ngs_server 0.1 → 0.2
This diff represents the content of publicly available package versions that have been released to one of the supported registries. The information contained in this diff is provided for informational purposes only and reflects changes between package versions as they appear in their respective public registries.
- data/bin/ngs_server +72 -50
- data/ext/bamtools/extconf.rb +3 -3
- data/ext/vcftools/Makefile +28 -0
- data/ext/vcftools/README.txt +36 -0
- data/ext/vcftools/cpp/.svn/all-wcprops +125 -0
- data/ext/vcftools/cpp/.svn/dir-prop-base +6 -0
- data/ext/vcftools/cpp/.svn/entries +708 -0
- data/ext/vcftools/cpp/.svn/text-base/Makefile.svn-base +46 -0
- data/ext/vcftools/cpp/.svn/text-base/dgeev.cpp.svn-base +146 -0
- data/ext/vcftools/cpp/.svn/text-base/dgeev.h.svn-base +43 -0
- data/ext/vcftools/cpp/.svn/text-base/output_log.cpp.svn-base +79 -0
- data/ext/vcftools/cpp/.svn/text-base/output_log.h.svn-base +34 -0
- data/ext/vcftools/cpp/.svn/text-base/parameters.cpp.svn-base +535 -0
- data/ext/vcftools/cpp/.svn/text-base/parameters.h.svn-base +154 -0
- data/ext/vcftools/cpp/.svn/text-base/vcf_entry.cpp.svn-base +497 -0
- data/ext/vcftools/cpp/.svn/text-base/vcf_entry.h.svn-base +190 -0
- data/ext/vcftools/cpp/.svn/text-base/vcf_entry_getters.cpp.svn-base +421 -0
- data/ext/vcftools/cpp/.svn/text-base/vcf_entry_setters.cpp.svn-base +482 -0
- data/ext/vcftools/cpp/.svn/text-base/vcf_file.cpp.svn-base +495 -0
- data/ext/vcftools/cpp/.svn/text-base/vcf_file.h.svn-base +184 -0
- data/ext/vcftools/cpp/.svn/text-base/vcf_file_diff.cpp.svn-base +1282 -0
- data/ext/vcftools/cpp/.svn/text-base/vcf_file_filters.cpp.svn-base +1215 -0
- data/ext/vcftools/cpp/.svn/text-base/vcf_file_format_convert.cpp.svn-base +1138 -0
- data/ext/vcftools/cpp/.svn/text-base/vcf_file_index.cpp.svn-base +171 -0
- data/ext/vcftools/cpp/.svn/text-base/vcf_file_output.cpp.svn-base +3012 -0
- data/ext/vcftools/cpp/.svn/text-base/vcftools.cpp.svn-base +107 -0
- data/ext/vcftools/cpp/.svn/text-base/vcftools.h.svn-base +25 -0
- data/ext/vcftools/cpp/Makefile +46 -0
- data/ext/vcftools/cpp/dgeev.cpp +146 -0
- data/ext/vcftools/cpp/dgeev.h +43 -0
- data/ext/vcftools/cpp/output_log.cpp +79 -0
- data/ext/vcftools/cpp/output_log.h +34 -0
- data/ext/vcftools/cpp/parameters.cpp +535 -0
- data/ext/vcftools/cpp/parameters.h +154 -0
- data/ext/vcftools/cpp/vcf_entry.cpp +497 -0
- data/ext/vcftools/cpp/vcf_entry.h +190 -0
- data/ext/vcftools/cpp/vcf_entry_getters.cpp +421 -0
- data/ext/vcftools/cpp/vcf_entry_setters.cpp +482 -0
- data/ext/vcftools/cpp/vcf_file.cpp +495 -0
- data/ext/vcftools/cpp/vcf_file.h +184 -0
- data/ext/vcftools/cpp/vcf_file_diff.cpp +1282 -0
- data/ext/vcftools/cpp/vcf_file_filters.cpp +1215 -0
- data/ext/vcftools/cpp/vcf_file_format_convert.cpp +1138 -0
- data/ext/vcftools/cpp/vcf_file_index.cpp +171 -0
- data/ext/vcftools/cpp/vcf_file_output.cpp +3012 -0
- data/ext/vcftools/cpp/vcftools.cpp +107 -0
- data/ext/vcftools/cpp/vcftools.h +25 -0
- data/ext/vcftools/examples/.svn/all-wcprops +185 -0
- data/ext/vcftools/examples/.svn/dir-prop-base +6 -0
- data/ext/vcftools/examples/.svn/entries +1048 -0
- data/ext/vcftools/examples/.svn/prop-base/perl-api-1.pl.svn-base +5 -0
- data/ext/vcftools/examples/.svn/text-base/annotate-test.vcf.svn-base +37 -0
- data/ext/vcftools/examples/.svn/text-base/annotate.out.svn-base +23 -0
- data/ext/vcftools/examples/.svn/text-base/annotate.txt.svn-base +7 -0
- data/ext/vcftools/examples/.svn/text-base/annotate2.out.svn-base +52 -0
- data/ext/vcftools/examples/.svn/text-base/annotate3.out.svn-base +23 -0
