biotite 1.5.0__cp314-cp314-macosx_10_13_x86_64.whl

biotite/sequence/phylo/tree.pyx

@@ -0,0 +1,1169 @@
+# This source code is part of the Biotite package and is distributed
+# under the 3-Clause BSD License. Please see 'LICENSE.rst' for further
+# information.
+
+__name__ = "biotite.sequence.phylo"
+__author__ = "Patrick Kunzmann, Tom David Müller"
+__all__ = ["Tree", "TreeNode", "as_binary", "TreeError"]
+
+cimport cython
+cimport numpy as np
+
+import copy
+import numpy as np
+import networkx as nx
+from ...file import InvalidFileError
+from ...copyable import Copyable
+
+
+class Tree(Copyable):
+    """
+    __init__(root)
+    
+    A :class:`Tree` represents a rooted tree
+    (e.g. alignment guide tree or phylogenetic tree).
+
+    The tree itself wraps a *root* :class:`TreeNode` object,
+    accessible via the :attr:`root` property.
+
+    A :class:`Tree` is not a container itself:
+    Objects, e.g species names or sequences, that are represented by the
+    nodes, cannot be stored directly in a :class:`Tree` or its nodes.
+    Instead, each leaf :class:`TreeNode` has a reference index:
+    These indices refer to a separate list or array, containing the
+    actual reference objects.
+
+    The property :attr:`leaves` contains a list of the leaf nodes,
+    where the index of the leaf node in this list is equal to the
+    reference index of the leaf node (``leaf.index``).
+
+    The amount of leaves in a tree can be determined via the
+    :func:`len()` function.
+
+    Objects of this class are immutable.
+
+    Parameters
+    ----------
+    root: TreeNode
+        The root of the tree.
+        The constructor calls the node's :func:`as_root()` method,
+        in order to make it immutable.
+    
+    Attributes
+    ----------
+    root : TreeNode
+        The root node of the tree.
+    leaves : list of TreeNode
+        The leaf nodes of the tree.
+        The index of the leaf node in this list is equal to the
+        reference index of the leaf node.
+        This attribute is a shallow copy of the repsective internal
+        object.
+
+    Examples
+    --------
+
+    >>> objects = ["An object", "Another object", "Yet another one"]
+    >>> leaf1 = TreeNode(index=0)
+    >>> leaf2 = TreeNode(index=1)
+    >>> leaf3 = TreeNode(index=2)
+    >>> inter = TreeNode([leaf1, leaf2], [5.0, 7.0])
+    >>> root  = TreeNode([inter, leaf3], [3.0, 10.0])
+    >>> tree  = Tree(root)
+    >>> print(tree)
+    ((0:5.0,1:7.0):3.0,2:10.0):0.0;
+    >>> print([objects[node.index] for node in tree.leaves])
+    ['An object', 'Another object', 'Yet another one']
+    """
+    
+    def __init__(self, TreeNode root not None):
+        root.as_root()
+        self._root = root
+        
+        cdef list leaves_unsorted = self._root.get_leaves()
+        cdef int leaf_count = len(leaves_unsorted)
+        cdef np.ndarray indices = np.array(
+            [leaf.index for leaf in leaves_unsorted]
+        )
+        self._leaves = [None] * leaf_count
+        cdef int i
+        cdef int index
+        for i in range(len(indices)):
+            index = indices[i]
+            if index >= leaf_count or index < 0:
+                raise TreeError("The tree's indices are out of range")
+            self._leaves[index] = leaves_unsorted[i]
+    
+    def __copy_create__(self):
+        return Tree(self._root.copy())
+    
+    @property
+    def root(self):
+        return self._root
+    
+    @property
+    def leaves(self):
+        return copy.copy(self._leaves)
+    
+    def as_graph(self):
+        """
+        as_graph()
+        
+        Obtain a graph representation of the :class:`Tree`.
+
+        Returns
+        -------
+        bond_set : DiGraph
+            A *NetworkX* directed graph.
+            For a leaf node the graph node is its reference index.
+            For an intermediate and root node the graph node is a tuple
+            containing it children nodes.
+            Each edge has a ``"distance"`` attribute depicting the
+            distance between the nodes.
+            Each edge starts from the parent ends at its child.
