@bgicli/bgicli 2.1.1 → 2.2.0

This diff represents the content of publicly available package versions that have been released to one of the supported registries. The information contained in this diff is provided for informational purposes only and reflects changes between package versions as they appear in their respective public registries.
Files changed (1266) hide show
  1. package/data/skills/aav-vector-design-agent/SKILL.md +198 -0
  2. package/data/skills/adaptyv/SKILL.md +112 -0
  3. package/data/skills/adhd-daily-planner/SKILL.md +271 -0
  4. package/data/skills/aeon/SKILL.md +372 -0
  5. package/data/skills/agent-browser/SKILL.md +159 -0
  6. package/data/skills/agentd-drug-discovery/SKILL.md +52 -0
  7. package/data/skills/ai-analyzer/SKILL.md +218 -0
  8. package/data/skills/alphafold/SKILL.md +183 -0
  9. package/data/skills/alphafold-database/SKILL.md +500 -0
  10. package/data/skills/anndata/SKILL.md +394 -0
  11. package/data/skills/antibody-design-agent/SKILL.md +64 -0
  12. package/data/skills/arboreto/SKILL.md +237 -0
  13. package/data/skills/armored-cart-design-agent/SKILL.md +225 -0
  14. package/data/skills/arxiv-search/SKILL.md +224 -0
  15. package/data/skills/autonomous-oncology-agent/SKILL.md +77 -0
  16. package/data/skills/bayesian-optimizer/SKILL.md +60 -0
  17. package/data/skills/benchling-integration/SKILL.md +473 -0
  18. package/data/skills/bgpt-paper-search/SKILL.md +81 -0
  19. package/data/skills/bindcraft/SKILL.md +198 -0
  20. package/data/skills/binder-design/SKILL.md +182 -0
  21. package/data/skills/binding-characterization/SKILL.md +234 -0
  22. package/data/skills/bindingdb-database/SKILL.md +332 -0
  23. package/data/skills/bio-admet-prediction/SKILL.md +224 -0
  24. package/data/skills/bio-alignment-files-bam-statistics/SKILL.md +340 -0
  25. package/data/skills/bio-alignment-filtering/SKILL.md +322 -0
  26. package/data/skills/bio-alignment-indexing/SKILL.md +249 -0
  27. package/data/skills/bio-alignment-io/SKILL.md +301 -0
  28. package/data/skills/bio-alignment-msa-parsing/SKILL.md +366 -0
  29. package/data/skills/bio-alignment-msa-statistics/SKILL.md +375 -0
  30. package/data/skills/bio-alignment-pairwise/SKILL.md +277 -0
  31. package/data/skills/bio-alignment-sorting/SKILL.md +296 -0
  32. package/data/skills/bio-alignment-validation/SKILL.md +374 -0
  33. package/data/skills/bio-atac-seq-atac-peak-calling/SKILL.md +221 -0
  34. package/data/skills/bio-atac-seq-atac-qc/SKILL.md +292 -0
  35. package/data/skills/bio-atac-seq-differential-accessibility/SKILL.md +268 -0
  36. package/data/skills/bio-atac-seq-footprinting/SKILL.md +256 -0
  37. package/data/skills/bio-atac-seq-motif-deviation/SKILL.md +319 -0
  38. package/data/skills/bio-atac-seq-nucleosome-positioning/SKILL.md +321 -0
  39. package/data/skills/bio-basecalling/SKILL.md +368 -0
  40. package/data/skills/bio-batch-downloads/SKILL.md +384 -0
  41. package/data/skills/bio-batch-processing/SKILL.md +303 -0
  42. package/data/skills/bio-bedgraph-handling/SKILL.md +336 -0
  43. package/data/skills/bio-blast-searches/SKILL.md +354 -0
  44. package/data/skills/bio-causal-genomics-colocalization-analysis/SKILL.md +264 -0
  45. package/data/skills/bio-causal-genomics-fine-mapping/SKILL.md +267 -0
  46. package/data/skills/bio-causal-genomics-mediation-analysis/SKILL.md +264 -0
  47. package/data/skills/bio-causal-genomics-mendelian-randomization/SKILL.md +221 -0
  48. package/data/skills/bio-causal-genomics-pleiotropy-detection/SKILL.md +292 -0
  49. package/data/skills/bio-cfdna-preprocessing/SKILL.md +200 -0
  50. package/data/skills/bio-chipseq-differential-binding/SKILL.md +262 -0
  51. package/data/skills/bio-chipseq-motif-analysis/SKILL.md +387 -0
  52. package/data/skills/bio-chipseq-peak-annotation/SKILL.md +239 -0
  53. package/data/skills/bio-chipseq-peak-calling/SKILL.md +277 -0
  54. package/data/skills/bio-chipseq-qc/SKILL.md +391 -0
  55. package/data/skills/bio-chipseq-super-enhancers/SKILL.md +288 -0
  56. package/data/skills/bio-chipseq-visualization/SKILL.md +289 -0
  57. package/data/skills/bio-clinical-databases-clinvar-lookup/SKILL.md +188 -0
  58. package/data/skills/bio-clinical-databases-dbsnp-queries/SKILL.md +171 -0
  59. package/data/skills/bio-clinical-databases-gnomad-frequencies/SKILL.md +205 -0
  60. package/data/skills/bio-clinical-databases-hla-typing/SKILL.md +248 -0
  61. package/data/skills/bio-clinical-databases-myvariant-queries/SKILL.md +174 -0
  62. package/data/skills/bio-clinical-databases-pharmacogenomics/SKILL.md +232 -0
  63. package/data/skills/bio-clinical-databases-polygenic-risk/SKILL.md +276 -0
  64. package/data/skills/bio-clinical-databases-somatic-signatures/SKILL.md +261 -0
  65. package/data/skills/bio-clinical-databases-tumor-mutational-burden/SKILL.md +301 -0
  66. package/data/skills/bio-clinical-databases-variant-prioritization/SKILL.md +225 -0
  67. package/data/skills/bio-clip-seq-binding-site-annotation/SKILL.md +66 -0
  68. package/data/skills/bio-clip-seq-clip-alignment/SKILL.md +70 -0
  69. package/data/skills/bio-clip-seq-clip-motif-analysis/SKILL.md +62 -0
  70. package/data/skills/bio-clip-seq-clip-peak-calling/SKILL.md +282 -0
  71. package/data/skills/bio-clip-seq-clip-preprocessing/SKILL.md +142 -0
  72. package/data/skills/bio-codon-usage/SKILL.md +353 -0
  73. package/data/skills/bio-comparative-genomics-ancestral-reconstruction/SKILL.md +312 -0
  74. package/data/skills/bio-comparative-genomics-hgt-detection/SKILL.md +341 -0
  75. package/data/skills/bio-comparative-genomics-ortholog-inference/SKILL.md +308 -0
  76. package/data/skills/bio-comparative-genomics-positive-selection/SKILL.md +354 -0
  77. package/data/skills/bio-comparative-genomics-synteny-analysis/SKILL.md +315 -0
  78. package/data/skills/bio-compressed-files/SKILL.md +263 -0
  79. package/data/skills/bio-consensus-sequences/SKILL.md +340 -0
  80. package/data/skills/bio-copy-number-cnv-annotation/SKILL.md +307 -0
  81. package/data/skills/bio-copy-number-cnv-visualization/SKILL.md +294 -0
  82. package/data/skills/bio-copy-number-cnvkit-analysis/SKILL.md +290 -0
  83. package/data/skills/bio-copy-number-gatk-cnv/SKILL.md +270 -0
  84. package/data/skills/bio-crispr-screens-base-editing-analysis/SKILL.md +110 -0
  85. package/data/skills/bio-crispr-screens-batch-correction/SKILL.md +316 -0
  86. package/data/skills/bio-crispr-screens-crispresso-editing/SKILL.md +205 -0
  87. package/data/skills/bio-crispr-screens-hit-calling/SKILL.md +264 -0
  88. package/data/skills/bio-crispr-screens-jacks-analysis/SKILL.md +313 -0
  89. package/data/skills/bio-crispr-screens-library-design/SKILL.md +417 -0
  90. package/data/skills/bio-crispr-screens-mageck-analysis/SKILL.md +222 -0
  91. package/data/skills/bio-crispr-screens-screen-qc/SKILL.md +243 -0
  92. package/data/skills/bio-ctdna-mutation-detection/SKILL.md +234 -0
  93. package/data/skills/bio-data-visualization-circos-plots/SKILL.md +405 -0
  94. package/data/skills/bio-data-visualization-color-palettes/SKILL.md +244 -0