- data/ext/vcftools/examples/.svn/text-base/cmp-test-a-3.3.vcf.svn-base +12 -0
- data/ext/vcftools/examples/.svn/text-base/cmp-test-a.vcf.svn-base +12 -0
- data/ext/vcftools/examples/.svn/text-base/cmp-test-b-3.3.vcf.svn-base +12 -0
- data/ext/vcftools/examples/.svn/text-base/cmp-test-b.vcf.svn-base +12 -0
- data/ext/vcftools/examples/.svn/text-base/cmp-test.out.svn-base +53 -0
- data/ext/vcftools/examples/.svn/text-base/concat-a.vcf.svn-base +21 -0
- data/ext/vcftools/examples/.svn/text-base/concat-b.vcf.svn-base +13 -0
- data/ext/vcftools/examples/.svn/text-base/concat-c.vcf.svn-base +19 -0
- data/ext/vcftools/examples/.svn/text-base/concat.out.svn-base +39 -0
- data/ext/vcftools/examples/.svn/text-base/invalid-4.0.vcf.svn-base +31 -0
- data/ext/vcftools/examples/.svn/text-base/isec-n2-test.vcf.out.svn-base +19 -0
- data/ext/vcftools/examples/.svn/text-base/merge-test-a.vcf.svn-base +17 -0
- data/ext/vcftools/examples/.svn/text-base/merge-test-b.vcf.svn-base +17 -0
- data/ext/vcftools/examples/.svn/text-base/merge-test-c.vcf.svn-base +15 -0
- data/ext/vcftools/examples/.svn/text-base/merge-test.vcf.out.svn-base +31 -0
- data/ext/vcftools/examples/.svn/text-base/perl-api-1.pl.svn-base +46 -0
- data/ext/vcftools/examples/.svn/text-base/query-test.out.svn-base +6 -0
- data/ext/vcftools/examples/.svn/text-base/shuffle-test.vcf.svn-base +12 -0
- data/ext/vcftools/examples/.svn/text-base/subset.SNPs.out.svn-base +10 -0
- data/ext/vcftools/examples/.svn/text-base/subset.indels.out.svn-base +18 -0
- data/ext/vcftools/examples/.svn/text-base/subset.vcf.svn-base +21 -0
- data/ext/vcftools/examples/.svn/text-base/valid-3.3.vcf.svn-base +30 -0
- data/ext/vcftools/examples/.svn/text-base/valid-4.0.vcf.stats.svn-base +104 -0
- data/ext/vcftools/examples/.svn/text-base/valid-4.0.vcf.svn-base +34 -0
- data/ext/vcftools/examples/.svn/text-base/valid-4.1.vcf.svn-base +37 -0
- data/ext/vcftools/examples/annotate-test.vcf +37 -0
- data/ext/vcftools/examples/annotate.out +23 -0
- data/ext/vcftools/examples/annotate.txt +7 -0
- data/ext/vcftools/examples/annotate2.out +52 -0
- data/ext/vcftools/examples/annotate3.out +23 -0
- data/ext/vcftools/examples/cmp-test-a-3.3.vcf +12 -0
- data/ext/vcftools/examples/cmp-test-a.vcf +12 -0
- data/ext/vcftools/examples/cmp-test-b-3.3.vcf +12 -0
- data/ext/vcftools/examples/cmp-test-b.vcf +12 -0
- data/ext/vcftools/examples/cmp-test.out +53 -0
- data/ext/vcftools/examples/concat-a.vcf +21 -0
- data/ext/vcftools/examples/concat-b.vcf +13 -0
- data/ext/vcftools/examples/concat-c.vcf +19 -0
- data/ext/vcftools/examples/concat.out +39 -0
- data/ext/vcftools/examples/invalid-4.0.vcf +31 -0
- data/ext/vcftools/examples/isec-n2-test.vcf.out +19 -0
- data/ext/vcftools/examples/merge-test-a.vcf +17 -0
- data/ext/vcftools/examples/merge-test-b.vcf +17 -0
- data/ext/vcftools/examples/merge-test-c.vcf +15 -0
- data/ext/vcftools/examples/merge-test.vcf.out +31 -0
- data/ext/vcftools/examples/perl-api-1.pl +46 -0
- data/ext/vcftools/examples/query-test.out +6 -0
- data/ext/vcftools/examples/shuffle-test.vcf +12 -0
- data/ext/vcftools/examples/subset.SNPs.out +10 -0
- data/ext/vcftools/examples/subset.indels.out +18 -0
- data/ext/vcftools/examples/subset.vcf +21 -0
- data/ext/vcftools/examples/valid-3.3.vcf +30 -0
- data/ext/vcftools/examples/valid-4.0.vcf +34 -0
- data/ext/vcftools/examples/valid-4.0.vcf.stats +104 -0
- data/ext/vcftools/examples/valid-4.1.vcf +37 -0
- data/ext/vcftools/extconf.rb +2 -0
- data/ext/vcftools/perl/.svn/all-wcprops +149 -0
- data/ext/vcftools/perl/.svn/entries +844 -0
- data/ext/vcftools/perl/.svn/prop-base/fill-aa.svn-base +5 -0
