miga-base 0.3.0.0 → 0.3.0.1
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- checksums.yaml +4 -4
- data/README.md +21 -4
- data/actions/init.rb +258 -0
- data/actions/run_local.rb +1 -2
- data/actions/test_taxonomy.rb +4 -1
- data/bin/miga +8 -1
- data/lib/miga/dataset.rb +4 -4
- data/lib/miga/dataset_result.rb +7 -4
- data/lib/miga/version.rb +2 -2
- data/scripts/_distances_noref_nomulti.bash +3 -1
- data/scripts/clade_finding.bash +1 -1
- data/scripts/init.bash +1 -1
- data/scripts/miga.bash +1 -1
- data/scripts/mytaxa.bash +78 -72
- data/scripts/mytaxa_scan.bash +67 -62
- data/scripts/ogs.bash +1 -1
- data/scripts/trimmed_fasta.bash +4 -3
- data/utils/enveomics/Examples/aai-matrix.bash +66 -0
- data/utils/enveomics/Examples/ani-matrix.bash +66 -0
- data/utils/enveomics/Examples/essential-phylogeny.bash +105 -0
- data/utils/enveomics/Examples/unus-genome-phylogeny.bash +100 -0
- data/utils/enveomics/LICENSE.txt +73 -0
- data/utils/enveomics/Makefile +52 -0
- data/utils/enveomics/Manifest/Tasks/aasubs.json +103 -0
- data/utils/enveomics/Manifest/Tasks/blasttab.json +703 -0
- data/utils/enveomics/Manifest/Tasks/distances.json +161 -0
- data/utils/enveomics/Manifest/Tasks/fasta.json +571 -0
- data/utils/enveomics/Manifest/Tasks/fastq.json +208 -0
- data/utils/enveomics/Manifest/Tasks/graphics.json +126 -0
- data/utils/enveomics/Manifest/Tasks/ogs.json +339 -0
- data/utils/enveomics/Manifest/Tasks/other.json +746 -0
- data/utils/enveomics/Manifest/Tasks/remote.json +355 -0
- data/utils/enveomics/Manifest/Tasks/sequence-identity.json +454 -0
- data/utils/enveomics/Manifest/Tasks/tables.json +308 -0
- data/utils/enveomics/Manifest/Tasks/trees.json +68 -0
- data/utils/enveomics/Manifest/Tasks/variants.json +111 -0
- data/utils/enveomics/Manifest/categories.json +132 -0
- data/utils/enveomics/Manifest/examples.json +154 -0
- data/utils/enveomics/Manifest/tasks.json +4 -0
- data/utils/enveomics/Pipelines/assembly.pbs/CONFIG.mock.bash +69 -0
- data/utils/enveomics/Pipelines/assembly.pbs/FastA.N50.pl +56 -0
- data/utils/enveomics/Pipelines/assembly.pbs/FastA.filterN.pl +60 -0
- data/utils/enveomics/Pipelines/assembly.pbs/FastA.length.pl +38 -0
- data/utils/enveomics/Pipelines/assembly.pbs/README.md +189 -0
- data/utils/enveomics/Pipelines/assembly.pbs/RUNME-2.bash +112 -0
- data/utils/enveomics/Pipelines/assembly.pbs/RUNME-3.bash +23 -0
- data/utils/enveomics/Pipelines/assembly.pbs/RUNME-4.bash +44 -0
- data/utils/enveomics/Pipelines/assembly.pbs/RUNME.bash +50 -0
- data/utils/enveomics/Pipelines/assembly.pbs/kSelector.R +37 -0
- data/utils/enveomics/Pipelines/assembly.pbs/newbler.pbs +68 -0
- data/utils/enveomics/Pipelines/assembly.pbs/newbler_preparator.pl +49 -0
- data/utils/enveomics/Pipelines/assembly.pbs/soap.pbs +80 -0
- data/utils/enveomics/Pipelines/assembly.pbs/stats.pbs +57 -0
- data/utils/enveomics/Pipelines/assembly.pbs/velvet.pbs +63 -0
- data/utils/enveomics/Pipelines/blast.pbs/01.pbs.bash +38 -0
- data/utils/enveomics/Pipelines/blast.pbs/02.pbs.bash +73 -0
- data/utils/enveomics/Pipelines/blast.pbs/03.pbs.bash +21 -0
- data/utils/enveomics/Pipelines/blast.pbs/BlastTab.recover_job.pl +72 -0
- data/utils/enveomics/Pipelines/blast.pbs/CONFIG.mock.bash +98 -0
- data/utils/enveomics/Pipelines/blast.pbs/FastA.split.pl +55 -0
- data/utils/enveomics/Pipelines/blast.pbs/README.md +127 -0
- data/utils/enveomics/Pipelines/blast.pbs/RUNME.bash +109 -0
- data/utils/enveomics/Pipelines/blast.pbs/TASK.check.bash +128 -0
- data/utils/enveomics/Pipelines/blast.pbs/TASK.dry.bash +16 -0
- data/utils/enveomics/Pipelines/blast.pbs/TASK.eo.bash +22 -0
- data/utils/enveomics/Pipelines/blast.pbs/TASK.pause.bash +26 -0
- data/utils/enveomics/Pipelines/blast.pbs/TASK.run.bash +89 -0
- data/utils/enveomics/Pipelines/blast.pbs/sentinel.pbs.bash +29 -0
- data/utils/enveomics/Pipelines/idba.pbs/README.md +49 -0
- data/utils/enveomics/Pipelines/idba.pbs/RUNME.bash +95 -0
- data/utils/enveomics/Pipelines/idba.pbs/run.pbs +56 -0
- data/utils/enveomics/Pipelines/trim.pbs/README.md +54 -0
- data/utils/enveomics/Pipelines/trim.pbs/RUNME.bash +70 -0
- data/utils/enveomics/Pipelines/trim.pbs/run.pbs +130 -0
- data/utils/enveomics/README.md +40 -0
