@pikaa-ai/pikaa 0.3.22 → 0.3.24
This diff represents the content of publicly available package versions that have been released to one of the supported registries. The information contained in this diff is provided for informational purposes only and reflects changes between package versions as they appear in their respective public registries.
- package/assets/brand/orbit-logo-option4-whale.jpg +0 -0
- package/assets/brand/orbit-logo.jpg +0 -0
- package/assets/brand/orbit-logo.png +0 -0
- package/assets/brand/orbit-logo.svg +3 -0
- package/dist/cli.js +448 -181
- package/dist/index.js +22 -2
- package/package.json +1 -2
- package/skills/adaptyv/SKILL.md +0 -240
- package/skills/aeon/SKILL.md +0 -402
- package/skills/analytical-method-validation/SKILL.md +0 -299
- package/skills/anndata/SKILL.md +0 -431
- package/skills/arbor/SKILL.md +0 -152
- package/skills/arboreto/SKILL.md +0 -267
- package/skills/astropy/SKILL.md +0 -353
- package/skills/autoskill/SKILL.md +0 -233
- package/skills/benchling-integration/SKILL.md +0 -229
- package/skills/bgpt-paper-search/SKILL.md +0 -75
- package/skills/bids/SKILL.md +0 -237
- package/skills/biopython/SKILL.md +0 -472
- package/skills/bioservices/SKILL.md +0 -399
- package/skills/bulk-rnaseq/SKILL.md +0 -198
- package/skills/cellxgene-census/SKILL.md +0 -283
- package/skills/cirq/SKILL.md +0 -370
- package/skills/citation-management/SKILL.md +0 -329
- package/skills/clinical-decision-support/SKILL.md +0 -238
- package/skills/clinical-decision-support/references/README.md +0 -62
- package/skills/clinical-reports/SKILL.md +0 -248
- package/skills/clinical-reports/references/README.md +0 -34
- package/skills/cobrapy/SKILL.md +0 -496
- package/skills/consciousness-council/SKILL.md +0 -151
- package/skills/dask/SKILL.md +0 -482
- package/skills/database-lookup/SKILL.md +0 -386
- package/skills/datamol/SKILL.md +0 -200
- package/skills/deepchem/SKILL.md +0 -244
- package/skills/deepspot-m/SKILL.md +0 -175
- package/skills/deeptools/SKILL.md +0 -412
- package/skills/depmap/SKILL.md +0 -301
- package/skills/dhdna-profiler/SKILL.md +0 -184
- package/skills/diffdock/SKILL.md +0 -488
- package/skills/dnanexus-integration/SKILL.md +0 -325
- package/skills/docx/SKILL.md +0 -99
- package/skills/esm/SKILL.md +0 -334
- package/skills/etetoolkit/SKILL.md +0 -327
- package/skills/exa-search/SKILL.md +0 -102
- package/skills/executing-plans/SKILL.md +0 -14
- package/skills/experimental-design/SKILL.md +0 -234
- package/skills/exploratory-data-analysis/SKILL.md +0 -280
- package/skills/flowio/SKILL.md +0 -310
- package/skills/fluidsim/SKILL.md +0 -279
- package/skills/frontend-design/SKILL.md +0 -100
- package/skills/generate-image/SKILL.md +0 -304
- package/skills/geniml/SKILL.md +0 -310
- package/skills/genomic-coordinates/SKILL.md +0 -189
- package/skills/genomic-intelligence/SKILL.md +0 -243
- package/skills/geomaster/README.md +0 -105
- package/skills/geomaster/SKILL.md +0 -366
- package/skills/geopandas/SKILL.md +0 -250
- package/skills/get-available-resources/SKILL.md +0 -260
- package/skills/gget/SKILL.md +0 -153
- package/skills/ginkgo-cloud-lab/SKILL.md +0 -106
- package/skills/glycoengineering/SKILL.md +0 -339
- package/skills/gtars/SKILL.md +0 -282
- package/skills/guardian-rails/SKILL.md +0 -54
- package/skills/histolab/SKILL.md +0 -243
- package/skills/hugging-science/SKILL.md +0 -132
- package/skills/hypogenic/SKILL.md +0 -290
- package/skills/hypothesis-generation/SKILL.md +0 -264
- package/skills/imaging-data-commons/SKILL.md +0 -496
- package/skills/infographics/SKILL.md +0 -315
- package/skills/iso-standards-readiness/SKILL.md +0 -352
- package/skills/lab-hardware-cad/SKILL.md +0 -372
- package/skills/labarchive-integration/SKILL.md +0 -216
- package/skills/lamindb/SKILL.md +0 -408
- package/skills/latchbio-integration/SKILL.md +0 -227
- package/skills/latex-posters/SKILL.md +0 -369
- package/skills/latex-posters/references/README.md +0 -439
- package/skills/liteparse/SKILL.md +0 -295
- package/skills/literature-review/SKILL.md +0 -263
- package/skills/markdown-mermaid-writing/SKILL.md +0 -322
- package/skills/market-research-reports/SKILL.md +0 -337
- package/skills/markitdown/SKILL.md +0 -264
- package/skills/matchms/SKILL.md +0 -276
- package/skills/matlab/SKILL.md +0 -274
- package/skills/matplotlib/SKILL.md +0 -378
- package/skills/medchem/SKILL.md +0 -321
- package/skills/modal/SKILL.md +0 -468
- package/skills/molecular-dynamics/SKILL.md +0 -458
- package/skills/molfeat/SKILL.md +0 -348
- package/skills/ncats-arax/SKILL.md +0 -178
- package/skills/networkx/SKILL.md +0 -440
- package/skills/neurokit2/SKILL.md +0 -323
- package/skills/neuropixels-analysis/SKILL.md +0 -412
- package/skills/nextflow/SKILL.md +0 -195
- package/skills/omero-integration/SKILL.md +0 -222
- package/skills/onekgpd/SKILL.md +0 -371
- package/skills/ontology-term-resolution/SKILL.md +0 -147
- package/skills/open-notebook/SKILL.md +0 -297
