miga-base 1.2.17.0 → 1.2.17.2
Sign up to get free protection for your applications and to get access to all the features.
- checksums.yaml +4 -4
- data/lib/miga/version.rb +2 -2
- data/utils/FastAAI/00.Libraries/01.SCG_HMMs/Archaea_SCG.hmm +41964 -0
- data/utils/FastAAI/00.Libraries/01.SCG_HMMs/Bacteria_SCG.hmm +32439 -0
- data/utils/FastAAI/00.Libraries/01.SCG_HMMs/Complete_SCG_DB.hmm +62056 -0
- data/utils/FastAAI/FastAAI +3659 -0
- data/utils/FastAAI/FastAAI-legacy/FastAAI +1336 -0
- data/utils/FastAAI/FastAAI-legacy/kAAI_v1.0_virus.py +1296 -0
- data/utils/FastAAI/README.md +84 -0
- data/utils/enveomics/Docs/recplot2.md +244 -0
- data/utils/enveomics/Examples/aai-matrix.bash +66 -0
- data/utils/enveomics/Examples/ani-matrix.bash +66 -0
- data/utils/enveomics/Examples/essential-phylogeny.bash +105 -0
- data/utils/enveomics/Examples/unus-genome-phylogeny.bash +100 -0
- data/utils/enveomics/LICENSE.txt +73 -0
- data/utils/enveomics/Makefile +52 -0
- data/utils/enveomics/Manifest/Tasks/aasubs.json +103 -0
- data/utils/enveomics/Manifest/Tasks/blasttab.json +790 -0
- data/utils/enveomics/Manifest/Tasks/distances.json +161 -0
- data/utils/enveomics/Manifest/Tasks/fasta.json +802 -0
- data/utils/enveomics/Manifest/Tasks/fastq.json +291 -0
- data/utils/enveomics/Manifest/Tasks/graphics.json +126 -0
- data/utils/enveomics/Manifest/Tasks/mapping.json +165 -0
- data/utils/enveomics/Manifest/Tasks/ogs.json +382 -0
- data/utils/enveomics/Manifest/Tasks/other.json +906 -0
- data/utils/enveomics/Manifest/Tasks/remote.json +356 -0
- data/utils/enveomics/Manifest/Tasks/sequence-identity.json +650 -0
- data/utils/enveomics/Manifest/Tasks/tables.json +308 -0
- data/utils/enveomics/Manifest/Tasks/trees.json +68 -0
- data/utils/enveomics/Manifest/Tasks/variants.json +111 -0
- data/utils/enveomics/Manifest/categories.json +165 -0
- data/utils/enveomics/Manifest/examples.json +162 -0
- data/utils/enveomics/Manifest/tasks.json +4 -0
- data/utils/enveomics/README.md +42 -0
- data/utils/enveomics/Scripts/AAsubs.log2ratio.rb +171 -0
- data/utils/enveomics/Scripts/Aln.cat.rb +221 -0
- data/utils/enveomics/Scripts/Aln.convert.pl +35 -0
- data/utils/enveomics/Scripts/AlphaDiversity.pl +152 -0
- data/utils/enveomics/Scripts/BedGraph.tad.rb +138 -0
- data/utils/enveomics/Scripts/BedGraph.window.rb +71 -0
- data/utils/enveomics/Scripts/BlastPairwise.AAsubs.pl +102 -0
- data/utils/enveomics/Scripts/BlastTab.addlen.rb +63 -0
- data/utils/enveomics/Scripts/BlastTab.advance.bash +48 -0
- data/utils/enveomics/Scripts/BlastTab.best_hit_sorted.pl +55 -0
- data/utils/enveomics/Scripts/BlastTab.catsbj.pl +104 -0
- data/utils/enveomics/Scripts/BlastTab.cogCat.rb +76 -0
- data/utils/enveomics/Scripts/BlastTab.filter.pl +47 -0
- data/utils/enveomics/Scripts/BlastTab.kegg_pep2path_rest.pl +194 -0
- data/utils/enveomics/Scripts/BlastTab.metaxaPrep.pl +104 -0
- data/utils/enveomics/Scripts/BlastTab.pairedHits.rb +157 -0
- data/utils/enveomics/Scripts/BlastTab.recplot2.R +48 -0
- data/utils/enveomics/Scripts/BlastTab.seqdepth.pl +86 -0
- data/utils/enveomics/Scripts/BlastTab.seqdepth_ZIP.pl +119 -0
- data/utils/enveomics/Scripts/BlastTab.seqdepth_nomedian.pl +86 -0
- data/utils/enveomics/Scripts/BlastTab.subsample.pl +47 -0
- data/utils/enveomics/Scripts/BlastTab.sumPerHit.pl +114 -0
- data/utils/enveomics/Scripts/BlastTab.taxid2taxrank.pl +90 -0
- data/utils/enveomics/Scripts/BlastTab.topHits_sorted.rb +123 -0
- data/utils/enveomics/Scripts/Chao1.pl +97 -0
- data/utils/enveomics/Scripts/CharTable.classify.rb +234 -0
- data/utils/enveomics/Scripts/EBIseq2tax.rb +83 -0
- data/utils/enveomics/Scripts/FastA.N50.pl +60 -0
- data/utils/enveomics/Scripts/FastA.extract.rb +152 -0
- data/utils/enveomics/Scripts/FastA.filter.pl +52 -0
- data/utils/enveomics/Scripts/FastA.filterLen.pl +28 -0
- data/utils/enveomics/Scripts/FastA.filterN.pl +60 -0
- data/utils/enveomics/Scripts/FastA.fragment.rb +100 -0
- data/utils/enveomics/Scripts/FastA.gc.pl +42 -0
- data/utils/enveomics/Scripts/FastA.interpose.pl +93 -0
- data/utils/enveomics/Scripts/FastA.length.pl +38 -0
- data/utils/enveomics/Scripts/FastA.mask.rb +89 -0
- data/utils/enveomics/Scripts/FastA.per_file.pl +36 -0
- data/utils/enveomics/Scripts/FastA.qlen.pl +57 -0
- data/utils/enveomics/Scripts/FastA.rename.pl +65 -0
- data/utils/enveomics/Scripts/FastA.revcom.pl +23 -0
- data/utils/enveomics/Scripts/FastA.sample.rb +98 -0
- data/utils/enveomics/Scripts/FastA.slider.pl +85 -0
- data/utils/enveomics/Scripts/FastA.split.pl +55 -0
- data/utils/enveomics/Scripts/FastA.split.rb +79 -0
- data/utils/enveomics/Scripts/FastA.subsample.pl +131 -0
- data/utils/enveomics/Scripts/FastA.tag.rb +65 -0
- data/utils/enveomics/Scripts/FastA.toFastQ.rb +69 -0
- data/utils/enveomics/Scripts/FastA.wrap.rb +48 -0
- data/utils/enveomics/Scripts/FastQ.filter.pl +54 -0
- data/utils/enveomics/Scripts/FastQ.interpose.pl +90 -0
- data/utils/enveomics/Scripts/FastQ.maskQual.rb +89 -0
