miga-base 1.2.15.1 → 1.2.15.3

data/utils/FastAAI/FastAAI-legacy/FastAAI

@@ -1,1336 +0,0 @@
-#!/usr/bin/env python3
-
-"""
-########################################################################
-# Author:	   Carlos Ruiz
-# Intitution:   Georgia Institute of Technology
-# Version:	  1.0
-# Date:		 Dec 10, 2020
-
-# Description: Calculates the average amino acid identity using k-mers
-from single copy genes. It is a faster version of the regular AAI (Blast
-or Diamond) and the hAAI implemented in MiGA.
-########################################################################
-"""
-
-################################################################################
-"""---0.0 Import Modules---"""
-import subprocess, argparse, multiprocessing, datetime, shutil
-import textwrap, pickle, gzip
-import numpy as np
-from tempfile import TemporaryDirectory
-from random import randint
-from pathlib import Path
-from sys import argv
-from sys import exit
-from functools import partial
-import time
-
-
-################################################################################
-"""---1.0 Define Functions---"""
-# --- Run prodigal ---
-# ------------------------------------------------------
-def run_prodigal(input_file):
-    """
-    Runs prodigal, compares translation tables and stores faa files
-
-    Arguments:
-       input_file -- Path to genome FastA file
-    
-    Returns:
-        output -- Path to amino acid fasta result
-    """
-    # Predict proteins with translation tables 4 and 11
-    file_path = Path(input_file)
-    filename = file_path.name
-    folder = file_path.parent
-    protein_output = folder / (filename + '.faa')
-    output_11 = folder / (filename + '.faa.11')
-    temp_output = folder / (filename + '.temp')
-    subprocess.call(["prodigal", "-i", str(file_path), "-a", str(output_11), 
-                    "-p", "meta", "-q", "-o", str(temp_output)])
-    output_4 = folder / (filename + '.faa.4')
-    temp_output = folder / (filename + '.temp')
-    subprocess.call(["prodigal", "-i", str(file_path), "-a", str(output_4), 
-                    "-p", "meta", "-g", "4", "-q", "-o", str(temp_output)])
-
-    # Compare translation tables
-    length_4 = 0
-    length_11 = 0
-    with open(output_4, 'r') as table_4:
-        for line in table_4:
-            if line.startswith(">"):
-                continue
-            else:
-                length_4 += len(line.strip())
-
-    with open(output_11, 'r') as table_11:
-        for line in table_11:
-            if line.startswith(">"):
-                continue
-            else:
-                length_11 += len(line.strip())
-    
-    if (length_4 / length_11) >= 1.1:
-        shutil.copy(output_4, protein_output)
-    else:
-        shutil.copy(str(output_11), str(protein_output))
-    
-    # Remove intermediate files
-    output_4.unlink()
-    output_11.unlink()
-    temp_output.unlink()
-
-    # Remove stop '*' codons from protein sequences
-    with open(protein_output, 'r') as final_protein, open(temp_output, 'w') as temporal_file:
-        for line in final_protein:
-            if line.startswith(">"):
-                temporal_file.write("{}".format(line))
-            else:
-                line = line.replace('*', '')
-                temporal_file.write("{}".format(line))
-    shutil.copy(str(temp_output), str(protein_output))
-    temp_output.unlink()
-
-    return str(protein_output)
-# ------------------------------------------------------
-
-# --- Run prodigal for viruses ---
-# ------------------------------------------------------
-def run_prodigal_virus(input_file):
-    """
-    Runs prodigal, compares translation tables and stores faa files
-
-    Arguments:
-       input_file -- Path to genome FastA file
-    
-    Returns:
-        output -- Path to amino acid fasta result
-    """
-    # Predict proteins with translation tables 4 and 11
-    file_path = Path(input_file)
-    filename = file_path.name
-    folder = file_path.parent
-    protein_output = folder / (filename + '.faa')
-    temp_output = folder / (filename + '.temp')
-    subprocess.call(["prodigal", "-i", str(file_path), "-a", str(protein_output), 
-                    "-p", "meta", "-q", "-o", str(temp_output)])
-    
-    # Remove intermediate files
-    temp_output.unlink()
-
-    # Remove stop '*' codons from protein sequences
-    with open(protein_output, 'r') as final_protein, open(temp_output, 'w') as temporal_file:
-        for line in final_protein:
-            if line.startswith(">"):
-                temporal_file.write("{}".format(line))
-            else:
-                line = line.replace('*', '')
-                temporal_file.write("{}".format(line))
-    shutil.copy(str(temp_output), str(protein_output))
-    temp_output.unlink()
-
-    return str(protein_output)
-# ------------------------------------------------------
-
-# --- Run hmmsearch ---
-# ------------------------------------------------------
-def run_hmmsearch(input_file):
-    """
-    Runs hmmsearch on the set of SCGs and select the
-    best Archaea or Bacterial model
-    
-    Arguments:
-        input_file -- Path to protein FastA file
-    
-    Returns:
-        output -- Path to hmmsearch hits table
-    """
-    file_path = Path(input_file)
-    folder = file_path.parent
-    name = file_path.name
-    hmm_output = folder / (name + '.hmm')
-    temp_output = folder / (name + '.temp')
-    script_path = Path(__file__)
-    script_dir = script_path.parent
-    hmm_complete_model = script_dir / "../00.Libraries/01.SCG_HMMs/Complete_SCG_DB.hmm"
-    subprocess.call(["hmmsearch", "--tblout", str(hmm_output), "-o", str(temp_output), "--cut_tc", "--cpu", "1",
-                    str(hmm_complete_model), str(file_path)])
-    temp_output.unlink()
-    return str(hmm_output)
-# ------------------------------------------------------
-
-# --- Filter HMM results for best matches ---
-# ------------------------------------------------------
-def hmm_filter(scg_hmm_file, keep):
-    """
-    Filters HMM results for best hits per protein
-    
-    Arguments:
-        SCG_HMM_file {file path} -- Path to HMM results file
-        keep {bool} -- Keep HMM files
-    
-    Returns:
-        outfile -- Path to filtered files
-    """
-    hmm_path = Path(scg_hmm_file)
-    name = hmm_path.name
-    folder = hmm_path.parent
-    outfile = folder / (name + '.filt')
-    hmm_hit_dict = {}
-    with open(scg_hmm_file, 'r') as hit_file:
-        for line in hit_file:
-            if line.startswith("#"):
-                continue
-            else:
-                hit = line.strip().split()
-                protein_name = hit[0]
-                score = float(hit[8])
-                if protein_name in hmm_hit_dict:
-                    if score > hmm_hit_dict[protein_name][0]:
-                        hmm_hit_dict[protein_name] = [score, line]
-                    elif score < hmm_hit_dict[protein_name][0]:
-                        continue
-                    else:
-                        if randint(2) > 0:
-                            hmm_hit_dict[protein_name] = [score, line]
-                else:
-                    hmm_hit_dict[protein_name] = [score, line]
-    with open(outfile, 'w') as output:
-        for hits in hmm_hit_dict.values():
-            output.write("{}".format(hits[1]))
-    return str(outfile)
-# ------------------------------------------------------
-
-# --- Find Kmers from HMM results ---
-# ------------------------------------------------------
-def kmer_extract(input_files):
-    """
-    Extract kmers from protein files that have hits
-    in the HMM searches.
