miga-base 1.2.17.0 → 1.2.17.1

data/utils/FastAAI/FastAAI

@@ -0,0 +1,3659 @@
+#!/usr/bin/env python3
+
+################################################################################
+"""---0.0 Import Modules---"""
+import subprocess 
+import argparse 
+import datetime 
+import shutil
+import textwrap
+import multiprocessing
+import pickle
+import gzip
+import tempfile
+#Shouldn't play any role.
+#from random import randint
+
+#We could probably remove Path, too.
+from pathlib import Path
+#This as well
+from functools import partial
+import time
+from collections import defaultdict
+import sys
+import os
+from math import floor
+import sqlite3
+#numpy dependency
+import numpy as np
+import io
+import random
+
+
+#Takes a bytestring from the SQL database and converts it to a numpy array.
+def convert_array(bytestring):
+	return np.frombuffer(bytestring, dtype = np.int32)
+
+def convert_float_array_16(bytestring):
+	return np.frombuffer(bytestring, dtype = np.float16)
+
+def convert_float_array_32(bytestring):
+	return np.frombuffer(bytestring, dtype = np.float32)
+	
+def convert_float_array_64(bytestring):
+	return np.frombuffer(bytestring, dtype = np.float64)
+
+	
+#Iterator for agnostic reader
+class agnostic_reader_iterator:
+	def __init__(self, reader):
+		self.handle_ = reader.handle
+		self.is_gz_ = reader.is_gz
+		
+	def __next__(self):
+		if self.is_gz_:
+			line = self.handle_.readline().decode()
+		else:
+			line = self.handle_.readline()
+		
+		#Ezpz EOF check
+		if line:
+			return line
+		else:
+			raise StopIteration
+
+#File reader that doesn't care if you give it a gzipped file or not.
+class agnostic_reader:
+	def __init__(self, file):
+		self.path = file
+		
+		with open(file, 'rb') as test_gz:
+			#Gzip magic number
+			is_gz = (test_gz.read(2) == b'\x1f\x8b')
+		
+		self.is_gz = is_gz
+		
+		if is_gz:
+			self.handle = gzip.open(self.path)
+		else:
+			self.handle = open(self.path)
+			
+	def __iter__(self):
+		return agnostic_reader_iterator(self)
+		
+	def close(self):
+		self.handle.close()
+
+#FastAAI database class. This is the final database
+class fastaai_database:
+	def __init__(self, path):
+		#open SQL db and load in
+		
+		self.path = path
+		self.exists = os.path.exists(path)
+		
+		self.child = None
+		self.connection = None
+		self.cursor = None
+		
+		self.child_connection = None
+		self.child_cursor = None
+		
+		self.accessions = None
+		#self.genomes = None
+		
+		#gak stands for 'genome_accession_kmer_counts'
+		self.gak = None
+		self.genome_index = None
+		#Go from index to name
+		self.reverse_genome_index = None
+		self.protein_counts_by_genome = None
+		
+		#self.accession_set = None
+		
+		self.verbosity = False
+		
+	#Open an SQL connection
+	def activate_connection(self, with_converter = True):
+		# Converts np.array to TEXT when inserting
+		##sqlite3.register_adapter(np.ndarray, adapt_array)
+
+		#Converts byte string to numpy ndarray(int32) upon read from DB.
+		if with_converter:
+			sqlite3.register_converter("array", convert_array)
+			self.connection = sqlite3.connect(self.path, detect_types=sqlite3.PARSE_DECLTYPES)
+			
+		else:
+			#sqlite3.register_converter("array", convert_array)
+			self.connection = sqlite3.connect(self.path)
+			
+		self.cursor = self.connection.cursor()
+		self.exists = True
+	
+	#Close an SQL connection
+	def close_connection(self):
+		self.cursor.close()
+		self.connection.close()
+		#True cleanup - even a closed SQL connection obj cannot be passed to multiple processors, but a nonetype can.
+		self.cursor = None
+		self.connection = None
+		
+	def initialize_parent_database(self):
+		if not self.exists:
+			print("I need to be activated first!")
+		else:
+			#DB exists. Add metadata tables if needed.
+			self.cursor.execute(''' SELECT count(name) FROM sqlite_master WHERE type='table' AND name='genome_index' ''')
+			if self.cursor.fetchone()[0]!=1 : 
+				self.cursor.execute('''CREATE TABLE genome_index
+				   (genome text, gen_id INTEGER PRIMARY KEY, protein_count INTEGER)''')
+				self.connection.commit()
+				
+			self.cursor.execute(''' SELECT count(name) FROM sqlite_master WHERE type='table' AND name='genome_acc_kmer_counts' ''')
+			if self.cursor.fetchone()[0]!=1 : 
+				self.cursor.execute('''CREATE TABLE genome_acc_kmer_counts
+				   (genome INTEGER, accession INTEGER, count INTEGER)''')
+				self.connection.commit()
+				
+	#Access an existing master database
+	def activate_child_connection(self, child):
+		#Don't try to connect unless it exists. This should never fail.
+		if os.path.exists(child):
+			self.child = child
+			self.child_connection = sqlite3.connect(self.child, detect_types=sqlite3.PARSE_DECLTYPES)
+			self.child_cursor = self.child_connection.cursor()
+		else:
+			print("Child database:", child, "not found!")
+			
+	#Close access to master DB
+	def close_child_connection(self):
+		if self.child_cursor is not None:
+			self.child_cursor.close()
+			self.child_connection.close()
+			self.child_cursor = None
+			self.child_connection = None
+			self.child = None
+				
+	def add_child_to_parent(self, acc, child_db, remove = True, selected_kmers = None, genomes_too = False, just_genomes = False, update_gak = False):
+		accession_index = generate_accessions_index()
+		
+		create_command = "CREATE TABLE IF NOT EXISTS " + acc + " (kmer INTEGER PRIMARY KEY, genomes array)"
+		
+		if not just_genomes:
+			self.cursor.execute(create_command)
+			self.connection.commit()
+		
+		if genomes_too or just_genomes:
+			create_command = "CREATE TABLE IF NOT EXISTS " + acc + "_genomes (genome INTEGER PRIMARY KEY, kmers array)"
+			self.cursor.execute(create_command)
+			self.connection.commit()
+		
+		attach = "attach '"+child_db+"' as toMerge"
+		
+		if selected_kmers is not None:
+			add = "INSERT OR REPLACE INTO " + acc + " SELECT * FROM toMerge." + acc + " WHERE kmer in ({kmers})".format(kmers = ','.join(['?']*len(selected_kmers)))
+		else:
+			add = "INSERT OR REPLACE INTO " + acc + " SELECT * FROM toMerge." + acc
+		
+		if genomes_too or just_genomes:
+			add_genomes = "INSERT OR REPLACE INTO " + acc + "_genomes" + " SELECT * FROM toMerge." + acc+"_genomes"
+			if update_gak:
+				sql_acc_num = acc.replace("_", ".")
+				sql_acc_num = accession_index[sql_acc_num]
+				#Return num bytes, which is always 4*as many as there are entries, as the dtype is int32. See unique_kmers.
+				gak_sql = 'INSERT OR REPLACE INTO genome_acc_kmer_counts SELECT genome, ' + str(sql_acc_num) + ', length(kmers)/4 FROM toMerge.' + acc + '_genomes'
+		
+		detach = "detach toMerge"
+		
+		self.cursor.execute(attach)
+		self.connection.commit()
+		
+		if not just_genomes:
+			if selected_kmers is not None:
+				self.cursor.execute(add, selected_kmers)
+			else:
+				self.cursor.execute(add)
+			
+			self.connection.commit()	
+		
+		if genomes_too or just_genomes:
+			self.cursor.execute(add_genomes)
+			self.connection.commit()
+			if update_gak:
+				self.cursor.execute(gak_sql)
+				self.connection.commit()
+				
+		self.cursor.execute(detach)
+		self.connection.commit()
+		
+		if remove:
+			os.remove(child_db)
+
+	def add_genomes_first(self, accession, kmer_dict):
+		kmer_lists = []
+		for genome in kmer_dict:
+			kmer_lists.append((genome, kmer_dict[genome].tobytes()))
+			
+	
+		sql_friendly_accession = accession.replace(".", "_")
+		
+		#self.cursor.execute(" DROP TABLE IF EXISTS " + sql_friendly_accession + "_genomes")
+		
+		self.cursor.execute("CREATE TABLE IF NOT EXISTS " + sql_friendly_accession + "_genomes (genome INTEGER PRIMARY KEY, kmers array)")
+		self.connection.commit()
+		
+		self.cursor.executemany("INSERT OR REPLACE INTO " + sql_friendly_accession + "_genomes VALUES (?, ?) ", kmer_lists)
+			
+		self.connection.commit()
+		
+		return sql_friendly_accession
+		
+			
+	def load_genome_index(self):		
+		self.genome_index = {}
+		self.reverse_genome_index = {}
+		self.protein_counts_by_genome = {}
+		
+		sql_command = ("SELECT genome, gen_id, protein_count FROM genome_index")
+		
+		#Break resist.
+		gen = None
+		id = None
+		protein_count = None
+		
+		for result in self.cursor.execute(sql_command).fetchall():
+			gen = result[0]
+			id = result[1]
+			protein_count = result[2]
+			
+			self.genome_index[gen] = id
+			self.reverse_genome_index[id] = gen
+			self.protein_counts_by_genome[id] = protein_count
+				
+		del gen
+		del id
+		del protein_count
+		
+	def load_accessions(self, permitted_genomes = None, permitted_accessions = None):		
+		#self.protein_counts_by_genome = None
+		
+		self.gak = defaultdict(lambda: defaultdict())
+		self.accessions = set()
+				
+				
+		#It's possible to do both of these. Don't.
+		if permitted_genomes is not None:
+			sql_command = "SELECT * FROM genome_acc_kmer_counts WHERE genome IN ({genomes})".format(genomes=','.join(['?']*len(permitted_genomes)))
+			#data type is very important to SQL
+			sql_friendly = [int(permitted_genomes[i]) for i in range(0, len(permitted_genomes))]
+			for result in self.cursor.execute(sql_command, sql_friendly).fetchall():
+				genome, accession, kmer_ct = result[0], result[1], result[2]
+				self.gak[genome][accession] = kmer_ct
+				
+		if permitted_accessions is not None:
+			sql_command = "SELECT * FROM genome_acc_kmer_counts WHERE accession IN ({accessions})".format(accessions=','.join(['?']*len(permitted_accessions)))
+			#data type is very important to SQL
+			#sql_friendly = [int(permitted_accessions[i]) for i in range(0, len(permitted_genomes))]
+			for result in self.cursor.execute(sql_command, permitted_accessions).fetchall():
+				genome, accession, kmer_ct = result[0], result[1], result[2]
+				self.gak[genome][accession] = kmer_ct
+				
+		#Normal case
+		if permitted_accessions is None and permitted_genomes is None:
+			sql_command = "SELECT * FROM genome_acc_kmer_counts"
+			for result in self.cursor.execute(sql_command).fetchall():
+				genome, accession, kmer_ct = result[0], result[1], result[2]
+				self.gak[genome][accession] = kmer_ct
+
+		#un-defaultdict
+		self.gak = dict(self.gak)
+		for genome in self.gak:
+			self.gak[genome] = dict(self.gak[genome])
+			self.accessions = self.accessions.union(self.gak[genome].keys())
+			
+		self.accessions = tuple(self.accessions)
+		
+	def just_accessions(self):
+		converter = generate_accessions_index()
+		acc_sql = "SELECT name FROM sqlite_master WHERE type='table'"
+		tables = [item[0] for item in self.cursor.execute(acc_sql).fetchall()]
+		
+		genome_tables = []
+		for table in tables:
+			if table.endswith('_genomes'):
+				genome_tables.append(table)
+		
+		for table in genome_tables:
+			tables.pop(tables.index(table))
+
+		tables.pop(tables.index('genome_acc_kmer_counts'))
+		tables.pop(tables.index('genome_index'))
+		
+		#Back to indicies.
+		tables = [converter[table.replace('_', '.')] for table in tables]
+		
+		self.accessions = tuple(tables)	
+		
+	def unload_genomes_and_accessions(self):
+		self.gak = None
+		self.genome_index = None
+		#Go from index to name
+		self.reverse_genome_index = None
+		self.protein_counts_by_genome = None
+		
+#Child database class. This is only used during database builds and merges. Designed to take one single accession at a time and produce a correctly formatted table of kmers and accessions.
+class child_database:
+	def __init__(self, path, parent):
+		#open SQL db and load in
+		
+		self.path = path
+		self.exists = False
+		
+		self.parent = parent
+		self.parent_exists = os.path.exists(parent)
+		
+		self.connection = None
+		self.cursor = None
+		
+		self.parent_connection = None
+		self.parent_cursor = None
+		
+		self.verbosity = False
+		
+	#Open an SQL connection
+	def activate_child_connection(self):
+		# Converts np.array to TEXT when inserting
+		##sqlite3.register_adapter(np.ndarray, adapt_array)
+
+		# Converts TEXT to np.array when selecting
+		sqlite3.register_converter("array", convert_array)
+		
+		self.connection = sqlite3.connect(self.path, detect_types=sqlite3.PARSE_DECLTYPES)
+		self.cursor = self.connection.cursor()
+		self.exists = True
+	
+	#Close an SQL connection
+	def close_child_connection(self):
+		self.cursor.close()
+		self.connection.close()
+		#True cleanup - even a closed SQL connection obj cannot be passed to multiple processors, but a nonetype can.
+		self.cursor = None
+		self.connection = None
+		
+	def initialize_child_database(self):
+		if not self.exists:
+			print("I need to be activated first!")
+		else:
+			#DB exists. Add metadata tables.
+			self.cursor.execute(''' SELECT count(name) FROM sqlite_master WHERE type='table' AND name='genome_index' ''')
+			if self.cursor.fetchone()[0]!=1 : 
+				self.cursor.execute('''CREATE TABLE genome_index
+				   (genome text, gen_id integer, protein_count integer)''')
+				self.connection.commit()
+				
+			self.cursor.execute(''' SELECT count(name) FROM sqlite_master WHERE type='table' AND name='genome_acc_kmer_counts' ''')
+			if self.cursor.fetchone()[0]!=1 : 
+				self.cursor.execute('''CREATE TABLE genome_acc_kmer_counts
+				   (genome integer, accession integer, count integer)''')
+				self.connection.commit()
+				
+	
+	#Access an existing master database
+	def activate_parent_connection(self):
+		if os.path.exists(self.parent):
+			self.parent_exists = True
+			#sqlite3.register_adapter(np.ndarray, adapt_array)
+			# Converts TEXT to np.array when selecting
+			sqlite3.register_converter("array", convert_array)
+			self.parent_connection = sqlite3.connect(self.parent, detect_types=sqlite3.PARSE_DECLTYPES)
+			self.parent_cursor = self.parent_connection.cursor()
+	
+	#Close access to master DB
+	def close_parent_connection(self):
+		if self.parent_cursor is not None:
+			self.parent_cursor.close()
+			self.parent_connection.close()
+			self.parent_cursor = None
+			self.parent_connection = None
+	
+	def add_genomes_first(self, accession, kmer_lists):
+	
+		#kmer_lists = []
+		#Shoot... gotta pass the args
+		
+		#for file in prepared_files:
+		#	if accession in file.best_hits_kmers:
+		#		kmer_lists.append((genome_index[file.basename], file.best_hits_kmers[accession].tobytes()))
+	
+		sql_friendly_accession = accession.replace(".", "_")
+		
+		self.cursor.execute(" DROP TABLE IF EXISTS " + sql_friendly_accession + "_genomes")
+		
+		self.cursor.execute("CREATE TABLE " + sql_friendly_accession + "_genomes (genome INTEGER PRIMARY KEY, kmers array)")
+		self.connection.commit()
+		
+		self.cursor.executemany(" INSERT INTO " + sql_friendly_accession + "_genomes VALUES (?, ?) ", kmer_lists)
+			
+		self.connection.commit()
+		
+		return sql_friendly_accession
+		
+	
+	def add_accession(self, accession, insert_kmers):
+		sql_friendly_accession = accession.replace(".", "_")
+		
+		if self.parent_exists:
+			parent_kmers = {}
+			#Check to see if this acc. is already in parent DB
+			table_exists = (self.parent_cursor.execute(" SELECT count(name) FROM sqlite_master WHERE type='table' AND name=(?)", (sql_friendly_accession,)).fetchone()[0] == 1)
+			#If the accession is in the parent DB
+			if table_exists:
+				#Select the records where the kmers are in the new kmers to be added - we don't have to modify the ones that aren't.
+				search_command = "SELECT * FROM "+ sql_friendly_accession + " WHERE kmer IN ({kmers})".format(kmers=','.join(['?']*len(insert_kmers)))
+				
+				#Convert the kmers in the current insert list to the correct type for sql to match them
+				selection = tuple([int(key) for key in insert_kmers.keys()])
+				
+				for item in self.parent_cursor.execute(search_command, selection).fetchall():
+					#Get the kmer for this parent
+					k = item[0]
+					#If the record would be modified in the parent, combine the to-add (which will replace the row) with the existing data. Otw. the record is unaffected and we can ignore it.
+					if k in insert_kmers:
+						insert_kmers[k] = np.union1d(insert_kmers[k], item[1])
+			
+			
+			#Free up the space.
+			del parent_kmers
+		
+		formatted_kmers = []
+		
+		#Translate the ndarray into its constituent byte data
+		for kmer in insert_kmers:
+			formatted_kmers.append((int(kmer), insert_kmers[kmer].tobytes(), ))
+			
+		del insert_kmers
+			
+		#Remove the child if it exists - it shouldn't ever exist because these child DBs should be deleted upon being added to the parent, but might if a run was stopped halfway.
+		self.cursor.execute(" DROP TABLE IF EXISTS " + sql_friendly_accession)
+		
+		self.cursor.execute("CREATE TABLE " + sql_friendly_accession + " (kmer INTEGER PRIMARY KEY, genomes array)")
+		self.connection.commit()
+		
+		self.cursor.executemany(" INSERT INTO " + sql_friendly_accession + " VALUES (?, ?) ", formatted_kmers)
+			
+		self.connection.commit()
+			
+		del formatted_kmers
+		
+		return sql_friendly_accession
+	
+	
+#Holds partial results for calculating AAI.
