finishm 0.0.1

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Files changed (554) hide show
  1. checksums.yaml +7 -0
  2. data/.document +5 -0
  3. data/.gitmodules +3 -0
  4. data/.rspec +1 -0
  5. data/Gemfile +31 -0
  6. data/LICENSE.txt +20 -0
  7. data/README.md +59 -0
  8. data/Rakefile +51 -0
  9. data/VERSION +1 -0
  10. data/bin/assembly_visualiser +106 -0
  11. data/bin/check_primer_combinations.rb +73 -0
  12. data/bin/contig_joiner.rb +244 -0
  13. data/bin/contigs_against_assembly.rb +153 -0
  14. data/bin/finishm +143 -0
  15. data/bin/finishm_assembler +55 -0
  16. data/bin/finishm_gap_closer.rb +241 -0
  17. data/bin/kmer_abundance_file_tool.rb +49 -0
  18. data/bin/kmer_pattern_to_assembly.rb +377 -0
  19. data/bin/kmer_profile_finder.rb +92 -0
  20. data/bin/kmers_count_parse.d +52 -0
  21. data/bin/kmers_count_tabulate.d +123 -0
  22. data/bin/kmers_count_tabulate.rb +84 -0
  23. data/bin/pcr_result_parser.rb +108 -0
  24. data/bin/primer_finder.rb +119 -0
  25. data/bin/read_selection_by_kmer.d +174 -0
  26. data/bin/scaffold_by_pattern.rb +119 -0
  27. data/bin/scaffold_connection_possibilities_to_knowns.rb +193 -0
  28. data/bin/scaffold_end_coverages.rb +69 -0
  29. data/bin/trail_validator.rb +84 -0
  30. data/ext/mkrf_conf.rb +56 -0
  31. data/ext/src/Makefile +140 -0
  32. data/ext/src/src/allocArray.c +305 -0
  33. data/ext/src/src/allocArray.h +86 -0
  34. data/ext/src/src/autoOpen.c +107 -0
  35. data/ext/src/src/autoOpen.h +18 -0
  36. data/ext/src/src/binarySequences.c +813 -0
  37. data/ext/src/src/binarySequences.h +125 -0
  38. data/ext/src/src/concatenatedGraph.c +233 -0
  39. data/ext/src/src/concatenatedGraph.h +30 -0
  40. data/ext/src/src/concatenatedPreGraph.c +262 -0
  41. data/ext/src/src/concatenatedPreGraph.h +29 -0
  42. data/ext/src/src/correctedGraph.c +2643 -0
  43. data/ext/src/src/correctedGraph.h +32 -0
  44. data/ext/src/src/dfib.c +509 -0
  45. data/ext/src/src/dfib.h +69 -0
  46. data/ext/src/src/dfibHeap.c +89 -0
  47. data/ext/src/src/dfibHeap.h +39 -0
  48. data/ext/src/src/dfibpriv.h +105 -0
  49. data/ext/src/src/fib.c +628 -0
  50. data/ext/src/src/fib.h +78 -0
  51. data/ext/src/src/fibHeap.c +79 -0
  52. data/ext/src/src/fibHeap.h +41 -0
  53. data/ext/src/src/fibpriv.h +110 -0
  54. data/ext/src/src/globals.h +154 -0
  55. data/ext/src/src/graph.c +3932 -0
  56. data/ext/src/src/graph.h +233 -0
  57. data/ext/src/src/graphReConstruction.c +1472 -0
  58. data/ext/src/src/graphReConstruction.h +30 -0
  59. data/ext/src/src/graphStats.c +2167 -0
  60. data/ext/src/src/graphStats.h +72 -0
  61. data/ext/src/src/graphStructures.h +52 -0
  62. data/ext/src/src/kmer.c +652 -0
  63. data/ext/src/src/kmer.h +73 -0
  64. data/ext/src/src/kmerOccurenceTable.c +236 -0
  65. data/ext/src/src/kmerOccurenceTable.h +44 -0
  66. data/ext/src/src/kseq.h +223 -0
  67. data/ext/src/src/locallyCorrectedGraph.c +557 -0
  68. data/ext/src/src/locallyCorrectedGraph.h +40 -0
  69. data/ext/src/src/passageMarker.c +677 -0
  70. data/ext/src/src/passageMarker.h +137 -0
  71. data/ext/src/src/preGraph.c +1717 -0
  72. data/ext/src/src/preGraph.h +106 -0
  73. data/ext/src/src/preGraphConstruction.c +990 -0
  74. data/ext/src/src/preGraphConstruction.h +26 -0
  75. data/ext/src/src/probe_node_finder.c +84 -0
  76. data/ext/src/src/probe_node_finder.h +6 -0
  77. data/ext/src/src/readCoherentGraph.c +557 -0
  78. data/ext/src/src/readCoherentGraph.h +30 -0
  79. data/ext/src/src/readSet.c +1734 -0
  80. data/ext/src/src/readSet.h +67 -0
  81. data/ext/src/src/readToNode.c +218 -0
  82. data/ext/src/src/readToNode.h +35 -0
  83. data/ext/src/src/recycleBin.c +199 -0
  84. data/ext/src/src/recycleBin.h +58 -0
  85. data/ext/src/src/roadMap.c +342 -0
  86. data/ext/src/src/roadMap.h +65 -0
  87. data/ext/src/src/run.c +318 -0
  88. data/ext/src/src/run.h +52 -0
  89. data/ext/src/src/run2.c +744 -0
  90. data/ext/src/src/runReadToNode.c +29 -0
  91. data/ext/src/src/scaffold.c +1876 -0
  92. data/ext/src/src/scaffold.h +64 -0
  93. data/ext/src/src/shortReadPairs.c +1243 -0
  94. data/ext/src/src/shortReadPairs.h +32 -0
  95. data/ext/src/src/splay.c +259 -0
  96. data/ext/src/src/splay.h +43 -0
  97. data/ext/src/src/splayTable.c +1315 -0
  98. data/ext/src/src/splayTable.h +31 -0
  99. data/ext/src/src/tightString.c +362 -0
  100. data/ext/src/src/tightString.h +82 -0
  101. data/ext/src/src/utility.c +199 -0
  102. data/ext/src/src/utility.h +98 -0
  103. data/ext/src/third-party/zlib-1.2.3/ChangeLog +855 -0
  104. data/ext/src/third-party/zlib-1.2.3/FAQ +339 -0
  105. data/ext/src/third-party/zlib-1.2.3/INDEX +51 -0
  106. data/ext/src/third-party/zlib-1.2.3/Makefile +154 -0
  107. data/ext/src/third-party/zlib-1.2.3/Makefile.in +154 -0
  108. data/ext/src/third-party/zlib-1.2.3/README +125 -0
  109. data/ext/src/third-party/zlib-1.2.3/adler32.c +149 -0
  110. data/ext/src/third-party/zlib-1.2.3/adler32.o +0 -0
  111. data/ext/src/third-party/zlib-1.2.3/algorithm.txt +209 -0
  112. data/ext/src/third-party/zlib-1.2.3/amiga/Makefile.pup +66 -0
  113. data/ext/src/third-party/zlib-1.2.3/amiga/Makefile.sas +65 -0
  114. data/ext/src/third-party/zlib-1.2.3/as400/bndsrc +132 -0
  115. data/ext/src/third-party/zlib-1.2.3/as400/compile.clp +123 -0
  116. data/ext/src/third-party/zlib-1.2.3/as400/readme.txt +111 -0
  117. data/ext/src/third-party/zlib-1.2.3/as400/zlib.inc +331 -0
  118. data/ext/src/third-party/zlib-1.2.3/compress.c +79 -0
