minimap2 0.2.22.0 → 0.2.24.1

data/ext/minimap2/minimap.h

@@ -0,0 +1,410 @@
+#ifndef MINIMAP2_H
+#define MINIMAP2_H
+
+#include <stdint.h>
+#include <stdio.h>
+#include <sys/types.h>
+
+#define MM_F_NO_DIAG       0x001 // no exact diagonal hit
+#define MM_F_NO_DUAL       0x002 // skip pairs where query name is lexicographically larger than target name
+#define MM_F_CIGAR         0x004
+#define MM_F_OUT_SAM       0x008
+#define MM_F_NO_QUAL       0x010
+#define MM_F_OUT_CG        0x020
+#define MM_F_OUT_CS        0x040
+#define MM_F_SPLICE        0x080 // splice mode
+#define MM_F_SPLICE_FOR    0x100 // match GT-AG
+#define MM_F_SPLICE_REV    0x200 // match CT-AC, the reverse complement of GT-AG
+#define MM_F_NO_LJOIN      0x400
+#define MM_F_OUT_CS_LONG   0x800
+#define MM_F_SR            0x1000
+#define MM_F_FRAG_MODE     0x2000
+#define MM_F_NO_PRINT_2ND  0x4000
+#define MM_F_2_IO_THREADS  0x8000
+#define MM_F_LONG_CIGAR    0x10000
+#define MM_F_INDEPEND_SEG  0x20000
+#define MM_F_SPLICE_FLANK  0x40000
+#define MM_F_SOFTCLIP      0x80000
+#define MM_F_FOR_ONLY      0x100000
+#define MM_F_REV_ONLY      0x200000
+#define MM_F_HEAP_SORT     0x400000
+#define MM_F_ALL_CHAINS    0x800000
+#define MM_F_OUT_MD        0x1000000
+#define MM_F_COPY_COMMENT  0x2000000
+#define MM_F_EQX           0x4000000 // use =/X instead of M
+#define MM_F_PAF_NO_HIT    0x8000000 // output unmapped reads to PAF
+#define MM_F_NO_END_FLT    0x10000000
+#define MM_F_HARD_MLEVEL   0x20000000
+#define MM_F_SAM_HIT_ONLY  0x40000000
+#define MM_F_RMQ           (0x80000000LL)
+#define MM_F_QSTRAND       (0x100000000LL)
+#define MM_F_NO_INV        (0x200000000LL)
+#define MM_F_NO_HASH_NAME  (0x400000000LL)
+
+#define MM_I_HPC          0x1
+#define MM_I_NO_SEQ       0x2
+#define MM_I_NO_NAME      0x4
+
+#define MM_IDX_MAGIC   "MMI\2"
+
+#define MM_MAX_SEG       255
+
+#define MM_CIGAR_MATCH      0
+#define MM_CIGAR_INS        1
+#define MM_CIGAR_DEL        2
+#define MM_CIGAR_N_SKIP     3
+#define MM_CIGAR_SOFTCLIP   4
+#define MM_CIGAR_HARDCLIP   5
+#define MM_CIGAR_PADDING    6
+#define MM_CIGAR_EQ_MATCH   7
+#define MM_CIGAR_X_MISMATCH 8
+
+#define MM_CIGAR_STR  "MIDNSHP=XB"
+
+#ifdef __cplusplus
+extern "C" {
+#endif
+
+// emulate 128-bit integers and arrays
+typedef struct { uint64_t x, y; } mm128_t;
+typedef struct { size_t n, m; mm128_t *a; } mm128_v;
+
+// minimap2 index
+typedef struct {
+	char *name;      // name of the db sequence
+	uint64_t offset; // offset in mm_idx_t::S
+	uint32_t len;    // length
+	uint32_t is_alt;
+} mm_idx_seq_t;
+
+typedef struct {
+	int32_t b, w, k, flag;
+	uint32_t n_seq;            // number of reference sequences
+	int32_t index;
+	int32_t n_alt;
+	mm_idx_seq_t *seq;         // sequence name, length and offset
+	uint32_t *S;               // 4-bit packed sequence
+	struct mm_idx_bucket_s *B; // index (hidden)
+	struct mm_idx_intv_s *I;   // intervals (hidden)
+	void *km, *h;
+} mm_idx_t;
+
+// minimap2 alignment
+typedef struct {
+	uint32_t capacity;                  // the capacity of cigar[]
+	int32_t dp_score, dp_max, dp_max2;  // DP score; score of the max-scoring segment; score of the best alternate mappings
+	uint32_t n_ambi:30, trans_strand:2; // number of ambiguous bases; transcript strand: 0 for unknown, 1 for +, 2 for -
+	uint32_t n_cigar;                   // number of cigar operations in cigar[]
+	uint32_t cigar[];
+} mm_extra_t;
+
+typedef struct {
+	int32_t id;             // ID for internal uses (see also parent below)
+	int32_t cnt;            // number of minimizers; if on the reverse strand
+	int32_t rid;            // reference index; if this is an alignment from inversion rescue
+	int32_t score;          // DP alignment score
+	int32_t qs, qe, rs, re; // query start and end; reference start and end