+class calculation_database:
+	def __init__(self, path, precision):
+		#open SQL db and load in
+		
+		self.path = path
+		self.exists = False
+
+		self.connection = None
+		self.cursor = None
+		
+		self.genomes = None
+
+		self.verbosity = False
+		
+		self.precision = precision
+		
+	#Open an SQL connection
+	def activate_connection(self):
+		# Converts np.array to TEXT when inserting
+		##sqlite3.register_adapter(np.ndarray, adapt_array)
+
+		# Converts TEXT to np.array when selecting
+		if self.precision == "low":
+			sqlite3.register_converter("array", convert_float_array_16)
+		if self.precision == "med":
+			sqlite3.register_converter("array", convert_float_array_32)
+		if self.precision == "high":
+			sqlite3.register_converter("array", convert_float_array_64)
+		
+		self.connection = sqlite3.connect(self.path, detect_types=sqlite3.PARSE_DECLTYPES)
+		self.cursor = self.connection.cursor()
+		self.exists = True
+	
+	#Close an SQL connection
+	def close_connection(self):
+		self.cursor.close()
+		self.connection.close()
+		#True cleanup - even a closed SQL connection obj cannot be passed to multiple processors, but a nonetype can.
+		self.cursor = None
+		self.connection = None
+		
+	def initialize_database(self):
+		if not self.exists:
+			print("I need to be activated first!")
+		else:
+			#DB exists. Add metadata tables.
+			self.cursor.execute("DROP TABLE IF EXISTS jaccards")
+			self.connection.commit()
+			self.cursor.execute("CREATE TABLE jaccards (genome INTEGER PRIMARY KEY, jaccards array)")
+			self.connection.commit()
+	
+'''
+Class for handling all of the raw genome/protein/protein+HMM file inputs when building a database.
+
+Takes a file or files and processes them from genome -> protein, protein -> hmm, prot+HMM -> kmerized protein best hits as numpy int arrays according to the kmer_index
+
+'''
+class input_file:
+	def __init__(self, input_path, output, verbosity):
+		#starting path for the file; irrelevant for protein and hmm, but otherwise useful for keeping track.
+		self.path = input_path
+		#Output directory starts with this
+		self.output = os.path.normpath(os.path.basename(output) + "/")
+		#For printing file updates, this is the input name
+		self.name = os.path.basename(input_path)
+		#original name is the key used for the genomes index later on.
+		self.original_name = os.path.basename(input_path)
+		#This is the name that can be used for building files with new extensions.
+		if input_path.endswith(".gz"):
+			#Remove .gz first to make names consistent.
+			self.basename = os.path.splitext(os.path.basename(input_path[:-3]))[0]
+		else:
+			self.basename = os.path.splitext(os.path.basename(input_path))[0]
+		#'genome' or 'protein' or 'protein and HMM' 
+		self.status = None
+		#These will keep track of paths for each stage of file for us.
+		self.genome = None
+		self.protein = None
+		self.hmm = None
+		
+		self.best_hits = None
+		self.best_hits_kmers = None
+		
+		self.protein_count = 0
+		self.protein_kmer_count = {}
+		
+		self.trans_table = None
+		self.start_time = None
+		self.end_time = None
+		self.err_log = ""
+		#doesn't get updated otw.
+		self.initial_state = "protein+HMM"
+		
+		self.verbose = verbosity
+		
+		#r_scripts_loc = os.path.dirname(sys.modules['metapop'].__file__) + "/metapop_r/"
+		#"00.Libraries/01.SCG_HMMs/Complete_SCG_DB.hmm"
+		self.hmm_path = None
+		try:
+			#Try to locate the data bundled as it would be with a pip/conda install.
+			script_path = os.path.dirname(sys.modules['fastAAI_HMM_models'].__file__)
+			hmm_complete_model = script_path + '/00.Libraries/01.SCG_HMMs/Complete_SCG_DB.hmm'
+			self.hmm_path = str(hmm_complete_model)
+			#Check that the file exists or fail to the except.
+			fh = open(self.hmm_path)
+			fh.close()
+		except:
+			#Look in the same dir as the script; old method/MiGA friendly
+			script_path = Path(__file__)
+			script_dir = script_path.parent
+			hmm_complete_model = script_dir / "00.Libraries/01.SCG_HMMs/Complete_SCG_DB.hmm"
+			self.hmm_path = str(hmm_complete_model)
+	
+	#Functions for externally setting status and file paths of particular types
+	def set_genome(self, path):
+		self.status = 'genome'
+		self.genome = path
+	
+	def set_protein(self, path):
+		self.status = 'protein'
+		self.protein = path
+
+	def set_hmm(self, path):
+		if self.protein is None:
+			print("Warning! I don't have a protein yet, so this HMM will be useless to me until I do!")
+		self.status = 'protein and hmm'
+		self.hmm = path
+
+	#Runs prodigal, compares translation tables and stores faa files
+	def genome_to_protein(self):
+		if self.genome is None:
+			print(self.name, "wasn't a declared as a genome! I can't make this into a protein!")
+		else:
+			folder = Path(self.output + "/predicted_proteins")
+			protein_output = folder / (self.basename + '.faa')
+			output_11 = folder / (self.basename + '.faa.11')
+			output_4 = folder / (self.basename + '.faa.4')
+			temp_output = folder / (self.basename + '.temp')
+			
+			intermediate = folder / (self.basename + '_genome_intermediate.fasta')
+			
+			#total_bases = 0
+			
+			genome_parser = agnostic_reader(self.genome)
+			
+			if genome_parser.is_gz:
+				#File was a gzip; decompress it to an intermediate file and then run prodigal; delete after
+				#print("unzipping input...")
+				midpoint = open(intermediate, "w")
+				#Count input bases and write an unzipped file for prodigal's sake.
+				for line in genome_parser:
+					#if not line.startswith(">"):
+					#	total_bases += len(line.strip())
+					midpoint.write(line)
+					
+				midpoint.close()
+				
+			else:
+				#File is already unzipped, just point to it
+				intermediate = self.genome
+				#Count input bases
+				#for line in genome_parser:
+				#	if not line.startswith(">"):
+				#		total_bases += len(line.strip())
+			
+			genome_parser.close()
+			'''
+			A chunk of code originally indended to match GTDBtk's table selection criteria.
+			if total_bases > 100000:
+				#training mode
+				subprocess.call(["prodigal", "-i", str(intermediate), "-a", str(output_11), "-q", "-o", str(temp_output)])				
+				subprocess.call(["prodigal", "-i", str(intermediate), "-a", str(output_4), "-g", "4", "-q", "-o", str(temp_output)])
+			else:
+				#Metagenome mode for very short genomes.
+				subprocess.call(["prodigal", "-i", str(intermediate), "-p", "meta", "-a", str(output_11), "-q", "-o", str(temp_output)])				
+				subprocess.call(["prodigal", "-i", str(intermediate), "-p", "meta", "-a", str(output_4), "-g", "4", "-q", "-o", str(temp_output)])
+			'''
+			
+			subprocess.call(["prodigal", "-i", str(intermediate), "-a", str(output_11), "-q", "-o", str(temp_output)])				
+			subprocess.call(["prodigal", "-i", str(intermediate), "-a", str(output_4), "-g", "4", "-q", "-o", str(temp_output)])
+			
+			#We can get rid of the temp file immediately, we won't be using it
+			temp_output.unlink()
+			if genome_parser.is_gz:
+				#If the file was copied, delete. Otw. this would delete the input and we don't want that.
+				intermediate.unlink()
+
+			# Compare translation tables
+			length_4 = 0
+			length_11 = 0
+			with open(output_4, 'r') as table_4:
+				for line in table_4:
+					if line.startswith(">"):
+						continue
+					else:
+						length_4 += len(line.strip())
+
+			with open(output_11, 'r') as table_11:
+				for line in table_11:
+					if line.startswith(">"):
+						continue
+					else:
+						length_11 += len(line.strip())
+			
+			#Select the winning translation table and remove the other. Open the winner.
+			if (length_4 / length_11) >= 1.1:
+				output_11.unlink()
+				self.trans_table = "4"
+				chosen_protein = open(output_4, 'r')
+				table_11 = False
+			else:
+				output_4.unlink()
+				self.trans_table = "11"
+				chosen_protein = open(output_11, 'r')
+				table_11 = True
+				
+			destination = open(protein_output, "w")
+			
+			#Clean the winning output.
+			for line in chosen_protein:
+				if line.startswith(">"):
+					destination.write("{}".format(line))
+				else:
+					line = line.replace('*', '')
+					destination.write("{}".format(line))
+			
+			destination.close()
+			chosen_protein.close()
+			
+			# Remove the winning intermediate file, since we have the cleaned output
+			if table_11:
+				output_11.unlink()
+			else:
+				output_4.unlink()
+			
+			self.set_protein(str(protein_output))
+
+	#run hmmsearch on a protein	
+	def protein_to_hmm(self):
+		if self.protein is None:
+			print(self.name, "wasn't a declared as a protein! I can't make this into an HMM!")
+		else:
+		
+			folder = Path(self.output + "/hmms")
+						
+			hmm_output = folder / (self.basename + '.hmm')
+			temp_output = folder / (self.basename + '.temp')
+			
+			intermediate = folder / (self.basename + '_protein_intermediate.faa')
+			
+			current_protein = ""
+			current_seq = ""
+			
+			protein_parser = agnostic_reader(self.protein)
+			
+			#File was a gzip; decompress it to an intermediate file and then run prodigal; delete after
+			#Keeps track of \n chars in the protein sequences.
+			line_ct = 0
+			midpoint = open(intermediate, "w")
+			
+			for line in protein_parser:
+				if line.startswith(">"):
+					if len(current_seq) > 0:
+						if len(current_seq) < 100000:
+							midpoint.write(current_protein)
+							midpoint.write(current_seq)
+						else:
+							self.err_log += "Protein " + current_protein.strip().split()[0][1:] + " was observed to have >100K amino acids ( " + str(len(current_seq) - line_ct) + " AA found ). It was skipped. "
+							#print("Protein", current_protein.strip()[1:], "was observed to have >100K amino acids (", len(current_seq) - line_ct, "AA found ).", file = sys.stderr)
+							#print("HMMER cannot handle sequences that long, and the protein is almost certainly erroneous, anyway.", file = sys.stderr)
+							#print("The protein will be skipped, and FastAAI will continue without it.", file = sys.stderr)
+							
+					current_protein = line
+					current_seq = ""
+					line_ct = 0
+				else:
+					line_ct += 1
+					current_seq += line
+			
+			protein_parser.close()
+			
+			#Finally, last prot
+			if len(current_seq) > 0:
+				if len(current_seq) < 100000:
+					midpoint.write(current_protein)
+					midpoint.write(current_seq)
+				else:
+					self.err_log += "Protein " + current_protein.strip().split()[0][1:] + " was observed to have >100K amino acids ( " + str(len(current_seq) - line_ct) + " AA found ). It was skipped. "
+					#print("Protein", current_protein.strip()[1:], "was observed to have >100K amino acids (", len(current_seq) - line_ct, "AA found ).", file = sys.stderr)
+					#print("HMMER cannot handle sequences that long, and the protein is almost certainly erroneous, anyway.", file = sys.stderr)
+					#print("The protein will be skipped, and FastAAI will continue without it.", file = sys.stderr)
+			
+			midpoint.close()
+			
+			#Should locate the DBs regardless of path.
+			script_path = Path(__file__)
+			script_dir = script_path.parent
+			hmm_complete_model = script_dir / "00.Libraries/01.SCG_HMMs/Complete_SCG_DB.hmm"
+			
+			subprocess.call(["hmmsearch", "--tblout", str(hmm_output), "-o", str(temp_output), "--cut_tc", "--cpu", "1",
+							str(hmm_complete_model), str(intermediate)])
+							
+			temp_output.unlink()
+			intermediate.unlink()
+			
+			self.set_hmm(str(hmm_output))
+	
+	def prot_and_hmm_to_besthits(self):
+		prots = []
+		accs = []
+		scores = []
+		f = agnostic_reader(self.hmm)
+		for line in f:
+			if line.startswith("#"):
+				continue
+			else:
+				segs = line.strip().split()
+				prots.append(segs[0])
+				accs.append(segs[3])
+				scores.append(segs[8])
+			
+		f.close()
+		
+		hmm_file = np.transpose(np.array([prots, accs, scores]))
+		
+		#hmm_file = np.loadtxt(hmm_file_name, comments = '#', usecols = (0, 3, 8), dtype=(str))
+		#Sort the hmm file based on the score column in descending order.
+		hmm_file = hmm_file[hmm_file[:,2].astype(float).argsort()[::-1]]
+		
+		#Identify the first row where each gene name appears, after sorting by score; 
+		#in effect, return the highest scoring assignment per gene name
+		#Sort the indices of the result to match the score-sorted table instead of alphabetical order of gene names
+		hmm_file = hmm_file[np.sort(np.unique(hmm_file[:,0], return_index = True)[1])]
+		
+		#Filter the file again for the unique ACCESSION names, since we're only allowed one gene per accession, I guess?
+		#Don't sort the indices, we don't care about the scores anymore.
+		hmm_file = hmm_file[np.unique(hmm_file[:,1], return_index = True)[1]]
+		
+		self.best_hits = dict(zip(hmm_file[:,0], hmm_file[:,1]))
+		
+		self.best_hits_kmers = {}
+		current_seq = ""
+		current_prot = ""
+		is_besthit = False
+		
+		prot = agnostic_reader(self.protein)
+		
+		for line in prot:
+			
+			if line.startswith(">"):
+				if len(current_seq) > 0:
+					kmer_set = unique_kmers(current_seq, 4)
+					self.protein_kmer_count[current_prot] = kmer_set.shape[0]
+					self.protein_count += 1
+					self.best_hits_kmers[current_prot] = kmer_set
+				#Select the best hit accession for this protein and just record that. We do not care about the names of the proteins.
+				current_prot = line[1:].strip().split(" ")[0]
+				if current_prot in self.best_hits:
+					current_prot = self.best_hits[current_prot]
+					is_besthit = True
+				else:
+					is_besthit = False
+				current_seq = ""
+			else:
+				if is_besthit:
+					current_seq += line.strip()
+				
+		prot.close()
+	
+		#Final iter. doesn't happen otw.
+		if current_prot in self.best_hits:
+			kmer_set = unique_kmers(current_seq, 4)
+			#kmer_set = [kmer_index[k] for k in  kmer_set]
+			self.protein_kmer_count[current_prot] = kmer_set.shape[0]
+			self.protein_count += 1
+			self.best_hits_kmers[current_prot] = kmer_set
+			
+		self.status = "finished preprocessing"
+	
+	def preprocess(self):
+		#There's no advancement stage for protein and HMM
+		if self.status == 'genome':
+			start_time = curtime()
+			#report = True
+			if self.start_time is None:
+				self.start_time = start_time
+			
+			if self.initial_state == "protein+HMM":
+				self.initial_state = "genome"
+			
+			self.genome_to_protein()
+			
+			
+		if self.status == 'protein':
+			start_time = curtime()
+			#report = True
+			if self.start_time is None:
+				self.start_time = start_time
+				
+			if self.initial_state == "protein+HMM":
+				self.initial_state = "protein"
+			
+			self.protein_to_hmm()
+			
+		if self.status == 'protein and hmm':
+			start_time = curtime()
+			
+			if self.start_time is None:
+				self.start_time = start_time
+				
+			self.prot_and_hmm_to_besthits()
+			
+		#Add an end time if either genome -> protein -> HMM or protein -> HMM happened.
+		if self.start_time is not None:
+			end_time = curtime()
+			self.end_time = end_time
+		else:
+			#Start was protein+HMM. There was no runtime, and intitial state is p+hmm
+			#self.initial_state = "protein+HMM"
+			self.start_time = "N/A"
+			self.end_time = "N/A"
+			
+		#Protein not generated on this run.
+		if self.trans_table is None:
+			self.trans_table = "unknown"
+	
+	'''
+	Viral functions
+	'''
+	#No translation table comparison for viruses. Slightly reduced logic.
+	def viral_genome_to_protein(self):
+		if self.genome is None:
+			print(self.name, "wasn't a declared as a genome! I can't make this into a protein!")
+		else:
+			folder = Path(self.output + "/predicted_proteins")
+			intermediate_protein_output = folder / (self.basename + '.intermediate.faa')
+			final_protein_output = folder / (self.basename + '.faa')
+			temp_output = folder / (self.basename + '.temp')
+	
+			subprocess.call(["prodigal", "-i", str(self.genome), "-a", str(intermediate_protein_output), "-p", "meta", "-q", "-o", str(temp_output)])
+			
+				# Remove intermediate files
+			temp_output.unlink()
+			
+			chosen_protein = open(intermediate_protein_output, 'r')
+			destination = open(final_protein_output, "w")
+			
+			for line in chosen_protein:
+				if line.startswith(">"):
+					destination.write("{}".format(line))
+				else:
+					line = line.replace('*', '')
+					destination.write("{}".format(line))
+	
+			destination.close()
+			chosen_protein.close()
+			
+			intermediate_protein_output.unlink()
+			
+			self.protein = str(protein_output)
+			self.status = 'protein'
+	
+
+'''
+Preprocessing functions
+				
+Read directories, advance files to hmms as needed.				
+'''
+#Toy function for passing to a pool
+def do_advance(input_file_object):
+	input_file_object.preprocess()
+	return input_file_object
+
+def initialize_preproc(index):
+	global kmer_index
+	kmer_index = index
+	
+#Function which takes an input list
+def advance_inputs(genomes = None, proteins = None, hmms = None, genomes_file = None, proteins_file = None, hmms_file = None, output = "FastAAI", threads = 1, verbose = False, db_name = ""):
+	inputs = []
+	
+	hmm_broke = False
+	
+	if genomes_file is not None:
+		fh = agnostic_reader(genomes_file)
+		
+		for line in fh:
+			clean = line.strip()
+			if not os.path.exists(clean):
+				print("I can't find file", clean, "Are you sure this file exists and can be found from your current directory using the path you supplied in the input file?")
+			else:
+				current_file = input_file(clean, output, verbose)
+				current_file.set_genome(clean)
+				inputs.append(current_file)
+				del current_file
+			
+		fh.close()
+		
+	if proteins_file is not None:
+		fh = agnostic_reader(proteins_file)
+		
+		for line in fh:
+			#GOTOGOTO
+			print(line)
+		
+			clean = line.strip()
+			if not os.path.exists(clean):
+				print("I can't find file", clean, "Are you sure this file exists and can be found from your current directory using the path you supplied in the input file?")
