MARQ 0.0.1

data/install_scripts/GEO/series/GSE6018.yaml

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+--- 
+:description: |-
+  Wt S. cerevisiae cells from a W303 background were exposed to multiple doses of MMS and Gamma Radiation and their global transcriptional response was measured
+  Keywords: dose response
+:title: Analyzing the dose-dependence of the Saccharomyces cerevisiae global transcriptional response to MMS and IR
+:arrays: 
+  GSM139754: 
+    condition: IR 1
+  GSM139743: 
+    condition: MMS 0.01
+  GSM139755: 
+    condition: IR 10
+  GSM139744: 
+    condition: MMS 0.1
+  GSM139733: 
+    condition: MMS 0
+  GSM139756: 
+    condition: IR 100
+  GSM139745: 
+    condition: IR 0
+  GSM139734: 
+    condition: MMS 0.001
+  GSM139746: 
+    condition: IR 1
+  GSM139735: 
+    condition: MMS 0.01
+  GSM139747: 
+    condition: IR 10
+  GSM139736: 
+    condition: MMS 0.1
+  GSM139748: 
+    condition: IR 100
+  GSM139737: 
+    condition: MMS 0
+  GSM139750: 
+    condition: IR 1
+  GSM139749: 
+    condition: IR 0
+  GSM139738: 
+    condition: MMS 0.001
+  GSM139751: 
+    condition: IR 10
+  GSM139740: 
+    condition: MMS 0.1
+  GSM139739: 
+    condition: MMS 0.01
+  GSM139752: 
+    condition: IR 100
+  GSM139741: 
+    condition: MMS 0
+  GSM139753: 
+    condition: IR 0
+  GSM139742: 
+    condition: MMS 0.001
+:platform: GPL4416

data/install_scripts/GEO/series/GSE6066.yaml

@@ -0,0 +1,20 @@
+--- 
+:description: |-
+  Arsenic is ubiquitously present in nature and various mechanisms have evolved enabling cells to evade toxicity and acquire tolerance. Herein, we explored how Saccharomyces cerevisiae (budding yeast) respond to trivalent arsenic (arsenite) by quantitative and kinetic transcriptome, proteome and sulfur metabolite profiling. Arsenite exposure affected transcription of genes encoding functions related to protein biosynthesis, arsenic detoxification, oxidative stress defense, redox maintenance and
+  proteolytic activity. Importantly, enzymes involved in sulfate assimilation and glutathione biosynthesis were induced at both gene and protein levels. Kinetic metabolic profiling evidenced a significant increase in the pools of sulfur metabolites as well as elevated glutathione levels. Moreover, the flux in the sulfur assimilation pathway as well as the glutathione synthesis rate strongly increased with a concomitant reduction of sulfur incorporation into proteins. By combining comparative genomics and molecular analyses, we pin-pointed transcription factors that mediate thecore of the transcriptional response to arsenite. Taken together, our data reveals that arsenite-exposed cells channel a large part of assimilated sulfur into glutathione biosynthesis and we provide evidence that the transcriptional regulators Yap1p and Met4p control this response in concert.
+  Keywords: stress
+:arrays: 
+  GSM140765: 
+    treatment: As stress
+  GSM140767: 
+    treatment: As stress
+  GSM140768: 
+    treatment: As stress
+  GSM140769: 
+    treatment: As stress
+  GSM140770: 
+    treatment: As stress
+  GSM140762: 
+    treatment: As stress
+:title: 1.0 mM As(III) stressed wild-type vs (non-stressed) wild-type after 1 hour
+:platform: GPL4462