- package/skills/openpiv/SKILL.md +0 -469
- package/skills/opentrons-integration/SKILL.md +0 -322
- package/skills/optimize-for-gpu/SKILL.md +0 -176
- package/skills/owasp-top10/SKILL.md +0 -48
- package/skills/pacsomatic/LICENSE +0 -21
- package/skills/pacsomatic/SKILL.md +0 -150
- package/skills/paper-lookup/SKILL.md +0 -263
- package/skills/paperclip/SKILL.md +0 -413
- package/skills/paperzilla/SKILL.md +0 -159
- package/skills/parallel-web/SKILL.md +0 -128
- package/skills/pathml/SKILL.md +0 -222
- package/skills/pathogen-variant-surveillance/SKILL.md +0 -208
- package/skills/pathway-enrichment/SKILL.md +0 -194
- package/skills/pdf/SKILL.md +0 -322
- package/skills/peer-review/SKILL.md +0 -288
- package/skills/penetration-testing/SKILL.md +0 -31
- package/skills/pennylane/SKILL.md +0 -240
- package/skills/phylogenetics/SKILL.md +0 -409
- package/skills/pi-agent/SKILL.md +0 -83
- package/skills/pkpd-modeling/SKILL.md +0 -381
- package/skills/polars/SKILL.md +0 -393
- package/skills/polars-bio/SKILL.md +0 -379
- package/skills/ponytail/SKILL.md +0 -31
- package/skills/ponytail-audit/SKILL.md +0 -18
- package/skills/pptx/SKILL.md +0 -246
- package/skills/pptx-posters/SKILL.md +0 -258
- package/skills/primekg/SKILL.md +0 -99
- package/skills/protocolsio-integration/SKILL.md +0 -236
- package/skills/pufferlib/SKILL.md +0 -328
- package/skills/pydeseq2/SKILL.md +0 -369
- package/skills/pydicom/SKILL.md +0 -381
- package/skills/pyhealth/SKILL.md +0 -124
- package/skills/pylabrobot/SKILL.md +0 -216
- package/skills/pymatgen/SKILL.md +0 -404
- package/skills/pymc/SKILL.md +0 -310
- package/skills/pymoo/SKILL.md +0 -276
- package/skills/pyopenms/SKILL.md +0 -179
- package/skills/pysam/SKILL.md +0 -330
- package/skills/pytdc/SKILL.md +0 -297
- package/skills/pytorch-lightning/SKILL.md +0 -191
- package/skills/pyzotero/SKILL.md +0 -137
- package/skills/qiskit/SKILL.md +0 -259
- package/skills/qutip/SKILL.md +0 -317
- package/skills/rdkit/SKILL.md +0 -94
- package/skills/relsa-severity-assessment/SKILL.md +0 -354
- package/skills/research-grants/SKILL.md +0 -296
- package/skills/research-grants/references/README.md +0 -287
- package/skills/research-lookup/README.md +0 -106
- package/skills/research-lookup/SKILL.md +0 -338
- package/skills/rowan/SKILL.md +0 -398
- package/skills/scanpy/SKILL.md +0 -303
- package/skills/scholar-evaluation/SKILL.md +0 -296
- package/skills/scientific-brainstorming/SKILL.md +0 -282
- package/skills/scientific-critical-thinking/SKILL.md +0 -180
- package/skills/scientific-schematics/SKILL.md +0 -370
- package/skills/scientific-slides/SKILL.md +0 -379
- package/skills/scientific-visualization/SKILL.md +0 -285
- package/skills/scientific-writing/SKILL.md +0 -356
- package/skills/scikit-bio/SKILL.md +0 -470
- package/skills/scikit-learn/SKILL.md +0 -324
- package/skills/scikit-survival/SKILL.md +0 -313
- package/skills/scvelo/SKILL.md +0 -328
- package/skills/scvi-tools/SKILL.md +0 -201
- package/skills/seaborn/SKILL.md +0 -254
- package/skills/security-auditor/SKILL.md +0 -37
- package/skills/shap/SKILL.md +0 -282
- package/skills/simpy/SKILL.md +0 -283
- package/skills/stable-baselines3/SKILL.md +0 -325
- package/skills/statistical-analysis/SKILL.md +0 -446
- package/skills/statistical-power/SKILL.md +0 -200
- package/skills/statsmodels/SKILL.md +0 -238
- package/skills/sympy/SKILL.md +0 -354
- package/skills/systematic-debugging/SKILL.md +0 -35
- package/skills/tamarind/SKILL.md +0 -285
- package/skills/tdd/SKILL.md +0 -26
- package/skills/tiledbvcf/SKILL.md +0 -456
- package/skills/timesfm-forecasting/SKILL.md +0 -408
- package/skills/timesfm-forecasting/examples/global-temperature/README.md +0 -178
- package/skills/torch-geometric/SKILL.md +0 -458
- package/skills/torchdrug/SKILL.md +0 -241
- package/skills/transformers/SKILL.md +0 -195
- package/skills/treatment-plans/SKILL.md +0 -174
- package/skills/treatment-plans/references/README.md +0 -19
- package/skills/umap-learn/SKILL.md +0 -488
- package/skills/uncertainty-and-units/SKILL.md +0 -384
- package/skills/usfiscaldata/SKILL.md +0 -171
- package/skills/vaex/SKILL.md +0 -204
- package/skills/venue-templates/SKILL.md +0 -269
- package/skills/verification-before-completion/SKILL.md +0 -22
- package/skills/waypoint-bio/SKILL.md +0 -273
- package/skills/what-if-oracle/SKILL.md +0 -184
- package/skills/writing-plans/SKILL.md +0 -15
- package/skills/xlsx/SKILL.md +0 -110
- package/skills/zarr-python/SKILL.md +0 -241
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name: parallel-web
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description: "Use Parallel CLI for web search, URL extraction, deep research, structured data enrichment, entity discovery, and recurring web monitoring. Best for requests that explicitly need current web evidence, academic-source discovery, repeated entity lookups, exhaustive reports, or ongoing change tracking."