+			else:
+				current_file = input_file(clean, output, verbose)
+				current_file.set_protein(clean)
+				inputs.append(current_file)
+				del current_file
+			
+		fh.close()
+		
+	if hmms_file is not None:	
+		fh = agnostic_reader(hmms_file)
+		
+		hmm_pairs = []
+		
+		for line in fh:
+			clean = line.strip()
+			if not os.path.exists(clean):
+				print("I can't find file", clean, "Are you sure this file exists and can be found from your current directory using the path you supplied in the input file?")
+			else:
+				hmm_pairs.append(clean)
+				
+		fh.close()
+		
+		if len(hmm_pairs) != len(inputs):
+			print("Protein and HMM file counts differ! There must be one HMM per protein, generated from its paired protein! These pairs must be in the same order in your input file!")
+			hmm_broke = True
+		else:
+			for h, i in zip(hmm_pairs, inputs):
+				i.set_hmm(h)
+		
+	if genomes is not None:
+		set = os.listdir(genomes)
+		#Sort is used to ensure lexicographic ordering.
+		set.sort()
+		set = [os.path.normpath(genomes + "/" + file) for file in set]
+		
+		for file in set:
+			if not os.path.exists(file):
+				print("I can't find", file, "Are you sure this file exists in the directory you supplied?")
+			else:
+				current_file = input_file(file, output, verbose)
+				current_file.set_genome(file)
+				inputs.append(current_file)
+				del current_file
+		
+	if proteins is not None:
+		set = os.listdir(proteins)
+		set.sort()
+		set = [os.path.normpath(proteins + "/" + file) for file in set]
+		
+		for file in set:
+			if not os.path.exists(file):
+				print("I can't find", file, "Are you sure this file exists in the directory you supplied?")
+			else:
+				current_file = input_file(file, output, verbose)
+				current_file.set_protein(file)
+				inputs.append(current_file)
+				del current_file
+			
+	if hmms is not None:
+		set = os.listdir(hmms)
+		set.sort()
+		set = [os.path.normpath(hmms + "/" + file) for file in set]
+		
+		hmm_pairs = []
+		
+		for file in set:
+			if not os.path.exists(file):
+				print("I can't find", file, "Are you sure this file exists in the directory you supplied?")
+			else:
+				hmm_pairs.append(file)
+				
+		if len(hmm_pairs) != len(inputs):
+			print("Protein and HMM file counts differ! There must be one HMM per protein, generated from its paired protein! These must be in the same alphabetical order in their respective directories!")
+			hmm_broke = True
+		else:
+			for h, i in zip(hmm_pairs, inputs):
+				i.set_hmm(h)
+	
+	if hmm_broke:
+		print("FastAAI can't proceed without matching HMM and protein pairs.")
+		inputs = None
+		return inputs
+		
+	total_counts = len(inputs)
+	count = 0
+	last_pct = 0
+	
+	if verbose:
+		print("")
+	#progress bar - possible dangerous use of the return to line start sequence.
+		try:
+			percentage = 0
+			sys.stdout.write("Completion".rjust(3)+ ' |'+('#'*int(percentage/2)).ljust(50)+'| ' + ('%.2f'%percentage).rjust(7)+'% (Genome ' + str(count) + " of " + str(total_counts) + ') at ' + curtime()+"\n")
+			sys.stdout.flush()
+		except:
+			#It's not really a big deal if the progress bar cannot be printed.
+			pass
+	
+	results = []
+	
+	kmer_index_ = create_kmer_index()
+	pool = multiprocessing.Pool(threads, initializer=initialize_preproc, initargs = (kmer_index_,))
+	
+	for res in pool.imap(do_advance, inputs):
+		results.append(res)
+		if verbose:
+		#progress bar - possible dangerous use of the return to line start sequence.
+			try:
+				count += 1
+				percentage = (count/total_counts)*100
+				if int(percentage/2) > last_pct or partition == total_partitions:
+					sys.stdout.write('\033[A')
+					sys.stdout.flush()
+					sys.stdout.write("Completion".rjust(3)+ ' |'+('#'*int(percentage/2)).ljust(50)+'| ' + ('%.2f'%percentage).rjust(7)+'% (Genome ' + str(count) + " of " + str(total_counts) + ') at ' + curtime()+"\n")
+					sys.stdout.flush()
+					
+				last_pct = int(percentage/2)
+			except:
+				#It's not really a big deal if the progress bar cannot be printed.
+				pass
+	
+	pool.close()
+	pool.join()
+	
+	inputs = results
+
+	log_time = curtime()
+	
+	if os.path.exists(os.path.normpath(output + "/logs/" + os.path.splitext(os.path.basename(db_name))[0] + "_preprocessing_log.txt")):
+		preproc_log = open(os.path.normpath(output + "/logs/" + os.path.splitext(os.path.basename(db_name))[0] + "_preprocessing_log.txt"), "a")
+	else:
+		preproc_log = open(os.path.normpath(output + "/logs/" + os.path.splitext(os.path.basename(db_name))[0] + "_preprocessing_log.txt"), "w")
+		print("log_date", "genome_name", "started_as_a", "start_time", "end_time", "protein_translation_table", "errors", sep = "\t", file = preproc_log)
+	for i in inputs:
+		print(log_time, i.basename, i.initial_state, i.start_time, i.end_time, i.trans_table, i.err_log, sep = "\t", file = preproc_log)
+	preproc_log.close()
+	
+	return inputs
+	
+'''
+Utility functions
+'''
+def prepare_directories(output, status, build_or_query):
+	preparation_successful = True
+	
+	if not os.path.exists(output):
+		try:
+			os.mkdir(output)
+		except:
+			print("")
+			print("FastAAI tried to make output directory: '"+ output + "' but failed.")
+			print("")
+			print("Troubleshooting:")
+			print("")
+			print("    (1) Do you have permission to create directories in the location you specified?")
+			print("    (2) Did you make sure that all directories other than", os.path.basename(output), "already exist?")
+			print("")
+			preparation_successful = False
+	
+	if preparation_successful:
+		try:
+			if status == 'genome':
+				if not os.path.exists(os.path.normpath(output + "/" + "predicted_proteins")):
+					os.mkdir(os.path.normpath(output + "/" + "predicted_proteins"))
+				if not os.path.exists(os.path.normpath(output + "/" + "hmms")):
+					os.mkdir(os.path.normpath(output + "/" + "hmms"))
+			
+			if status == 'protein':
+				if not os.path.exists(os.path.normpath(output + "/" + "hmms")):
+					os.mkdir(os.path.normpath(output + "/" + "hmms"))
+					
+			if not os.path.exists(os.path.normpath(output + "/" + "logs")):
+				os.mkdir(os.path.normpath(output + "/" + "logs"))
+			
+			if build_or_query == "build":
+				if not os.path.exists(os.path.normpath(output + "/" + "database")):
+					os.mkdir(os.path.normpath(output + "/" + "database"))
+			
+			if build_or_query == "query":		
+				if not os.path.exists(os.path.normpath(output + "/" + "results")):
+					os.mkdir(os.path.normpath(output + "/" + "results"))
+
+			
+		except:
+			print("FastAAI was able to create or find", output, "but couldn't make directories there.")
+			print("")
+			print("This shouldn't happen. Do you have permission to write to that directory?")
+			
+		
+	return preparation_successful	
+
+def check_out_input_files(genomes, proteins, hmms, gf, pf, hf):
+	#Check only one method of supply was used per file type
+	if (genomes is not None) and (gf is not None):
+		print("Supply genomes either by directory or by file, not both.")
+		return None
+	if (proteins is not None) and (pf is not None):
+		print("Supply proteins either by directory or by file, not both.")
+		return None
+	if (hmms is not None) and (hf is not None):
+		print("Supply HMMs either by directory or by file, not both.")
+		return None
+	
+	#check that not both proteins and genomes supplied in any combo.
+	if ((genomes is not None) and (pf is not None))\
+	or ((gf is not None) and (proteins is not None))\
+	or ((genomes is not None) and (proteins is not None))\
+	or ((gf is not None) and (pf is not None)):
+		print("Supply either genomes or proteins, not both. You can supply proteins and HMMs, but not genomes and proteins.")
+		return None
+		
+	#Check that if hmms are given, so are proteins
+	if (hmms is not None) or (hf is not None):
+		if (proteins is None) and (pf is None):
+			print("If you supply HMMs, you also have to supply the proteins from which they were generated.")
+			return None
+		
+	#Determine status
+	if (genomes is not None) or (gf is not None):
+		print("Starting from genomes")
+		start = 'genome'
+			
+	else:
+		if 	(hmms is not None) or (hf is not None):
+			print("Starting from proteins and HMMs")
+			start = 'protein and HMM'
+			
+		else:
+			print("Starting from proteins")
+			start = 'protein'
+			
+	return start
+
+
+#Build DB from genomes
+	
+def unique_kmers(seq, ksize):
+	n_kmers = len(seq) - ksize + 1
+	kmers = []
+	for i in range(n_kmers):
+		kmers.append(kmer_index[seq[i:i + ksize]])
+	#We care about the type because we're working with bytes later.
+	return np.unique(kmers).astype(np.int32)
+
+#Quickly creates a dict of all poss. tetramers in a fixed, alphabetical order.
+#This can be used to index kmers so that the indices are identical (and thus interchangable) on separate runs of this program.	
+def create_kmer_index():
+	valid_chars = ['A', 'C', 'D', 'E', 'F', 'G', 'H', 'I', 'K', 'L', 'M', 'N', 'P', 'Q', 'R', 'S', 'T', 'V', 'W', 'X', 'Y', '*']
+	#This meshgrid method will produce all unique tetramers from AAAA to **** in a consistent order.
+	#Rightmost char to leftmost, A to * in the same order as valid_chars
+	kmer_index_ = np.stack(np.meshgrid(valid_chars, valid_chars, valid_chars, valid_chars), -1).reshape(-1, 4)
+	#Unless someone is passing more than 2.1 billion genomes, int32 will be enough.
+	kmer_index_ = dict(zip([''.join(kmer_index_[i,]) for i in range(0, kmer_index_.shape[0])], np.arange(kmer_index_.shape[0], dtype = np.int32)))
+	
+	return kmer_index_
+
+def split_seq(seq, num_grps):
+	newseq = []
+	splitsize = 1.0/num_grps*len(seq)
+	for i in range(num_grps):
+		newseq.append(seq[int(round(i*splitsize)):int(round((i+1)*splitsize))])
+	return newseq
+	
+#gives the max and min index needed to split a list of (max_val) genomes into 
+def split_indicies(max_val, num_grps):
+	newseq = []
+	splitsize = 1.0/num_grps*max_val
+	for i in range(num_grps):
+		newseq.append(((round(i*splitsize)), round((i+1)*splitsize)))
+	return newseq
+	
+def list_to_index_dict(list):
+	result = {}
+	counter = 0
+	for item in list:
+		result[item] = counter
+		counter += 1
+	return result
+
+def generate_accessions_index():
+	list_of_poss_accs = list_to_index_dict(['PF01780.19', 'PF03948.14', 'PF17144.4', 'PF00830.19', 'PF00347.23', 'PF16906.5', 'PF13393.6',
+	'PF02565.15', 'PF01991.18', 'PF01984.20', 'PF00861.22', 'PF13656.6', 'PF00368.18', 'PF01142.18', 'PF00312.22', 'PF02367.17',
+	'PF01951.16', 'PF00749.21', 'PF01655.18', 'PF00318.20', 'PF01813.17', 'PF01649.18', 'PF01025.19', 'PF00380.19', 'PF01282.19',
+	'PF01864.17', 'PF01783.23', 'PF01808.18', 'PF01982.16', 'PF01715.17', 'PF00213.18', 'PF00119.20', 'PF00573.22', 'PF01981.16',
+	'PF00281.19', 'PF00584.20', 'PF00825.18', 'PF00406.22', 'PF00177.21', 'PF01192.22', 'PF05833.11', 'PF02699.15', 'PF01016.19',
+	'PF01765.19', 'PF00453.18', 'PF01193.24', 'PF05221.17', 'PF00231.19', 'PF00416.22', 'PF02033.18', 'PF01668.18', 'PF00886.19',
+	'PF00252.18', 'PF00572.18', 'PF00366.20', 'PF04104.14', 'PF04919.12', 'PF01912.18', 'PF00276.20', 'PF00203.21', 'PF00889.19',
+	'PF02996.17', 'PF00121.18', 'PF01990.17', 'PF00344.20', 'PF00297.22', 'PF01196.19', 'PF01194.17', 'PF01725.16', 'PF00750.19',
+	'PF00338.22', 'PF00238.19', 'PF01200.18', 'PF00162.19', 'PF00181.23', 'PF01866.17', 'PF00709.21', 'PF02006.16', 'PF00164.25',
+	'PF00237.19', 'PF01139.17', 'PF01351.18', 'PF04010.13', 'PF06093.13', 'PF00828.19', 'PF02410.15', 'PF01176.19', 'PF02130.17',
+	'PF01948.18', 'PF01195.19', 'PF01746.21', 'PF01667.17', 'PF03874.16', 'PF01090.19', 'PF01198.19', 'PF01250.17', 'PF17136.4',
+	'PF06026.14', 'PF03652.15', 'PF04019.12', 'PF01201.22', 'PF00832.20', 'PF01264.21', 'PF03840.14', 'PF00831.23', 'PF00189.20',
+	'PF02601.15', 'PF01496.19', 'PF00411.19', 'PF00334.19', 'PF00687.21', 'PF01157.18', 'PF01245.20', 'PF01994.16', 'PF01632.19',
+	'PF00827.17', 'PF01015.18', 'PF00829.21', 'PF00410.19', 'PF00833.18', 'PF00935.19', 'PF01992.16'])
+	
+	return list_of_poss_accs
+
+#Master function for building or adding to a DB with genomes.	
+def add_inputs(output_path, parent_path, existing_index, threads, verbose, prep_args):
+	
+	genomes, proteins, hmms, gf, pf, hf, db_name = prep_args[0], prep_args[1], prep_args[2], prep_args[3], prep_args[4], prep_args[5], prep_args[6]
+	
+	print("")
+	print("FastAAI is formatting your files to be saved to your database.")
+	
+	#Let's push this to the inputs section.
+	inputs = advance_inputs(genomes = genomes, proteins = proteins, hmms = hmms, genomes_file = gf, proteins_file = pf, hmms_file = hf, output = output_path, threads = threads, verbose = verbose, db_name = db_name)
+	
+	if inputs is None:
+		return False
+		
+	kmer_index = None
+	
+	#global genome_index
+	genome_index = {}
+	next_index = 0
+	
+	#Build upon the genome indexing of an existing DB
+	if existing_index is not None:
+		genome_index = existing_index
+		#zero indexing makes this the next number to add.
+		next_index = len(existing_index)
+		
+	final_db = fastaai_database(parent_path)
+	final_db.activate_connection()
+	final_db.initialize_parent_database()
+		
+	#This goes to the genome_index table
+	protein_counts_to_add = []
+	genome_acc_kmer_counts_to_add = []
+		
+	acc_index = generate_accessions_index()
+		
+	readied_kmers_by_acc = defaultdict(lambda: defaultdict(lambda: None))
+	
+	#unique_accessions = set()
+	for file in inputs:
+		
+		genome = file.basename
+		
+		#Collect all of the accessions actually found. Will usually be 122 for reasonably sized datasets.
+		#unique_accessions = unique_accessions.union(set(file.best_hits.values()))
+		#Avoid adding duplicate genomes
+		if genome not in genome_index:
+			protein_counts_to_add.append((genome, next_index, file.protein_count))
+			for prot in file.protein_kmer_count:
+				genome_acc_kmer_counts_to_add.append((next_index, acc_index[prot], file.protein_kmer_count[prot]))
+			genome_index[genome] = next_index
+			next_index += 1
+		
+		this_index = genome_index[genome]
+		for acc in file.best_hits_kmers:
+			readied_kmers_by_acc[acc][this_index] = file.best_hits_kmers[acc]
+		#Clean up space
+		file.best_hits_kmers = None
+
+	inputs = None
+	
+	#Default dicts can't be pickled.
+	readied_kmers_by_acc = dict(readied_kmers_by_acc)
+	
+	genomes_per_acc = {}
+	for acc in readied_kmers_by_acc:
+		readied_kmers_by_acc[acc] = dict(readied_kmers_by_acc[acc])
+		genomes_per_acc[acc] = list(readied_kmers_by_acc[acc].keys())
+		final_db.add_genomes_first(acc, readied_kmers_by_acc[acc])
+		readied_kmers_by_acc[acc] = None
+		
+	readied_kmers_by_acc = None
+		
+	add_genomes = "INSERT OR REPLACE INTO genome_index VALUES (?, ?, ?)"
+	add_proteins = "INSERT OR REPLACE INTO genome_acc_kmer_counts VALUES (?, ?, ?)"
+	
+	final_db.cursor.executemany(add_genomes, protein_counts_to_add)
+	final_db.cursor.executemany(add_proteins, genome_acc_kmer_counts_to_add)
+	final_db.connection.commit()
+	
+	final_db.cursor.execute("CREATE INDEX IF NOT EXISTS kmer_acc ON genome_acc_kmer_counts (genome, accession);")
+	final_db.connection.commit()
+	
+	protein_counts_to_add = None
+	genome_acc_kmer_counts_to_add = None
+			
+	unique_accessions = list(genomes_per_acc.keys())
+	child_args = []
+	for i in range(0, len(unique_accessions)):
+		accession = unique_accessions[i]
+		name = "accession_" + unique_accessions[i] + "_partition_" + str(i)
+		child_path = os.path.normpath(output_path+"/temp")
+		child_args.append([accession, name, child_path, parent_path, genomes_per_acc[accession], genome_index])
+			
+	print("")
+	print("Formatting data to add to database at", curtime())
+	
+	#Add partition, output, parent DB data.
+	if not os.path.exists(os.path.normpath(output_path+"/temp")):
+		try:
+			os.mkdir(os.path.normpath(output_path+"/temp"))
+		except:
+			print("Output directory failed to create! Cannot continue.")
+			return False
+	
+	if verbose:
+		print("")
+		count = 0
+		total_counts = len(child_args)
+		try:
+			log_time = curtime()
+			percentage = (count/total_counts)*100
+			sys.stdout.write("Completion".rjust(3)+ ' |'+('#'*int(percentage/2)).ljust(50)+'| ' + ('%.2f'%percentage).rjust(7)+'% ( ' + str(count) + " of " + str(total_counts) + ' ) at ' + curtime() + "\n")
+			sys.stdout.flush()
+		except:
+			#It's not really a big deal if the progress bar cannot be printed.