data/install_scripts/GEO/series/GSE6067.yaml

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+--- 
+:description: |-
+  Arsenic is ubiquitously present in nature and various mechanisms have
+  evolved enabling cells to evade toxicity and acquire tolerance. Herein, we
+  explored how Saccharomyces cerevisiae (budding yeast) respond to trivalent
+  arsenic (arsenite) by quantitative and kinetic transcriptome, proteome and
+  sulfur metabolite profiling. Arsenite exposure affected transcription of
+  genes encoding functions related to protein biosynthesis, arsenic
+  detoxification, oxidative stress defense, redox maintenance and
+  proteolytic activity. Importantly, enzymes involved in sulfate assimilation
+  and glutathione biosynthesis were induced at both gene and protein levels.
+  Kinetic metabolic profiling evidenced a significant increase in the pools of
+  sulfur metabolites as well as elevated glutathione levels. Moreover, the
+  flux in the sulfur assimilation pathway as well as the glutathione synthesis
+  rate strongly increased with a concomitant reduction of sulfur incorporation
+  into proteins. By combining comparative genomics and molecular analyses, we
+  pin-pointed transcription factors that mediate thecore of the
+  transcriptional response to arsenite. Taken together, our data reveals that
+  arsenite-exposed cells channel a large part of assimilated sulfur into
+  glutathione biosynthesis and we provide evidence that the transcriptional
+  regulators Yap1p and Met4p control this response in concert.
+  Keywords: stress
+:arrays: 
+  GSM140771: 
+    treatment: AS Stress 0.2mM
+  GSM140772: 
+    treatment: AS Stress 0.2mM
+  GSM140773: 
+    treatment: AS Stress 0.2mM
+:title: 0.2 mM As(III) stressed wild-type vs (non-stressed) wild-type after 1 hour
+:platform: GPL4462

data/install_scripts/GEO/series/GSE6068.yaml

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+--- 
+:description: |-
+  Arsenic is ubiquitously present in nature and various mechanisms have
+  evolved enabling cells to evade toxicity and acquire tolerance. Herein, we
+  explored how Saccharomyces cerevisiae (budding yeast) respond to trivalent
+  arsenic (arsenite) by quantitative and kinetic transcriptome, proteome and
+  sulfur metabolite profiling. Arsenite exposure affected transcription of
+  genes encoding functions related to protein biosynthesis, arsenic
+  detoxification, oxidative stress defense, redox maintenance and
+  proteolytic activity. Importantly, enzymes involved in sulfate assimilation
+  and glutathione biosynthesis were induced at both gene and protein levels.
+  Kinetic metabolic profiling evidenced a significant increase in the pools of
+  sulfur metabolites as well as elevated glutathione levels. Moreover, the
+  flux in the sulfur assimilation pathway as well as the glutathione synthesis
+  rate strongly increased with a concomitant reduction of sulfur incorporation
+  into proteins. By combining comparative genomics and molecular analyses, we
+  pin-pointed transcription factors that mediate thecore of the
+  transcriptional response to arsenite. Taken together, our data reveals that
+  arsenite-exposed cells channel a large part of assimilated sulfur into
+  glutathione biosynthesis and we provide evidence that the transcriptional
+  regulators Yap1p and Met4p control this response in concert.
+  Keywords: stress, time course
+:arrays: 
+  GSM140775: 
+    condition: 1.0 mM As(III) stressed 15min
+  GSM140776: 
+    condition: 1.0 mM As(III) stressed 15min
+  GSM140765: 
+    condition: 1.0 mM As(III) stressed 1h
+  GSM140777: 
+    condition: 1.0 mM As(III) stressed 30min
+  GSM140778: 
+    condition: 1.0 mM As(III) stressed 30min
+  GSM140767: 
+    condition: 1.0 mM As(III) stressed 1h
+  GSM140779: 
+    condition: 1.0 mM As(III) stressed 30min
+  GSM140768: 
+    condition: 1.0 mM As(III) stressed 1h
+  GSM140780: 
+    condition: 1.0 mM As(III) stressed 18h
+  GSM140769: 
+    condition: 1.0 mM As(III) stressed 1h
+  GSM140781: 
+    condition: 1.0 mM As(III) stressed 18h
+  GSM140770: 
+    condition: 1.0 mM As(III) stressed 1h
+  GSM140782: 
+    condition: 1.0 mM As(III) stressed 18h
+  GSM140762: 
+    condition: 1.0 mM As(III) stressed 1h
+  GSM140774: 
+    condition: 1.0 mM As(III) stressed 15min
+:title: Time Course 1.0 mM As(III)
+:platform: GPL4462