-    
-    Arguments:
-        SCG_HMM_file {file path} -- Path to filtered HMM results.
-    
-    Returns:
-        [genome_kmers] -- Dictionary of kmers per gene. 
-    """
-    final_filename = input_files[0]
-    protein_file = input_files[1]
-    scg_hmm_file = input_files[2]
-    positive_matches = {}
-    positive_proteins = []
-    with open(scg_hmm_file, 'r') as hmm_input:
-        for line in hmm_input:
-            line = line.strip().split()
-            protein_name = line[0]
-            model_name = line[3]
-            score = float(line[8])
-            if model_name in positive_matches:
-                if score > positive_matches[model_name][1]:
-                    positive_matches[model_name] = [protein_name, score]
-                else:
-                    continue
-            else:
-                positive_matches[model_name] = [protein_name, score]
-    for proteins in positive_matches.values():
-        positive_proteins.append(proteins[0])
-    scg_kmers = read_kmers_from_file(protein_file, positive_proteins, 4)
-    for accession, protein in positive_matches.items():
-        scg_kmers[accession] = scg_kmers.pop(protein[0])
-    genome_kmers = {final_filename : scg_kmers}
-    return genome_kmers
-# ------------------------------------------------------
-
-# --- Extract kmers from protein sequences ---
-# ------------------------------------------------------
-def read_kmers_from_file(filename, positive_hits, ksize):
-    scg_kmers = {}
-    store_sequence = False
-    protein_name = ""
-    protein_sequence = ""
-    with open(filename) as fasta_in:
-        for line in fasta_in:
-            if line.startswith(">"):
-                if store_sequence == True:
-                    kmers = build_kmers(protein_sequence, ksize)
-                    scg_kmers[protein_name] = kmers
-                protein_sequence = ""
-                store_sequence = False
-                line = line.replace(">", "")
-                protein_name = line.strip().split()[0]
-                if protein_name in positive_hits:
-                    store_sequence = True
-            else:
-                if store_sequence == True:
-                    protein_sequence += line.strip()
-                else:
-                    continue
-            if store_sequence == True:
-                kmers = build_kmers(protein_sequence, ksize)
-                scg_kmers[protein_name] = kmers
-    return scg_kmers
-# ------------------------------------------------------
-
-# --- Extract kmers from viral protein sequences ---
-# ------------------------------------------------------
-def read_viral_kmers_from_file(input_information):
-    final_filename = input_information[0]
-    protein_file = input_information[1]
-    kmer_size = input_information[2]
-    
-    scg_kmers = set()
-    protein_sequence = ""
-    store_sequence = False
-    number_of_proteins = 0
-    with open(protein_file) as fasta_in:
-        for line in fasta_in:
-            if line.startswith(">"):
-                number_of_proteins += 1
-                if store_sequence == True:
-                    kmers = build_viral_kmers(protein_sequence, kmer_size)
-                    scg_kmers.update(kmers)
-                    protein_sequence = ""
-                else:
-                    protein_sequence = ""
-                    store_sequence = True
-            else:
-                protein_sequence += line.strip()
-            if store_sequence == True:
-                kmers = build_viral_kmers(protein_sequence, kmer_size)
-                scg_kmers.update(kmers)
-    genome_kmers = {final_filename : [number_of_proteins, ','.join(list(scg_kmers))]}
-    return genome_kmers
-# ------------------------------------------------------
-
-# --- Build Kmers ---
-# ------------------------------------------------------
-def build_kmers(sequence, ksize):
-    kmers = []
-    n_kmers = len(sequence) - ksize + 1
-
-    for i in range(n_kmers):
-        kmer = sequence[i:i + ksize]
-        kmers.append(kmer)
-    kmers_set = ','.join(set(kmers))
-    return kmers_set
-# ------------------------------------------------------
-
-# --- Build Viral Kmers ---
-# ------------------------------------------------------
-def build_viral_kmers(sequence, ksize):
-    kmers = []
-    n_kmers = len(sequence) - ksize + 1
-
-    for i in range(n_kmers):
-        kmer = sequence[i:i + ksize]
-        kmers.append(kmer)
-    kmers_set = set(kmers)
-    return kmers_set
-# ------------------------------------------------------
-
-# --- Create global dictionary with unique kmers and indices for each one ---
-# ------------------------------------------------------
-def global_unique_kmers(kmer_dictionaries):
-    """
-    Extract every kmer in the whole dataset
-    Create global dictionary with unique kmers and indices for each one
-    
-    Arguments:
-        kmer_dict {dict} -- Dictionary with kmers for each marker protein per input file
-    
-    Returns:
-        [global_kmer_index_dictionary] -- Dictionary with a unique index per kmer
-    """
-    # Make this dictionary global regardless of quer == reference or not
-    print("Indexing unique kmers")
-    global global_kmer_index_dictionary
-    global_kmer_index_dictionary = {}
-    counter = 0
-    for kmer_dict in kmer_dictionaries:
-        for marker_protein_id in kmer_dict.values():
-            for kmer_list in marker_protein_id.values():
-                kmer_list = kmer_list.split(',')
-                for kmer in kmer_list:
-                    try:
-                        global_kmer_index_dictionary[kmer]
-                    except:
-                        global_kmer_index_dictionary[kmer] = counter
-                        counter += 1
-# ------------------------------------------------------
-
-# --- Create global viral dictionary with unique kmers and indices for each one ---
-# ------------------------------------------------------
-def global_unique_viral_kmers(kmer_dictionaries):
