ngs_server 0.1 → 0.2
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- data/bin/ngs_server +72 -50
- data/ext/bamtools/extconf.rb +3 -3
- data/ext/vcftools/Makefile +28 -0
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<div class="item">
|
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<h3>Documentation</h3>
|
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|
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The VCFtools package is broadly split into two sections: <br>
|
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|
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<li> The <span class="cmd">vcftools</span> binary program, generally used to analyse VCF files.
|
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<li> The <span class="cmd">Vcf.pm</span> perl module, which is a general Perl API containing a core of
|
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the utilities <span class="cmd">vcf-convert</span>, <span class="cmd">vcf-merge</span>,
|
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<span class="cmd">vcf-compare</span>, <span class="cmd">vcf-isec</span>, and others.
|
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|
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</p>
|
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<h3>Documentation</h3>
|
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|
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<li> <a href="options.html">Documentation for the binary executable <span class="cmd">vcftools</span></a>
|
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|
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<li> <a href="perl_module.html">The Perl API and scripts</a>
|
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<li> <a href="#one-liners">Useful shell one-liners</a>
|
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|
+
</ul>
|
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|
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|
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|
+
<h3>Examples of usage by topic</h3>
|
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+
<ul class="faq">
|
24
|
+
<li> <a href="#install">Installation</a>
|
25
|
+
<li> <a href="#annotate">Annotating</a>
|
26
|
+
<li> <a href="#compare">Comparing</a>
|
27
|
+
<li> <a href="#concat">Concatenating</a>
|
28
|
+
<li> <a href="#convert">Converting</a>
|
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|
+
<li> <a href="#isec">Intersections, complements</a>
|
30
|
+
<li> <a href="#merge">Merging</a>
|
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|
+
<li> <a href="#query">Querying</a>
|
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<li> <a href="#shuffle">Reordering columns</a>
|
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<li> <a href="#stats">Stats</a>
|
34
|
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<li> <a href="#subset">Stripping columns</a>
|
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|
+
<li> <a href="#one-liners">Useful shell one-liners</a>
|
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|
+
<li> <a href="#validator">Validating</a>
|
37
|
+
<li> <a href="#more">...and more</a>
|
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|
+
</ul>
|
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|
+
</div>
|
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|
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|
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|
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|
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<div class="item">
|
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+
<a name="install" class="Q">Installation</a>
|
44
|
+
<p> The VCFtools package can be decompressed by the command
|
45
|
+
<p class="codebox">tar -xzf vcftools_<i>version_number</i>_source.tar.gz</p>
|
46
|
+
<p> To build the <span class="cmd">vcftools</span> executable, type "make" in the vcftools folder. </p>
|
47
|
+
<p>
|
48
|
+
The <b>Perl scripts require that VCF files are compressed by <span class="cmd">bgzip</span>
|
49
|
+
and indexed by <span class="cmd">tabix</span></b> (both tools are part of the tabix package,
|
50
|
+
available for <a href="https://sourceforge.net/projects/samtools/files/tabix/">download here</a>).
|
51
|
+
Test the tools can be tested by running the script
|
52
|
+
<p class="codebox">/path/to/your/installation/perl/test.t</p>
|
53
|
+
For running the Perl scripts, the <b>PERL5LIB environment variable must be set</b> to include the Vcf.pm
|
54
|
+
module</p>
|
55
|
+
<p class="codebox">export PERL5LIB=/path/to/your/installation/perl</p>
|
56
|
+
</div>
|
57
|
+
|
58
|
+
|
59
|
+