+        
+        Examples
+        --------
+
+        >>> leaves = [TreeNode(index=i) for i in range(3)]
+        >>> intermediate = TreeNode([leaves[0], leaves[1]], [2.0, 3.0])
+        >>> root = TreeNode([intermediate, leaves[2]], [1.0, 5.0])
+        >>> tree = Tree(root)
+        >>> graph = tree.as_graph()
+        >>> for node_i, node_j in graph.edges:
+        ...     print(f"{str(node_i):12}  ->  {str(node_j):12}")
+        (0, 1)        ->  0
+        (0, 1)        ->  1
+        ((0, 1), 2)   ->  (0, 1)
+        ((0, 1), 2)   ->  2
+        """
+        cdef tuple children
+        cdef bint children_already_handled
+        cdef TreeNode node, child, parent
+
+        graph = nx.DiGraph()
+        
+        # This dict maps a TreeNode to its corresponding int or tuple
+        cdef dict node_repr = {}
+
+        # A First-In-First-Out queue for iterative handling of each node
+        # Starting with all leaf nodes 
+        cdef list queue = copy.copy(self._leaves)
+        # A set representation of the same queue for efficient
+        # '__contains__()' operation
+        cdef set queue_set = set(self._leaves)
+        while len(queue) > 0:
+            node = queue.pop(0)
+            
+            if node.is_leaf():
+                node_repr[node] = node.index
+            else:
+                children = node.children
+                children_handled = True
+                for child in children:
+                    if child not in node_repr:
+                        children_handled = False
+                # If the node representation of any child of this node
+                # is not calculated yet, put this node to the end of the
+                # queue and handle it later
+                if not children_handled:
+                    queue.append(node)
+                    continue
+                else:
+                    repr = tuple(node_repr[child] for child in children)
+                    node_repr[node] = repr
+                    # Add adges to children in graph
+                    for child in children:
+                        graph.add_edge(
+                            repr, node_repr[child], distance=child.distance
+                        )
+            
+            # This leads finally to termination of the loop:
+            # When the root node is handled the last element in the
+            # queue is handled and no new node is added to the queue
+            if not node.is_root():
+                parent = node.parent
+                # The parent node might be already in the queue from
+                # handling another child node
+                if parent not in queue_set:
+                    queue.append(parent)
+                    queue_set.add(parent)
+            
+            # Node is handled
+            # -> not in 'queue' anymore
+            # -> remove also from 'queue_set'
+            queue_set.remove(node)
+        
+        return graph
+
+        
+
+    def get_distance(self, index1, index2, bint topological=False):
+        """
+        get_distance(index1, index2, topological=False)
+        
+        Get the distance between two leaf nodes.
+
+        The distance is the sum of all distances from the each of the
+        two nodes to their lowest common ancestor.
+
+        Parameters
+        ----------
+        index1, index2 : int
+            The reference indices of the two leaf nodes, to calculate
+            the distance for.
+        topological : bool, optional
+            If True the topological distance is measured, i.e. all
+            child-parent distance is 1.
+            Otherwise, the distances from the `distance` attribute are
+            used.
+
+        Returns
+        -------
+        distance : float
+            The distance between the nodes.
+        
+        Examples
+        --------
+
+        >>> leaf1 = TreeNode(index=0)
+        >>> leaf2 = TreeNode(index=1)
+        >>> leaf3 = TreeNode(index=2)
+        >>> inter = TreeNode([leaf1, leaf2], [5.0, 7.0])
+        >>> root  = TreeNode([inter, leaf3], [3.0, 10.0])
+        >>> tree = Tree(root)
+        >>> print(tree.get_distance(0,1))
+        12.0
+        >>> print(tree.get_distance(0,2))
+        18.0
+        >>> print(tree.get_distance(1,2))
+        20.0
+        """
+        return self._leaves[index1].distance_to(
+            self._leaves[index2], topological
+        )
+    
+    def to_newick(self, labels=None, bint include_distance=True, 
+                  round_distance=None):
+        """
+        to_newick(labels=None, include_distance=True)
+
+        Obtain the Newick notation of the tree.
+
+        Parameters
+        ----------
+        labels : iterable object of str
+            The labels the indices in the leaf nodes srefer to
+        include_distance : bool
+            If true, the distances are displayed in the newick notation,
+            otherwise they are omitted.
+        round_distance : int, optional
+            If set, the distances are rounded to the given number of
+            digits.
+        
+        Returns
+        -------
+        newick : str
+            The Newick notation of the tree.
