miga-base 1.2.17.0 → 1.2.17.1

Sign up to get free protection for your applications and to get access to all the features.
Files changed (299) hide show
  1. checksums.yaml +4 -4
  2. data/lib/miga/version.rb +1 -1
  3. data/utils/FastAAI/00.Libraries/01.SCG_HMMs/Archaea_SCG.hmm +41964 -0
  4. data/utils/FastAAI/00.Libraries/01.SCG_HMMs/Bacteria_SCG.hmm +32439 -0
  5. data/utils/FastAAI/00.Libraries/01.SCG_HMMs/Complete_SCG_DB.hmm +62056 -0
  6. data/utils/FastAAI/FastAAI +3659 -0
  7. data/utils/FastAAI/FastAAI-legacy/FastAAI +1336 -0
  8. data/utils/FastAAI/FastAAI-legacy/kAAI_v1.0_virus.py +1296 -0
  9. data/utils/FastAAI/README.md +84 -0
  10. data/utils/enveomics/Docs/recplot2.md +244 -0
  11. data/utils/enveomics/Examples/aai-matrix.bash +66 -0
  12. data/utils/enveomics/Examples/ani-matrix.bash +66 -0
  13. data/utils/enveomics/Examples/essential-phylogeny.bash +105 -0
  14. data/utils/enveomics/Examples/unus-genome-phylogeny.bash +100 -0
  15. data/utils/enveomics/LICENSE.txt +73 -0
  16. data/utils/enveomics/Makefile +52 -0
  17. data/utils/enveomics/Manifest/Tasks/aasubs.json +103 -0
  18. data/utils/enveomics/Manifest/Tasks/blasttab.json +790 -0
  19. data/utils/enveomics/Manifest/Tasks/distances.json +161 -0
  20. data/utils/enveomics/Manifest/Tasks/fasta.json +802 -0
  21. data/utils/enveomics/Manifest/Tasks/fastq.json +291 -0
  22. data/utils/enveomics/Manifest/Tasks/graphics.json +126 -0
  23. data/utils/enveomics/Manifest/Tasks/mapping.json +137 -0
  24. data/utils/enveomics/Manifest/Tasks/ogs.json +382 -0
  25. data/utils/enveomics/Manifest/Tasks/other.json +906 -0
  26. data/utils/enveomics/Manifest/Tasks/remote.json +355 -0
  27. data/utils/enveomics/Manifest/Tasks/sequence-identity.json +650 -0
  28. data/utils/enveomics/Manifest/Tasks/tables.json +308 -0
  29. data/utils/enveomics/Manifest/Tasks/trees.json +68 -0
  30. data/utils/enveomics/Manifest/Tasks/variants.json +111 -0
  31. data/utils/enveomics/Manifest/categories.json +165 -0
  32. data/utils/enveomics/Manifest/examples.json +162 -0
  33. data/utils/enveomics/Manifest/tasks.json +4 -0
  34. data/utils/enveomics/Pipelines/assembly.pbs/CONFIG.mock.bash +69 -0
  35. data/utils/enveomics/Pipelines/assembly.pbs/FastA.N50.pl +1 -0
  36. data/utils/enveomics/Pipelines/assembly.pbs/FastA.filterN.pl +1 -0
  37. data/utils/enveomics/Pipelines/assembly.pbs/FastA.length.pl +1 -0
  38. data/utils/enveomics/Pipelines/assembly.pbs/README.md +189 -0
  39. data/utils/enveomics/Pipelines/assembly.pbs/RUNME-2.bash +112 -0
  40. data/utils/enveomics/Pipelines/assembly.pbs/RUNME-3.bash +23 -0
  41. data/utils/enveomics/Pipelines/assembly.pbs/RUNME-4.bash +44 -0
  42. data/utils/enveomics/Pipelines/assembly.pbs/RUNME.bash +50 -0
  43. data/utils/enveomics/Pipelines/assembly.pbs/kSelector.R +37 -0
  44. data/utils/enveomics/Pipelines/assembly.pbs/newbler.pbs +68 -0
  45. data/utils/enveomics/Pipelines/assembly.pbs/newbler_preparator.pl +49 -0
  46. data/utils/enveomics/Pipelines/assembly.pbs/soap.pbs +80 -0
  47. data/utils/enveomics/Pipelines/assembly.pbs/stats.pbs +57 -0
  48. data/utils/enveomics/Pipelines/assembly.pbs/velvet.pbs +63 -0
  49. data/utils/enveomics/Pipelines/blast.pbs/01.pbs.bash +38 -0
  50. data/utils/enveomics/Pipelines/blast.pbs/02.pbs.bash +73 -0
  51. data/utils/enveomics/Pipelines/blast.pbs/03.pbs.bash +21 -0
  52. data/utils/enveomics/Pipelines/blast.pbs/BlastTab.recover_job.pl +72 -0
  53. data/utils/enveomics/Pipelines/blast.pbs/CONFIG.mock.bash +98 -0
  54. data/utils/enveomics/Pipelines/blast.pbs/FastA.split.pl +1 -0
  55. data/utils/enveomics/Pipelines/blast.pbs/README.md +127 -0
  56. data/utils/enveomics/Pipelines/blast.pbs/RUNME.bash +109 -0
  57. data/utils/enveomics/Pipelines/blast.pbs/TASK.check.bash +128 -0
  58. data/utils/enveomics/Pipelines/blast.pbs/TASK.dry.bash +16 -0
  59. data/utils/enveomics/Pipelines/blast.pbs/TASK.eo.bash +22 -0
  60. data/utils/enveomics/Pipelines/blast.pbs/TASK.pause.bash +26 -0
  61. data/utils/enveomics/Pipelines/blast.pbs/TASK.run.bash +89 -0
  62. data/utils/enveomics/Pipelines/blast.pbs/sentinel.pbs.bash +29 -0
  63. data/utils/enveomics/Pipelines/idba.pbs/README.md +49 -0
  64. data/utils/enveomics/Pipelines/idba.pbs/RUNME.bash +95 -0
  65. data/utils/enveomics/Pipelines/idba.pbs/run.pbs +56 -0
  66. data/utils/enveomics/Pipelines/trim.pbs/README.md +54 -0
  67. data/utils/enveomics/Pipelines/trim.pbs/RUNME.bash +70 -0
  68. data/utils/enveomics/Pipelines/trim.pbs/run.pbs +130 -0
  69. data/utils/enveomics/README.md +42 -0
  70. data/utils/enveomics/Scripts/AAsubs.log2ratio.rb +171 -0
  71. data/utils/enveomics/Scripts/Aln.cat.rb +221 -0
  72. data/utils/enveomics/Scripts/Aln.convert.pl +35 -0
  73. data/utils/enveomics/Scripts/AlphaDiversity.pl +152 -0
  74. data/utils/enveomics/Scripts/BedGraph.tad.rb +93 -0
  75. data/utils/enveomics/Scripts/BedGraph.window.rb +71 -0
  76. data/utils/enveomics/Scripts/BlastPairwise.AAsubs.pl +102 -0
  77. data/utils/enveomics/Scripts/BlastTab.addlen.rb +63 -0
  78. data/utils/enveomics/Scripts/BlastTab.advance.bash +48 -0
  79. data/utils/enveomics/Scripts/BlastTab.best_hit_sorted.pl +55 -0
  80. data/utils/enveomics/Scripts/BlastTab.catsbj.pl +104 -0
  81. data/utils/enveomics/Scripts/BlastTab.cogCat.rb +76 -0
  82. data/utils/enveomics/Scripts/BlastTab.filter.pl +47 -0
  83. data/utils/enveomics/Scripts/BlastTab.kegg_pep2path_rest.pl +194 -0