data/install_scripts/GEO/series/GSE6070.yaml

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+--- 
+:title: 0.2 mM As(III) stressed yap1 deletion mutant vs 0.2 mM As(III) stressed wild-type after 1 hour
+:platform: GPL4462
+:arrays: 
+  GSM140783: 
+    condition: 0.2 mM As(III) stressed YAP1
+  GSM140784: 
+    condition: 0.2 mM As(III) stressed YAP1
+  GSM140785: 
+    condition: 0.2 mM As(III) stressed YAP1
+:description: |-
+  Arsenic is ubiquitously present in nature and various mechanisms have
+  evolved enabling cells to evade toxicity and acquire tolerance. Herein, we
+  explored how Saccharomyces cerevisiae (budding yeast) respond to trivalent
+  arsenic (arsenite) by quantitative and kinetic transcriptome, proteome and
+  sulfur metabolite profiling. Arsenite exposure affected transcription of
+  genes encoding functions related to protein biosynthesis, arsenic
+  detoxification, oxidative stress defense, redox maintenance and
+  proteolytic activity. Importantly, enzymes involved in sulfate assimilation
+  and glutathione biosynthesis were induced at both gene and protein levels.
+  Kinetic metabolic profiling evidenced a significant increase in the pools of
+  sulfur metabolites as well as elevated glutathione levels. Moreover, the
+  flux in the sulfur assimilation pathway as well as the glutathione synthesis
+  rate strongly increased with a concomitant reduction of sulfur incorporation
+  into proteins. By combining comparative genomics and molecular analyses, we
+  pin-pointed transcription factors that mediate thecore of the
+  transcriptional response to arsenite. Taken together, our data reveals that
+  arsenite-exposed cells channel a large part of assimilated sulfur into
+  glutathione biosynthesis and we provide evidence that the transcriptional
+  regulators Yap1p and Met4p control this response in concert.
+  Keywords: stress

data/install_scripts/GEO/series/GSE6071.yaml

@@ -0,0 +1,30 @@
+--- 
+:title: 1.0 mM As(III) stressed yap1 deletion mutant vs 1.0 mM As(III) stressed wild-type after 1 hour
+:platform: GPL4462
+:arrays: 
+  GSM140787: 
+    condition: 1.0 mM As(III) stressed YAP1
+  GSM140788: 
+    condition: 1.0 mM As(III) stressed YAP1
+  GSM140789: 
+    condition: 1.0 mM As(III) stressed YAP1
+:description: |-
+  volved enabling cells to evade toxicity and acquire tolerance. Herein, we
+  explored how Saccharomyces cerevisiae (budding yeast) respond to trivalent
+  arsenic (arsenite) by quantitative and kinetic transcriptome, proteome and
+  sulfur metabolite profiling. Arsenite exposure affected transcription of
+  genes encoding functions related to protein biosynthesis, arsenic
+  detoxification, oxidative stress defense, redox maintenance and
+  proteolytic activity. Importantly, enzymes involved in sulfate assimilation
+  and glutathione biosynthesis were induced at both gene and protein levels.
+  Kinetic metabolic profiling evidenced a significant increase in the pools of
+  sulfur metabolites as well as elevated glutathione levels. Moreover, the
+  flux in the sulfur assimilation pathway as well as the glutathione synthesis
+  rate strongly increased with a concomitant reduction of sulfur incorporation
+  into proteins. By combining comparative genomics and molecular analyses, we
+  pin-pointed transcription factors that mediate thecore of the
+  transcriptional response to arsenite. Taken together, our data reveals that
+  arsenite-exposed cells channel a large part of assimilated sulfur into
+  glutathione biosynthesis and we provide evidence that the transcriptional
+  regulators Yap1p and Met4p control this response in concert.
+  Keywords: stress