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license: MIT
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compatibility: Requires parallel-cli and internet access.
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metadata:
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version: "1.2"
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author: K-Dense, Inc.
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openclaw:
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primaryEnv: PARALLEL_API_KEY
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envVars:
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required: true
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description: Parallel API key.
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---
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# Parallel Web Toolkit
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A unified skill for Parallel's web-intelligence workflows. For scientific topics, prefer primary literature and authoritative institutional sources.
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## Routing — pick the right capability
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Read the user's request and then open the corresponding reference file before running a command.
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| User wants to... | Capability | Where |
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| Look something up, research a topic, find current info | **Web Search** | `references/web-search.md` |
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| Fetch content from a specific URL (webpage, article, PDF) | **Web Extract** | `references/web-extract.md` |
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| Add web-sourced fields to a list of companies/people/products | **Data Enrichment** | `references/data-enrichment.md` |
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| Get an exhaustive, multi-source report (user says "deep research", "exhaustive", "comprehensive") | **Deep Research** | `references/deep-research.md` |
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| Discover a set of entities matching natural-language criteria | **FindAll** | `references/findall.md` |
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| Track web changes on a recurring schedule | **Monitor** | `references/monitor.md` |
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| Check or retrieve an asynchronous result | **Status and polling** | Below and the capability reference |
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### Decision guide
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- **Web Extract** is for a known public URL, including PDFs and JavaScript-rendered pages.
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- **Data Enrichment** applies the same requested fields to user-supplied rows. Do not loop over Web Search for this.
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- **FindAll** discovers the entities themselves. Use enrichment when the entities are already supplied.
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- **Deep Research** is only for explicitly exhaustive or comprehensive requests because it is slower and more expensive.
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- Institutional and government sources (NIH, WHO, NASA, NIST) over commercial sites
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name: pathml
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description: "Use PathML for local, research-only computational pathology workflows: load and tile slides, build preprocessing and QC pipelines, manage h5path data, quantify multiplex images, construct spatial graphs, and plan bounded model inference."
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license: MIT
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compatibility: PathML 3.0.5 is the latest PyPI release and targets Python 3.10-3.12; installation needs uv plus platform libraries for OpenSlide, BLAS/LAPACK, and Java/Bio-Formats. Bundled Python 3.10+ CLIs are local, bounded, dependency-free, and network-free.
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metadata:
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version: "1.1"
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skill-author: K-Dense Inc.
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---
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# PathML
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## Scope and safety boundary
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Use PathML for **local computational pathology research**. It is beta research
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software, not a validated medical device, diagnostic system, clinical decision
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support tool, or substitute for a pathologist. Do not use outputs to diagnose,
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grade, stage, or treat a patient.
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Pathology files may contain faces, labels, accession numbers, patient identifiers,
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DICOM tags, filenames, or linked clinical data. Before processing:
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1. Confirm authorization, consent/waiver, data-use terms, and institutional policy.
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2. De-identify pixels and metadata; keep the re-identification key outside the
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analysis workspace.
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3. Use pseudonymous `patient_id`, `slide_id`, and `specimen_id` values. Do not put
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direct identifiers in filenames, logs, `.h5path` labels, model cards, or reports.
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4. Keep inputs, intermediates, and outputs on approved local encrypted storage.
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5. Split by patient (then slide) before tiling or fitting any preprocessing step.
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## Version baseline, verified 2026-07-23
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- **Installable stable release:** PyPI `pathml==3.0.5`, published 2026-03-24.
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- The v3.0.5 release notes state Python **3.10-3.12** and sunset 3.9.
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PyPI does not declare `Requires-Python` and still has a stale 3.8 classifier, so
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use the release statement and test the exact environment.
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- GitHub releases v3.0.6 (2026-04-14) and v3.0.7 (2026-07-09) exist, but PyPI has
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no artifacts for them as of this review. v3.0.7 updates Torch/TorchVision/
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torch-geometric and ONNX export code. Do not mix those source dependencies with
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the 3.0.5 wheel.