  84. data/utils/enveomics/Scripts/BlastTab.metaxaPrep.pl +104 -0
  85. data/utils/enveomics/Scripts/BlastTab.pairedHits.rb +157 -0
  86. data/utils/enveomics/Scripts/BlastTab.recplot2.R +48 -0
  87. data/utils/enveomics/Scripts/BlastTab.seqdepth.pl +86 -0
  88. data/utils/enveomics/Scripts/BlastTab.seqdepth_ZIP.pl +119 -0
  89. data/utils/enveomics/Scripts/BlastTab.seqdepth_nomedian.pl +86 -0
  90. data/utils/enveomics/Scripts/BlastTab.subsample.pl +47 -0
  91. data/utils/enveomics/Scripts/BlastTab.sumPerHit.pl +114 -0
  92. data/utils/enveomics/Scripts/BlastTab.taxid2taxrank.pl +90 -0
  93. data/utils/enveomics/Scripts/BlastTab.topHits_sorted.rb +123 -0
  94. data/utils/enveomics/Scripts/Chao1.pl +97 -0
  95. data/utils/enveomics/Scripts/CharTable.classify.rb +234 -0
  96. data/utils/enveomics/Scripts/EBIseq2tax.rb +83 -0
  97. data/utils/enveomics/Scripts/FastA.N50.pl +60 -0
  98. data/utils/enveomics/Scripts/FastA.extract.rb +152 -0
  99. data/utils/enveomics/Scripts/FastA.filter.pl +52 -0
  100. data/utils/enveomics/Scripts/FastA.filterLen.pl +28 -0
  101. data/utils/enveomics/Scripts/FastA.filterN.pl +60 -0
  102. data/utils/enveomics/Scripts/FastA.fragment.rb +100 -0
  103. data/utils/enveomics/Scripts/FastA.gc.pl +42 -0
  104. data/utils/enveomics/Scripts/FastA.interpose.pl +93 -0
  105. data/utils/enveomics/Scripts/FastA.length.pl +38 -0
  106. data/utils/enveomics/Scripts/FastA.mask.rb +89 -0
  107. data/utils/enveomics/Scripts/FastA.per_file.pl +36 -0
  108. data/utils/enveomics/Scripts/FastA.qlen.pl +57 -0
  109. data/utils/enveomics/Scripts/FastA.rename.pl +65 -0
  110. data/utils/enveomics/Scripts/FastA.revcom.pl +23 -0
  111. data/utils/enveomics/Scripts/FastA.sample.rb +98 -0
  112. data/utils/enveomics/Scripts/FastA.slider.pl +85 -0
  113. data/utils/enveomics/Scripts/FastA.split.pl +55 -0
  114. data/utils/enveomics/Scripts/FastA.split.rb +79 -0
  115. data/utils/enveomics/Scripts/FastA.subsample.pl +131 -0
  116. data/utils/enveomics/Scripts/FastA.tag.rb +65 -0
  117. data/utils/enveomics/Scripts/FastA.toFastQ.rb +69 -0
  118. data/utils/enveomics/Scripts/FastA.wrap.rb +48 -0
  119. data/utils/enveomics/Scripts/FastQ.filter.pl +54 -0
  120. data/utils/enveomics/Scripts/FastQ.interpose.pl +90 -0
  121. data/utils/enveomics/Scripts/FastQ.maskQual.rb +89 -0
  122. data/utils/enveomics/Scripts/FastQ.offset.pl +90 -0
  123. data/utils/enveomics/Scripts/FastQ.split.pl +53 -0
  124. data/utils/enveomics/Scripts/FastQ.tag.rb +70 -0
  125. data/utils/enveomics/Scripts/FastQ.test-error.rb +81 -0
  126. data/utils/enveomics/Scripts/FastQ.toFastA.awk +24 -0
  127. data/utils/enveomics/Scripts/GFF.catsbj.pl +127 -0
  128. data/utils/enveomics/Scripts/GenBank.add_fields.rb +84 -0
  129. data/utils/enveomics/Scripts/HMM.essential.rb +351 -0
  130. data/utils/enveomics/Scripts/HMM.haai.rb +168 -0
  131. data/utils/enveomics/Scripts/HMMsearch.extractIds.rb +83 -0
  132. data/utils/enveomics/Scripts/JPlace.distances.rb +88 -0
  133. data/utils/enveomics/Scripts/JPlace.to_iToL.rb +320 -0
  134. data/utils/enveomics/Scripts/M5nr.getSequences.rb +81 -0
  135. data/utils/enveomics/Scripts/MeTaxa.distribution.pl +198 -0
  136. data/utils/enveomics/Scripts/MyTaxa.fragsByTax.pl +35 -0
  137. data/utils/enveomics/Scripts/MyTaxa.seq-taxrank.rb +49 -0
  138. data/utils/enveomics/Scripts/NCBIacc2tax.rb +92 -0
  139. data/utils/enveomics/Scripts/Newick.autoprune.R +27 -0
  140. data/utils/enveomics/Scripts/RAxML-EPA.to_iToL.pl +228 -0
  141. data/utils/enveomics/Scripts/RecPlot2.compareIdentities.R +32 -0
  142. data/utils/enveomics/Scripts/RefSeq.download.bash +48 -0
  143. data/utils/enveomics/Scripts/SRA.download.bash +55 -0
  144. data/utils/enveomics/Scripts/TRIBS.plot-test.R +36 -0
  145. data/utils/enveomics/Scripts/TRIBS.test.R +39 -0
  146. data/utils/enveomics/Scripts/Table.barplot.R +31 -0
  147. data/utils/enveomics/Scripts/Table.df2dist.R +30 -0
  148. data/utils/enveomics/Scripts/Table.filter.pl +61 -0
  149. data/utils/enveomics/Scripts/Table.merge.pl +77 -0
  150. data/utils/enveomics/Scripts/Table.prefScore.R +60 -0
  151. data/utils/enveomics/Scripts/Table.replace.rb +69 -0
  152. data/utils/enveomics/Scripts/Table.round.rb +63 -0
  153. data/utils/enveomics/Scripts/Table.split.pl +57 -0
  154. data/utils/enveomics/Scripts/Taxonomy.silva2ncbi.rb +227 -0
  155. data/utils/enveomics/Scripts/VCF.KaKs.rb +147 -0
  156. data/utils/enveomics/Scripts/VCF.SNPs.rb +88 -0
  157. data/utils/enveomics/Scripts/aai.rb +421 -0
  158. data/utils/enveomics/Scripts/ani.rb +362 -0
  159. data/utils/enveomics/Scripts/anir.rb +137 -0
  160. data/utils/enveomics/Scripts/clust.rand.rb +102 -0
  161. data/utils/enveomics/Scripts/gi2tax.rb +103 -0
  162. data/utils/enveomics/Scripts/in_silico_GA_GI.pl +96 -0
  163. data/utils/enveomics/Scripts/lib/data/dupont_2012_essential.hmm.gz +0 -0
  164. data/utils/enveomics/Scripts/lib/data/lee_2019_essential.hmm.gz +0 -0
  165. data/utils/enveomics/Scripts/lib/enveomics.R +1 -0
  166. data/utils/enveomics/Scripts/lib/enveomics_rb/anir.rb +293 -0
  167. data/utils/enveomics/Scripts/lib/enveomics_rb/bm_set.rb +175 -0
  168. data/utils/enveomics/Scripts/lib/enveomics_rb/enveomics.rb +24 -0
  169. data/utils/enveomics/Scripts/lib/enveomics_rb/errors.rb +17 -0
  170. data/utils/enveomics/Scripts/lib/enveomics_rb/gmm_em.rb +30 -0