  95. package/data/skills/bio-data-visualization-genome-browser-tracks/SKILL.md +328 -0
  96. package/data/skills/bio-data-visualization-genome-tracks/SKILL.md +249 -0
  97. package/data/skills/bio-data-visualization-ggplot2-fundamentals/SKILL.md +313 -0
  98. package/data/skills/bio-data-visualization-heatmaps-clustering/SKILL.md +227 -0
  99. package/data/skills/bio-data-visualization-interactive-visualization/SKILL.md +210 -0
  100. package/data/skills/bio-data-visualization-multipanel-figures/SKILL.md +274 -0
  101. package/data/skills/bio-data-visualization-specialized-omics-plots/SKILL.md +251 -0
  102. package/data/skills/bio-data-visualization-upset-plots/SKILL.md +228 -0
  103. package/data/skills/bio-data-visualization-volcano-customization/SKILL.md +233 -0
  104. package/data/skills/bio-de-deseq2-basics/SKILL.md +376 -0
  105. package/data/skills/bio-de-edger-basics/SKILL.md +418 -0
  106. package/data/skills/bio-de-results/SKILL.md +378 -0
  107. package/data/skills/bio-de-visualization/SKILL.md +408 -0
  108. package/data/skills/bio-differential-expression-batch-correction/SKILL.md +253 -0
  109. package/data/skills/bio-differential-expression-timeseries-de/SKILL.md +370 -0
  110. package/data/skills/bio-differential-splicing/SKILL.md +177 -0
  111. package/data/skills/bio-duplicate-handling/SKILL.md +292 -0
  112. package/data/skills/bio-entrez-fetch/SKILL.md +334 -0
  113. package/data/skills/bio-entrez-link/SKILL.md +325 -0
  114. package/data/skills/bio-entrez-search/SKILL.md +311 -0
  115. package/data/skills/bio-epidemiological-genomics-amr-surveillance/SKILL.md +233 -0
  116. package/data/skills/bio-epidemiological-genomics-pathogen-typing/SKILL.md +202 -0
  117. package/data/skills/bio-epidemiological-genomics-phylodynamics/SKILL.md +207 -0
  118. package/data/skills/bio-epidemiological-genomics-transmission-inference/SKILL.md +237 -0
  119. package/data/skills/bio-epidemiological-genomics-variant-surveillance/SKILL.md +237 -0
  120. package/data/skills/bio-epitranscriptomics-m6a-differential/SKILL.md +88 -0
  121. package/data/skills/bio-epitranscriptomics-m6a-peak-calling/SKILL.md +89 -0
  122. package/data/skills/bio-epitranscriptomics-m6anet-analysis/SKILL.md +101 -0
  123. package/data/skills/bio-epitranscriptomics-merip-preprocessing/SKILL.md +81 -0
  124. package/data/skills/bio-epitranscriptomics-modification-visualization/SKILL.md +98 -0
  125. package/data/skills/bio-experimental-design-batch-design/SKILL.md +110 -0
  126. package/data/skills/bio-experimental-design-multiple-testing/SKILL.md +98 -0
  127. package/data/skills/bio-experimental-design-power-analysis/SKILL.md +84 -0
  128. package/data/skills/bio-experimental-design-sample-size/SKILL.md +93 -0
  129. package/data/skills/bio-expression-matrix-counts-ingest/SKILL.md +220 -0
  130. package/data/skills/bio-expression-matrix-gene-id-mapping/SKILL.md +256 -0
  131. package/data/skills/bio-expression-matrix-metadata-joins/SKILL.md +271 -0
  132. package/data/skills/bio-expression-matrix-sparse-handling/SKILL.md +247 -0
  133. package/data/skills/bio-fastq-quality/SKILL.md +279 -0
  134. package/data/skills/bio-filter-sequences/SKILL.md +265 -0
  135. package/data/skills/bio-flow-cytometry-bead-normalization/SKILL.md +315 -0
  136. package/data/skills/bio-flow-cytometry-clustering-phenotyping/SKILL.md +237 -0
  137. package/data/skills/bio-flow-cytometry-compensation-transformation/SKILL.md +196 -0
  138. package/data/skills/bio-flow-cytometry-cytometry-qc/SKILL.md +382 -0
  139. package/data/skills/bio-flow-cytometry-differential-analysis/SKILL.md +217 -0
  140. package/data/skills/bio-flow-cytometry-doublet-detection/SKILL.md +288 -0
  141. package/data/skills/bio-flow-cytometry-fcs-handling/SKILL.md +221 -0
  142. package/data/skills/bio-flow-cytometry-gating-analysis/SKILL.md +193 -0
  143. package/data/skills/bio-format-conversion/SKILL.md +193 -0
  144. package/data/skills/bio-fragment-analysis/SKILL.md +214 -0
  145. package/data/skills/bio-gatk-variant-calling/SKILL.md +422 -0
  146. package/data/skills/bio-genome-assembly-assembly-polishing/SKILL.md +333 -0
  147. package/data/skills/bio-genome-assembly-assembly-qc/SKILL.md +344 -0
  148. package/data/skills/bio-genome-assembly-contamination-detection/SKILL.md +235 -0
  149. package/data/skills/bio-genome-assembly-hifi-assembly/SKILL.md +178 -0
  150. package/data/skills/bio-genome-assembly-long-read-assembly/SKILL.md +307 -0
  151. package/data/skills/bio-genome-assembly-metagenome-assembly/SKILL.md +227 -0
  152. package/data/skills/bio-genome-assembly-scaffolding/SKILL.md +204 -0
  153. package/data/skills/bio-genome-assembly-short-read-assembly/SKILL.md +319 -0
  154. package/data/skills/bio-genome-engineering-base-editing-design/SKILL.md +277 -0
  155. package/data/skills/bio-genome-engineering-grna-design/SKILL.md +221 -0
  156. package/data/skills/bio-genome-engineering-hdr-template-design/SKILL.md +264 -0
  157. package/data/skills/bio-genome-engineering-off-target-prediction/SKILL.md +232 -0
  158. package/data/skills/bio-genome-engineering-prime-editing-design/SKILL.md +275 -0
  159. package/data/skills/bio-genome-intervals-bed-file-basics/SKILL.md +357 -0
  160. package/data/skills/bio-genome-intervals-bigwig-tracks/SKILL.md +351 -0
  161. package/data/skills/bio-genome-intervals-coverage-analysis/SKILL.md +300 -0
  162. package/data/skills/bio-genome-intervals-gtf-gff-handling/SKILL.md +345 -0
  163. package/data/skills/bio-genome-intervals-interval-arithmetic/SKILL.md +485 -0
  164. package/data/skills/bio-genome-intervals-proximity-operations/SKILL.md +337 -0
  165. package/data/skills/bio-geo-data/SKILL.md +380 -0
  166. package/data/skills/bio-hi-c-analysis-compartment-analysis/SKILL.md +261 -0
  167. package/data/skills/bio-hi-c-analysis-contact-pairs/SKILL.md +278 -0
  168. package/data/skills/bio-hi-c-analysis-hic-data-io/SKILL.md +260 -0
  169. package/data/skills/bio-hi-c-analysis-hic-differential/SKILL.md +328 -0
  170. package/data/skills/bio-hi-c-analysis-hic-visualization/SKILL.md +297 -0
  171. package/data/skills/bio-hi-c-analysis-loop-calling/SKILL.md +284 -0
  172. package/data/skills/bio-hi-c-analysis-matrix-operations/SKILL.md +274 -0
  173. package/data/skills/bio-hi-c-analysis-tad-detection/SKILL.md +239 -0
  174. package/data/skills/bio-imaging-mass-cytometry-cell-segmentation/SKILL.md +241 -0
  175. package/data/skills/bio-imaging-mass-cytometry-data-preprocessing/SKILL.md +279 -0
  176. package/data/skills/bio-imaging-mass-cytometry-interactive-annotation/SKILL.md +304 -0
  177. package/data/skills/bio-imaging-mass-cytometry-phenotyping/SKILL.md +231 -0
  178. package/data/skills/bio-imaging-mass-cytometry-quality-metrics/SKILL.md +316 -0
  179. package/data/skills/bio-imaging-mass-cytometry-spatial-analysis/SKILL.md +246 -0
  180. package/data/skills/bio-immunoinformatics-epitope-prediction/SKILL.md +259 -0
  181. package/data/skills/bio-immunoinformatics-immunogenicity-scoring/SKILL.md +275 -0
