@bgicli/bgicli 2.1.1 → 2.2.0
This diff represents the content of publicly available package versions that have been released to one of the supported registries. The information contained in this diff is provided for informational purposes only and reflects changes between package versions as they appear in their respective public registries.
- package/data/skills/aav-vector-design-agent/SKILL.md +198 -0
- package/data/skills/adaptyv/SKILL.md +112 -0
- package/data/skills/adhd-daily-planner/SKILL.md +271 -0
- package/data/skills/aeon/SKILL.md +372 -0
- package/data/skills/agent-browser/SKILL.md +159 -0
- package/data/skills/agentd-drug-discovery/SKILL.md +52 -0
- package/data/skills/ai-analyzer/SKILL.md +218 -0
- package/data/skills/alphafold/SKILL.md +183 -0
- package/data/skills/alphafold-database/SKILL.md +500 -0
- package/data/skills/anndata/SKILL.md +394 -0
- package/data/skills/antibody-design-agent/SKILL.md +64 -0
- package/data/skills/arboreto/SKILL.md +237 -0
- package/data/skills/armored-cart-design-agent/SKILL.md +225 -0
- package/data/skills/arxiv-search/SKILL.md +224 -0
- package/data/skills/autonomous-oncology-agent/SKILL.md +77 -0
- package/data/skills/bayesian-optimizer/SKILL.md +60 -0
- package/data/skills/benchling-integration/SKILL.md +473 -0
- package/data/skills/bgpt-paper-search/SKILL.md +81 -0
- package/data/skills/bindcraft/SKILL.md +198 -0
- package/data/skills/binder-design/SKILL.md +182 -0
- package/data/skills/binding-characterization/SKILL.md +234 -0
- package/data/skills/bindingdb-database/SKILL.md +332 -0
- package/data/skills/bio-admet-prediction/SKILL.md +224 -0
- package/data/skills/bio-alignment-files-bam-statistics/SKILL.md +340 -0
- package/data/skills/bio-alignment-filtering/SKILL.md +322 -0
- package/data/skills/bio-alignment-indexing/SKILL.md +249 -0
- package/data/skills/bio-alignment-io/SKILL.md +301 -0
- package/data/skills/bio-alignment-msa-parsing/SKILL.md +366 -0
- package/data/skills/bio-alignment-msa-statistics/SKILL.md +375 -0
- package/data/skills/bio-alignment-pairwise/SKILL.md +277 -0
- package/data/skills/bio-alignment-sorting/SKILL.md +296 -0
- package/data/skills/bio-alignment-validation/SKILL.md +374 -0
- package/data/skills/bio-atac-seq-atac-peak-calling/SKILL.md +221 -0
- package/data/skills/bio-atac-seq-atac-qc/SKILL.md +292 -0
- package/data/skills/bio-atac-seq-differential-accessibility/SKILL.md +268 -0
- package/data/skills/bio-atac-seq-footprinting/SKILL.md +256 -0
- package/data/skills/bio-atac-seq-motif-deviation/SKILL.md +319 -0
- package/data/skills/bio-atac-seq-nucleosome-positioning/SKILL.md +321 -0
- package/data/skills/bio-basecalling/SKILL.md +368 -0
- package/data/skills/bio-batch-downloads/SKILL.md +384 -0
- package/data/skills/bio-batch-processing/SKILL.md +303 -0
- package/data/skills/bio-bedgraph-handling/SKILL.md +336 -0
- package/data/skills/bio-blast-searches/SKILL.md +354 -0
- package/data/skills/bio-causal-genomics-colocalization-analysis/SKILL.md +264 -0
- package/data/skills/bio-causal-genomics-fine-mapping/SKILL.md +267 -0
- package/data/skills/bio-causal-genomics-mediation-analysis/SKILL.md +264 -0
- package/data/skills/bio-causal-genomics-mendelian-randomization/SKILL.md +221 -0
- package/data/skills/bio-causal-genomics-pleiotropy-detection/SKILL.md +292 -0
- package/data/skills/bio-cfdna-preprocessing/SKILL.md +200 -0
- package/data/skills/bio-chipseq-differential-binding/SKILL.md +262 -0
- package/data/skills/bio-chipseq-motif-analysis/SKILL.md +387 -0
- package/data/skills/bio-chipseq-peak-annotation/SKILL.md +239 -0
- package/data/skills/bio-chipseq-peak-calling/SKILL.md +277 -0
- package/data/skills/bio-chipseq-qc/SKILL.md +391 -0
- package/data/skills/bio-chipseq-super-enhancers/SKILL.md +288 -0
- package/data/skills/bio-chipseq-visualization/SKILL.md +289 -0
- package/data/skills/bio-clinical-databases-clinvar-lookup/SKILL.md +188 -0
- package/data/skills/bio-clinical-databases-dbsnp-queries/SKILL.md +171 -0
- package/data/skills/bio-clinical-databases-gnomad-frequencies/SKILL.md +205 -0
- package/data/skills/bio-clinical-databases-hla-typing/SKILL.md +248 -0
- package/data/skills/bio-clinical-databases-myvariant-queries/SKILL.md +174 -0
- package/data/skills/bio-clinical-databases-pharmacogenomics/SKILL.md +232 -0
- package/data/skills/bio-clinical-databases-polygenic-risk/SKILL.md +276 -0
- package/data/skills/bio-clinical-databases-somatic-signatures/SKILL.md +261 -0
- package/data/skills/bio-clinical-databases-tumor-mutational-burden/SKILL.md +301 -0
- package/data/skills/bio-clinical-databases-variant-prioritization/SKILL.md +225 -0
- package/data/skills/bio-clip-seq-binding-site-annotation/SKILL.md +66 -0
- package/data/skills/bio-clip-seq-clip-alignment/SKILL.md +70 -0
- package/data/skills/bio-clip-seq-clip-motif-analysis/SKILL.md +62 -0
- package/data/skills/bio-clip-seq-clip-peak-calling/SKILL.md +282 -0
- package/data/skills/bio-clip-seq-clip-preprocessing/SKILL.md +142 -0
- package/data/skills/bio-codon-usage/SKILL.md +353 -0
- package/data/skills/bio-comparative-genomics-ancestral-reconstruction/SKILL.md +312 -0
- package/data/skills/bio-comparative-genomics-hgt-detection/SKILL.md +341 -0
- package/data/skills/bio-comparative-genomics-ortholog-inference/SKILL.md +308 -0
- package/data/skills/bio-comparative-genomics-positive-selection/SKILL.md +354 -0