-    """
-    Extract every kmer in the whole dataset
-    Create global dictionary with unique kmers and indices for each one
-    
-    Arguments:
-        kmer_dict {dict} -- Dictionary with kmers for each marker protein per input file
-    
-    Returns:
-        [global_kmer_index_dictionary] -- Dictionary with a unique index per kmer
-    """
-    # Make this dictionary global regardless of quer == reference or not
-    print("Indexing unique kmers")
-    global global_kmer_index_dictionary
-    global_kmer_index_dictionary = {}
-    counter = 0
-    for kmer_dict in kmer_dictionaries:
-        for kmer_list in kmer_dict.values():
-            for kmer in kmer_list[1].split(','):
-                try:
-                    global_kmer_index_dictionary[kmer]
-                except:
-                    global_kmer_index_dictionary[kmer] = counter
-                    counter += 1
-# ------------------------------------------------------
-
-# --- Convert kmers to indices ---
-# ------------------------------------------------------
-def convert_kmers_to_indices(kmer_dict):
-    print("Converting kmers to indices")
-    for genome in kmer_dict:
-        for protein_marker in kmer_dict[genome]:
-            kmer_index = []
-            for kmer in kmer_dict[genome][protein_marker].split(','):
-                kmer_index.append(global_kmer_index_dictionary[kmer])
-            kmer_index = np.sort(np.unique(np.array(kmer_index, dtype=np.int32)))
-            kmer_dict[genome][protein_marker] = kmer_index
-
-    return kmer_dict
-# ------------------------------------------------------
-
-# --- Convert viral kmers to indices ---
-# ------------------------------------------------------
-def convert_viral_kmers_to_indices(kmer_dict):
-    print("Converting kmers to indices")
-    for genome in kmer_dict:
-        kmer_index = []
-        for kmer in kmer_dict[genome][1].split(','):
-            kmer_index.append(global_kmer_index_dictionary[kmer])
-        kmer_index = np.sort(np.unique(np.array(kmer_index, dtype=np.int32)))
-        kmer_dict[genome][1] = kmer_index
-
-    return kmer_dict
-# ------------------------------------------------------
-
-# --- Transform kmer dictionaries to index dictionaries ---
-# ------------------------------------------------------
-def transform_kmer_dicts_to_arrays(kmer_dict, temporal_working_directory, single_dataset):
-    kmer_dict = convert_kmers_to_indices(kmer_dict)
-    #Get skip indices
-    smartargs = []
-    genome_ids = list(kmer_dict.keys())
-    for i in range(0, len(genome_ids)):
-        if single_dataset == True:
-            smartargs.append((temporal_working_directory, genome_ids[i], i))
-        else:
-            smartargs.append((temporal_working_directory, genome_ids[i]))
-        
-    return kmer_dict, smartargs
-# ------------------------------------------------------
-
-# --- Transform viral kmer dictionaries to index dictionaries ---
-# ------------------------------------------------------
-def transform_viral_kmer_dicts_to_arrays(kmer_dict, temporal_working_directory, single_dataset):
-    kmer_dict = convert_viral_kmers_to_indices(kmer_dict)
-    #Get skip indices
-    smartargs = []
-    genome_ids = list(kmer_dict.keys())
-    for i in range(0, len(genome_ids)):
-        if single_dataset == True:
-            smartargs.append((temporal_working_directory, genome_ids[i], i))
-        else:
-            smartargs.append((temporal_working_directory, genome_ids[i]))
-        
-    return kmer_dict, smartargs
-# ------------------------------------------------------
-
-# --- Parse kAAI when query == reference ---
-# ------------------------------------------------------
-def single_kaai_parser(arguments):
-    """
-    Calculates the Jaccard distances using single protein markers shared by two genomes
-    
-    Arguments:
-        arguments {tuple} -- Tuple with the temporal folder, the query id and the index of said query_id
-    
-    Returns:
-        [Path to output] -- Path to output file
-    """
-    temporal_folder = arguments[0]
-    query_id = arguments[1]
-    skip_first_n = arguments[2]
-    
-    temporal_folder = Path(str(temporal_folder.name))
-    temporal_file = Path(query_id).name + '.faai.temp'
-    temporal_output = temporal_folder / temporal_file
-    
-    query_scg_list = np.array(list(query_kmer_dictionary[query_id].keys()))
-    with open(temporal_output, 'w') as out_file:
-        #for target_genome, scg_ids in query_kmer_dictionary.items():
-        for target_genome in list(query_kmer_dictionary.keys())[skip_first_n:]:
-            # Get number and list of SCG detected in reference
-            target_scg_list = np.array(list(query_kmer_dictionary[target_genome].keys()))
-            shorter_genome = min(len(query_scg_list), len(target_scg_list))
-            #If self, 1.0 similarity.
-            if query_id == target_genome:
-                    out_file.write("{}\t{}\t{}\t{}\t{}\t{}\t{}\n".format(query_id, target_genome,
-                    1.0, 0.0, len(query_scg_list), len(target_scg_list), 100))
-                    continue
-            
-            jaccard_similarities = []
-            # Get shared proteins (scgs)
-            final_scg_list = np.intersect1d(query_scg_list, target_scg_list)
-            # Extract a list of kmers for each SCG in the list
-            query_kmer_list = list(map(query_kmer_dictionary[query_id].get, final_scg_list))
-            reference_kmer_list = list(map(query_kmer_dictionary[target_genome].get, final_scg_list))
-            # Calculate the jaccard index
-            for accession in range(len(query_kmer_list)):
-                union = len(np.union1d(query_kmer_list[accession], reference_kmer_list[accession]))
-                intersection = len(query_kmer_list[accession]) + len(reference_kmer_list[accession]) - union
-                jaccard_similarities.append(intersection / union)
-            
-            # Allow for numpy in-builts; they're a little faster.