  182. package/data/skills/bio-immunoinformatics-mhc-binding-prediction/SKILL.md +260 -0
  183. package/data/skills/bio-immunoinformatics-neoantigen-prediction/SKILL.md +277 -0
  184. package/data/skills/bio-immunoinformatics-tcr-epitope-binding/SKILL.md +257 -0
  185. package/data/skills/bio-isoform-switching/SKILL.md +192 -0
  186. package/data/skills/bio-liquid-biopsy-pipeline/SKILL.md +311 -0
  187. package/data/skills/bio-local-blast/SKILL.md +350 -0
  188. package/data/skills/bio-long-read-sequencing-clair3-variants/SKILL.md +252 -0
  189. package/data/skills/bio-long-read-sequencing-isoseq-analysis/SKILL.md +334 -0
  190. package/data/skills/bio-long-read-sequencing-nanopore-methylation/SKILL.md +110 -0
  191. package/data/skills/bio-longitudinal-monitoring/SKILL.md +271 -0
  192. package/data/skills/bio-longread-alignment/SKILL.md +193 -0
  193. package/data/skills/bio-longread-medaka/SKILL.md +176 -0
  194. package/data/skills/bio-longread-qc/SKILL.md +224 -0
  195. package/data/skills/bio-longread-structural-variants/SKILL.md +201 -0
  196. package/data/skills/bio-machine-learning-atlas-mapping/SKILL.md +139 -0
  197. package/data/skills/bio-machine-learning-biomarker-discovery/SKILL.md +157 -0
  198. package/data/skills/bio-machine-learning-model-validation/SKILL.md +148 -0
  199. package/data/skills/bio-machine-learning-omics-classifiers/SKILL.md +146 -0
  200. package/data/skills/bio-machine-learning-prediction-explanation/SKILL.md +162 -0
  201. package/data/skills/bio-machine-learning-survival-analysis/SKILL.md +176 -0
  202. package/data/skills/bio-metabolomics-lipidomics/SKILL.md +265 -0
  203. package/data/skills/bio-metabolomics-metabolite-annotation/SKILL.md +241 -0
  204. package/data/skills/bio-metabolomics-msdial-preprocessing/SKILL.md +308 -0
  205. package/data/skills/bio-metabolomics-normalization-qc/SKILL.md +283 -0
  206. package/data/skills/bio-metabolomics-pathway-mapping/SKILL.md +237 -0
  207. package/data/skills/bio-metabolomics-statistical-analysis/SKILL.md +276 -0
  208. package/data/skills/bio-metabolomics-targeted-analysis/SKILL.md +314 -0
  209. package/data/skills/bio-metabolomics-xcms-preprocessing/SKILL.md +268 -0
  210. package/data/skills/bio-metagenomics-abundance/SKILL.md +203 -0
  211. package/data/skills/bio-metagenomics-amr-detection/SKILL.md +293 -0
  212. package/data/skills/bio-metagenomics-functional-profiling/SKILL.md +252 -0
  213. package/data/skills/bio-metagenomics-kraken/SKILL.md +204 -0
  214. package/data/skills/bio-metagenomics-metaphlan/SKILL.md +214 -0
  215. package/data/skills/bio-metagenomics-strain-tracking/SKILL.md +292 -0
  216. package/data/skills/bio-metagenomics-visualization/SKILL.md +240 -0
  217. package/data/skills/bio-methylation-based-detection/SKILL.md +223 -0
  218. package/data/skills/bio-methylation-bismark-alignment/SKILL.md +195 -0
  219. package/data/skills/bio-methylation-calling/SKILL.md +200 -0
  220. package/data/skills/bio-methylation-dmr-detection/SKILL.md +211 -0
  221. package/data/skills/bio-methylation-methylkit/SKILL.md +219 -0
  222. package/data/skills/bio-microbiome-amplicon-processing/SKILL.md +137 -0
  223. package/data/skills/bio-microbiome-differential-abundance/SKILL.md +147 -0
  224. package/data/skills/bio-microbiome-diversity-analysis/SKILL.md +188 -0
  225. package/data/skills/bio-microbiome-functional-prediction/SKILL.md +153 -0
  226. package/data/skills/bio-microbiome-qiime2-workflow/SKILL.md +219 -0
  227. package/data/skills/bio-microbiome-taxonomy-assignment/SKILL.md +168 -0
  228. package/data/skills/bio-molecular-descriptors/SKILL.md +200 -0
  229. package/data/skills/bio-molecular-io/SKILL.md +188 -0
  230. package/data/skills/bio-motif-search/SKILL.md +354 -0
  231. package/data/skills/bio-multi-omics-data-harmonization/SKILL.md +228 -0
  232. package/data/skills/bio-multi-omics-mixomics-analysis/SKILL.md +221 -0
  233. package/data/skills/bio-multi-omics-mofa-integration/SKILL.md +225 -0
  234. package/data/skills/bio-multi-omics-similarity-network/SKILL.md +235 -0
  235. package/data/skills/bio-orchestrator/SKILL.md +133 -0
  236. package/data/skills/bio-paired-end-fastq/SKILL.md +334 -0
  237. package/data/skills/bio-pathway-enrichment-visualization/SKILL.md +278 -0
  238. package/data/skills/bio-pathway-go-enrichment/SKILL.md +218 -0
  239. package/data/skills/bio-pathway-gsea/SKILL.md +227 -0
  240. package/data/skills/bio-pathway-kegg-pathways/SKILL.md +234 -0
  241. package/data/skills/bio-pathway-reactome/SKILL.md +215 -0
  242. package/data/skills/bio-pathway-wikipathways/SKILL.md +255 -0
  243. package/data/skills/bio-pdb-geometric-analysis/SKILL.md +475 -0
  244. package/data/skills/bio-pdb-structure-io/SKILL.md +296 -0
  245. package/data/skills/bio-pdb-structure-modification/SKILL.md +448 -0
  246. package/data/skills/bio-pdb-structure-navigation/SKILL.md +335 -0
  247. package/data/skills/bio-phasing-imputation-genotype-imputation/SKILL.md +201 -0
  248. package/data/skills/bio-phasing-imputation-haplotype-phasing/SKILL.md +190 -0
  249. package/data/skills/bio-phasing-imputation-imputation-qc/SKILL.md +265 -0
  250. package/data/skills/bio-phasing-imputation-reference-panels/SKILL.md +203 -0
  251. package/data/skills/bio-phylo-distance-calculations/SKILL.md +307 -0
  252. package/data/skills/bio-phylo-modern-tree-inference/SKILL.md +274 -0
  253. package/data/skills/bio-phylo-tree-io/SKILL.md +252 -0
  254. package/data/skills/bio-phylo-tree-manipulation/SKILL.md +375 -0
  255. package/data/skills/bio-phylo-tree-visualization/SKILL.md +275 -0
  256. package/data/skills/bio-pileup-generation/SKILL.md +314 -0
  257. package/data/skills/bio-population-genetics-association-testing/SKILL.md +293 -0
  258. package/data/skills/bio-population-genetics-linkage-disequilibrium/SKILL.md +260 -0
  259. package/data/skills/bio-population-genetics-plink-basics/SKILL.md +338 -0
  260. package/data/skills/bio-population-genetics-population-structure/SKILL.md +352 -0
  261. package/data/skills/bio-population-genetics-scikit-allel-analysis/SKILL.md +306 -0
  262. package/data/skills/bio-population-genetics-selection-statistics/SKILL.md +251 -0
  263. package/data/skills/bio-primer-design-primer-basics/SKILL.md +289 -0
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@@ -0,0 +1,485 @@
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+ ---
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+ name: pyhealth
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+ description: Comprehensive healthcare AI toolkit for developing, testing, and deploying machine learning models with clinical data. This skill should be used when working with electronic health records (EHR), clinical prediction tasks (mortality, readmission, drug recommendation), medical coding systems (ICD, NDC, ATC), physiological signals (EEG, ECG), healthcare datasets (MIMIC-III/IV, eICU, OMOP), or implementing deep learning models for healthcare applications (RETAIN, SafeDrug, Transformer, GNN).
4
+ ---
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+