  119. data/ext/src/third-party/zlib-1.2.3/compress.o +0 -0
  120. data/ext/src/third-party/zlib-1.2.3/configure +459 -0
  121. data/ext/src/third-party/zlib-1.2.3/contrib/README.contrib +71 -0
  122. data/ext/src/third-party/zlib-1.2.3/contrib/ada/buffer_demo.adb +106 -0
  123. data/ext/src/third-party/zlib-1.2.3/contrib/ada/mtest.adb +156 -0
  124. data/ext/src/third-party/zlib-1.2.3/contrib/ada/read.adb +156 -0
  125. data/ext/src/third-party/zlib-1.2.3/contrib/ada/readme.txt +65 -0
  126. data/ext/src/third-party/zlib-1.2.3/contrib/ada/test.adb +463 -0
  127. data/ext/src/third-party/zlib-1.2.3/contrib/ada/zlib-streams.adb +225 -0
  128. data/ext/src/third-party/zlib-1.2.3/contrib/ada/zlib-streams.ads +114 -0
  129. data/ext/src/third-party/zlib-1.2.3/contrib/ada/zlib-thin.adb +141 -0
  130. data/ext/src/third-party/zlib-1.2.3/contrib/ada/zlib-thin.ads +450 -0
  131. data/ext/src/third-party/zlib-1.2.3/contrib/ada/zlib.adb +701 -0
  132. data/ext/src/third-party/zlib-1.2.3/contrib/ada/zlib.ads +328 -0
  133. data/ext/src/third-party/zlib-1.2.3/contrib/ada/zlib.gpr +20 -0
  134. data/ext/src/third-party/zlib-1.2.3/contrib/asm586/README.586 +43 -0
  135. data/ext/src/third-party/zlib-1.2.3/contrib/asm586/match.S +364 -0
  136. data/ext/src/third-party/zlib-1.2.3/contrib/asm686/README.686 +34 -0
  137. data/ext/src/third-party/zlib-1.2.3/contrib/asm686/match.S +329 -0
  138. data/ext/src/third-party/zlib-1.2.3/contrib/blast/Makefile +8 -0
  139. data/ext/src/third-party/zlib-1.2.3/contrib/blast/README +4 -0
  140. data/ext/src/third-party/zlib-1.2.3/contrib/blast/blast.c +444 -0
  141. data/ext/src/third-party/zlib-1.2.3/contrib/blast/blast.h +71 -0
  142. data/ext/src/third-party/zlib-1.2.3/contrib/blast/test.pk +0 -0
  143. data/ext/src/third-party/zlib-1.2.3/contrib/blast/test.txt +1 -0
  144. data/ext/src/third-party/zlib-1.2.3/contrib/delphi/ZLib.pas +557 -0
  145. data/ext/src/third-party/zlib-1.2.3/contrib/delphi/ZLibConst.pas +11 -0
  146. data/ext/src/third-party/zlib-1.2.3/contrib/delphi/readme.txt +76 -0
  147. data/ext/src/third-party/zlib-1.2.3/contrib/delphi/zlibd32.mak +93 -0
  148. data/ext/src/third-party/zlib-1.2.3/contrib/dotzlib/DotZLib.build +33 -0
  149. data/ext/src/third-party/zlib-1.2.3/contrib/dotzlib/DotZLib.chm +0 -0
  150. data/ext/src/third-party/zlib-1.2.3/contrib/dotzlib/DotZLib.sln +21 -0
  151. data/ext/src/third-party/zlib-1.2.3/contrib/dotzlib/DotZLib/AssemblyInfo.cs +58 -0
  152. data/ext/src/third-party/zlib-1.2.3/contrib/dotzlib/DotZLib/ChecksumImpl.cs +202 -0
  153. data/ext/src/third-party/zlib-1.2.3/contrib/dotzlib/DotZLib/CircularBuffer.cs +83 -0
  154. data/ext/src/third-party/zlib-1.2.3/contrib/dotzlib/DotZLib/CodecBase.cs +198 -0
  155. data/ext/src/third-party/zlib-1.2.3/contrib/dotzlib/DotZLib/Deflater.cs +106 -0
  156. data/ext/src/third-party/zlib-1.2.3/contrib/dotzlib/DotZLib/DotZLib.cs +288 -0
  157. data/ext/src/third-party/zlib-1.2.3/contrib/dotzlib/DotZLib/DotZLib.csproj +141 -0
  158. data/ext/src/third-party/zlib-1.2.3/contrib/dotzlib/DotZLib/GZipStream.cs +301 -0
  159. data/ext/src/third-party/zlib-1.2.3/contrib/dotzlib/DotZLib/Inflater.cs +105 -0
  160. data/ext/src/third-party/zlib-1.2.3/contrib/dotzlib/DotZLib/UnitTests.cs +274 -0
  161. data/ext/src/third-party/zlib-1.2.3/contrib/dotzlib/LICENSE_1_0.txt +23 -0
  162. data/ext/src/third-party/zlib-1.2.3/contrib/dotzlib/readme.txt +58 -0
  163. data/ext/src/third-party/zlib-1.2.3/contrib/infback9/README +1 -0
  164. data/ext/src/third-party/zlib-1.2.3/contrib/infback9/infback9.c +608 -0
  165. data/ext/src/third-party/zlib-1.2.3/contrib/infback9/infback9.h +37 -0
  166. data/ext/src/third-party/zlib-1.2.3/contrib/infback9/inffix9.h +107 -0
  167. data/ext/src/third-party/zlib-1.2.3/contrib/infback9/inflate9.h +47 -0
  168. data/ext/src/third-party/zlib-1.2.3/contrib/infback9/inftree9.c +323 -0
  169. data/ext/src/third-party/zlib-1.2.3/contrib/infback9/inftree9.h +55 -0
  170. data/ext/src/third-party/zlib-1.2.3/contrib/inflate86/inffas86.c +1157 -0
  171. data/ext/src/third-party/zlib-1.2.3/contrib/inflate86/inffast.S +1368 -0
  172. data/ext/src/third-party/zlib-1.2.3/contrib/iostream/test.cpp +24 -0
  173. data/ext/src/third-party/zlib-1.2.3/contrib/iostream/zfstream.cpp +329 -0
  174. data/ext/src/third-party/zlib-1.2.3/contrib/iostream/zfstream.h +128 -0
  175. data/ext/src/third-party/zlib-1.2.3/contrib/iostream2/zstream.h +307 -0
  176. data/ext/src/third-party/zlib-1.2.3/contrib/iostream2/zstream_test.cpp +25 -0
  177. data/ext/src/third-party/zlib-1.2.3/contrib/iostream3/README +35 -0
  178. data/ext/src/third-party/zlib-1.2.3/contrib/iostream3/TODO +17 -0
  179. data/ext/src/third-party/zlib-1.2.3/contrib/iostream3/test.cc +50 -0
  180. data/ext/src/third-party/zlib-1.2.3/contrib/iostream3/zfstream.cc +479 -0
  181. data/ext/src/third-party/zlib-1.2.3/contrib/iostream3/zfstream.h +466 -0
  182. data/ext/src/third-party/zlib-1.2.3/contrib/masm686/match.asm +413 -0
  183. data/ext/src/third-party/zlib-1.2.3/contrib/masmx64/bld_ml64.bat +2 -0
  184. data/ext/src/third-party/zlib-1.2.3/contrib/masmx64/gvmat64.asm +513 -0
  185. data/ext/src/third-party/zlib-1.2.3/contrib/masmx64/gvmat64.obj +0 -0
  186. data/ext/src/third-party/zlib-1.2.3/contrib/masmx64/inffas8664.c +186 -0
  187. data/ext/src/third-party/zlib-1.2.3/contrib/masmx64/inffasx64.asm +392 -0
  188. data/ext/src/third-party/zlib-1.2.3/contrib/masmx64/inffasx64.obj +0 -0
  189. data/ext/src/third-party/zlib-1.2.3/contrib/masmx64/readme.txt +28 -0