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- ReadTheDocs `/latest` identifies itself as 3.0.5. Examples here were checked
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against the v3.0.5 tag and PyPI wheel metadata, not unversioned snippets.
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- This skill is MIT-licensed. PathML itself is GPL-2.0 with upstream commercial
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licensing options; review upstream terms before redistribution.
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## Reproducible installation
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Use Python 3.11 unless the project has tested another supported interpreter:
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```bash
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uv venv --python 3.11
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source .venv/bin/activate
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uv pip install "pathml==3.0.5"
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python -c "import importlib.metadata as m; print(m.version('pathml'))"
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```
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PathML 3.0.5 declares no package extras: do **not** use `pathml[all]`. Its base
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distribution pins a large scientific/ML stack, including Torch 2.8.0, ONNX 1.17.0,
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ONNX Runtime 1.17.x, OpenSlide Python 1.3.1, python-bioformats 4.1.0, and
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python-javabridge 4.0.4.
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Install native prerequisites before the uv command:
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```bash
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# Debian/Ubuntu
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sudo apt-get install openslide-tools gcc g++ libblas-dev liblapack-dev openjdk-17-jdk
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# macOS
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brew install openslide openjdk@17
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# Windows OpenSlide option documented upstream
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vcpkg install openslide
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```
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|
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|
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Java/Bio-Formats is needed for the broad multidimensional format backend.
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OpenSlide handles common brightfield WSI formats more efficiently. CUDA is
|
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optional and must match the pinned PyTorch build; follow PyTorch's platform
|
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selector rather than guessing a CUDA wheel. See `references/image_loading.md`.
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## Stable minimal workflow
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PathML 3.0.5 uses slide convenience classes and `SlideData.run()`. It does not
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provide `SlideData.from_slide()`, and `Pipeline` does not have `run()`:
|
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|
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|
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|
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```python
|
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from pathml.core import HESlide
|
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|
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from pathml.preprocessing import BoxBlur, Pipeline, TissueDetectionHE
|
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|
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|
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slide = HESlide("data/pseudonymous_slide.svs", backend="openslide")
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pipeline = Pipeline(
|
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[
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BoxBlur(kernel_size=5),
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TissueDetectionHE(mask_name="tissue", min_region_size=5000),
|
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]
|
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)
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slide.run(
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pipeline,
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distributed=False,
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tile_size=512,
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tile_stride=512,
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level=0,
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tile_pad=False,
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)
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slide.write("derived/pseudonymous_slide.h5path")
|
|
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```
|
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|
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Start with a bounded manual sample before a full run:
|
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|
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|
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```python
|
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|
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from itertools import islice
|
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|
-
|
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for tile in islice(slide.generate_tiles(shape=512, stride=512, level=0), 8):
|
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pipeline.apply(tile)
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|
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assert tile.masks["tissue"].shape[:2] == tile.image.shape[:2]
|
|
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-
```
|
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|
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Tiles use `(i, j)` = `(row, column)` coordinates at the selected pyramid level.
|
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For OpenSlide, PathML maps them to level-0 coordinates internally. Record the
|
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level and downsample; convert to `(x, y)` or micrometres explicitly downstream.
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|
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|
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## Research workflow
|
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|
|
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|
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1. **Inventory locally.** Validate the manifest, reject URLs/symlinks, inspect only
|
|
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|
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allowlisted technical metadata, and remove identifiers.
|
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|
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2. **Freeze splits.** Assign every patient and all their slides to one split before
|
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|
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generating overlapping tiles, graphs, normalization references, or features.
|
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|
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3. **Plan bounds.** Estimate tile count, RAM, output size, and pipeline stages.
|
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|
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4. **Pilot preprocessing.** Inspect tissue masks, whitespace/artifact labels,
|
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|
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stain behavior, edge padding, and empty-mask cases on representative training
|
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|
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slides. Do not tune from test slides.
|
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|
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5. **Run and preserve coordinates.** Keep tile level, `(i, j)`, downsample, MPP,
|
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|
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mask names, QC decisions, and failed/skipped tiles.
|
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|
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6. **Build spatial data deliberately.** Validate channel order, physical units,
|
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|
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instance labels, node-feature alignment, graph edges, and cell-to-tissue
|
|
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|
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assignments.
|
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|
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7. **Infer in bounded batches.** Verify model provenance and checksum without
|
|
138
|
-
loading unknown pickle checkpoints. Keep predictions linked to slide/tile
|
|
139
|
-
coordinates and stitch overlaps with a documented rule.
|
|
140
|
-
8. **Report provenance and limits.** Include package lock, source hashes, scanner,
|
|
141
|
-
stain, parameters, seeds, split manifest, model card, exclusions, and QC.
|
|
142
|
-
|
|
143
|
-
## No-network default and explicit consent gate
|
|
144
|
-
|
|
145
|
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Do not instantiate download-capable classes or set dataset `download=True` unless
|
|
146
|
-
the user explicitly opts in after receiving the endpoint and disclosure:
|
|
147
|
-
|
|
148
|
-
- `SegmentMIFRemote` downloads an ONNX file from
|
|
149
|
-
`https://huggingface.co/pathml/test/resolve/main/mesmer.onnx` at construction,
|
|
150
|
-
then runs inference locally. Stable source does **not** upload image pixels.
|
|
151
|
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The request still discloses network metadata such as IP address and headers and
|
|
152
|
-
creates `temp.onnx`; there is no built-in checksum or offline flag.