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+ # PyHealth: Healthcare AI Toolkit
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+
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+ ## Overview
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+
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+ PyHealth is a comprehensive Python library for healthcare AI that provides specialized tools, models, and datasets for clinical machine learning. Use this skill when developing healthcare prediction models, processing clinical data, working with medical coding systems, or deploying AI solutions in healthcare settings.
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+
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+ ## When to Use This Skill
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+
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+ Invoke this skill when:
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+
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+ - **Working with healthcare datasets**: MIMIC-III, MIMIC-IV, eICU, OMOP, sleep EEG data, medical images
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+ - **Clinical prediction tasks**: Mortality prediction, hospital readmission, length of stay, drug recommendation
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+ - **Medical coding**: Translating between ICD-9/10, NDC, RxNorm, ATC coding systems
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+ - **Processing clinical data**: Sequential events, physiological signals, clinical text, medical images
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+ - **Implementing healthcare models**: RETAIN, SafeDrug, GAMENet, StageNet, Transformer for EHR
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+ - **Evaluating clinical models**: Fairness metrics, calibration, interpretability, uncertainty quantification
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+
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+ ## Core Capabilities
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+
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+ PyHealth operates through a modular 5-stage pipeline optimized for healthcare AI:
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+
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+ 1. **Data Loading**: Access 10+ healthcare datasets with standardized interfaces
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+ 2. **Task Definition**: Apply 20+ predefined clinical prediction tasks or create custom tasks
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+ 3. **Model Selection**: Choose from 33+ models (baselines, deep learning, healthcare-specific)
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+ 4. **Training**: Train with automatic checkpointing, monitoring, and evaluation
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+ 5. **Deployment**: Calibrate, interpret, and validate for clinical use
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+
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+ **Performance**: 3x faster than pandas for healthcare data processing
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+
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+ ## Quick Start Workflow
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+
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+ ```python
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+ from pyhealth.datasets import MIMIC4Dataset
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+ from pyhealth.tasks import mortality_prediction_mimic4_fn
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+ from pyhealth.datasets import split_by_patient, get_dataloader
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+ from pyhealth.models import Transformer
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+ from pyhealth.trainer import Trainer
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+
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+ # 1. Load dataset and set task
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+ dataset = MIMIC4Dataset(root="/path/to/data")
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+ sample_dataset = dataset.set_task(mortality_prediction_mimic4_fn)
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+
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+ # 2. Split data
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+ train, val, test = split_by_patient(sample_dataset, [0.7, 0.1, 0.2])
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+
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+ # 3. Create data loaders
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+ train_loader = get_dataloader(train, batch_size=64, shuffle=True)
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+ val_loader = get_dataloader(val, batch_size=64, shuffle=False)
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+ test_loader = get_dataloader(test, batch_size=64, shuffle=False)
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+
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+ # 4. Initialize and train model
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+ model = Transformer(
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+ dataset=sample_dataset,
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+ feature_keys=["diagnoses", "medications"],
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+ mode="binary",
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+ embedding_dim=128
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+ )
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+
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+ trainer = Trainer(model=model, device="cuda")
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+ trainer.train(
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+ train_dataloader=train_loader,
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+ val_dataloader=val_loader,
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+ epochs=50,
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+ monitor="pr_auc_score"
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+ )
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+
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+ # 5. Evaluate
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+ results = trainer.evaluate(test_loader)
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+ ```
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+
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+ ## Detailed Documentation
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+
78
+ This skill includes comprehensive reference documentation organized by functionality. Read specific reference files as needed:
79
+
80
+ ### 1. Datasets and Data Structures
81
+
82
+ **File**: `references/datasets.md`
83
+
84
+ **Read when:**
85
+ - Loading healthcare datasets (MIMIC, eICU, OMOP, sleep EEG, etc.)