+	int32_t parent, subsc;  // parent==id if primary; best alternate mapping score
+	int32_t as;             // offset in the a[] array (for internal uses only)
+	int32_t mlen, blen;     // seeded exact match length; seeded alignment block length
+	int32_t n_sub;          // number of suboptimal mappings
+	int32_t score0;         // initial chaining score (before chain merging/spliting)
+	uint32_t mapq:8, split:2, rev:1, inv:1, sam_pri:1, proper_frag:1, pe_thru:1, seg_split:1, seg_id:8, split_inv:1, is_alt:1, strand_retained:1, dummy:5;
+	uint32_t hash;
+	float div;
+	mm_extra_t *p;
+} mm_reg1_t;
+
+// indexing and mapping options
+typedef struct {
+	short k, w, flag, bucket_bits;
+	int64_t mini_batch_size;
+	uint64_t batch_size;
+} mm_idxopt_t;
+
+typedef struct {
+	int64_t flag;    // see MM_F_* macros
+	int seed;
+	int sdust_thres; // score threshold for SDUST; 0 to disable
+
+	int max_qlen;    // max query length
+
+	int bw, bw_long; // bandwidth
+	int max_gap, max_gap_ref; // break a chain if there are no minimizers in a max_gap window
+	int max_frag_len;
+	int max_chain_skip, max_chain_iter;
+	int min_cnt;         // min number of minimizers on each chain
+	int min_chain_score; // min chaining score
+	float chain_gap_scale;
+	float chain_skip_scale;
+	int rmq_size_cap, rmq_inner_dist;
+	int rmq_rescue_size;
+	float rmq_rescue_ratio;
+
+	float mask_level;
+	int mask_len;
+	float pri_ratio;
+	int best_n;      // top best_n chains are subjected to DP alignment
+
+	float alt_drop;
+
+	int a, b, q, e, q2, e2; // matching score, mismatch, gap-open and gap-ext penalties
+	int sc_ambi; // score when one or both bases are "N"
+	int noncan;      // cost of non-canonical splicing sites
+	int junc_bonus;
+	int zdrop, zdrop_inv;   // break alignment if alignment score drops too fast along the diagonal
+	int end_bonus;
+	int min_dp_max;  // drop an alignment if the score of the max scoring segment is below this threshold
+	int min_ksw_len;
+	int anchor_ext_len, anchor_ext_shift;
+	float max_clip_ratio; // drop an alignment if BOTH ends are clipped above this ratio
+
+	int rank_min_len;
+	float rank_frac;
+
+	int pe_ori, pe_bonus;
+
+	float mid_occ_frac;  // only used by mm_mapopt_update(); see below
+	float q_occ_frac;
+	int32_t min_mid_occ, max_mid_occ;
+	int32_t mid_occ;     // ignore seeds with occurrences above this threshold
+	int32_t max_occ, max_max_occ, occ_dist;
+	int64_t mini_batch_size; // size of a batch of query bases to process in parallel
+	int64_t max_sw_mat;
+	int64_t cap_kalloc;
+
+	const char *split_prefix;
+} mm_mapopt_t;
+
+// index reader
+typedef struct {
+	int is_idx, n_parts;
+	int64_t idx_size;
+	mm_idxopt_t opt;
+	FILE *fp_out;
+	union {
+		struct mm_bseq_file_s *seq;
+		FILE *idx;
+	} fp;
+} mm_idx_reader_t;
+
+// memory buffer for thread-local storage during mapping
+typedef struct mm_tbuf_s mm_tbuf_t;
+
+// global variables
+extern int mm_verbose, mm_dbg_flag; // verbose level: 0 for no info, 1 for error, 2 for warning, 3 for message (default); debugging flag
+extern double mm_realtime0; // wall-clock timer
+
+/**
+ * Set default or preset parameters
+ *
+ * @param preset     NULL to set all parameters as default; otherwise apply preset to affected parameters
+ * @param io         pointer to indexing parameters
+ * @param mo         pointer to mapping parameters
+ *
+ * @return 0 if success; -1 if _present_ unknown
+ */
+int mm_set_opt(const char *preset, mm_idxopt_t *io, mm_mapopt_t *mo);
+int mm_check_opt(const mm_idxopt_t *io, const mm_mapopt_t *mo);
+
+/**
+ * Update mm_mapopt_t::mid_occ via mm_mapopt_t::mid_occ_frac
+ *
+ * If mm_mapopt_t::mid_occ is 0, this function sets it to a number such that no
+ * more than mm_mapopt_t::mid_occ_frac of minimizers in the index have a higher
+ * occurrence.