+			pass
+	
+	last_pct = 0
+	
+	quiverfull = []
+	
+	pool = multiprocessing.Pool(threads)
+	
+	for result in pool.imap_unordered(produce_children, child_args):
+		acc = result[0]
+		child = result[1]
+		
+		quiverfull.append([acc, child])
+		
+		if verbose:
+			count += 1
+			try:
+				percentage = (count/total_counts)*100
+				log_time = curtime()
+				sys.stdout.write('\033[A')
+				sys.stdout.flush()
+				sys.stdout.write("Completion".rjust(3)+ ' |'+('#'*int(percentage/2)).ljust(50)+'| ' + ('%.2f'%percentage).rjust(7)+'% ( ' + str(count) + " of " + str(total_counts) + ' done at '+ curtime() + " )\n")
+				sys.stdout.flush()
+			except:
+				#It's not really a big deal if the progress bar cannot be printed.
+				pass
+		
+	pool.close()
+	pool.join()
+	
+	print("")
+	print("Adding data to final database.")
+	
+	if verbose:
+		print("")
+		
+		count = 0
+		total_counts = len(child_args)
+		try:
+			percentage = (count/total_counts)*100
+			
+			("Completion".rjust(3)+ ' |'+('#'*int(percentage/2)).ljust(50)+'| ' + ('%.2f'%percentage).rjust(7)+'% ( ' + str(count) + " of " + str(total_counts) + ' done at '+ curtime() + " )\n")
+			sys.stdout.flush()		
+		except:
+			#It's not really a big deal if the progress bar cannot be printed.
+			pass
+	
+	last_pct = 0
+	
+	for result in quiverfull:
+		acc = result[0]
+		child = result[1]
+		final_db.add_child_to_parent(acc, child)
+
+		if verbose:
+			count += 1
+			try:
+				percentage = (count/total_counts)*100
+				log_time = curtime()
+				sys.stdout.write('\033[A')
+				sys.stdout.flush()
+				sys.stdout.write("Completion".rjust(3)+ ' |'+('#'*int(percentage/2)).ljust(50)+'| ' + ('%.2f'%percentage).rjust(7)+'% ( ' + str(count) + " of " + str(total_counts) + ' done at '+ curtime() + " )\n")
+				sys.stdout.flush()
+			except:
+				#It's not really a big deal if the progress bar cannot be printed.
+				pass
+	
+	
+	print("")
+	#print("Cleaning up...")
+	#final_db.connection.execute("VACUUM")
+	
+	final_db.close_connection()
+	
+	os.rmdir(os.path.normpath(output_path+"/temp"))
+	
+	return True
+
+#genome_index is global already
+def produce_children(args):
+	acc = args[0]
+	partition = args[1]
+	output_base = args[2]
+	parent_db = args[3]
+	genomes_in_this_acc = args[4]
+	genome_index = args[5]
+	
+	parental_database = fastaai_database(parent_db)
+	
+	sql_friendly_accession = acc.replace('.', '_')
+	
+	read_parent_sql = "SELECT * FROM " + sql_friendly_accession + "_genomes WHERE genome IN ({genomes})".format(genomes=','.join(['?']*len(genomes_in_this_acc)))
+	
+	parental_database.activate_connection()
+	
+	genomes_for_this_acc = dict(parental_database.cursor.execute(read_parent_sql, genomes_in_this_acc).fetchall())
+	
+	parental_database.close_connection()
+		
+	child_db = os.path.normpath(output_base + "/" + partition + ".db")
+	
+	this_child = child_database(child_db, parent_db)
+	
+	this_child.activate_child_connection()
+	#this_child.initialize_child_database()
+	this_child.activate_parent_connection()
+	
+	#Keys are genomes as indices, values are numpy arrays of kmers. This makes tuples.
+	#this_child.add_genomes_first(acc, zip(genomes_for_this_acc.keys(), genomes_for_this_acc.values()))
+	
+	#Here's where we add the genomes as such to the children, too.
+	readied_kmers = defaultdict(lambda: [])
+	for genome in genomes_for_this_acc:
+		for kmer in genomes_for_this_acc[genome]:
+			readied_kmers[kmer].append(genome)
+		#cleanup space
+		genomes_for_this_acc[genome] = None
+	
+	del genomes_for_this_acc
+	
+	readied_kmers = dict(readied_kmers)
+	for kmer in readied_kmers:
+		readied_kmers[kmer] = np.array(readied_kmers[kmer], dtype = np.int32)
+	
+	sql_friendly_accession = this_child.add_accession(acc, readied_kmers)
+		
+	this_child.close_parent_connection()
+	this_child.close_child_connection()
+	
+	del readied_kmers
+	
+	return [sql_friendly_accession, child_db]
+	
+#Build or add to a FastAAI DB
+def build_db_opts():
+	parser = argparse.ArgumentParser(formatter_class=argparse.RawTextHelpFormatter,
+			description='''
+	This FastAAI module allows you to create a FastAAI database from one or many genomes, proteins, or proteins and HMMs, or add these files to an existing one.
+	
+	Supply genomes OR proteins OR proteins AND HMMs as inputs.
+	
+	If you supply genomes, FastAAI will predict proteins from them, and HMMs will be created from those proteins
+	If you supply only proteins, FastAAI will create HMM files from them, searching against FastAAI's internal database
+	If you supply proteins AND HMMs, FastAAI will directly use them to build the database.\n
+	You cannot supply both genomes and proteins
+	''')
+
+	parser.add_argument('-g', '--genomes',  dest = 'genomes', default = None, help =  'A directory containing genomes in FASTA format.')
+	parser.add_argument('-p', '--proteins', dest = 'proteins', default = None, help = 'A directory containing protein amino acids in FASTA format.')
+	parser.add_argument('-m', '--hmms',     dest = 'hmms', default = None, help =     'A directory containing the results of an HMM search on a set of proteins.')
+	parser.add_argument('-d', '--database', dest = 'db_name', default = "FastAAI_database.sqlite.db", help =  'The name of the database you wish to create or add to. The database will be created if it doesn\'t already exist and placed in the output directory. FastAAI_database.sqlite.db by default.')
+	
+	parser.add_argument('-o', '--output',   dest = 'output', default = "FastAAI", help = 'The directory to place the database and any protein or HMM files FastAAI creates. By default, a directory named "FastAAI" will be created in the current working directory and results will be placed there.')
+						
+	parser.add_argument('--genome_file',    dest = 'gf', default = None, help =  'Alternative way to supply genomes. A file containing paths to your genome files, 1 per line.')
+	parser.add_argument('--protein_file',   dest = 'pf', default = None, help = 'Alternative way to supply proteins. A file containing paths to your protein files, 1 per line.')
+	parser.add_argument('--hmm_file',       dest = 'hf', default = None, help =     'Alternative way to supply HMMs. A file containing paths to your HMM files, 1 per line.')
+		
+	parser.add_argument('--threads',  dest = 'threads', type=int, default = 1, help = 'The number of processors to use. Default 1.')
+	parser.add_argument('--verbose',        dest = 'verbose', action='store_true', help = 'Print minor updates to console. Major updates are printed regardless.')
+		
+	args, unknown = parser.parse_known_args()
+	
+	return parser, args
+		
+def build_db(genomes, proteins, hmms, db_name, output, threads, gf, pf, hf, verbose):
+	
+	start = check_out_input_files(genomes, proteins, hmms, gf, pf, hf)
+	
+	#If something failed, we stop.
+	if start is None:
+		return False
+	
+	good_to_go = prepare_directories(output, start, "build")
+	
+	if not good_to_go:
+		return False
+	
+	#Check if the db contains path info. Incl. windows version.
+	if "/" not in db_name and "\\" not in db_name:
+		final_database = os.path.normpath(output + "/database/" + db_name)
+	else:
+		#If the person insists that the db has a path, let them.
+		final_database = db_name
+		
+	#We'll skip trying this if the file already exists.
+	existing_genome_IDs = None
+	try:
+		if os.path.exists(final_database):
+			parent = fastaai_database(final_database)
+			parent.activate_connection()
+			
+			existing_genome_IDs = {}
+			sql_command = "SELECT genome, gen_id FROM genome_index"
+			for result in parent.cursor.execute(sql_command).fetchall():
+				genome = result[0]
+				id = int(result[1])
+				existing_genome_IDs[genome] = id
+			
+			parent.close_connection()
+	except:
+		print("You specified an existing file to be a database, but it does not appear to be a FastAAI database.")
+		print("FastAAI will not be able to continue. Please give FastAAI a different database name and continue.")
+		print("Exiting.")
+		return False
+	
+	
+	prep_args = [genomes, proteins, hmms, gf, pf, hf, db_name]
+	
+	#inputs, output_path, parent_path, existing_index, threads
+	success = add_inputs(output, final_database, existing_genome_IDs, threads, verbose, prep_args)
+	
+	if success:
+		print("Database build complete!")
+		
+	return success
+
+
+#DB query functionality - unlimited version
+def do_query_vs_target_aai_only(query_name, target_name, threads, output, precision, verbose):
+	if not os.path.exists(os.path.normpath(output+"/temp")):
+		os.mkdir(os.path.normpath(output+"/temp"))
+		
+	if precision == "low":
+		jacc_precision = np.float16
+	if precision == "med":
+		jacc_precision = np.float32
+	if precision == "high":
+		jacc_precision = np.float64
+	
+	#Save the file paths.
+	query = fastaai_database(query_name)
+	target = fastaai_database(target_name)
+	
+	query.activate_connection()
+	query.just_accessions()
+	query_len = query.cursor.execute("SELECT Count(*) FROM genome_index").fetchall()[0][0]
+	#query.close_connection()
+	target.activate_connection()
+	target.just_accessions()
+	target_len = target.cursor.execute("SELECT Count(*) FROM genome_index").fetchall()[0][0]
+	#target.close_connection()
+	
+	print("FastAAI will search", query_len, "query genomes against", target_len, "target genomes.")
+	
+	print("")
+	print("FastAAI is preparing your AAI search... ", end = '', flush = True)
+	
+	accessions_in_common = list(set(query.accessions).intersection(target.accessions))
+	
+	query.accessions = None
+	target.accessions = None
+	
+	query.close_connection()
+	target.close_connection()
+	
+	load_args = [(query, target, acc) for acc in accessions_in_common]
+	
+	loads = []
+	ordered_accs = []
+	
+	pool = multiprocessing.Pool(threads)
+	
+	for result in pool.imap(load_getter, load_args):
+		load = result[0]
+		acc = result[1]
+		#Load will be None if the accession is in both query and target, but they still don't share even a single Kmer. Unlikely, but it happened once, so it WILL happen again.
+		if load is not None:
+			loads.append(load)
+			ordered_accs.append(acc)
+	
+	pool.close()
+	pool.join()
+	
+	loads = np.array(loads)
+	ordered_accs = np.array(ordered_accs)
+	
+	order = loads.argsort()[::-1]
+	
+	loads = loads[order]
+	ordered_accs = ordered_accs[order]
+	
+	load_balancer = {}
+	accs_per_load = {}
+	for i in range(0, threads):
+		load_balancer[i] = 0
+		accs_per_load[i] = []
+	
+	for i in range(0, loads.shape[0]):
+		index = list(load_balancer.values()).index(min(list(load_balancer.values())))
+		#print(index, load)
+		load_balancer[index] += loads[i]
+		accs_per_load[index].append(int(ordered_accs[i]))
+		
+	del loads
+	del ordered_accs
+		
+	print("done!")
+	if verbose:
+		print("FastAAI has balanced the workload of calculating AAI from your data.")
+		for index in accs_per_load:
+			print("Thread", index, "will handle", len(accs_per_load[index]), "accessions.")
+	print("FastAAI is beginning the calculation of AAI between your query and target genomes.")
+	
+	del load_balancer
+	
+	input_queue = multiprocessing.Queue()
+	output_queue = multiprocessing.Queue()
+	
+	for thread in accs_per_load:
+		input_queue.put(accs_per_load[thread])
+		
+	for i in range(0, threads):
+		input_queue.put('STOP')
+	
+	for i in range(0, threads):
+		multiprocessing.Process(target=accession_worker, args=(input_queue, output_queue, query, target, query_len, target_len, jacc_precision)).start()
+	
+	print("")
+	
+	results = np.zeros(shape = (query_len, target_len), dtype = jacc_precision)
+	
+	#Counter to keep the threads running until the whole process is done.
+	donezo = threads
+	while donezo > 0:
+		row = output_queue.get()
+		try:
+			results[row[0]] += row[1]
+		except:
+			donezo -= 1
+	
+	print("AAI calculations complete. Formatting results for writing.")
+	
+	#global glob_prec
+	#glob_prec = jacc_precision
+	
+	rdb_name = os.path.normpath(output+"/temp/aai_calc_db.db")
+	rdb = calculation_database(rdb_name, precision)
+	rdb.activate_connection()
+	rdb.initialize_database()
+	
+	#Get the data ready for passing to children...
+	
+	results = np.split(results, query_len, axis = 0)
+	
+	insertable = []
+	#iterate over results and turn them into tuples.
+	for i in range(0, query_len):
+		insertable.append((i, results[i].tobytes()))
+		results[i] = None
+	
+	rdb.cursor.executemany("INSERT INTO jaccards VALUES (?, ?)", (insertable))
+	rdb.connection.commit()
+	
+	rdb.close_connection()
+	
+	del insertable
+	del results
+	
+	#Now we split the query genomes into chunk and have threads process each chunk in parallel with its respective shared prot counts.
+	query_chunks = split_indicies(query_len, threads)
+	query_args = [([rdb_name], query_chunks[i], output, query, target, precision) for i in range(0, threads)]
+	
+	print("Results formatted. Writing results starting at", curtime())
+	
+	pool = multiprocessing.Pool(threads)
+	
+	pool.map(finish_jaccards, query_args)
+	
+	pool.close()
+	pool.join()
+	
+	os.remove(rdb_name)
+	
+	print("FastAAI complete! Results at:", os.path.normpath(output+"/results/"))
+	
+	return None
+	
+#Assess the number of comparisons that will have to be made to complete an accession so that balanced loads can be passed to threads
+def load_getter(args):
+	query, target, accession = args[0], args[1], args[2]
+	query.activate_connection()
+	target.activate_connection()
+
+	original_index = generate_accessions_index()
+	accession_inverter = {}
+	for acc in original_index:
+		sql_friendly_accession = acc.replace(".", "_")
+		accession_inverter[original_index[acc]] = sql_friendly_accession
+
+	sql_friendly_accession = accession_inverter[accession].replace('.', '_')
+	sql = "SELECT kmer FROM "+ sql_friendly_accession
+	query.cursor.row_factory = lambda cursor, row: row[0]
+	#query_kmers = set(query.cursor.execute(sql).fetchall()).intersection()
+	target.cursor.row_factory = lambda cursor, row: row[0]
+	#target_kmers = target.cursor.execute(sql).fetchall()
+	
+	shared_kmers = list(set(query.cursor.execute(sql).fetchall()).intersection(target.cursor.execute(sql).fetchall()))
+	query.cursor.row_factory = None
+	target.cursor.row_factory = None
+	
+	bytes_sql = "SELECT sum(length(genomes)) FROM " + sql_friendly_accession + " WHERE kmer IN ({kmers})".format(kmers=','.join(['?']*len(shared_kmers)))
+	
+	if len(shared_kmers) > 0:
+		tgt_res = target.cursor.execute(bytes_sql, shared_kmers).fetchone()[0]
+		query_res = query.cursor.execute(bytes_sql, shared_kmers).fetchone()[0]
+		#This if *should* always happen, if it gets checked.
+		if tgt_res is not None and query_res is not None:
+			load = int(tgt_res/(4096) * query_res/(4096))
+		else:
+			load = None
+	else:
+		load = None
+	
+	query.close_connection()
+	target.close_connection()
+		
+	return [load, accession]
+	
+def accession_worker(in_queue, out_queue, query, target, qlen, tlen, prec):
+	original_index = generate_accessions_index()
+	accession_inverter = {}
+	for acc in original_index:
+		sql_friendly_accession = acc.replace(".", "_")
+		accession_inverter[original_index[acc]] = sql_friendly_accession
+
+	query.activate_connection()
+	target.activate_connection()
+	query.load_genome_index()
+	target.load_genome_index()
+	
+	for my_accessions in iter(in_queue.get, 'STOP'):
+	
+		#print(my_accessions)
+		
+		target.load_accessions(permitted_accessions = my_accessions)
+		query.load_accessions(permitted_accessions = my_accessions)
+		
+		query_data = {}
+		target_data = {}
+		
+		for acc in my_accessions:
+
+			sql_friendly_accession = accession_inverter[acc].replace('.', '_')
+			
+			query_data[acc] = dict(query.cursor.execute("SELECT * FROM "+sql_friendly_accession+"_genomes").fetchall())
+			
+			query.cursor.row_factory = lambda cursor, row: row[0]
+			selected_kmers = list(query.cursor.execute("SELECT kmer FROM "+sql_friendly_accession).fetchall())
+			query.cursor.row_factory = None
+			
+			target_sql = "SELECT * FROM " + sql_friendly_accession + " WHERE kmer in ({kmers})".format(kmers=','.join(['?']*len(selected_kmers)))
+			target_data[acc] = dict(target.cursor.execute(target_sql, selected_kmers).fetchall())
+			
+		target_kmer_cts_by_acc = {}
+		for acc in my_accessions:
+			target_kmer_cts_by_acc[acc] = np.zeros(tlen, dtype = np.int16)
+			
+		for genome in target.gak:
+			for acc in target.gak[genome]:
+				target_kmer_cts_by_acc[acc][genome] = target.gak[genome][acc]
+					
+		#No longer needed.
+		target.gak = None
+		#We want each thread to report every single genome
+		for genome in query.gak:
+			#count += 1
+			#print("Thread", my_thread, "genome", count, "of", total)
+			these_jaccards = np.zeros(tlen, dtype = np.float64)
+			for acc in query.gak[genome]:
+				these_intersections = np.zeros(tlen, dtype = np.int16)
+				query_kmers = query_data[acc][genome]
+				query_kmer_ct = query_kmers.shape
+				for kmer in query_kmers:
+					if kmer in target_data[acc]:
+						these_intersections[target_data[acc][kmer]] += 1
+						
+				these_jaccards += np.divide(these_intersections, np.subtract(np.add(query_kmer_ct, target_kmer_cts_by_acc[acc]), these_intersections))
+				
+			out_queue.put([genome, these_jaccards])
+			
+	target.close_connection()
+	query.close_connection()
+	out_queue.put("Based")
+
+	return None
+	
+def finish_jaccards(args):
+	partial_dbs, my_query_genomes, output, query, target, prec = args[0], args[1], args[2], args[3] ,args[4], args[5]
+	#Load protein counts
+	#for each genome, query each partial and sum matching genomes, then divide by shared counts.