86
+ - Understanding Event, Patient, Visit data structures
87
+ - Processing different data types (EHR, signals, images, text)
88
+ - Splitting data for training/validation/testing
89
+ - Working with SampleDataset for task-specific formatting
90
+
91
+ **Key Topics:**
92
+ - Core data structures (Event, Patient, Visit)
93
+ - 10+ available datasets (EHR, physiological signals, imaging, text)
94
+ - Data loading and iteration
95
+ - Train/val/test splitting strategies
96
+ - Performance optimization for large datasets
97
+
98
+ ### 2. Medical Coding Translation
99
+
100
+ **File**: `references/medical_coding.md`
101
+
102
+ **Read when:**
103
+ - Translating between medical coding systems
104
+ - Working with diagnosis codes (ICD-9-CM, ICD-10-CM, CCS)
105
+ - Processing medication codes (NDC, RxNorm, ATC)
106
+ - Standardizing procedure codes (ICD-9-PROC, ICD-10-PROC)
107
+ - Grouping codes into clinical categories
108
+ - Handling hierarchical drug classifications
109
+
110
+ **Key Topics:**
111
+ - InnerMap for within-system lookups
112
+ - CrossMap for cross-system translation
113
+ - Supported coding systems (ICD, NDC, ATC, CCS, RxNorm)
114
+ - Code standardization and hierarchy traversal
115
+ - Medication classification by therapeutic class
116
+ - Integration with datasets
117
+
118
+ ### 3. Clinical Prediction Tasks
119
+
120
+ **File**: `references/tasks.md`
121
+
122
+ **Read when:**
123
+ - Defining clinical prediction objectives
124
+ - Using predefined tasks (mortality, readmission, drug recommendation)
125
+ - Working with EHR, signal, imaging, or text-based tasks
126
+ - Creating custom prediction tasks
127
+ - Setting up input/output schemas for models
128
+ - Applying task-specific filtering logic
129
+
130
+ **Key Topics:**
131
+ - 20+ predefined clinical tasks
132
+ - EHR tasks (mortality, readmission, length of stay, drug recommendation)
133
+ - Signal tasks (sleep staging, EEG analysis, seizure detection)
134
+ - Imaging tasks (COVID-19 chest X-ray classification)
135
+ - Text tasks (medical coding, specialty classification)
136
+ - Custom task creation patterns
137
+
138
+ ### 4. Models and Architectures
139
+
140
+ **File**: `references/models.md`
141
+
142
+ **Read when:**
143
+ - Selecting models for clinical prediction
144
+ - Understanding model architectures and capabilities
145
+ - Choosing between general-purpose and healthcare-specific models
146
+ - Implementing interpretable models (RETAIN, AdaCare)
147
+ - Working with medication recommendation (SafeDrug, GAMENet)
148
+ - Using graph neural networks for healthcare
149
+ - Configuring model hyperparameters
150
+
151
+ **Key Topics:**
152
+ - 33+ available models
153
+ - General-purpose: Logistic Regression, MLP, CNN, RNN, Transformer, GNN
154
+ - Healthcare-specific: RETAIN, SafeDrug, GAMENet, StageNet, AdaCare
155
+ - Model selection by task type and data type
156
+ - Interpretability considerations
157
+ - Computational requirements
158
+ - Hyperparameter tuning guidelines
159
+
160
+ ### 5. Data Preprocessing
161
+
162
+ **File**: `references/preprocessing.md`
163
+
164
+ **Read when:**
165
+ - Preprocessing clinical data for models
166
+ - Handling sequential events and time-series data
167
+ - Processing physiological signals (EEG, ECG)
168
+ - Normalizing lab values and vital signs
169
+ - Preparing labels for different task types
170
+ - Building feature vocabularies
171
+ - Managing missing data and outliers
172
+
173
+ **Key Topics:**
174
+ - 15+ processor types
175
+ - Sequence processing (padding, truncation)
176
+ - Signal processing (filtering, segmentation)
177
+ - Feature extraction and encoding
178
+ - Label processors (binary, multi-class, multi-label, regression)
179
+ - Text and image preprocessing
180
+ - Common preprocessing workflows
181
+
182
+ ### 6. Training and Evaluation
183
+
184
+ **File**: `references/training_evaluation.md`
185
+
186
+ **Read when:**
187
+ - Training models with the Trainer class
188
+ - Evaluating model performance
189
+ - Computing clinical metrics
190
+ - Assessing model fairness across demographics
191
+ - Calibrating predictions for reliability
192
+ - Quantifying prediction uncertainty
193
+ - Interpreting model predictions
194
+ - Preparing models for clinical deployment
195
+
196
+ **Key Topics:**
197
+ - Trainer class (train, evaluate, inference)
198
+ - Metrics for binary, multi-class, multi-label, regression tasks
199
+ - Fairness metrics for bias assessment
200
+ - Calibration methods (Platt scaling, temperature scaling)
201
+ - Uncertainty quantification (conformal prediction, MC dropout)
202
+ - Interpretability tools (attention visualization, SHAP, ChEFER)
203
+ - Complete training pipeline example
204
+
205
+ ## Installation
206
+
207
+ ```bash
208
+ uv pip install pyhealth
209
+ ```
210
+
211
+ **Requirements:**
212
+ - Python ≥ 3.7
213
+ - PyTorch ≥ 1.8
214
+ - NumPy, pandas, scikit-learn
215
+
216
+ ## Common Use Cases
217
+
218
+ ### Use Case 1: ICU Mortality Prediction
219
+
220
+ **Objective**: Predict patient mortality in intensive care unit
221
+
222
+ **Approach:**
223
+ 1. Load MIMIC-IV dataset → Read `references/datasets.md`
224
+ 2. Apply mortality prediction task → Read `references/tasks.md`
225
+ 3. Select interpretable model (RETAIN) → Read `references/models.md`
226
+ 4. Train and evaluate → Read `references/training_evaluation.md`
227
+ 5. Interpret predictions for clinical use → Read `references/training_evaluation.md`
228
+
229
+ ### Use Case 2: Safe Medication Recommendation
230
+
231
+ **Objective**: Recommend medications while avoiding drug-drug interactions
232
+
233
+ **Approach:**
234
+ 1. Load EHR dataset (MIMIC-IV or OMOP) → Read `references/datasets.md`
235
+ 2. Apply drug recommendation task → Read `references/tasks.md`
236
+ 3. Use SafeDrug model with DDI constraints → Read `references/models.md`
237
+ 4. Preprocess medication codes → Read `references/medical_coding.md`
238
+ 5. Evaluate with multi-label metrics → Read `references/training_evaluation.md`
239
+
240
+ ### Use Case 3: Hospital Readmission Prediction
241
+
242
+ **Objective**: Identify patients at risk of 30-day readmission
243
+
244
+ **Approach:**
245
+ 1. Load multi-site EHR data (eICU or OMOP) → Read `references/datasets.md`