<div class="item">
|
60
|
+
<a name="annotate" class="Q">Annotating</a>
|
61
|
+
<p></p>
|
62
|
+
<p class="codebox">
|
63
|
+
# Add custom annotations <br>
|
64
|
+
cat in.vcf | vcf-annotate -a annotations.gz \ <br>
|
65
|
+
-d key=INFO,ID=ANN,Number=1,Type=Integer,Description='My custom annotation' \ <br>
|
66
|
+
-c CHROM,FROM,TO,INFO/ANN > out.vcf <br>
|
67
|
+
<br>
|
68
|
+
# Apply SnpCluster filter <br>
|
69
|
+
cat in.vcf | <a href="perl_module.html#vcf-annotate">vcf-annotate</a> --filter SnpCluster=3,10 > out.vcf <br>
|
70
|
+
</p>
|
71
|
+
</div>
|
72
|
+
|
73
|
+
|
74
|
+
|
75
|
+
<div class="item">
|
76
|
+
<a name="compare" class="Q">Comparing</a>
|
77
|
+
<p></p>
|
78
|
+
<p class="codebox"><a href="perl_module.html#vcf-compare">vcf-compare</a> A.vcf.gz B.vcf.gz C.vcf.gz
|
79
|
+
</p>
|
80
|
+
</div>
|
81
|
+
|
82
|
+
|
83
|
+
<div class="item">
|
84
|
+
<a name="concat" class="Q">Concatenating</a>
|
85
|
+
<p></p>
|
86
|
+
<p class="codebox"><a href="perl_module.html#vcf-concat">vcf-concat</a> A.vcf.gz B.vcf.gz C.vcf.gz | bgzip -c > out.vcf.gz
|
87
|
+
</p>
|
88
|
+
</div>
|
89
|
+
|
90
|
+
|
91
|
+
<div class="item">
|
92
|
+
<a name="convert" class="Q">Converting</a>
|
93
|
+
<p></p>
|
94
|
+
<p class="codebox">
|
95
|
+
# Convert between VCF versions <br>
|
96
|
+
zcat file.vcf.gz | <a href="perl_module.html#vcf-convert">vcf-convert</a> -r reference.fa | bgzip -c > out.vcf.gz <br>
|
97
|
+
<br>
|
98
|
+
# Convert from VCF format to tab-delimited text file <br>
|
99
|
+
zcat file.vcf.gz | <a href="perl_module.html#vcf-to-tab">vcf-to-tab</a> > out.tab <br>
|
100
|
+
</p>
|
101
|
+
</div>
|
102
|
+
|
103
|
+
|
104
|
+
<div class="item">
|
105
|
+
<a name="isec" class="Q">Intersections, complements</a>
|
106
|
+
<p></p>
|
107
|
+
<p class="codebox">
|
108
|
+
# Include positions which appear in at least two files <br>
|
109
|
+
<a href="perl_module.html#vcf-isec">vcf-isec</a> -o -n +2 A.vcf.gz B.vcf.gz C.vcf.gz | bgzip -c > out.vcf.gz <br>
|
110
|
+
<br>
|
111
|
+
# Exclude from A positions which appear in B and/or C <br>
|
112
|
+
<a href="perl_module.html#vcf-isec">vcf-isec</a> -c A.vcf.gz B.vcf.gz C.vcf.gz | bgzip -c > out.vcf.gz <br>
|
113
|
+
</p>
|
114
|
+
</div>
|
115
|
+
|
116
|
+
|
117
|
+
<div class="item">
|
118
|
+
<a name="merge" class="Q">Merging</a>
|
119
|
+
<p></p>
|
120
|
+
<p class="codebox">
|
121
|
+
<a href="perl_module.html#vcf-merge">vcf-merge</a> A.vcf.gz B.vcf.gz | bgzip -c > C.vcf.gz
|
122
|
+
</p>
|
123
|
+
</div>
|
124
|
+
|
125
|
+
<div class="item">
|
126
|
+
<a name="isec" class="Q">Querying</a>
|
127
|
+
<p></p>
|
128
|
+
<p class="codebox"><a href="perl_module.html#vcf-query">vcf-query</a> file.vcf.gz 1:10327-10330 -c NA0001
|
129
|
+
</p>
|
130
|
+
</div>
|
131
|
+
|
132
|
+
|
133
|
+
|
134
|
+
<div class="item">
|
135
|
+
<a name="shuffle" class="Q">Reordering columns</a>
|
136
|
+
<p></p>
|
137
|
+
<p class="codebox"><a href="perl_module.html#vcf-shuffle-cols">vcf-shuffle-cols</a> -t template.vcf.gz file.vcf.gz > out.vcf
|
138
|
+
</p>
|
139
|
+
</div>
|
140
|
+
|
141
|
+
|
142
|
+
<div class="item">
|
143
|
+
<a name="stats" class="Q">Stats</a>
|
144
|
+
<p></p>
|
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|
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<p class="codebox"><a href="perl_module.html#vcf-stats">vcf-stats</a> file.vcf.gz
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</p>
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</div>
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|
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|
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<div class="item">
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<a name="subset" class="Q">Stripping columns</a>
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<p></p>
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<p class="codebox"><a href="perl_module.html#vcf-subset">vcf-subset</a> -c NA0001,NA0002 file.vcf.gz | bgzip -c > out.vcf.gz
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</p>
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</div>
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<div class="item">
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<a name="one-liners" class="Q">Useful shell one-liners</a>
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<p> This sections lists some usefull one line commands. Note that there are also dedicated convenience scripts
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<a href="perl_module.html#vcf-sort">vcf-sort</a> and <a href="perl_module.html#vcf-concat">vcf-concat</a>
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which do the same but also perform some basic sanity checks.