  190. data/ext/src/third-party/zlib-1.2.3/contrib/masmx86/bld_ml32.bat +2 -0
  191. data/ext/src/third-party/zlib-1.2.3/contrib/masmx86/gvmat32.asm +972 -0
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  193. data/ext/src/third-party/zlib-1.2.3/contrib/masmx86/gvmat32c.c +62 -0
  194. data/ext/src/third-party/zlib-1.2.3/contrib/masmx86/inffas32.asm +1083 -0
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  196. data/ext/src/third-party/zlib-1.2.3/contrib/masmx86/mkasm.bat +3 -0
  197. data/ext/src/third-party/zlib-1.2.3/contrib/masmx86/readme.txt +21 -0
  198. data/ext/src/third-party/zlib-1.2.3/contrib/minizip/ChangeLogUnzip +67 -0
  199. data/ext/src/third-party/zlib-1.2.3/contrib/minizip/Makefile +25 -0
  200. data/ext/src/third-party/zlib-1.2.3/contrib/minizip/crypt.h +132 -0
  201. data/ext/src/third-party/zlib-1.2.3/contrib/minizip/ioapi.c +177 -0
  202. data/ext/src/third-party/zlib-1.2.3/contrib/minizip/ioapi.h +75 -0
  203. data/ext/src/third-party/zlib-1.2.3/contrib/minizip/iowin32.c +270 -0
  204. data/ext/src/third-party/zlib-1.2.3/contrib/minizip/iowin32.h +21 -0
  205. data/ext/src/third-party/zlib-1.2.3/contrib/minizip/miniunz.c +585 -0
  206. data/ext/src/third-party/zlib-1.2.3/contrib/minizip/minizip.c +420 -0
  207. data/ext/src/third-party/zlib-1.2.3/contrib/minizip/mztools.c +281 -0
  208. data/ext/src/third-party/zlib-1.2.3/contrib/minizip/mztools.h +31 -0
  209. data/ext/src/third-party/zlib-1.2.3/contrib/minizip/unzip.c +1598 -0
  210. data/ext/src/third-party/zlib-1.2.3/contrib/minizip/unzip.h +354 -0
  211. data/ext/src/third-party/zlib-1.2.3/contrib/minizip/zip.c +1219 -0
  212. data/ext/src/third-party/zlib-1.2.3/contrib/minizip/zip.h +235 -0
  213. data/ext/src/third-party/zlib-1.2.3/contrib/pascal/example.pas +599 -0
  214. data/ext/src/third-party/zlib-1.2.3/contrib/pascal/readme.txt +76 -0
  215. data/ext/src/third-party/zlib-1.2.3/contrib/pascal/zlibd32.mak +93 -0
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  217. data/ext/src/third-party/zlib-1.2.3/contrib/puff/Makefile +8 -0
  218. data/ext/src/third-party/zlib-1.2.3/contrib/puff/README +63 -0
  219. data/ext/src/third-party/zlib-1.2.3/contrib/puff/puff.c +837 -0
  220. data/ext/src/third-party/zlib-1.2.3/contrib/puff/puff.h +31 -0
  221. data/ext/src/third-party/zlib-1.2.3/contrib/puff/zeros.raw +0 -0
  222. data/ext/src/third-party/zlib-1.2.3/contrib/testzlib/testzlib.c +275 -0
  223. data/ext/src/third-party/zlib-1.2.3/contrib/testzlib/testzlib.txt +10 -0
  224. data/ext/src/third-party/zlib-1.2.3/contrib/untgz/Makefile +14 -0
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  226. data/ext/src/third-party/zlib-1.2.3/contrib/untgz/untgz.c +674 -0
  227. data/ext/src/third-party/zlib-1.2.3/contrib/vstudio/readme.txt +73 -0
  228. data/ext/src/third-party/zlib-1.2.3/contrib/vstudio/vc7/miniunz.vcproj +126 -0
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  245. data/ext/src/third-party/zlib-1.2.3/crc32.c +423 -0
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  248. data/ext/src/third-party/zlib-1.2.3/deflate.c +1736 -0
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  251. data/ext/src/third-party/zlib-1.2.3/example +0 -0
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  253. data/ext/src/third-party/zlib-1.2.3/examples/README.examples +42 -0
  254. data/ext/src/third-party/zlib-1.2.3/examples/fitblk.c +233 -0
  255. data/ext/src/third-party/zlib-1.2.3/examples/gun.c +693 -0
  256. data/ext/src/third-party/zlib-1.2.3/examples/gzappend.c +500 -0
  257. data/ext/src/third-party/zlib-1.2.3/examples/gzjoin.c +448 -0
  258. data/ext/src/third-party/zlib-1.2.3/examples/gzlog.c +413 -0
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  523. data/spec/dijkstra_spec.rb +143 -0
  524. data/spec/explore_spec.rb +29 -0
  525. data/spec/fluffer_spec.rb +155 -0
  526. data/spec/gapfiller_spec.rb +107 -0
  527. data/spec/graph_explorer_spec.rb +475 -0
  528. data/spec/graph_generator_spec.rb +99 -0
  529. data/spec/height_finder_spec.rb +306 -0
  530. data/spec/kmer_abundance_pattern_spec.rb +56 -0
  531. data/spec/kmer_coverage_based_path_filter_spec.rb +73 -0
  532. data/spec/kmer_profile_finder_spec.rb +38 -0
  533. data/spec/kmers_count_tabulate_spec.rb +120 -0
  534. data/spec/oriented_node_trail_spec.rb +221 -0
  535. data/spec/paired_end_neighbours_spec.rb +126 -0
  536. data/spec/paths_between_nodes_spec.rb +349 -0
  537. data/spec/priner_spec.rb +7 -0
  538. data/spec/read_input_spec.rb +23 -0
  539. data/spec/read_selection_by_kmer_spec.rb +166 -0
  540. data/spec/read_to_node_spec.rb +35 -0
  541. data/spec/roundup_spec.rb +366 -0
  542. data/spec/scaffold_breaker_spec.rb +144 -0
  543. data/spec/sequence_spec.rb +43 -0
  544. data/spec/single_coherent_paths_between_nodes_spec.rb +492 -0
  545. data/spec/single_coherent_wanderer_spec.rb +120 -0
  546. data/spec/single_ended_assembler_spec.rb +398 -0
  547. data/spec/spec_helper.rb +310 -0
  548. data/spec/velvet_graph_sequence_extractor_spec.rb +80 -0
  549. data/spec/visualise_spec.rb +105 -0
  550. data/spec/wander_spec.rb +119 -0
  551. data/spec/watch_for_changes.sh +16 -0
  552. data/validation/fasta_compare.rb +72 -0
  553. data/validation/gapfill_simulate_perfect.rb +108 -0
  554. metadata +899 -0
@@ -0,0 +1,69 @@
1
+ #!/usr/bin/env ruby
2
+
3
+ require 'optparse'
4
+ require 'bio-logger'
5
+ require 'bio-samtools'
6
+ require 'bio'
7
+
8