|
|
153
|
-
- Deprecated `SegmentMIF` imports local DeepCell Mesmer, but DeepCell model
|
|
154
|
-
initialization may need separately provisioned weights. It is not a PathML
|
|
155
|
-
extra and is not the preferred stable API.
|
|
156
|
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- `RemoteTestHoverNet` downloads a model from Hugging Face.
|
|
157
|
-
- `PanNukeDataModule(download=True)` contacts Warwick; `DeepFocusDataModule`
|
|
158
|
-
contacts Zenodo. Both default to `download=False`.
|
|
159
|
-
|
|
160
|
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Before any future hosted prediction call, state the exact destination, pixel
|
|
161
|
-
channels/regions, metadata, identifiers, retention, legal basis, and safeguards;
|
|
162
|
-
obtain explicit consent; and never send PHI by default. Prefer reviewed,
|
|
163
|
-
checksummed local model artifacts and local inference.
|
|
164
|
-
|
|
165
|
-
## Model-code security
|
|
166
|
-
|
|
167
|
-
- PyTorch `model.eval()` means **evaluation mode** for modules; it is not Python's
|
|
168
|
-
dangerous built-in evaluator. Never use Python dynamic evaluation or execution.
|
|
169
|
-
- Do not name local files `pathml.py`, `torch.py`, `onnx.py`, or after standard
|
|
170
|
-
libraries; shadow modules can silently change imports.
|
|
171
|
-
- PathML's `EntityDataset` loads `.pt` objects with `weights_only=False`. Never
|
|
172
|
-
open an untrusted graph/checkpoint. Treat pickle-based pipelines and `.pt` files
|
|
173
|
-
as executable code.
|
|
174
|
-
- ONNX is safer than pickle but not inherently trusted. Verify source, SHA-256,
|
|
175
|
-
expected input/output schema, file size, and runtime limits; use isolation for
|
|
176
|
-
third-party models.
|
|
177
|
-
|
|
178
|
-
## Bundled local CLIs
|
|
179
|
-
|
|
180
|
-
All helpers reject URLs and symlinks, cap inputs/work, use strict JSON, avoid
|
|
181
|
-
network access, and require no PathML import for `--help`:
|
|
182
|
-
|
|
183
|
-
```bash
|
|
184
|
-
python scripts/slide_manifest.py validate --manifest manifest.csv --root .
|
|
185
|
-
python scripts/slide_manifest.py inspect --slide data/example.svs --root .
|
|
186
|
-
python scripts/plan_pipeline.py --width 100000 --height 80000 --tile-size 512 --stride 512
|
|
187
|
-
python scripts/image_qc.py synthetic --width 256 --height 256
|
|
188
|
-
python scripts/validate_spatial_schema.py graph --input graph.json --root .
|
|
189
|
-
python scripts/validate_spatial_schema.py multiplex --input cells.csv --root .
|
|
190
|
-
python scripts/plan_inference.py --tile-count 4000 --batch-size 16 --height 256 --width 256
|
|
191
|
-
```
|
|
192
|
-
|
|
193
|
-
The inference planner reads numbers or a bounded JSON model card only; it never
|
|
194
|
-
imports a model framework or opens a checkpoint.
|
|
195
|
-
|
|
196
|
-
## Detailed references
|
|
197
|
-
|
|
198
|
-
- `references/image_loading.md` — slide classes, backends, formats, levels,
|
|
199
|
-
coordinates, technical metadata, and privacy.
|
|
200
|
-
- `references/preprocessing.md` — stable transforms, masks/QC, stain processing,
|
|
201
|
-
pipeline execution, and leakage prevention.
|
|
202
|
-
- `references/data_management.md` — `.h5path`, manifests, datasets, provenance,
|
|
203
|
-
splits, and safe downloads.
|
|
204
|
-
- `references/multiparametric.md` — multidimensional layout, CODEX/Vectra,
|
|
205
|
-
quantification, AnnData, DeepCell/Mesmer, and network disclosure.
|
|
206
|
-
- `references/graphs.md` — instance maps, feature alignment, KNN/RAG/HACT graphs,
|
|
207
|
-
spatial units, schemas, and validation.
|
|
208
|
-
- `references/machine_learning.md` — HoVer-Net/HACTNet, local ONNX inference,
|
|
209
|
-
batching, checkpoint trust, evaluation, and model provenance.