- data/utils/enveomics/Scripts/FastQ.offset.pl +90 -0
- data/utils/enveomics/Scripts/FastQ.split.pl +53 -0
- data/utils/enveomics/Scripts/FastQ.tag.rb +70 -0
- data/utils/enveomics/Scripts/FastQ.test-error.rb +81 -0
- data/utils/enveomics/Scripts/FastQ.toFastA.awk +24 -0
- data/utils/enveomics/Scripts/GFF.catsbj.pl +127 -0
- data/utils/enveomics/Scripts/GenBank.add_fields.rb +84 -0
- data/utils/enveomics/Scripts/HMM.essential.rb +351 -0
- data/utils/enveomics/Scripts/HMM.haai.rb +168 -0
- data/utils/enveomics/Scripts/HMMsearch.extractIds.rb +83 -0
- data/utils/enveomics/Scripts/JPlace.distances.rb +88 -0
- data/utils/enveomics/Scripts/JPlace.to_iToL.rb +320 -0
- data/utils/enveomics/Scripts/M5nr.getSequences.rb +81 -0
- data/utils/enveomics/Scripts/MeTaxa.distribution.pl +198 -0
- data/utils/enveomics/Scripts/MyTaxa.fragsByTax.pl +35 -0
- data/utils/enveomics/Scripts/MyTaxa.seq-taxrank.rb +49 -0
- data/utils/enveomics/Scripts/NCBIacc2tax.rb +92 -0
- data/utils/enveomics/Scripts/Newick.autoprune.R +27 -0
- data/utils/enveomics/Scripts/RAxML-EPA.to_iToL.pl +228 -0
- data/utils/enveomics/Scripts/RecPlot2.compareIdentities.R +32 -0
- data/utils/enveomics/Scripts/RefSeq.download.bash +48 -0
- data/utils/enveomics/Scripts/SRA.download.bash +67 -0
- data/utils/enveomics/Scripts/TRIBS.plot-test.R +36 -0
- data/utils/enveomics/Scripts/TRIBS.test.R +39 -0
- data/utils/enveomics/Scripts/Table.barplot.R +31 -0
- data/utils/enveomics/Scripts/Table.df2dist.R +30 -0
- data/utils/enveomics/Scripts/Table.filter.pl +61 -0
- data/utils/enveomics/Scripts/Table.merge.pl +77 -0
- data/utils/enveomics/Scripts/Table.prefScore.R +60 -0
- data/utils/enveomics/Scripts/Table.replace.rb +69 -0
- data/utils/enveomics/Scripts/Table.round.rb +63 -0
- data/utils/enveomics/Scripts/Table.split.pl +57 -0
- data/utils/enveomics/Scripts/Taxonomy.silva2ncbi.rb +227 -0
- data/utils/enveomics/Scripts/VCF.KaKs.rb +147 -0
- data/utils/enveomics/Scripts/VCF.SNPs.rb +88 -0
- data/utils/enveomics/Scripts/aai.rb +421 -0
- data/utils/enveomics/Scripts/ani.rb +362 -0
- data/utils/enveomics/Scripts/anir.rb +137 -0
- data/utils/enveomics/Scripts/clust.rand.rb +102 -0
- data/utils/enveomics/Scripts/gi2tax.rb +103 -0
- data/utils/enveomics/Scripts/in_silico_GA_GI.pl +96 -0
- data/utils/enveomics/Scripts/lib/data/dupont_2012_essential.hmm.gz +0 -0
- data/utils/enveomics/Scripts/lib/data/lee_2019_essential.hmm.gz +0 -0
- data/utils/enveomics/Scripts/lib/enveomics.R +1 -0
- data/utils/enveomics/Scripts/lib/enveomics_rb/anir.rb +293 -0
- data/utils/enveomics/Scripts/lib/enveomics_rb/bm_set.rb +175 -0
- data/utils/enveomics/Scripts/lib/enveomics_rb/enveomics.rb +24 -0
- data/utils/enveomics/Scripts/lib/enveomics_rb/errors.rb +17 -0
- data/utils/enveomics/Scripts/lib/enveomics_rb/gmm_em.rb +30 -0
- data/utils/enveomics/Scripts/lib/enveomics_rb/jplace.rb +253 -0
- data/utils/enveomics/Scripts/lib/enveomics_rb/match.rb +88 -0
- data/utils/enveomics/Scripts/lib/enveomics_rb/og.rb +182 -0
- data/utils/enveomics/Scripts/lib/enveomics_rb/rbm.rb +49 -0
- data/utils/enveomics/Scripts/lib/enveomics_rb/remote_data.rb +74 -0
- data/utils/enveomics/Scripts/lib/enveomics_rb/seq_range.rb +237 -0
- data/utils/enveomics/Scripts/lib/enveomics_rb/stats/rand.rb +31 -0
- data/utils/enveomics/Scripts/lib/enveomics_rb/stats/sample.rb +152 -0
- data/utils/enveomics/Scripts/lib/enveomics_rb/stats.rb +3 -0
- data/utils/enveomics/Scripts/lib/enveomics_rb/utils.rb +74 -0
- data/utils/enveomics/Scripts/lib/enveomics_rb/vcf.rb +135 -0
- data/utils/enveomics/Scripts/ogs.annotate.rb +88 -0
- data/utils/enveomics/Scripts/ogs.core-pan.rb +160 -0
- data/utils/enveomics/Scripts/ogs.extract.rb +125 -0
- data/utils/enveomics/Scripts/ogs.mcl.rb +186 -0
- data/utils/enveomics/Scripts/ogs.rb +104 -0
- data/utils/enveomics/Scripts/ogs.stats.rb +131 -0
- data/utils/enveomics/Scripts/rbm-legacy.rb +172 -0
- data/utils/enveomics/Scripts/rbm.rb +108 -0
- data/utils/enveomics/Scripts/sam.filter.rb +148 -0
- data/utils/enveomics/Tests/Makefile +10 -0
- data/utils/enveomics/Tests/Mgen_M2288.faa +3189 -0
- data/utils/enveomics/Tests/Mgen_M2288.fna +8282 -0
- data/utils/enveomics/Tests/Mgen_M2321.fna +8288 -0
- data/utils/enveomics/Tests/Nequ_Kin4M.faa +2970 -0
- data/utils/enveomics/Tests/Xanthomonas_oryzae-PilA.tribs.Rdata +0 -0
- data/utils/enveomics/Tests/Xanthomonas_oryzae-PilA.txt +7 -0
- data/utils/enveomics/Tests/Xanthomonas_oryzae.aai-mat.tsv +17 -0
- data/utils/enveomics/Tests/Xanthomonas_oryzae.aai.tsv +137 -0
- data/utils/enveomics/Tests/a_mg.cds-go.blast.tsv +123 -0
- data/utils/enveomics/Tests/a_mg.reads-cds.blast.tsv +200 -0
- data/utils/enveomics/Tests/a_mg.reads-cds.counts.tsv +55 -0
- data/utils/enveomics/Tests/alkB.nwk +1 -0
- data/utils/enveomics/Tests/anthrax-cansnp-data.tsv +13 -0
- data/utils/enveomics/Tests/anthrax-cansnp-key.tsv +17 -0
- data/utils/enveomics/Tests/hiv1.faa +59 -0
- data/utils/enveomics/Tests/hiv1.fna +134 -0