- data/ext/vcftools/perl/.svn/prop-base/fill-an-ac.svn-base +5 -0
- data/ext/vcftools/perl/.svn/prop-base/fill-ref-md5.svn-base +5 -0
- data/ext/vcftools/perl/.svn/prop-base/tab-to-vcf.svn-base +5 -0
- data/ext/vcftools/perl/.svn/prop-base/test.t.svn-base +5 -0
- data/ext/vcftools/perl/.svn/prop-base/vcf-annotate.svn-base +5 -0
- data/ext/vcftools/perl/.svn/prop-base/vcf-compare.svn-base +5 -0
- data/ext/vcftools/perl/.svn/prop-base/vcf-concat.svn-base +5 -0
- data/ext/vcftools/perl/.svn/prop-base/vcf-convert.svn-base +5 -0
- data/ext/vcftools/perl/.svn/prop-base/vcf-fix-newlines.svn-base +5 -0
- data/ext/vcftools/perl/.svn/prop-base/vcf-isec.svn-base +5 -0
- data/ext/vcftools/perl/.svn/prop-base/vcf-merge.svn-base +5 -0
- data/ext/vcftools/perl/.svn/prop-base/vcf-query.svn-base +5 -0
- data/ext/vcftools/perl/.svn/prop-base/vcf-shuffle-cols.svn-base +5 -0
- data/ext/vcftools/perl/.svn/prop-base/vcf-sort.svn-base +5 -0
- data/ext/vcftools/perl/.svn/prop-base/vcf-stats.svn-base +5 -0
- data/ext/vcftools/perl/.svn/prop-base/vcf-subset.svn-base +5 -0
- data/ext/vcftools/perl/.svn/prop-base/vcf-to-tab.svn-base +5 -0
- data/ext/vcftools/perl/.svn/prop-base/vcf-validator.svn-base +5 -0
- data/ext/vcftools/perl/.svn/text-base/ChangeLog.svn-base +84 -0
- data/ext/vcftools/perl/.svn/text-base/FaSlice.pm.svn-base +214 -0
- data/ext/vcftools/perl/.svn/text-base/Makefile.svn-base +12 -0
- data/ext/vcftools/perl/.svn/text-base/Vcf.pm.svn-base +2853 -0
- data/ext/vcftools/perl/.svn/text-base/VcfStats.pm.svn-base +681 -0
- data/ext/vcftools/perl/.svn/text-base/fill-aa.svn-base +103 -0
- data/ext/vcftools/perl/.svn/text-base/fill-an-ac.svn-base +56 -0
- data/ext/vcftools/perl/.svn/text-base/fill-ref-md5.svn-base +204 -0
- data/ext/vcftools/perl/.svn/text-base/tab-to-vcf.svn-base +92 -0
- data/ext/vcftools/perl/.svn/text-base/test.t.svn-base +376 -0
- data/ext/vcftools/perl/.svn/text-base/vcf-annotate.svn-base +1099 -0
- data/ext/vcftools/perl/.svn/text-base/vcf-compare.svn-base +1193 -0
- data/ext/vcftools/perl/.svn/text-base/vcf-concat.svn-base +310 -0
- data/ext/vcftools/perl/.svn/text-base/vcf-convert.svn-base +180 -0
- data/ext/vcftools/perl/.svn/text-base/vcf-fix-newlines.svn-base +97 -0
- data/ext/vcftools/perl/.svn/text-base/vcf-isec.svn-base +660 -0
- data/ext/vcftools/perl/.svn/text-base/vcf-merge.svn-base +577 -0
- data/ext/vcftools/perl/.svn/text-base/vcf-query.svn-base +272 -0
- data/ext/vcftools/perl/.svn/text-base/vcf-shuffle-cols.svn-base +89 -0
- data/ext/vcftools/perl/.svn/text-base/vcf-sort.svn-base +79 -0
- data/ext/vcftools/perl/.svn/text-base/vcf-stats.svn-base +160 -0
- data/ext/vcftools/perl/.svn/text-base/vcf-subset.svn-base +206 -0
- data/ext/vcftools/perl/.svn/text-base/vcf-to-tab.svn-base +112 -0
- data/ext/vcftools/perl/.svn/text-base/vcf-validator.svn-base +145 -0
- data/ext/vcftools/perl/ChangeLog +84 -0
- data/ext/vcftools/perl/FaSlice.pm +214 -0
- data/ext/vcftools/perl/Makefile +12 -0
- data/ext/vcftools/perl/Vcf.pm +2853 -0
- data/ext/vcftools/perl/VcfStats.pm +681 -0
- data/ext/vcftools/perl/fill-aa +103 -0
- data/ext/vcftools/perl/fill-an-ac +56 -0
- data/ext/vcftools/perl/fill-ref-md5 +204 -0
- data/ext/vcftools/perl/tab-to-vcf +92 -0
- data/ext/vcftools/perl/test.t +376 -0
- data/ext/vcftools/perl/vcf-annotate +1099 -0
- data/ext/vcftools/perl/vcf-compare +1193 -0
- data/ext/vcftools/perl/vcf-concat +310 -0
- data/ext/vcftools/perl/vcf-convert +180 -0
- data/ext/vcftools/perl/vcf-fix-newlines +97 -0
- data/ext/vcftools/perl/vcf-isec +660 -0
- data/ext/vcftools/perl/vcf-merge +577 -0
- data/ext/vcftools/perl/vcf-query +286 -0
- data/ext/vcftools/perl/vcf-shuffle-cols +89 -0