data/install_scripts/GEO/series/GSE6072.yaml

@@ -0,0 +1,37 @@
+--- 
+:title: 0.2 mM As(III) stressed met4 deletion mutant vs 0.2 mM As(III) stressed wild-type after 1 hour
+:platform: GPL4462
+:arrays: 
+  GSM140790: 
+    condition: 0.2 mM As(III) stressed MET4
+  GSM140791: 
+    condition: 0.2 mM As(III) stressed MET4
+  GSM140793: 
+    condition: 0.2 mM As(III) stressed MET4
+  GSM140794: 
+    condition: 0.2 mM As(III) stressed MET4
+  GSM140795: 
+    condition: 0.2 mM As(III) stressed MET4
+  GSM140796: 
+    condition: 0.2 mM As(III) stressed MET4
+:description: |-
+  Arsenic is ubiquitously present in nature and various mechanisms have
+  evolved enabling cells to evade toxicity and acquire tolerance. Herein, we
+  explored how Saccharomyces cerevisiae (budding yeast) respond to trivalent
+  arsenic (arsenite) by quantitative and kinetic transcriptome, proteome and
+  sulfur metabolite profiling. Arsenite exposure affected transcription of
+  genes encoding functions related to protein biosynthesis, arsenic
+  detoxification, oxidative stress defense, redox maintenance and
+  proteolytic activity. Importantly, enzymes involved in sulfate assimilation
+  and glutathione biosynthesis were induced at both gene and protein levels.
+  Kinetic metabolic profiling evidenced a significant increase in the pools of
+  sulfur metabolites as well as elevated glutathione levels. Moreover, the
+  flux in the sulfur assimilation pathway as well as the glutathione synthesis
+  rate strongly increased with a concomitant reduction of sulfur incorporation
+  into proteins. By combining comparative genomics and molecular analyses, we
+  pin-pointed transcription factors that mediate thecore of the
+  transcriptional response to arsenite. Taken together, our data reveals that
+  arsenite-exposed cells channel a large part of assimilated sulfur into
+  glutathione biosynthesis and we provide evidence that the transcriptional
+  regulators Yap1p and Met4p control this response in concert.
+  Keywords: stress

data/install_scripts/GEO/series/GSE6101.yaml

@@ -0,0 +1,26 @@
+--- 
+:title: 300 ppm Citrinin treatment for 2 h (GPL4399)
+:platform: GPL4399
+:arrays: 
+  GSM141466: 
+    condition: citrinin treated for 2 h
+  GSM141455: 
+    condition: citrinin treated for 2 h
+  GSM141467: 
+    condition: citrinin treated for 2 h
+  GSM141456: 
+    condition: citrinin treated for 2 h
+  GSM141468: 
+    condition: citrinin treated for 2 h
+  GSM141457: 
+    condition: citrinin treated for 2 h
+  GSM141469: 
+    condition: citrinin treated for 2 h
+  GSM141470: 
+    condition: citrinin treated for 2 h
+  GSM141471: 
+    condition: citrinin treated for 2 h
+:description: |-
+  The availability of yeast DNA microarrays provides the possibility of monitoring gene expression levels as a function to toxin exposure, and consequently as a means of determining mechanisms of toxicity. This system possesses the benefit of the essential volume of yeast cultures, the high reproducibility of expression profiles, and the massive functional information. Because small amount of biological sample cultures are required for this analysis, toxicity test for rare chemical such as mycotoxins which is a natural compound and difficult to be artificially synthesized. Citrinin [518-75-2], 4,6-dihydro-8-hydroxy-3,4,5-trimethyl-6-oxo-3H-2-benzopyran-7-crboxylic acid, is the one of the popular mycotoxicin produced by Penicillium and Aspergillus family possibly spread all over the world. This natural chemicals is one of the well characterized mycotoxin but the information especially mechanism of toxic action is limited.
+  We used two types of microarrays, one have ORF (Open reading Frame) fragment on the surface of the glass as probes and the other have probes as oligonucleotide probes on the microarray with 3 dimensions. We compared data properties and studied the toxicity of citrinin to yeast cells.
+  Keywords: stress response