- data/utils/enveomics/Tests/hiv2.faa +70 -0
- data/utils/enveomics/Tests/hiv_mix-hiv1.blast.tsv +233 -0
- data/utils/enveomics/Tests/hiv_mix-hiv1.blast.tsv.lim +1 -0
- data/utils/enveomics/Tests/hiv_mix-hiv1.blast.tsv.rec +233 -0
- data/utils/enveomics/Tests/low-cov.bg.gz +0 -0
- data/utils/enveomics/Tests/phyla_counts.tsv +10 -0
- data/utils/enveomics/Tests/primate_lentivirus.ogs +11 -0
- data/utils/enveomics/Tests/primate_lentivirus.rbm/hiv1-hiv1.rbm +9 -0
- data/utils/enveomics/Tests/primate_lentivirus.rbm/hiv1-hiv2.rbm +8 -0
- data/utils/enveomics/Tests/primate_lentivirus.rbm/hiv1-siv.rbm +6 -0
- data/utils/enveomics/Tests/primate_lentivirus.rbm/hiv2-hiv2.rbm +9 -0
- data/utils/enveomics/Tests/primate_lentivirus.rbm/hiv2-siv.rbm +6 -0
- data/utils/enveomics/Tests/primate_lentivirus.rbm/siv-siv.rbm +6 -0
- data/utils/enveomics/build_enveomics_r.bash +45 -0
- data/utils/enveomics/enveomics.R/DESCRIPTION +31 -0
- data/utils/enveomics/enveomics.R/NAMESPACE +39 -0
- data/utils/enveomics/enveomics.R/R/autoprune.R +167 -0
- data/utils/enveomics/enveomics.R/R/barplot.R +203 -0
- data/utils/enveomics/enveomics.R/R/cliopts.R +141 -0
- data/utils/enveomics/enveomics.R/R/df2dist.R +192 -0
- data/utils/enveomics/enveomics.R/R/growthcurve.R +349 -0
- data/utils/enveomics/enveomics.R/R/prefscore.R +79 -0
- data/utils/enveomics/enveomics.R/R/recplot.R +419 -0
- data/utils/enveomics/enveomics.R/R/recplot2.R +1698 -0
- data/utils/enveomics/enveomics.R/R/tribs.R +638 -0
- data/utils/enveomics/enveomics.R/R/utils.R +90 -0
- data/utils/enveomics/enveomics.R/README.md +81 -0
- data/utils/enveomics/enveomics.R/data/growth.curves.rda +0 -0
- data/utils/enveomics/enveomics.R/data/phyla.counts.rda +0 -0
- data/utils/enveomics/enveomics.R/man/cash-enve.GrowthCurve-method.Rd +16 -0
- data/utils/enveomics/enveomics.R/man/cash-enve.RecPlot2-method.Rd +16 -0
- data/utils/enveomics/enveomics.R/man/cash-enve.RecPlot2.Peak-method.Rd +16 -0
- data/utils/enveomics/enveomics.R/man/enve.GrowthCurve-class.Rd +25 -0
- data/utils/enveomics/enveomics.R/man/enve.TRIBS-class.Rd +47 -0
- data/utils/enveomics/enveomics.R/man/enve.TRIBS.merge.Rd +23 -0
- data/utils/enveomics/enveomics.R/man/enve.TRIBStest-class.Rd +47 -0
- data/utils/enveomics/enveomics.R/man/enve.__prune.iter.Rd +26 -0
- data/utils/enveomics/enveomics.R/man/enve.__prune.reduce.Rd +26 -0
- data/utils/enveomics/enveomics.R/man/enve.__tribs.Rd +44 -0
- data/utils/enveomics/enveomics.R/man/enve.barplot.Rd +111 -0
- data/utils/enveomics/enveomics.R/man/enve.cliopts.Rd +67 -0
- data/utils/enveomics/enveomics.R/man/enve.col.alpha.Rd +34 -0
- data/utils/enveomics/enveomics.R/man/enve.col2alpha.Rd +25 -0
- data/utils/enveomics/enveomics.R/man/enve.df2dist.Rd +59 -0
- data/utils/enveomics/enveomics.R/man/enve.df2dist.group.Rd +63 -0
- data/utils/enveomics/enveomics.R/man/enve.df2dist.list.Rd +46 -0
- data/utils/enveomics/enveomics.R/man/enve.growthcurve.Rd +78 -0
- data/utils/enveomics/enveomics.R/man/enve.prefscore.Rd +50 -0
- data/utils/enveomics/enveomics.R/man/enve.prune.dist.Rd +44 -0
- data/utils/enveomics/enveomics.R/man/enve.recplot.Rd +147 -0
- data/utils/enveomics/enveomics.R/man/enve.recplot2-class.Rd +45 -0
- data/utils/enveomics/enveomics.R/man/enve.recplot2.ANIr.Rd +27 -0
- data/utils/enveomics/enveomics.R/man/enve.recplot2.Rd +77 -0
- data/utils/enveomics/enveomics.R/man/enve.recplot2.__counts.Rd +28 -0
- data/utils/enveomics/enveomics.R/man/enve.recplot2.__peakHist.Rd +24 -0
- data/utils/enveomics/enveomics.R/man/enve.recplot2.__whichClosestPeak.Rd +22 -0
- data/utils/enveomics/enveomics.R/man/enve.recplot2.changeCutoff.Rd +22 -0
- data/utils/enveomics/enveomics.R/man/enve.recplot2.compareIdentities.Rd +52 -0
- data/utils/enveomics/enveomics.R/man/enve.recplot2.coordinates.Rd +29 -0
- data/utils/enveomics/enveomics.R/man/enve.recplot2.corePeak.Rd +21 -0
- data/utils/enveomics/enveomics.R/man/enve.recplot2.extractWindows.Rd +45 -0
- data/utils/enveomics/enveomics.R/man/enve.recplot2.findPeaks.Rd +34 -0
- data/utils/enveomics/enveomics.R/man/enve.recplot2.findPeaks.__em_e.Rd +23 -0
- data/utils/enveomics/enveomics.R/man/enve.recplot2.findPeaks.__em_m.Rd +24 -0
- data/utils/enveomics/enveomics.R/man/enve.recplot2.findPeaks.__emauto_one.Rd +31 -0
- data/utils/enveomics/enveomics.R/man/enve.recplot2.findPeaks.__mow_one.Rd +56 -0
- data/utils/enveomics/enveomics.R/man/enve.recplot2.findPeaks.__mower.Rd +20 -0
- data/utils/enveomics/enveomics.R/man/enve.recplot2.findPeaks.em.Rd +51 -0
- data/utils/enveomics/enveomics.R/man/enve.recplot2.findPeaks.emauto.Rd +43 -0
- data/utils/enveomics/enveomics.R/man/enve.recplot2.findPeaks.mower.Rd +82 -0
- data/utils/enveomics/enveomics.R/man/enve.recplot2.peak-class.Rd +59 -0
- data/utils/enveomics/enveomics.R/man/enve.recplot2.seqdepth.Rd +27 -0
- data/utils/enveomics/enveomics.R/man/enve.recplot2.windowDepthThreshold.Rd +36 -0