- data/utils/enveomics/Scripts/AAsubs.log2ratio.rb +171 -0
- data/utils/enveomics/Scripts/Aln.cat.rb +162 -0
- data/utils/enveomics/Scripts/Aln.convert.pl +35 -0
- data/utils/enveomics/Scripts/AlphaDiversity.pl +152 -0
- data/utils/enveomics/Scripts/BlastPairwise.AAsubs.pl +102 -0
- data/utils/enveomics/Scripts/BlastTab.addlen.rb +61 -0
- data/utils/enveomics/Scripts/BlastTab.advance.bash +48 -0
- data/utils/enveomics/Scripts/BlastTab.best_hit_sorted.pl +55 -0
- data/utils/enveomics/Scripts/BlastTab.catsbj.pl +106 -0
- data/utils/enveomics/Scripts/BlastTab.cogCat.rb +76 -0
- data/utils/enveomics/Scripts/BlastTab.filter.pl +47 -0
- data/utils/enveomics/Scripts/BlastTab.kegg_pep2path_rest.pl +194 -0
- data/utils/enveomics/Scripts/BlastTab.metaxaPrep.pl +104 -0
- data/utils/enveomics/Scripts/BlastTab.pairedHits.rb +157 -0
- data/utils/enveomics/Scripts/BlastTab.recplot2.R +40 -0
- data/utils/enveomics/Scripts/BlastTab.seqdepth.pl +86 -0
- data/utils/enveomics/Scripts/BlastTab.seqdepth_ZIP.pl +119 -0
- data/utils/enveomics/Scripts/BlastTab.seqdepth_nomedian.pl +86 -0
- data/utils/enveomics/Scripts/BlastTab.subsample.pl +47 -0
- data/utils/enveomics/Scripts/BlastTab.sumPerHit.pl +114 -0
- data/utils/enveomics/Scripts/BlastTab.taxid2taxrank.pl +90 -0
- data/utils/enveomics/Scripts/BlastTab.topHits_sorted.rb +101 -0
- data/utils/enveomics/Scripts/Chao1.pl +97 -0
- data/utils/enveomics/Scripts/CharTable.classify.rb +234 -0
- data/utils/enveomics/Scripts/EBIseq2tax.rb +83 -0
- data/utils/enveomics/Scripts/FastA.N50.pl +56 -0
- data/utils/enveomics/Scripts/FastA.filter.pl +52 -0
- data/utils/enveomics/Scripts/FastA.filterLen.pl +28 -0
- data/utils/enveomics/Scripts/FastA.filterN.pl +60 -0
- data/utils/enveomics/Scripts/FastA.fragment.rb +92 -0
- data/utils/enveomics/Scripts/FastA.gc.pl +42 -0
- data/utils/enveomics/Scripts/FastA.interpose.pl +87 -0
- data/utils/enveomics/Scripts/FastA.length.pl +38 -0
- data/utils/enveomics/Scripts/FastA.per_file.pl +36 -0
- data/utils/enveomics/Scripts/FastA.qlen.pl +57 -0
- data/utils/enveomics/Scripts/FastA.rename.pl +65 -0
- data/utils/enveomics/Scripts/FastA.revcom.pl +23 -0
- data/utils/enveomics/Scripts/FastA.slider.pl +85 -0
- data/utils/enveomics/Scripts/FastA.split.pl +55 -0
- data/utils/enveomics/Scripts/FastA.subsample.pl +131 -0
- data/utils/enveomics/Scripts/FastA.tag.rb +64 -0
- data/utils/enveomics/Scripts/FastA.wrap.rb +48 -0
- data/utils/enveomics/Scripts/FastQ.filter.pl +54 -0
- data/utils/enveomics/Scripts/FastQ.interpose.pl +90 -0
- data/utils/enveomics/Scripts/FastQ.offset.pl +90 -0
- data/utils/enveomics/Scripts/FastQ.split.pl +53 -0
- data/utils/enveomics/Scripts/FastQ.tag.rb +63 -0
- data/utils/enveomics/Scripts/FastQ.toFastA.awk +24 -0
- data/utils/enveomics/Scripts/GenBank.add_fields.rb +84 -0
- data/utils/enveomics/Scripts/HMM.essential.rb +254 -0
- data/utils/enveomics/Scripts/HMMsearch.extractIds.rb +83 -0
- data/utils/enveomics/Scripts/JPlace.distances.rb +88 -0
- data/utils/enveomics/Scripts/JPlace.to_iToL.rb +306 -0
- data/utils/enveomics/Scripts/M5nr.getSequences.rb +81 -0
- data/utils/enveomics/Scripts/MeTaxa.distribution.pl +198 -0
- data/utils/enveomics/Scripts/MyTaxa.fragsByTax.pl +35 -0
- data/utils/enveomics/Scripts/MyTaxa.seq-taxrank.rb +49 -0
- data/utils/enveomics/Scripts/NCBIacc2tax.rb +92 -0
- data/utils/enveomics/Scripts/Newick.autoprune.R +27 -0
- data/utils/enveomics/Scripts/RAxML-EPA.to_iToL.pl +228 -0
- data/utils/enveomics/Scripts/RefSeq.download.bash +48 -0
- data/utils/enveomics/Scripts/SRA.download.bash +50 -0
- data/utils/enveomics/Scripts/TRIBS.plot-test.R +36 -0
- data/utils/enveomics/Scripts/TRIBS.test.R +39 -0
- data/utils/enveomics/Scripts/Table.barplot.R +30 -0
- data/utils/enveomics/Scripts/Table.df2dist.R +30 -0
- data/utils/enveomics/Scripts/Table.filter.pl +61 -0
- data/utils/enveomics/Scripts/Table.merge.pl +77 -0
- data/utils/enveomics/Scripts/Table.replace.rb +69 -0
- data/utils/enveomics/Scripts/Table.round.rb +63 -0
- data/utils/enveomics/Scripts/Table.split.pl +57 -0
- data/utils/enveomics/Scripts/Taxonomy.silva2ncbi.rb +227 -0
- data/utils/enveomics/Scripts/VCF.KaKs.rb +147 -0
- data/utils/enveomics/Scripts/VCF.SNPs.rb +88 -0