- package/data/skills/bio-comparative-genomics-synteny-analysis/SKILL.md +315 -0
- package/data/skills/bio-compressed-files/SKILL.md +263 -0
- package/data/skills/bio-consensus-sequences/SKILL.md +340 -0
- package/data/skills/bio-copy-number-cnv-annotation/SKILL.md +307 -0
- package/data/skills/bio-copy-number-cnv-visualization/SKILL.md +294 -0
- package/data/skills/bio-copy-number-cnvkit-analysis/SKILL.md +290 -0
- package/data/skills/bio-copy-number-gatk-cnv/SKILL.md +270 -0
- package/data/skills/bio-crispr-screens-base-editing-analysis/SKILL.md +110 -0
- package/data/skills/bio-crispr-screens-batch-correction/SKILL.md +316 -0
- package/data/skills/bio-crispr-screens-crispresso-editing/SKILL.md +205 -0
- package/data/skills/bio-crispr-screens-hit-calling/SKILL.md +264 -0
- package/data/skills/bio-crispr-screens-jacks-analysis/SKILL.md +313 -0
- package/data/skills/bio-crispr-screens-library-design/SKILL.md +417 -0
- package/data/skills/bio-crispr-screens-mageck-analysis/SKILL.md +222 -0
- package/data/skills/bio-crispr-screens-screen-qc/SKILL.md +243 -0
- package/data/skills/bio-ctdna-mutation-detection/SKILL.md +234 -0
- package/data/skills/bio-data-visualization-circos-plots/SKILL.md +405 -0
- package/data/skills/bio-data-visualization-color-palettes/SKILL.md +244 -0
- package/data/skills/bio-data-visualization-genome-browser-tracks/SKILL.md +328 -0
- package/data/skills/bio-data-visualization-genome-tracks/SKILL.md +249 -0
- package/data/skills/bio-data-visualization-ggplot2-fundamentals/SKILL.md +313 -0
- package/data/skills/bio-data-visualization-heatmaps-clustering/SKILL.md +227 -0
- package/data/skills/bio-data-visualization-interactive-visualization/SKILL.md +210 -0
- package/data/skills/bio-data-visualization-multipanel-figures/SKILL.md +274 -0
- package/data/skills/bio-data-visualization-specialized-omics-plots/SKILL.md +251 -0
- package/data/skills/bio-data-visualization-upset-plots/SKILL.md +228 -0
- package/data/skills/bio-data-visualization-volcano-customization/SKILL.md +233 -0
- package/data/skills/bio-de-deseq2-basics/SKILL.md +376 -0
- package/data/skills/bio-de-edger-basics/SKILL.md +418 -0
- package/data/skills/bio-de-results/SKILL.md +378 -0
- package/data/skills/bio-de-visualization/SKILL.md +408 -0
- package/data/skills/bio-differential-expression-batch-correction/SKILL.md +253 -0
- package/data/skills/bio-differential-expression-timeseries-de/SKILL.md +370 -0
- package/data/skills/bio-differential-splicing/SKILL.md +177 -0
- package/data/skills/bio-duplicate-handling/SKILL.md +292 -0
- package/data/skills/bio-entrez-fetch/SKILL.md +334 -0
- package/data/skills/bio-entrez-link/SKILL.md +325 -0
- package/data/skills/bio-entrez-search/SKILL.md +311 -0
- package/data/skills/bio-epidemiological-genomics-amr-surveillance/SKILL.md +233 -0
- package/data/skills/bio-epidemiological-genomics-pathogen-typing/SKILL.md +202 -0
- package/data/skills/bio-epidemiological-genomics-phylodynamics/SKILL.md +207 -0
- package/data/skills/bio-epidemiological-genomics-transmission-inference/SKILL.md +237 -0
- package/data/skills/bio-epidemiological-genomics-variant-surveillance/SKILL.md +237 -0
- package/data/skills/bio-epitranscriptomics-m6a-differential/SKILL.md +88 -0
- package/data/skills/bio-epitranscriptomics-m6a-peak-calling/SKILL.md +89 -0
- package/data/skills/bio-epitranscriptomics-m6anet-analysis/SKILL.md +101 -0
- package/data/skills/bio-epitranscriptomics-merip-preprocessing/SKILL.md +81 -0
- package/data/skills/bio-epitranscriptomics-modification-visualization/SKILL.md +98 -0
- package/data/skills/bio-experimental-design-batch-design/SKILL.md +110 -0
- package/data/skills/bio-experimental-design-multiple-testing/SKILL.md +98 -0
- package/data/skills/bio-experimental-design-power-analysis/SKILL.md +84 -0
- package/data/skills/bio-experimental-design-sample-size/SKILL.md +93 -0
- package/data/skills/bio-expression-matrix-counts-ingest/SKILL.md +220 -0
- package/data/skills/bio-expression-matrix-gene-id-mapping/SKILL.md +256 -0
- package/data/skills/bio-expression-matrix-metadata-joins/SKILL.md +271 -0
- package/data/skills/bio-expression-matrix-sparse-handling/SKILL.md +247 -0
- package/data/skills/bio-fastq-quality/SKILL.md +279 -0
- package/data/skills/bio-filter-sequences/SKILL.md +265 -0
- package/data/skills/bio-flow-cytometry-bead-normalization/SKILL.md +315 -0
- package/data/skills/bio-flow-cytometry-clustering-phenotyping/SKILL.md +237 -0
- package/data/skills/bio-flow-cytometry-compensation-transformation/SKILL.md +196 -0
- package/data/skills/bio-flow-cytometry-cytometry-qc/SKILL.md +382 -0
- package/data/skills/bio-flow-cytometry-differential-analysis/SKILL.md +217 -0
- package/data/skills/bio-flow-cytometry-doublet-detection/SKILL.md +288 -0
- package/data/skills/bio-flow-cytometry-fcs-handling/SKILL.md +221 -0
- package/data/skills/bio-flow-cytometry-gating-analysis/SKILL.md +193 -0
- package/data/skills/bio-format-conversion/SKILL.md +193 -0
- package/data/skills/bio-fragment-analysis/SKILL.md +214 -0
- package/data/skills/bio-gatk-variant-calling/SKILL.md +422 -0
- package/data/skills/bio-genome-assembly-assembly-polishing/SKILL.md +333 -0
- package/data/skills/bio-genome-assembly-assembly-qc/SKILL.md +344 -0
- package/data/skills/bio-genome-assembly-contamination-detection/SKILL.md +235 -0
- package/data/skills/bio-genome-assembly-hifi-assembly/SKILL.md +178 -0