-            if len(jaccard_similarities) > 0:
-                jaccard_similarities = np.array(jaccard_similarities, dtype=np.float_)
-                try:
-                    mean = np.mean(jaccard_similarities)
-                    var = np.std(jaccard_similarities)
-                    if mean >= 0.9:
-                        aai_est = ">90%"
-                    elif mean == 0:
-                        aai_est = "<30%"
-                    else:
-                        aai_est = kaai_to_aai(mean)
-                    out_file.write("{}\t{}\t{}\t{}\t{}\t{}\t{}\n".format(query_id, target_genome,
-                            round(mean, 4), round(var, 4),
-                            len(jaccard_similarities), shorter_genome, aai_est))
-                except:
-                    out_file.write("{}\t{}\t{}\t{}\t{}\t{}\t{}\n".format(query_id, target_genome,
-                            "NA", "NA", "NA", "NA", "NA"))
-            else:
-                out_file.write("{}\t{}\t{}\t{}\t{}\t{}\t{}\n".format(query_id, target_genome,
-                            "NA", "NA", "NA", "NA", "NA"))
-    return temporal_output
-# ------------------------------------------------------
-
-# --- Parse viral kAAI when query == reference ---
-# ------------------------------------------------------
-def single_virus_kaai_parser(arguments):
-    """
-    Calculates Jaccard distances on kmers from viral proteins
-    
-    Arguments:
-        query_id {str} -- Id of the query genome
-    
-    Returns:
-        [Path to output] -- Path to output file
-    """
-
-    temporal_folder = arguments[0]
-    query_id = arguments[1]
-    skip_first_n = arguments[2]
-
-    temporal_folder = Path(str(temporal_folder.name))
-    temporal_file = Path(query_id).name + '.faai.temp'
-    temporal_output = temporal_folder / temporal_file
-    # Get query kmers
-    proteins_query = query_kmer_dictionary[query_id][0]
-    kmers_query = query_kmer_dictionary[query_id][1]
-
-    # Start comparison with all genomes in the query dictionary
-    with open(temporal_output, 'w') as out_file:
-        for target_genome in list(query_kmer_dictionary.keys())[skip_first_n:]:
-            # If self, 1.0 similarity
-            if query_id == target_genome:
-                out_file.write("{}\t{}\t{}\t{}\t{}\n".format(query_id, target_genome,
-                    1.0, proteins_query, proteins_query))
-                continue
-
-            jaccard_index = None
-            proteins_reference = query_kmer_dictionary[target_genome][0]
-            kmers_reference = query_kmer_dictionary[target_genome][1]
-            # Calculate the Jaccard Index
-            union = len(np.union1d(kmers_query, kmers_reference))
-            intersection = len(kmers_query) + len(kmers_reference) - union
-            jaccard_index = intersection/union
-            out_file.write("{}\t{}\t{}\t{}\t{}\n".format(query_id, target_genome,
-                    jaccard_index, proteins_query, proteins_reference))
-    return temporal_output
-# ------------------------------------------------------
-
-# --- Parse kAAI when query != reference ---
-# ------------------------------------------------------
-def double_kaai_parser(arguments):
-    """
-    Calculates the Jaccard distances using single protein markers shared by two genomes
-    
-    Arguments:
-        arguments {tuple} -- Tuple with the temporal folder, the query id and the index of said query_id
-    
-    Returns:
-        [Path to output] -- Path to output file
-    """
-    temporal_folder = arguments[0]
-    query_id = arguments[1]
-    
-    temporal_folder = Path(str(temporal_folder.name))
-    temporal_file = Path(query_id).name + '.faai.temp'
-    temporal_output = temporal_folder / temporal_file
-
-    query_scg_list = np.array(list(query_kmer_dictionary[query_id].keys()))
-
-    with open(temporal_output, 'w') as out_file:
-        for target_genome in list(reference_kmer_dictionary.keys()):
-            # Get number and list of SCG detected in reference
-            target_scg_list = np.array(list(reference_kmer_dictionary[target_genome].keys()))
-            shorter_genome = min(len(query_scg_list), len(target_scg_list))
-            #If self, 1.0 similarity.
-            if query_id == target_genome:
-                    out_file.write("{}\t{}\t{}\t{}\t{}\t{}\t{}\n".format(query_id, target_genome,
-                    1.0, 0.0, len(query_scg_list), len(target_scg_list), 100))
-                    continue
-            
-            jaccard_similarities = []
-            # Get shared proteins (scgs)
-            final_scg_list = np.intersect1d(query_scg_list, target_scg_list)
-            # Extract a list of kmers for each SCG in the list
-            query_kmer_list = list(map(query_kmer_dictionary[query_id].get, final_scg_list))
-            reference_kmer_list = list(map(reference_kmer_dictionary[target_genome].get, final_scg_list))
-            # Calculate the jaccard index
-            for accession in range(len(query_kmer_list)):
-                union = len(np.union1d(query_kmer_list[accession], reference_kmer_list[accession]))
-                intersection = len(query_kmer_list[accession]) + len(reference_kmer_list[accession]) - union
-                jaccard_similarities.append(intersection / union)
-            
-            # Allow for numpy in-builts; they're a little faster.
-            if len(jaccard_similarities) > 0:
-                jaccard_similarities = np.array(jaccard_similarities, dtype=np.float_)
-                try:
-                    mean = np.mean(jaccard_similarities)
-                    var = np.std(jaccard_similarities)
-                    if mean >= 0.9:
-                        aai_est = ">90%"
-                    elif mean == 0:
-                        aai_est = "<30%"
-                    else:
-                        aai_est = kaai_to_aai(mean)
-                    out_file.write("{}\t{}\t{}\t{}\t{}\t{}\t{}\n".format(query_id, target_genome,
-                            round(mean, 4), round(var, 4),
-                            len(jaccard_similarities), shorter_genome, aai_est))
-                except:
-                    out_file.write("{}\t{}\t{}\t{}\t{}\t{}\t{}\n".format(query_id, target_genome,
-                            "NA", "NA", "NA", "NA", "NA"))
-            else:
-                out_file.write("{}\t{}\t{}\t{}\t{}\t{}\t{}\n".format(query_id, target_genome,
-                            "NA", "NA", "NA", "NA", "NA"))
-    return temporal_output
-# ------------------------------------------------------
-
-# --- Parse viral kAAI when query != reference ---
-# ------------------------------------------------------
-def double_viral_kaai_parser(arguments):
-    """
-    Calculates Jaccard distances on kmers from viral proteins
-    
-    Arguments:
-        query_id {str} -- Id of the query genome
-    
-    Returns:
-        [Path to output] -- Path to output file
-    """
-    temporal_folder = arguments[0]
-    query_id = arguments[1]
-
-    temporal_folder = Path(str(temporal_folder.name))
-    temporal_file = Path(query_id).name + '.faai.temp'
-    temporal_output = temporal_folder / temporal_file
-    # Get query kmers
-    proteins_query = query_kmer_dictionary[query_id][0]
-    kmers_query = query_kmer_dictionary[query_id][1]
-
-    # Start comparison with all genomes in the query dictionary
-    with open(temporal_output, 'w') as out_file:
-        for target_genome in reference_kmer_dictionary.keys():
-            # If self, 1.0 similarity
-            if query_id == target_genome:
-                out_file.write("{}\t{}\t{}\t{}\t{}\n".format(query_id, target_genome,
-                    1.0, proteins_query, proteins_query))
-                continue
-
-            jaccard_index = None
-            proteins_reference = reference_kmer_dictionary[target_genome][0]
-            kmers_reference = reference_kmer_dictionary[target_genome][1]
-            # Calculate the Jaccard Index
-            union = len(np.union1d(kmers_query, kmers_reference))
-            intersection = len(kmers_query) + len(kmers_reference) - union
-            jaccard_index = intersection/union
-            out_file.write("{}\t{}\t{}\t{}\t{}\n".format(query_id, target_genome,
-                    jaccard_index, proteins_query, proteins_reference))
-    return temporal_output
-# ------------------------------------------------------
-
-# --- Query == Reference initializer function ---
-# ------------------------------------------------------
-def single_dictionary_initializer(_dictionary):
-    """
-    Make dictionary available for multiprocessing
-    """
-    global query_kmer_dictionary
-    query_kmer_dictionary = _dictionary
-# ------------------------------------------------------
-
-# --- Query != Reference initializer function ---
-# ------------------------------------------------------
-def two_dictionary_initializer(_query_dictionary, _reference_dictionary):
-    """
-    Make dictionary available for multiprocessing
-    """
-    global query_kmer_dictionary
-    global reference_kmer_dictionary
-    query_kmer_dictionary = _query_dictionary
-    reference_kmer_dictionary = _reference_dictionary
-# ------------------------------------------------------
-
-# --- Merge kmer dictionaries ---
-# ------------------------------------------------------
-def merge_dicts(dictionaries):
-    """
-    Given any number of dicts, shallow copy and merge into a new dict,
-    precedence goes to key value pairs in latter dicts.