- data/ext/vcftools/perl/vcf-sort +79 -0
- data/ext/vcftools/perl/vcf-stats +160 -0
- data/ext/vcftools/perl/vcf-subset +206 -0
- data/ext/vcftools/perl/vcf-to-tab +112 -0
- data/ext/vcftools/perl/vcf-validator +145 -0
- data/ext/vcftools/website/.svn/all-wcprops +41 -0
- data/ext/vcftools/website/.svn/entries +238 -0
- data/ext/vcftools/website/.svn/prop-base/VCF-poster.pdf.svn-base +5 -0
- data/ext/vcftools/website/.svn/prop-base/favicon.ico.svn-base +5 -0
- data/ext/vcftools/website/.svn/prop-base/favicon.png.svn-base +5 -0
- data/ext/vcftools/website/.svn/text-base/Makefile.svn-base +6 -0
- data/ext/vcftools/website/.svn/text-base/README.svn-base +2 -0
- data/ext/vcftools/website/.svn/text-base/VCF-poster.pdf.svn-base +0 -0
- data/ext/vcftools/website/.svn/text-base/default.css.svn-base +250 -0
- data/ext/vcftools/website/.svn/text-base/favicon.ico.svn-base +0 -0
- data/ext/vcftools/website/.svn/text-base/favicon.png.svn-base +0 -0
- data/ext/vcftools/website/Makefile +6 -0
- data/ext/vcftools/website/README +2 -0
- data/ext/vcftools/website/VCF-poster.pdf +0 -0
- data/ext/vcftools/website/default.css +250 -0
- data/ext/vcftools/website/favicon.ico +0 -0
- data/ext/vcftools/website/favicon.png +0 -0
- data/ext/vcftools/website/img/.svn/all-wcprops +53 -0
- data/ext/vcftools/website/img/.svn/entries +300 -0
- data/ext/vcftools/website/img/.svn/prop-base/bg.gif.svn-base +5 -0
- data/ext/vcftools/website/img/.svn/prop-base/bgcode.gif.svn-base +5 -0
- data/ext/vcftools/website/img/.svn/prop-base/bgcontainer.gif.svn-base +5 -0
- data/ext/vcftools/website/img/.svn/prop-base/bgul.gif.svn-base +5 -0
- data/ext/vcftools/website/img/.svn/prop-base/header.gif.svn-base +5 -0
- data/ext/vcftools/website/img/.svn/prop-base/li.gif.svn-base +5 -0
- data/ext/vcftools/website/img/.svn/prop-base/quote.gif.svn-base +5 -0
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- data/ext/vcftools/website/img/.svn/text-base/search.gif.svn-base +0 -0
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- data/ext/vcftools/website/img/bgcode.gif +0 -0
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- data/ext/vcftools/website/src/.svn/all-wcprops +53 -0
- data/ext/vcftools/website/src/.svn/entries +300 -0
- data/ext/vcftools/website/src/.svn/text-base/docs.inc.svn-base +202 -0
- data/ext/vcftools/website/src/.svn/text-base/index.inc.svn-base +52 -0
- data/ext/vcftools/website/src/.svn/text-base/index.php.svn-base +80 -0
- data/ext/vcftools/website/src/.svn/text-base/license.inc.svn-base +27 -0
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- data/ext/vcftools/website/src/.svn/text-base/perl_module.inc.svn-base +249 -0
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- data/ext/vcftools/website/src/perl_module.inc +249 -0
- data/ext/vcftools/website/src/specs.inc +18 -0
- data/lib/config.ru +9 -0
- data/lib/ngs_server/add.rb +9 -0
- data/lib/ngs_server/version.rb +1 -1
- data/lib/ngs_server.rb +55 -3
- data/ngs_server.gemspec +5 -2
- metadata +296 -6
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<div class="item">
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<h3>Documentation</h3>
|
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<p>
|
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The VCFtools package is broadly split into two sections: <br>
|
|
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<ul class="faq">
|
|
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|
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<li> The <span class="cmd">vcftools</span> binary program, generally used to analyse VCF files.