- package/data/skills/bio-genome-assembly-long-read-assembly/SKILL.md +307 -0
- package/data/skills/bio-genome-assembly-metagenome-assembly/SKILL.md +227 -0
- package/data/skills/bio-genome-assembly-scaffolding/SKILL.md +204 -0
- package/data/skills/bio-genome-assembly-short-read-assembly/SKILL.md +319 -0
- package/data/skills/bio-genome-engineering-base-editing-design/SKILL.md +277 -0
- package/data/skills/bio-genome-engineering-grna-design/SKILL.md +221 -0
- package/data/skills/bio-genome-engineering-hdr-template-design/SKILL.md +264 -0
- package/data/skills/bio-genome-engineering-off-target-prediction/SKILL.md +232 -0
- package/data/skills/bio-genome-engineering-prime-editing-design/SKILL.md +275 -0
- package/data/skills/bio-genome-intervals-bed-file-basics/SKILL.md +357 -0
- package/data/skills/bio-genome-intervals-bigwig-tracks/SKILL.md +351 -0
- package/data/skills/bio-genome-intervals-coverage-analysis/SKILL.md +300 -0
- package/data/skills/bio-genome-intervals-gtf-gff-handling/SKILL.md +345 -0
- package/data/skills/bio-genome-intervals-interval-arithmetic/SKILL.md +485 -0
- package/data/skills/bio-genome-intervals-proximity-operations/SKILL.md +337 -0
- package/data/skills/bio-geo-data/SKILL.md +380 -0
- package/data/skills/bio-hi-c-analysis-compartment-analysis/SKILL.md +261 -0
- package/data/skills/bio-hi-c-analysis-contact-pairs/SKILL.md +278 -0
- package/data/skills/bio-hi-c-analysis-hic-data-io/SKILL.md +260 -0
- package/data/skills/bio-hi-c-analysis-hic-differential/SKILL.md +328 -0
- package/data/skills/bio-hi-c-analysis-hic-visualization/SKILL.md +297 -0
- package/data/skills/bio-hi-c-analysis-loop-calling/SKILL.md +284 -0
- package/data/skills/bio-hi-c-analysis-matrix-operations/SKILL.md +274 -0
- package/data/skills/bio-hi-c-analysis-tad-detection/SKILL.md +239 -0
- package/data/skills/bio-imaging-mass-cytometry-cell-segmentation/SKILL.md +241 -0
- package/data/skills/bio-imaging-mass-cytometry-data-preprocessing/SKILL.md +279 -0
- package/data/skills/bio-imaging-mass-cytometry-interactive-annotation/SKILL.md +304 -0
- package/data/skills/bio-imaging-mass-cytometry-phenotyping/SKILL.md +231 -0
- package/data/skills/bio-imaging-mass-cytometry-quality-metrics/SKILL.md +316 -0
- package/data/skills/bio-imaging-mass-cytometry-spatial-analysis/SKILL.md +246 -0
- package/data/skills/bio-immunoinformatics-epitope-prediction/SKILL.md +259 -0
- package/data/skills/bio-immunoinformatics-immunogenicity-scoring/SKILL.md +275 -0
- package/data/skills/bio-immunoinformatics-mhc-binding-prediction/SKILL.md +260 -0
- package/data/skills/bio-immunoinformatics-neoantigen-prediction/SKILL.md +277 -0
- package/data/skills/bio-immunoinformatics-tcr-epitope-binding/SKILL.md +257 -0
- package/data/skills/bio-isoform-switching/SKILL.md +192 -0
- package/data/skills/bio-liquid-biopsy-pipeline/SKILL.md +311 -0
- package/data/skills/bio-local-blast/SKILL.md +350 -0
- package/data/skills/bio-long-read-sequencing-clair3-variants/SKILL.md +252 -0
- package/data/skills/bio-long-read-sequencing-isoseq-analysis/SKILL.md +334 -0
- package/data/skills/bio-long-read-sequencing-nanopore-methylation/SKILL.md +110 -0
- package/data/skills/bio-longitudinal-monitoring/SKILL.md +271 -0
- package/data/skills/bio-longread-alignment/SKILL.md +193 -0
- package/data/skills/bio-longread-medaka/SKILL.md +176 -0
- package/data/skills/bio-longread-qc/SKILL.md +224 -0
- package/data/skills/bio-longread-structural-variants/SKILL.md +201 -0
- package/data/skills/bio-machine-learning-atlas-mapping/SKILL.md +139 -0
- package/data/skills/bio-machine-learning-biomarker-discovery/SKILL.md +157 -0
- package/data/skills/bio-machine-learning-model-validation/SKILL.md +148 -0
- package/data/skills/bio-machine-learning-omics-classifiers/SKILL.md +146 -0
- package/data/skills/bio-machine-learning-prediction-explanation/SKILL.md +162 -0
- package/data/skills/bio-machine-learning-survival-analysis/SKILL.md +176 -0
- package/data/skills/bio-metabolomics-lipidomics/SKILL.md +265 -0
- package/data/skills/bio-metabolomics-metabolite-annotation/SKILL.md +241 -0
- package/data/skills/bio-metabolomics-msdial-preprocessing/SKILL.md +308 -0
- package/data/skills/bio-metabolomics-normalization-qc/SKILL.md +283 -0
- package/data/skills/bio-metabolomics-pathway-mapping/SKILL.md +237 -0
- package/data/skills/bio-metabolomics-statistical-analysis/SKILL.md +276 -0
- package/data/skills/bio-metabolomics-targeted-analysis/SKILL.md +314 -0
- package/data/skills/bio-metabolomics-xcms-preprocessing/SKILL.md +268 -0
- package/data/skills/bio-metagenomics-abundance/SKILL.md +203 -0
- package/data/skills/bio-metagenomics-amr-detection/SKILL.md +293 -0
- package/data/skills/bio-metagenomics-functional-profiling/SKILL.md +252 -0
- package/data/skills/bio-metagenomics-kraken/SKILL.md +204 -0
- package/data/skills/bio-metagenomics-metaphlan/SKILL.md +214 -0
- package/data/skills/bio-metagenomics-strain-tracking/SKILL.md +292 -0
- package/data/skills/bio-metagenomics-visualization/SKILL.md +240 -0
- package/data/skills/bio-methylation-based-detection/SKILL.md +223 -0
- package/data/skills/bio-methylation-bismark-alignment/SKILL.md +195 -0
- package/data/skills/bio-methylation-calling/SKILL.md +200 -0
- package/data/skills/bio-methylation-dmr-detection/SKILL.md +211 -0
- package/data/skills/bio-methylation-methylkit/SKILL.md +219 -0