+ if __FILE__ == $0 #needs to be removed if this script is distributed as part of a rubygem
9
+ SCRIPT_NAME = File.basename(__FILE__); LOG_NAME = SCRIPT_NAME.gsub('.rb','')
10
+
11
+ # Parse command line options into the options hash
12
+ options = {
13
+ :logger => 'stderr',
14
+ :contig_end_length => 200,
15
+ }
16
+ o = OptionParser.new do |opts|
17
+ opts.banner = "
18
+ Usage: #{SCRIPT_NAME} <arguments>
19
+
20
+ Takes a sorted, indexed BAM file and outputs the coverages of each of the reference sequences\n\n"
21
+
22
+ opts.on("-b", "--bam BAM_FILE", "BAM file that defines overall mapping/coverage [required]") do |arg|
23
+ options[:bam_file] = arg
24
+ end
25
+ opts.on("-f", "--reference-fasta FASTA_FILE", "FASTA file of the reference [required]") do |arg|
26
+ options[:fasta_file] = arg
27
+ end
28
+
29
+ opts.on("-l", "--end-length LENGTH", "How far from the end to count [default: #{options[:contig_end_length]}]") do |arg|
30
+ options[:contig_end_length] = arg.to_i
31
+ raise "Inappropriate end length detected, I need a positive number, found #{options[:contig_end_length]} parsed from #{arg}" if options[:contig_end_length] < 1
32
+ end
33
+
34
+ # logger options
35
+ opts.separator "\n\tVerbosity:\n\n"
36
+ opts.on("-q", "--quiet", "Run quietly, set logging to ERROR level [default INFO]") {Bio::Log::CLI.trace('error')}
37
+ opts.on("--logger filename",String,"Log to file [default #{options[:logger]}]") { |name| options[:logger] = name}
38
+ opts.on("--trace options",String,"Set log level [default INFO]. e.g. '--trace debug' to set logging level to DEBUG"){|s| Bio::Log::CLI.trace(s)}
39
+ end; o.parse!
40
+ if ARGV.length != 0 or options[:bam_file].nil? or options[:fasta_file].nil?
41
+ $stderr.puts o
42
+ exit 1
43
+ end
44
+ # Setup logging. bio-logger defaults to STDERR not STDOUT, I disagree
45
+ Bio::Log::CLI.logger(options[:logger]); log = Bio::Log::LoggerPlus.new(LOG_NAME); Bio::Log::CLI.configure(LOG_NAME)
46
+
47
+
48
+ # open the BAM file for reading
49
+ bam = Bio::DB::Sam.new(:bam => options[:bam_file], :fasta => options[:fasta_file])
50
+ bam.open
51
+ puts %w(Reference StartCoverage EndCoverage).join("\t")
52
+ contigs_file = Bio::FlatFile.auto(options[:fasta_file])
53
+ contigs_file.each_entry do |record|
54
+ ref = record.definition
55
+ ref_length = record.length
56
+ #bam.each_reference do |ref, ref_length| #currently commented out because there is extraneous output on STDOUT if you use this
57
+ next if ref == '*' #Ignore umapped reads
58
+ # Coverage of the start
59
+ end_length = options[:contig_end_length]
60
+ end_length = ref_length if ref_length < options[:contig_end_length]
61
+
62
+ cov_start = bam.average_coverage(ref, 1, end_length)
63
+ cov_end = bam.average_coverage(ref, ref_length-end_length+1, end_length)
64
+ puts [
65
+ ref, cov_start, cov_end
66
+ ].join("\t")
67
+ end
68
+ exit
69
+ end #end if running as a script
@@ -0,0 +1,84 @@
1
+ #!/usr/bin/env ruby
2
+
3
+ require 'optparse'
4
+ require 'bio-logger'
5
+ require 'pp'
6
+
7
+ SCRIPT_NAME = File.basename(__FILE__); LOG_NAME = 'finishm'
8
+ $:.unshift File.join(File.dirname(__FILE__),'..','lib')
9
+ require 'priner'
10
+
11
+ # Parse command line options into the options hash
12
+ options = {
13
+ :logger => 'stderr',
14
+ :log_level => 'info',
15
+ }
16
+
17
+ o = OptionParser.new do |opts|
18
+ opts.banner = "
19
+ Usage: #{SCRIPT_NAME} --trails <trail(s) fasta> --kmer-abundances <abundances.csv>
20
+
21
+ Given an input kmer set of sequences then abundances space separated file, and a threshold, print out how many kmers are unique to different subsets of columns\n\n"
22
+
23
+ opts.on("--trails FASTA_FILE", "fasta file of trail(s) to be tested [required]") do |arg|
24
+ options[:trails_fasta_file] = arg
25
+ end
26
+ opts.on("--kmer-abundances FILE", "kmer multiple abundance file [required]") do |arg|
27
+ options[:kmer_multiple_abundance_file] = arg
28
+ end
29
+
30
+ #opts.separator "\nOptional arguments:\n\n"
31
+ opts.on("--output-kmer-coverages FILE", "output kmer coverages across each library across the contigs default: don't output]") do |arg|
32
+ options[:output_trail_kmer_coverage_file] = arg
33
+ end
34
+
35
+ # logger options
36
+ opts.separator "\nVerbosity:\n\n"
37
+ opts.on("-q", "--quiet", "Run quietly, set logging to ERROR level [default INFO]") {options[:log_level] = 'error'}
38
+ opts.on("--logger filename",String,"Log to file [default #{options[:logger]}]") { |name| options[:logger] = name}
39
+ opts.on("--trace options",String,"Set log level [default INFO]. e.g. '--trace debug' to set logging level to DEBUG"){|s| options[:log_level] = s}
40
+ end; o.parse!
41
+ if ARGV.length != 0 or options[:trails_fasta_file].nil? or options[:kmer_multiple_abundance_file].nil? or options[:output_trail_kmer_coverage_file].nil?
42
+ $stderr.puts "Options not correctly specified. Found:"
43
+ pp options
44
+ $stderr.puts o
45
+ exit 1
46
+ end
47
+ # Setup logging
48
+ Bio::Log::CLI.logger(options[:logger]); Bio::Log::CLI.trace(options[:log_level]); log = Bio::Log::LoggerPlus.new(LOG_NAME); Bio::Log::CLI.configure(LOG_NAME)
49
+
50
+ # read in fasta file of trails
51
+ log.info "Reading trail sequences from #{options[:trails_fasta_file]}"
52
+ fasta_seqs = {}
53
+ Bio::FlatFile.foreach(options[:trails_fasta_file]) do |e|
54
+ name = e.definition
55
+ seq = e.seq.seq
56
+ fasta_seqs[name]=seq
57
+ end
58
+
59
+ log.info "Reading kmer abundances from #{options[:kmer_multiple_abundance_file]}.."