-    """
-    result = {}
-    for kmer_dictionary in dictionaries:
-        result.update(kmer_dictionary)
-    return result
-# ------------------------------------------------------
-
-# --- Merge kmer dictionaries ---
-# ------------------------------------------------------
-def kaai_to_aai(kaai):
-    # Transform the kAAI into estimated AAI values
-    aai_hat = (-0.3087057 + 1.810741 * (np.exp(-(-0.2607023 * np.log(kaai))**(1/3.435))))*100
-    return aai_hat
-# ------------------------------------------------------
-	
-
-################################################################################
-"""---2.0 Main Function---"""
-
-def main():
-    # Setup parser for arguments.
-    parser = argparse.ArgumentParser(formatter_class=argparse.RawTextHelpFormatter,
-            description='''This script calculates the average amino acid identity using k-mers\n'''
-                        '''from single copy genes. It is a faster version of the regular AAI '''
-                        '''(Blast or Diamond) and the hAAI implemented in MiGA.'''
-            '''Usage: ''' + argv[0] + ''' -p [Protein Files] -t [Threads] -o [Output]\n'''
-            '''Global mandatory parameters: -g [Genome Files] OR -p [Protein Files] OR -s [SCG HMM Results] -o [AAI Table Output]\n'''
-            '''Optional Database Parameters: See ''' + argv[0] + ' -h')
-    mandatory_options = parser.add_argument_group('Mandatory i/o options. You must select an option for the queries and one for the references.')
-    mandatory_options.add_argument('--qg', dest='query_genomes', action='store', required=False,
-                                    help='File with list of query genomes.')
-    mandatory_options.add_argument('--qp', dest='query_proteins', action='store', required=False,
-                                    help='File with list of query proteins.')
-    mandatory_options.add_argument('--qh', dest='query_hmms', action='store', required=False,
-                                    help=textwrap.dedent('''
-                                    File with list of pre-computed query hmmsearch results.
-                                    If you select this option you must also provide a file with 
-                                    a list of protein files for the queries (with --qp).
-                                    '''))
-    mandatory_options.add_argument('--qd', dest='query_database', action='store', required=False,
-                                    help='File with list of pre-indexed query databases.')
-    mandatory_options.add_argument('--rg', dest='reference_genomes', action='store', required=False,
-                                    help='File with list of reference genomes.')
-    mandatory_options.add_argument('--rp', dest='reference_proteins', action='store', required=False,
-                                    help='File with list of reference proteins.')
-    mandatory_options.add_argument('--rh', dest='reference_hmms', action='store', required=False,
-                                    help=textwrap.dedent('''
-                                    File with list of pre-computed reference hmmsearch results.
-                                    If you select this option you must also provide a file with 
-                                    a list of protein files for the references (with --qp).
-                                    '''))
-    mandatory_options.add_argument('--rd', dest='reference_database', action='store', required=False,
-                                    help='File with list of pre-indexed reference databases.')
-    mandatory_options.add_argument('-o', '--output', dest='output', action='store', required=False, help='Output file. By default kaai_comparisons.txt')
-    additional_input_options = parser.add_argument_group('Behavior modification options.')
-    additional_input_options.add_argument('-e', '--ext', dest='extension', action='store', required=False, 
-                                            help='Extension to remove from original filename, e.g. ".fasta"')
-    additional_input_options.add_argument('-i', '--index', dest='index_db', action='store_true', required=False, 
-                                            help='Only index and store databases, i.e., do not perform comparisons.')
-    additional_input_options.add_argument('-a', '--all-vs-all', dest='all_vs_all',
-                                    action='store_true', required=False,
-                                    help='Perform all-vs-all comparison, using only query input.')
-    additional_input_options.add_argument('--input-paths', dest='input_paths',
-                                    action='store_true', required=False,
-                                    help='The input files are direct paths to the data, not lists of files.')
-    misc_options = parser.add_argument_group('Miscellaneous options')
-    misc_options.add_argument('--virus', dest='virus', action='store_true', required=False,
-                                help='Toggle virus-virus comparisons. Use only with viral genomes or proteins.')
-    misc_options.add_argument('-t', '--threads', dest='threads', action='store', default=1, type=int, required=False,
-                                help='Number of threads to use, by default 1')
-    misc_options.add_argument('-k', '--keep', dest='keep', action='store_false', required=False,
-                                help='Keep intermediate files, by default true')
-
-    args = parser.parse_args()
-
-    query_genomes = args.query_genomes
-    query_proteins = args.query_proteins
-    query_hmms = args.query_hmms
-    query_database = args.query_database
-    if args.all_vs_all:
-        reference_genomes = query_genomes
-        reference_proteins = query_proteins
-        reference_hmms = query_hmms
-        reference_database = query_database
-    else:
-        reference_genomes = args.reference_genomes
-        reference_proteins = args.reference_proteins
-        reference_hmms = args.reference_hmms
-        reference_database = args.reference_database
-    output = args.output
-    if output == None:
-        output == "kaai_comparisons.txt"
-    extension = args.extension
-    index_db = args.index_db
-    threads = args.threads
-    keep = args.keep
-    virus = args.virus
-    input_paths = args.input_paths
-
-    print("FastAAI started on {}".format(datetime.datetime.now()))
-    # Check user input
-    # ------------------------------------------------------
-    # Check if no query was provided
-    if query_genomes == None and query_proteins == None and query_hmms == None and query_database == None:
-        exit('Please prove a file with a list of queries, e.g., --qg, --qp, --qh, or --qd)')
-    # Check query inputs
-    query_input = None
-    if query_hmms != None:
-        if virus == True:
-            exit("If you are comparing viruses, please start from the genome or protein files.")
-        query_input = query_hmms
-        if query_proteins != None:
-            print("Starting from query hmmsearch results.")
-            print("You also provided the list of protein files used for hmmsearch.")
-        elif query_proteins == None:
-            print("You chose to start from pre-computed hmmsearch results for your queries (--qh).")
-            print("However, I also need the location of the query proteins used for hmmsearch.")
-            exit("Please provide them with --qp.")
-    elif query_proteins != None:
-        query_input = query_proteins
-        print("Starting from query proteins.")
-    elif query_genomes != None:
-        query_input = query_genomes
-        print("Starting from query genomes.")
-    elif query_database != None:
-        query_input = query_database
-        print("Starting from the pre-indexed query database.")
-    # Check if no reference was provided
-    if reference_genomes == None and reference_proteins == None and reference_hmms == None and reference_database == None:
-        exit('Please prove a file with a list of references, e.g., --rg, --rp, --rh, or --rd)')
-    # Check reference inputs
-    reference_input = None
-    if reference_hmms != None:
-        if virus == True:
-            exit("If you are comparing viruses, please start from the genome or protein files.")