+	
+	query.activate_connection()
+	target.activate_connection()
+	query.load_genome_index()
+	target.load_genome_index()
+	
+	selected_query_genomes = range(my_query_genomes[0], my_query_genomes[1])
+
+	offset = my_query_genomes[0]
+	
+	target_len = len(target.genome_index)
+	query_len = my_query_genomes[1] - my_query_genomes[0]
+	
+	#get shared protein counts
+	query.load_accessions(permitted_genomes = selected_query_genomes)
+	
+	max_acc = 122
+	
+	query_set = np.zeros(shape = (query_len, max_acc), dtype = np.int16)
+	
+	for g in query.gak:
+		query_set[(g-offset), list(query.gak[g])] += 1
+	
+	target_set = np.zeros(shape = (max_acc, len(target.genome_index)), dtype = np.int16)
+	
+	target.load_accessions()
+	
+	target_protein_counts = np.zeros(target_len, dtype = np.int16)
+	for t in target.gak:
+		target_set[list(target.gak[t]), t] += 1
+		target_protein_counts[t] = len(target.gak[t])
+		
+	#This will be used to divide the jaccs and such. If disk, then disk, tho...
+	shared_prot_counts_by_genome = np.dot(query_set, target_set)
+	
+	del query_set
+	del target_set
+	
+	target.gak = None
+	
+	query.close_connection()
+	target.close_connection()
+	
+	activated_DBs = []
+	idx = 0
+	for db in partial_dbs:
+		activated_DBs.append(calculation_database(db, prec))
+		activated_DBs[idx].activate_connection()
+		idx += 1
+
+	
+	for genome in selected_query_genomes:
+		sql = "SELECT jaccards FROM jaccards WHERE genome="+str(genome)
+		total_jaccs = np.zeros(target_len, dtype = np.float64)
+		shared_acc_counts = shared_prot_counts_by_genome[genome - offset]
+		for db in activated_DBs:
+			result = db.cursor.execute(sql).fetchone()[0]
+			total_jaccs += result
+			
+		total_jaccs = np.divide(total_jaccs, shared_acc_counts)
+	
+		aai_est = numpy_kaai_to_aai(total_jaccs)
+		
+		no_hit = np.where(shared_acc_counts == 0)
+		#Actual hits is already stored in shared_acc_counts
+		possible_hits = np.minimum(len(query.gak[genome]), target_protein_counts).astype(str)
+		
+		total_jaccs = np.round(total_jaccs, 4).astype(str)
+		
+		shared_acc_counts = shared_acc_counts.astype(str)
+			
+		total_jaccs[no_hit] = "N/A"
+		aai_est[no_hit] = "N/A"
+		shared_acc_counts[no_hit] = "N/A"
+		possible_hits[no_hit] = "N/A"
+		
+		name = query.reverse_genome_index[genome]
+		
+		output_file = output +"/results/"+name+"_results.txt"
+		fh = open(output_file, "w")
+
+		for tgt in range(0, target_len):
+			target_name = target.reverse_genome_index[tgt]
+			if target_name == name:
+				fh.write(name+"\t"+target_name+"\t"+"100.0"+"\t"+"0.0"+"\t"+shared_acc_counts[tgt]+"\t"+possible_hits[tgt]+"\t"+"100.0"+"\n")
+			else:
+				fh.write(name+"\t"+target_name+"\t"+total_jaccs[tgt]+"\t"+"N/A"+"\t"+shared_acc_counts[tgt]+"\t"+possible_hits[tgt]+"\t"+aai_est[tgt]+"\n")
+		
+		fh.close()
+		
+		#Write partial to file, here.
+	
+	for db in activated_DBs:
+		db.close_connection()
+	
+	return None
+	
+	
+#Here's the DB SQL querying functionality/limited version.
+def do_query_vs_target_sql(query, target, threads, output, verbose, do_stdev):
+	#Save the file paths.
+	query_name, target_name = query, target
+	
+	query = fastaai_database(query_name)
+	query.activate_connection()
+	query.load_genome_index()
+	query.just_accessions()
+	
+	converter = generate_accessions_index()
+	acc_sql = "SELECT name FROM sqlite_master WHERE type='table'"
+	tables = [item[0] for item in query.cursor.execute(acc_sql).fetchall()]
+	cleaned_tables = []
+	for table in tables:
+		if table.endswith("_genomes"):
+			acc_name = table.split("_genomes")[0]
+			acc_name = acc_name.replace("_", ".")
+			index = acc_name
+			cleaned_tables.append((table, index))
+
+	del tables
+			
+	#Go through tables and load data.
+	query_acc_kmers = defaultdict(dict)
+	
+	sys.stdout.write("\n")
+	sys.stdout.write("Loading query data at " + curtime() + " ...\n")
+	sys.stdout.flush()
+	
+	for tab_idx in cleaned_tables:
+		table = tab_idx[0]
+		accession = tab_idx[1]
+		for result in query.cursor.execute("SELECT * FROM " + table).fetchall():
+			query_acc_kmers[result[0]][accession] = result[1]
+		
+	query.close_connection()
+		
+		
+	sys.stdout.write("\n")
+	sys.stdout.write("Loading target data at " + curtime() + " ...\n")
+	sys.stdout.flush()
+		
+	target = fastaai_database(target_name)
+	target.activate_connection()
+	target.load_genome_index()
+	target.load_accessions()
+	target.close_connection()
+		
+	query_args = []
+	for genome in query_acc_kmers:
+		query_args.append((target, query.reverse_genome_index[genome], query_acc_kmers[genome], os.path.normpath(output+"/results")))
+	
+	detected_query_accs = query.accessions
+	query_length = len(query.genome_index)
+	
+	#Cleanup
+	del query
+	del query_acc_kmers
+	
+	#global target_kmer_cts
+	target_kmer_cts = {}
+	
+	target_len = len(target.gak)
+	
+	for accession in np.intersect1d(detected_query_accs, target.accessions):
+		target_kmer_cts[accession] = np.zeros(target_len, dtype = np.int16)
+		for g in target.gak:
+			if accession in target.gak[g]:
+				target_kmer_cts[accession][g] = target.gak[g][accession]
+	
+	#global target_protein_counts
+	target_protein_counts = np.zeros(target_len, dtype = np.int16)
+	for g in target.gak:
+		target_protein_counts[g] = len(target.gak[g])
+	
+	target_length = len(target.gak)
+	
+	target.gak = None
+	
+	#Should just load the stuff then straightforward sql
+	sys.stdout.write("\n")
+	sys.stdout.write("FastAAI will search "+ str(query_length) + " query genomes against " + str(target_length) + " target genomes.\n")
+	sys.stdout.write("\n")
+	
+	count = 0
+	total = len(query_args)
+	
+	sys.stdout.write("Beginning AAI calculation at " + curtime())
+	
+	if verbose:
+		print("")
+	#progress bar - possible dangerous use of the return to line start sequence.
+		try:
+			percentage = 0
+			sys.stdout.write("Completion".rjust(3)+ ' |'+('#'*int(percentage/2)).ljust(50)+'| ' + ('%.2f'%percentage).rjust(7)+'% (Query genome ' + str(count) + " of " + str(total) + ' done at '+curtime()+')\n')
+			sys.stdout.flush()
+			last_pct = 0
+		except:
+			#It's not really a big deal if the progress bar cannot be printed.
+			pass
+
+	pool = multiprocessing.Pool(threads, initializer = sql_query_thread_starter, initargs = (target_kmer_cts, target_protein_counts,))
+
+	#Process as we go.
+	if do_stdev:
+		for file in pool.imap(do_sql_query, query_args):
+			if verbose:
+			#progress bar - possible dangerous use of the return to line start sequence.
+				try:
+					count += 1
+					percentage = (count/total)*100
+					if int(percentage/2) > last_pct or count == total:
+						sys.stdout.write('\033[A')
+						sys.stdout.write("Completion".rjust(3)+ ' |'+('#'*int(percentage/2)).ljust(50)+'| ' + ('%.2f'%percentage).rjust(7)+'% (Query genome ' + str(count) + " of " + str(total) + ' done at '+curtime()+')\n')
+						sys.stdout.flush()
+					last_pct = int(percentage/2)
+				except:
+					#It's not really a big deal if the progress bar cannot be printed.
+					pass
+		
+		pool.close()
+		pool.join()
+	else:
+	
+		for file in pool.imap(do_sql_query_no_SD, query_args):
+			
+			if verbose:
+				#progress bar - possible dangerous use of the return to line start sequence.
+				try:
+					count += 1
+					percentage = (count/total)*100
+					if int(percentage/2) > last_pct or count == total:
+						sys.stdout.write('\033[A')
+						sys.stdout.write("Completion".rjust(3)+ ' |'+('#'*int(percentage/2)).ljust(50)+'| ' + ('%.2f'%percentage).rjust(7)+'% (Query genome ' + str(count) + " of " + str(total) + ' done at '+curtime()+')\n')
+						sys.stdout.flush()
+					last_pct = int(percentage/2)
+				except:
+					#It's not really a big deal if the progress bar cannot be printed.
+					pass
+		
+		pool.close()
+		pool.join()
+	
+	print("AAI calculation complete! Results at:", os.path.normpath(output+"/results"))
+
+	return None
+	
+#This can also take the genomes-first formatted prots in the DB and search them memory-efficiently, if not time efficiently.
+def do_sql_query(args):
+	kmer_index = create_kmer_index()
+	accession_index = generate_accessions_index()
+	#database, file.basename, file.best_hits_kmers, os.path.normpath(output+"/temp")
+	database, name, acc_kmers, temp_out = args[0],args[1],args[2],args[3]
+	
+	database.activate_connection()
+	
+	res_ct = 0
+	target_len = len(database.genome_index)
+	
+	results = np.zeros(shape = (len(acc_kmers), target_len), dtype = np.float64)
+	row = 0
+	
+	shared_acc_counts = np.zeros(target_len, dtype = np.int16)
+	
+	for accession in acc_kmers:
+		acc_index = accession_index[accession]
+		sql_friendly_accession = accession.replace(".", "_")
+		if acc_index in database.accessions:
+			#The accession was found for this target genome, for each tgt genome.
+			shared_acc_counts[np.nonzero(target_kmer_cts[acc_index])] += 1
+			these_kmers = [int(kmer) for kmer in acc_kmers[accession]]
+			these_intersections = np.zeros(target_len, dtype = np.int16)
+			sql_query = "SELECT genomes FROM " + sql_friendly_accession + " WHERE kmer in ({kmers})".format(kmers=','.join(['?']*len(these_kmers)))
+			for result in database.cursor.execute(sql_query, these_kmers):
+				these_intersections[result] += 1
+		
+			results[row] = np.divide(these_intersections, np.subtract(np.add(acc_kmers[accession].shape[0], target_kmer_cts[acc_index]), these_intersections))
+		
+		row += 1
+	
+	database.close_connection()
+	
+	#These are the jacc averages
+	jaccard_averages = np.divide(np.sum(results, axis = 0), shared_acc_counts)
+	
+	#Get the differences from the mean per hit
+	results = results - jaccard_averages
+	#Square them
+	results = np.square(results)
+	#Sum squares and divide by shared acc. count, the sqrt to get SD.
+	jaccard_SDs = np.sqrt(np.divide(np.sum(results, axis = 0), shared_acc_counts))
+
+	aai_est = numpy_kaai_to_aai(jaccard_averages)
+	
+	no_hit = np.where(shared_acc_counts == 0)
+	#Actual hits is already stored in shared_acc_counts
+	possible_hits = np.minimum(len(acc_kmers), target_protein_counts).astype(str)
+	
+	
+	jaccard_averages = np.round(jaccard_averages, 4).astype(str)
+	jaccard_SDs = np.round(jaccard_SDs, 4).astype(str)
+	
+	shared_acc_counts = shared_acc_counts.astype(str)
+		
+	jaccard_averages[no_hit] = "N/A"
+	aai_est[no_hit] = "N/A"
+	jaccard_SDs[no_hit] = "N/A"
+	shared_acc_counts[no_hit] = "N/A"
+	possible_hits[no_hit] = "N/A"
+	
+	output_file = temp_out +"/"+name+"_results.txt"
+	fh = open(output_file, "w")
+
+	for target in range(0, target_len):
+		target_name = database.reverse_genome_index[target]
+		if target_name == name:
+			fh.write(name+"\t"+target_name+"\t"+"100.0"+"\t"+"0.0"+"\t"+shared_acc_counts[target]+"\t"+possible_hits[target]+"\t"+"100.0"+"\n")
+		else:
+			fh.write(name+"\t"+target_name+"\t"+jaccard_averages[target]+"\t"+jaccard_SDs[target]+"\t"+shared_acc_counts[target]+"\t"+possible_hits[target]+"\t"+aai_est[target]+"\n")
+	
+	fh.close()
+	
+	return output_file
+
+#This can also take the genomes-first formatted prots in the DB and search them memory-efficiently, if not time efficiently.
+def do_sql_query_no_SD(args):
+	kmer_index = create_kmer_index()
+	accession_index = generate_accessions_index()
+	#database, file.basename, file.best_hits_kmers, os.path.normpath(output+"/temp")
+	database, name, acc_kmers, temp_out = args[0],args[1],args[2],args[3]
+	
+	database.activate_connection()
+	
+	res_ct = 0
+	target_len = len(database.genome_index)
+	
+	results = np.zeros(shape = target_len, dtype = np.float64)
+	#row = 0
+	
+	shared_acc_counts = np.zeros(target_len, dtype = np.int16)
+	
+	for accession in acc_kmers:
+		acc_index = accession_index[accession]
+		sql_friendly_accession = accession.replace(".", "_")
+		if acc_index in database.accessions:
+			#The accession was found for this target genome, for each tgt genome.
+			shared_acc_counts[np.nonzero(target_kmer_cts[acc_index])] += 1
+			these_kmers = [int(kmer) for kmer in acc_kmers[accession]]
+			these_intersections = np.zeros(target_len, dtype = np.int16)
+			sql_query = "SELECT genomes FROM " + sql_friendly_accession + " WHERE kmer in ({kmers})".format(kmers=','.join(['?']*len(these_kmers)))
+			for result in database.cursor.execute(sql_query, these_kmers):
+				these_intersections[result] += 1
+			
+			results += np.divide(these_intersections, np.subtract(np.add(acc_kmers[accession].shape[0], target_kmer_cts[acc_index]), these_intersections))
+	
+	database.close_connection()
+	
+	#These are the jacc averages
+	jaccard_averages = np.divide(results, shared_acc_counts)
+	del results
+
+	aai_est = numpy_kaai_to_aai(jaccard_averages)
+	
+	no_hit = np.where(shared_acc_counts == 0)
+	
+	possible_hits = np.minimum(len(acc_kmers), target_protein_counts).astype(str)
+	
+	jaccard_averages = np.round(jaccard_averages, 4).astype(str)
+	
+	shared_acc_counts = shared_acc_counts.astype(str)
+		
+	jaccard_averages[no_hit] = "N/A"
+	aai_est[no_hit] = "N/A"
+	shared_acc_counts[no_hit] = "N/A"
+	possible_hits[no_hit] = "N/A"
+	
+	output_file = temp_out +"/"+name+"_results.txt"
+	fh = open(output_file, "w")
+
+	for target in range(0, target_len):
+		target_name = database.reverse_genome_index[target]
+		if target_name == name:
+			fh.write(name+"\t"+target_name+"\t"+"100.0"+"\t"+"0.0"+"\t"+shared_acc_counts[target]+"\t"+possible_hits[target]+"\t"+"100.0"+"\n")
+		else:
+			fh.write(name+"\t"+target_name+"\t"+jaccard_averages[target]+"\t"+"N/A"+"\t"+shared_acc_counts[target]+"\t"+possible_hits[target]+"\t"+aai_est[target]+"\n")
+	
+	fh.close()
+	
+	return output_file
+
+def numpy_kaai_to_aai(kaai_array):
+	#aai_hat = (-0.3087057 + 1.810741 * (np.exp(-(-0.2607023 * np.log(kaai))**(1/3.435))))*100
+	
+	#Protect the original jaccard averages memory item
+	aai_hat_array = kaai_array.copy()
+	
+	non_zero = np.where(aai_hat_array > 0)
+	is_zero  = np.where(aai_hat_array <= 0)
+	
+	#I broke this down into its original components
+	#Avoid zeroes in log - still actually works, but it produces warnings I don't want to see.
+	aai_hat_array[non_zero] = np.log(aai_hat_array[non_zero])
+	
+	aai_hat_array = np.multiply(np.subtract(np.multiply(np.exp(np.negative(np.power(np.multiply(aai_hat_array, -0.2607023), (1/3.435)))), 1.810741), 0.3087057), 100)
+	'''
+	Same as the above, broken down into easier-to-follow steps.
+	aai_hat_array = np.multiply(aai_hat_array, -0.2607023)
+	aai_hat_array = np.power(aai_hat_array, (1/3.435))
+	aai_hat_array = np.negative(aai_hat_array)
+	aai_hat_array = np.exp(aai_hat_array)
+	aai_hat_array = np.multiply(aai_hat_array, 1.810741)
+	aai_hat_array = np.subtract(aai_hat_array, 0.3087057)
+	aai_hat_array = np.multiply(aai_hat_array, 100)
+	'''
+	
+	#<30 and >90 values
+	smol = np.where(aai_hat_array < 30)
+	big  = np.where(aai_hat_array > 90)
+	
+	aai_hat_array = np.round(aai_hat_array, 2)
+	
+	#Convert to final printables
+	aai_hat_array = aai_hat_array.astype(str)
+	aai_hat_array[smol] = "<30%"
+	aai_hat_array[big]  = ">90%"
+	#The math of the above ends up with zero values being big, so we fix those.
+	aai_hat_array[is_zero] = "<30%"
+	
+	return aai_hat_array
+	
+def curtime():
+	time_format = "%d/%m/%Y %H:%M:%S"
+	timer = datetime.datetime.now()
+	time = timer.strftime(time_format)
+	return time
+
+#Manages the query process.