|
|
210
|
-
|
|
211
|
-
## Primary sources
|
|
212
|
-
|
|
213
|
-
All checked 2026-07-23:
|
|
214
|
-
|
|
215
|
-
- PyPI metadata: https://pypi.org/project/pathml/3.0.5/
|
|
216
|
-
- Stable source tag: https://github.com/Dana-Farber-AIOS/pathml/tree/v3.0.5
|
|
217
|
-
- Releases: https://github.com/Dana-Farber-AIOS/pathml/releases
|
|
218
|
-
- Stable documentation: https://pathml.readthedocs.io/en/stable/
|
|
219
|
-
- Rosenthal et al. (2022), PathML toolkit:
|
|
220
|
-
https://doi.org/10.1158/1541-7786.MCR-21-0665
|
|
221
|
-
- Omar et al. (2025), multiplex workflows:
|
|
222
|
-
https://doi.org/10.1016/j.labinv.2025.104220
|
|
@@ -1,208 +0,0 @@
|
|
|
1
|
-
---
|
|
2
|
-
name: pathogen-variant-surveillance
|
|
3
|
-
description: Query live pathogen genomic surveillance data through the GenSpectrum LAPIS API to find which viral lineages are circulating now, how fast they are growing, and what mutations they carry. Use whenever a question depends on the current state of a pathogen population rather than on remembered facts - which SARS-CoV-2 variant is dominant, whether a Pango lineage is still designated or has been withdrawn, what clade or genotype of H5N1 is in a host or region, whether a PCR primer or assay target still matches circulating sequence, or how a lineage's prevalence has moved week to week. Triggers include "variant surveillance", "genomic surveillance", "what variant is circulating", "dominant variant", "Pango lineage", "lineage prevalence", "growth advantage", "SARS-CoV-2 variant", "XFG", "clade 2.3.4.4b", "H5N1 genotype", "influenza clade", "RSV/mpox/measles/dengue lineage", "CoV-Spectrum", "LAPIS", "Nextclade", "pango-designation", and any request to report what a pathogen population looks like today.
|
|
4
|
-
license: MIT
|
|
5
|
-
compatibility: Requires Python 3.11+. Scripts use only the standard library - no third-party packages. Needs network access to the public GenSpectrum LAPIS instances (lapis.cov-spectrum.org, lapis.genspectrum.org, lapis.pathoplexus.org) and to raw.githubusercontent.com for pango-designation. No API key.
|
|
6
|
-
allowed-tools: Read Write Edit Bash
|
|
7
|
-
metadata:
|
|
8
|
-
version: "1.0"
|
|
9
|
-
skill-author: K-Dense Inc.
|
|
10
|
-
last-reviewed: "2026-07-27"
|
|
11
|
-
---
|
|
12
|
-
|
|
13
|
-
# Pathogen Variant Surveillance
|
|
14
|
-
|
|
15
|
-
## When to use
|
|
16
|
-
|
|
17
|
-
Any time an answer depends on what a pathogen population looks like **now**: which lineages are
|
|
18
|
-
circulating, whether one is growing, what a lineage name currently means, or whether an assay
|
|
19
|
-
target still matches.
|
|
20
|
-
|
|
21
|
-
## The rule
|
|
22
|
-
|
|
23
|
-
**Never state what is circulating, and never write a lineage name, from memory.**
|
|
24
|
-
|
|
25
|
-
Three things go wrong at once, and only the first is an ordinary knowledge-cutoff problem:
|
|
26
|
-
|
|
27
|
-
1. **Names post-date training.** The Pango designation list carries over 6,200 names and grows
|
|
28
|
-
continuously.
|
|
29
|
-
2. **The nomenclature is a live data structure, not a convention.** `XFG` is a recombinant that
|
|
30
|
-
only resolves through `alias_key.json`; `PQ.17` unaliases to `XDV.1.5.1.1.8.1.17`. Neither
|
|
31
|
-
expansion is derivable by reasoning — the mapping is a file that changes.
|
|
32
|
-
3. **Prior knowledge gets retracted, not just outdated.** 294 names in the current
|
|
33
|
-
`lineage_notes.txt` are withdrawn or redesignated. `PC.2` is now `LF.7.9`; `XFG.20` was
|
|
34
|
-
withdrawn outright. A remembered lineage fact is not merely stale, it can be actively wrong.
|
|
35
|
-
|
|
36
|
-
Every number this skill reports is a count returned by a live instance, stamped with the data
|
|
37
|
-
version it came from.
|
|
38
|
-
|
|
39
|
-
## Scope
|
|
40
|
-
|
|
41
|
-
Surveillance data analysis for research. This skill describes sequences that were collected and
|
|
42
|
-
submitted; it does not produce clinical interpretations, outbreak-response recommendations, or
|
|
43
|
-
public-health guidance, and sequence counts are not case counts.
|
|
44
|
-
|
|
45
|
-
## Instances
|
|
46
|
-
|
|
47
|
-
One API shape covers every pathogen. `--instance` names a verified deployment; `--base-url`
|
|
48
|
-
reaches any other LAPIS instance.
|
|
49
|
-
|
|
50
|
-
| Instance | Host | Lineage column | Indexed |
|
|
51
|
-
| --- | --- | --- | --- |
|
|
52
|
-
| `sars-cov-2` | lapis.cov-spectrum.org (open GenBank data) | `pangoLineage` | yes |
|
|
53
|
-
| `h5n1`, `h3n2`, `h1n1pdm`, `influenza-a` | lapis.genspectrum.org | `clade` | no |
|
|
54
|
-
| `rsv-a`, `rsv-b`, `mpox`, `measles`, `dengue`, `west-nile`, `hmpv`, `ebola-zaire`, `ebola-sudan`, `cchf` | lapis.pathoplexus.org | varies | varies |
|
|
55
|
-
|
|
56
|
-
**Field names differ per instance and are never assumed.** Every script reads
|
|
57
|
-
`/sample/databaseConfig` at run time and picks the collection-date, submission-date and lineage
|
|
58
|
-
columns from what the instance actually declares. `dateFrom=` is correct on SARS-CoV-2 and a hard
|
|
59
|
-
400 on H5N1, whose collection date is `sampleCollectionDateRangeLower`.