246
+ 2. Apply readmission prediction task → Read `references/tasks.md`
247
+ 3. Handle class imbalance in preprocessing → Read `references/preprocessing.md`
248
+ 4. Train Transformer model → Read `references/models.md`
249
+ 5. Calibrate predictions and assess fairness → Read `references/training_evaluation.md`
250
+
251
+ ### Use Case 4: Sleep Disorder Diagnosis
252
+
253
+ **Objective**: Classify sleep stages from EEG signals
254
+
255
+ **Approach:**
256
+ 1. Load sleep EEG dataset (SleepEDF, SHHS) → Read `references/datasets.md`
257
+ 2. Apply sleep staging task → Read `references/tasks.md`
258
+ 3. Preprocess EEG signals (filtering, segmentation) → Read `references/preprocessing.md`
259
+ 4. Train CNN or RNN model → Read `references/models.md`
260
+ 5. Evaluate per-stage performance → Read `references/training_evaluation.md`
261
+
262
+ ### Use Case 5: Medical Code Translation
263
+
264
+ **Objective**: Standardize diagnoses across different coding systems
265
+
266
+ **Approach:**
267
+ 1. Read `references/medical_coding.md` for comprehensive guidance
268
+ 2. Use CrossMap to translate between ICD-9, ICD-10, CCS
269
+ 3. Group codes into clinically meaningful categories
270
+ 4. Integrate with dataset processing
271
+
272
+ ### Use Case 6: Clinical Text to ICD Coding
273
+
274
+ **Objective**: Automatically assign ICD codes from clinical notes
275
+
276
+ **Approach:**
277
+ 1. Load MIMIC-III with clinical text → Read `references/datasets.md`
278
+ 2. Apply ICD coding task → Read `references/tasks.md`
279
+ 3. Preprocess clinical text → Read `references/preprocessing.md`
280
+ 4. Use TransformersModel (ClinicalBERT) → Read `references/models.md`
281
+ 5. Evaluate with multi-label metrics → Read `references/training_evaluation.md`
282
+
283
+ ## Best Practices
284
+
285
+ ### Data Handling
286
+
287
+ 1. **Always split by patient**: Prevent data leakage by ensuring no patient appears in multiple splits
288
+ ```python
289
+ from pyhealth.datasets import split_by_patient
290
+ train, val, test = split_by_patient(dataset, [0.7, 0.1, 0.2])
291
+ ```
292
+
293
+ 2. **Check dataset statistics**: Understand your data before modeling
294
+ ```python
295
+ print(dataset.stats()) # Patients, visits, events, code distributions
296
+ ```
297
+
298
+ 3. **Use appropriate preprocessing**: Match processors to data types (see `references/preprocessing.md`)
299
+
300
+ ### Model Development
301
+
302
+ 1. **Start with baselines**: Establish baseline performance with simple models
303
+ - Logistic Regression for binary/multi-class tasks
304
+ - MLP for initial deep learning baseline
305
+
306
+ 2. **Choose task-appropriate models**:
307
+ - Interpretability needed → RETAIN, AdaCare
308
+ - Drug recommendation → SafeDrug, GAMENet
309
+ - Long sequences → Transformer
310
+ - Graph relationships → GNN
311
+
312
+ 3. **Monitor validation metrics**: Use appropriate metrics for task and handle class imbalance
313
+ - Binary classification: AUROC, AUPRC (especially for rare events)
314
+ - Multi-class: macro-F1 (for imbalanced), weighted-F1
315
+ - Multi-label: Jaccard, example-F1
316
+ - Regression: MAE, RMSE
317
+
318
+ ### Clinical Deployment
319
+
320
+ 1. **Calibrate predictions**: Ensure probabilities are reliable (see `references/training_evaluation.md`)
321
+
322
+ 2. **Assess fairness**: Evaluate across demographic groups to detect bias
323
+
324
+ 3. **Quantify uncertainty**: Provide confidence estimates for predictions
325
+
326
+ 4. **Interpret predictions**: Use attention weights, SHAP, or ChEFER for clinical trust
327
+
328
+ 5. **Validate thoroughly**: Use held-out test sets from different time periods or sites
329
+
330
+ ## Limitations and Considerations
331
+
332
+ ### Data Requirements
333
+
334
+ - **Large datasets**: Deep learning models require sufficient data (thousands of patients)
335
+ - **Data quality**: Missing data and coding errors impact performance
336
+ - **Temporal consistency**: Ensure train/test split respects temporal ordering when needed
337
+
338
+ ### Clinical Validation
339
+
340
+ - **External validation**: Test on data from different hospitals/systems
341
+ - **Prospective evaluation**: Validate in real clinical settings before deployment
342
+ - **Clinical review**: Have clinicians review predictions and interpretations
343
+ - **Ethical considerations**: Address privacy (HIPAA/GDPR), fairness, and safety
344
+
345
+ ### Computational Resources
346
+
347
+ - **GPU recommended**: For training deep learning models efficiently
348
+ - **Memory requirements**: Large datasets may require 16GB+ RAM
349
+ - **Storage**: Healthcare datasets can be 10s-100s of GB
350
+
351
+ ## Troubleshooting
352
+
353
+ ### Common Issues
354
+
355
+ **ImportError for dataset**:
356
+ - Ensure dataset files are downloaded and path is correct
357
+ - Check PyHealth version compatibility
358
+
359
+ **Out of memory**:
360
+ - Reduce batch size
361
+ - Reduce sequence length (`max_seq_length`)
362
+ - Use gradient accumulation
363
+ - Process data in chunks
364
+
365
+ **Poor performance**:
366
+ - Check class imbalance and use appropriate metrics (AUPRC vs AUROC)
367
+ - Verify preprocessing (normalization, missing data handling)
368
+ - Increase model capacity or training epochs
369
+ - Check for data leakage in train/test split
370
+
371
+ **Slow training**:
372
+ - Use GPU (`device="cuda"`)
373
+ - Increase batch size (if memory allows)
374
+ - Reduce sequence length
375
+ - Use more efficient model (CNN vs Transformer)
376
+
377
+ ### Getting Help
378
+
379
+ - **Documentation**: https://pyhealth.readthedocs.io/
380
+ - **GitHub Issues**: https://github.com/sunlabuiuc/PyHealth/issues
381
+ - **Tutorials**: 7 core tutorials + 5 practical pipelines available online
382
+
383
+ ## Example: Complete Workflow
384
+
385
+ ```python
386
+ # Complete mortality prediction pipeline
387
+ from pyhealth.datasets import MIMIC4Dataset
388
+ from pyhealth.tasks import mortality_prediction_mimic4_fn
389
+ from pyhealth.datasets import split_by_patient, get_dataloader
390
+ from pyhealth.models import RETAIN
391
+ from pyhealth.trainer import Trainer
392
+
393
+ # 1. Load dataset
394
+ print("Loading MIMIC-IV dataset...")