  171. data/utils/enveomics/Scripts/lib/enveomics_rb/jplace.rb +253 -0
  172. data/utils/enveomics/Scripts/lib/enveomics_rb/match.rb +88 -0
  173. data/utils/enveomics/Scripts/lib/enveomics_rb/og.rb +182 -0
  174. data/utils/enveomics/Scripts/lib/enveomics_rb/rbm.rb +49 -0
  175. data/utils/enveomics/Scripts/lib/enveomics_rb/remote_data.rb +74 -0
  176. data/utils/enveomics/Scripts/lib/enveomics_rb/seq_range.rb +237 -0
  177. data/utils/enveomics/Scripts/lib/enveomics_rb/stats/rand.rb +31 -0
  178. data/utils/enveomics/Scripts/lib/enveomics_rb/stats/sample.rb +152 -0
  179. data/utils/enveomics/Scripts/lib/enveomics_rb/stats.rb +3 -0
  180. data/utils/enveomics/Scripts/lib/enveomics_rb/utils.rb +74 -0
  181. data/utils/enveomics/Scripts/lib/enveomics_rb/vcf.rb +135 -0
  182. data/utils/enveomics/Scripts/ogs.annotate.rb +88 -0
  183. data/utils/enveomics/Scripts/ogs.core-pan.rb +160 -0
  184. data/utils/enveomics/Scripts/ogs.extract.rb +125 -0
  185. data/utils/enveomics/Scripts/ogs.mcl.rb +186 -0
  186. data/utils/enveomics/Scripts/ogs.rb +104 -0
  187. data/utils/enveomics/Scripts/ogs.stats.rb +131 -0
  188. data/utils/enveomics/Scripts/rbm-legacy.rb +172 -0
  189. data/utils/enveomics/Scripts/rbm.rb +108 -0
  190. data/utils/enveomics/Scripts/sam.filter.rb +148 -0
  191. data/utils/enveomics/Tests/Makefile +10 -0
  192. data/utils/enveomics/Tests/Mgen_M2288.faa +3189 -0
  193. data/utils/enveomics/Tests/Mgen_M2288.fna +8282 -0
  194. data/utils/enveomics/Tests/Mgen_M2321.fna +8288 -0
  195. data/utils/enveomics/Tests/Nequ_Kin4M.faa +2970 -0
  196. data/utils/enveomics/Tests/Xanthomonas_oryzae-PilA.tribs.Rdata +0 -0
  197. data/utils/enveomics/Tests/Xanthomonas_oryzae-PilA.txt +7 -0
  198. data/utils/enveomics/Tests/Xanthomonas_oryzae.aai-mat.tsv +17 -0
  199. data/utils/enveomics/Tests/Xanthomonas_oryzae.aai.tsv +137 -0
  200. data/utils/enveomics/Tests/a_mg.cds-go.blast.tsv +123 -0
  201. data/utils/enveomics/Tests/a_mg.reads-cds.blast.tsv +200 -0
  202. data/utils/enveomics/Tests/a_mg.reads-cds.counts.tsv +55 -0
  203. data/utils/enveomics/Tests/alkB.nwk +1 -0
  204. data/utils/enveomics/Tests/anthrax-cansnp-data.tsv +13 -0
  205. data/utils/enveomics/Tests/anthrax-cansnp-key.tsv +17 -0
  206. data/utils/enveomics/Tests/hiv1.faa +59 -0
  207. data/utils/enveomics/Tests/hiv1.fna +134 -0
  208. data/utils/enveomics/Tests/hiv2.faa +70 -0
  209. data/utils/enveomics/Tests/hiv_mix-hiv1.blast.tsv +233 -0
  210. data/utils/enveomics/Tests/hiv_mix-hiv1.blast.tsv.lim +1 -0
  211. data/utils/enveomics/Tests/hiv_mix-hiv1.blast.tsv.rec +233 -0
  212. data/utils/enveomics/Tests/phyla_counts.tsv +10 -0
  213. data/utils/enveomics/Tests/primate_lentivirus.ogs +11 -0
  214. data/utils/enveomics/Tests/primate_lentivirus.rbm/hiv1-hiv1.rbm +9 -0
  215. data/utils/enveomics/Tests/primate_lentivirus.rbm/hiv1-hiv2.rbm +8 -0
  216. data/utils/enveomics/Tests/primate_lentivirus.rbm/hiv1-siv.rbm +6 -0
  217. data/utils/enveomics/Tests/primate_lentivirus.rbm/hiv2-hiv2.rbm +9 -0
  218. data/utils/enveomics/Tests/primate_lentivirus.rbm/hiv2-siv.rbm +6 -0
  219. data/utils/enveomics/Tests/primate_lentivirus.rbm/siv-siv.rbm +6 -0
  220. data/utils/enveomics/build_enveomics_r.bash +45 -0
  221. data/utils/enveomics/enveomics.R/DESCRIPTION +31 -0
  222. data/utils/enveomics/enveomics.R/NAMESPACE +39 -0
  223. data/utils/enveomics/enveomics.R/R/autoprune.R +155 -0
  224. data/utils/enveomics/enveomics.R/R/barplot.R +184 -0
  225. data/utils/enveomics/enveomics.R/R/cliopts.R +135 -0
  226. data/utils/enveomics/enveomics.R/R/df2dist.R +154 -0
  227. data/utils/enveomics/enveomics.R/R/growthcurve.R +331 -0
  228. data/utils/enveomics/enveomics.R/R/prefscore.R +79 -0
  229. data/utils/enveomics/enveomics.R/R/recplot.R +354 -0
  230. data/utils/enveomics/enveomics.R/R/recplot2.R +1631 -0
  231. data/utils/enveomics/enveomics.R/R/tribs.R +583 -0
  232. data/utils/enveomics/enveomics.R/R/utils.R +80 -0
  233. data/utils/enveomics/enveomics.R/README.md +81 -0
  234. data/utils/enveomics/enveomics.R/data/growth.curves.rda +0 -0
  235. data/utils/enveomics/enveomics.R/data/phyla.counts.rda +0 -0
  236. data/utils/enveomics/enveomics.R/man/cash-enve.GrowthCurve-method.Rd +16 -0
  237. data/utils/enveomics/enveomics.R/man/cash-enve.RecPlot2-method.Rd +16 -0
  238. data/utils/enveomics/enveomics.R/man/cash-enve.RecPlot2.Peak-method.Rd +16 -0
  239. data/utils/enveomics/enveomics.R/man/enve.GrowthCurve-class.Rd +25 -0
  240. data/utils/enveomics/enveomics.R/man/enve.TRIBS-class.Rd +46 -0
  241. data/utils/enveomics/enveomics.R/man/enve.TRIBS.merge.Rd +23 -0
  242. data/utils/enveomics/enveomics.R/man/enve.TRIBStest-class.Rd +47 -0
  243. data/utils/enveomics/enveomics.R/man/enve.__prune.iter.Rd +23 -0
  244. data/utils/enveomics/enveomics.R/man/enve.__prune.reduce.Rd +23 -0
  245. data/utils/enveomics/enveomics.R/man/enve.__tribs.Rd +40 -0
  246. data/utils/enveomics/enveomics.R/man/enve.barplot.Rd +103 -0
  247. data/utils/enveomics/enveomics.R/man/enve.cliopts.Rd +67 -0
  248. data/utils/enveomics/enveomics.R/man/enve.col.alpha.Rd +24 -0
  249. data/utils/enveomics/enveomics.R/man/enve.col2alpha.Rd +19 -0
  250. data/utils/enveomics/enveomics.R/man/enve.df2dist.Rd +45 -0
  251. data/utils/enveomics/enveomics.R/man/enve.df2dist.group.Rd +44 -0
  252. data/utils/enveomics/enveomics.R/man/enve.df2dist.list.Rd +47 -0