+ *
+ * @param opt        mapping parameters
+ * @param mi         minimap2 index
+ */
+void mm_mapopt_update(mm_mapopt_t *opt, const mm_idx_t *mi);
+
+void mm_mapopt_max_intron_len(mm_mapopt_t *opt, int max_intron_len);
+
+/**
+ * Initialize an index reader
+ *
+ * @param fn         index or fasta/fastq file name (this function tests the file type)
+ * @param opt        indexing parameters
+ * @param fn_out     if not NULL, write built index to this file
+ *
+ * @return an index reader on success; NULL if fail to open _fn_
+ */
+mm_idx_reader_t *mm_idx_reader_open(const char *fn, const mm_idxopt_t *opt, const char *fn_out);
+
+/**
+ * Read/build an index
+ *
+ * If the input file is an index file, this function reads one part of the
+ * index and returns. If the input file is a sequence file (fasta or fastq),
+ * this function constructs the index for about mm_idxopt_t::batch_size bases.
+ * Importantly, for a huge collection of sequences, this function may only
+ * return an index for part of sequences. It needs to be repeatedly called
+ * to traverse the entire index/sequence file.
+ *
+ * @param r          index reader
+ * @param n_threads  number of threads for constructing index
+ *
+ * @return an index on success; NULL if reaching the end of the input file
+ */
+mm_idx_t *mm_idx_reader_read(mm_idx_reader_t *r, int n_threads);
+
+/**
+ * Destroy/deallocate an index reader
+ *
+ * @param r          index reader
+ */
+void mm_idx_reader_close(mm_idx_reader_t *r);
+
+int mm_idx_reader_eof(const mm_idx_reader_t *r);
+
+/**
+ * Check whether the file contains a minimap2 index
+ *
+ * @param fn         file name
+ *
+ * @return the file size if fn is an index file; 0 if fn is not.
+ */
+int64_t mm_idx_is_idx(const char *fn);
+
+/**
+ * Load a part of an index
+ *
+ * Given a uni-part index, this function loads the entire index into memory.
+ * Given a multi-part index, it loads one part only and places the file pointer
+ * at the end of that part.
+ *
+ * @param fp         pointer to FILE object
+ *
+ * @return minimap2 index read from fp
+ */
+mm_idx_t *mm_idx_load(FILE *fp);
+
+/**
+ * Append an index (or one part of a full index) to file
+ *
+ * @param fp         pointer to FILE object
+ * @param mi         minimap2 index
+ */
+void mm_idx_dump(FILE *fp, const mm_idx_t *mi);
+
+/**
+ * Create an index from strings in memory
+ *
+ * @param w            minimizer window size
+ * @param k            minimizer k-mer size
+ * @param is_hpc       use HPC k-mer if true
+ * @param bucket_bits  number of bits for the first level of the hash table
+ * @param n            number of sequences
+ * @param seq          sequences in A/C/G/T
+ * @param name         sequence names; could be NULL
+ *
+ * @return minimap2 index
+ */
+mm_idx_t *mm_idx_str(int w, int k, int is_hpc, int bucket_bits, int n, const char **seq, const char **name);
+
+/**
+ * Print index statistics to stderr
+ *
+ * @param mi         minimap2 index
+ */
+void mm_idx_stat(const mm_idx_t *idx);
+
+/**
+ * Destroy/deallocate an index
+ *
+ * @param r          minimap2 index
+ */
+void mm_idx_destroy(mm_idx_t *mi);
+
+/**
+ * Initialize a thread-local buffer for mapping
+ *
+ * Each mapping thread requires a buffer specific to the thread (see mm_map()
+ * below). The primary purpose of this buffer is to reduce frequent heap
+ * allocations across threads. A buffer shall not be used by two or more
+ * threads.