- package/data/skills/bio-microbiome-amplicon-processing/SKILL.md +137 -0
- package/data/skills/bio-microbiome-differential-abundance/SKILL.md +147 -0
- package/data/skills/bio-microbiome-diversity-analysis/SKILL.md +188 -0
- package/data/skills/bio-microbiome-functional-prediction/SKILL.md +153 -0
- package/data/skills/bio-microbiome-qiime2-workflow/SKILL.md +219 -0
- package/data/skills/bio-microbiome-taxonomy-assignment/SKILL.md +168 -0
- package/data/skills/bio-molecular-descriptors/SKILL.md +200 -0
- package/data/skills/bio-molecular-io/SKILL.md +188 -0
- package/data/skills/bio-motif-search/SKILL.md +354 -0
- package/data/skills/bio-multi-omics-data-harmonization/SKILL.md +228 -0
- package/data/skills/bio-multi-omics-mixomics-analysis/SKILL.md +221 -0
- package/data/skills/bio-multi-omics-mofa-integration/SKILL.md +225 -0
- package/data/skills/bio-multi-omics-similarity-network/SKILL.md +235 -0
- package/data/skills/bio-orchestrator/SKILL.md +133 -0
- package/data/skills/bio-paired-end-fastq/SKILL.md +334 -0
- package/data/skills/bio-pathway-enrichment-visualization/SKILL.md +278 -0
- package/data/skills/bio-pathway-go-enrichment/SKILL.md +218 -0
- package/data/skills/bio-pathway-gsea/SKILL.md +227 -0
- package/data/skills/bio-pathway-kegg-pathways/SKILL.md +234 -0
- package/data/skills/bio-pathway-reactome/SKILL.md +215 -0
- package/data/skills/bio-pathway-wikipathways/SKILL.md +255 -0
- package/data/skills/bio-pdb-geometric-analysis/SKILL.md +475 -0
- package/data/skills/bio-pdb-structure-io/SKILL.md +296 -0
- package/data/skills/bio-pdb-structure-modification/SKILL.md +448 -0
- package/data/skills/bio-pdb-structure-navigation/SKILL.md +335 -0
- package/data/skills/bio-phasing-imputation-genotype-imputation/SKILL.md +201 -0
- package/data/skills/bio-phasing-imputation-haplotype-phasing/SKILL.md +190 -0
- package/data/skills/bio-phasing-imputation-imputation-qc/SKILL.md +265 -0
- package/data/skills/bio-phasing-imputation-reference-panels/SKILL.md +203 -0
- package/data/skills/bio-phylo-distance-calculations/SKILL.md +307 -0
- package/data/skills/bio-phylo-modern-tree-inference/SKILL.md +274 -0
- package/data/skills/bio-phylo-tree-io/SKILL.md +252 -0
- package/data/skills/bio-phylo-tree-manipulation/SKILL.md +375 -0
- package/data/skills/bio-phylo-tree-visualization/SKILL.md +275 -0
- package/data/skills/bio-pileup-generation/SKILL.md +314 -0
- package/data/skills/bio-population-genetics-association-testing/SKILL.md +293 -0
- package/data/skills/bio-population-genetics-linkage-disequilibrium/SKILL.md +260 -0
- package/data/skills/bio-population-genetics-plink-basics/SKILL.md +338 -0
- package/data/skills/bio-population-genetics-population-structure/SKILL.md +352 -0
- package/data/skills/bio-population-genetics-scikit-allel-analysis/SKILL.md +306 -0
- package/data/skills/bio-population-genetics-selection-statistics/SKILL.md +251 -0
- package/data/skills/bio-primer-design-primer-basics/SKILL.md +289 -0
- package/data/skills/bio-primer-design-primer-validation/SKILL.md +344 -0
- package/data/skills/bio-primer-design-qpcr-primers/SKILL.md +273 -0
- package/data/skills/bio-proteomics-data-import/SKILL.md +122 -0
- package/data/skills/bio-proteomics-dia-analysis/SKILL.md +246 -0
- package/data/skills/bio-proteomics-differential-abundance/SKILL.md +129 -0
- package/data/skills/bio-proteomics-peptide-identification/SKILL.md +122 -0
- package/data/skills/bio-proteomics-protein-inference/SKILL.md +174 -0
- package/data/skills/bio-proteomics-proteomics-qc/SKILL.md +208 -0
- package/data/skills/bio-proteomics-ptm-analysis/SKILL.md +139 -0
- package/data/skills/bio-proteomics-quantification/SKILL.md +141 -0
- package/data/skills/bio-proteomics-spectral-libraries/SKILL.md +270 -0
- package/data/skills/bio-reaction-enumeration/SKILL.md +251 -0
- package/data/skills/bio-read-alignment-bowtie2-alignment/SKILL.md +189 -0
- package/data/skills/bio-read-alignment-bwa-alignment/SKILL.md +166 -0
- package/data/skills/bio-read-alignment-hisat2-alignment/SKILL.md +205 -0
- package/data/skills/bio-read-alignment-star-alignment/SKILL.md +204 -0
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---
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name: pyhealth
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description: Comprehensive healthcare AI toolkit for developing, testing, and deploying machine learning models with clinical data. This skill should be used when working with electronic health records (EHR), clinical prediction tasks (mortality, readmission, drug recommendation), medical coding systems (ICD, NDC, ATC), physiological signals (EEG, ECG), healthcare datasets (MIMIC-III/IV, eICU, OMOP), or implementing deep learning models for healthcare applications (RETAIN, SafeDrug, Transformer, GNN).
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---
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# PyHealth: Healthcare AI Toolkit
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## Overview
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PyHealth is a comprehensive Python library for healthcare AI that provides specialized tools, models, and datasets for clinical machine learning. Use this skill when developing healthcare prediction models, processing clinical data, working with medical coding systems, or deploying AI solutions in healthcare settings.