data/install_scripts/GEO/series/GSE6111.yaml

@@ -0,0 +1,20 @@
+--- 
+:title: 300 ppm Citrinin treatment for 2 h (GPL1945)
+:platform: GPL1945
+:arrays: 
+  GSM142075: 
+    condition: citrinin treated for 2 h
+  GSM142076: 
+    condition: citrinin treated for 2 h
+  GSM142077: 
+    condition: citrinin treated for 2 h
+  GSM142078: 
+    condition: citrinin treated for 2 h
+  GSM141513: 
+    condition: citrinin treated for 2 h
+  GSM142080: 
+    condition: citrinin treated for 2 h
+:description: |-
+  The availability of yeast DNA microarrays provides the possibility of monitoring gene expression levels as a function to toxin exposure, and consequently as a means of determining mechanisms of toxicity. This system possesses the benefit of the essential volume of yeast cultures, the high reproducibility of expression profiles, and the massive functional information. Because small amount of biological sample cultures are required for this analysis, toxicity test for rare chemical such as mycotoxins which is a natural compound and difficult to be artificially synthesized. Citrinin [518-75-2], 4,6-dihydro-8-hydroxy-3,4,5-trimethyl-6-oxo-3H-2-benzopyran-7-crboxylic acid, is the one of the popular mycotoxicin produced by Penicillium and Aspergillus family possibly spread all over the world. This natural chemicals is one of the well characterized mycotoxin but the information especially mechanism of toxic action is limited.
+  We used two types of microarrays, one have ORF (Open reading Frame) fragment on the surface of the glass as probes and the other have probes as oligonucleotide probes on the microarray with 3 dimensions. We compared data properties and studied the toxicity of citrinin to yeast cells.
+  Keywords: stress response

data/install_scripts/GEO/series/GSE6190.yaml

@@ -0,0 +1,30 @@
+--- 
+:arrays: 
+  GSM143076: 
+    condition: 12C Chemostat anaerobic C-lim 0.03h
+  GSM143088: 
+    condition: 30C anaerobic N-lim chemostat 0.03h
+  GSM143077: 
+    condition: 30C Anaerobic C-lim chemostat 0.03h
+  GSM143089: 
+    condition: 30C anaerobic N-lim chemostat 0.03h
+  GSM143079: 
+    condition: 30C Anaerobic C-lim chemostat 0.03h
+  GSM143081: 
+    condition: 30C Anaerobic C-lim chemostat 0.03h
+  GSM143082: 
+    condition: 12C anaerobic N-lim chemostat 0.03h
+  GSM143071: 
+    condition: 12C Chemostat anaerobic C-lim 0.03h
+  GSM143083: 
+    condition: 12C anaerobic N-lim chemostat 0.03h
+  GSM143084: 
+    condition: 12C anaerobic N-lim chemostat 0.03h
+  GSM143073: 
+    condition: 12C Chemostat anaerobic C-lim 0.03h
+  GSM143085: 
+    condition: 30C anaerobic N-lim chemostat 0.03h
+:description: "The global transcriptional response of Saccharomyces cerevisiae was investigated in low temperature chemostat cultures grown in carbon or nitrogen limitation. During steady state chemostats, the growth rates and in vivo fluxes were kept constant however the growth-limiting nutrient was significantly higher at 12oC than at 30oC and had significant effects on transcriptional responses. Growth at 12oC resulted in a rearrangement of transporters for the limiting nutrient, where hexose transporters (HXTs) and ammonium permeases (MEPs) were differentially expressed in cultures grown at 30oC in carbon and nitrogen limitations, respectively. In addition, we found repression of genes encoding proteins in reserve carbohydrates metabolism and metabolism of alternative carbon or nitrogen sources other than glucose or ammonia. However, there were also similar responses when the transcriptional response was evaluated regardless of the growth-limiting nutrient. In particular, induction of ribosome biogenesis genes emphasizes the significance of transcription and translational adaptation at low temperature. In contrast, genes encoding proteins during stress response were downregulated. This down-regulation of stress elements better known as environmental stress response (ESR) is in contradiction with previous low temperature transcriptome analyses. During continuous steady state low temperature cultivation, ESR no longer plays an integral role in S. cerevisiae\xE2\x80\x99s response to temperature change. Similarly, trehalose accumulation, consistent with its gene expression, was not indispensable for growth at 12oC. This response, however, does not exclude that ESR may be required for transition phase in low temperature growth when cells are transferred from one temperature to another.\n\
+  Keywords: chemostat temperature 12 degree celsuis 30 degree celsius"
+:title: Temperature-dependent transcriptional response under anaerobic C and N limitations in Yeast
+:platform: GPL90