- data/utils/enveomics/Scripts/aai.rb +373 -0
- data/utils/enveomics/Scripts/ani.rb +362 -0
- data/utils/enveomics/Scripts/gi2tax.rb +103 -0
- data/utils/enveomics/Scripts/in_silico_GA_GI.pl +96 -0
- data/utils/enveomics/Scripts/lib/data/essential.hmm.gz +0 -0
- data/utils/enveomics/Scripts/lib/enveomics_rb/enveomics.rb +26 -0
- data/utils/enveomics/Scripts/lib/enveomics_rb/jplace.rb +253 -0
- data/utils/enveomics/Scripts/lib/enveomics_rb/og.rb +182 -0
- data/utils/enveomics/Scripts/lib/enveomics_rb/remote_data.rb +74 -0
- data/utils/enveomics/Scripts/lib/enveomics_rb/seq_range.rb +237 -0
- data/utils/enveomics/Scripts/lib/enveomics_rb/stat.rb +30 -0
- data/utils/enveomics/Scripts/lib/enveomics_rb/vcf.rb +135 -0
- data/utils/enveomics/Scripts/ogs.annotate.rb +88 -0
- data/utils/enveomics/Scripts/ogs.core-pan.rb +160 -0
- data/utils/enveomics/Scripts/ogs.extract.rb +125 -0
- data/utils/enveomics/Scripts/ogs.mcl.rb +186 -0
- data/utils/enveomics/Scripts/ogs.rb +104 -0
- data/utils/enveomics/Scripts/ogs.stats.rb +131 -0
- data/utils/enveomics/Scripts/rbm.rb +137 -0
- data/utils/enveomics/Tests/Makefile +10 -0
- data/utils/enveomics/Tests/Mgen_M2288.faa +3189 -0
- data/utils/enveomics/Tests/Mgen_M2288.fna +8282 -0
- data/utils/enveomics/Tests/Mgen_M2321.fna +8288 -0
- data/utils/enveomics/Tests/Nequ_Kin4M.faa +2970 -0
- data/utils/enveomics/Tests/Xanthomonas_oryzae-PilA.tribs.Rdata +0 -0
- data/utils/enveomics/Tests/Xanthomonas_oryzae-PilA.txt +7 -0
- data/utils/enveomics/Tests/Xanthomonas_oryzae.aai-mat.tsv +17 -0
- data/utils/enveomics/Tests/Xanthomonas_oryzae.aai.tsv +137 -0
- data/utils/enveomics/Tests/a_mg.cds-go.blast.tsv +123 -0
- data/utils/enveomics/Tests/a_mg.reads-cds.blast.tsv +200 -0
- data/utils/enveomics/Tests/a_mg.reads-cds.counts.tsv +55 -0
- data/utils/enveomics/Tests/alkB.nwk +1 -0
- data/utils/enveomics/Tests/anthrax-cansnp-data.tsv +13 -0
- data/utils/enveomics/Tests/anthrax-cansnp-key.tsv +17 -0
- data/utils/enveomics/Tests/hiv1.faa +59 -0
- data/utils/enveomics/Tests/hiv1.fna +134 -0
- data/utils/enveomics/Tests/hiv2.faa +70 -0
- data/utils/enveomics/Tests/hiv_mix-hiv1.blast.tsv +233 -0
- data/utils/enveomics/Tests/hiv_mix-hiv1.blast.tsv.lim +1 -0
- data/utils/enveomics/Tests/hiv_mix-hiv1.blast.tsv.rec +233 -0
- data/utils/enveomics/Tests/phyla_counts.tsv +10 -0
- data/utils/enveomics/Tests/primate_lentivirus.ogs +11 -0
- data/utils/enveomics/Tests/primate_lentivirus.rbm/hiv1-hiv1.rbm +9 -0
- data/utils/enveomics/Tests/primate_lentivirus.rbm/hiv1-hiv2.rbm +8 -0
- data/utils/enveomics/Tests/primate_lentivirus.rbm/hiv1-siv.rbm +6 -0
- data/utils/enveomics/Tests/primate_lentivirus.rbm/hiv2-hiv2.rbm +9 -0
- data/utils/enveomics/Tests/primate_lentivirus.rbm/hiv2-siv.rbm +6 -0
- data/utils/enveomics/Tests/primate_lentivirus.rbm/siv-siv.rbm +6 -0
- data/utils/enveomics/build_enveomics_r.bash +44 -0
- data/utils/enveomics/enveomics.R/DESCRIPTION +31 -0
- data/utils/enveomics/enveomics.R/NAMESPACE +35 -0
- data/utils/enveomics/enveomics.R/R/autoprune.R +121 -0
- data/utils/enveomics/enveomics.R/R/barplot.R +165 -0
- data/utils/enveomics/enveomics.R/R/cliopts.R +119 -0
- data/utils/enveomics/enveomics.R/R/df2dist.R +117 -0
- data/utils/enveomics/enveomics.R/R/growthcurve.R +263 -0
- data/utils/enveomics/enveomics.R/R/recplot.R +320 -0
- data/utils/enveomics/enveomics.R/R/recplot2.R +745 -0
- data/utils/enveomics/enveomics.R/R/tribs.R +423 -0
- data/utils/enveomics/enveomics.R/R/utils.R +16 -0
- data/utils/enveomics/enveomics.R/README.md +52 -0
- data/utils/enveomics/enveomics.R/data/growth.curves.rda +0 -0
- data/utils/enveomics/enveomics.R/data/phyla.counts.rda +0 -0
- data/utils/enveomics/enveomics.R/man/enve.GrowthCurve-class.Rd +30 -0
- data/utils/enveomics/enveomics.R/man/enve.TRIBS-class.Rd +43 -0
- data/utils/enveomics/enveomics.R/man/enve.TRIBS.merge.Rd +19 -0
- data/utils/enveomics/enveomics.R/man/enve.TRIBStest-class.Rd +37 -0
- data/utils/enveomics/enveomics.R/man/enve.__prune.iter.Rd +24 -0
- data/utils/enveomics/enveomics.R/man/enve.__prune.reduce.Rd +24 -0
- data/utils/enveomics/enveomics.R/man/enve.__tribs.Rd +33 -0
- data/utils/enveomics/enveomics.R/man/enve.barplot.Rd +64 -0
- data/utils/enveomics/enveomics.R/man/enve.cliopts.Rd +37 -0