+
+        Examples
+        --------
+        
+        >>> leaf1 = TreeNode(index=0)
+        >>> leaf2 = TreeNode(index=1)
+        >>> leaf3 = TreeNode(index=2)
+        >>> inter = TreeNode([leaf1, leaf2], [5.0, 7.0])
+        >>> root  = TreeNode([inter, leaf3], [3.0, 10.0])
+        >>> tree = Tree(root)
+        >>> print(tree.to_newick())
+        ((0:5.0,1:7.0):3.0,2:10.0):0.0;
+        >>> print(tree.to_newick(include_distance=False))
+        ((0,1),2);
+        >>> labels = ["foo", "bar", "foobar"]
+        >>> print(tree.to_newick(labels=labels, include_distance=False))
+        ((foo,bar),foobar);
+        """
+        return self._root.to_newick(
+            labels, include_distance, round_distance
+        ) + ";"
+    
+    @staticmethod
+    def from_newick(str newick, list labels=None):
+        """
+        from_newick(newick, labels=None)
+        
+        Create a tree from a Newick notation.
+
+        Parameters
+        ----------
+        newick : str
+            The Newick notation to create the tree from.
+        labels : list of str, optional
+            If the Newick notation contains labels, that are not
+            parseable into reference indices,
+            i.e. they are not integers, this parameter can be provided
+            to convert these labels into reference indices.
+            The corresponding index is the position of the label in the
+            provided list.
+
+        Returns
+        -------
+        tree : Tree
+            A tree created from the Newick notation
+        
+        Notes
+        -----
+        This function does accept but does not require the Newick string
+        to have the terminal semicolon.
+        
+        Keep in mind that the :class:`Tree` class does not support any
+        labels on intermediate nodes.
+        If the string contains such labels, they are discarded.
+        """
+        newick = newick.strip()
+        if len(newick) == 0:
+            raise InvalidFileError("Newick string is empty")
+        # Remove terminal colon as required by 'TreeNode.from_newick()'
+        if newick[-1] == ";":
+            newick = newick[:-1]
+        root, distance = TreeNode.from_newick(newick, labels)
+        return Tree(root)
+
+    def __str__(self):
+        return self.to_newick()
+    
+    def __len__(self):
+        return len(self._leaves)
+    
+    def __eq__(self, item):
+        if not isinstance(item, Tree):
+            return False
+        return self._root == item._root
+    
+    def __hash__(self):
+        return hash(self._root)
+
+
+cdef class TreeNode:
+    """
+    __init__(children=None, distances=None, index=None)
+    
+    :class:`TreeNode` objects are part of a rooted tree
+    (e.g. alignment guide tree).
+    There are two :class:`TreeNode` subtypes:
+        
+        - Leaf node - Cannot have child nodes but has an index referring
+          to an array-like reference object.
+        - Intermediate node - Has child nodes but no reference index
+    
+    This subtype is determined based on whether child nodes were given
+    to the constructor.
+    
+    Every :class:`TreeNode` has a reference to its parent node.
+    A root node is node without a parent node, that is finalized
+    using `as_root()`.
+    The call of this function prevents that a the node can be used as
+    child.
+
+    :class:`TreeNode` objects are semi-immutable:
+    The child nodes or the reference index are fixed at the time of
+    creation.
+    Only the parent can be set once, when the parent node is created. 
+    :class:`TreeNode` objects that are finalized using `as_root()` are
+    completely immutable.
+
+    All object properties are read-only.
+
+    Parameters
+    ----------
+    children: array-like object of TreeNode, length=n, optional
+        The children of this node.
+        As this causes the creation of an intermediate node,
+        this parameter cannot be used in combination with `index`.
+    distances: array-like object of float, length=n, optional
+        The distances of the child nodes to this node.
+        Must be set if `children` is set.
+    index: int, optional
+        Index to a reference array-like object
+        (e.g. list of sequences or labels).
+        Must be a positive integer.
+        As this causes the creation of a leaf node, this parameter
+        cannot be used in combination with the other parameters.
+    
+    Attributes
+    ----------
+    parent : TreeNode
+        The parent node.
+        `None` if node has no parent.
+    children : tuple of TreeNode
+        The child nodes.
+        `None` if node is a leaf node.
+    index : int
+        The index to a reference array-like object.
+        `None` if node is not a leaf node.
+    distance : float
+        Distance to parent node.
+        `None` if `parent` is `Ǹone`.