+def db_query_opts():
+	parser = argparse.ArgumentParser(formatter_class=argparse.RawTextHelpFormatter,
+			description='''
+	This FastAAI module takes two FastAAI databases and searches all of the genomes in the QUERY against all of the genomes in the TARGET
+	
+	If you have many genomes (more than 1000), it will be faster to create the query database using FastAAI build_db, 
+	then search it against an existing target using this module than it is to do the same thing with an SQL query.
+	
+	If you give the same database as query and target, a special all vs. all search of the genomes in the database will be done.
+	''')
+	parser.add_argument('-q', '--query',  dest = 'query',  default = None, help =  'Path to the query database. The genomes FROM the query will be searched against the genomes in the target database')
+	parser.add_argument('-t', '--target', dest = 'target', default = None, help =  'Path to the target database.')
+				
+	parser.add_argument('-o', '--output', dest = 'output', default = "FastAAI", help = 'The directory where FastAAI will place the result of this query. By default, a directory named "FastAAI" will be created in the current working directory and results will be placed there.')
+
+	parser.add_argument('--threads',      dest = 'threads', type=int, default = 1, help = 'The number of processors to use. Default 1.')
+	parser.add_argument('--verbose',      dest = 'verbose', action='store_true', help = 'Print minor updates to console. Major updates are printed regardless.')
+
+	parser.add_argument('--do_stdev',   dest = "do_stdev", action='store_true',                 help = 'Off by default. Calculate std. deviations on Jaccard indicies. Increases memory usage and runtime slightly. Does NOT change estimated AAI values at all.')
+	parser.add_argument('--unlimited_resources', dest = "large_mem",  action = 'store_true', help = 'Off by default. Use a faster algorithm that consumes more RAM. FastAAI cannot calculate std. deviations with this algorithm, so they will automatically be skipped.')
+	parser.add_argument('--mem',        dest = "precision", default = "med",                 help = 'One of low/med/high. Medium by default. Save RAM in return for slightly rounded AAI estimates. Only affects FastAAI if you are also using the "--unlimited_resources" flag.')
+
+	args, unknown = parser.parse_known_args()
+	
+	return parser, args
+	
+#Control the query process for any DB-first query.
+def db_query(query, target, verbose, output, threads, do_stdev, precision, memory_efficient):
+	print("")
+	
+	#Sanity checks.
+	if not os.path.exists(target):
+		print("Target database not found. Exiting FastAAI")
+		sys.exit()
+	
+	if not os.path.exists(query):
+		print("Query database not found. Exiting FastAAI")
+		sys.exit()
+		
+	#status = "exists"
+	query_ok = assess_db(query)
+	target_ok = assess_db(target)
+	
+	if query_ok != "exists":
+		print("Query database improperly formatted. Exiting FastAAI")
+		sys.exit()
+		
+	if target_ok != "exists":
+		print("Query database improperly formatted. Exiting FastAAI")
+		sys.exit()
+	
+	#Check if the database is querying against itself.
+	if target is None or query is None:
+		print("I require both a query and a target database. FastAAI exiting.")
+		sys.exit()
+	
+	if query == target:
+		print("Performing an all vs. all query on", query)
+		#all_vs_all = True
+	else:
+		print("Querying", query, "against", target)
+		#all_vs_all = False
+	
+	#Ready the output directories as needed.	
+	#The databases are already created, the only state they can be in in P+H
+	good_to_go = prepare_directories(output, "protein and HMM", "query")
+	if not good_to_go:
+		print("Exiting FastAAI")
+		sys.exit()
+	
+	if precision not in ["high", "med", "low"]:
+		print("Selected memory usage setting not found. Defaulting to med. Select one with --mem high/med/low.")
+		precision = 'med'
+	
+	#Default
+	if (not memory_efficient) or do_stdev:
+		do_query_vs_target_sql(query, target, threads, output, verbose, do_stdev)
+	#Not default.
+	else:
+		do_query_vs_target_aai_only(query, target, threads, output, precision, verbose)
+	
+	print("")
+	
+
+#Perform a minimal-memory query of a target database from input files. Lighter weight function for low memory
+def sql_query_opts():
+	parser = argparse.ArgumentParser(formatter_class=argparse.RawTextHelpFormatter,
+			description='''
+	This FastAAI module takes one or many genomes, proteins, or proteins and HMMs as a QUERY and searches them against an existing FastAAI database TARGET using SQL
+	If you only have a few genomes - or not enough RAM to hold the entire target database in memory - this is the probably the best option for you.
+	
+	If you provide FastAAI with genomes or only proteins (not proteins and HMMs), this FastAAI module will produce the required protein and HMM files as needed
+	and place them in the output directory, just like it does while building a database. 
+	
+	Once these inputs are ready to be queried against the database (each has both a protein and HMM file), they will be processed independently, 1 per thread at a time.
+	
+	Note: Protein and HMM files generated during this query can be supplied to build a FastAAI database from proteins and HMMs using the build_db module, without redoing preprocessing.
+	''')
+			
+	parser.add_argument('-g', '--genomes',  dest = 'genomes', default = None,  help =  'A directory containing genomes in FASTA format.')
+	parser.add_argument('-p', '--proteins', dest = 'proteins', default = None, help = 'A directory containing protein amino acids in FASTA format.')
+	parser.add_argument('-m', '--hmms',     dest = 'hmms', default = None,     help =     'A directory containing the results of an HMM search on a set of proteins.')
+	
+	parser.add_argument('--target',   dest = 'target', default = None, help =   'A path to the FastAAI database you wish to use as the target')
+	
+	parser.add_argument('-o', '--output',   dest = 'output', default = "FastAAI", help = 'The directory where FastAAI will place the result of this query and any protein or HMM files it has to generate. By default, a directory named "FastAAI" will be created in the current working directory and results will be placed there.')
+	
+	parser.add_argument('--genome_file',    dest = 'gf', default = None, help =  'Alternative way to supply genomes. A file containing paths to your genome files, 1 per line.')
+	parser.add_argument('--protein_file',   dest = 'pf', default = None, help = 'Alternative way to supply proteins. A file containing paths to your protein files, 1 per line.')
+	parser.add_argument('--hmm_file',       dest = 'hf', default = None, help =     'Alternative way to supply HMMs. A file containing paths to your HMM files, 1 per line.')
+	
+	parser.add_argument('--threads',  dest = 'threads', type=int, default = 1, help = 'The number of processors to use. Default 1.')
+	parser.add_argument('--verbose',        dest = 'verbose', action='store_true', help = 'Print minor updates to console. Major updates are printed regardless.')
+	
+	parser.add_argument('--do_stdev',   dest = "do_stdev", action='store_true',   help = 'Off by default. Calculate std. deviations on Jaccard indicies. Increases memory usage and runtime slightly. Does NOT change estimated AAI values at all.')
+	
+	args, unknown = parser.parse_known_args()
+	
+	return parser, args
+
+def sql_query_thread_starter(kmer_cts, protein_cts):
+	global target_kmer_cts 
+	global target_protein_counts
+	target_kmer_cts = kmer_cts
+	target_protein_counts = protein_cts
+
+	
+def sql_query(genomes, proteins, hmms, gf, pf, hf, db_name, output, threads, verbose, do_stdev):
+	
+	if not os.path.exists(db_name):
+		print("")
+		print("FastAAI can't find your database:", db_name)
+		print("Are you sure that the path you've given to the database is correct and that the database exists?")
+		print("FastAAI exiting.")
+		print("")
+		sys.exit()
+		
+	start = check_out_input_files(genomes, proteins, hmms, gf, pf, hf)
+	
+	#If something failed, we stop.
+	if start is None:
+		sys.exit()
+
+	
+	
+	good_to_go = prepare_directories(output, start, "query")
+	
+	if not good_to_go:
+		print("Exiting FastAAI")
+		sys.exit()
+	
+	#global kmer_index
+	#kmer_index = create_kmer_index()
+	
+	
+	print("")
+	print("Preparing inputs for querying...")
+	
+	prepared_files = advance_inputs(genomes = genomes, proteins = proteins, hmms = hmms, genomes_file = gf, proteins_file = pf, hmms_file = hf, output = output, threads = threads, verbose = verbose, db_name = db_name)
+	
+	if prepared_files is None:
+		return None
+	
+	query_accessions_detected = set()
+	for file in prepared_files:
+		query_accessions_detected = query_accessions_detected.union(file.best_hits.values())
+	
+	#We don't want to get more than we have to.
+	query_accessions_detected = list(query_accessions_detected)
+	
+	if prepared_files is None:
+		print("Exiting FastAAI")
+		sys.exit()
+	
+	if verbose:
+		print("")	
+	print("Gathering database information...")
+			
+	database = fastaai_database(db_name)
+	database.activate_connection()
+	database.load_genome_index()
+	database.load_accessions()
+	database.close_connection()
+	
+	#formatted_dataset = [(database, file.basename, file.best_hits_kmers, os.path.normpath(output+"/results")) for file in prepared_files] 
+	
+	#global accession_index
+	accession_index = generate_accessions_index()
+	
+	#Translate to indicies.
+	query_accessions_detected = [accession_index[a] for a in query_accessions_detected]
+	
+	#global target_kmer_cts
+	target_kmer_cts = {}
+	
+	for accession in np.intersect1d(database.accessions, query_accessions_detected):
+		target_kmer_cts[accession] = np.zeros(len(database.genome_index), dtype = np.int16)
+		for g in database.gak:
+			if accession in database.gak[g]:
+				target_kmer_cts[accession][g] = database.gak[g][accession]
+	
+	#global target_protein_counts
+	target_protein_counts = np.zeros(len(database.gak), dtype = np.int16)
+	for g in database.gak:
+		target_protein_counts[g] = len(database.gak[g])
+	
+	database.gak = None
+	
+	formatted_dataset = [(database, file.basename, file.best_hits_kmers, os.path.normpath(output+"/results")) for file in prepared_files] 
+	
+	if verbose:
+		print("")
+		print("-"*100)
+		print("")
+	
+	count = 0
+	total = len(formatted_dataset)
+	
+	print("Beginning AAI calculation")
+	
+	#globals to pass... target_kmer_cts target_protein_counts
+	#Just remake these in the procs. kmer_index accession_index
+	
+	if verbose:
+		print("")
+	#progress bar - possible dangerous use of the return to line start sequence.
+		try:
+			percentage = 0
+			sys.stdout.write("Completion".rjust(3)+ ' |'+('#'*int(percentage/2)).ljust(50)+'| ' + ('%.2f'%percentage).rjust(7)+'% (Query genome ' + str(count) + " of " + str(total) + ' done at '+curtime()+' )\n')
+			sys.stdout.flush()
+			last_pct = 0
+		except:
+			#It's not really a big deal if the progress bar cannot be printed.
+			pass
+	
+	#If parallelized, do parallel
+
+	pool = multiprocessing.Pool(threads, initializer = sql_query_thread_starter, initargs = (target_kmer_cts, target_protein_counts,))
+		
+	#Process as we go.
+	if do_stdev:
+		for file in pool.imap(do_sql_query, formatted_dataset):
+			
+			'''
+			handle = open(file, "r")
+			
+			for line in handle:
+				final_result.write(line)
+				
+			handle.close()
+			os.remove(file)	
+			'''
+			if verbose:
+			#progress bar - possible dangerous use of the return to line start sequence.
+				try:
+					count += 1
+					percentage = (count/total)*100
+					if int(percentage/2) > last_pct or count == total:
+						sys.stdout.write('\033[A')
+						sys.stdout.write("Completion".rjust(3)+ ' |'+('#'*int(percentage/2)).ljust(50)+'| ' + ('%.2f'%percentage).rjust(7)+'% (Query genome ' + str(count) + " of " + str(total) + ' done at '+curtime()+' )\n')
+						sys.stdout.flush()
+					last_pct = int(percentage/2)
+				except:
+					#It's not really a big deal if the progress bar cannot be printed.
+					pass
+		
+		pool.close()
+		pool.join()
+	else:
+
+		for file in pool.imap(do_sql_query_no_SD, formatted_dataset):
+			'''
+			handle = open(file, "r")
+			
+			for line in handle:
+				final_result.write(line)
+				
+			handle.close()
+			os.remove(file)	
+			'''
+			if verbose:
+			#progress bar - possible dangerous use of the return to line start sequence.
+				try:
+					count += 1
+					percentage = (count/total)*100
+					if int(percentage/2) > last_pct or count == total:
+						sys.stdout.write('\033[A')
+						sys.stdout.flush()
+						sys.stdout.write("Completion".rjust(3)+ ' |'+('#'*int(percentage/2)).ljust(50)+'| ' + ('%.2f'%percentage).rjust(7)+'% (Query genome ' + str(count) + " of " + str(total) + ' done at '+curtime()+' )\n')
+						sys.stdout.flush
+					last_pct = int(percentage/2)
+				except:
+					#It's not really a big deal if the progress bar cannot be printed.
+					pass
+		
+		pool.close()
+		pool.join()
+	
+	if verbose:
+		print("")
+		print("-"*100)
+		print("")
+		
+	if os.path.exists(output+"/temp"):
+		os.rmdir(output+"/temp")
+		
+	print("FastAAI query complete! Results at:", os.path.normpath(output + "/results"))
+	return None
+		
+
+#Check to see if the file exists and is a valid fastAAI db
+def assess_db(path):
+	status = None
+	if os.path.exists(path):
+		db = fastaai_database(path)
+		try:
+			db.activate_connection()
+			sql = "SELECT name FROM sqlite_master WHERE type='table'"
+			
+			db.cursor.row_factory = lambda cursor, row: row[0]
+			tables = db.cursor.execute(sql).fetchall()
+			db.cursor.row_factory = None
+			
+			db.close_connection()
+						
+			if len(tables) > 2 and "genome_index" in tables and "genome_acc_kmer_counts" in tables:		
+				status = "exists"
+			else:
+				status = "wrong format"
+			
+		except:
+			status = "wrong format"
+		
+	else:
+		try:
+			db = fastaai_database(path)
+			db.activate_connection()
+			db.initialize_parent_database()
+			db.close_connection()
+			status = "created"
+		except:
+			status = "unable to create"
+			
+	return status
+	
+#Add one FastAAI DB to another FastAAI DB
+def merge_db_opts():
+	parser = argparse.ArgumentParser(formatter_class=argparse.RawTextHelpFormatter,
+			description='''
+	This FastAAI module allows you to add the contents of one or more FastAAI databases to another.
+	You must have at least two already-created FastAAI databases using the build_db module before this module can be used.
+	
+	Supply a comma-separated list of at least one donor database and a single recipient database. 
+	If the recipient already exists, then genomes in all the donors will be added to the recipient.
+	If the recipient does not already exist, a new database will be created, and the contents of all the donors will be added to it.
+	
+	Example:
+	FastAAI.py merge_db --donors databases/db1.db,databases/db2.db -recipient databases/db3.db --threads 3
+	This command will create a new database called "db3.db", merge the data in db1.db and db2.db, and then add the merged data into db3.db
+	
+	Only the recipient database will be modified; the donors will be left exactly as they were before running this module.
+	''')
+			
+	parser.add_argument('-d', '--donors',      dest = 'donors',    default = None,   help =  'Comma-separated string of paths to one or more donor databases. The genomes FROM the donors will be added TO the recipient and the donors will be unaltered')
+
+	parser.add_argument('--donor_file',        dest = 'donor_file',    default = None,   help =  'Alternative way to supply donors. A file containing paths to the donor databases, 1 per line')
+
+	parser.add_argument('-r', '--recipient',   dest = 'recipient', default = None, help =  'Path to the recipient database. Any genomes FROM the donor database not already in the recipient will be added to this database.')	
+		
+	parser.add_argument('--verbose',           dest = 'verbose',   action='store_true', help = 'Print minor updates to console. Major updates are printed regardless.')
+	
+	parser.add_argument('--threads',      dest = 'threads', type=int, default = 1, help = 'The number of processors to use. Default 1.')
+
+	args, unknown = parser.parse_known_args()
+	
+	return parser, args
+	
+def merge_db_thread_starter(rev_index, per_db_accs):
+	global reverse_genome_indicies
+	global accs_per_db	
+	reverse_genome_indicies = rev_index
+	accs_per_db = per_db_accs
+	
+	
+	
+def merge_db(recipient, donors, donor_file, verbose, threads):
+	#Prettier on the CLI
+	
+	if donor_file is not None:
+		fh = agnostic_reader(donor_file)
+		donors = [line.strip() for line in fh]
+		fh.close()
+	
+	if donors is None or recipient is None:
+		print("Either donor or target not given. FastAAI is exiting.")
+		return None
+	
+	print("")
+	
+	if donor_file is None:
+		donors = donors.split(",")
+	
+	valid_donors = []
+	for d in donors:
+		if os.path.exists(d):
+			if d == recipient:
+				print("Donor database", d, "is the same as the recipient. This database will be skipped.")
+			else:
+				check = assess_db(d)
+				if check == "exists":
+					if d not in valid_donors:
+						valid_donors.append(d)
+					else:
+						print("It appears that database", d, "was already added to the list of donors. Did you type it twice in the list of donors? Skipping it.")
+				else:
+					if check == "created":
+						print("Donor database", d, "not found! Skipping.")
+					else:
+						print("Something was wrong with supplied database:", d+". A status check found:", check)
+		else:
+			print("Donor database", d, "not found! Are you sure the path is correct and this donor exists? This database will be skipped.")
+	
+	if len(valid_donors) == 0:
+		print("None of the supplied donor databases were able to be accessed. FastAAI cannot continue if none of these databases are valid. Exiting.")
+		sys.exit()
+		
+	recip_check = assess_db(recipient)
+		
+	if recip_check == "created" or recip_check == "exists":
+		for donor in valid_donors:
+			print("Donor database:", donor, "will be added to recipient database:", recipient)
+			
+		recipient = fastaai_database(recipient)
+	else:
+		print("I couldn't find or create the recipient database at", recipient+".", "Does the folder you're trying to place this database in exist, and do you have permission to write files to it? FastAAI exiting.")
+		sys.exit()
+		
+	if recipient is None or len(valid_donors) == 0:
+		print("I require both a valid donor and a recipient database. FastAAI exiting.")
+		sys.exit()
+
+	donor_dbs = []
+	for d in valid_donors:
+		donor_dbs.append(fastaai_database(d))
+		
+	all_accessions = set()
+	#global joint_genome_index
+	joint_genome_index = {}
+	joint_genome_counts = {}
+	max_index = 0
+	#The idea here is to create a set of arrays whose values span the range of each donor's genomes and translate those into an overall list, in order.
+	
+	#global reverse_genome_indicies
+	reverse_genome_indices = {}
+	
+	#global accs_per_db
+	accs_per_db = {}
+		
+	#Load recipient data, if any.