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## When to Use This Skill
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Invoke this skill when:
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- **Working with healthcare datasets**: MIMIC-III, MIMIC-IV, eICU, OMOP, sleep EEG data, medical images
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- **Clinical prediction tasks**: Mortality prediction, hospital readmission, length of stay, drug recommendation
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- **Medical coding**: Translating between ICD-9/10, NDC, RxNorm, ATC coding systems
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- **Processing clinical data**: Sequential events, physiological signals, clinical text, medical images
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- **Implementing healthcare models**: RETAIN, SafeDrug, GAMENet, StageNet, Transformer for EHR
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- **Evaluating clinical models**: Fairness metrics, calibration, interpretability, uncertainty quantification
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## Core Capabilities
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PyHealth operates through a modular 5-stage pipeline optimized for healthcare AI:
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1. **Data Loading**: Access 10+ healthcare datasets with standardized interfaces
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2. **Task Definition**: Apply 20+ predefined clinical prediction tasks or create custom tasks
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3. **Model Selection**: Choose from 33+ models (baselines, deep learning, healthcare-specific)
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4. **Training**: Train with automatic checkpointing, monitoring, and evaluation
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5. **Deployment**: Calibrate, interpret, and validate for clinical use
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**Performance**: 3x faster than pandas for healthcare data processing
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## Quick Start Workflow
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```python
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from pyhealth.datasets import MIMIC4Dataset
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from pyhealth.tasks import mortality_prediction_mimic4_fn
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from pyhealth.datasets import split_by_patient, get_dataloader
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from pyhealth.models import Transformer
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from pyhealth.trainer import Trainer
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# 1. Load dataset and set task
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dataset = MIMIC4Dataset(root="/path/to/data")
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sample_dataset = dataset.set_task(mortality_prediction_mimic4_fn)
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train_loader = get_dataloader(train, batch_size=64, shuffle=True)
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val_loader = get_dataloader(val, batch_size=64, shuffle=False)
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test_loader = get_dataloader(test, batch_size=64, shuffle=False)
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model = Transformer(
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dataset=sample_dataset,
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feature_keys=["diagnoses", "medications"],
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mode="binary",
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embedding_dim=128
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)
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trainer = Trainer(model=model, device="cuda")
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trainer.train(
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train_dataloader=train_loader,
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val_dataloader=val_loader,
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epochs=50,
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monitor="pr_auc_score"
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)
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results = trainer.evaluate(test_loader)
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```
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## Detailed Documentation
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This skill includes comprehensive reference documentation organized by functionality. Read specific reference files as needed:
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### 1. Datasets and Data Structures
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**File**: `references/datasets.md`
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**Read when:**
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- Loading healthcare datasets (MIMIC, eICU, OMOP, sleep EEG, etc.)
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- Understanding Event, Patient, Visit data structures
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- Processing different data types (EHR, signals, images, text)
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**Key Topics:**
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- Core data structures (Event, Patient, Visit)
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- 10+ available datasets (EHR, physiological signals, imaging, text)
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- Data loading and iteration
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- Train/val/test splitting strategies
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- Translating between medical coding systems
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- Working with diagnosis codes (ICD-9-CM, ICD-10-CM, CCS)
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- Processing medication codes (NDC, RxNorm, ATC)
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- Standardizing procedure codes (ICD-9-PROC, ICD-10-PROC)
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**Read when:**
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- Defining clinical prediction objectives
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- Handling sequential events and time-series data
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- Processing physiological signals (EEG, ECG)
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- Normalizing lab values and vital signs
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- Preparing labels for different task types
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- Building feature vocabularies
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- Managing missing data and outliers
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**Key Topics:**
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- 15+ processor types
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- Sequence processing (padding, truncation)
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- Signal processing (filtering, segmentation)
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- Feature extraction and encoding
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- Label processors (binary, multi-class, multi-label, regression)
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- Text and image preprocessing
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- Common preprocessing workflows
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### 6. Training and Evaluation
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**File**: `references/training_evaluation.md`
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**Read when:**
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- Training models with the Trainer class
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- Evaluating model performance
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- Computing clinical metrics
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- Assessing model fairness across demographics
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- Calibrating predictions for reliability
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- Quantifying prediction uncertainty
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- Interpreting model predictions
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- Preparing models for clinical deployment
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**Key Topics:**
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- Trainer class (train, evaluate, inference)
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- Metrics for binary, multi-class, multi-label, regression tasks
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- Fairness metrics for bias assessment
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- Calibration methods (Platt scaling, temperature scaling)
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- Uncertainty quantification (conformal prediction, MC dropout)
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- Interpretability tools (attention visualization, SHAP, ChEFER)
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- Complete training pipeline example
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## Installation
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```bash
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uv pip install pyhealth
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```
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**Requirements:**
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- Python ≥ 3.7
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- PyTorch ≥ 1.8
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- NumPy, pandas, scikit-learn
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## Common Use Cases
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### Use Case 1: ICU Mortality Prediction
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**Objective**: Predict patient mortality in intensive care unit
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**Approach:**
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1. Load MIMIC-IV dataset → Read `references/datasets.md`
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2. Apply mortality prediction task → Read `references/tasks.md`
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3. Select interpretable model (RETAIN) → Read `references/models.md`
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4. Train and evaluate → Read `references/training_evaluation.md`