-        reference_input = reference_hmms
-        if reference_proteins != None:
-            print("Starting from reference hmmsearch results.")
-            print("You also provided the list of protein files used for hmmsearch.")
-        elif reference_proteins == None:
-            print("You chose to start from pre-computed hmmsearch results for your references (--rh).")
-            print("However, I also need the location of the query proteins used for hmmsearch.")
-            exit("Please provide them with --rp.")
-    elif reference_proteins != None:
-        reference_input = reference_proteins
-        print("Starting from reference proteins.")
-    elif reference_genomes != None:
-        reference_input = reference_genomes
-        print("Starting from reference genomes.")
-    elif reference_database != None:
-        reference_input = reference_database
-        print("Starting from the pre-indexed reference database.")
-    # ------------------------------------------------------
-
-    # Create temporal working directory
-    temporal_working_directory = TemporaryDirectory()
-    # ------------------------------------------------------
-
-    # Check if queries are the same as references (an all-vs-all comparison)
-    # ------------------------------------------------------
-    same_inputs = False
-    if query_input == reference_input:
-        same_inputs = True
-    if same_inputs == True:
-        print('You specified the same query and reference files.')
-        print('I will perform an all vs all comparison :)')
-    # ------------------------------------------------------
-
-    #* Database Parsing is the same regardless of bacterial or viral genomes
-    # If using pre-indexed databases, check if they are valid files.
-    # ------------------------------------------------------
-    # If any of the starting points is from database, then store the
-    # kmer structures in the corresponding dictionaries.
-    # Otherwise read the file list and get the filenames
-    query_kmer_dict = None
-    query_kmer_dict_list = []
-    reference_kmer_dict = None
-    reference_kmer_dict_list = []
-    query_database_files = []
-    reference_database_files = []
-    if query_database != None:
-        if input_paths == True:
-            query_database_files.append(query_database)
-        else:
-            with open(query_database) as database_files:
-                for db_location in database_files:
-                    query_database_files.append(db_location)
-    if reference_database != None:
-        if input_paths == True:
-            reference_database_files.append(reference_database)
-        else:
-            with open(reference_database) as database_files:
-                for db_location in database_files:
-                    reference_database_files.append(db_location)
-
-    # If starting from database and query == reference
-    if same_inputs == True:
-        if query_database != None:
-            for db_location in query_database_files:
-                if Path(db_location.strip()).is_file():
-                    with gzip.open(db_location.strip(), 'rb') as database_handle:
-                        temp_dict = pickle.load(database_handle)
-                        if isinstance(temp_dict,dict):
-                            query_kmer_dict_list.append(temp_dict)
-                                                            #Carlos, this line serves no purpose but does take a bunch of time and mem.
-                            #print(query_kmer_dict_list)
-                        else:
-                            exit("One of the database files appear to have the wrong format. Please provide a correctly formated database.")
-            query_kmer_dict = merge_dicts(query_kmer_dict_list)
-    else:
-    # If the inputs are not the same:
-        # If query and ref are provided
-        if query_database != None and reference_database != None:
-            for db_location in query_database_files:
-                if Path(db_location.strip()).is_file():
-                    with gzip.open(db_location.strip(), 'rb') as database_handle:
-                        temp_dict = pickle.load(database_handle)
-                        if isinstance(temp_dict,dict):
-                            query_kmer_dict_list.append(temp_dict)
-                        else:
-                            exit("One of the query database files appear to have the wrong format. Please provide a correctly formated database.")
-            query_kmer_dict = merge_dicts(query_kmer_dict_list)
-            for db_location in reference_database_files:
-                if Path(db_location.strip()).is_file():
-                    with gzip.open(db_location.strip(), 'rb') as database_handle:
-                        temp_dict = pickle.load(database_handle)
-                        if isinstance(temp_dict,dict):
-                            reference_kmer_dict_list.append(temp_dict)
-                        else:
-                            exit("One of the reference database files appear to have the wrong format. Please provide a correctly formated database.")
-            reference_kmer_dict = merge_dicts(reference_kmer_dict_list)
-        # If only the query has a db
-        elif query_database != None and reference_database == None:
-            for db_location in query_database_files:
-                if Path(db_location.strip()).is_file():
-                    with gzip.open(db_location.strip(), 'rb') as database_handle:
-                        temp_dict = pickle.load(database_handle)
-                        if isinstance(temp_dict,dict):
-                            query_kmer_dict_list.append(temp_dict)
-                        else:
-                            exit("One of the query database files appear to have the wrong format. Please provide a correctly formated database.")
-            query_kmer_dict = merge_dicts(query_kmer_dict_list)
-        # If only the reference has a db
-        elif query_database == None and reference_database != None:
-            for db_location in reference_database_files:
-                if Path(db_location.strip()).is_file():
-                    with gzip.open(db_location.strip(), 'rb') as database_handle:
-                        temp_dict = pickle.load(database_handle)
-                        if isinstance(temp_dict,dict):
-                            reference_kmer_dict_list.append(temp_dict)
-                        else:
-                            exit("One of the reference database files appear to have the wrong format. Please provide a correctly formated database.")