- data/utils/enveomics/enveomics.R/man/enve.selvector.Rd +23 -0
- data/utils/enveomics/enveomics.R/man/enve.tribs.Rd +68 -0
- data/utils/enveomics/enveomics.R/man/enve.tribs.test.Rd +28 -0
- data/utils/enveomics/enveomics.R/man/enve.truncate.Rd +27 -0
- data/utils/enveomics/enveomics.R/man/growth.curves.Rd +14 -0
- data/utils/enveomics/enveomics.R/man/phyla.counts.Rd +13 -0
- data/utils/enveomics/enveomics.R/man/plot.enve.GrowthCurve.Rd +81 -0
- data/utils/enveomics/enveomics.R/man/plot.enve.TRIBS.Rd +49 -0
- data/utils/enveomics/enveomics.R/man/plot.enve.TRIBStest.Rd +48 -0
- data/utils/enveomics/enveomics.R/man/plot.enve.recplot2.Rd +125 -0
- data/utils/enveomics/enveomics.R/man/summary.enve.GrowthCurve.Rd +22 -0
- data/utils/enveomics/enveomics.R/man/summary.enve.TRIBS.Rd +22 -0
- data/utils/enveomics/enveomics.R/man/summary.enve.TRIBStest.Rd +22 -0
- data/utils/enveomics/globals.mk +8 -0
- data/utils/enveomics/manifest.json +9 -0
- data/utils/multitrim/Multitrim How-To.pdf +0 -0
- data/utils/multitrim/README.md +67 -0
- data/utils/multitrim/multitrim.py +1555 -0
- data/utils/multitrim/multitrim.yml +13 -0
- metadata +268 -6
@@ -0,0 +1,802 @@
|
|
1
|
+
{
|
2
|
+
"tasks": [
|
3
|
+
{
|
4
|
+
"task": "FastA.N50.pl",
|
5
|
+
"description": ["Calculates the N50 value of a set of sequences.",
|
6
|
+
"Alternatively, it can calculate other N** values. It also calculates",
|
7
|
+
"the total number of sequences, the total added length, and the",
|
8
|
+
"longest sequence length."],
|
9
|
+
"help_arg": "",
|
10
|
+
"see_also": ["FastA.length.pl"],
|
11
|
+
"options": [
|
12
|
+
{
|
13
|
+
"name": "Sequences",
|
14
|
+
"arg": "in_file",
|
15
|
+
"mandatory": true,
|
16
|
+
"description": "A FastA file containing the sequences."
|
17
|
+
},
|
18
|
+
{
|
19
|
+
"name": "Minimum length",
|
20
|
+
"arg": "integer",
|
21
|
+
"description": "Minimum length to take into consideration.",
|
22
|
+
"default": 0
|
23
|
+
},
|
24
|
+
{
|
25
|
+
"name": "NXX",
|
26
|
+
"arg": "integer",
|
27
|
+
"description": "Value NXX to calculate.",
|
28
|
+
"default": 50
|
29
|
+
}
|
30
|
+
]
|
31
|
+
},
|
32
|
+
{
|
33
|
+
"task": "FastA.filter.pl",
|
34
|
+
"description": "Extracts a subset of sequences from a FastA file.",
|
35
|
+
"help_arg": "-h",
|
36
|
+
"see_also": ["FastQ.filter.pl"],
|
37
|
+
"options": [
|
38
|
+
{
|
39
|
+
"opt": "-r",
|
40
|
+
"description": ["Reverse list. Extracts sequences NOT present in the",
|
41
|
+
"list."]
|
42
|
+
},
|
43
|
+
{
|
44
|
+
"opt": "-q",
|
45
|
+
"description": "Runs quietly."
|
46
|
+
},
|
47
|
+
{
|
48
|
+
"name": "List",
|
49
|
+
"arg": "in_file",
|
50
|
+
"mandatory": true,
|
51
|
+
"description": "List of sequences to extract."
|
52
|
+
},
|
53
|
+
{
|
54
|
+
"name": "Sequences",
|
55
|
+
"arg": "in_file",
|
56
|
+
"mandatory": true,
|
57
|
+
"description": "FastA file containing the superset of sequences."
|
58
|
+
},
|
59
|
+
">",
|
60
|
+
{
|
61
|
+
"name": "Subset",
|
62
|
+
"arg": "out_file",
|
63
|
+
"mandatory": true,
|
64
|
+
"description": "FastA file to be created."
|
65
|
+
}
|
66
|
+
]
|
67
|
+
},
|
68
|
+
{
|
69
|
+
"task": "FastA.filterLen.pl",
|
70
|
+
"description": "Filters a multi-FastA file by length.",
|
71
|
+
"requires": [ { "perl_lib": "Bio::SeqIO" } ],
|
72
|
+
"cite": [["Stajich et al, 2002, GRes",
|
73
|
+
"http://dx.doi.org/10.1101/gr.361602"]],
|
74
|
+
"help_arg": "",
|
75
|
+
"options": [
|
76
|
+
{
|
77
|
+
"arg": "in_file",
|
78
|
+
"mandatory": true,
|
79
|
+
"description": "Input FastA file."
|
80
|
+
},
|
81
|
+
{
|
82
|
+
"name": "Minimum length",
|
83
|
+
"arg": "integer",
|
84
|
+
"mandatory": true,
|
85
|
+
"description": "Minimum length to report a sequence."
|
86
|
+
},
|
87
|
+
">",
|
88
|
+
{
|
89
|
+
"arg": "out_file",
|
90
|
+
"mandatory": true,
|
91
|
+
"description": "Filtered FastA file."
|
92
|
+
}
|
93
|
+
]
|
94
|
+
},
|
95
|
+
{
|
96
|
+
"task": "FastA.filterN.pl",
|
97
|
+
"description": ["Filter sequences by N-content and presence of long",
|
98
|
+
"homopolymers."],
|
99
|
+
"help_arg": "",
|
100
|
+
"options": [
|
101
|
+
{
|
102
|
+
"name": "Sequences",
|
103
|
+
"arg": "in_file",
|
104
|
+
"mandatory": true,
|
105
|
+
"description": "Input file in FastA format."
|
106
|
+
},
|
107
|
+
{
|
108
|
+
"name": "Content",
|
109
|
+
"arg": "float",
|
110
|
+
"default": 0.5,
|
111
|
+
"mandatory": true,
|
112
|
+
"description": ["A number between 0 and 1 indicating the maximum",
|
113
|
+
"proportion of Ns (1 to turn off, 0.5 by default)."]
|
114
|
+
},
|
115
|
+
{
|
116
|
+
"name": "Stretch",
|
117
|
+
"arg": "integer",
|
118
|
+
"default": 100,
|
119
|
+
"description": ["A number indicating the maximum number of",
|
120
|
+
"consecutive identical nucleotides allowed (0 to turn off, 100 by",
|
121
|
+
"default)."]
|
122
|
+
},
|
123
|
+
">",
|
124
|
+
{
|
125
|
+
"name": "Filtered",
|
126
|
+
"arg": "out_file",
|
127
|
+
"mandatory": true,
|
128
|
+
"description": "Filtered set of sequences."