  253. data/utils/enveomics/enveomics.R/man/enve.growthcurve.Rd +75 -0
  254. data/utils/enveomics/enveomics.R/man/enve.prefscore.Rd +50 -0
  255. data/utils/enveomics/enveomics.R/man/enve.prune.dist.Rd +44 -0
  256. data/utils/enveomics/enveomics.R/man/enve.recplot.Rd +139 -0
  257. data/utils/enveomics/enveomics.R/man/enve.recplot2-class.Rd +45 -0
  258. data/utils/enveomics/enveomics.R/man/enve.recplot2.ANIr.Rd +24 -0
  259. data/utils/enveomics/enveomics.R/man/enve.recplot2.Rd +77 -0
  260. data/utils/enveomics/enveomics.R/man/enve.recplot2.__counts.Rd +25 -0
  261. data/utils/enveomics/enveomics.R/man/enve.recplot2.__peakHist.Rd +21 -0
  262. data/utils/enveomics/enveomics.R/man/enve.recplot2.__whichClosestPeak.Rd +19 -0
  263. data/utils/enveomics/enveomics.R/man/enve.recplot2.changeCutoff.Rd +19 -0
  264. data/utils/enveomics/enveomics.R/man/enve.recplot2.compareIdentities.Rd +47 -0
  265. data/utils/enveomics/enveomics.R/man/enve.recplot2.coordinates.Rd +29 -0
  266. data/utils/enveomics/enveomics.R/man/enve.recplot2.corePeak.Rd +18 -0
  267. data/utils/enveomics/enveomics.R/man/enve.recplot2.extractWindows.Rd +45 -0
  268. data/utils/enveomics/enveomics.R/man/enve.recplot2.findPeaks.Rd +36 -0
  269. data/utils/enveomics/enveomics.R/man/enve.recplot2.findPeaks.__em_e.Rd +19 -0
  270. data/utils/enveomics/enveomics.R/man/enve.recplot2.findPeaks.__em_m.Rd +19 -0
  271. data/utils/enveomics/enveomics.R/man/enve.recplot2.findPeaks.__emauto_one.Rd +27 -0
  272. data/utils/enveomics/enveomics.R/man/enve.recplot2.findPeaks.__mow_one.Rd +52 -0
  273. data/utils/enveomics/enveomics.R/man/enve.recplot2.findPeaks.__mower.Rd +17 -0
  274. data/utils/enveomics/enveomics.R/man/enve.recplot2.findPeaks.em.Rd +51 -0
  275. data/utils/enveomics/enveomics.R/man/enve.recplot2.findPeaks.emauto.Rd +43 -0
  276. data/utils/enveomics/enveomics.R/man/enve.recplot2.findPeaks.mower.Rd +82 -0
  277. data/utils/enveomics/enveomics.R/man/enve.recplot2.peak-class.Rd +59 -0
  278. data/utils/enveomics/enveomics.R/man/enve.recplot2.seqdepth.Rd +27 -0
  279. data/utils/enveomics/enveomics.R/man/enve.recplot2.windowDepthThreshold.Rd +36 -0
  280. data/utils/enveomics/enveomics.R/man/enve.selvector.Rd +23 -0
  281. data/utils/enveomics/enveomics.R/man/enve.tribs.Rd +68 -0
  282. data/utils/enveomics/enveomics.R/man/enve.tribs.test.Rd +28 -0
  283. data/utils/enveomics/enveomics.R/man/enve.truncate.Rd +27 -0
  284. data/utils/enveomics/enveomics.R/man/growth.curves.Rd +14 -0
  285. data/utils/enveomics/enveomics.R/man/phyla.counts.Rd +13 -0
  286. data/utils/enveomics/enveomics.R/man/plot.enve.GrowthCurve.Rd +78 -0
  287. data/utils/enveomics/enveomics.R/man/plot.enve.TRIBS.Rd +46 -0
  288. data/utils/enveomics/enveomics.R/man/plot.enve.TRIBStest.Rd +45 -0
  289. data/utils/enveomics/enveomics.R/man/plot.enve.recplot2.Rd +125 -0
  290. data/utils/enveomics/enveomics.R/man/summary.enve.GrowthCurve.Rd +19 -0
  291. data/utils/enveomics/enveomics.R/man/summary.enve.TRIBS.Rd +19 -0
  292. data/utils/enveomics/enveomics.R/man/summary.enve.TRIBStest.Rd +19 -0
  293. data/utils/enveomics/globals.mk +8 -0
  294. data/utils/enveomics/manifest.json +9 -0
  295. data/utils/multitrim/Multitrim How-To.pdf +0 -0
  296. data/utils/multitrim/README.md +67 -0
  297. data/utils/multitrim/multitrim.py +1555 -0
  298. data/utils/multitrim/multitrim.yml +13 -0
  299. metadata +301 -5
@@ -0,0 +1,906 @@
1
+ {
2
+ "tasks": [
3
+ {
4
+ "task": "Aln.cat.rb",
5
+ "description": ["Concatenates several multiple alignments in FastA",
6
+ "format into a single multiple alignment. The IDs of the sequences",
7
+ "(or the ID prefixes, if using --ignore-after) must coincide across",
8
+ "files."],
9
+ "help_arg": "--help",
10
+ "options": [
11
+ {
12
+ "opt": "--coords",
13
+ "arg": "out_file",
14
+ "description": "Output file of coordinates in RAxML-compliant format."
15
+ },
16
+ {
17
+ "opt": "--ignore-after",
18
+ "arg": "string",
19
+ "description": ["Remove everything in the IDs after the specified",
20
+ "string."]
21
+ },
22
+ {
23
+ "opt": "--remove-invariable",
24
+ "description": "Remove invariable sites.",
25
+ "note": ["Invariable sites are defined as columns with only one",
26
+ "state and undefined characters. Additional ambiguous characters",
27
+ "may exist and should be declared using --undefined."]
28
+ },
29
+ {
30
+ "opt": "--missing-char",
31
+ "description": "Character denoting missing data.",
32
+ "arg": "character",
33
+ "default": "-"
34
+ },
35
+ {
36
+ "opt": "--model",
37
+ "description": "Name of the model to use if --coords is used.",
38
+ "note": "See RAxML's documentation for additional information.",
39
+ "arg": "string",
40
+ "default": "AUTO"
41
+ },
42
+ {
43
+ "opt": "--undefined",
44
+ "arg": "string",
45
+ "description": ["All characters to be regarded as 'undefined'. It",
46
+ "should include all ambiguous and missing data chars. Ignored",
47
+ "unless --remove-invariable."],
48
+ "default": "-.Xx?"
49
+ },
50
+ {
51
+ "opt": "--quiet",
52
+ "description": "Run quietly (no STDERR output)."
53
+ },
54
+ {
55
+ "name": "Input alignments",
56
+ "arg": "in_file",
57
+ "multiple_sep": " ",
58
+ "mandatory": true,
59
+ "description": "Input alignments in FastA format."
60
+ },
61
+ ">",
62
+ {
63
+ "name": "Output alignment",
64
+ "arg": "out_file",
65
+ "mandatory": true,
66
+ "description": "Output concatenated alignment."