|
|
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|
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<li> The <span class="cmd">Vcf.pm</span> perl module, which is a general Perl API containing a core of
|
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the utilities <span class="cmd">vcf-convert</span>, <span class="cmd">vcf-merge</span>,
|
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<span class="cmd">vcf-compare</span>, <span class="cmd">vcf-isec</span>, and others.
|
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|
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</p>
|
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<h3>Documentation</h3>
|
|
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|
|
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|
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<li> <a href="options.html">Documentation for the binary executable <span class="cmd">vcftools</span></a>
|
|
18
|
+
<li> <a href="perl_module.html">The Perl API and scripts</a>
|
|
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|
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<li> <a href="#one-liners">Useful shell one-liners</a>
|
|
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|
+
</ul>
|
|
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|
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|
|
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|
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<h3>Examples of usage by topic</h3>
|
|
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|
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<ul class="faq">
|
|
24
|
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<li> <a href="#install">Installation</a>
|
|
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|
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<li> <a href="#annotate">Annotating</a>
|
|
26
|
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<li> <a href="#compare">Comparing</a>
|
|
27
|
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<li> <a href="#concat">Concatenating</a>
|
|
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|
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<li> <a href="#convert">Converting</a>
|
|
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|
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<li> <a href="#isec">Intersections, complements</a>
|
|
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|
+
<li> <a href="#merge">Merging</a>
|
|
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|
+
<li> <a href="#query">Querying</a>
|
|
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|
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<li> <a href="#shuffle">Reordering columns</a>
|
|
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|
+
<li> <a href="#stats">Stats</a>
|
|
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|
+
<li> <a href="#subset">Stripping columns</a>
|
|
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|
+
<li> <a href="#one-liners">Useful shell one-liners</a>
|
|
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|
+
<li> <a href="#validator">Validating</a>
|
|
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|
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<li> <a href="#more">...and more</a>
|
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|
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</ul>
|
|
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|
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</div>
|
|
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|
|
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|
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|
|
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|
+
<div class="item">
|
|
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|
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<a name="install" class="Q">Installation</a>
|
|
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|
+
<p> The VCFtools package can be decompressed by the command
|
|
45
|
+
<p class="codebox">tar -xzf vcftools_<i>version_number</i>_source.tar.gz</p>
|
|
46
|
+
<p> To build the <span class="cmd">vcftools</span> executable, type "make" in the vcftools folder. </p>
|
|
47
|
+
<p>
|
|
48
|
+
The <b>Perl scripts require that VCF files are compressed by <span class="cmd">bgzip</span>
|
|
49
|
+
and indexed by <span class="cmd">tabix</span></b> (both tools are part of the tabix package,
|
|
50
|
+
available for <a href="https://sourceforge.net/projects/samtools/files/tabix/">download here</a>).
|
|
51
|
+
Test the tools can be tested by running the script
|
|
52
|
+
<p class="codebox">/path/to/your/installation/perl/test.t</p>
|
|
53
|
+
For running the Perl scripts, the <b>PERL5LIB environment variable must be set</b> to include the Vcf.pm
|
|
54
|
+
module</p>
|
|
55
|
+
<p class="codebox">export PERL5LIB=/path/to/your/installation/perl</p>
|
|
56
|
+
</div>
|
|
57
|
+
|
|
58
|
+
|
|
59
|
+
<div class="item">
|
|
60
|
+
<a name="annotate" class="Q">Annotating</a>
|
|
61
|
+
<p></p>
|
|
62
|
+
<p class="codebox">
|
|
63
|
+
# Add custom annotations <br>
|
|
64
|
+
cat in.vcf | vcf-annotate -a annotations.gz \ <br>
|
|
65
|
+
-d key=INFO,ID=ANN,Number=1,Type=Integer,Description='My custom annotation' \ <br>
|
|
66
|
+
-c CHROM,FROM,TO,INFO/ANN > out.vcf <br>
|
|
67
|
+
<br>
|
|
68
|
+
# Apply SnpCluster filter <br>
|
|
69
|
+
cat in.vcf | <a href="perl_module.html#vcf-annotate">vcf-annotate</a> --filter SnpCluster=3,10 > out.vcf <br>
|
|
70
|
+
</p>
|
|
71
|
+
</div>
|
|
72
|
+
|
|
73
|
+
|
|
74
|
+
|
|
75
|
+
<div class="item">
|
|
76
|
+
<a name="compare" class="Q">Comparing</a>
|
|
77
|
+
<p></p>
|
|
78
|
+
<p class="codebox"><a href="perl_module.html#vcf-compare">vcf-compare</a> A.vcf.gz B.vcf.gz C.vcf.gz
|
|
79
|
+
</p>
|
|
80
|
+
</div>
|
|
81
|
+
|
|
82
|
+
|
|
83
|
+
<div class="item">
|
|
84
|
+
<a name="concat" class="Q">Concatenating</a>
|
|
85
|
+
<p></p>
|
|
86
|
+