395
+ dataset = MIMIC4Dataset(root="/data/mimic4")
396
+ print(dataset.stats())
397
+
398
+ # 2. Define task
399
+ print("Setting mortality prediction task...")
400
+ sample_dataset = dataset.set_task(mortality_prediction_mimic4_fn)
401
+ print(f"Generated {len(sample_dataset)} samples")
402
+
403
+ # 3. Split data (by patient to prevent leakage)
404
+ print("Splitting data...")
405
+ train_ds, val_ds, test_ds = split_by_patient(
406
+ sample_dataset, ratios=[0.7, 0.1, 0.2], seed=42
407
+ )
408
+
409
+ # 4. Create data loaders
410
+ train_loader = get_dataloader(train_ds, batch_size=64, shuffle=True)
411
+ val_loader = get_dataloader(val_ds, batch_size=64)
412
+ test_loader = get_dataloader(test_ds, batch_size=64)
413
+
414
+ # 5. Initialize interpretable model
415
+ print("Initializing RETAIN model...")
416
+ model = RETAIN(
417
+ dataset=sample_dataset,
418
+ feature_keys=["diagnoses", "procedures", "medications"],
419
+ mode="binary",
420
+ embedding_dim=128,
421
+ hidden_dim=128
422
+ )
423
+
424
+ # 6. Train model
425
+ print("Training model...")
426
+ trainer = Trainer(model=model, device="cuda")
427
+ trainer.train(
428
+ train_dataloader=train_loader,
429
+ val_dataloader=val_loader,
430
+ epochs=50,
431
+ optimizer="Adam",
432
+ learning_rate=1e-3,
433
+ weight_decay=1e-5,
434
+ monitor="pr_auc_score", # Use AUPRC for imbalanced data
435
+ monitor_criterion="max",
436
+ save_path="./checkpoints/mortality_retain"
437
+ )
438
+
439
+ # 7. Evaluate on test set
440
+ print("Evaluating on test set...")
441
+ test_results = trainer.evaluate(
442
+ test_loader,
443
+ metrics=["accuracy", "precision", "recall", "f1_score",
444
+ "roc_auc_score", "pr_auc_score"]
445
+ )
446
+
447
+ print("\nTest Results:")
448
+ for metric, value in test_results.items():
449
+ print(f" {metric}: {value:.4f}")
450
+
451
+ # 8. Get predictions with attention for interpretation
452
+ predictions = trainer.inference(
453
+ test_loader,
454
+ additional_outputs=["visit_attention", "feature_attention"],
455
+ return_patient_ids=True
456
+ )
457
+
458
+ # 9. Analyze a high-risk patient
459
+ high_risk_idx = predictions["y_pred"].argmax()
460
+ patient_id = predictions["patient_ids"][high_risk_idx]
461
+ visit_attn = predictions["visit_attention"][high_risk_idx]
462
+ feature_attn = predictions["feature_attention"][high_risk_idx]
463
+
464
+ print(f"\nHigh-risk patient: {patient_id}")
465
+ print(f"Risk score: {predictions['y_pred'][high_risk_idx]:.3f}")
466
+ print(f"Most influential visit: {visit_attn.argmax()}")
467
+ print(f"Most important features: {feature_attn[visit_attn.argmax()].argsort()[-5:]}")
468
+
469
+ # 10. Save model for deployment
470
+ trainer.save("./models/mortality_retain_final.pt")
471
+ print("\nModel saved successfully!")
472
+ ```
473
+
474
+ ## Resources
475
+
476
+ For detailed information on each component, refer to the comprehensive reference files in the `references/` directory:
477
+
478
+ - **datasets.md**: Data structures, loading, and splitting (4,500 words)
479
+ - **medical_coding.md**: Code translation and standardization (3,800 words)
480
+ - **tasks.md**: Clinical prediction tasks and custom task creation (4,200 words)
481
+ - **models.md**: Model architectures and selection guidelines (5,100 words)
482
+ - **preprocessing.md**: Data processors and preprocessing workflows (4,600 words)
483
+ - **training_evaluation.md**: Training, metrics, calibration, interpretability (5,900 words)
484
+
485
+ **Total comprehensive documentation**: ~28,000 words across modular reference files.
@@ -0,0 +1,179 @@
1
+ ---
2
+ name: pylabrobot
3
+ description: Laboratory automation toolkit for controlling liquid handlers, plate readers, pumps, heater shakers, incubators, centrifuges, and analytical equipment. Use this skill when automating laboratory workflows, programming liquid handling robots (Hamilton STAR, Opentrons OT-2, Tecan EVO), integrating lab equipment, managing deck layouts and resources (plates, tips, containers), reading plates, or creating reproducible laboratory protocols. Applicable for both simulated protocols and physical hardware control.
4
+ ---
5
+
6
+ # PyLabRobot
7
+
8
+ ## Overview
9
+
10
+ PyLabRobot is a hardware-agnostic, pure Python Software Development Kit for automated and autonomous laboratories. Use this skill to control liquid handling robots, plate readers, pumps, heater shakers, incubators, centrifuges, and other laboratory automation equipment through a unified Python interface that works across platforms (Windows, macOS, Linux).