|
|
60
|
-
|
|
61
|
-
## Scripts
|
|
62
|
-
|
|
63
|
-
```bash
|
|
64
|
-
cd skills/pathogen-variant-surveillance/scripts
|
|
65
|
-
```
|
|
66
|
-
|
|
67
|
-
| Script | Question answered |
|
|
68
|
-
| --- | --- |
|
|
69
|
-
| `resolve_lineage.py` | Does this name still exist, what does it expand to, what is it descended from? |
|
|
70
|
-
| `lineage_prevalence.py` | What share of sequences is this lineage, week by week, and is it growing? |
|
|
71
|
-
| `mutation_profile.py` | What mutations does it carry, and how does it differ from another lineage? |
|
|
72
|
-
| `reporting_lag.py` | How far back does the data have to go before it can be trusted? |
|
|
73
|
-
|
|
74
|
-
All four take `--format table|tsv|json` and print provenance (instance, data version, resolved
|
|
75
|
-
field names, filters) to stderr, so `> out.tsv` keeps the data clean and the provenance visible.
|
|
76
|
-
|
|
77
|
-
### Start from the data, not from a remembered list
|
|
78
|
-
|
|
79
|
-
```bash
|
|
80
|
-
# no names: discover what is actually circulating in the window
|
|
81
|
-
python3 lineage_prevalence.py --top 5 --where country=USA --weeks 12
|
|
82
|
-
```
|
|
83
|
-
|
|
84
|
-
> note: discovered the 5 most common pangoLineage values in the window:
|
|
85
|
-
> XFG.1.1, XFG.23.1.3, PY.1.1.1, XFJ.3.1.2, PQ.17
|
|
86
|
-
|
|
87
|
-
This is the right first command for "what is circulating". Naming lineages up front presumes you
|
|
88
|
-
already know which ones matter, which is the assumption this skill exists to remove.
|
|
89
|
-
|
|
90
|
-
### Check a name before using it
|
|
91
|
-
|
|
92
|
-
```bash
|
|
93
|
-
python3 resolve_lineage.py XFG.23.1.3 PQ.17 PC.2 NOTALINEAGE
|
|
94
|
-
```
|
|
95
|
-
|
|
96
|
-
```
|
|
97
|
-
query status unaliased parent recombinant_of descendants sequences detail
|
|
98
|
-
XFG.23.1.3 current XFG.23.1.3 XFG.23.1 LF.7+LP.8.1.2 6 317 S:A1174V, on C29137T branch
|
|
99
|
-
PQ.17 current XDV.1.5.1.1.8.1.17 NB.1.8.1 23 931 Alias of XDV.1.5.1.1.8.1.17
|
|
100
|
-
PC.2 withdrawn B.1.1.529.2.86.1.1.16.1.7.2.1.2 LF.7.2.1 4 25 now LF.7.9; Redesignated as LF.7.9
|
|
101
|
-
NOTALINEAGE unknown NOTALINEAGE 0 n/a no such name in the live nomenclature
|
|
102
|
-
```
|
|
103
|
-
|
|
104
|
-
(`detail` abridged; each real row also cites the lineage proposal it came from.)
|
|
105
|
-
|
|
106
|
-
Exit code is 1 if any name is withdrawn or unknown, so it gates a manuscript's lineage list.
|
|
107
|
-
Note `PC.2`: withdrawn upstream, yet 25 sequences still carry the label because the instance's
|
|
108
|
-
assignments lag designation. Both facts are true and both matter.
|
|
109
|
-
|
|
110
|
-
### Prevalence and growth
|
|
111
|
-
|
|
112
|
-
```bash
|
|
113
|
-
python3 lineage_prevalence.py "XFG.1.1*" "XFJ*" --where country=USA --weeks 16 --growth
|
|
114
|
-
```
|
|
115
|
-
|
|
116
|
-
```
|
|
117
|
-
lineage week n total proportion ci_low ci_high coverage
|
|
118
|
-
XFG.1.1* 2026-05-04 42 80 0.5250 0.4170 0.6308 ok
|
|
119
|
-
XFG.1.1* 2026-06-15 3 49 0.0612 0.0210 0.1652 ok
|
|
120
|
-
XFG.1.1* 2026-06-29 1 30 0.0333 0.0059 0.1667 low
|
|
121
|
-
XFG.1.1* 2026-07-13 0 0 low
|
|
122
|
-
```
|
|
123
|
-
|
|
124
|
-
Proportions carry Wilson intervals because surveillance weeks are small. Weeks whose denominator
|
|
125
|
-
has not filled in yet are flagged `low` and excluded from the growth fit unless
|
|
126
|
-
`--include-incomplete`.
|
|
127
|
-
|
|
128
|
-
The window is widened to whole ISO weeks, and says so when it does. A window starting mid-week
|
|
129
|
-
would give a first row covering three days and a last row covering four, neither comparable to the
|
|
130
|
-
full weeks between them.
|
|
131
|
-
|
|
132
|
-
`--growth` reports a weighted least-squares slope of log-odds against time. It is **descriptive**:
|
|
133
|
-
it absorbs every change in who is sequencing, where, and how fast they report. It is not a fitness
|
|
134
|
-
or transmissibility estimate. No slope is printed for a lineage with too few observations — see the
|
|
135
|
-
trap table for why that guard exists.