-            reference_kmer_dict = merge_dicts(reference_kmer_dict_list)
-    # ------------------------------------------------------
-
-    # Get files from the query and reference lists and then
-    # create a dictionary with resulting filenames and a list with dictionary keys
-    # The structure of the dictionary is:
-    # original_query, proteins, hmms, filtered_hmms
-    # ------------------------------------------------------
-    # First parse the query:
-    query_list = []
-    query_file_names = {}
-    # For bacterial genomes
-    if virus == False:
-        if query_database != None:
-            pass
-        else:
-            if input_paths == True:
-              query_list.append(query_input)
-            else:
-                with open(query_input, 'r') as query_input_fh:
-                    for line in query_input_fh:
-                        query_list.append(line.strip())
-            for index, query in enumerate(query_list):
-                query_name = str(Path(query).name)
-                if extension != None:
-                    query_name = query_name.replace(extension, "")
-                if query_hmms != None:
-                    query_protein_list = []
-                    with open(query_proteins, 'r') as query_protein_fh:
-                        for line in query_protein_fh:
-                            query_protein_list.append(line.strip())
-                    query_file_names[query_name] = [None, query_protein_list[index], query, query + '.filt']
-                elif query_proteins != None:
-                    query_file_names[query_name] = [None, query, query + '.hmm', query + '.hmm.filt']
-                elif query_genomes != None:
-                    query_file_names[query_name] = [query, query + '.faa', query + '.faa.hmm', query + '.faa.hmm.filt']
-    # For viral genomes
-    else:
-        if query_database != None:
-            pass
-        else:
-            if input_paths == True:
-              query_list.append(query_input)
-            else:
-                with open(query_input, 'r') as query_input_fh:
-                    for line in query_input_fh:
-                        query_list.append(line.strip())
-            for index, query in enumerate(query_list):
-                query_name = str(Path(query).name)
-                if extension != None:
-                    query_name = query_name.replace(extension, "")
-                if query_proteins != None:
-                    query_file_names[query_name] = [None, query]
-                elif query_genomes != None:
-                    query_file_names[query_name] = [query, query + '.faa']
-
-    # Then parse the references:
-    reference_list = []
-    reference_file_names = {}
-    if same_inputs == True:
-            pass
-    else:
-        # For bacterial genomes
-        if virus == False:
-            if reference_database != None:
-                pass
-            else:
-                if input_paths == True:
-                    reference_list.append(reference_input)
-                else:
-                    with open(reference_input, 'r') as reference_input_fh:
-                        for line in reference_input_fh:
-                            reference_list.append(line.strip())
-                for index, reference in enumerate(reference_list):
-                    reference_name = str(Path(reference).name)
-                    if extension != None:
-                        reference_name = reference_name.replace(extension, "")
-                    if reference_hmms != None:
-                        reference_protein_list = []
-                        with open(reference_proteins, 'r') as reference_protein_fh:
-                            for line in reference_protein_fh:
-                                reference_protein_list.append(line.strip())
-                        reference_file_names[reference_name] = [None, reference_protein_list[index], reference, reference + '.filt']
-                    elif reference_proteins != None:
-                        reference_file_names[reference_name] = [None, reference, reference + '.hmm', reference + '.hmm.filt']
-                    elif query_genomes != None:
-                        reference_file_names[reference_name] = [reference, reference + '.faa', reference + '.faa.hmm', reference + '.faa.hmm.filt']
-        # For viral genomes
-        else:
-            if reference_database != None:
-                pass
-            else:
-                if input_paths == True:
-                    reference_list.append(reference_input)
-                else:
-                    with open(reference_input, 'r') as reference_input_fh:
-                        for line in reference_input_fh:
-                            reference_list.append(line.strip())
-                for index, reference in enumerate(reference_list):
-                    reference_name = str(Path(reference).name)
-                    if extension != None:
-                        reference_name = reference_name.replace(extension, "")
-                    if reference_proteins != None:
-                        reference_file_names[reference_name] = [None, reference]
-                    elif query_genomes != None:
-                        reference_file_names[reference_name] = [reference, reference + '.faa']
-    # ------------------------------------------------------
-
-    # Pre-index and store databases
-    # ------------------------------------------------------
-    # Pre-index queries
-    if query_kmer_dict == None:
-        print("Processing queries...")
-        # If using bacterial genomes
-        if virus == False:
-            if query_hmms != None:
-                query_hmm_results = query_list
-            elif query_proteins != None:
-                query_protein_files = query_list
-                print("Searching against HMM models...")
-                try:
-                    pool = multiprocessing.Pool(threads)
-                    query_hmm_results = pool.map(run_hmmsearch, query_protein_files)
-                finally:
-                    pool.close()
-                    pool.join()
-            elif query_genomes != None:
-                print("Predicting proteins...")
-                # Predict query proteins 
-                try:
-                    pool = multiprocessing.Pool(threads)
-                    query_protein_files = pool.map(run_prodigal, query_list)
-                finally:
-                    pool.close()
-                    pool.join()
-                print("Done!")
-                print("Searching against HMM models...")
-                # Run hmmsearch against proteins predicted
-                try:
-                    pool = multiprocessing.Pool(threads)
-                    query_hmm_results = pool.map(run_hmmsearch, query_protein_files)
-                finally:
-                    pool.close()
-                    pool.join()
-                print("Done!")
-            print("Filtering query hmmsearch results...")
-            # Filter query HMM search results
-            try:
-                pool = multiprocessing.Pool(threads)
-                pool.map(partial(hmm_filter, keep=keep), query_hmm_results)
-            finally:
-                pool.close()
-                pool.join()
-            print("Extracting kmers from query proteins...")
-            # Finding kmers for all queries
-            query_information = []
-            for name, values in query_file_names.items():
-                query_information.append((name, values[1], values[3]))
-            try:
-                pool = multiprocessing.Pool(threads)
-                kmer_results = pool.map(kmer_extract, query_information)
-            finally:
-                pool.close()
-                pool.join()
-            query_kmer_dict = merge_dicts(kmer_results)
-            del kmer_results
-        # If using viral genomes
-        else:
-            if query_genomes != None:
-                print("Predicting proteins...")
-                # Predict query proteins 
-                try:
-                    pool = multiprocessing.Pool(threads)
-                    query_protein_files = pool.map(run_prodigal_virus, query_list)
-                finally:
-                    pool.close()
-                    pool.join()
-                print("Done!")
-            elif query_proteins != None:
-                query_protein_files = query_list
-            print("Extracting kmers from query proteins...")
-            query_information = []
-            for name, values in query_file_names.items():
-                query_information.append((name, values[1], 4))
-            try:
-                pool = multiprocessing.Pool(threads)
-                kmer_results = pool.map(read_viral_kmers_from_file, query_information)
-            finally:
-                pool.close()
-                pool.join()
-            query_kmer_dict = merge_dicts(kmer_results)
-            del kmer_results
-
-    # Pre-index references (if different from queries)
-    if same_inputs == False and reference_kmer_dict == None:
-        print("Processing references...")
-        # If using bacterial genomes
-        if virus == False:
-            if reference_hmms != None:
-                reference_hmm_results = reference_list
-            elif reference_proteins != None:
-                reference_protein_files = reference_list
-                print("Searching against HMM models...   ")
-                try:
-                    pool = multiprocessing.Pool(threads)
-                    reference_hmm_results = pool.map(run_hmmsearch, reference_protein_files)
-                finally:
-                    pool.close()
-                    pool.join()
-            if reference_genomes != None:
-                print("Predicting proteins...")
-                # Predict reference proteins 
-                try:
-                    pool = multiprocessing.Pool(threads)
-                    reference_protein_files = pool.map(run_prodigal, reference_list)
-                finally:
-                    pool.close()
-                    pool.join()
-                print("Done!")
-                print("Searching against HMM models...")
-                # Run hmmsearch against proteins predicted
-                try:
-                    pool = multiprocessing.Pool(threads)
-                    reference_hmm_results = pool.map(run_hmmsearch, reference_protein_files)
-                finally:
-                    pool.close()
-                    pool.join()
-                print("Done!")
-            print("Filtering reference hmmsearch results...")
-            # Filter reference HMM search results
-            try:
-                pool = multiprocessing.Pool(threads)
-                pool.map(partial(hmm_filter, keep=keep), reference_hmm_results)
-            finally:
-                pool.close()
-                pool.join()
-            print("Extracting kmers from reference proteins...") 