- data/utils/enveomics/enveomics.R/man/enve.col.alpha.Rd +19 -0
- data/utils/enveomics/enveomics.R/man/enve.col2alpha.Rd +18 -0
- data/utils/enveomics/enveomics.R/man/enve.df2dist.Rd +26 -0
- data/utils/enveomics/enveomics.R/man/enve.df2dist.group.Rd +25 -0
- data/utils/enveomics/enveomics.R/man/enve.df2dist.list.Rd +26 -0
- data/utils/enveomics/enveomics.R/man/enve.growthcurve.Rd +49 -0
- data/utils/enveomics/enveomics.R/man/enve.prune.dist.Rd +28 -0
- data/utils/enveomics/enveomics.R/man/enve.recplot.Rd +97 -0
- data/utils/enveomics/enveomics.R/man/enve.recplot2-class.Rd +40 -0
- data/utils/enveomics/enveomics.R/man/enve.recplot2.Rd +40 -0
- data/utils/enveomics/enveomics.R/man/enve.recplot2.__counts.Rd +24 -0
- data/utils/enveomics/enveomics.R/man/enve.recplot2.__findPeak.Rd +40 -0
- data/utils/enveomics/enveomics.R/man/enve.recplot2.__findPeaks.Rd +18 -0
- data/utils/enveomics/enveomics.R/man/enve.recplot2.__peakHist.Rd +22 -0
- data/utils/enveomics/enveomics.R/man/enve.recplot2.__whichClosestPeak.Rd +20 -0
- data/utils/enveomics/enveomics.R/man/enve.recplot2.changeCutoff.Rd +18 -0
- data/utils/enveomics/enveomics.R/man/enve.recplot2.corePeak.Rd +18 -0
- data/utils/enveomics/enveomics.R/man/enve.recplot2.extractWindows.Rd +27 -0
- data/utils/enveomics/enveomics.R/man/enve.recplot2.findPeaks.Rd +53 -0
- data/utils/enveomics/enveomics.R/man/enve.recplot2.peak-class.Rd +44 -0
- data/utils/enveomics/enveomics.R/man/enve.tribs.Rd +44 -0
- data/utils/enveomics/enveomics.R/man/enve.tribs.test.Rd +21 -0
- data/utils/enveomics/enveomics.R/man/enveomics.R-package.Rd +15 -0
- data/utils/enveomics/enveomics.R/man/growth.curves.Rd +14 -0
- data/utils/enveomics/enveomics.R/man/phyla.counts.Rd +13 -0
- data/utils/enveomics/enveomics.R/man/plot.enve.GrowthCurve.Rd +43 -0
- data/utils/enveomics/enveomics.R/man/plot.enve.TRIBS.Rd +29 -0
- data/utils/enveomics/enveomics.R/man/plot.enve.TRIBStest.Rd +30 -0
- data/utils/enveomics/enveomics.R/man/plot.enve.recplot2.Rd +71 -0
- data/utils/enveomics/enveomics.R/man/summary.enve.GrowthCurve.Rd +18 -0
- data/utils/enveomics/enveomics.R/man/summary.enve.TRIBS.Rd +18 -0
- data/utils/enveomics/enveomics.R/man/summary.enve.TRIBStest.Rd +18 -0
- data/utils/enveomics/enveomics.R/man/z$-methods.Rd +27 -0
- data/utils/enveomics/globals.mk +8 -0
- data/utils/enveomics/manifest.json +9 -0
- data/utils/index_metadata.rb +0 -0
- data/utils/plot-taxdist.R +0 -0
- data/utils/requirements.txt +19 -19
- metadata +242 -2
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"tasks": [
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"description": ["Concatenates several multiple alignments in FastA",
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"name": "Input alignments",
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"arg": "in_file",
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"multiple_sep": " ",
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"mandatory": true,
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"description": "Input alignments in FastA format."
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},
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">",
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{
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"name": "Output alignment",
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"arg": "out_file",
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"mandatory": true,
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"description": "Output concatenated alignment."
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}
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]
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},
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{
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"task": "Aln.convert.pl",
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"description": "Translates between different alignment formats.",
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"help_arg": "",
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"requires": [
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{