60
+ kmer_hash = Bio::KmerMultipleAbundanceHash.parse_from_file options[:kmer_multiple_abundance_file]
61
+ log.info "Finished reading the kmer abundances"
62
+
63
+ if options[:output_trail_kmer_coverage_file]
64
+ log.info "Writing out kmer coverages to #{options[:output_trail_kmer_coverage_file]}.."
65
+ writer = Bio::AssemblyGraphAlgorithms::KmerCoverageBasedPathFilter.new
66
+ io = File.open(options[:output_trail_kmer_coverage_file],'w')
67
+ fasta_seqs.each do |name, seq|
68
+ log.debug "Writing coverages for #{name}"
69
+ writer.write_depths(io, name, seq, kmer_hash)
70
+ end
71
+ log.info "Finished writing"
72
+ end
73
+
74
+ #log.info "Filtering trail(s) based on kmer coverage, requiring each kmer in the path to have a minimum of #{options[:kmer_path_filter_min_coverage]} coverage in patterned reads, except for the #{options[:kmer_path_end_exclusion_length]}bp at the ends"
75
+ #kmer_path_filter = Bio::AssemblyGraphAlgorithms::KmerCoverageBasedPathFilter.new
76
+ #thresholds = desired_pattern.collect{|c| c == true ? 1 : 0}
77
+ #log.info "Using thresholds for filtering: #{thresholds}"
78
+ #trails = kmer_path_filter.filter(trails, kmer_hash, thresholds, :exclude_ending_length => options[:kmer_path_end_exclusion_length])
79
+ #log.info "After filtering remained #{trails.length} trails"
80
+
81
+ #trails.each_with_index do |trail, i|
82
+ # puts ">trail#{i+1}"
83
+ # puts trail.sequence
84
+ #end
@@ -0,0 +1,56 @@
1
+ #(c) Copyright 2011 Raoul Bonnal. All Rights Reserved. Modified by Ben Woodcroft, 2014
2
+
3
+ # create Rakefile for shared library compilation
4
+
5
+
6
+
7
+ path = File.expand_path(File.dirname(__FILE__))
8
+
9
+ path_external = File.join(path, "../lib/external")
10
+
11
+ version = File.open(File.join(path_external,"VERSION"),'r')
12
+ Version = version.read
13
+ version.close
14
+
15
+ File.open(File.join(path,"Rakefile"),"w") do |rakefile|
16
+ rakefile.write <<-RAKE
17
+ require 'rbconfig'
18
+ require 'fileutils'
19
+ include FileUtils::Verbose
20
+ require 'rake/clean'
21
+
22
+ path = File.expand_path(File.dirname(__FILE__))
23
+ path_external = File.join(File.dirname(__FILE__), "../lib/external")
24
+
25
+ task :compile do
26
+ cd(File.join(File.dirname(__FILE__),'src')) do
27
+ #sh "patch -p1 < ../bioruby.patch"
28
+ case Config::CONFIG['host_os']
29
+ when /linux/
30
+
31
+ # Create library with default install params
32
+ $stdout.puts "Making velvet shared library with default parameters"
33
+ sh "make MAXKMERLENGTH=255 finishm velveth velvetg"
34
+ $stdout.puts "Finished make finishm"
35
+ shared_location = 'obj/shared'
36
+ cp(File.join(shared_location,"libfinishm.so.1.0"), path_external)
37
+
38
+ when /darwin/
39
+ raise NotImplementedError, "possibly will work, but bio-velvet_underground is not tested on OSX"
40
+ when /mswin|mingw/ then raise NotImplementedError, "bio-velvet_underground library is not available for Windows platform"
41
+ end #case
42
+ end #cd
43
+ end
44
+
45
+ task :clean do
46
+ cd(File.join(path,'src')) do
47
+ sh "make clean"
48
+ end
49
+ rm(File.join(path_external,"libvelvet.so.1.0"))
50
+ end
51
+
52
+ task :default => [:compile]
53
+
54
+ RAKE
55
+
56
+ end
@@ -0,0 +1,140 @@
1
+ CC = gcc
2
+ CFLAGS = -Wall
3
+ DEBUG = -g
4
+ LIBS = -lm
5
+ OPT = -O3
6
+ MAXKMERLENGTH=31
7
+ CATEGORIES=2
8
+ DEF = -D MAXKMERLENGTH=$(MAXKMERLENGTH) -D CATEGORIES=$(CATEGORIES)
9
+ PDFLATEX_VERSION := $(shell pdflatex --version 2> /dev/null)
10
+
11
+ # Mac OS users: uncomment the following lines
12
+ CFLAGS = -Wall -m64
13
+
14
+ # Sparc/Solaris users: uncomment the following line
15
+ # CFLAGS = -Wall -m64
16
+
17
+ ifdef BIGASSEMBLY
18
+ override DEF := $(DEF) -D BIGASSEMBLY
19
+ endif
20
+
21
+ ifdef VBIGASSEMBLY
22
+ override DEF := $(DEF) -D BIGASSEMBLY -D VBIGASSEMBLY
23
+ endif
24
+
25
+
26
+ ifdef LONGSEQUENCES
27
+ override DEF := $(DEF) -D LONGSEQUENCES
28
+ endif
29
+
30
+ # OpenMP
31
+ ifdef OPENMP
32
+ override CFLAGS := $(CFLAGS) -fopenmp
33
+ endif
34
+
35
+ # Per library coverage
36
+ ifdef SINGLE_COV_CAT
37
+ override DEF := $(DEF) -D SINGLE_COV_CAT
38
+ endif
39
+
40
+ OBJ = obj/tightString.o obj/run.o obj/splay.o obj/splayTable.o obj/graph.o obj/run2.o obj/fibHeap.o obj/fib.o obj/concatenatedGraph.o obj/passageMarker.o obj/graphStats.o obj/correctedGraph.o obj/dfib.o obj/dfibHeap.o obj/recycleBin.o obj/readSet.o obj/binarySequences.o obj/shortReadPairs.o obj/locallyCorrectedGraph.o obj/graphReConstruction.o obj/roadMap.o obj/preGraph.o obj/preGraphConstruction.o obj/concatenatedPreGraph.o obj/readCoherentGraph.o obj/utility.o obj/kmer.o obj/scaffold.o obj/kmerOccurenceTable.o obj/allocArray.o obj/autoOpen.o obj/probe_node_finder.o obj/readToNode.o obj/runReadToNode.o
41
+ OBJDBG = $(subst obj,obj/dbg,$(OBJ))
42
+ OBJSHARED = $(subst obj,obj/shared,$(OBJ))
43
+
44
+ default : cleanobj zlib obj velveth velvetg doc
45
+
46
+ clean : clean-zlib
47
+ -rm obj/*.o obj/dbg/*.o ./velvet* obj/shared/*.o
48
+ -rm -f doc/manual_src/Manual.toc doc/manual_src/Manual.aux doc/manual_src/Manual.out doc/manual_src/Manual.log
49
+ -rm -f doc/manual_src/Columbus_manual.aux doc/manual_src/Columbus_manual.out doc/manual_src/Columbus_manual.log
50
+
51
+ cleanobj:
52
+ # -rm obj/*.o obj/dbg/*.o obj/shared/*.o
53
+
54