+ *
+ * @return pointer to a thread-local buffer
+ */
+mm_tbuf_t *mm_tbuf_init(void);
+
+/**
+ * Destroy/deallocate a thread-local buffer for mapping
+ *
+ * @param b          the buffer
+ */
+void mm_tbuf_destroy(mm_tbuf_t *b);
+
+void *mm_tbuf_get_km(mm_tbuf_t *b);
+
+/**
+ * Align a query sequence against an index
+ *
+ * This function possibly finds multiple alignments of the query sequence.
+ * The returned array and the mm_reg1_t::p field of each element are allocated
+ * with malloc().
+ *
+ * @param mi         minimap2 index
+ * @param l_seq      length of the query sequence
+ * @param seq        the query sequence
+ * @param n_regs     number of hits (out)
+ * @param b          thread-local buffer; two mm_map() calls shall not use one buffer at the same time!
+ * @param opt        mapping parameters
+ * @param name       query name, used for all-vs-all overlapping and debugging
+ *
+ * @return an array of hits which need to be deallocated with free() together
+ *         with mm_reg1_t::p of each element. The size is written to _n_regs_.
+ */
+mm_reg1_t *mm_map(const mm_idx_t *mi, int l_seq, const char *seq, int *n_regs, mm_tbuf_t *b, const mm_mapopt_t *opt, const char *name);
+
+void mm_map_frag(const mm_idx_t *mi, int n_segs, const int *qlens, const char **seqs, int *n_regs, mm_reg1_t **regs, mm_tbuf_t *b, const mm_mapopt_t *opt, const char *qname);
+
+/**
+ * Align a fasta/fastq file and print alignments to stdout
+ *
+ * @param idx        minimap2 index
+ * @param fn         fasta/fastq file name
+ * @param opt        mapping parameters
+ * @param n_threads  number of threads
+ *
+ * @return 0 on success; -1 if _fn_ can't be read
+ */
+int mm_map_file(const mm_idx_t *idx, const char *fn, const mm_mapopt_t *opt, int n_threads);
+
+int mm_map_file_frag(const mm_idx_t *idx, int n_segs, const char **fn, const mm_mapopt_t *opt, int n_threads);
+
+/**
+ * Generate the cs tag (new in 2.12)
+ *
+ * @param km         memory blocks; set to NULL if unsure
+ * @param buf        buffer to write the cs/MD tag; typicall NULL on the first call
+ * @param max_len    max length of the buffer; typically set to 0 on the first call
+ * @param mi         index
+ * @param r          alignment
+ * @param seq        query sequence
+ * @param no_iden    true to use : instead of =
+ *
+ * @return the length of cs
+ */
+int mm_gen_cs(void *km, char **buf, int *max_len, const mm_idx_t *mi, const mm_reg1_t *r, const char *seq, int no_iden);
+int mm_gen_MD(void *km, char **buf, int *max_len, const mm_idx_t *mi, const mm_reg1_t *r, const char *seq);
+
+// query sequence name and sequence in the minimap2 index
+int mm_idx_index_name(mm_idx_t *mi);
+int mm_idx_name2id(const mm_idx_t *mi, const char *name);
+int mm_idx_getseq(const mm_idx_t *mi, uint32_t rid, uint32_t st, uint32_t en, uint8_t *seq);
+
+int mm_idx_alt_read(mm_idx_t *mi, const char *fn);
+int mm_idx_bed_read(mm_idx_t *mi, const char *fn, int read_junc);
+int mm_idx_bed_junc(const mm_idx_t *mi, int32_t ctg, int32_t st, int32_t en, uint8_t *s);
+
+// deprecated APIs for backward compatibility
+void mm_mapopt_init(mm_mapopt_t *opt);
+mm_idx_t *mm_idx_build(const char *fn, int w, int k, int flag, int n_threads);
+
+#ifdef __cplusplus
+}
+#endif
+
+#endif // MINIMAP2_H