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"perl_lib": "Bio::AlignIO"
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}
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],
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"cite": [["Stajich et al, 2002, GRes",
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"http://dx.doi.org/10.1101/gr.361602"]],
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"options": [
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{
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"name": "In-format",
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"description": "Input file's format.",
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"mandatory": true,
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"arg": "select",
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"values": ["bl2seq", "clustalw", "emboss", "fasta", "maf", "mase",
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"mega", "meme", "metafasta", "msf", "nexus", "pfam", "phylip", "po",
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"prodom", "psi", "selex", "stockholm", "XMFA", "arp"]
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},
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"name": "Out-format",
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"description": "Output file's format.",
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"mandatory": true,
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"arg": "select",
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"values": ["bl2seq", "clustalw", "emboss", "fasta", "maf", "mase",
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"mega", "meme", "metafasta", "msf", "nexus", "pfam", "phylip", "po",
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"prodom", "psi", "selex", "stockholm", "XMFA", "arp"]
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},
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"<",
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"arg": "in_file",
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"mandatory": true
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">",
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"mandatory": true
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}
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]
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},
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{
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"task": "AlphaDiversity.pl",
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"description": ["Takes a table of OTU abundance in one or more samples",
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"and calculates the Rao (Q_alpha), Rao-Jost (Q_alpha_eqv), Shannon",
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"(Hprime), and inverse Simpson (1_lambda) indices of alpha diversity",
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"for each sample."],
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"help_arg": "--help",
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"see_also": ["Chao1.pl"],
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"options": [
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{
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"name": "Input table",
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"opt": "-i",
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"arg": "in_file",
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"description": ["Columns: samples, rows: OTUs, first column: OTU",
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"names)."],
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"mandatory": true
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{
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"name": "Discard rows",
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"opt": "-r",
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"arg": "integer",
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"description": "Number of rows to ignore.",
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"default": 0
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},