<p class="codebox"><a href="perl_module.html#vcf-concat">vcf-concat</a> A.vcf.gz B.vcf.gz C.vcf.gz | bgzip -c > out.vcf.gz
|
|
87
|
+
</p>
|
|
88
|
+
</div>
|
|
89
|
+
|
|
90
|
+
|
|
91
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<div class="item">
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<a name="convert" class="Q">Converting</a>
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<p></p>
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<p class="codebox">
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# Convert between VCF versions <br>
|
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zcat file.vcf.gz | <a href="perl_module.html#vcf-convert">vcf-convert</a> -r reference.fa | bgzip -c > out.vcf.gz <br>
|
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<br>
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# Convert from VCF format to tab-delimited text file <br>
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zcat file.vcf.gz | <a href="perl_module.html#vcf-to-tab">vcf-to-tab</a> > out.tab <br>
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</p>
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</div>
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<div class="item">
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<a name="isec" class="Q">Intersections, complements</a>
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<p></p>
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<p class="codebox">
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# Include positions which appear in at least two files <br>
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<a href="perl_module.html#vcf-isec">vcf-isec</a> -o -n +2 A.vcf.gz B.vcf.gz C.vcf.gz | bgzip -c > out.vcf.gz <br>
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<br>
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# Exclude from A positions which appear in B and/or C <br>
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<a href="perl_module.html#vcf-isec">vcf-isec</a> -c A.vcf.gz B.vcf.gz C.vcf.gz | bgzip -c > out.vcf.gz <br>
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</p>
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</div>
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<div class="item">
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<a name="merge" class="Q">Merging</a>
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<p></p>
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<p class="codebox">
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<a href="perl_module.html#vcf-merge">vcf-merge</a> A.vcf.gz B.vcf.gz | bgzip -c > C.vcf.gz
|
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</p>
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</div>
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<div class="item">
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<a name="isec" class="Q">Querying</a>
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<p></p>
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<p class="codebox"><a href="perl_module.html#vcf-query">vcf-query</a> file.vcf.gz 1:10327-10330 -c NA0001
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</p>
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</div>
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<div class="item">
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<a name="shuffle" class="Q">Reordering columns</a>
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<p></p>
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<p class="codebox"><a href="perl_module.html#vcf-shuffle-cols">vcf-shuffle-cols</a> -t template.vcf.gz file.vcf.gz > out.vcf
|
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</p>
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</div>
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+
|
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141
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<div class="item">
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<a name="stats" class="Q">Stats</a>
|
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<p></p>
|
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<p class="codebox"><a href="perl_module.html#vcf-stats">vcf-stats</a> file.vcf.gz
|
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</p>
|
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</div>
|
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+
|
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149
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|
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150
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<div class="item">
|
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<a name="subset" class="Q">Stripping columns</a>
|
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+
<p></p>
|
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<p class="codebox"><a href="perl_module.html#vcf-subset">vcf-subset</a> -c NA0001,NA0002 file.vcf.gz | bgzip -c > out.vcf.gz
|
|
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|
+
</p>
|
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</div>
|
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156
|
+
|
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157
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+
|
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+
|
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159
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<div class="item">
|
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<a name="one-liners" class="Q">Useful shell one-liners</a>
|
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<p> This sections lists some usefull one line commands. Note that there are also dedicated convenience scripts
|
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+
<a href="perl_module.html#vcf-sort">vcf-sort</a> and <a href="perl_module.html#vcf-concat">vcf-concat</a>
|
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163
|
+
which do the same but also perform some basic sanity checks.