|
|
136
|
-
|
|
137
|
-
### Mutations, and whether an assay still matches
|
|
138
|
-
|
|
139
|
-
```bash
|
|
140
|
-
python3 mutation_profile.py "XFJ*" --versus "XFG*" --gene S --since 2026-01-01
|
|
141
|
-
```
|
|
142
|
-
|
|
143
|
-
```
|
|
144
|
-
mutation gene position verdict prop_a prop_b n_a n_b
|
|
145
|
-
S:L441R S 441 gained 1.000 0.000 66 0
|
|
146
|
-
S:A475V S 475 gained 1.000 0.000 68 0
|
|
147
|
-
S:K444R S 444 lost 0.000 0.996 0 5031
|
|
148
|
-
S:Q493E S 493 lost 0.000 0.998 0 5359
|
|
149
|
-
```
|
|
150
|
-
|
|
151
|
-
Works the same on a segmented genome — `--instance h5n1 --gene HA` or `--gene seg4`. Use
|
|
152
|
-
`--nucleotide` for primer and probe questions, where the codon is not the unit that matters.
|
|
153
|
-
|
|
154
|
-
### Decide how far back to trust
|
|
155
|
-
|
|
156
|
-
```bash
|
|
157
|
-
python3 reporting_lag.py --where country=USA
|
|
158
|
-
```
|
|
159
|
-
|
|
160
|
-
```
|
|
161
|
-
lag_days mean_complete min_complete max_complete cohorts
|
|
162
|
-
14 0.456 0.332 0.557 6
|
|
163
|
-
30 0.677 0.580 0.822 6
|
|
164
|
-
60 0.868 0.802 0.949 6
|
|
165
|
-
90 0.939 0.916 1.000 6
|
|
166
|
-
```
|
|
167
|
-
|
|
168
|
-
> 90% of a cohort has arrived by 90 days. Trust collection dates up to 2026-04-28; treat anything
|
|
169
|
-
> later as provisional.
|
|
170
|
-
|
|
171
|
-
Run this **before** quoting any recent prevalence. The curve differs sharply by pathogen and
|
|
172
|
-
country: on H5N1 the same measurement returns 0% complete at 14 days and 15% at 30 days, so a
|
|
173
|
-
"current" H5N1 picture is effectively blind for two months.
|
|
174
|
-
|
|
175
|
-
## Traps that produce silently wrong answers
|
|
176
|
-
|
|
177
|
-
All verified against the live API on 2026-07-27. These are why this skill ships scripts rather
|
|
178
|
-
than a recipe; full detail in `references/lapis-api.md`.
|
|
179
|
-
|
|
180
|
-
| Trap | Consequence |
|
|
181
|
-
| --- | --- |
|
|
182
|
-
| A bare lineage name excludes its descendants | `pangoLineage=XFG` returns 4 sequences; `XFG*` returns 640 |
|
|
183
|
-
| A trailing `*` needs a lineage index | On H5N1 `clade=2.3.4.4b` returns 62,413 and `clade=2.3.4.4b*` returns **0** — the same syntax, the opposite meaning |
|
|
184
|
-
| Field names are per-instance | `dateFrom` is a 400 on H5N1; the collection date is `sampleCollectionDateRangeLower` |
|
|
185
|
-
| Only `date`-typed fields take ranges | H5N1 types `sampleCollectionDate` as a string, so it has no `From`/`To` keys at all |
|
|
186
|
-
| Recent weeks are not a sample of what circulated | They are a sample of whoever reports fastest; only 29% of a US cohort arrives within 7 days |
|
|
187
|
-
| LAPIS roots recombinants | Asking it for `XFG`'s parents returns nothing; only `alias_key.json` records `XFG = LF.7 + LP.8.1.2` |
|
|
188
|
-
| Withdrawn names persist in the data | `PC.2` was redesignated `LF.7.9` upstream while sequences still carry `PC.2` |
|
|
189
|
-
| An unknown name fails loudly only when indexed | Indexed columns reject a typo with a 400; unindexed columns answer `0` |
|
|
190
|
-
| Mutation `proportion` is over `coverage` | Not over all matching sequences — a poorly covered site can show 1.000 on very few reads |
|
|
191
|
-
| `/sample/aggregated` rejects `limit`/`orderBy` | The result has no inherent ordering; sort client-side |
|
|
192
|
-
|
|
193
|
-
## Reporting results
|
|
194
|
-
|
|
195
|
-
State the instance, the data version, the filters, and the window — a prevalence figure without
|
|
196
|
-
them cannot be reproduced, because the underlying database changes daily. Give counts alongside
|
|
197
|
-
proportions, quote the interval, and say explicitly when a window is too recent to support an
|
|
198
|
-
estimate. "No reliable estimate for the last six weeks" is a legitimate and often correct answer.
|
|
199
|
-
|
|
200
|
-
## References
|
|
201
|
-
|
|
202
|
-
- `references/lapis-api.md` — endpoints, filter grammar, per-instance schema differences, the
|
|
203
|
-
instance registry, and every verified trap in full.
|
|
204
|
-
- `references/lineage-nomenclature.md` — Pango aliases and recombinants, designation churn,
|
|
205
|
-
Nextstrain clades, WHO labels, influenza clades, H5N1 clades and genotypes, and how the naming
|
|
206
|
-
systems map onto each other.
|
|
207
|
-
- `references/surveillance-caveats.md` — reporting lag, sampling and ascertainment bias, choosing
|
|
208
|
-
a denominator, interval and growth interpretation, and the conclusions this data cannot support.
|