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All examples in BASH.</p>
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<p class="codebox">
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# Replace VCF header. The file must be compressed by bgzip. <br>
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tabix -r header.txt in.vcf.gz > out.vcf.gz <br>
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<br>
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# Sort VCF file keeping the header. The head command is for performance. <br>
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(zcat file.vcf.gz | head -100 | grep ^#; <br>
|
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zcat file.vcf.gz | grep -v ^# | sort -k1,1d -k2,2n;) \ <br>
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| bgzip -c > out.vcf.gz <br>
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<br>
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# Merge (that is, concatenate) two VCF files into one, keeping the header <br>
|
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# from first one only. <br>
|
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+
(zcat A.vcf.gz | head -100 | grep ^#; \ <br>
|
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zcat A.vcf.gz | grep -v ^#; \ <br>
|
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zcat B.vcf.gz | grep -v ^#; ) \ <br>
|
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| bgzip -c > out.vcf.gz
|
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</p>
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</div>
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|
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<div class="item">
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<a name="validator" class="Q">VCF validation</a>
|
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<p> Both <span class="cmd">vcftools</span> and <span class="cmd">Vcf.pm</span> can be used for validation. The
|
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first validates VCFv4.0, the latter is able to validate the older versions as well.
|
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</p>
|
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<p class="codebox">
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perl -M<a href="perl_module.html#Vcf.pm">Vcf</a> -e validate example.vcf <br>
|
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perl -I/path/to/the/module/ -M<a href="perl_module.html#Vcf.pm">Vcf</a> -e validate example.vcf <br>
|
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<a href="perl_module.html#vcf-validator">vcf-validator</a> example.vcf
|
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</p>
|
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</div>
|
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|
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|
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<div class="item">
|
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<a name="more" class="Q">...and more</a>
|
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|
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<p>This page gives just a list of basic capabilities. For more, please go to the <a href="options.html">vcftools's options</a> page
|
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and the <a href="perl_module.html">Perl API and scripts</a> page.</p>
|
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</div>
|
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|
@@ -0,0 +1,52 @@
|
|
1
|
+
|
2
|
+
<div class="item">
|
3
|
+
<h1>Welcome to VCFtools</h1>
|
4
|
+
<p>Welcome to VCFtools - a program package designed for working with VCF
|
5
|
+
files, such as those generated by the 1000 Genomes Project. The aim of
|
6
|
+
VCFtools is to provide methods for working with VCF files: validating,
|
7
|
+
merging, comparing and calculate some basic population genetic statistics.
|
8
|
+
</p>
|
9
|
+
</div>
|
10
|
+
|
11
|
+
<div class="item">
|
12
|
+
<h3>Supported VCF versions</h3>
|
13
|
+
<p>VCFtools supports the <a href="specs.html">VCF format v4.0</a>. The
|
14
|
+
vcf-validator, Perl API and scripts now support also <a href="specs.html">VCF format v4.1</a> and
|
15
|
+
maintain backward compatibility with older versions.
|
16
|
+
<br>
|
17
|
+
For details, please go to the <a href="docs.html">Documentation</a> page.</p>
|
18
|
+
</div>
|
19
|
+
|
20
|
+
<div class="item">
|
21
|
+
<h3>Mailing List</h3>
|
22
|
+
<p>Anything VCF or VCFtools related may be discussed on the project's <a href="http://sourceforge.net/mail/?group_id=279407">mailing list</a>.</p>
|
23
|
+
</div>
|
24
|
+
|
25
|
+
<div class="item">
|
26
|
+
<h3>Download</h3>
|
27
|
+
<p>
|
28
|
+
The <b>latest stable release</b> can be downloaded from here:</p>
|
29
|
+
<p class="codebox" style="text-align: center;"><a href="https://sourceforge.net/projects/vcftools/files/">https://sourceforge.net/projects/vcftools/files/</a></p>
|
30
|
+
<p>
|
31
|
+
The <b>latest development version</b> can be retrieved by running the following command:
|
32
|
+
</p>
|
33
|
+
<p class="codebox" style="text-align: center;"> svn checkout https://vcftools.svn.sourceforge.net/svnroot/vcftools vcftools </p>
|
34
|
+
<p>
|
35
|
+
The above command is required to be run only once, for any subsequent updates run this command from the vcftools directory:
|
36
|
+
</p>
|
37
|
+
<p class="codebox" style="text-align: center;"> svn update </p>
|
38
|
+
</div>
|
39
|
+
<div class="item">
|
40
|
+
<h3>How to use</h3>
|
41
|
+
The VCFtools package includes a set of tools for
|
42
|
+
<ul>
|
43
|
+
<li> validating
|
44
|
+
<li> comparing
|
45
|
+
<li> merging
|
46
|
+
<li> annotating
|
47
|
+
<li> creating intersections and subsets
|
48
|
+
<li> ...