|
129
|
+
}
|
130
|
+
]
|
131
|
+
},
|
132
|
+
{
|
133
|
+
"task": "FastA.gc.pl",
|
134
|
+
"description": "Estimates the G+C content of sequences.",
|
135
|
+
"help_arg": "",
|
136
|
+
"options": [
|
137
|
+
{
|
138
|
+
"name": "seqs.fa",
|
139
|
+
"arg": "in_file",
|
140
|
+
"multiple_sep": " ",
|
141
|
+
"mandatory": true,
|
142
|
+
"description": "One or more FastA files."
|
143
|
+
},
|
144
|
+
">",
|
145
|
+
{
|
146
|
+
"name": "gc.txt",
|
147
|
+
"arg": "out_file",
|
148
|
+
"mandatory": true,
|
149
|
+
"description": "A table with the G+C content of the sequences."
|
150
|
+
}
|
151
|
+
]
|
152
|
+
},
|
153
|
+
{
|
154
|
+
"task": "FastA.interpose.pl",
|
155
|
+
"description": ["Interpose sequences in FastA format from two files into",
|
156
|
+
"one output file. If more than two files are provided, the script will",
|
157
|
+
"interpose all the input files."],
|
158
|
+
"see_also": ["FastA.split.rb","FastQ.interpose.pl"],
|
159
|
+
"warn": ["Please note that this script will check for the consistency of",
|
160
|
+
"the names (assuming a pair of related reads contains the same name",
|
161
|
+
"varying only in a trailing slash (/) followed by a digit. If you want",
|
162
|
+
"to turn this feature off just set the checking period to zero. If",
|
163
|
+
"you want to decrease the sampling period (to speed the script up) or",
|
164
|
+
"increase it (to make it more sensitive to errors) just change the ",
|
165
|
+
"checking period accordingly."],
|
166
|
+
"help_arg": "",
|
167
|
+
"options": [
|
168
|
+
{
|
169
|
+
"name": "Checking period",
|
170
|
+
"opt": "-T",
|
171
|
+
"arg": "integer",
|
172
|
+
"default": 1000,
|
173
|
+
"description": "Sampling period for names evaluation."
|
174
|
+
},
|
175
|
+
{
|
176
|
+
"arg": "out_file",
|
177
|
+
"mandatory": true,
|
178
|
+
"description": "Output FastA file."
|
179
|
+
},
|
180
|
+
{
|
181
|
+
"name": "Input FastA 1",
|
182
|
+
"arg": "in_file",
|
183
|
+
"mandatory": true,
|
184
|
+
"description": "First FastA file."
|
185
|
+
},
|
186
|
+
{
|
187
|
+
"name": "Input FastA 2",
|
188
|
+
"arg": "in_file",
|
189
|
+
"mandatory": true,
|
190
|
+
"description": "Second FastA file."
|
191
|
+
},
|
192
|
+
{
|
193
|
+
"name": "Additional FastA",
|
194
|
+
"arg": "in_file",
|
195
|
+
"multiple_sep": " ",
|
196
|
+
"description": "Any additional FastA files (or none)."
|
197
|
+
}
|
198
|
+
]
|
199
|
+
},
|
200
|
+
{
|
201
|
+
"task": "FastA.length.pl",
|
202
|
+
"description": "Returns the length of sequences in (multi-)FastA.",
|
203
|
+
"see_also": ["FastA.N50.pl"],
|
204
|
+
"help_arg": "",
|
205
|
+
"options": [
|
206
|
+
{
|
207
|
+
"arg": "in_file",
|
208
|
+
"multiple_sep": " ",
|
209
|
+
"mandatory": true,
|
210
|
+
"description": "One or more FastA files."
|
211
|
+
},
|
212
|
+
">",
|
213
|
+
{
|
214
|
+
"arg": "out_file",
|
215
|
+
"mandatory": true,
|
216
|
+
"description": "A table with the lengths of the sequences."
|
217
|
+
}
|
218
|
+
]
|
219
|
+
},
|
220
|
+
{
|
221
|
+
"task": "FastA.mask.rb",
|
222
|
+
"description": "Mask sequence region(s) in a FastA file.",
|
223
|
+
"help_arg": "--help",
|
224
|
+
"options": [
|
225
|
+
{
|
226
|
+
"opt": "--in",
|
227
|
+
"arg": "in_file",
|
228
|
+
"mandatory": true,
|
229
|
+
"description": "Input FastA file."
|
230
|
+
},
|
231
|
+
{
|
232
|
+
"opt": "--out",
|
233
|
+
"arg": "out_file",
|
234
|
+
"mandatory": true,
|
235
|
+
"description": "Output FastA file."
|
236
|
+
},
|
237
|
+
{
|
238
|
+
"opt": "--regions",
|
239
|
+
"arg": "string",
|
240
|
+
"mandatory": true,
|
241
|
+
"description": ["Regions to mask separated by commas.",
|
242
|
+
"Each region must be in the format \"sequence_id:from..to\"."]
|
243
|
+
},
|
244
|
+
{
|
245
|
+
"opt": "--symbol",
|
246
|
+
"arg": "string",
|
247
|
+
"default": "N",
|
248
|
+
"description": "Character used to mask the region(s)."
|
249
|
+
},
|
250
|
+
{
|
251
|
+
"opt": "--trim",
|
252
|
+
"description": ["Trim masked regions extending to the edge of a",
|
253
|
+
"sequence."]
|
254
|
+
},
|
255
|
+
{
|
256
|
+
"opt": "--wrap",
|
257
|
+
"arg": "integer",
|
258
|
+
"default": 70,
|
259
|
+
"description": ["Line length to wrap sequences. Use 0 to generate",
|
260
|
+
"1-line sequences."]
|
261
|
+
}
|
262
|
+
]
|
263
|
+
},
|
264
|
+
{
|
265
|
+
"task": "FastA.qlen.pl",
|
266
|
+
"description": ["Calculates the quartiles of the length in a set of",
|
267
|
+
"sequences. The Q2 is also known as the median. Q0 is the minimum",
|
268
|
+
"length, and Q4 is the maximum length. It also calculates TOTAL, the",
|
269
|
+
"added length of the sequences in the file, and AVG, the average",
|
270
|
+
"length."],
|
271
|
+
"help_arg": "",
|
272
|
+
"options": [
|
273
|
+
{
|
274
|
+
"name": "Sequences",
|
275
|
+
"arg": "in_file",
|
276
|
+
"mandatory": true,
|
277
|
+
"description": "A FastA file containing the sequences."
|
278
|
+
},
|
279
|
+
{
|
280
|
+
"name": "Minimum Length",
|
281
|
+
"arg": "integer",
|
282
|
+
"default": 0,
|
283
|
+
"description": "The minimum length to take into consideration."
|
284
|
+
}
|
285
|
+
]
|
286
|
+
},
|
287
|
+
{
|
288
|
+
"task": "FastA.rename.pl",
|
289
|
+
"description": "Renames a set of sequences in FastA format.",
|
290
|
+
"help_arg": "-h",
|
291
|
+
"options": [
|
292
|
+
{
|
293
|
+
"name": "Filter list",
|
294
|
+
"opt": "-f",
|
295
|
+
"description": "Ignore sequences NOT present in the list."
|
296
|
+
},
|
297
|
+
{
|
298
|
+
"name": "Quiet",
|
299
|
+
"opt": "-q",
|
300
|
+
"description": "Runs quietly."