+	if recip_check == "exists":
+		recipient.activate_connection()
+		recipient.just_accessions()
+		recipient.load_genome_index()
+		recipient.close_connection()
+		
+		all_accessions = all_accessions.union(recipient.accessions)
+		accs_per_db[recipient.path] = recipient.accessions
+		recipient.accessions = None
+		max_index = len(recipient.genome_index)
+		
+		joint_genome_index = dict(zip(recipient.genome_index.keys(), recipient.genome_index.values()))
+		joint_genome_counts = dict(zip(recipient.protein_counts_by_genome.keys(), recipient.protein_counts_by_genome.values()))
+		
+		#reverse_genome_index = dict(zip(joint_genome_index.values(),joint_genome_index.keys()))
+		#So... the keys are the genome indicies of the recip. These... shouldn't need any updates. Only the donors need to match.
+		ct = 0
+		path = recipient.path
+		reverse_genome_indices[path] = []
+		for idx in sorted(recipient.genome_index.values()):
+			reverse_genome_indices[path].append(idx)
+		reverse_genome_indices[path] = np.array(reverse_genome_indices[path], dtype = np.int32)
+		recipient.genome_index = None
+	
+	#Donors should always exist, never be created.
+	for d in donor_dbs:
+		d.activate_connection()
+		d.just_accessions()
+		d.load_genome_index()
+		d.close_connection()
+		accs_per_db[d.path] = d.accessions
+		all_accessions = all_accessions.union(d.accessions)
+		d.accessions = None
+		reverse_genome_indices[d.path] = []
+		#Database construction indicates this should always be 0-COUNT
+		for g in sorted(d.genome_index.keys()):
+			if g not in joint_genome_index:
+				reverse_genome_indices[d.path].append(max_index)
+				joint_genome_index[g] = max_index
+				#Map the counts on.
+				joint_genome_counts[max_index] = d.protein_counts_by_genome[d.genome_index[g]]
+				#reverse_genome_index[max_index] = g
+				max_index += 1
+			else:
+				reverse_genome_indices[d.path].append(joint_genome_index[g])
+		#Make it an array, now
+		reverse_genome_indices[d.path] = np.array(reverse_genome_indices[d.path], dtype = np.int32)
+		d.genome_index = None
+	
+	#global accession_index
+	accession_index = generate_accessions_index()
+	
+	#global accession_inverter
+	accession_inverter = {}
+	for acc in accession_index:
+		sql_friendly_accession = acc.replace(".", "_")
+		accession_inverter[accession_index[acc]] = sql_friendly_accession
+		
+	all_accessions = list(all_accessions)
+	
+	
+	print("")
+	print("Formatting data to add to database. Started at", curtime())
+	
+	temp_dir = tempfile.mkdtemp()
+	try:
+		acc_args = [(acc, donor_dbs, recipient, temp_dir) for acc in all_accessions]
+	
+		if verbose:
+			print("")
+			count = 0
+			total_counts = len(acc_args)
+			try:
+				percentage = (count/total_counts)*100
+				sys.stdout.write("Completion".rjust(3)+ ' |'+('#'*int(percentage/2)).ljust(50)+'| ' + ('%.2f'%percentage).rjust(7)+'% ( ' + str(count) + " of " + str(total_counts) + ' done at ' + curtime() + " )\n")
+				sys.stdout.flush()			
+			except:
+				#It's not really a big deal if the progress bar cannot be printed.
+				pass
+		
+		last_pct = 0
+		
+		pool = multiprocessing.Pool(threads, initializer=merge_db_thread_starter, initargs = (reverse_genome_indices, accs_per_db,))
+		
+		quiverfull = []
+		for result in pool.imap_unordered(pull_and_merge_accession, acc_args):
+			acc = result[0]
+			child = result[1]
+			#sub_gak = result[2]
+			
+			quiverfull.append([acc, child])
+			#gaks.extend(sub_gak)
+			
+			if verbose:
+				count += 1
+				try:
+					percentage = (count/total_counts)*100
+					log_time = curtime()
+					sys.stdout.write('\033[A')
+					sys.stdout.flush()
+					sys.stdout.write("Completion".rjust(3)+ ' |'+('#'*int(percentage/2)).ljust(50)+'| ' + ('%.2f'%percentage).rjust(7)+'% ( ' + str(count) + " of " + str(total_counts) + ' done at ' + curtime() + " )\n")
+					sys.stdout.flush()			
+				except:
+					#It's not really a big deal if the progress bar cannot be printed.
+					pass
+		
+		pool.close()
+		pool.join()
+		
+		print("")
+		print("Adding data to final database. Started at", curtime())
+		
+		if verbose:
+			print("")
+			
+			count = 0
+			total_counts = len(acc_args)
+			try:
+				percentage = (count/total_counts)*100
+				sys.stdout.write("Completion".rjust(3)+ ' |'+('#'*int(percentage/2)).ljust(50)+'| ' + ('%.2f'%percentage).rjust(7)+'% ( ' + str(count) + " of " + str(total_counts) + ' done at ' + curtime() + " )\n")
+				sys.stdout.flush()
+			except:
+				#It's not really a big deal if the progress bar cannot be printed.
+				pass
+		
+		last_pct = 0
+		
+		recipient.activate_connection()
+		genome_list_update_sql = "INSERT OR REPLACE INTO genome_index VALUES (?, ?, ?)"
+		genome_reindex = []
+		for g in joint_genome_index:
+			genome_reindex.append((g, joint_genome_index[g], joint_genome_counts[joint_genome_index[g]]))
+			
+		recipient.cursor.executemany(genome_list_update_sql, genome_reindex)
+		recipient.connection.commit()
+		
+		del genome_reindex
+		
+		for result in quiverfull:
+			acc = result[0]
+			child = result[1]
+			
+			recipient.add_child_to_parent(acc, child, genomes_too = True, update_gak = True)
+			
+			if verbose:
+				count += 1
+				try:
+					percentage = (count/total_counts)*100
+					log_time = curtime()
+					sys.stdout.write('\033[A')
+					sys.stdout.flush()
+					sys.stdout.write("Completion".rjust(3)+ ' |'+('#'*int(percentage/2)).ljust(50)+'| ' + ('%.2f'%percentage).rjust(7)+'% ( ' + str(count) + " of " + str(total_counts) + ' done at ' + curtime() + " )\n")
+					sys.stdout.flush()
+				except:
+					#It's not really a big deal if the progress bar cannot be printed.
+					pass
+	except:
+		#Error
+		if os.path.exists(temp_dir):
+			shutil.rmtree(temp_dir)
+	finally:
+		#Success
+		if os.path.exists(temp_dir):
+			shutil.rmtree(temp_dir)
+		
+	print("\nDatabases merged!")
+	
+	return None
+	
+def pull_and_merge_accession(args):
+	accession_index = generate_accessions_index()
+	
+	#global accession_inverter
+	accession_inverter = {}
+	for acc in accession_index:
+		sql_friendly_accession = acc.replace(".", "_")
+		accession_inverter[accession_index[acc]] = sql_friendly_accession
+	
+	#joint_genome_index, accession_index, accession_inverter, accs_per_db are global already.
+	acc, donor_dbs, recipient, temp = args[0], args[1], args[2], args[3]
+	
+	acc_name = accession_inverter[acc]
+	acc_name_gens = acc_name + "_genomes"
+	
+	query_sql = "SELECT * FROM " + acc_name
+	
+	temp_db = fastaai_database(os.path.normpath(temp+"/"+acc_name+".db"))
+	temp_db.activate_connection()
+	
+	create_command = "CREATE TABLE IF NOT EXISTS " + acc_name + " (kmer INTEGER PRIMARY KEY, genomes array)"
+	temp_db.cursor.execute(create_command)
+	temp_db.connection.commit()
+	
+	create_command = "CREATE TABLE IF NOT EXISTS " + acc_name + "_genomes (genome INTEGER PRIMARY KEY, kmers array)"
+	temp_db.cursor.execute(create_command)
+	temp_db.connection.commit()
+	
+	query_lists = {}
+	for db in donor_dbs:
+		if acc in accs_per_db[db.path]:
+			db.activate_connection()
+			
+			for result in db.cursor.execute(query_sql).fetchall():
+				kmer = result[0]
+				genomes = result[1]
+				translated_genomes = reverse_genome_indicies[db.path][genomes]
+				
+				if kmer in query_lists:
+					query_lists[kmer] = np.union1d(query_lists[kmer], translated_genomes)
+				else:
+					query_lists[kmer] = translated_genomes
+			
+			db.close_connection()
+	
+	#Recipient is not guaranteed to be in the accs per db - if it was created anew, it wouldn't be.
+	if recipient.path in accs_per_db:
+		if acc in accs_per_db[recipient.path]:
+			recipient.activate_connection()	
+			
+			for result in recipient.cursor.execute(query_sql).fetchall():
+				kmer = result[0]
+				genomes = result[1]
+				translated_genomes = reverse_genome_indicies[recipient.path][genomes]
+				if kmer in query_lists:
+					query_lists[kmer] = np.union1d(query_lists[kmer], translated_genomes)
+				else:
+					query_lists[kmer] = translated_genomes
+			
+			recipient.close_connection()
+	
+	#Byte-string these.
+	for kmer in query_lists:
+		query_lists[kmer] = query_lists[kmer].tobytes()
+	
+	temp_db.cursor.executemany("INSERT INTO " + acc_name + " VALUES (?,?)", zip(query_lists.keys(), query_lists.values()))
+	temp_db.connection.commit()
+	
+	del query_lists
+	
+	#Reset. Do genomes
+	query_genomes_sql = "SELECT * FROM " + acc_name_gens
+	query_lists = {}
+	for db in donor_dbs:
+		if acc in accs_per_db[db.path]:
+			db.activate_connection()
+			
+			for result in db.cursor.execute(query_genomes_sql).fetchall():
+				genome = result[0]
+				kmers = result[1]
+				translated_genome = int(reverse_genome_indicies[db.path][genome])
+				#Each genome gets added only once, no dupes.
+				if translated_genome not in query_lists:
+					query_lists[translated_genome] = kmers
+
+			db.close_connection()
+	
+	if recipient.path in accs_per_db:
+		if acc in accs_per_db[recipient.path]:
+			recipient.activate_connection()	
+			
+			for result in recipient.cursor.execute(query_genomes_sql).fetchall():
+				genome = result[0]
+				kmers = result[1]
+				translated_genome = int(reverse_genome_indicies[recipient.path][genome])
+				#Each genome gets added only once, no dupes.
+				if translated_genome not in query_lists:
+					query_lists[translated_genome] = kmers
+			
+			recipient.close_connection()
+	
+	#Byte-string these.
+	#gak = []
+	for g in query_lists:
+		#gak.append((g, acc, query_lists[g].shape[0]))
+		query_lists[g] = query_lists[g].tobytes()
+		
+	
+	temp_db.cursor.executemany("INSERT INTO " + acc_name_gens + " VALUES (?,?)", zip(query_lists.keys(), query_lists.values()))
+	temp_db.connection.commit()
+	
+	temp_db.close_connection()
+	
+	return [acc_name, temp_db.path]
+
+#Query 1 genome vs. 1 target using Carlos' method - just needs query, target, threads
+def single_query_opts():
+	parser = argparse.ArgumentParser(formatter_class=argparse.RawTextHelpFormatter,
+			description='''
+	This FastAAI module takes a single query genome, protein, or protein and HMM pair and a single target genome, protein, or protein and HMM pair as inputs and calculates AAI between the two.
+	
+	If you supply a genome as either query or target, a protein and HMM file will be made for the genome.
+	If you supply a protein as either query or target, an HMM file will be made for it.
+	If you supply both an HMM and protein, the search will start right away. You cannot provide only an HMM.
+	
+	No database will be built, and you cannot query multiple genomes with this module.
+	
+	If you wish to query multiple genomes against themselves in all vs. all AAI search, use aai_index instead.
+	If you wish to query multiple genomes against multiple targets, use multi_query instead.
+	''')
+	parser.add_argument('-qg', '--query_genome',  dest = 'query_genome',  default = None, help =  'Query genome')
+	parser.add_argument('-tg', '--target_genome', dest = 'target_genome', default = None, help =  'Target genome')
+	
+	parser.add_argument('-qp', '--query_protein',  dest = 'query_protein',  default = None, help =  'Query protein')
+	parser.add_argument('-tp', '--target_protein', dest = 'target_protein', default = None, help =  'Target protein')
+	
+	parser.add_argument('-qh', '--query_hmm',  dest = 'query_hmm',  default = None, help =  'Query HMM')
+	parser.add_argument('-th', '--target_hmm', dest = 'target_hmm', default = None, help =  'Target HMM')
+	
+	parser.add_argument('-o', '--output', dest = 'output', default = "FastAAI", help = 'The directory where FastAAI will place the result of this query. By default, a directory named "FastAAI" will be created in the current working directory and results will be placed there.')	
+	
+	parser.add_argument('--threads',  dest = 'threads', type=int, default = 1, help = 'The number of processors to use. Default 1.')
+	parser.add_argument('--verbose',        dest = 'verbose', action='store_true', help =   'Print minor updates to console. Major updates are printed regardless.')
+
+	#Alternative file input
+	
+	args, unknown = parser.parse_known_args()
+	
+	return parser, args
+
+def do_single_query(input_file):
+	input_file.preprocess()
+	return input_file
+	
+def intersect_kmer_lists(pair):
+	intersection = np.intersect1d(pair[0], pair[1]).shape[0]
+	union = pair[0].shape[0] + pair[1].shape[0] - intersection
+	return (intersection/union)
+	
+def kaai_to_aai(kaai):
+	# Transform the kAAI into estimated AAI values
+	aai_hat = (-0.3087057 + 1.810741 * (np.exp(-(-0.2607023 * np.log(kaai))**(1/3.435))))*100
+	
+	return aai_hat
+	
+#This one's unique. It doesn't do anything with the DB, which means it doesn't access any other functionality outside of the input_file class. It just advances a pair of inputs in parallel and does intersections.
+def single_query(query_args, target_args, shared_args):
+	
+	output, threads, verbose = shared_args[0], shared_args[1], shared_args[2]
+
+	genomes, proteins, hmms = query_args[0], query_args[1], query_args[2]
+	
+	if genomes is None and proteins is None and hmms is None:
+		print("Please supply a query genome, protein, or protein and HMM pair.")
+		sys.exit()
+	
+	query = None
+	
+	if genomes is not None:
+		query = input_file(genomes, output, verbose)
+		query.set_genome(genomes)
+	if proteins is not None:
+		if query is not None:
+			print("If you supply a genome for either query or target, you must supply ONLY the genome, not a genome and either a protein or HMM.")
+			sys.exit()
+		else:
+			query = input_file(proteins, output, verbose)
+			query.set_protein(proteins)
+	if hmms is not None:
+		if query is None:
+			print("If you supply an HMM for either query or target, you must also supply the protein from which the HMM was generated.")
+			sys.exit()
+		else:
+			query.set_hmm(hmms)
+		
+	genomes, proteins, hmms = target_args[0], target_args[1], target_args[2]
+
+	if genomes is None and proteins is None and hmms is None:
+		print("Please supply a target genome, protein, or protein and HMM pair.")
+		sys.exit()
+	
+	target = None
+	
+	if genomes is not None:
+		target = input_file(genomes, output, verbose)
+		target.set_genome(genomes)
+	if proteins is not None:
+		if target is not None:
+			print("If you supply a genome for either target or target, you must supply ONLY the genome, not a genome and either a protein or HMM.")
+			sys.exit()
+		else:
+			target = input_file(proteins, output, verbose)
+			target.set_protein(proteins)
+	if hmms is not None:
+		if target is None:
+			print("If you supply an HMM for either target or target, you must also supply the protein from which the HMM was generated.")
+			sys.exit()
+		else:
+			target.set_hmm(hmms)
+		
+	if query.basename == target.basename:
+		print("You've selected the same query and target genome. The AAI is 100%.")
+		print("FastAAI exiting.")
+		return None
+		
+	statuses = ["genome", "protein", "protein and hmm"]
+	query_stat = statuses.index(query.status)
+	target_stat = statuses.index(target.status)
+	minimum_status = statuses[min(query_stat, target_stat)]
+	
+	start_printouts = ["[Genome] Protein Protein+HMM", " Genome [Protein] Protein+HMM", "Genome Protein [Protein+HMM]"]
+	
+	print("")
+	print("Query start: ", start_printouts[query_stat])
+	print("Target start:", start_printouts[target_stat])
+	print("")
+	
+	good_to_go = prepare_directories(output, minimum_status, "build")
+	
+	if not good_to_go:
+		print("Exiting FastAAI")
+		sys.exit()	
+	
+	qname = query.basename
+	tname = target.basename
+	
+	name = qname + "_vs_" + tname + ".aai.txt"
+	print("Output will be located at", os.path.normpath(output) + "/results/"+name)
+	
+	#Give the data for kmer indexing to the parallel processes
+	global kmer_index
+	kmer_index = create_kmer_index()
+	
+	advance_me = [query, target]
+	#All we need to do this.
+	pool = multiprocessing.Pool(min(threads, 2))
+	
+	results = pool.map(do_single_query, advance_me)
+	
+	pool.close()
+	pool.join()
+	
+	query = results[0]
+	target = results[1]
+	
+	#One of the printouts
+	max_poss_prots = max(len(query.best_hits_kmers), len(target.best_hits_kmers))
+	
+	accs_to_view = set(query.best_hits_kmers.keys()).intersection(set(target.best_hits_kmers.keys()))
+	
+	seq_pairs = [[query.best_hits_kmers[acc], target.best_hits_kmers[acc]] for acc in accs_to_view]
+	
+	pool = multiprocessing.Pool(min(threads, len(accs_to_view)))
+	
+	results = np.array(pool.map(intersect_kmer_lists, seq_pairs))
+	
+	pool.close()
+	pool.join()
+	
+	jacc_mean = np.mean(results)
+	jacc_std = np.std(results)
+	actual_prots = len(results)
+	aai_est = round(kaai_to_aai(jacc_mean), 2)
+	
+	if aai_est > 90:
+		aai_est = "> 90%"
+	else:
+		if aai_est < 30:
+			aai_est = "< 30%"
+	
+	output = open(name, "w")
+	
+	print(qname, tname, round(jacc_mean, 4), round(jacc_std, 4), actual_prots, aai_est, file = output)
+	
+	output.close()
+	
+	print("FastAAI single query done! Estimated AAI:", aai_est)
+	
+def aai_index_opts():
+	parser = argparse.ArgumentParser(formatter_class=argparse.RawTextHelpFormatter,
+			description='''
+	This FastAAI module takes a set of genomes, proteins, or proteins and HMMs, creates a FastAAI database from them, and then executes an all vs. all AAI search of the genomes in the database
+	''')
+	
+	parser.add_argument('-g', '--genomes',  dest = 'genomes', default = None, help =  'A directory containing genomes in FASTA format.')