|
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|
+
All examples in BASH.</p>
|
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+
<p class="codebox">
|
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|
+
# Replace VCF header. The file must be compressed by bgzip. <br>
|
|
167
|
+
tabix -r header.txt in.vcf.gz > out.vcf.gz <br>
|
|
168
|
+
<br>
|
|
169
|
+
# Sort VCF file keeping the header. The head command is for performance. <br>
|
|
170
|
+
(zcat file.vcf.gz | head -100 | grep ^#; <br>
|
|
171
|
+
zcat file.vcf.gz | grep -v ^# | sort -k1,1d -k2,2n;) \ <br>
|
|
172
|
+
| bgzip -c > out.vcf.gz <br>
|
|
173
|
+
<br>
|
|
174
|
+
# Merge (that is, concatenate) two VCF files into one, keeping the header <br>
|
|
175
|
+
# from first one only. <br>
|
|
176
|
+
(zcat A.vcf.gz | head -100 | grep ^#; \ <br>
|
|
177
|
+
zcat A.vcf.gz | grep -v ^#; \ <br>
|
|
178
|
+
zcat B.vcf.gz | grep -v ^#; ) \ <br>
|
|
179
|
+
| bgzip -c > out.vcf.gz
|
|
180
|
+
</p>
|
|
181
|
+
</div>
|
|
182
|
+
|
|
183
|
+
|
|
184
|
+
<div class="item">
|
|
185
|
+
<a name="validator" class="Q">VCF validation</a>
|
|
186
|
+
<p> Both <span class="cmd">vcftools</span> and <span class="cmd">Vcf.pm</span> can be used for validation. The
|
|
187
|
+
first validates VCFv4.0, the latter is able to validate the older versions as well.
|
|
188
|
+
</p>
|
|
189
|
+
<p class="codebox">
|
|
190
|
+
perl -M<a href="perl_module.html#Vcf.pm">Vcf</a> -e validate example.vcf <br>
|
|
191
|
+
perl -I/path/to/the/module/ -M<a href="perl_module.html#Vcf.pm">Vcf</a> -e validate example.vcf <br>
|
|
192
|
+
<a href="perl_module.html#vcf-validator">vcf-validator</a> example.vcf
|
|
193
|
+
</p>
|
|
194
|
+
</div>
|
|
195
|
+
|
|
196
|
+
|
|
197
|
+
<div class="item">
|
|
198
|
+
<a name="more" class="Q">...and more</a>
|
|
199
|
+
<p>This page gives just a list of basic capabilities. For more, please go to the <a href="options.html">vcftools's options</a> page
|
|
200
|
+
and the <a href="perl_module.html">Perl API and scripts</a> page.</p>
|
|
201
|
+
</div>
|
|
202
|
+
|
|
@@ -0,0 +1,52 @@
|
|
|
1
|
+
|
|
2
|
+
<div class="item">
|
|
3
|
+
<h1>Welcome to VCFtools</h1>
|
|
4
|
+
<p>Welcome to VCFtools - a program package designed for working with VCF
|
|
5
|
+
files, such as those generated by the 1000 Genomes Project. The aim of
|
|
6
|
+
VCFtools is to provide methods for working with VCF files: validating,
|
|
7
|
+
merging, comparing and calculate some basic population genetic statistics.
|
|
8
|
+
</p>
|
|
9
|
+
</div>
|
|
10
|
+
|
|
11
|
+
<div class="item">
|
|
12
|
+
<h3>Supported VCF versions</h3>
|
|
13
|
+
<p>VCFtools supports the <a href="specs.html">VCF format v4.0</a>. The
|
|
14
|
+
vcf-validator, Perl API and scripts now support also <a href="specs.html">VCF format v4.1</a> and
|
|
15
|
+
maintain backward compatibility with older versions.
|
|
16
|
+
<br>
|
|
17
|
+
For details, please go to the <a href="docs.html">Documentation</a> page.</p>
|
|
18
|
+
</div>
|
|
19
|
+
|
|
20
|
+
<div class="item">
|
|
21
|
+
<h3>Mailing List</h3>
|
|
22
|
+
<p>Anything VCF or VCFtools related may be discussed on the project's <a href="http://sourceforge.net/mail/?group_id=279407">mailing list</a>.</p>
|
|
23
|
+
</div>
|
|
24
|
+
|
|
25
|
+
<div class="item">
|
|
26
|
+
<h3>Download</h3>
|
|
27
|
+
<p>
|
|
28
|
+
The <b>latest stable release</b> can be downloaded from here:</p>
|
|
29
|
+
<p class="codebox" style="text-align: center;"><a href="https://sourceforge.net/projects/vcftools/files/">https://sourceforge.net/projects/vcftools/files/</a></p>
|
|
30
|
+
<p>
|
|
31
|
+
The <b>latest development version</b> can be retrieved by running the following command:
|
|
32
|
+
</p>
|
|
33
|
+
<p class="codebox" style="text-align: center;"> svn checkout https://vcftools.svn.sourceforge.net/svnroot/vcftools vcftools </p>
|
|
34
|
+
<p>
|
|
35
|
+
The above command is required to be run only once, for any subsequent updates run this command from the vcftools directory:
|
|
36
|
+
</p>
|
|
37
|
+
<p class="codebox" style="text-align: center;"> svn update </p>
|
|
38
|
+
</div>
|
|
39
|
+
<div class="item">
|
|
40
|
+
<h3>How to use</h3>
|
|
41
|
+
The VCFtools package includes a set of tools for
|
|
42
|
+
<ul>
|
|
43
|
+
<li> validating
|
|
44
|
+
<li> comparing
|
|
45
|
+
<li> merging
|
|
46
|
+
<li> annotating
|
|
47
|
+
<li> creating intersections and subsets
|
|
48
|
+
<li> ...