|
49
|
+
</ul>
|
50
|
+
For details, please go to the <a href="docs.html">Documentation</a> page.
|
51
|
+
</div>
|
52
|
+
|
@@ -0,0 +1,80 @@
|
|
1
|
+
<?php
|
2
|
+
|
3
|
+
function validate_path($path)
|
4
|
+
{
|
5
|
+
if ( preg_match("/^[A-Za-z0-9_]+$/",$path) && file_exists("src/$path.inc") ) return $path;
|
6
|
+
return "index";
|
7
|
+
}
|
8
|
+
|
9
|
+
$titles = array(
|
10
|
+
'index' => 'VCFtools',
|
11
|
+
'perl_module' => 'VCFtools: Perl tools and API',
|
12
|
+
'docs' => 'VCFtools Documentation',
|
13
|
+
'license' => 'VCFtools License',
|
14
|
+
'specs' => 'VCF Specification',
|
15
|
+
'links' => 'VCF Links',
|
16
|
+
'options' => 'vcftools Options',
|
17
|
+
);
|
18
|
+
|
19
|
+
if (isset($argc)) { $_GET['pg']=$argv[1]; }
|
20
|
+
$path = array_key_exists('pg',$_GET) ? validate_path($_GET['pg']) : 'index';
|
21
|
+
$title = array_key_exists($path,$titles) ? $titles[$path] : $titles['index'];
|
22
|
+
|
23
|
+
|
24
|
+
?>
|
25
|
+
<!DOCTYPE html PUBLIC "-//W3C//DTD HTML 4.01//EN" "http://www.w3.org/TR/html4/strict.dtd">
|
26
|
+
<html>
|
27
|
+
<head>
|
28
|
+
<script type="text/javascript">
|
29
|
+
var gaJsHost = (("https:" == document.location.protocol) ? "https://ssl." : "http://www.");
|
30
|
+
document.write(unescape("%3Cscript src='" + gaJsHost + "google-analytics.com/ga.js' type='text/javascript'%3E%3C/script%3E"));
|
31
|
+
</script>
|
32
|
+
<script type="text/javascript">
|
33
|
+
try {
|
34
|
+
var pageTracker = _gat._getTracker("UA-272183-4");
|
35
|
+
pageTracker._trackPageview();
|
36
|
+
} catch(err) {}
|
37
|
+
</script>
|
38
|
+
|
39
|
+
<meta http-equiv="content-type" content="text/html; charset=ISO-8859-1">
|
40
|
+
<link rel="stylesheet" type="text/css" href="default.css" media="screen">
|
41
|
+
<link href='favicon.png' rel='shortcut icon' type='image/png'>
|
42
|
+
<link href='favicon.png' rel='icon' type='image/png'>
|
43
|
+
|
44
|
+
<title><?php echo $title; ?></title>
|
45
|
+
</head>
|
46
|
+
|
47
|
+
|
48
|
+
|
49
|
+
<body>
|
50
|
+
<div class="container">
|
51
|
+
<div class="main">
|
52
|
+
<div class="header">
|
53
|
+
<div class="title">
|
54
|
+
<a href="index.html">VCFtools</a>
|
55
|
+
</div>
|
56
|
+
</div>
|
57
|
+
<div class="content">
|
58
|
+
|
59
|
+
<?php
|
60
|
+
include("$path.inc");
|
61
|
+
?>
|
62
|
+
|
63
|
+
</div>
|
64
|
+
|
65
|
+
<div class="sidenav">
|
66
|
+
<h1>Navigation</h1>
|
67
|
+
<ul>
|
68
|
+
<li><a href="index.html">Main</a></li>
|
69
|
+
<li><a href="http://sourceforge.net/projects/vcftools/">Sourceforge page</a></li>
|
70
|
+
<li><a href="docs.html">Documentation</a></li>
|
71
|
+
<li><a href="license.html">License</a></li>
|
72
|
+
<li><a href="specs.html">VCF specification</a></li>
|
73
|
+
<li><a href="links.html">Links</a></li>
|
74
|
+
<li><a href="http://www.1000genomes.org/">1000 Genomes</a></li>
|
75
|
+
</ul>
|
76
|
+
</div>
|
77
|
+
<div class="clearer"><span></span></div>
|
78
|
+
</div>
|
79
|
+
</div>
|
80
|
+
</body></html>
|