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5. Interpret predictions for clinical use → Read `references/training_evaluation.md`
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### Use Case 2: Safe Medication Recommendation
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**Objective**: Recommend medications while avoiding drug-drug interactions
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**Approach:**
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1. Load EHR dataset (MIMIC-IV or OMOP) → Read `references/datasets.md`
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2. Apply drug recommendation task → Read `references/tasks.md`
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3. Use SafeDrug model with DDI constraints → Read `references/models.md`
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4. Preprocess medication codes → Read `references/medical_coding.md`
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5. Evaluate with multi-label metrics → Read `references/training_evaluation.md`
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### Use Case 3: Hospital Readmission Prediction
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**Objective**: Identify patients at risk of 30-day readmission
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**Approach:**
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1. Load multi-site EHR data (eICU or OMOP) → Read `references/datasets.md`
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2. Apply readmission prediction task → Read `references/tasks.md`
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3. Handle class imbalance in preprocessing → Read `references/preprocessing.md`
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4. Train Transformer model → Read `references/models.md`
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5. Calibrate predictions and assess fairness → Read `references/training_evaluation.md`
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### Use Case 4: Sleep Disorder Diagnosis
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**Objective**: Classify sleep stages from EEG signals
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**Approach:**
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1. Load sleep EEG dataset (SleepEDF, SHHS) → Read `references/datasets.md`
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2. Apply sleep staging task → Read `references/tasks.md`
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3. Preprocess EEG signals (filtering, segmentation) → Read `references/preprocessing.md`
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4. Train CNN or RNN model → Read `references/models.md`
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5. Evaluate per-stage performance → Read `references/training_evaluation.md`
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### Use Case 5: Medical Code Translation
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**Objective**: Standardize diagnoses across different coding systems
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**Approach:**
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1. Read `references/medical_coding.md` for comprehensive guidance
|
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2. Use CrossMap to translate between ICD-9, ICD-10, CCS
|
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3. Group codes into clinically meaningful categories
|
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4. Integrate with dataset processing
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### Use Case 6: Clinical Text to ICD Coding
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**Objective**: Automatically assign ICD codes from clinical notes
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+
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**Approach:**
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1. Load MIMIC-III with clinical text → Read `references/datasets.md`
|
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278
|
+
2. Apply ICD coding task → Read `references/tasks.md`
|
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279
|
+
3. Preprocess clinical text → Read `references/preprocessing.md`
|
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280
|
+
4. Use TransformersModel (ClinicalBERT) → Read `references/models.md`
|
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281
|
+
5. Evaluate with multi-label metrics → Read `references/training_evaluation.md`
|
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|
+
|
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## Best Practices
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|
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### Data Handling
|
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|
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|
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|
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1. **Always split by patient**: Prevent data leakage by ensuring no patient appears in multiple splits
|
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```python
|
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|
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from pyhealth.datasets import split_by_patient
|
|
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|
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train, val, test = split_by_patient(dataset, [0.7, 0.1, 0.2])
|
|
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|
+
```
|
|
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|
+
|
|
293
|
+
2. **Check dataset statistics**: Understand your data before modeling
|
|
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|
+
```python
|
|
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|
+
print(dataset.stats()) # Patients, visits, events, code distributions
|
|
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|
+
```
|
|
297
|
+
|
|
298
|
+
3. **Use appropriate preprocessing**: Match processors to data types (see `references/preprocessing.md`)
|
|
299
|
+
|
|
300
|
+
### Model Development
|
|
301
|
+
|
|
302
|
+
1. **Start with baselines**: Establish baseline performance with simple models
|
|
303
|
+
- Logistic Regression for binary/multi-class tasks
|
|
304
|
+
- MLP for initial deep learning baseline
|
|
305
|
+
|
|
306
|
+
2. **Choose task-appropriate models**:
|
|
307
|
+
- Interpretability needed → RETAIN, AdaCare
|
|
308
|
+
- Drug recommendation → SafeDrug, GAMENet
|
|
309
|
+
- Long sequences → Transformer
|
|
310
|
+
- Graph relationships → GNN
|
|
311
|
+
|
|
312
|
+
3. **Monitor validation metrics**: Use appropriate metrics for task and handle class imbalance
|
|
313
|
+
- Binary classification: AUROC, AUPRC (especially for rare events)
|
|
314
|
+
- Multi-class: macro-F1 (for imbalanced), weighted-F1
|
|
315
|
+
- Multi-label: Jaccard, example-F1
|
|
316
|
+
- Regression: MAE, RMSE
|
|
317
|
+
|
|
318
|
+
### Clinical Deployment
|
|
319
|
+
|
|
320
|
+
1. **Calibrate predictions**: Ensure probabilities are reliable (see `references/training_evaluation.md`)
|
|
321
|
+
|
|
322
|
+
2. **Assess fairness**: Evaluate across demographic groups to detect bias
|
|
323
|
+
|
|
324
|
+
3. **Quantify uncertainty**: Provide confidence estimates for predictions
|
|
325
|
+
|
|
326
|
+
4. **Interpret predictions**: Use attention weights, SHAP, or ChEFER for clinical trust
|
|
327
|
+
|
|
328
|
+
5. **Validate thoroughly**: Use held-out test sets from different time periods or sites
|
|
329
|
+
|
|
330
|
+
## Limitations and Considerations
|
|
331
|
+
|
|
332
|
+
### Data Requirements
|
|
333
|
+
|
|
334
|
+
- **Large datasets**: Deep learning models require sufficient data (thousands of patients)
|
|
335
|
+
- **Data quality**: Missing data and coding errors impact performance
|
|
336
|
+
- **Temporal consistency**: Ensure train/test split respects temporal ordering when needed
|
|
337
|
+
|
|
338
|
+
### Clinical Validation
|
|
339
|
+
|
|
340
|
+
- **External validation**: Test on data from different hospitals/systems
|
|
341
|
+
- **Prospective evaluation**: Validate in real clinical settings before deployment
|
|
342
|
+
- **Clinical review**: Have clinicians review predictions and interpretations
|
|
343
|
+
- **Ethical considerations**: Address privacy (HIPAA/GDPR), fairness, and safety
|
|
344
|
+
|
|
345
|
+
### Computational Resources
|
|
346
|
+
|
|
347
|
+
- **GPU recommended**: For training deep learning models efficiently
|
|
348
|
+
- **Memory requirements**: Large datasets may require 16GB+ RAM
|
|
349
|
+
- **Storage**: Healthcare datasets can be 10s-100s of GB
|
|
350
|
+
|
|
351
|
+
## Troubleshooting
|
|
352
|
+
|
|
353
|
+
### Common Issues
|
|
354
|
+
|
|
355
|
+
**ImportError for dataset**:
|
|
356
|
+
- Ensure dataset files are downloaded and path is correct
|
|
357
|
+
- Check PyHealth version compatibility
|
|
358
|
+
|
|
359
|
+
**Out of memory**:
|
|
360
|
+
- Reduce batch size
|
|
361
|
+
- Reduce sequence length (`max_seq_length`)
|
|
362
|
+
- Use gradient accumulation
|
|
363
|
+
- Process data in chunks
|
|
364
|
+
|
|
365
|
+
**Poor performance**:
|
|
366
|
+
- Check class imbalance and use appropriate metrics (AUPRC vs AUROC)
|
|
367
|
+
- Verify preprocessing (normalization, missing data handling)
|
|
368
|
+
- Increase model capacity or training epochs
|
|
369
|
+
- Check for data leakage in train/test split
|
|
370
|
+
|
|
371
|
+
**Slow training**:
|
|
372
|
+
- Use GPU (`device="cuda"`)
|
|
373
|
+
- Increase batch size (if memory allows)
|
|
374
|
+
- Reduce sequence length
|
|
375
|
+
- Use more efficient model (CNN vs Transformer)
|
|
376
|
+
|
|
377
|
+
### Getting Help
|
|
378
|
+
|
|
379
|
+
- **Documentation**: https://pyhealth.readthedocs.io/
|
|
380
|
+
- **GitHub Issues**: https://github.com/sunlabuiuc/PyHealth/issues
|
|
381
|
+
- **Tutorials**: 7 core tutorials + 5 practical pipelines available online
|
|
382
|
+
|
|
383
|
+
## Example: Complete Workflow
|
|
384
|
+
|
|
385
|
+
```python
|
|
386
|
+
# Complete mortality prediction pipeline
|
|
387
|
+
from pyhealth.datasets import MIMIC4Dataset
|
|
388
|
+
from pyhealth.tasks import mortality_prediction_mimic4_fn
|
|
389
|
+
from pyhealth.datasets import split_by_patient, get_dataloader
|
|
390
|
+
from pyhealth.models import RETAIN
|
|
391
|
+
from pyhealth.trainer import Trainer
|
|
392
|
+
|
|
393
|
+
# 1. Load dataset
|
|
394
|
+
print("Loading MIMIC-IV dataset...")
|
|
395
|
+
dataset = MIMIC4Dataset(root="/data/mimic4")
|
|
396
|
+
print(dataset.stats())
|
|
397
|
+
|
|
398
|
+
# 2. Define task
|
|
399
|
+
print("Setting mortality prediction task...")
|
|
400
|
+
sample_dataset = dataset.set_task(mortality_prediction_mimic4_fn)
|
|
401
|
+
print(f"Generated {len(sample_dataset)} samples")
|
|
402
|
+
|
|
403
|
+
# 3. Split data (by patient to prevent leakage)
|
|
404
|
+
print("Splitting data...")