67
+ }
68
+ ]
69
+ },
70
+ {
71
+ "task": "Aln.convert.pl",
72
+ "description": "Translates between different alignment formats.",
73
+ "help_arg": "",
74
+ "requires": [
75
+ {
76
+ "perl_lib": "Bio::AlignIO"
77
+ }
78
+ ],
79
+ "cite": [["Stajich et al, 2002, GRes",
80
+ "http://dx.doi.org/10.1101/gr.361602"]],
81
+ "options": [
82
+ {
83
+ "name": "In-format",
84
+ "description": "Input file's format.",
85
+ "mandatory": true,
86
+ "arg": "select",
87
+ "values": ["bl2seq", "clustalw", "emboss", "fasta", "maf", "mase",
88
+ "mega", "meme", "metafasta", "msf", "nexus", "pfam", "phylip", "po",
89
+ "prodom", "psi", "selex", "stockholm", "XMFA", "arp"]
90
+ },
91
+ {
92
+ "name": "Out-format",
93
+ "description": "Output file's format.",
94
+ "mandatory": true,
95
+ "arg": "select",
96
+ "values": ["bl2seq", "clustalw", "emboss", "fasta", "maf", "mase",
97
+ "mega", "meme", "metafasta", "msf", "nexus", "pfam", "phylip", "po",
98
+ "prodom", "psi", "selex", "stockholm", "XMFA", "arp"]
99
+ },
100
+ "<",
101
+ {
102
+ "arg": "in_file",
103
+ "mandatory": true
104
+ },
105
+ ">",
106
+ {
107
+ "arg": "out_file",
108
+ "mandatory": true
109
+ }
110
+ ]
111
+ },
112
+ {
113
+ "task": "AlphaDiversity.pl",
114
+ "description": ["Takes a table of OTU abundance in one or more samples",
115
+ "and calculates the Rao (Q_alpha), Rao-Jost (Q_alpha_eqv), Shannon",
116
+ "(Hprime), and inverse Simpson (1_lambda) indices of alpha diversity",
117
+ "for each sample."],
118
+ "help_arg": "--help",
119
+ "see_also": ["Chao1.pl"],
120
+ "options": [
121
+ {
122
+ "name": "Input table",
123
+ "opt": "-i",
124
+ "arg": "in_file",
125
+ "description": ["Columns: samples, rows: OTUs, first column: OTU",
126
+ "names)."],
127
+ "mandatory": true
128
+ },
129
+ {
130
+ "name": "Discard rows",
131
+ "opt": "-r",
132
+ "arg": "integer",
133
+ "description": "Number of rows to ignore.",
134
+ "default": 0
135
+ },
136
+ {
137
+ "name": "Discard left columns",
138
+ "opt": "-c",
139
+ "arg": "integer",
140
+ "description": ["Number of columns to ignore after the first column",
141
+ "(i.e., between the first column, containing the name of the",
142
+ "categories, and the first column of abundance values)."],
143
+ "default": 0
144
+ },
145
+ {
146
+ "name": "Discard right columns",
147
+ "opt": "-C",
148
+ "arg": "integer",
149
+ "description": "Number of columns to ignore at the end of each line.",
150
+ "default": 0
151
+ },
152
+ {
153
+ "name": "Delimiter",
154
+ "opt": "-d",
155
+ "arg": "string",
156
+ "description": "Character (or string) separating columns.",
157
+ "note": ["Supported escaped characters are: '\\t' (tabulation), and",
158
+ "'\\0' (null bit)."],
159
+ "default": "\\t"
160
+ },
161
+ {
162
+ "name": "Headers",
163
+ "opt": "-h",
164
+ "description": ["The first row is assumed to have the names of the",
165
+ "samples."]
166
+ },
167
+ {
168
+ "name": "Distances file",
169
+ "opt": "-D",
170
+ "arg": "in_file",
171
+ "description": ["A squared matrix (or bottom-left half matrix) with",
172
+ "the distances between categories (OTUs or functions). The first",
173
+ "column must contain the names of the categories, and it shouldn't",
174
+ "have headers. If not set, all distances are assumed to be one."],
175
+ "note": "Only used for Rao."
176
+ },
177
+ {
178
+ "name": "Skip Rao",
179
+ "opt": "-R",
180
+ "description": "Do not calculate Rao indices.",
181
+ "note": ["This significantly decreases the total running time. Note",
182
+ "that Rao indices are highly susceptible to precision errors, and",
183
+ "shouldn't be trusted for very big numbers."]
184
+ },
185
+ {
186
+ "name": "q for qD",
187
+ "opt": "-q",
188
+ "arg": "integer",
189
+ "description": "Estimate the qD index (true diversity order q).",
190
+ "default": 0
191
+ },
192
+ ">",
193
+ {
194
+ "arg": "out_file",
195
+ "mandatory": true
196
+ }
197
+ ]
198
+ },
199
+ {
200
+ "task": "Chao1.pl",
201
+ "description": ["Takes a table of OTU abundance in one or more samples",
202
+ "and calculates the chao1 index (with 95% Confidence Interval) for",
203
+ "each sample. To use it with Qiime OTU Tables, run it ignoring 1",
204
+ "left column and with header."],
205
+ "cite": [["Chao, 1984, Scandinavian J Stat",
206
+ "http://www.jstor.org/stable/4615964"]],
207
+ "see_also": ["AlphaDiversity.pl"],
208
+ "help_arg": "--help",
209
+ "options": [
210
+ {
211
+ "name": "Input file",
212
+ "opt": "-i",
213
+ "arg": "in_file",
214
+ "mandatory": true,
215
+ "description": "Input table (columns:samples, rows:OTUs)."
216
+ },
217
+ {
218
+ "name": "Rows",
219
+ "opt": "-r",
220
+ "arg": "integer",
221
+ "default": 0,
222
+ "description": "Number of rows to ignore."
223
+ },
224
+ {
225
+ "name": "Left columns to ignore",
226
+ "opt": "-c",
227
+ "arg": "integer",
228
+ "default": 0,
229
+ "description": "Number of columns to ignore."
230
+ },
231
+ {
232
+ "name": "Right columns to ignore",
233
+ "opt": "-C",
234
+ "arg": "integer",
235
+ "default": 0,
236
+ "description": "Number of columns to ignore at the end."
237
+ },
238
+ {
239
+ "name": "Delimiter",
240
+ "opt": "-d",
241
+ "arg": "string",
242
+ "default": "\\t",
243
+ "description": "Delimiter between columns.",
244
+ "note": ["Supported escaped characters are: \\t (tabulation),",
245
+ "and \\0 (null bit)."]
246
+ },
247
+ {
248
+ "name": "Header",
249
+ "opt": "-h",
250
+ "description": ["If set, the first row is assumed to have the names",
251
+ "of the samples."]
252
+ },
253
+ ">",
254
+ {
255
+ "arg": "out_file",
256
+ "mandatory": true,
257
+ "description": ["Chao1 index and 95% CI bounds for each sample, in",
258
+ "tab-delimited format."]
259
+ }
260
+ ]
261
+ },
262
+ {
263
+ "task": "CharTable.classify.rb",
264
+ "description": ["Uses a dichotomous key to classify objects parsing a",
265
+ "character table."],
266
+ "help_arg": "--help",
267
+ "options": [
268
+ {
269
+ "opt": "--table",
270
+ "arg": "in_file",
271
+ "mandatory": true,
272
+ "description": ["Input table containing the states (columns) per",
273
+ "object (row). It must be tab-delimited and with row and column",
274
+ "names."]