|
301
|
+
},
|
302
|
+
{
|
303
|
+
"name": "List.txt",
|
304
|
+
"arg": "in_file",
|
305
|
+
"mandatory": true,
|
306
|
+
"description": ["Tab-delimited list of sequences, with the original",
|
307
|
+
"ID in the first column and the ID to use in the second."]
|
308
|
+
},
|
309
|
+
{
|
310
|
+
"name": "Seqs.fa",
|
311
|
+
"arg": "in_file",
|
312
|
+
"mandatory": true,
|
313
|
+
"description": "FastA file containing the superset of sequences."
|
314
|
+
},
|
315
|
+
">",
|
316
|
+
{
|
317
|
+
"name": "Renamed.fa",
|
318
|
+
"arg": "out_file",
|
319
|
+
"mandatory": true,
|
320
|
+
"description": "FastA file to be created."
|
321
|
+
}
|
322
|
+
]
|
323
|
+
},
|
324
|
+
{
|
325
|
+
"task": "FastA.revcom.pl",
|
326
|
+
"description": "Reverse-complement sequences in FastA format.",
|
327
|
+
"requires": [ { "perl_lib": "Bio::SeqIO" } ],
|
328
|
+
"cite": [["Stajich et al, 2002, GRes",
|
329
|
+
"http://dx.doi.org/10.1101/gr.361602"]],
|
330
|
+
"help_arg": "--help",
|
331
|
+
"options": [
|
332
|
+
"<",
|
333
|
+
{
|
334
|
+
"arg": "in_file",
|
335
|
+
"mandatory": true,
|
336
|
+
"description": "Input file in FastA format."
|
337
|
+
},
|
338
|
+
">",
|
339
|
+
{
|
340
|
+
"arg": "out_file",
|
341
|
+
"mandatory": true,
|
342
|
+
"description": "Output file in FastA format."
|
343
|
+
}
|
344
|
+
]
|
345
|
+
},
|
346
|
+
{
|
347
|
+
"task": "FastA.sample.rb",
|
348
|
+
"description": ["Samples a random set of sequences from a multi-FastA",
|
349
|
+
"file."],
|
350
|
+
"help_arg": "--help",
|
351
|
+
"see_also": "FastA.subsample.pl",
|
352
|
+
"options": [
|
353
|
+
{
|
354
|
+
"name": "Input File",
|
355
|
+
"opt": "--in",
|
356
|
+
"arg": "in_file",
|
357
|
+
"mandatory": true,
|
358
|
+
"description": "Input FastA file (supports .gz compression)."
|
359
|
+
},
|
360
|
+
{
|
361
|
+
"name": "Output file",
|
362
|
+
"opt": "--out",
|
363
|
+
"arg": "out_file",
|
364
|
+
"mandatory": true,
|
365
|
+
"description": "Output FastA file (supports .gz compression)."
|
366
|
+
},
|
367
|
+
{
|
368
|
+
"opt": "--fraction",
|
369
|
+
"arg": "float",
|
370
|
+
"description": ["Fraction of sequences to sample [0-1].",
|
371
|
+
"Mandatory unless Number is provided."]
|
372
|
+
},
|
373
|
+
{
|
374
|
+
"opt": "--number",
|
375
|
+
"arg": "integer",
|
376
|
+
"description": ["Number of sequences to sample.",
|
377
|
+
"Mandatory unless -f is provided."]
|
378
|
+
},
|
379
|
+
{
|
380
|
+
"opt": "--replacement",
|
381
|
+
"description": "Sample with replacement."
|
382
|
+
},
|
383
|
+
{
|
384
|
+
"opt": "--quiet",
|
385
|
+
"description": "Run quietly (no STDERR output)."
|
386
|
+
}
|
387
|
+
]
|
388
|
+
},
|
389
|
+
{
|
390
|
+
"task": "FastA.slider.pl",
|
391
|
+
"description": "Slices sequences in fixed- or variable-length windows.",
|
392
|
+
"help_arg": "-help",
|
393
|
+
"options": [
|
394
|
+
{
|
395
|
+
"opt": "-seq",
|
396
|
+
"arg": "in_file",
|
397
|
+
"mandatory": true,
|
398
|
+
"description": "Input file in FastA format."
|
399
|
+
},
|
400
|
+
{
|
401
|
+
"opt": "-out",
|
402
|
+
"arg": "out_file",
|
403
|
+
"mandatory": true,
|
404
|
+
"description": "Output file in FastA format."
|
405
|
+
},
|
406
|
+
{
|
407
|
+
"opt": "-win",
|
408
|
+
"arg": "integer",
|
409
|
+
"default": 18,
|
410
|
+
"description": "Window size."
|
411
|
+
},
|
412
|
+
{
|
413
|
+
"opt": "-step",
|
414
|
+
"arg": "integer",
|
415
|
+
"default": 1,
|
416
|
+
"description": "Step size."
|
417
|
+
},
|
418
|
+
{
|
419
|
+
"name": "Length error",
|
420
|
+
"opt": "-lerr",
|
421
|
+
"arg": "integer",
|
422
|
+
"default": 2,
|
423
|
+
"description": "Expected error in chunks length."
|
424
|
+
},
|
425
|
+
{
|
426
|
+
"opt": "-comm",
|
427
|
+
"arg": "select",
|
428
|
+
"values": [0,1],
|
429
|
+
"default": 0,
|
430
|
+
"description": ["Generate FastA comments (leaded by semi-colon) to",
|
431
|
+
"separate input sequences. Set to 0 for no-comments, to 1 for",
|
432
|
+
"comments."]
|
433
|
+
},
|
434
|
+
{
|
435
|
+
"opt": "-short",
|
436
|
+
"arg": "select",
|
437
|
+
"values": [0,1],
|
438
|
+
"default": 0,
|
439
|
+
"description": ["Use chunks shorter than the window size 'as is'.",
|
440
|
+
"Set to 0 to discard those chunks, to 1 to use them."]
|
441
|
+
}
|
442
|
+
]
|
443
|
+
},
|
444
|
+
{
|
445
|
+
"task": "FastA.split.rb",
|
446
|
+
"description": ["Evenly splits a multi-FastA file into multiple",
|
447
|
+
"multi-FastA files."],
|
448
|
+
"see_also": ["FastA.interpose.pl","FastQ.split.pl"],
|
449
|
+
"help_arg": "--help",
|
450
|
+
"options": [
|
451
|
+
{
|
452
|
+
"opt": "--input",
|
453
|
+
"arg": "in_file",
|
454
|
+
"mandatory": true,
|
455
|
+
"description": "Input FastA file."
|
456
|
+
},
|
457
|
+
{
|
458
|
+
"opt": "--prefix",
|
459
|
+
"arg": "out_file",
|
460
|
+
"mandatory": true,
|
461
|
+
"description": "Prefix of output FastA files."
|
462
|
+
},
|
463
|
+
{
|
464
|
+
"opt": "--number",
|
465
|
+
"arg": "integer",
|
466
|
+
"default": 12,
|
467
|
+
"description": "Number of output files to produce."
|
468
|
+
},
|
469
|
+
{
|
470
|
+
"opt": "--zero-padded",
|
471
|
+
"description": "Use zero-padded numbers as output index."