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{
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"name": "Discard left columns",
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"opt": "-c",
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"arg": "integer",
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"description": ["Number of columns to ignore after the first column",
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"(i.e., between the first column, containing the name of the",
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"categories, and the first column of abundance values)."],
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"default": 0
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},
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{
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"name": "Discard right columns",
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"opt": "-C",
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"arg": "integer",
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"description": "Number of columns to ignore at the end of each line.",
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"default": 0
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},
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{
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"name": "Delimiter",
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"opt": "-d",
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"arg": "string",
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"description": "Character (or string) separating columns.",
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"note": ["Supported escaped characters are: '\\t' (tabulation), and",
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"'\\0' (null bit)."],
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"default": "\\t"
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},
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{
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"name": "Headers",
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"opt": "-h",
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"description": ["The first row is assumed to have the names of the",
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"samples."]
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},
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{
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"name": "Distances file",
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"opt": "-D",
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"arg": "in_file",
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"description": ["A squared matrix (or bottom-left half matrix) with",
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"the distances between categories (OTUs or functions). The first",
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"column must contain the names of the categories, and it shouldn't",
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"have headers. If not set, all distances are assumed to be one."],
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"note": "Only used for Rao."
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},
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{
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"name": "Skip Rao",
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"opt": "-R",
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"description": "Do not calculate Rao indices.",
|
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"note": ["This significantly decreases the total running time. Note",
|
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"that Rao indices are highly susceptible to precision errors, and",
|
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"shouldn't be trusted for very big numbers."]
|
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},
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{
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"name": "q for qD",
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"opt": "-q",
