
42
|
+
else
|
43
|
+
return self.new(ios)
|
44
|
+
end
|
45
|
+
end
|
46
|
+
|
47
|
+
def initialize(io)
|
48
|
+
@io = io
|
49
|
+
end
|
50
|
+
|
51
|
+
def each
|
52
|
+
while entry = next_entry
|
53
|
+
yield entry
|
54
|
+
end
|
55
|
+
end
|
56
|
+
|
57
|
+
# Method to get the next FASTQ entry from an ios and return this
|
58
|
+
# as a Seq object. If no entry is found or eof then nil is returned.
|
59
|
+
def next_entry
|
60
|
+
return nil if @io.eof?
|
61
|
+
seq_name = @io.gets[1 .. -2]
|
62
|
+
seq = @io.gets.chomp
|
63
|
+
@io.gets
|
64
|
+
qual = @io.gets.chomp
|
65
|
+
|
66
|
+
Seq.new(seq_name: seq_name, seq: seq, qual: qual)
|
67
|
+
end
|
68
|
+
|
69
|
+
class IO < Filesys
|
70
|
+
def each
|
71
|
+
while not @io.eof?
|
72
|
+
yield @io.gets
|
73
|
+
end
|
74
|
+
end
|
75
|
+
end
|
76
|
+
end
|
77
|
+
end
|
@@ -0,0 +1,137 @@
|
|
1
|
+
# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< #
|
2
|
+
# #
|
3
|
+
# Copyright (C) 2007-2015 Martin Asser Hansen (mail@maasha.dk). #
|
4
|
+
# #
|
5
|
+
# This program is free software; you can redistribute it and/or #
|
6
|
+
# modify it under the terms of the GNU General Public License #
|
7
|
+
# as published by the Free Software Foundation; either version 2 #
|
8
|
+
# of the License, or (at your option) any later version. #
|
9
|
+
# #
|
10
|
+
# This program is distributed in the hope that it will be useful, #
|
11
|
+
# but WITHOUT ANY WARRANTY; without even the implied warranty of #
|
12
|
+
# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the #
|
13
|
+
# GNU General Public License for more details. #
|
14
|
+
# #
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# You should have received a copy of the GNU General Public License #
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# along with this program; if not, write to the Free Software #
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# Foundation, Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301, USA. #
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# #
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# http://www.gnu.org/copyleft/gpl.html #
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# #
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# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< #
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# #
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# This software is part of BioDSL (www.BioDSL.org). #
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# #
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# >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>><<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<< #
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module BioDSL
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# Error class for all exceptions to do with Filesys.
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class FilesysError < StandardError; end
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+
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class Filesys
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require 'open3'
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+
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include Enumerable
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+
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# Cross-platform way of finding an executable in the $PATH.
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#
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# which('ruby') #=> /usr/bin/ruby
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def self.which(cmd)
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exts = ENV['PATHEXT'] ? ENV['PATHEXT'].split(';') : ['']
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+
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ENV['PATH'].split(File::PATH_SEPARATOR).each do |path|
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exts.each { |ext|
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exe = File.join(path, "#{cmd}#{ext}")
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return exe if File.executable?(exe) && !File.directory?(exe)
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+
}
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+
end
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48
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+
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49
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+
nil
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+
end
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51
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+
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52
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+
# Class method that returns a path to a unique temporary file.
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+
# If no directory is specified reverts to the systems tmp directory.
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54
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+
def self.tmpfile(tmp_dir = ENV["TMPDIR"])
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+
time = Time.now.to_i
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user = ENV["USER"]
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pid = $$
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path = tmp_dir + [user, time + pid, pid].join("_") + ".tmp"
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+
path
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+
end
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61
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+
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62
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+
def self.open(*args)
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+
file = args.shift
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+
mode = args.shift
|
65
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+
options = args.shift || {}
|
66
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+
|
67
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+
if mode == 'w'
|
68
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+
case options[:compress]
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69
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+
when :gzip
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+
ios, = Open3.pipeline_w("gzip -f", out: file)
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71
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+
when :bzip, :bzip2
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ios, = Open3.pipeline_w("bzip2 -c", out: file)
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else
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ios = File.open(file, mode, options)
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+
end
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76
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else
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type = (file.respond_to? :path) ? `file -Lk #{file.path}` : `file -Lk #{file}`
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+
case type
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when /gzip/
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ios = IO.popen("gzip -cd #{file}")
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+
when /bzip/
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ios = IO.popen("bzcat #{file}")
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else
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84
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+
ios = File.open(file, mode, options)
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85
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+
end
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86
|
+
end
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87
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+
|
88
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+
if block_given?
|
89
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+
begin
|
90
|
+
yield self.new(ios)
|
91
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+
ensure
|
92
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+
ios.close
|
93
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+
end
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94
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+
else
|
95
|
+
return self.new(ios)
|
96
|
+
end
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97
|
+
end
|
98
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+
|
99
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+
attr_reader :io
|
100
|
+
|
101
|
+
def initialize(ios)
|
102
|
+
@io = ios
|
103
|
+
end
|
104
|
+
|
105
|
+
def gets
|
106
|
+
@io.gets
|
107
|
+
end
|
108
|
+
|
109
|
+
def puts(*args)
|
110
|
+
@io.puts(*args)
|
111
|
+
end
|
112
|
+
|
113
|
+
def read
|
114
|
+
@io.read
|
115
|
+
end
|
116
|
+
|
117
|
+
def write(arg)
|
118
|
+
@io.write arg
|
119
|
+
end
|
120
|
+
|
121
|
+
def close
|
122
|
+
@io.close
|
123
|
+
end
|
124
|
+
|
125
|
+
def eof?
|
126
|
+
@io.eof?
|
127
|
+
end
|
128
|
+
|
129
|
+
# Iterator method for parsing entries.
|
130
|
+
def each
|
131
|
+
return to_enum :each unless block_given?
|
132
|
+
|
133
|
+
@io.each { |line| yield line }
|
134
|
+
end
|
135
|
+
end
|
136
|
+
end
|
137
|
+
|