275
+ },
276
+ {
277
+ "opt": "--key",
278
+ "arg": "in_file",
279
+ "mandatory": true,
280
+ "description": ["Input table containing the dychotomous key in",
281
+ "linked style, defined in four columns (can contain #-lead comment",
282
+ "lines): (1) ID of the step, typically a sequential integer. (2)",
283
+ "Name of character to evaluate. Must coincide with the -t headers.",
284
+ "(3) First character decision (see below). (4) Second character",
285
+ "decision (see below).\nA character decision must be formated as:",
286
+ "state (must coincide with the values in -t), colon (:), step to",
287
+ "follow. If the state is * (star) any state triggers the decision",
288
+ "(this should be the norm in column 4). The step to follow should",
289
+ "be a step ID in square brackets, or the name of the",
290
+ "classification."]
291
+ },
292
+ {
293
+ "opt": "--classification",
294
+ "arg": "out_file",
295
+ "description": ["Two-column table with the classification of the",
296
+ "input objects."]
297
+ },
298
+ {
299
+ "opt": "--newick",
300
+ "arg": "out_file",
301
+ "description": ["Tree containing all the classified objects. This",
302
+ "only makes sense for synoptic keys."]
303
+ },
304
+ {
305
+ "opt": "--quiet",
306
+ "description": "Run quietly (no STDERR output)."
307
+ }
308
+ ]
309
+ },
310
+ {
311
+ "task": "GenBank.add_fields.rb",
312
+ "description": "Adds annotations to GenBank files.",
313
+ "help_arg": "--help",
314
+ "options": [
315
+ {
316
+ "opt": "--genbank",
317
+ "arg": "in_file",
318
+ "mandatory": true,
319
+ "description": "Input GenBank file."
320
+ },
321
+ {
322
+ "opt": "--table",
323
+ "arg": "in_file",
324
+ "mandatory": true,
325
+ "description": ["Input file containing the annotations. It must be a",
326
+ "tab-delimited raw table including a header row with the names of",
327
+ "the fields."]
328
+ },
329
+ {
330
+ "opt": "--out",
331
+ "arg": "out_file",
332
+ "mandatory": true,
333
+ "description": "Output file containing the annotated GenBank."
334
+ },
335
+ {
336
+ "opt": "--key",
337
+ "arg": "integer",
338
+ "default": 1,
339
+ "description": "Key of the column to use as identifier."
340
+ },
341
+ {
342
+ "opt": "--split",
343
+ "arg": "string",
344
+ "default": "#",
345
+ "description": ["String that separates multiple entries in the",
346
+ "annotation features."]
347
+ },
348
+ {
349
+ "opt": "--quiet",
350
+ "description": "Run quietly (no STDERR output)."
351
+ }
352
+ ]
353
+ },
354
+ {
355
+ "task": "HMM.essential.rb",
356
+ "description": ["Finds and extracts a collection of essential proteins",
357
+ "suitable for genome completeness evaluation and phylogenetic",
358
+ "analyses in Archaea and Bacteria."],
359
+ "warn": ["Most complete bacterial genomes contain only 106/111 genes in",
360
+ "this collection, therefore producing a completeness of 95.5%, and",
361
+ "most archaeal genomes only contain 26/111 genes, producing a",
362
+ "completeness of 23.4%. Use the options --bacteria and/or --archaea to",
363
+ "ignore models often missing in one or both domains. Note that even",
364
+ "with these options, some complete archaeal genomes result in very low",
365
+ "values of completeness (e.g., Nanoarchaeum equitans returns 88.5%)."],
366
+ "help_arg": "--help",
367
+ "requires": [
368
+ {
369
+ "description": "HMMer 3.0+",
370
+ "test": "hmmsearch -h",
371
+ "source_url": "http://hmmer.janelia.org/software"
372
+ }
373
+ ],
374
+ "cite": [
375
+ ["Eddy, 2011, PLoS CB",
376
+ "http://dx.doi.org/10.1371/journal.pcbi.1002195"],
377
+ ["Dupont et al, 2012, ISME J",
378
+ "https://doi.org/10.1038/ismej.2011.189"],
379
+ ["Rodriguez-R et al, 2014, ISME J",
380
+ "https://doi.org/10.1038/ismej.2015.5"],
381
+ ["Lee, 2019, Bioinf",
382
+ "https://doi.org/10.1093/bioinformatics/btz188"],
383
+ ["Eren et al, 2015, PeerJ",
384
+ "https://doi.org/10.7717/peerj.1319"]
385
+ ],
386
+ "options": [
387
+ {
388
+ "name": "Input file",
389
+ "opt": "--in",
390
+ "arg": "in_file",
391
+ "mandatory": true,
392
+ "description": "FastA file containing all the proteins in the genome."
393
+ },
394
+ {
395
+ "opt": "--collection",
396
+ "arg": "string",
397
+ "default": "dupont_2012",
398
+ "description": ["Reference collection of essential proteins to use.",
399
+ "One of: dupont_2012 (default, Dupont et al 2012 modified by",
400
+ "Rodriguez-R et al 2015), or lee_2019 (Lee 2019 modified by Eren",
401
+ "et al 2015)."]
402
+ },
403
+ {
404
+ "name": "Output file",
405
+ "opt": "--out",
406
+ "arg": "out_file",
407
+ "description": ["FastA file with the translated essential genes. By",
408
+ "default the file is not produced."]
409
+ },
410
+ {
411
+ "opt": "--per-model",
412
+ "arg": "out_file",
413
+ "description": ["Prefix of translated genes in independent files",
414
+ "with the name of the model appended. By default files are not",
415
+ "produced."]
416
+ },
417
+ {
418
+ "opt": "--report",
419
+ "arg": "out_file",
420
+ "description": ["Path to the report file. By default, the report is",
421
+ "sent to the STDOUT."]
422
+ },
423
+ {
424
+ "name": "HMMsearch output",
425
+ "opt": "--hmm-out",
426
+ "arg": "out_file",
427
+ "description": ["Save HMMsearch output in this file. By default,",
428
+ "not saved."]
429
+ },
430
+ {
431
+ "opt": "--alignments",
432
+ "opt": "out_file",
433
+ "description": ["Save the aligned proteins in this file. By default,",
434
+ "not saved."]
435
+ },
436
+ {
437
+ "opt": "--bacteria",
438
+ "description": "If set, ignores models typically missing in Bacteria."
439
+ },
440
+ {
441
+ "opt": "--archaea",
442
+ "description": "If set, ignores models typically missing in Archaea."
443
+ },
444
+ {
445
+ "opt": "--genome-eq",
446
+ "description": ["If set, ignores models not suitable for",
447
+ "genome-equivalents estimations. See Rodriguez-R et al, 2015, ISME",
448
+ "J 9(9):1928-1940."],
449
+ "source_url": "http://www.nature.com/ismej/journal/vaop/ncurrent/full/ismej20155a.html"
450
+ },
451
+ {
452
+ "opt": "--rename",
453
+ "arg": "string",
454
+ "description": ["If set, renames the sequences with the string",
455
+ "provided and appends it with pipe (|) and the gene name (except",
456
+ "in --per-model files)."]
457
+ },
458
+ {
459
+ "opt": "--no-stats",
460
+ "description": ["If set, no statistics are reported on genome",
461
+ "evaluation."]
462
+ },
463
+ {
464
+ "opt": "--no-genes",
465
+ "description": ["If set, statistics won't include the lists of",
466
+ "missing/multi-copy genes."]