|
|
405
|
+
train_ds, val_ds, test_ds = split_by_patient(
|
|
406
|
+
sample_dataset, ratios=[0.7, 0.1, 0.2], seed=42
|
|
407
|
+
)
|
|
408
|
+
|
|
409
|
+
# 4. Create data loaders
|
|
410
|
+
train_loader = get_dataloader(train_ds, batch_size=64, shuffle=True)
|
|
411
|
+
val_loader = get_dataloader(val_ds, batch_size=64)
|
|
412
|
+
test_loader = get_dataloader(test_ds, batch_size=64)
|
|
413
|
+
|
|
414
|
+
# 5. Initialize interpretable model
|
|
415
|
+
print("Initializing RETAIN model...")
|
|
416
|
+
model = RETAIN(
|
|
417
|
+
dataset=sample_dataset,
|
|
418
|
+
feature_keys=["diagnoses", "procedures", "medications"],
|
|
419
|
+
mode="binary",
|
|
420
|
+
embedding_dim=128,
|
|
421
|
+
hidden_dim=128
|
|
422
|
+
)
|
|
423
|
+
|
|
424
|
+
# 6. Train model
|
|
425
|
+
print("Training model...")
|
|
426
|
+
trainer = Trainer(model=model, device="cuda")
|
|
427
|
+
trainer.train(
|
|
428
|
+
train_dataloader=train_loader,
|
|
429
|
+
val_dataloader=val_loader,
|
|
430
|
+
epochs=50,
|
|
431
|
+
optimizer="Adam",
|
|
432
|
+
learning_rate=1e-3,
|
|
433
|
+
weight_decay=1e-5,
|
|
434
|
+
monitor="pr_auc_score", # Use AUPRC for imbalanced data
|
|
435
|
+
monitor_criterion="max",
|
|
436
|
+
save_path="./checkpoints/mortality_retain"
|
|
437
|
+
)
|
|
438
|
+
|
|
439
|
+
# 7. Evaluate on test set
|
|
440
|
+
print("Evaluating on test set...")
|
|
441
|
+
test_results = trainer.evaluate(
|
|
442
|
+
test_loader,
|
|
443
|
+
metrics=["accuracy", "precision", "recall", "f1_score",
|
|
444
|
+
"roc_auc_score", "pr_auc_score"]
|
|
445
|
+
)
|
|
446
|
+
|
|
447
|
+
print("\nTest Results:")
|
|
448
|
+
for metric, value in test_results.items():
|
|
449
|
+
print(f" {metric}: {value:.4f}")
|
|
450
|
+
|
|
451
|
+
# 8. Get predictions with attention for interpretation
|
|
452
|
+
predictions = trainer.inference(
|
|
453
|
+
test_loader,
|
|
454
|
+
additional_outputs=["visit_attention", "feature_attention"],
|
|
455
|
+
return_patient_ids=True
|
|
456
|
+
)
|
|
457
|
+
|
|
458
|
+
# 9. Analyze a high-risk patient
|
|
459
|
+
high_risk_idx = predictions["y_pred"].argmax()
|
|
460
|
+
patient_id = predictions["patient_ids"][high_risk_idx]
|
|
461
|
+
visit_attn = predictions["visit_attention"][high_risk_idx]
|
|
462
|
+
feature_attn = predictions["feature_attention"][high_risk_idx]
|
|
463
|
+
|
|
464
|
+
print(f"\nHigh-risk patient: {patient_id}")
|
|
465
|
+
print(f"Risk score: {predictions['y_pred'][high_risk_idx]:.3f}")
|
|
466
|
+
print(f"Most influential visit: {visit_attn.argmax()}")
|
|
467
|
+
print(f"Most important features: {feature_attn[visit_attn.argmax()].argsort()[-5:]}")
|
|
468
|
+
|
|
469
|
+
# 10. Save model for deployment
|
|
470
|
+
trainer.save("./models/mortality_retain_final.pt")
|
|
471
|
+
print("\nModel saved successfully!")
|
|
472
|
+
```
|
|
473
|
+
|
|
474
|
+
## Resources
|
|
475
|
+
|
|
476
|
+
For detailed information on each component, refer to the comprehensive reference files in the `references/` directory:
|
|
477
|
+
|
|
478
|
+
- **datasets.md**: Data structures, loading, and splitting (4,500 words)
|
|
479
|
+
- **medical_coding.md**: Code translation and standardization (3,800 words)
|
|
480
|
+
- **tasks.md**: Clinical prediction tasks and custom task creation (4,200 words)
|
|
481
|
+
- **models.md**: Model architectures and selection guidelines (5,100 words)
|
|
482
|
+
- **preprocessing.md**: Data processors and preprocessing workflows (4,600 words)
|
|
483
|
+
- **training_evaluation.md**: Training, metrics, calibration, interpretability (5,900 words)
|
|
484
|
+
|
|
485
|
+
**Total comprehensive documentation**: ~28,000 words across modular reference files.
|
|
@@ -0,0 +1,179 @@
|
|
|
1
|
+
---
|
|
2
|
+
name: pylabrobot
|
|
3
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description: Laboratory automation toolkit for controlling liquid handlers, plate readers, pumps, heater shakers, incubators, centrifuges, and analytical equipment. Use this skill when automating laboratory workflows, programming liquid handling robots (Hamilton STAR, Opentrons OT-2, Tecan EVO), integrating lab equipment, managing deck layouts and resources (plates, tips, containers), reading plates, or creating reproducible laboratory protocols. Applicable for both simulated protocols and physical hardware control.
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---
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# PyLabRobot
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## Overview
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PyLabRobot is a hardware-agnostic, pure Python Software Development Kit for automated and autonomous laboratories. Use this skill to control liquid handling robots, plate readers, pumps, heater shakers, incubators, centrifuges, and other laboratory automation equipment through a unified Python interface that works across platforms (Windows, macOS, Linux).