|
472
|
+
},
|
473
|
+
{
|
474
|
+
"opt": "--lowercase-letters",
|
475
|
+
"description": "Use lowercase letters as output index."
|
476
|
+
},
|
477
|
+
{
|
478
|
+
"name": "Output format",
|
479
|
+
"opt": "--out",
|
480
|
+
"arg": "string",
|
481
|
+
"default": "%s.%s.fa",
|
482
|
+
"description": ["Format of output filenames, where %s are replaced",
|
483
|
+
"by prefix and index."]
|
484
|
+
},
|
485
|
+
{
|
486
|
+
"opt": "--quiet",
|
487
|
+
"description": "Run quietly (no STDERR output)."
|
488
|
+
}
|
489
|
+
]
|
490
|
+
},
|
491
|
+
{
|
492
|
+
"task": "FastA.split.pl",
|
493
|
+
"description": "Splits a FastA file into two or more files.",
|
494
|
+
"warn": "Deprecated in favor of FastA.split.rb.",
|
495
|
+
"see_also": ["FastA.split.rb","FastA.interpose.pl","FastQ.split.pl"],
|
496
|
+
"help_arg": "",
|
497
|
+
"options": [
|
498
|
+
{
|
499
|
+
"arg": "in_file",
|
500
|
+
"mandatory": true,
|
501
|
+
"description": "Input file in FastA format."
|
502
|
+
},
|
503
|
+
{
|
504
|
+
"name": "Out base",
|
505
|
+
"arg": "out_file",
|
506
|
+
"mandatory": true,
|
507
|
+
"description": ["Prefix for the name of the output files. It will be",
|
508
|
+
"appended with .<i>.fa, where <i> is a consecutive number starting",
|
509
|
+
"in 1."]
|
510
|
+
},
|
511
|
+
{
|
512
|
+
"name": "Number of files",
|
513
|
+
"arg": "integer",
|
514
|
+
"default": 12,
|
515
|
+
"description": "Number of files to generate."
|
516
|
+
}
|
517
|
+
]
|
518
|
+
},
|
519
|
+
{
|
520
|
+
"task": "FastA.subsample.pl",
|
521
|
+
"description": "Subsamples a set of sequences.",
|
522
|
+
"help_arg": "-h",
|
523
|
+
"see_also": "FastA.sample.rb",
|
524
|
+
"options": [
|
525
|
+
{
|
526
|
+
"name": "Fraction",
|
527
|
+
"opt": "-f",
|
528
|
+
"arg": "string",
|
529
|
+
"default": "10",
|
530
|
+
"description": ["Fraction of the library to be sampled (as",
|
531
|
+
"percentage). It can include several values (separated by comma),",
|
532
|
+
"as well as ranges of values in the form 'from-to/by'. For",
|
533
|
+
"example, the -f value 1-5/1,10-50/10,75,99 will produce 12",
|
534
|
+
"subsamples with expected fractions 1%, 2%, 3%, 4%, 5%, 10%, 20%,",
|
535
|
+
"30%, 40%, 50%, 75%, and 99%."]
|
536
|
+
},
|
537
|
+
{
|
538
|
+
"name": "Replicates",
|
539
|
+
"opt": "-r",
|
540
|
+
"arg": "integer",
|
541
|
+
"default": 1,
|
542
|
+
"description": "Number of replicates per fraction."
|
543
|
+
},
|
544
|
+
{
|
545
|
+
"name": "Out base",
|
546
|
+
"opt": "-o",
|
547
|
+
"arg": "out_file",
|
548
|
+
"description": ["Prefix of the output files to be created. The",
|
549
|
+
"output files will have a suffix of the form",
|
550
|
+
"'.fraction-replicate.fa', where 'fraction' is the percentage",
|
551
|
+
"sampled and 'replicate' is an increasing integer for replicates",
|
552
|
+
"of the same fraction. By default: Path to the input file."]
|
553
|
+
},
|
554
|
+
{
|
555
|
+
"name": "Force",
|
556
|
+
"opt": "-F",
|
557
|
+
"description": "Force overwriting output file(s)."
|
558
|
+
},
|
559
|
+
{
|
560
|
+
"name": "Zeroes",
|
561
|
+
"opt": "-z",
|
562
|
+
"description": ["Include leading zeroes in the numeric parts of the",
|
563
|
+
"output files (e.g., file.002.50-01.fa instead of file.2.50-1.fa),",
|
564
|
+
"so that alphabetic sorting of files reflects the sampled",
|
565
|
+
"fraction."]
|
566
|
+
},
|
567
|
+
{
|
568
|
+
"name": "Quiet",
|
569
|
+
"opt": "-q",
|
570
|
+
"description": "Run quietly."
|
571
|
+
},
|
572
|
+
{
|
573
|
+
"arg": "in_file",
|
574
|
+
"mandatory": true,
|
575
|
+
"multiple_sep": " ",
|
576
|
+
"description": "Input multi-FastA file(s)."
|
577
|
+
}
|
578
|
+
]
|
579
|
+
},
|
580
|
+
{
|
581
|
+
"task": "FastA.tag.rb",
|
582
|
+
"description": "Generates easy-to-parse tagged reads from FastA files.",
|
583
|
+
"see_also": ["FastQ.tag.rb"],
|
584
|
+
"help_arg": "--help",
|
585
|
+
"options": [
|
586
|
+
{
|
587
|
+
"name": "Input file",
|
588
|
+
"opt": "--in",
|
589
|
+
"arg": "in_file",
|
590
|
+
"mandatory": true,
|
591
|
+
"description": "FastA file containing the sequences."
|
592
|
+
},
|
593
|
+
{
|
594
|
+
"name": "Output file",
|
595
|
+
"opt": "--out",
|
596
|
+
"arg": "out_file",
|
597
|
+
"mandatory": true,
|
598
|
+
"description": "FastA file to create."
|
599
|
+
},
|
600
|
+
{
|
601
|
+
"opt": "--prefix",
|
602
|
+
"arg": "string",
|
603
|
+
"description": "Prefix to use in all IDs."
|
604
|
+
},
|
605
|
+
{
|
606
|
+
"opt": "--suffix",
|
607
|
+
"arg": "string",
|
608
|
+
"description": "Suffix to use in all IDs."
|
609
|
+
},
|
610
|
+
{
|
611
|
+
"opt": "--defline",
|
612
|
+
"description": "Keep the original defline after a space."
|
613
|
+
},
|
614
|
+
{
|
615
|
+
"opt": "--list",
|
616
|
+
"arg": "in_file",
|
617
|
+
"description": "Reads a list of IDS."
|
618
|
+
},
|
619
|
+
{
|
620
|
+
"opt": "--quiet",
|
621
|
+
"description": "Run quietly (no STDERR output)."
|
622
|
+
}
|
623
|
+
]
|
624
|
+
},
|
625
|
+
{
|
626
|
+
"task": "FastA.per_file.pl",
|
627
|
+
"description": ["Extracts all the sequences in a multi-FastA into",
|
628
|
+
"multiple single-FastA files."],
|
629
|
+
"see_also": "FastA.split.rb",
|
630
|
+
"help_arg": "",
|
631
|
+
"options": [
|
632
|
+
{
|
633
|
+
"arg": "out_dir",
|
634
|
+
"mandatory": true,
|
635
|
+
"description": "Output directory for the individual files."