+
+    Examples
+    --------
+    Creating leaf nodes:
+    
+    >>> leaf1 = TreeNode(index=0)
+    >>> leaf2 = TreeNode(index=1)
+    >>> leaf3 = TreeNode(index=2)
+
+    Creating intermediate nodes as parent of those leaf nodes:
+
+    >>> inter = TreeNode([leaf1, leaf2], [5.0, 7.0])
+    >>> root  = TreeNode([inter, leaf3], [3.0, 10.0])
+    >>> print(root)
+    ((0:5.0,1:7.0):3.0,2:10.0):0.0
+    """
+
+    cdef int _index
+    cdef float _distance
+    cdef bint _is_root
+    cdef TreeNode _parent
+    cdef tuple _children
+
+    def __cinit__(self, children=None, distances=None, index=None):
+        self._is_root = False
+        self._distance = 0
+        self._parent = None
+        cdef TreeNode child
+        cdef float distance
+        if index is None:
+            # Node is intermediate -> has children
+            if children is None or distances is None:
+                raise TypeError(
+                    "Either reference index (for terminal node) or "
+                    "child nodes including the distance "
+                    "(for intermediate node) must be set"
+                )
+            for item in children:
+                if not isinstance(item, TreeNode):
+                    raise TypeError(
+                        f"Expected 'TreeNode', but got '{type(item).__name__}'"
+                    )
+            for item in distances:
+                if not isinstance(item, float) and not isinstance(item, int):
+                    raise TypeError(
+                        f"Expected 'float' or 'int', "
+                        f"but got '{type(item).__name__}'"
+                    )
+            if len(children) == 0:
+                raise TreeError(
+                    "Intermediate nodes must at least contain one child node"
+                )
+            if len(children) != len(distances):
+                raise ValueError(
+                    "The number of children must equal the number of distances"
+                )
+            for i in range(len(children)):
+                for j in range(len(children)):
+                    if i != j and children[i] is children[j]:
+                        raise TreeError(
+                            "Two child nodes cannot be the same object"
+                        )
+            self._index = -1
+            self._children = tuple(children)
+            for child, distance in zip(children, distances):
+                child._set_parent(self, distance)
+        elif index < 0:
+            raise ValueError("Index cannot be negative")
+        else:
+            # Node is terminal -> has no children
+            if children is not None or distances is not None:
+                raise TypeError(
+                    "Reference index and child nodes are mutually exclusive"
+                )
+            self._index = index
+            self._children = None
+    
+    def _set_parent(self, TreeNode parent not None, float distance):
+        if self._parent is not None or self._is_root:
+            raise TreeError("Node already has a parent")
+        self._parent = parent
+        self._distance = distance
+    
+    def copy(self):
+        """
+        copy()
+
+        Create a deep copy of this :class:`TreeNode`.
+
+        The copy includes this node, its reference index and deep copies
+        of its child nodes.
+        The parent node and the distance to it is not included.
+        """
+        if self.is_leaf():
+            return TreeNode(index=self._index)
+        else:
+            distances = [child.distance for child in self._children]
+            children_clones = [child.copy() for child in self._children]
+            return TreeNode(children_clones, distances)
+
+    @property
+    def index(self):
+        return None if self._index == -1 else self._index
+    
+    @property
+    def children(self):
+        return self._children
+    
+    @property
+    def parent(self):
+        return self._parent
+    
+    @property
+    def distance(self):
+        return None if self._parent is None else self._distance
+
+    def is_leaf(self):
+        """
+        is_leaf()
+        
+        Check if the node is a leaf node.
+
+        Returns
+        -------
+        is_leaf : bool
+            True if the node is a leaf node, false otherwise.
+        """
+        return False if self._index == -1 else True
+    
+    def is_root(self):
+        """
+        is_root()
+        
+        Check if the node is a root node.
+
+        Returns
+        -------
+        is_root : bool
+            True if the node is a root node, false otherwise.
+        """
+        return bool(self._is_root)
+    
+    def as_root(self):
+        """
+        as_root()
+        
+        Convert the node into a root node.
+
+        When a root node is used as `child` parameter in the
+        construction of a potential parent node, a :class:`TreeError` is
+        raised.
+        """
+        if self._parent is not None:
+            raise TreeError("Node has parent, cannot be a root node")
+        self._is_root = True
+    
+    def distance_to(self, TreeNode node, bint topological=False):
+        """
+        distance_to(node, topological=False)
+        
+        Get the distance of this node to another node.
+
+        The distance is the sum of all distances from this and the other
+        node to the lowest common ancestor.
+
+        Parameters
+        ----------
+        node : TreeNode
+            The second node for distance calculation.
+        
+
+        Returns
+        -------
+        distance : float
+            The distance of this node to `node`.