+ ifdef BUNDLEDZLIB
55
+ Z_LIB_DIR=third-party/zlib-1.2.3
56
+ Z_LIB_FILES=$(Z_LIB_DIR)/*.o
57
+ override DEF := $(DEF) -D BUNDLEDZLIB
58
+
59
+ zlib:
60
+ cd $(Z_LIB_DIR); ./configure; make; rm minigzip.o; rm example.o
61
+
62
+ clean-zlib :
63
+ cd $(Z_LIB_DIR) && make clean
64
+ else
65
+ Z_LIB_FILES=-lz
66
+
67
+ zlib :
68
+ clean-zlib :
69
+ endif
70
+
71
+ velveth : obj
72
+ $(CC) $(CFLAGS) $(OPT) $(LDFLAGS) -o velveth obj/tightString.o obj/run.o obj/recycleBin.o obj/splay.o obj/splayTable.o obj/readSet.o obj/binarySequences.o obj/utility.o obj/kmer.o obj/kmerOccurenceTable.o obj/autoOpen.o $(Z_LIB_FILES) $(LIBS)
73
+
74
+
75
+ velvetg : obj
76
+ $(CC) $(CFLAGS) $(OPT) $(LDFLAGS) -o velvetg obj/tightString.o obj/graph.o obj/run2.o obj/fibHeap.o obj/fib.o obj/concatenatedGraph.o obj/passageMarker.o obj/graphStats.o obj/correctedGraph.o obj/dfib.o obj/dfibHeap.o obj/recycleBin.o obj/readSet.o obj/binarySequences.o obj/shortReadPairs.o obj/scaffold.o obj/locallyCorrectedGraph.o obj/graphReConstruction.o obj/roadMap.o obj/preGraph.o obj/preGraphConstruction.o obj/concatenatedPreGraph.o obj/readCoherentGraph.o obj/utility.o obj/kmer.o obj/kmerOccurenceTable.o obj/allocArray.o obj/autoOpen.o obj/readToNode.o $(Z_LIB_FILES) $(LIBS)
77
+
78
+ debug : override DEF := $(DEF) -D DEBUG
79
+ debug : cleanobj obj/dbg
80
+ $(CC) $(CFLAGS) $(LDFLAGS) $(DEBUG) -o velveth obj/dbg/tightString.o obj/dbg/run.o obj/dbg/recycleBin.o obj/dbg/splay.o obj/dbg/splayTable.o obj/dbg/readSet.o obj/dbg/binarySequences.o obj/dbg/utility.o obj/dbg/kmer.o obj/dbg/kmerOccurenceTable.o obj/dbg/allocArray.o obj/dbg/autoOpen.o $(Z_LIB_FILES) $(LIBS)
81
+ $(CC) $(CFLAGS) $(LDFLAGS) $(DEBUG) -o velvetg obj/dbg/tightString.o obj/dbg/graph.o obj/dbg/run2.o obj/dbg/fibHeap.o obj/dbg/fib.o obj/dbg/concatenatedGraph.o obj/dbg/passageMarker.o obj/dbg/graphStats.o obj/dbg/correctedGraph.o obj/dbg/dfib.o obj/dbg/dfibHeap.o obj/dbg/recycleBin.o obj/dbg/readSet.o obj/dbg/binarySequences.o obj/dbg/shortReadPairs.o obj/dbg/scaffold.o obj/dbg/locallyCorrectedGraph.o obj/dbg/graphReConstruction.o obj/dbg/roadMap.o obj/dbg/preGraph.o obj/dbg/preGraphConstruction.o obj/dbg/concatenatedPreGraph.o obj/dbg/readCoherentGraph.o obj/dbg/utility.o obj/dbg/kmer.o obj/dbg/kmerOccurenceTable.o obj/dbg/allocArray.o obj/dbg/autoOpen.o $(Z_LIB_FILES) $(LIBS)
82
+
83
+ color : override DEF := $(DEF) -D COLOR
84
+ color : cleanobj obj_de
85
+ $(CC) $(CFLAGS) $(OPT) $(LDFLAGS) -o velveth_de obj/tightString.o obj/run.o obj/recycleBin.o obj/splay.o obj/splayTable.o obj/readSet.o obj/binarySequences.o obj/utility.o obj/kmer.o obj/kmerOccurenceTable.o obj/allocArray.o obj/autoOpen.o $(Z_LIB_FILES) $(LIBS)
86
+ $(CC) $(CFLAGS) $(OPT) $(LDFLAGS) -o velvetg_de obj/tightString.o obj/graph.o obj/run2.o obj/fibHeap.o obj/fib.o obj/concatenatedGraph.o obj/passageMarker.o obj/graphStats.o obj/correctedGraph.o obj/dfib.o obj/dfibHeap.o obj/recycleBin.o obj/readSet.o obj/binarySequences.o obj/shortReadPairs.o obj/scaffold.o obj/locallyCorrectedGraph.o obj/graphReConstruction.o obj/roadMap.o obj/preGraph.o obj/preGraphConstruction.o obj/concatenatedPreGraph.o obj/readCoherentGraph.o obj/utility.o obj/kmer.o obj/kmerOccurenceTable.o obj/allocArray.o obj/autoOpen.o $(Z_LIB_FILES) $(LIBS)
87
+
88
+ colordebug : override DEF := $(DEF) -D COLOR -D DEBUG
89
+ colordebug : cleanobj obj/dbg_de
90
+ $(CC) $(CFLAGS) $(LDFLAGS) $(DEBUG) -o velveth_de obj/dbg/tightString.o obj/dbg/run.o obj/dbg/recycleBin.o obj/dbg/splay.o obj/dbg/splayTable.o obj/dbg/readSet.o obj/dbg/binarySequences.o obj/dbg/utility.o obj/dbg/kmer.o obj/dbg/kmerOccurenceTable.o obj/dbg/allocArray.o obj/dbg/autoOpen.o $(Z_LIB_FILES) $(LIBS)
91
+ $(CC) $(CFLAGS) $(LDFLAGS) $(DEBUG) -o velvetg_de obj/dbg/tightString.o obj/dbg/graph.o obj/dbg/run2.o obj/dbg/fibHeap.o obj/dbg/fib.o obj/dbg/concatenatedGraph.o obj/dbg/passageMarker.o obj/dbg/graphStats.o obj/dbg/correctedGraph.o obj/dbg/dfib.o obj/dbg/dfibHeap.o obj/dbg/recycleBin.o obj/dbg/readSet.o obj/dbg/binarySequences.o obj/dbg/shortReadPairs.o obj/dbg/scaffold.o obj/dbg/locallyCorrectedGraph.o obj/dbg/graphReConstruction.o obj/dbg/roadMap.o obj/dbg/preGraph.o obj/dbg/preGraphConstruction.o obj/dbg/concatenatedPreGraph.o obj/dbg/readCoherentGraph.o obj/dbg/utility.o obj/dbg/kmer.o obj/dbg/kmerOccurenceTable.o obj/dbg/allocArray.o obj/dbg/autoOpen.o $(Z_LIB_FILES) $(LIBS)
92
+
93
+ objdir:
94
+ mkdir -p obj
95
+
96
+ obj: zlib cleanobj objdir $(OBJ)
97
+
98
+ obj_de: override DEF := $(DEF) -D COLOR
99
+ obj_de: zlib cleanobj objdir $(OBJ)
100
+
101
+ obj/dbgdir:
102
+ mkdir -p obj/dbg
103
+
104
+ obj/dbg: override DEF := $(DEF) -D DEBUG
105
+ obj/dbg: zlib cleanobj obj/dbgdir $(OBJDBG)
106
+
107
+ obj/dbg_de: override DEF := $(DEF) -D COLOR -D DEBUG
108
+ obj/dbg_de: zlib cleanobj obj/dbgdir $(OBJDBG)
109
+
110
+ obj/%.o: src/%.c
111
+ $(CC) $(CFLAGS) $(OPT) $(DEF) -c $? -o $@
112
+
113
+ obj/dbg/%.o: src/%.c
114
+ $(CC) $(CFLAGS) $(DEBUG) $(DEF) -c $? -o $@
115
+
116
+ doc: Manual.pdf
117
+
118
+ Manual.pdf: doc/manual_src/Manual.tex doc/manual_src/Columbus_manual.tex
119
+ ifdef PDFLATEX_VERSION
120
+ cd doc/manual_src; pdflatex Manual.tex; pdflatex Manual.tex; mv Manual.pdf ../..; pdflatex Columbus_manual.tex; mv Columbus_manual.pdf ../..