data/install_scripts/GEO/series/GSE6277.yaml

@@ -0,0 +1,19 @@
+--- 
+:arrays: 
+  GSM144220: 
+    condition: CEN.PK113-7D/pYX212 chemostat at Dcrit
+  GSM144221: 
+    condition: CEN.PK113-7D/pYX212 chemostat at Dcrit
+  GSM144222: 
+    condition: CEN.PK113-7D/pYX212-NOX chemostat at Dcrit
+  GSM144223: 
+    condition: CEN.PK113-7D/pYX212-NOX chemostat at Dcrit
+  GSM144224: 
+    condition: CEN.PK113-7D/pYX212-AOX chemostat at Dcrit
+  GSM144225: 
+    condition: CEN.PK113-7D/pYX212-AOX chemostat at Dcrit
+:description: |-
+  When the yeast Saccharomyces cerevisiae is subjected to increasing glycolytic fluxes under aerobic conditions, there is a threshold value of the glucose uptake rate at which the metabolism shifts from being purely respiratory to mixed respiratory and fermentative. This shift is characterized by ethanol production, a phenomenon known as the Crabtree effect due to its analogy with lactate overflow in cancer cells. It is well known that at high glycolytic fluxes there is glucose repression of respiratory pathways resulting in a decrease in the respiratory capacity. Despite many years of detailed studies on this subject, it is not known whether the onset of the Crabtree effect (or overflow metabolism) is due to a limited respiratory capacity or caused by glucose-mediated repression of respiration. We addressed this issue by increasing respiration in S. cerevisiae by introducing a heterologous alternative oxidase, and observed reduced aerobic ethanol formation. In contrast, increasing non-respiratory NADH oxidation by overexpression of a water-forming NADH oxidase reduced aerobic glycerol formation. The metabolic response to elevated alternative oxidase occurred predominantly in the mitochondria, while NADH oxidase affected genes that catalyze cytosolic reactions. Moreover, NADH oxidase restored the deficiency of cytosolic NADH dehydrogenases in S. cerevisiae. These results indicate that NADH oxidase localizes in the cytosol, while alternative oxidase is directed to the mitochondria. The onset of aerobic ethanol formation is demonstrated to be a consequence of an imbalance in mitochondrial redox balancing. In addition to answering fundamental physiological questions, our findings are relevant for all biomass derived applications of S. cerevisiae.
+  Keywords: Genetic Modification
+:title: Increasing NADH oxidation reduces overflow metabolism in Saccharomyces cerevisiae
+:platform: GPL2529