+        
+        Raises
+        ------
+        TreeError
+            If the nodes have no common ancestor.
+        
+        Examples
+        --------
+
+        >>> leaf1 = TreeNode(index=0)
+        >>> leaf2 = TreeNode(index=1)
+        >>> leaf3 = TreeNode(index=2)
+        >>> inter = TreeNode([leaf1, leaf2], [5.0, 7.0])
+        >>> root  = TreeNode([inter, leaf3], [3.0, 10.0])
+        >>> print(leaf1.distance_to(leaf2))
+        12.0
+        >>> print(leaf1.distance_to(leaf3))
+        18.0
+        """
+        # Sum distances until LCA has been reached
+        cdef float distance = 0
+        cdef TreeNode current_node = None
+        cdef TreeNode lca = self.lowest_common_ancestor(node)
+        if lca is None:
+            raise TreeError("The nodes do not have a common ancestor")
+        current_node = self
+        while current_node is not lca:
+            if topological:
+                distance += 1
+            else:
+                distance += current_node._distance
+            current_node = current_node._parent
+        current_node = node
+        while current_node is not lca:
+            if topological:
+                distance += 1
+            else:
+                distance += current_node._distance
+            current_node = current_node._parent
+        return distance
+    
+    def lowest_common_ancestor(self, TreeNode node):
+        """
+        lowest_common_ancestor(node)
+        
+        Get the lowest common ancestor of this node and another node.
+
+        Parameters
+        ----------
+        node : TreeNode
+            The node to get the lowest common ancestor with.
+
+        Returns
+        -------
+        ancestor : TreeNode or None
+            The lowest common ancestor. `None` if the nodes have no
+            common ancestor, i.e. they are not in the same tree
+        """
+        cdef int i
+        cdef TreeNode lca = None
+        # Create two paths from the leaves to root
+        cdef list self_path = _create_path_to_root(self)
+        cdef list other_path = _create_path_to_root(node)
+        # Reverse Iteration through path (beginning from root)
+        # until the paths diverge
+        for i in range(-1, -min(len(self_path), len(other_path))-1, -1):
+            if self_path[i] is other_path[i]:
+                # Same node -> common ancestor
+                lca = self_path[i]
+            else:
+                # Different node -> Not common ancestor
+                # -> return last common ancewstor found
+                break
+        return lca
+    
+    def get_indices(self):
+        """
+        get_indices()
+        
+        Get an array of reference indices that leaf nodes of this node
+        contain.
+
+        This method identifies all leaf nodes, which have this node as
+        ancestor and puts the contained indices into an array.
+        If this node is a leaf node itself, the array contains the
+        reference index of this node as single element.
+
+        Returns
+        -------
+        indices : ndarray, dtype=int32
+            The reference indices of direct and indirect child leaf
+            nodes of this node.
+
+        Examples
+        --------
+
+        >>> leaf0 = TreeNode(index=0)
+        >>> leaf1 = TreeNode(index=1)
+        >>> leaf2 = TreeNode(index=2)
+        >>> leaf3 = TreeNode(index=3)
+        >>> intr0 = TreeNode([leaf0, leaf2], [0, 0])
+        >>> intr1 = TreeNode([leaf1, leaf3], [0, 0])
+        >>> root  = TreeNode([intr0, intr1], [0, 0])
+        >>> print(leaf0.get_indices())
+        [0]
+        >>> print(intr0.get_indices())
+        [0 2]
+        >>> print(intr1.get_indices())
+        [1 3]
+        >>> print(root.get_indices())
+        [0 2 1 3]
+        """
+        cdef TreeNode leaf
+        return np.array(
+            [leaf._index for leaf in self.get_leaves()], dtype=np.int32
+        )
+
+    def get_leaves(self):
+        """
+        get_leaves()
+        
+        Get a list of leaf nodes that are direct or indirect child nodes
+        of this node.
+
+        This method identifies all leaf nodes, which have this node as
+        ancestor.
+        If this node is a leaf node itself, the list contains this node
+        as single element.
+
+        Returns
+        -------
+        leaf_nodes : list
+            The leaf nodes, that are direct or indirect child nodes
+            of this node.
+        """
+        cdef list leaf_list = []
+        # delegate to 'cdef' method
+        # to reduce overhead of recursive function calling
+        _get_leaves(self, leaf_list)
+        return leaf_list
+    
+    def get_leaf_count(self):
+        """"
+        get_leaf_count()
+
+        Get the number of direct or indirect leaves of this ńode.