|
636
|
+
},
|
637
|
+
{
|
638
|
+
"arg": "in_file",
|
639
|
+
"mandatory": true,
|
640
|
+
"multiple_sep": " ",
|
641
|
+
"description": "Input multi-FastA file(s)."
|
642
|
+
}
|
643
|
+
]
|
644
|
+
},
|
645
|
+
{
|
646
|
+
"task": "FastA.extract.rb",
|
647
|
+
"description": ["Extracts a list of sequences and/or coordinates from",
|
648
|
+
"multi-FastA files."],
|
649
|
+
"help_arg": "--help",
|
650
|
+
"options": [
|
651
|
+
{
|
652
|
+
"name": "Input file",
|
653
|
+
"opt": "--in",
|
654
|
+
"arg": "in_file",
|
655
|
+
"mandatory": true,
|
656
|
+
"description": "Input FastA file."
|
657
|
+
},
|
658
|
+
{
|
659
|
+
"name": "Output file",
|
660
|
+
"opt": "--out",
|
661
|
+
"arg": "out_file",
|
662
|
+
"mandatory": true,
|
663
|
+
"description": "Output FastA file."
|
664
|
+
},
|
665
|
+
{
|
666
|
+
"name": "Coordinates",
|
667
|
+
"opt": "--coords",
|
668
|
+
"arg": "string",
|
669
|
+
"description": ["Comma-delimited list of coordinates (mandatory",
|
670
|
+
"unless -C is passed).",
|
671
|
+
"The format of the coordinates is SEQ:FROM..TO or SEQ:FROM~LEN:",
|
672
|
+
"SEQ: Sequence ID, or * (asterisk) to extract range from all",
|
673
|
+
"sequences",
|
674
|
+
"FROM: Integer, position of the first base to include (can be",
|
675
|
+
"negative)",
|
676
|
+
"TO: Integer, last base to include (can be negative)",
|
677
|
+
"LEN: Length of the range to extract."]
|
678
|
+
},
|
679
|
+
{
|
680
|
+
"name": "Coordinates file",
|
681
|
+
"opt": "--coords-file",
|
682
|
+
"arg": "in_file",
|
683
|
+
"description": ["File containing the coordinates, one per line.",
|
684
|
+
"Each line must follow the format described for Coordinates."]
|
685
|
+
},
|
686
|
+
{
|
687
|
+
"opt": "--quiet",
|
688
|
+
"description": "Run quietly (no STDERR output)."
|
689
|
+
}
|
690
|
+
]
|
691
|
+
},
|
692
|
+
{
|
693
|
+
"task": "FastA.fragment.rb",
|
694
|
+
"description": ["Simulates incomplete (fragmented) drafts from complete",
|
695
|
+
"genomes."],
|
696
|
+
"help_arg": "--help",
|
697
|
+
"options": [
|
698
|
+
{
|
699
|
+
"name": "Input file",
|
700
|
+
"opt": "--in",
|
701
|
+
"arg": "in_file",
|
702
|
+
"mandatory": true,
|
703
|
+
"description": "FastA file containing the complete sequences."
|
704
|
+
},
|
705
|
+
{
|
706
|
+
"name": "Output file",
|
707
|
+
"opt": "--out",
|
708
|
+
"arg": "out_file",
|
709
|
+
"mandatory": true,
|
710
|
+
"description": "FastA to create."
|
711
|
+
},
|
712
|
+
{
|
713
|
+
"opt": "--completeness",
|
714
|
+
"arg": "float",
|
715
|
+
"mandatory": true,
|
716
|
+
"description": ["Fraction of genome completeness to simulate from 0",
|
717
|
+
"to 1."]
|
718
|
+
},
|
719
|
+
{
|
720
|
+
"name": "Minimum length",
|
721
|
+
"opt": "--minlen",
|
722
|
+
"arg": "integer",
|
723
|
+
"description": "Minimum fragment length to report.",
|
724
|
+
"default": 500
|
725
|
+
},
|
726
|
+
{
|
727
|
+
"opt": "--sorted",
|
728
|
+
"description": ["Keep fragments sorted as in the input file. By",
|
729
|
+
"default, fragments are shuffled."]
|
730
|
+
},
|
731
|
+
{
|
732
|
+
"opt": "--quiet",
|
733
|
+
"description": "Run quietly (no STDERR output)."
|
734
|
+
}
|
735
|
+
]
|
736
|
+
},
|
737
|
+
{
|
738
|
+
"task": "FastA.toFastQ.rb",
|
739
|
+
"description": "Creates a FastQ-compliant file from a FastA file.",
|
740
|
+
"see_also": "FastQ.toFastA.awk",
|
741
|
+
"help_arg": "--help",
|
742
|
+
"options": [
|
743
|
+
{
|
744
|
+
"name": "Input FastA",
|
745
|
+
"opt": "--in",
|
746
|
+
"arg": "in_file",
|
747
|
+
"mandatory": true,
|
748
|
+
"description": "Input FastA file (supports .gz compression)."
|
749
|
+
},
|
750
|
+
{
|
751
|
+
"name": "Output FastQ",
|
752
|
+
"opt": "--out",
|
753
|
+
"arg": "out_file",
|
754
|
+
"mandatory": true,
|
755
|
+
"description": "Output FastQ file (supports .gz compression)."
|
756
|
+
},
|
757
|
+
{
|
758
|
+
"opt": "--quality",
|
759
|
+
"arg": "integer",
|
760
|
+
"default": 31,
|
761
|
+
"description": ["PHRED quality score to use (fixed), in the range",
|
762
|
+
"[-5, 41]."]
|
763
|
+
},
|
764
|
+
{
|
765
|
+
"opt": "--encoding",
|
766
|
+
"arg": "integer",
|
767
|
+
"default": 33,
|
768
|
+
"description": "Base encoding (33 or 64)."
|
769
|
+
}
|
770
|
+
]
|
771
|
+
},
|
772
|
+
{
|
773
|
+
"task": "FastA.wrap.rb",
|
774
|
+
"description": "Wraps sequences in a FastA to a given line length.",
|
775
|
+
"help_arg": "--help",
|
776
|
+
"options": [
|
777
|
+
{
|
778
|
+
"name": "Input FastA",
|
779
|
+
"opt": "--in",
|
780
|
+
"arg": "in_file",
|
781
|
+
"mandatory": true,
|
782
|
+
"description": "Input FastA file."
|
783
|
+
},
|
784
|
+
{
|
785
|
+
"name": "Output FastA",
|
786
|
+
"opt": "--out",
|
787
|
+
"arg": "out_file",
|
788
|
+
"mandatory": true,
|
789
|
+
"description": "Output FastA file."
|
790
|
+
},
|
791
|
+
{
|
792
|
+
"name": "Wrap length",
|
793
|
+
"opt": "--wrap",
|
794
|
+
"arg": "integer",
|
795
|
+
"description": ["Line length to wrap sequences. Use 0 to generate",
|
796
|
+
"1-line sequences."],
|
797
|
+
"default": 70
|
798
|
+
}
|
799
|
+
]
|
800
|
+
}
|
801
|
+
]
|
802
|
+
}
|