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## When to Use This Skill
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Use this skill when:
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- Programming liquid handling robots (Hamilton STAR/STARlet, Opentrons OT-2, Tecan EVO)
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- Automating laboratory workflows involving pipetting, sample preparation, or analytical measurements
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- Managing deck layouts and laboratory resources (plates, tips, containers, troughs)
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- Integrating multiple lab devices (liquid handlers, plate readers, heater shakers, pumps)
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- Creating reproducible laboratory protocols with state management
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- Simulating protocols before running on physical hardware
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- Reading plates using BMG CLARIOstar or other supported plate readers
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- Controlling temperature, shaking, centrifugation, or other material handling operations
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- Working with laboratory automation in Python
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## Core Capabilities
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PyLabRobot provides comprehensive laboratory automation through six main capability areas, each detailed in the references/ directory:
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### 1. Liquid Handling (`references/liquid-handling.md`)
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Control liquid handling robots for aspirating, dispensing, and transferring liquids. Key operations include:
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- **Basic Operations**: Aspirate, dispense, transfer liquids between wells
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- **Tip Management**: Pick up, drop, and track pipette tips automatically
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- **Advanced Techniques**: Multi-channel pipetting, serial dilutions, plate replication
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- **Volume Tracking**: Automatic tracking of liquid volumes in wells
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- **Hardware Support**: Hamilton STAR/STARlet, Opentrons OT-2, Tecan EVO, and others
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### 2. Resource Management (`references/resources.md`)
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Manage laboratory resources in a hierarchical system:
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- **Resource Types**: Plates, tip racks, troughs, tubes, carriers, and custom labware
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- **Deck Layout**: Assign resources to deck positions with coordinate systems
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- **State Management**: Track tip presence, liquid volumes, and resource states
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- **Serialization**: Save and load deck layouts and states from JSON files
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- **Resource Discovery**: Access wells, tips, and containers through intuitive APIs
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### 3. Hardware Backends (`references/hardware-backends.md`)
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Connect to diverse laboratory equipment through backend abstraction:
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- **Liquid Handlers**: Hamilton STAR (full support), Opentrons OT-2, Tecan EVO
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- **Simulation**: ChatterboxBackend for protocol testing without hardware
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- **Platform Support**: Works on Windows, macOS, Linux, and Raspberry Pi
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- **Backend Switching**: Change robots by swapping backend without rewriting protocols
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### 4. Analytical Equipment (`references/analytical-equipment.md`)
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Integrate plate readers and analytical instruments:
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- **Plate Readers**: BMG CLARIOstar for absorbance, luminescence, fluorescence
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- **Scales**: Mettler Toledo integration for mass measurements
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- **Integration Patterns**: Combine liquid handlers with analytical equipment
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- **Automated Workflows**: Move plates between devices automatically
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### 5. Material Handling (`references/material-handling.md`)
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Control environmental and material handling equipment:
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- **Heater Shakers**: Hamilton HeaterShaker, Inheco ThermoShake
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- **Incubators**: Inheco and Thermo Fisher incubators with temperature control
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- **Centrifuges**: Agilent VSpin with bucket positioning and spin control
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- **Pumps**: Cole Parmer Masterflex for fluid pumping operations
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- **Temperature Control**: Set and monitor temperatures during protocols
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### 6. Visualization & Simulation (`references/visualization.md`)
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Visualize and simulate laboratory protocols:
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- **Browser Visualizer**: Real-time 3D visualization of deck state
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- **Simulation Mode**: Test protocols without physical hardware
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- **State Tracking**: Monitor tip presence and liquid volumes visually
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- **Deck Editor**: Graphical tool for designing deck layouts
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- **Protocol Validation**: Verify protocols before running on hardware
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## Quick Start
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To get started with PyLabRobot, install the package and initialize a liquid handler:
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```python
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# Install PyLabRobot
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# uv pip install pylabrobot
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# Basic liquid handling setup
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from pylabrobot.liquid_handling import LiquidHandler
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from pylabrobot.liquid_handling.backends import STAR
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from pylabrobot.resources import STARLetDeck
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# Initialize liquid handler
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lh = LiquidHandler(backend=STAR(), deck=STARLetDeck())
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await lh.setup()
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# Basic operations
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await lh.pick_up_tips(tip_rack["A1:H1"])
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await lh.aspirate(plate["A1"], vols=100)
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await lh.dispense(plate["A2"], vols=100)
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await lh.drop_tips()
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```
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## Working with References
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This skill organizes detailed information across multiple reference files. Load the relevant reference when:
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- **Liquid Handling**: Writing pipetting protocols, tip management, transfers
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- **Resources**: Defining deck layouts, managing plates/tips, custom labware
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- **Hardware Backends**: Connecting to specific robots, switching platforms
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- **Analytical Equipment**: Integrating plate readers, scales, or analytical devices
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- **Material Handling**: Using heater shakers, incubators, centrifuges, pumps
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- **Visualization**: Simulating protocols, visualizing deck states
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All reference files can be found in the `references/` directory and contain comprehensive examples, API usage patterns, and best practices.
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## Best Practices
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When creating laboratory automation protocols with PyLabRobot:
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1. **Start with Simulation**: Use ChatterboxBackend and the visualizer to test protocols before running on hardware
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2. **Enable Tracking**: Turn on tip tracking and volume tracking for accurate state management
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3. **Resource Naming**: Use clear, descriptive names for all resources (plates, tip racks, containers)
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4. **State Serialization**: Save deck layouts and states to JSON for reproducibility
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5. **Error Handling**: Implement proper async error handling for hardware operations
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6. **Temperature Control**: Set temperatures early as heating/cooling takes time
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7. **Modular Protocols**: Break complex workflows into reusable functions
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8. **Documentation**: Reference official docs at https://docs.pylabrobot.org for latest features
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## Common Workflows
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### Liquid Transfer Protocol
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```python
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# Setup
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lh = LiquidHandler(backend=STAR(), deck=STARLetDeck())
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await lh.setup()
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# Define resources
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tip_rack = TIP_CAR_480_A00(name="tip_rack")
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source_plate = Cos_96_DW_1mL(name="source")
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dest_plate = Cos_96_DW_1mL(name="dest")
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lh.deck.assign_child_resource(tip_rack, rails=1)
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lh.deck.assign_child_resource(source_plate, rails=10)
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lh.deck.assign_child_resource(dest_plate, rails=15)
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# Transfer protocol
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await lh.pick_up_tips(tip_rack["A1:H1"])
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await lh.transfer(source_plate["A1:H12"], dest_plate["A1:H12"], vols=100)
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await lh.drop_tips()
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```
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### Plate Reading Workflow
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```python
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# Setup plate reader
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from pylabrobot.plate_reading import PlateReader
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from pylabrobot.plate_reading.clario_star_backend import CLARIOstarBackend
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pr = PlateReader(name="CLARIOstar", backend=CLARIOstarBackend())
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await pr.setup()
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# Set temperature and read
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await pr.set_temperature(37)
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await pr.open()
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# (manually or robotically load plate)
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await pr.close()
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data = await pr.read_absorbance(wavelength=450)
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```
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## Additional Resources
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- **Official Documentation**: https://docs.pylabrobot.org
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- **GitHub Repository**: https://github.com/PyLabRobot/pylabrobot
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- **Community Forum**: https://discuss.pylabrobot.org
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- **PyPI Package**: https://pypi.org/project/PyLabRobot/
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For detailed usage of specific capabilities, refer to the corresponding reference file in the `references/` directory.
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