+
+        This method identifies all leaf nodes, which have this node as
+        ancestor.
+        If this node is a leaf node itself, 1 is returned.
+        """
+        return _get_leaf_count(self)
+    
+    def to_newick(self, labels=None, bint include_distance=True, 
+                  round_distance=None):
+        """
+        to_newick(labels=None, include_distance=True)
+        
+        Obtain the node represented in Newick notation.
+
+        The terminal semicolon is not included.
+
+        Parameters
+        ----------
+        labels : iterable object of str
+            The labels the indices in the leaf nodes refer to
+        include_distance : bool
+            If true, the distances are displayed in the newick notation,
+            otherwise they are omitted.
+        round_distance : int, optional
+            If set, the distances are rounded to the given number of
+            digits.
+        
+        Returns
+        -------
+        newick : str
+            The Newick notation of the node.
+
+        Examples
+        --------
+        
+        >>> leaf1 = TreeNode(index=0)
+        >>> leaf2 = TreeNode(index=1)
+        >>> leaf3 = TreeNode(index=2)
+        >>> inter = TreeNode([leaf1, leaf2], [5.0, 7.0])
+        >>> root  = TreeNode([inter, leaf3], [3.0, 10.0])
+        >>> print(root.to_newick())
+        ((0:5.0,1:7.0):3.0,2:10.0):0.0
+        >>> print(root.to_newick(include_distance=False))
+        ((0,1),2)
+        >>> labels = ["foo", "bar", "foobar"]
+        >>> print(root.to_newick(labels=labels, include_distance=False))
+        ((foo,bar),foobar)
+        """
+        if self.is_leaf():
+            if labels is not None:
+                for label in labels:
+                    label = labels[self._index]
+                    # Characters that are part of the Newick syntax
+                    # are illegal
+                    illegal_chars = [",",":",";","(",")"]
+                    for char in illegal_chars:
+                        if char in label:
+                            raise ValueError(
+                                f"Label '{label}' contains "
+                                f"illegal character '{char}'"
+                            )
+            else:
+                label = str(self._index)
+            if include_distance:
+                if round_distance is None:
+                    return f"{label}:{self._distance}"
+                else:
+                    return f"{label}:{self._distance:.{round_distance}f}"
+            else:
+                return f"{label}"
+        else:
+            # Build string in a recursive way
+            child_strings = [child.to_newick(
+                labels, include_distance, round_distance
+            ) for child in self._children]
+            if include_distance:
+                if round_distance is None:
+                    return f"({','.join(child_strings)}):{self._distance}"
+                else:
+                    return (
+                        f"({','.join(child_strings)}):"
+                        f"{self._distance:.{round_distance}f}"
+                    )
+            else:
+                return f"({','.join(child_strings)})"
+    
+    @staticmethod
+    def from_newick(str newick, list labels=None):
+        """
+        from_newick(newick, labels=None)
+
+        Create a node and all its child nodes from a Newick notation.
+
+        Parameters
+        ----------
+        newick : str
+            The Newick notation to create the node from.
+        labels : list of str, optional
+            If the Newick notation contains labels, that are not
+            parseable into reference indices,
+            i.e. they are not integers, this parameter can be provided
+            to convert these labels into reference indices.
+            The corresponding index is the position of the label in the
+            provided list.
+
+        Returns
+        -------
+        node : TreeNode
+            The tree node parsed from the Newick notation.
+        distance : float
+            Distance of the node to its parent. If the newick notation
+            does not provide a distance, it is set to 0 by default.
+        
+        Notes
+        -----
+        The provided Newick notation must not have a terminal semicolon.
+        If you have a Newick notation that covers an entire tree, you
+        may use the same method in the :class:`Tree` class instead.
+        Keep in mind that the :class:`TreeNode` class does support any
+        labels on intermediate nodes.
+        If the string contains such labels, they are discarded.