121
+ else
122
+ @echo pdflatex not found, cannot rebuild the manual.
123
+ endif
124
+
125
+ test: velvetg velveth
126
+ cd tests && ./run-tests.sh
127
+
128
+ sharedobjdir:
129
+ mkdir -p obj/shared
130
+
131
+ obj/shared: sharedobjdir $(OBJSHARED)
132
+
133
+ obj/shared/%.o: src/%.c
134
+ $(CC) -fPIC $(CFLAGS) $(DEBUG) $(DEF) -c $? -o $@
135
+
136
+ finishm: zlib obj/shared
137
+ cd obj/shared && gcc -shared -Wl,-soname,libfinishm.so.1 -o libfinishm.so.1.0 allocArray.o autoOpen.o binarySequences.o concatenatedGraph.o concatenatedPreGraph.o correctedGraph.o dfibHeap.o dfib.o fibHeap.o fib.o graph.o graphReConstruction.o graphStats.o kmer.o kmerOccurenceTable.o locallyCorrectedGraph.o passageMarker.o preGraphConstruction.o preGraph.o readCoherentGraph.o readSet.o recycleBin.o roadMap.o scaffold.o shortReadPairs.o splay.o splayTable.o tightString.o utility.o probe_node_finder.o readToNode.o
138
+
139
+ readToNodeBinary: obj
140
+ $(CC) $(CFLAGS) $(OPT) $(LDFLAGS) -o readToNode obj/runReadToNode.o obj/tightString.o obj/graph.o obj/fibHeap.o obj/fib.o obj/concatenatedGraph.o obj/passageMarker.o obj/graphStats.o obj/correctedGraph.o obj/dfib.o obj/dfibHeap.o obj/recycleBin.o obj/readSet.o obj/binarySequences.o obj/shortReadPairs.o obj/scaffold.o obj/locallyCorrectedGraph.o obj/graphReConstruction.o obj/roadMap.o obj/preGraph.o obj/preGraphConstruction.o obj/concatenatedPreGraph.o obj/readCoherentGraph.o obj/utility.o obj/kmer.o obj/kmerOccurenceTable.o obj/allocArray.o obj/autoOpen.o obj/readToNode.o $(Z_LIB_FILES) $(LIBS)
@@ -0,0 +1,305 @@
1
+ /*
2
+ Copyright 2009 Sylvain Foret (sylvain.foret@anu.edu.au)
3
+
4
+ This file is part of Velvet.
5
+
6
+ Velvet is free software; you can redistribute it and/or modify
7
+ it under the terms of the GNU General Public License as published by
8
+ the Free Software Foundation; either version 2 of the License, or
9
+ (at your option) any later version.
10
+
11
+ Velvet is distributed in the hope that it will be useful,
12
+ but WITHOUT ANY WARRANTY; without even the implied warranty of
13
+ MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
14
+ GNU General Public License for more details.
15
+
16
+ You should have received a copy of the GNU General Public License
17
+ along with Velvet; if not, write to the Free Software
18
+ Foundation, Inc., 51 Franklin St, Fifth Floor, Boston, MA 02110-1301 USA
19
+
20
+ */
21
+
22
+ #include <stdlib.h>
23
+ #include <stdio.h>
24
+ #include <unistd.h>
25
+ #ifdef _OPENMP
26
+ #include <omp.h>
27
+ #endif
28
+
29
+ #include "allocArray.h"
30
+ #include "utility.h"
31
+
32
+ #ifdef DEBUG
33
+ #define NB_PAGES_ALLOC 1
34
+ #define BLOCKS_ALLOC_SIZE 1
35
+ #else
36
+ #define NB_PAGES_ALLOC 128
37
+ #define BLOCKS_ALLOC_SIZE 128
38
+ #endif
39
+
40
+ #ifndef VBIGASSEMBLY
41
+ #define INDEX_LENGTH 32
42
+ #else
43
+ #define INDEX_LENGTH 64
44
+ #endif
45
+
46
+ struct AllocArrayFreeElement_st
47
+ {
48
+ AllocArrayFreeElement *next;
49
+ ArrayIdx idx;
50
+ };
51
+
52
+ static void initAllocArray(AllocArray * array, size_t elementSize, char * name)
53
+ {
54
+ array->freeElements = NULL;
55
+ array->elementSize = elementSize;
56
+ array->blockSize = sysconf (_SC_PAGESIZE) * NB_PAGES_ALLOC;
57
+ array->maxElements = array->blockSize / array->elementSize;
58
+ array->maxBlocks = BLOCKS_ALLOC_SIZE;
59
+ array->blocks = mallocOrExit (array->maxBlocks, void*);
60
+ array->blocks[0] = mallocOrExit (array->blockSize, char);
61
+ array->currentBlocks = 1;
62
+ array->currentElements = 0;
63
+ #ifdef DEBUG
64
+ array->elementsRecycled = 0;
65
+ array->elementsAllocated = 0;
66
+ array->name = name;
67
+ #endif
68
+ #ifdef _OPENMP
69
+ array->nbThreads = 0;
70
+ #endif
71
+ }
72
+
73
+ AllocArray*
74
+ newAllocArray (size_t elementSize, char *name)
75
+ {
76
+ AllocArray *array;
77
+
78
+ if (elementSize < sizeof(AllocArrayFreeElement)) {
79
+ velvetLog("Elements too small to create an AllocArray!\n");
80
+ exit(-1);
81
+ }
82
+ array = mallocOrExit (1, AllocArray);
83
+ initAllocArray(array, elementSize, name);
84
+ return array;
85
+ }
86
+
87
+ void
88
+ destroyAllocArrayChunks (AllocArray *array)
89
+ {
90
+ size_t i;
91
+ for (i = 0; i < array->currentBlocks; i++)
92
+ free (array->blocks[i]);
93
+ free (array->blocks);
94
+ }
95
+
96
+ void
97
+ destroyAllocArray (AllocArray *array)
98
+ {
99
+ if (array)
100
+ {
101
+ destroyAllocArrayChunks(array);
102
+ #ifdef DEBUG
103
+ velvetLog(">>> Allocation summary for %s\n", array->name);
104
+ velvetLog(">>> Alloc'ed %ld bytes\n", array->blockSize * array->currentBlocks);
105
+ velvetLog(">>> Alloc'ed %ld elements\n", array->elementsAllocated);
106
+ velvetLog(">>> Recycled %ld elements\n", array->elementsRecycled);
107
+ #endif
108
+ free (array);
109
+ }
110
+ }
111
+
112
+ ArrayIdx
113
+ allocArrayAllocate (AllocArray *array)
114
+ {
115
+ if (array->freeElements != NULL)
116
+ {
117
+ AllocArrayFreeElement *element;
118
+
119
+ element = array->freeElements;
120
+ array->freeElements = element->next;
121
+ #ifdef DEBUG
122
+ array->elementsRecycled++;
123
+ #endif
124
+ return element->idx;
125
+ }
126