+	parser.add_argument('-p', '--proteins', dest = 'proteins', default = None, help = 'A directory containing protein amino acids in FASTA format.')
+	parser.add_argument('-m', '--hmms',     dest = 'hmms', default = None, help =     'A directory containing the results of an HMM search on a set of proteins.')
+	
+	parser.add_argument('-d', '--database', dest = 'db_name', default = "FastAAI_database.sqlite.db", help =  'The name of the database you wish to create or add to. The database will be created if it doesn\'t already exist and placed in the output directory.')
+	
+	parser.add_argument('-o', '--output',   dest = 'output', default = "FastAAI", help = 'The directory to place the database and any protein or HMM files FastAAI creates. By default, a directory named "FastAAI" will be created in the current working directory and results will be placed there.')
+		
+	parser.add_argument('--genome_file',    dest = 'gf', default = None, help =  'Alternative way to supply genomes. A file containing paths to your genome files, 1 per line.')
+	parser.add_argument('--protein_file',   dest = 'pf', default = None, help = 'Alternative way to supply proteins. A file containing paths to your protein files, 1 per line.')
+	parser.add_argument('--hmm_file',       dest = 'hf', default = None, help =     'Alternative way to supply HMMs. A file containing paths to your HMM files, 1 per line.')
+		
+	parser.add_argument('--verbose',        dest = 'verbose', action='store_true', help = 'Print minor updates to console. Major updates are printed regardless.')
+	parser.add_argument('--threads',  dest = 'threads', type=int, default = 1, help = 'The number of processors to use. Default 1.')
+
+	
+	parser.add_argument('--do_stdev',   dest = "do_stdev", action='store_true',                 help = 'Off by default. Calculate std. deviations on Jaccard indicies. Increases memory usage and runtime slightly. Does NOT change estimated AAI values at all.')
+	parser.add_argument('--unlimited_resources', dest = "large_mem",  action = 'store_true', help = 'Off by default. Use a faster algorithm that consumes more RAM. FastAAI cannot calculate std. deviations with this algorithm, so they will automatically be skipped.')
+	parser.add_argument('--mem',        dest = "precision", default = "med",                 help = 'One of low/med/high. Medium by default. Save RAM in return for slightly rounded AAI estimates. Only affects FastAAI if you are also using the "--unlimited_resources" flag.')
+	
+	args, unknown = parser.parse_known_args()
+	
+	return parser, args
+
+#Build a DB and query a dataset vs. self
+def aai_index(genomes, proteins, hmms, db_name, output, threads, gf, pf, hf, verbose, do_stdev, memory_use, unlimited_resources):
+	#run build DB and then db_query with the fresh DB
+	success = build_db(genomes, proteins, hmms, db_name, output, threads, gf, pf, hf, verbose)
+	if success:
+		accessible_name = os.path.normpath(output + "/database/" + db_name)
+		db_query(accessible_name, accessible_name, verbose, output, threads, do_stdev, memory_use, unlimited_resources)
+	else:
+		print("Database could not be built. FastAAI exiting.")
+		
+	return None
+	
+#Build 2 DBs and query query DB vs target DB
+def multi_query_opts():
+	parser = argparse.ArgumentParser(formatter_class=argparse.RawTextHelpFormatter,
+			description='''
+	This FastAAI module takes a set of query genomes/proteins/proteins+HMMs and a set of target genomes/proteins/proteins+HMMs.
+	Two FastAAI databases will be created, one for the query and one for the target, then the query database will have AAI calculated against the target database
+	''')
+
+	parser.add_argument('-qg', '--query_genomes',  dest = 'query_genomes', default = None, help =  'A directory containing query genomes in FASTA format.')
+	parser.add_argument('-qp', '--query_proteins', dest = 'query_proteins', default = None, help = 'A directory containing query protein amino acids in FASTA format.')
+	parser.add_argument('-qm', '--query_hmms',     dest = 'query_hmms', default = None, help =     'A directory containing the results of an HMM search on the set of query proteins.')
+	
+	parser.add_argument('-tg', '--target_genomes',  dest = 'target_genomes', default = None, help =  'A directory containing target genomes in FASTA format.')
+	parser.add_argument('-tp', '--target_proteins', dest = 'target_proteins', default = None, help = 'A directory containing target protein amino acids in FASTA format.')
+	parser.add_argument('-tm', '--target_hmms',     dest = 'target_hmms', default = None, help =     'A directory containing the results of an HMM search on the set of target proteins.')
+	
+	
+	parser.add_argument('-qd', '--query_database', dest = 'query_db_name', default =   "FastAAI_query_database.sqlite.db", help =  'The name of the query database you wish to create or add to. The database will be created if it doesn\'t already exist and placed in the output directory.')
+	parser.add_argument('-td', '--target_database', dest = 'target_db_name', default = "FastAAI_target_database.sqlite.db", help =  'The name of the target database you wish to create or add to. The database will be created if it doesn\'t already exist and placed in the output directory.')
+	
+	parser.add_argument('-o', '--output',   dest = 'output', default = "FastAAI", help = 'The directory to place the database and any protein or HMM files FastAAI creates. By default, a directory named "FastAAI" will be created in the current working directory and results will be placed there.')
+						
+	parser.add_argument('--query_genome_file',    dest = 'qgf', default = None, help =  'Alternative way to supply genomes. A file containing paths to your query genome files, 1 per line.')
+	parser.add_argument('--query_protein_file',   dest = 'qpf', default = None, help = 'Alternative way to supply proteins. A file containing paths to your query protein files, 1 per line.')
+	parser.add_argument('--query_hmm_file',       dest = 'qhf', default = None, help =     'Alternative way to supply HMMs. A file containing paths to your query HMM files, 1 per line.')
+		
+	parser.add_argument('--target_genome_file',    dest = 'tgf', default = None, help =  'Alternative way to supply genomes. A file containing paths to your target genome files, 1 per line.')
+	parser.add_argument('--target_protein_file',   dest = 'tpf', default = None, help = 'Alternative way to supply proteins. A file containing paths to your target protein files, 1 per line.')
+	parser.add_argument('--target_hmm_file',       dest = 'thf', default = None, help =     'Alternative way to supply HMMs. A file containing paths to your target HMM files, 1 per line.')
+	
+	parser.add_argument('--threads',  dest = 'threads', type=int, default = 1, help = 'The number of processors to use. Default 1.')
+	parser.add_argument('--verbose',        dest = 'verbose', action='store_true', help = 'Print minor updates to console. Major updates are printed regardless.')
+
+	parser.add_argument('--do_stdev',   dest = "do_stdev", action='store_true',                 help = 'Off by default. Calculate std. deviations on Jaccard indicies. Increases memory usage and runtime slightly. Does NOT change estimated AAI values at all.')
+	parser.add_argument('--unlimited_resources', dest = "large_mem",  action = 'store_true', help = 'Off by default. Use a faster algorithm that consumes more RAM. FastAAI cannot calculate std. deviations with this algorithm, so they will automatically be skipped.')
+	parser.add_argument('--mem',        dest = "precision", default = "med",                 help = 'One of low/med/high. Medium by default. Save RAM in return for slightly rounded AAI estimates. Only affects FastAAI if you are also using the "--unlimited_resources" flag.')
+	
+	args, unknown = parser.parse_known_args()
+	
+	return parser, args
+
+#Build 2 DBs and query query DB vs target DB
+def multi_query(query_arg_list, target_arg_list, shared_args):
+	pass
+	output, threads, verbose, do_stdev, mem, efficient = shared_args[0], shared_args[1], shared_args[2], shared_args[3], shared_args[4], shared_args[5]
+	
+	genomes, proteins, hmms, gf, pf, hf, db_name = query_arg_list[0], query_arg_list[1], query_arg_list[2], query_arg_list[3], query_arg_list[4], query_arg_list[5], query_arg_list[6]
+	accessible_name_query = os.path.normpath(output + "/database/" + db_name)
+	build_db(genomes, proteins, hmms, db_name, output, threads, gf, pf, hf, verbose)
+	
+	genomes, proteins, hmms, gf, pf, hf, db_name = target_arg_list[0], target_arg_list[1], target_arg_list[2], target_arg_list[3], target_arg_list[4], target_arg_list[5], target_arg_list[6]
+	accessible_name_target = os.path.normpath(output + "/database/" + db_name)
+	build_db(genomes, proteins, hmms, db_name, output, threads, gf, pf, hf, verbose)
+	
+	db_query(accessible_name_query, accessible_name_target, verbose, output, threads, do_stdev, mem, efficient)
+
+'''
+Main
+'''
+def main():
+	#The currently supported modules.
+	modules = ["build_db", "merge_db", "simple_query", "db_query", "single_query", "aai_index", "multi_query"]
+	
+	#Print modules if someone just types FastAAI
+	if len(sys.argv) < 2:
+		print("")
+		print("    Welcome to FastAAI")
+		print("")
+		print("")
+		print("    Please select one of the following modules:")
+		print("")
+		print("------------------------------------------- Quick Usage Options -------------------------------------------")
+		print("")
+		print("    single_query |" + " Quickly query ONE query genome against ONE target genome")
+		print("    multi_query  |" + " Create a query DB and a target DB, then calculate query vs. target AAI")
+		print("    aai_index    |" + " Create a database from multiple genomes and do an all vs. all AAI index of the genomes")
+		print("")
+		print("-------------------------------------- Database Construction Options --------------------------------------")
+		print("")
+		print("    build_db     |" + " Create or add to a FastAAI database from genomes, proteins, or proteins and HMMs")
+		print("    merge_db     |" + " Add the contents of one FastAAI DB to another")
+		print("")
+		print("---------------------------------------------- Query Options ----------------------------------------------")
+		print("")
+		print("    simple_query |" + " Query a genome or protein (one or many) against an existing FastAAI database")
+		print("    db_query     |" + " Query the genomes in one FastAAI database against the genomes in another FastAAI database")
+		print("")
+		print("-----------------------------------------------------------------------------------------------------------")
+		print("")
+		print("                    To select a module, enter 'FastAAI [module]' into the command line!")
+		print("")
+		sys.exit()
+		
+	#This is the module selection
+	selection = sys.argv[1]
+	
+	if selection not in modules:
+		print("")
+		print("    I couldn't find the module you specified. Please select one of the following modules:")
+		print("")
+		print("------------------------------------------- Quick Usage Options -------------------------------------------")
+		print("")
+		print("    single_query |" + " Quickly query ONE query genome against ONE target genome")
+		print("    multi_query  |" + " Create a query DB and a target DB, then calculate query vs. target AAI")
+		print("    aai_index    |" + " Create a database from multiple genomes and do an all vs. all AAI index of the genomes")
+		print("")
+		print("-------------------------------------- Database Construction Options --------------------------------------")
+		print("")
+		print("    build_db     |" + " Create or add to a FastAAI database from genomes, proteins, or proteins and HMMs")
+		print("    merge_db     |" + " Add the contents of one FastAAI DB to another")
+		print("")
+		print("---------------------------------------------- Query Options ----------------------------------------------")
+		print("")
+		print("    simple_query |" + " Query a genome or protein (one or many) against an existing FastAAI database")
+		print("    db_query     |" + " Query the genomes in one FastAAI database against the genomes in another FastAAI database")
+		print("")
+		print("-----------------------------------------------------------------------------------------------------------")
+		print("")
+		print("    To select a module, enter 'FastAAI [module]' into the command line!")
+		print("")
+		sys.exit()
+	
+#################### Database build or add ########################
+	
+	if selection == "build_db":
+		parser, opts = build_db_opts()
+		
+		#module name only
+		if len(sys.argv) < 3:
+			print(parser.print_help())
+			sys.exit()
+		
+		#Directory based
+		genomes, proteins, hmms = opts.genomes, opts.proteins, opts.hmms
+		
+		#Input list based
+		gf, pf, hf = opts.gf, opts.pf, opts.hf
+		
+		output  = os.path.normpath(opts.output)
+	
+		threads = opts.threads
+		verbose = opts.verbose
+		
+		#Database handle
+		db_name = opts.db_name
+		
+		
+		#genomes, proteins, hmms, db_name, output, threads, gf, pf, hf, verbose
+		build_db(genomes, proteins, hmms, db_name, output, threads, gf, pf, hf, verbose)
+		
+#################### Add two DBs ########################	
+
+	if selection == "merge_db":
+		parser, opts = merge_db_opts()
+		if len(sys.argv) < 3:
+			print(parser.print_help())
+			sys.exit()
+			
+		recipient = opts.recipient
+		donors = opts.donors
+		donor_file = opts.donor_file
+		verbose = opts.verbose
+		threads = opts.threads
+		
+		merge_db(recipient, donors, donor_file, verbose, threads)
+		
+#################### Query files vs DB ########################	
+		
+	if selection == "simple_query":
+		parser, opts = sql_query_opts()
+		
+		if len(sys.argv) < 3:
+			print(parser.print_help())
+			sys.exit()
+			
+		#directory based
+		genomes, proteins, hmms = opts.genomes, opts.proteins, opts.hmms
+		
+		#Input list based
+		gf, pf, hf = opts.gf, opts.pf, opts.hf
+		
+		db_name = opts.target
+		
+		output  = opts.output
+		threads = opts.threads
+		verbose = opts.verbose
+		
+		do_stdev = opts.do_stdev
+				
+		sql_query(genomes, proteins, hmms, gf, pf, hf, db_name, output, threads, verbose, do_stdev)
+		
+			
+#################### Query DB vs DB ###########################		
+	if selection == "db_query":
+		parser, opts = db_query_opts()
+		#module name only
+		
+		if len(sys.argv) < 3:
+			print(parser.print_help())
+			sys.exit()
+			
+		query = opts.query
+		target = opts.target
+		verbose = opts.verbose
+		
+		do_stdev = opts.do_stdev
+		#massive = opts.massive
+		
+		mem = opts.precision
+		efficient = opts.large_mem
+		
+		output = opts.output
+		threads = opts.threads
+		
+		db_query(query, target, verbose, output, threads, do_stdev, mem, efficient)
+
+#################### One-pass functions #######################
+	if selection == "single_query":
+		parser, opts = single_query_opts()
+		#module name only
+		
+		if len(sys.argv) < 3:
+			print(parser.print_help())
+			sys.exit()
+			
+		shared_opts = []
+		output = os.path.normpath(opts.output)
+		threads = opts.threads
+		verbose = opts.verbose
+		
+		shared_opts.append(output)
+		
+		shared_opts.append(threads)
+		shared_opts.append(verbose)
+		
+		query_opts = []
+		
+		query_genome = opts.query_genome
+		query_protein = opts.query_protein
+		query_hmm = opts.query_hmm
+		
+		
+		query_opts.append(query_genome)
+		query_opts.append(query_protein)
+		query_opts.append(query_hmm)
+		
+		target_opts = []
+		
+		target_genome = opts.target_genome
+		target_protein = opts.target_protein
+		target_hmm = opts.target_hmm
+		
+		#tg = opts.target_genome_file
+		#tp = opts.target_protein_file
+		#th = opts.target_hmm_file
+		
+		target_opts.append(target_genome)
+		target_opts.append(target_protein)
+		target_opts.append(target_hmm)
+			
+		single_query(query_opts, target_opts, shared_opts)
+		
+	if selection == "aai_index":
+		parser, opts = aai_index_opts()
+		#module name only
+		
+		if len(sys.argv) < 3:
+			print(parser.print_help())
+			sys.exit()
+			
+			
+		genomes, proteins, hmms = opts.genomes, opts.proteins, opts.hmms
+		#Text file versions of genomes/proteins/hmms
+		gf, pf, hf = opts.gf, opts.pf, opts.hf
+		
+		db_name = opts.db_name
+		
+		output = opts.output
+		threads = opts.threads
+		verbose = opts.verbose
+		
+		do_stdev = opts.do_stdev
+		#massive = opts.massive
+		
+		mem = opts.precision
+		efficient = opts.large_mem
+		
+		aai_index(genomes, proteins, hmms, db_name, output, threads, gf, pf, hf, verbose, do_stdev, mem, efficient)
+	
+	if selection == "multi_query":
+		parser, opts = multi_query_opts()
+		#module name only
+		
+		if len(sys.argv) < 3:
+			print(parser.print_help())
+			sys.exit()
+			
+		shared_arg_list = []
+		output = os.path.normpath(opts.output)
+		threads = opts.threads
+		verbose = opts.verbose
+		
+		do_stdev = opts.do_stdev
+		#massive = opts.massive
+		
+		mem = opts.precision
+		efficient = opts.large_mem
+		
+		shared_arg_list.append(output)
+		shared_arg_list.append(threads)
+		shared_arg_list.append(verbose)
+		shared_arg_list.append(do_stdev)
+		shared_arg_list.append(mem)
+		shared_arg_list.append(efficient)
+
+		query_arg_list = []
+		genomes, proteins, hmms = opts.query_genomes, opts.query_proteins, opts.query_hmms
+		#Text file versions of genomes/proteins/hmms
+		gf, pf, hf = opts.qgf, opts.qpf, opts.qhf
+		query_db_name = opts.query_db_name
+		
+		query_arg_list.append(genomes)
+		query_arg_list.append(proteins)
+		query_arg_list.append(hmms)
+		query_arg_list.append(gf)
+		query_arg_list.append(pf)
+		query_arg_list.append(hf)
+		query_arg_list.append(query_db_name)
+		
+		target_arg_list = []
+		genomes, proteins, hmms = opts.target_genomes, opts.target_proteins, opts.target_hmms
+		#Text file versions of genomes/proteins/hmms
+		gf, pf, hf = opts.tgf, opts.tpf, opts.thf
+		target_db_name = opts.target_db_name
+		
+		target_arg_list.append(genomes)
+		target_arg_list.append(proteins)
+		target_arg_list.append(hmms)
+		target_arg_list.append(gf)
+		target_arg_list.append(pf)
+		target_arg_list.append(hf)
+		target_arg_list.append(target_db_name)
+
+		multi_query(query_arg_list, target_arg_list, shared_arg_list)
+		
+	return None
+	
+
+if __name__ == "__main__":
+	main()
+
+