-            # Finding kmers for all queries
-            reference_information = []
-            for name, values in reference_file_names.items():
-                reference_information.append((name, values[1], values[3]))
-            try:
-                pool = multiprocessing.Pool(threads)
-                kmer_results = pool.map(kmer_extract, reference_information)
-            finally:
-                pool.close()
-                pool.join()
-            reference_kmer_dict = merge_dicts(kmer_results)
-            del kmer_results
-        # If using viral genomes
-        else:
-            if query_genomes != None:
-                print("Predicting proteins...")
-                # Predict query proteins 
-                try:
-                    pool = multiprocessing.Pool(threads)
-                    query_protein_files = pool.map(run_prodigal, query_list)
-                finally:
-                    pool.close()
-                    pool.join()
-                print("Done!")
-            elif query_proteins != None:
-                query_protein_files = query_list
-            print("Extracting kmers from query proteins...")
-            reference_information = []
-            for name, values in reference_file_names.items():
-                reference_information.append((name, values[1], 4))
-            try:
-                pool = multiprocessing.Pool(threads)
-                kmer_results = pool.map(read_viral_kmers_from_file, reference_information)
-            finally:
-                pool.close()
-                pool.join()
-            reference_kmer_dict = merge_dicts(kmer_results)
-            del kmer_results
-    # ------------------------------------------------------
-    
-    # Create or database(s) and compress it(them)
-    # ------------------------------------------------------
-    if same_inputs == True and query_database == None:
-        print("Saving pre-indexed database...")
-        query_database_name = query_input + '.db.gz'
-        with gzip.open(query_database_name, 'wb') as database_handle:
-            pickle.dump(query_kmer_dict, database_handle, protocol=4)
-    if same_inputs == False and query_database == None and reference_database == None:
-        print("Saving pre-indexed databases...")
-        query_database_name = query_input + '.db.gz'
-        reference_database_name = reference_input + '.db.gz'
-        with gzip.open(query_database_name, 'wb') as database_handle:
-            pickle.dump(query_kmer_dict, database_handle, protocol=4)
-        with gzip.open(reference_database_name, 'wb') as database_handle:
-            pickle.dump(reference_kmer_dict, database_handle, protocol=4)
-    elif same_inputs == False and query_database == None:
-        print("Saving pre-indexed query database...")
-        query_database_name = query_input + '.db.gz'
-        with gzip.open(query_database_name, 'wb') as database_handle:
-            pickle.dump(query_kmer_dict, database_handle, protocol=4)
-    elif same_inputs == False and reference_database == None:
-        print("Saving pre-indexed reference database...")
-        reference_database_name = reference_input + '.db.gz'
-        with gzip.open(reference_database_name, 'wb') as database_handle:
-            pickle.dump(reference_kmer_dict, database_handle, protocol=4)
-    # ------------------------------------------------------
-    # Calculate Jaccard distances
-    # ------------------------------------------------------
-    if index_db == True:
-        print("Finished pre-indexing databases.")
-        print("Next time you can run the program using only these files with --qd and(or) --rd.")
-    else:
-        print("Calculating shared kmer fraction...")
-        if virus == False:
-            if same_inputs == True:
-                # Create global kmer index dictionary "global_kmer_index_dictionary"
-                print(temporal_working_directory)
-                global_unique_kmers([query_kmer_dict])
-                query_kmer_dict, query_smart_args_tempdir = transform_kmer_dicts_to_arrays(query_kmer_dict, temporal_working_directory, single_dataset=True)
-                print("Beginning FastAAI pairwise calculations now.")
-                try:
-                    pool = multiprocessing.Pool(threads, initializer = single_dictionary_initializer, initargs = (query_kmer_dict,))
-                    Fraction_Results = pool.map(single_kaai_parser, query_smart_args_tempdir)
-                finally:
-                    pool.close()
-                    pool.join()
-            else:
-                print(temporal_working_directory)
-                global_unique_kmers([query_kmer_dict, reference_kmer_dict])
-                query_kmer_dict, query_smart_args_tempdir = transform_kmer_dicts_to_arrays(query_kmer_dict, temporal_working_directory, single_dataset=False)
-                reference_kmer_dict, _ref_smart_args_tempdir = transform_kmer_dicts_to_arrays(reference_kmer_dict, temporal_working_directory, single_dataset=False)
-                print("Beginning FastAAI pairwise calculations now.")
-                try:
-                    pool = multiprocessing.Pool(threads, initializer = two_dictionary_initializer, initargs = (query_kmer_dict, reference_kmer_dict))
-                    Fraction_Results = pool.map(double_kaai_parser, query_smart_args_tempdir)
-                finally:
-                    pool.close()
-                    pool.join()
-        else:
-            if same_inputs == True:
-                print(temporal_working_directory)
-                global_unique_viral_kmers([query_kmer_dict])
-                query_kmer_dict, query_smart_args_tempdir = transform_viral_kmer_dicts_to_arrays(query_kmer_dict, temporal_working_directory, single_dataset=True)
-                print("Beginning FastAAI pairwise calculations now.")
-                try:
-                    pool = multiprocessing.Pool(threads, initializer = single_dictionary_initializer, initargs = (query_kmer_dict,))
-                    Fraction_Results = pool.map(single_virus_kaai_parser, query_smart_args_tempdir)
-                finally:
-                    pool.close()
-                    pool.join()
-            else:
-                print(temporal_working_directory)
-                global_unique_viral_kmers([query_kmer_dict, reference_kmer_dict])
-                query_kmer_dict, query_smart_args_tempdir = transform_viral_kmer_dicts_to_arrays(query_kmer_dict, temporal_working_directory, single_dataset=False)
-                reference_kmer_dict, _ref_smart_args_tempdir = transform_viral_kmer_dicts_to_arrays(reference_kmer_dict, temporal_working_directory, single_dataset=False)
-                print("Beginning FastAAI pairwise calculations now.")
-                try:
-                    pool = multiprocessing.Pool(threads, initializer = two_dictionary_initializer, initargs = (query_kmer_dict, reference_kmer_dict))
-                    Fraction_Results = pool.map(double_viral_kaai_parser, query_smart_args_tempdir)
-                finally:
-                    pool.close()
-                    pool.join()
-    # ------------------------------------------------------
-    
-    # Merge results into a single output
-    # ------------------------------------------------------
-        print("Merging results...")
-        print(temporal_working_directory)
-        with open(output, 'w') as outfile:
-            for file in Fraction_Results:
-                with open(file) as Temp:
-                    shutil.copyfileobj(Temp, outfile)
-                file.unlink()
-        print("FastAAI finishied correctly on {}".format(datetime.datetime.now()))
-    # ------------------------------------------------------
-    # If comparing viral genomes
-
-
-	
-	
-	
-if __name__ == "__main__":
-    main()