11
+
12
+ ## When to Use This Skill
13
+
14
+ Use this skill when:
15
+ - Programming liquid handling robots (Hamilton STAR/STARlet, Opentrons OT-2, Tecan EVO)
16
+ - Automating laboratory workflows involving pipetting, sample preparation, or analytical measurements
17
+ - Managing deck layouts and laboratory resources (plates, tips, containers, troughs)
18
+ - Integrating multiple lab devices (liquid handlers, plate readers, heater shakers, pumps)
19
+ - Creating reproducible laboratory protocols with state management
20
+ - Simulating protocols before running on physical hardware
21
+ - Reading plates using BMG CLARIOstar or other supported plate readers
22
+ - Controlling temperature, shaking, centrifugation, or other material handling operations
23
+ - Working with laboratory automation in Python
24
+
25
+ ## Core Capabilities
26
+
27
+ PyLabRobot provides comprehensive laboratory automation through six main capability areas, each detailed in the references/ directory:
28
+
29
+ ### 1. Liquid Handling (`references/liquid-handling.md`)
30
+
31
+ Control liquid handling robots for aspirating, dispensing, and transferring liquids. Key operations include:
32
+ - **Basic Operations**: Aspirate, dispense, transfer liquids between wells
33
+ - **Tip Management**: Pick up, drop, and track pipette tips automatically
34
+ - **Advanced Techniques**: Multi-channel pipetting, serial dilutions, plate replication
35
+ - **Volume Tracking**: Automatic tracking of liquid volumes in wells
36
+ - **Hardware Support**: Hamilton STAR/STARlet, Opentrons OT-2, Tecan EVO, and others
37
+
38
+ ### 2. Resource Management (`references/resources.md`)
39
+
40
+ Manage laboratory resources in a hierarchical system:
41
+ - **Resource Types**: Plates, tip racks, troughs, tubes, carriers, and custom labware
42
+ - **Deck Layout**: Assign resources to deck positions with coordinate systems
43
+ - **State Management**: Track tip presence, liquid volumes, and resource states
44
+ - **Serialization**: Save and load deck layouts and states from JSON files
45
+ - **Resource Discovery**: Access wells, tips, and containers through intuitive APIs
46
+
47
+ ### 3. Hardware Backends (`references/hardware-backends.md`)
48
+
49
+ Connect to diverse laboratory equipment through backend abstraction:
50
+ - **Liquid Handlers**: Hamilton STAR (full support), Opentrons OT-2, Tecan EVO
51
+ - **Simulation**: ChatterboxBackend for protocol testing without hardware
52
+ - **Platform Support**: Works on Windows, macOS, Linux, and Raspberry Pi
53
+ - **Backend Switching**: Change robots by swapping backend without rewriting protocols
54
+
55
+ ### 4. Analytical Equipment (`references/analytical-equipment.md`)
56
+
57
+ Integrate plate readers and analytical instruments:
58
+ - **Plate Readers**: BMG CLARIOstar for absorbance, luminescence, fluorescence
59
+ - **Scales**: Mettler Toledo integration for mass measurements
60
+ - **Integration Patterns**: Combine liquid handlers with analytical equipment
61
+ - **Automated Workflows**: Move plates between devices automatically
62
+
63
+ ### 5. Material Handling (`references/material-handling.md`)
64
+
65
+ Control environmental and material handling equipment:
66
+ - **Heater Shakers**: Hamilton HeaterShaker, Inheco ThermoShake
67
+ - **Incubators**: Inheco and Thermo Fisher incubators with temperature control
68
+ - **Centrifuges**: Agilent VSpin with bucket positioning and spin control
69
+ - **Pumps**: Cole Parmer Masterflex for fluid pumping operations
70
+ - **Temperature Control**: Set and monitor temperatures during protocols
71
+
72
+ ### 6. Visualization & Simulation (`references/visualization.md`)
73
+
74
+ Visualize and simulate laboratory protocols:
75
+ - **Browser Visualizer**: Real-time 3D visualization of deck state
76
+ - **Simulation Mode**: Test protocols without physical hardware
77
+ - **State Tracking**: Monitor tip presence and liquid volumes visually
78
+ - **Deck Editor**: Graphical tool for designing deck layouts
79
+ - **Protocol Validation**: Verify protocols before running on hardware
80
+
81
+ ## Quick Start
82
+
83
+ To get started with PyLabRobot, install the package and initialize a liquid handler:
84
+
85
+ ```python
86
+ # Install PyLabRobot
87
+ # uv pip install pylabrobot
88
+
89
+ # Basic liquid handling setup
90
+ from pylabrobot.liquid_handling import LiquidHandler
91
+ from pylabrobot.liquid_handling.backends import STAR
92
+ from pylabrobot.resources import STARLetDeck
93
+
94
+ # Initialize liquid handler
95
+ lh = LiquidHandler(backend=STAR(), deck=STARLetDeck())
96
+ await lh.setup()
97
+
98
+ # Basic operations
99
+ await lh.pick_up_tips(tip_rack["A1:H1"])
100
+ await lh.aspirate(plate["A1"], vols=100)
101
+ await lh.dispense(plate["A2"], vols=100)
102
+ await lh.drop_tips()
103
+ ```
104
+
105
+ ## Working with References
106
+
107
+ This skill organizes detailed information across multiple reference files. Load the relevant reference when:
108
+ - **Liquid Handling**: Writing pipetting protocols, tip management, transfers
109
+ - **Resources**: Defining deck layouts, managing plates/tips, custom labware
110
+ - **Hardware Backends**: Connecting to specific robots, switching platforms
111
+ - **Analytical Equipment**: Integrating plate readers, scales, or analytical devices
112
+ - **Material Handling**: Using heater shakers, incubators, centrifuges, pumps
113
+ - **Visualization**: Simulating protocols, visualizing deck states
114
+
115
+ All reference files can be found in the `references/` directory and contain comprehensive examples, API usage patterns, and best practices.
116
+
117
+ ## Best Practices
118
+
119
+ When creating laboratory automation protocols with PyLabRobot:
120
+
121
+ 1. **Start with Simulation**: Use ChatterboxBackend and the visualizer to test protocols before running on hardware
122
+ 2. **Enable Tracking**: Turn on tip tracking and volume tracking for accurate state management
123
+ 3. **Resource Naming**: Use clear, descriptive names for all resources (plates, tip racks, containers)
124
+ 4. **State Serialization**: Save deck layouts and states to JSON for reproducibility
125
+ 5. **Error Handling**: Implement proper async error handling for hardware operations
126
+ 6. **Temperature Control**: Set temperatures early as heating/cooling takes time
127
+ 7. **Modular Protocols**: Break complex workflows into reusable functions
128
+ 8. **Documentation**: Reference official docs at https://docs.pylabrobot.org for latest features
129
+
130
+ ## Common Workflows
131
+
132
+ ### Liquid Transfer Protocol
133
+
134
+ ```python
135
+ # Setup
136
+ lh = LiquidHandler(backend=STAR(), deck=STARLetDeck())
137
+ await lh.setup()
138
+
139
+ # Define resources
140
+ tip_rack = TIP_CAR_480_A00(name="tip_rack")
141
+ source_plate = Cos_96_DW_1mL(name="source")
142
+ dest_plate = Cos_96_DW_1mL(name="dest")
143
+
144
+ lh.deck.assign_child_resource(tip_rack, rails=1)
145
+ lh.deck.assign_child_resource(source_plate, rails=10)
146
+ lh.deck.assign_child_resource(dest_plate, rails=15)
147
+
148
+ # Transfer protocol
149
+ await lh.pick_up_tips(tip_rack["A1:H1"])
150
+ await lh.transfer(source_plate["A1:H12"], dest_plate["A1:H12"], vols=100)
151
+ await lh.drop_tips()
152
+ ```
153
+
154
+ ### Plate Reading Workflow
155
+
156
+ ```python
157
+ # Setup plate reader
158
+ from pylabrobot.plate_reading import PlateReader
159
+ from pylabrobot.plate_reading.clario_star_backend import CLARIOstarBackend
160
+
161
+ pr = PlateReader(name="CLARIOstar", backend=CLARIOstarBackend())
162
+ await pr.setup()
163
+
164
+ # Set temperature and read
165
+ await pr.set_temperature(37)
166
+ await pr.open()
167
+ # (manually or robotically load plate)
168
+ await pr.close()
169
+ data = await pr.read_absorbance(wavelength=450)
170
+ ```
171
+
172
+ ## Additional Resources
173
+
174
+ - **Official Documentation**: https://docs.pylabrobot.org
175
+ - **GitHub Repository**: https://github.com/PyLabRobot/pylabrobot
176
+ - **Community Forum**: https://discuss.pylabrobot.org
177
+ - **PyPI Package**: https://pypi.org/project/PyLabRobot/
178
+
179
+ For detailed usage of specific capabilities, refer to the corresponding reference file in the `references/` directory.