+        """
+        cdef int i
+        cdef int subnewick_start_i = -1
+        cdef int subnewick_stop_i  = -1
+        cdef int level = 0
+        cdef list comma_pos
+        cdef list children
+        cdef list distances
+        cdef int pos
+        cdef int next_pos
+        
+        # Ignore any whitespace
+        newick = "".join(newick.split())
+
+        # Find brackets belonging to sub-newick
+        # e.g. (A:0.1,B:0.2):0.5
+        #      ^           ^
+        for i in range(len(newick)):
+            char = newick[i]
+            if char == "(":
+                subnewick_start_i = i
+                break
+            if char == ")":
+                raise InvalidFileError("Bracket closed before it was opened")
+        for i in reversed(range(len(newick))):
+            char = newick[i]
+            if char == ")":
+                subnewick_stop_i = i+1
+                break
+            if char == "(":
+                raise InvalidFileError("Bracket was opened but not closed")
+        
+        if subnewick_start_i == -1 and subnewick_stop_i == -1:
+            # No brackets -> no sub-newwick -> Leaf node
+            label_and_distance = newick
+            try:
+                label, distance = label_and_distance.split(":")
+                distance = float(distance)
+            except ValueError:
+                # No colon -> No distance is provided
+                distance = 0
+                label = label_and_distance
+            index = int(label) if labels is None else labels.index(label)
+            return TreeNode(index=index), distance
+        
+        else:
+            # Intermediate node
+            if subnewick_stop_i == len(newick):
+                # Node with neither distance nor label
+                label = None
+                distance = 0
+            else:
+                label_and_distance = newick[subnewick_stop_i:]
+                try:
+                    label, distance = label_and_distance.split(":")
+                    distance = float(distance)
+                except ValueError:
+                    # No colon -> No distance is provided
+                    distance = 0
+                    label = label_and_distance
+                # Label of intermediate nodes is discarded 
+                distance = float(distance)
+            
+            subnewick = newick[subnewick_start_i+1 : subnewick_stop_i-1]
+            if len(subnewick) == 0:
+                raise InvalidFileError(
+                    "Intermediate node must at least have one child"
+                )
+            # Parse childs
+            # Split subnewick at ',' if ',' is at current level
+            # (not in a subsubnewick)
+            comma_pos = []
+            for i, char in enumerate(subnewick):
+                if char == "(":
+                    level += 1
+                elif char == ")":
+                    level -= 1
+                elif char == ",":
+                    if level == 0:
+                        comma_pos.append(i)
+                if level < 0:
+                    raise InvalidFileError(
+                        "Bracket closed before it was opened"
+                    )
+        
+            children = []
+            distances = []
+            # Recursive tree construction
+            for i, pos in enumerate(comma_pos):
+                if i == 0:
+                    # (A,B),(C,D),(E,F)
+                    # -----
+                    child, dist = TreeNode.from_newick(
+                        subnewick[:pos], labels=labels
+                    )
+                else:
+                    # (A,B),(C,D),(E,F)
+                    #       -----
+                    prev_pos = comma_pos[i-1]
+                    child, dist = TreeNode.from_newick(
+                        subnewick[prev_pos+1 : pos], labels=labels
+                    )
+                children.append(child)
+                distances.append(dist)
+            # Node after last comma
+            # (A,B),(C,D),(E,F)
+            #             -----
+            if len(comma_pos) != 0:
+                child, dist = TreeNode.from_newick(
+                    subnewick[comma_pos[-1]+1:], labels=labels
+                )
+            else:
+                # Single child node:
+                child, dist = TreeNode.from_newick(
+                    subnewick, labels=labels
+                )
+            children.append(child)
+            distances.append(dist)
+            return TreeNode(children, distances), distance
+
+    def __str__(self):
+        return self.to_newick()
+    
+    def __eq__(self, item):
+        if not isinstance(item, TreeNode):
+            return False
+        cdef TreeNode node = item
+        if self._distance != node._distance:
+            return False
+        if self._index !=-1:
+            if self._index != node._index:
+                return False
+        else:
+            if frozenset(self._children) != frozenset(node._children):
+                return False
+        return True
+    
+    def __hash__(self):
+        # Order of children is not important -> set
+        children_set = frozenset(self._children) \
+                       if self._children is not None else None
+        return hash((self._index, children_set, self._distance))
+
+
+cdef _get_leaves(TreeNode node, list leaf_list):
+    cdef TreeNode child
+    if node._index == -1:
+        # Intermediate node -> Recursive calls
+        for child in node._children:
+            _get_leaves(child, leaf_list)
+    else:
+        # Node itself is leaf node -> add node -> terminate
+        leaf_list.append(node)
+
+
+cdef int _get_leaf_count(TreeNode node):
+    cdef TreeNode child
+    cdef int count = 0
+    if node._index == -1:
+        # Intermediate node -> Recursive calls
+        for child in node._children:
+            count += _get_leaf_count(child)
+        return count
+    else:
+        # Leaf node -> return count of itself = 1
+        return 1
+
+
+cdef list _create_path_to_root(TreeNode node):
+    """
+    Create a list of nodes representing the path from this node to the
+    specified node
+    """
+    cdef list path = []
+    cdef TreeNode current_node = node
+    while current_node is not None:
+        path.append(current_node)
+        current_node = current_node._parent
+    return path
+
+
+
+def as_binary(tree_or_node):
+    """
+    as_binary(tree_or_node)
+
+    Convert a tree into a binary tree.