467
+ },
468
+ {
469
+ "opt": "--metagenome",
470
+ "description": ["If set, it allows for multiple copies of each gene",
471
+ "and turns on metagenomic report mode."]
472
+ },
473
+ {
474
+ "opt": "--list-models",
475
+ "description": ["If set, it only lists the models and exits.",
476
+ "Compatible with 'Archaea', 'Bacteria', 'Genome eq', and 'Quiet';",
477
+ "ignores all other parameters."]
478
+ },
479
+ {
480
+ "opt": "--bin",
481
+ "arg": "in_dir",
482
+ "description": "Directory containing the binaries of HMMer 3.0+."
483
+ },
484
+ {
485
+ "opt": "--model-file",
486
+ "arg": "in_file",
487
+ "description": "External file containing models to search."
488
+ },
489
+ {
490
+ "opt": "--threads",
491
+ "arg": "integer",
492
+ "default": 2,
493
+ "description": "Number of parallel threads to be used."
494
+ },
495
+ {
496
+ "opt": "--quiet",
497
+ "description": "Run quietly (no STDERR output)."
498
+ }
499
+ ]
500
+ },
501
+ {
502
+ "task": "HMMsearch.extractIds.rb",
503
+ "description": ["Extracts the sequence IDs and query model form a",
504
+ "(multiple) HMMsearch report (for HMMer 3.0)."],
505
+ "help_arg": "--help",
506
+ "options": [
507
+ {
508
+ "opt": "--all-evalue",
509
+ "arg": "float",
510
+ "description": "Maximum e-value of sequence to report result."
511
+ },
512
+ {
513
+ "opt": "--all-score",
514
+ "arg": "float",
515
+ "description": "Minimum score of sequence to report result."
516
+ },
517
+ {
518
+ "opt": "--best-evalue",
519
+ "arg": "float",
520
+ "description": "Maximum e-value of best domain to report result."
521
+ },
522
+ {
523
+ "opt": "--best-score",
524
+ "arg": "float",
525
+ "description": "Minimum score of best domain to report result."
526
+ },
527
+ {
528
+ "opt": "--no-model",
529
+ "description": "Do not include the query model in the output list."
530
+ },
531
+ {
532
+ "opt": "--quiet",
533
+ "description": "Run quietly."
534
+ },
535
+ "<",
536
+ {
537
+ "arg": "in_file",
538
+ "mandatory": true,
539
+ "description": "Input HMMsearch text file."
540
+ },
541
+ ">",
542
+ {
543
+ "arg": "out_file",
544
+ "mandatory": true,
545
+ "description": "Output list of IDs."
546
+ }
547
+ ]
548
+ },
549
+ {
550
+ "task": "JPlace.distances.rb",
551
+ "description": ["Extracts the distance (estimated branch length) of each",
552
+ "placed read to a given node in a JPlace file."],
553
+ "requires": [ { "ruby_gem": "json" } ],
554
+ "help_arg": "--help",
555
+ "options": [
556
+ {
557
+ "name": "Input file",
558
+ "opt": "--in",
559
+ "arg": "in_file",
560
+ "mandatory": true,
561
+ "description": ".jplace input file containing the read placement."
562
+ },
563
+ {
564
+ "name": "Node",
565
+ "opt": "--node",
566
+ "arg": "string",
567
+ "mandatory": true,
568
+ "description": ["Index (number in curly brackets) of the node to",
569
+ "which distances should be measured."]
570
+ },
571
+ {
572
+ "name": "Output file",
573
+ "opt": "--out",
574
+ "arg": "out_file",
575
+ "mandatory": true,
576
+ "description": "Output file in tabular format."
577
+ },
578
+ {
579
+ "opt": "--in-node",
580
+ "arg": "string",
581
+ "description": ["Report only reads placed at this node or it's",
582
+ "children."]
583
+ },
584
+ {
585
+ "opt": "--quiet",
586
+ "description": "Run quietly (no STDERR output)."
587
+ }
588
+ ]
589
+ },
590
+ {
591
+ "task": "JPlace.to_iToL.rb",
592
+ "description": ["Generates iToL-compatible files from a .jplace file",
593
+ "(produced by RAxML's EPA or pplacer), that can be used to draw",
594
+ "pie-charts in the nodes of the reference tree."],
595
+ "requires": [ { "ruby_gem": "json" } ],
596
+ "help_arg": "--help",
597
+ "options": [
598
+ {
599
+ "name": "Input file",
600
+ "opt": "--in",
601
+ "arg": "in_file",
602
+ "mandatory": true,
603
+ "description": ".jplace input file containing the read placement."
604
+ },
605
+ {
606
+ "name": "Out base",
607
+ "opt": "--out",
608
+ "arg": "out_file",
609
+ "mandatory": true,
610
+ "description": "Base of the output files."
611
+ },
612
+ {
613
+ "opt": "--unique",
614
+ "arg": "string",
615
+ "description": ["Name of the dataset (if only one is used).",
616
+ "Conflicts with 'Regex' and 'Separator'."]
617
+ },
618
+ {
619
+ "opt": "--regex",
620
+ "arg": "string",
621
+ "description": ["Regular expression capturing the sample ID (named",
622
+ "dataset) in read names. By default: '^(?<dataset>.+?):.*'.",
623
+ "Conflicts with 'Separator'."]
624
+ },
625
+ {
626
+ "opt": "--separator",
627
+ "arg": "string",
628
+ "description": ["String separating the dataset name and the rest of",
629
+ "the read name. It assumes that the read name starts by the",
630
+ "dataset name. Conflicts with 'Regex'."]
631
+ },
632
+ {
633
+ "opt": "--metadata",
634
+ "arg": "in_file",
635
+ "description": ["Datasets metadata in tab-delimited format with a",
636
+ "header row. Valid headers: name (required), color (in Hex),",
637
+ "size (# reads), norm (any float)."]
638
+ },
639
+ {
640
+ "opt": "--norm",
641
+ "arg": "string",
642
+ "description": ["Normalization strategy. Must be one of:\n",
643
+ "none: Direct read counts are reported without normalization.\n",
644
+ "count (default): The counts are normalized (divided) by the total",
645
+ "counts per dataset.\n",
646
+ "size: The counts are normalized (divided) by the size column in",
647
+ "metadata (must be integer).\n",
648
+ "norm: The counts are normalized (divided) by the norm column in",
649
+ "metadata (can be any float)."]
650
+ },
651
+ {
652
+ "opt": "--old-itol",
653
+ "description": ["Generate output file using the old iToL format",
654
+ " (pre v3.0)."]
655
+ },
656
+ {
657
+ "opt": "--collapse",
658
+ "arg": "in_file",
659
+ "description": "Internal nodes to collapse (requires rootted tree)."
660
+ },
661
+ {
662
+ "opt": "--area",
663
+ "description": ["If set, the area of the pies is proportional to the",
664
+ "placements. Otherwise, the radius is."]
665
+ },
666
+ {
667
+ "opt": "--quiet",
668
+ "description": "Run quietly (no STDERR output)."
669
+ }
670
+ ]
671
+ },
672
+ {
673
+ "task": "MyTaxa.fragsByTax.pl",
674
+ "description": "Identifies fragments annotated as a taxon in MyTaxa.",
675
+ "help_arg": "",
676
+ "options": [
677
+ {
678
+ "name": "MyTaxa output",
679
+ "arg": "in_file",
680
+ "mandatory": true,
681
+ "description": "Classification file generated by MyTaxa."
682
+ },
683
+ {
684
+ "name": "Taxon",
685
+ "arg": "string",
686
+ "mandatory": true,
687
+ "description": "Taxon to look for."