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"arg": "integer",
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"description": "Estimate the qD index (true diversity order q).",
|
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"default": 0
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},
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">",
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{
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"arg": "out_file",
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"mandatory": true
|
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}
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]
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},
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{
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"task": "Chao1.pl",
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"description": ["Takes a table of OTU abundance in one or more samples",
|
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"and calculates the chao1 index (with 95% Confidence Interval) for",
|
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"each sample. To use it with Qiime OTU Tables, run it ignoring 1",
|
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"left column and with header."],
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"see_also": ["AlphaDiversity.pl"],
|
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"help_arg": "--help",
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"options": [
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{
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"name": "Input file",
|
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"opt": "-i",
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"arg": "in_file",
|
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"mandatory": true,
|
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"description": "Input table (columns:samples, rows:OTUs)."
|
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},
|
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{
|
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"name": "Rows",
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"opt": "-r",
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"arg": "integer",
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"default": 0,
|
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"description": "Number of rows to ignore."
|
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},
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{
|
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"name": "Left columns to ignore",
|
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"opt": "-c",
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"arg": "integer",
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"default": 0,
|
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"description": "Number of columns to ignore."
|
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},
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{
|
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"name": "Right columns to ignore",
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"opt": "-C",
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"arg": "integer",
|
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"default": 0,
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"description": "Number of columns to ignore at the end."
|
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},
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{
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"name": "Delimiter",
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"opt": "-d",
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"arg": "string",
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"default": "\\t",
|
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"description": "Delimiter between columns.",
|
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"note": ["Supported escaped characters are: \\t (tabulation),",
|
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"and \\0 (null bit)."]