+
+    In general a :class:`TreeNode` can have more or less than two
+    children.
+    However guide trees usually expect each intermediate node to have
+    exactly two child nodes.
+    This function creates a binary :class:`Tree` (or :class:`TreeNode`)
+    for the given :class:`Tree` (or :class:`TreeNode`):
+    Intermediate nodes that have only a single child are deleted and its
+    parent node is directly connected to its child node.
+    Intermediate nodes that have more than two childs are divided into
+    multiple nodes (distances are preserved).
+    
+    Parameters
+    ----------
+    tree_or_node : Tree or TreeNode
+        The tree or node to be converted into a binary tree or node.
+    
+    Returns
+    -------
+    binary_tree_or_node : Tree or TreeNode
+        The converted tree or node.
+    """
+    if isinstance(tree_or_node, Tree):
+        node, _ = _as_binary(tree_or_node.root)
+        return Tree(node)
+    elif isinstance(tree_or_node, TreeNode):
+        node, _ = _as_binary(tree_or_node)
+        return _as_binary(node)
+    else:
+        raise TypeError(
+            f"Expected 'Tree' or 'TreeNode', not {type(tree_or_node).__name__}"
+        )
+
+cdef _as_binary(TreeNode node):
+    """
+    The actual logic wrapped by :func:`as_binary()`.
+    
+    Parameters
+    ----------
+    node : TreeNode
+        The node to be converted.
+    
+    Returns
+    -------
+    binary_node: TreeNode
+        The converted node.
+    distance : float
+        The distance of the converted node to its parent
+    """
+    cdef TreeNode child
+    cdef TreeNode current_div_node
+    cdef tuple children
+    cdef list rem_children
+    cdef list distances
+    cdef float distance
+
+    children = node.children
+    if children is None:
+        # Leaf node
+        return TreeNode(index=node.index), node.distance
+    elif len(children) == 1:
+        # Intermediate node with one child
+        # -> Omit node and directly connect its child to its parent
+        # The distances are added
+        #
+        #      |--            |--   
+        #      |              |   
+        # --|--|--   ->   ----|--  
+        #      |              |   
+        #      |--            |-- 
+        #
+        child, distance = _as_binary(node.children[0])
+        if node.is_root():
+            # Child is new root -> No distance to parent
+            return child, None
+        else:
+            return child, node.distance + distance
+    elif len(children) > 2:
+        # Intermediate node with more than two childs
+        # -> Create a new node having two childs:
+        #    - One of the childs of the original node
+        #    - The original node with one child less (distance = 0)
+        # Repeat until all children are put into binary nodes
+        #
+        #   |--          |--
+        #   |          --|  |--
+        # --|--   ->     |--|
+        #   |               |--
+        #   |--
+        #
+        # The remaining children
+        rem_children, distances = [list(tup) for tup in zip(
+            *[_as_binary(child) for child in children]
+        )]
+        current_div_node = None
+        while len(rem_children) > 0:
+            if current_div_node is None:
+                # The bottom-most node is created
+                #-> Gets two of the remaining childs
+                current_div_node = TreeNode(
+                    rem_children[:2],
+                    distances[:2]
+                )
+                # Pop the two utilized remaining childs from the list
+                rem_children.pop(0)
+                rem_children.pop(0)
+                distances.pop(0)
+                distances.pop(0)
+            else:
+                # A node is created that gets one remaining child
+                # and the intermediate node from the last step
+                current_div_node = TreeNode(
+                    (current_div_node, rem_children[0]),
+                    (0, distances[0]) 
+                )
+                # Pop the utilized remaining child from the list
+                rem_children.pop(0)
+                distances.pop(0)
+        return current_div_node, node.distance
+    else:
+        # Intermediate node with exactly two childs
+        # -> Keep node unchanged
+        binary_children, distances = [list(tup) for tup in zip(
+            *[_as_binary(child) for child in children]
+        )]
+        return TreeNode(binary_children, distances), node.distance
+
+
+
+class TreeError(Exception):
+    """
+    An exception that occurs in context of tree topology.
+    """
+    pass