688
+ },
689
+ {
690
+ "name": "Rank",
691
+ "arg": "string",
692
+ "description": "Rank of taxon (optional). By default: any rank."
693
+ },
694
+ ">",
695
+ {
696
+ "arg": "out_file",
697
+ "mandatory": true,
698
+ "description": "List of fragment IDs."
699
+ }
700
+ ]
701
+ },
702
+ {
703
+ "task": "MyTaxa.seq-taxrank.rb",
704
+ "description": ["Generates a simple tabular file with the classification",
705
+ "of each sequence at a given taxonomic rank from a MyTaxa output."],
706
+ "help_arg": "--help",
707
+ "options": [
708
+ {
709
+ "name": "MyTaxa",
710
+ "opt": "--mytaxa",
711
+ "arg": "in_file",
712
+ "mandatory": true,
713
+ "description": "Input MyTaxa file."
714
+ },
715
+ {
716
+ "opt": "--rank",
717
+ "arg": "string",
718
+ "default": "genus",
719
+ "description": "Taxonomic rank."
720
+ },
721
+ {
722
+ "opt": "--quiet",
723
+ "description": "Run quietly."
724
+ }
725
+ ]
726
+ },
727
+ {
728
+ "task": "Taxonomy.silva2ncbi.rb",
729
+ "description": ["Re-formats Silva taxonomy into NCBI-like taxonomy dump",
730
+ "files."],
731
+ "help_arg": "--help",
732
+ "options": [
733
+ {
734
+ "opt": "--silvaranks",
735
+ "arg": "in_file",
736
+ "mandatory": true,
737
+ "description": ["Input Silva ranks file (e.g.,",
738
+ "tax_ranks_ssu_115.txt)."],
739
+ "source_url": "https://www.arb-silva.de/no_cache/download/archive/release_115/Exports/"
740
+ },
741
+ {
742
+ "opt": "--silvaref",
743
+ "arg": "in_file",
744
+ "mandatory": true,
745
+ "description": ["Input Silva ref alignment file (e.g.,",
746
+ "SSURef_NR99_115_tax_silva_full_align_trunc.fasta)."],
747
+ "source_url": "https://www.arb-silva.de/no_cache/download/archive/release_115/Exports/"
748
+ },
749
+ {
750
+ "opt": "--patch",
751
+ "arg": "in_file",
752
+ "description": ["If passed, it replaces the paths specified in the",
753
+ "patch."],
754
+ "source_url": "http://enve-omics.ce.gatech.edu/data/public_misc/patch_Silva_SSU_r115.txt"
755
+ },
756
+ {
757
+ "opt": "--seqinfo",
758
+ "arg": "out_file",
759
+ "description": ["If passed, it creates a CSV seq-info file",
760
+ "compatible with taxtastic."]
761
+ },
762
+ {
763
+ "opt": "--taxfile",
764
+ "arg": "out_file",
765
+ "description": "If passed, it creates a simple TSV taxonomy file."
766
+ },
767
+ {
768
+ "opt": "--ncbi",
769
+ "arg": "out_dir",
770
+ "description": ["If passed, output folder for the NCBI dump files",
771
+ "(e.g., taxdmp)."]
772
+ },
773
+ {
774
+ "opt": "--warns",
775
+ "description": "Verbously display warnings."
776
+ }
777
+ ]
778
+ },
779
+ {
780
+ "task": "GFF.catsbj.pl",
781
+ "description": ["Generates a list of coordinates from a GFF table",
782
+ "concatenating the subject sequences."],
783
+ "help_arg": "-h",
784
+ "see_also": ["BlastTab.recplot2.R", "BlastTab.catsbj.pl"],
785
+ "options": [
786
+ {
787
+ "name": "Lim file",
788
+ "opt": "-L",
789
+ "arg": "out_file",
790
+ "description": ["An output file with the absolute coordinates of the",
791
+ "concatenated contigs. This is identical to the .lim file",
792
+ "generated by BlastTab.catsbj.pl."]
793
+ },
794
+ {
795
+ "name": "Inter-feature gaps",
796
+ "opt": "-i",
797
+ "description": ["Preserve exact coordinates and include",
798
+ "inter-feature windows as separate bins. By default, the",
799
+ "coordinates are set in the midpoint between features when",
800
+ "non-contiguous."]
801
+ },
802
+ {
803
+ "name": "Subset",
804
+ "opt": "-s",
805
+ "description": ["The FastA provided is to be treated as a subset of",
806
+ "the subject. By default, it expects all the contigs to be present",
807
+ "in the BLAST."]
808
+ },
809
+ {
810
+ "name": "Quiet",
811
+ "opt": "-q",
812
+ "description": "Run quietly."
813
+ },
814
+ {
815
+ "name": "Subject sequences",
816
+ "arg": "in_file",
817
+ "mandatory": true,
818
+ "description": "Subject sequences (contigs) in FastA format."
819
+ },
820
+ {
821
+ "name": "Features",
822
+ "arg": "in_file",
823
+ "mandatory": true,
824
+ "description": "Features to map in GFF."
825
+ }
826
+ ]
827
+ },
828
+ {
829
+ "task": "Table.prefScore.R",
830
+ "description": ["Estimate preference score of species based on occupancy",
831
+ "in biased sample sets."],
832
+ "help_arg": "--help",
833
+ "requires": [ { "r_package": "optparse" } ],
834
+ "options": [
835
+ {
836
+ "name": "Occupancy matrix",
837
+ "opt": "--x",
838
+ "arg": "in_file",
839
+ "description": ["A tab-delimited table of presence/absence (1/0)",
840
+ "with species as rows and samples as columns."],
841
+ "mandatory": true
842
+ },
843
+ {
844
+ "name": "Sample set",
845
+ "opt": "--set",
846
+ "arg": "in_file",
847
+ "description": ["A list of sample names that constitute the test",
848
+ "set, one per line."],
849
+ "mandatory": true
850
+ },
851
+ {
852
+ "opt": "--ignore",
853
+ "arg": "in_file",
854
+ "description": ["A list of species to exclude from the analysis,",
855
+ "one per line."]
856
+ },
857
+ {
858
+ "name": "Significance threshold",
859
+ "opt": "--signif-thr",
860
+ "arg": "float",
861
+ "description": "Absolute value of the significance threshold."
862
+ },
863
+ {
864
+ "opt": "--col-above",
865
+ "arg": "string",
866
+ "description": "Color for points significantly above zero.",
867
+ "default": "#941100"
868
+ },
869
+ {
870
+ "opt": "--col-equal",
871
+ "arg": "string",
872
+ "description": ["Color for points not significantly different from",
873
+ "zero."],
874
+ "default": "#BDBDBD"
875
+ },
876
+ {
877
+ "opt": "--col-below",
878
+ "arg": "string",
879
+ "description": "Color for points significantly below zero.",
880
+ "default": "#2F5496"
881
+ },
882
+ {
883
+ "name": "Output preference scores",
884
+ "arg": "out_file",
885
+ "description": "Output raw-text file with preference scores.",
886
+ "mandatory": true
887
+ },
888
+ {
889
+ "name": "Graphical utput",
890
+ "arg": "out_file",
891
+ "description": "Output PDF file with preference scores plot."
892
+ },
893
+ {
894
+ "name": "Width",
895
+ "arg": "float",
896
+ "description": "Width of the plot in inches (7 by default)."
897
+ },
898
+ {
899
+ "name": "Height",
900
+ "arg": "float",
901
+ "description": "Height of the plot in inches (7 by default)."
902
+ }
903
+ ]
904
+ }
905
+ ]
906
+ }