data/install_scripts/GEO/series/GSE6331.yaml

@@ -0,0 +1,51 @@
+--- 
+:arrays: 
+  GSM146328: 
+    mutant: H39h
+  GSM146317: 
+    mutant: wt0h
+  GSM146306: 
+    mutant: H3
+  GSM146329: 
+    mutant: H39h
+  GSM146318: 
+    mutant: H30h
+  GSM146320: 
+    mutant: wt6h
+  GSM146319: 
+    mutant: H30h
+  GSM146321: 
+    mutant: wt6h
+  GSM146322: 
+    mutant: wt6h
+  GSM146323: 
+    mutant: H36h
+  GSM146301: 
+    mutant: wt
+  GSM146324: 
+    mutant: H36h
+  GSM146302: 
+    mutant: wt
+  GSM146325: 
+    mutant: H36h
+  GSM146303: 
+    mutant: wt
+  GSM146326: 
+    mutant: wt9h
+  GSM146304: 
+    mutant: H3
+  GSM146327: 
+    mutant: wt9h
+  GSM146316: 
+    mutant: wt0h
+  GSM146305: 
+    mutant: H3
+:description: |-
+  This SuperSeries is composed of the following subset Series:
+  GSE6319: Histone H3 K4,79R
+  GSE6326: Histone H3 K4,36,79R 0 hr
+  GSE6327: Histone H3 K4,36,79R 6 hr
+  GSE6328: Histone H3 K4,36,79R 9 hr
+  Keywords: SuperSeries
+:title: Histone H3,K4,79R and H3,K4,36,79R (at 0, 6, and 9 hours) Mutations
+:platform: GPL90

data/install_scripts/GEO/series/GSE6346.yaml

@@ -0,0 +1,49 @@
+--- 
+:arrays: 
+  GSM146692: 
+    treatment: none
+    stimulation: Polyl C
+  GSM146693: 
+    treatment: none
+    stimulation: Polyl C
+  GSM146682: 
+    treatment: none
+    stimulation: LPS
+  GSM146694: 
+    treatment: GW3965
+    stimulation: Polyl C
+  GSM146695: 
+    treatment: GW3965
+    stimulation: Polyl C
+  GSM146684: 
+    treatment: none
+    stimulation: LPS
+  GSM146696: 
+    treatment: GW7845
+    stimulation: Polyl C
+  GSM146697: 
+    treatment: GW7845
+    stimulation: Polyl C
+  GSM146686: 
+    treatment: GW3965
+    stimulation: LPS
+  GSM146687: 
+    treatment: GW3965
+    stimulation: LPS
+  GSM146688: 
+    treatment: GW7845
+    stimulation: LPS
+  GSM146690: 
+    treatment: none
+    stimulation: none
+  GSM146689: 
+    treatment: GW7845
+    stimulation: LPS
+  GSM146691: 
+    treatment: none
+    stimulation: none
+:description: "Transrepression mediated by PPARg and LXRs agonists (GW7845 and GW3965) in primary macrophages stimulated with LPS or poly I:C. Thioglycollate-elicited macrophages were isolated from mice by peritoneal lavage 3 days following peritoneal injection of 2.5 ml 3% thioglycollate (DIFCO). Cells were plated in RPMI medium 1640 and 10% fetal bovine serum, washed after 5 h the medium was removed and cells were fed with fresh medium containing 0.5% fetal bovine serum. Cells were treated with LPS (Sigma) a concentration of 100 ng/ml or poly I:C (100 ng/ml) in the absence or presence of receptor-specific agonists for PPAR\xCE\xB3 (GW7845) or LXR (GW3965) at 1 \xC2\xB5M concentrations. Results suggest signal-dependent nuclear receptors transrepression pathways.\n\
+  Reference: Molecular Determinants of Crosstalk between Nuclear Receptors and Toll-like Receptors Mediating Counter-regulation of Inflammatory Responses. Sumito Ogawa, Jean Lozach, Chris Benner, Gabriel Pascual, Rajendra K. Tangirala, Stefan Westin, Alexander Hoffmann, Shankar Subramaniam, Michael David, Michael G. Rosenfeld, and Christopher K. Glass. Cell, Vol 122, 707-721, 9 September 2005.\n\
+  Keywords: PPARg and LXRs agonists"
+:title: Differential repression of TLR responses by PPARg and LXRs
+:platform: GPL1310