+ if (array->currentElements >= array->maxElements)
127
+ {
128
+ if (array->currentBlocks == array->maxBlocks)
129
+ {
130
+ array->maxBlocks += BLOCKS_ALLOC_SIZE;
131
+ array->blocks = reallocOrExit (array->blocks, array->maxBlocks, void*);
132
+ }
133
+ array->blocks[array->currentBlocks] = mallocOrExit (array->blockSize, char);
134
+ array->currentBlocks++;
135
+ array->currentElements = 0;
136
+ }
137
+ array->currentElements++;
138
+ #ifndef VBIGASSEMBLY
139
+ if (array->maxElements * (array->currentBlocks - 1) + array->currentElements == UINT32_MAX)
140
+ {
141
+ #ifdef DEBUG
142
+ velvetLog (">>> Reached maximum `%s' addressable with %i bits\n", array->name, INDEX_LENGTH);
143
+ #else
144
+ velvetLog (">>> Reached maximum elements addressable with %i bits\n", INDEX_LENGTH);
145
+ #endif
146
+ abort();
147
+ }
148
+ #endif
149
+
150
+ #ifdef DEBUG
151
+ array->elementsAllocated++;
152
+ #endif
153
+
154
+ return array->maxElements * (array->currentBlocks - 1) + array->currentElements;
155
+ }
156
+
157
+ static void*
158
+ allocArrayGetElement (AllocArray *array, ArrayIdx idx)
159
+ {
160
+ if (idx != NULL_IDX)
161
+ {
162
+ const ArrayIdx i = idx - 1;
163
+ const ArrayIdx blockIdx = i / array->maxElements;
164
+ const ArrayIdx elementIdx = i % array->maxElements;
165
+ return ((char*)(array->blocks[blockIdx])) + elementIdx * array->elementSize;
166
+ }
167
+ return NULL;
168
+ }
169
+
170
+ void
171
+ allocArrayFree (AllocArray *array, ArrayIdx idx)
172
+ {
173
+ if (idx != NULL_IDX)
174
+ {
175
+ AllocArrayFreeElement *freeElem;
176
+
177
+ freeElem = allocArrayGetElement (array, idx);
178
+ freeElem->idx = idx;
179
+ freeElem->next = array->freeElements;
180
+ array->freeElements = freeElem;
181
+ }
182
+ }
183
+
184
+ #ifdef _OPENMP
185
+
186
+ #define BLOCKS_ALLOC_SHIFT 16
187
+
188
+ static void initAllocArrayArray(AllocArray *array,
189
+ void *blocks,
190
+ size_t nbBlocks,
191
+ size_t elementSize,
192
+ char *name,
193
+ int nbThreads,
194
+ int thisThread)
195
+ {
196
+ array->freeElements = NULL;
197
+ array->elementSize = elementSize;
198
+ array->blockSize = (((size_t) 1) << BLOCKS_ALLOC_SHIFT) * elementSize;
199
+ array->maxElements = (((size_t) 1) << BLOCKS_ALLOC_SHIFT);
200
+ array->maxBlocks = nbBlocks;
201
+ array->blocks = blocks;
202
+ array->blocks[thisThread] = callocOrExit(array->blockSize, char);
203
+ array->currentBlocks = thisThread;
204
+ array->currentElements = 0;
205
+ array->nbThreads = nbThreads;
206
+ #ifdef DEBUG
207
+ array->elementsRecycled = 0;
208
+ array->elementsAllocated = 0;
209
+ array->name = name;
210
+ #endif
211
+ }
212
+
213
+ AllocArray *newAllocArrayArray(unsigned int n,
214
+ size_t elementSize,
215
+ char * name)
216
+ {
217
+ AllocArray *allocArray;
218
+ void **blocks;
219
+ size_t nbBlocks;
220
+ int i;
221
+
222
+ allocArray = callocOrExit (n + 1, AllocArray);
223
+ nbBlocks = (((size_t) 1) << (INDEX_LENGTH - BLOCKS_ALLOC_SHIFT));
224
+ blocks = callocOrExit(nbBlocks, void*);
225
+ for (i = 0; i < n; i++)
226
+ initAllocArrayArray(allocArray + i,
227
+ blocks,
228
+ nbBlocks,
229
+ elementSize,
230
+ name,
231
+ n, i);
232
+ /* Last element marker */
233
+ allocArray[n].currentBlocks = n;
234
+
235
+ return allocArray;
236
+ }
237
+
238
+ void destroyAllocArrayArray(AllocArray *allocArray)
239
+ {
240
+ int i;
241
+
242
+ for (i = 0; i < allocArray[0].maxBlocks; i++)
243
+ if (allocArray[0].blocks[i] != NULL)
244
+ free(allocArray[0].blocks[i]);
245
+
246
+ free(allocArray[0].blocks);
247
+ free(allocArray);
248
+ }
249
+
250
+ ArrayIdx allocArrayArrayAllocate(AllocArray *array)
251
+ {
252
+ int thread = omp_get_thread_num();
253
+
254
+ AllocArray * lastArray = array + array->nbThreads;
255
+ array += thread;
256
+ if (array->freeElements != NULL)
257
+ {
258
+ AllocArrayFreeElement *element;
259
+
260
+ element = array->freeElements;
261
+ array->freeElements = element->next;
262
+ #ifdef DEBUG
263
+ array->elementsRecycled++;
264
+ #endif
265
+ return element->idx;
266
+ }
267
+ if (array->currentElements >= array->maxElements)
268
+ {
269
+ #pragma omp critical
270
+ array->currentBlocks = lastArray->currentBlocks++;
271
+
272
+ if (array->currentBlocks >= array->maxBlocks)
273
+ {
274
+ #ifdef DEBUG
275
+ velvetLog(">>> Reached maximum `%s' addressable with %i bits\n",
276
+ array->name, INDEX_LENGTH);
277
+ #else
278
+ velvetLog(">>> Reached maximum elements addressable with %i bits\n", INDEX_LENGTH);
279
+ #endif
280
+ abort();
281
+ }
282
+ array->blocks[array->currentBlocks] = callocOrExit(array->blockSize, char);
283
+ array->currentElements = 0;
284
+ }
285
+ array->currentElements++;
286
+ #ifdef DEBUG
287
+ array->elementsAllocated++;
288
+ #endif
289
+ return array->maxElements * array->currentBlocks + array->currentElements;
290
+ }
291
+
292
+ void
293
+ allocArrayArrayFree(AllocArray *array, ArrayIdx idx)
294
+ {
295
+ if (idx != NULL_IDX)
296
+ {
297
+ AllocArrayFreeElement *freeElem;
298
+
299
+ freeElem = allocArrayGetElement(array, idx);
300
+ freeElem->idx = idx;
301
+ freeElem->next = array->freeElements;
302
+ array->freeElements = freeElem;
303
+ }
304
+ }
305
+ #endif