|
|
49
|
+
</ul>
|
|
50
|
+
For details, please go to the <a href="docs.html">Documentation</a> page.
|
|
51
|
+
</div>
|
|
52
|
+
|
|
@@ -0,0 +1,80 @@
|
|
|
1
|
+
<?php
|
|
2
|
+
|
|
3
|
+
function validate_path($path)
|
|
4
|
+
{
|
|
5
|
+
if ( preg_match("/^[A-Za-z0-9_]+$/",$path) && file_exists("src/$path.inc") ) return $path;
|
|
6
|
+
return "index";
|
|
7
|
+
}
|
|
8
|
+
|
|
9
|
+
$titles = array(
|
|
10
|
+
'index' => 'VCFtools',
|
|
11
|
+
'perl_module' => 'VCFtools: Perl tools and API',
|
|
12
|
+
'docs' => 'VCFtools Documentation',
|
|
13
|
+
'license' => 'VCFtools License',
|
|
14
|
+
'specs' => 'VCF Specification',
|
|
15
|
+
'links' => 'VCF Links',
|
|
16
|
+
'options' => 'vcftools Options',
|
|
17
|
+
);
|
|
18
|
+
|
|
19
|
+
if (isset($argc)) { $_GET['pg']=$argv[1]; }
|
|
20
|
+
$path = array_key_exists('pg',$_GET) ? validate_path($_GET['pg']) : 'index';
|
|
21
|
+
$title = array_key_exists($path,$titles) ? $titles[$path] : $titles['index'];
|
|
22
|
+
|
|
23
|
+
|
|
24
|
+
?>
|
|
25
|
+
<!DOCTYPE html PUBLIC "-//W3C//DTD HTML 4.01//EN" "http://www.w3.org/TR/html4/strict.dtd">
|
|
26
|
+
<html>
|
|
27
|
+
<head>
|
|
28
|
+
<script type="text/javascript">
|
|
29
|
+
var gaJsHost = (("https:" == document.location.protocol) ? "https://ssl." : "http://www.");
|
|
30
|
+
document.write(unescape("%3Cscript src='" + gaJsHost + "google-analytics.com/ga.js' type='text/javascript'%3E%3C/script%3E"));
|
|
31
|
+
</script>
|
|
32
|
+
<script type="text/javascript">
|
|
33
|
+
try {
|
|
34
|
+
var pageTracker = _gat._getTracker("UA-272183-4");
|
|
35
|
+
pageTracker._trackPageview();
|
|
36
|
+
} catch(err) {}
|
|
37
|
+
</script>
|
|
38
|
+
|
|
39
|
+
<meta http-equiv="content-type" content="text/html; charset=ISO-8859-1">
|
|
40
|
+
<link rel="stylesheet" type="text/css" href="default.css" media="screen">
|
|
41
|
+
<link href='favicon.png' rel='shortcut icon' type='image/png'>
|
|
42
|
+
<link href='favicon.png' rel='icon' type='image/png'>
|
|
43
|
+
|
|
44
|
+
<title><?php echo $title; ?></title>
|
|
45
|
+
</head>
|
|
46
|
+
|
|
47
|
+
|
|
48
|
+
|
|
49
|
+
<body>
|
|
50
|
+
<div class="container">
|
|
51
|
+
<div class="main">
|
|
52
|
+
<div class="header">
|
|
53
|
+
<div class="title">
|
|
54
|
+
<a href="index.html">VCFtools</a>
|
|
55
|
+
</div>
|
|
56
|
+
</div>
|
|
57
|
+
<div class="content">
|
|
58
|
+
|
|
59
|
+
<?php
|
|
60
|
+
include("$path.inc");
|
|
61
|
+
?>
|
|
62
|
+
|
|
63
|
+
</div>
|
|
64
|
+
|
|
65
|
+
<div class="sidenav">
|
|
66
|
+
<h1>Navigation</h1>
|
|
67
|
+
<ul>
|
|
68
|
+
<li><a href="index.html">Main</a></li>
|
|
69
|
+
<li><a href="http://sourceforge.net/projects/vcftools/">Sourceforge page</a></li>
|
|
70
|
+
<li><a href="docs.html">Documentation</a></li>
|
|
71
|
+
<li><a href="license.html">License</a></li>
|
|
72
|
+
<li><a href="specs.html">VCF specification</a></li>
|
|
73
|
+
<li><a href="links.html">Links</a></li>
|
|
74
|
+
<li><a href="http://www.1000genomes.org/">1000 Genomes</a></li>
|
|
75
|
+
</ul>
|
|
76
|
+
</div>
|
|
77
|
+
<div class="clearer"><span></span></div>
|
|
78
|
+
</div>
|
|
79
|
+
</div>
|
|
80
|
+
</body></html>
|