|
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},
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{
|
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"name": "Header",
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"opt": "-h",
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"description": ["If set, the first row is assumed to have the names",
|
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"of the samples."]
|
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},
|
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">",
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{
|
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"arg": "out_file",
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"mandatory": true,
|
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"description": ["Chao1 index and 95% CI bounds for each sample, in",
|
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"tab-delimited format."]
|
257
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}
|
258
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+
]
|
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},
|
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{
|
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"task": "CharTable.classify.rb",
|
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"description": ["Uses a dichotomous key to classify objects parsing a",
|
263
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"character table."],
|
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"help_arg": "--help",
|
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"options": [
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{
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"opt": "--table",
|
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"arg": "in_file",
|
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"mandatory": true,
|
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"description": ["Input table containing the states (columns) per",
|
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"object (row). It must be tab-delimited and with row and column",
|
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"names."]
|
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},
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{
|
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"opt": "--key",
|
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"arg": "in_file",
|
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"mandatory": true,
|
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"description": ["Input table containing the dychotomous key in",
|
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"linked style, defined in four columns (can contain #-lead comment",
|
280
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"lines): (1) ID of the step, typically a sequential integer. (2)",
|
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"Name of character to evaluate. Must coincide with the -t headers.",
|
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"(3) First character decision (see below). (4) Second character",
|
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|
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"decision (see below).\nA character decision must be formated as:",
|
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"state (must coincide with the values in -t), colon (:), step to",
|
285
|
+
"follow. If the state is * (star) any state triggers the decision",
|
286
|
+
"(this should be the norm in column 4). The step to follow should",
|
287
|
+
"be a step ID in square brackets, or the name of the",
|
288
|
+
"classification."]
|
289
|
+
},
|
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|
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{
|
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|
+
"opt": "--classification",
|
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"arg": "out_file",
|
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"description": ["Two-column table with the classification of the",
|
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|
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"input objects."]
|
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|
+
},
|
296
|
+
{
|
297
|
+
"opt": "--newick",
|
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|
+
"arg": "out_file",
|
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"description": ["Tree containing all the classified objects. This",
|
300
|
+
"only makes sense for synoptic keys."]
|
301
|
+
},
|
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+
{
|
303
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"opt": "--quiet",
|
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+
"description": "Run quietly (no STDERR output)."
|
305
|
+
}
|
306
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]
|
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|
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},
|
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{
|
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"task": "GenBank.add_fields.rb",
|
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"description": "Adds annotations to GenBank files.",
|
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"help_arg": "--help",
|
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"options": [
|
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{
|
314
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"opt": "--genbank",
|
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"arg": "in_file",
|
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|
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"mandatory": true,
|
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|
+
"description": "Input GenBank file."
|
318
|
+
},
|
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|
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{
|
320
|
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"opt": "--table",
|
321
|
+
"arg": "in_file",
|
322
|
+
"mandatory": true,
|
323
|
+
"description": ["Input file containing the annotations. It must be a",
|
324
|
+
"tab-delimited raw table including a header row with the names of",
|
325
|
+
"the fields."]
|
326
|
+
},
|
327
|
+
{
|
328
|
+
"opt": "--out",
|
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|
+
"arg": "out_file",
|
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|
+
"mandatory": true,
|
331
|
+
"description": "Output file containing the annotated GenBank."
|
332
|
+
},
|
333
|
+
{
|
334
|
+
"opt": "--key",
|
335
|
+
"arg": "integer",
|
336
|
+
"default": 1,
|
337
|
+
"description": "Key of the column to use as identifier."
|
338
|
+
},
|
339
|
+
{
|
340
|
+
"opt": "--split",
|
341
|
+
"arg": "string",
|
342
|
+
"default": "#",
|
343
|
+
"description": ["String that separates multiple entries in the",
|
344
|
+
"annotation features."]
|
345
|
+
},
|
346
|
+
{
|
347
|
+
"opt": "--quiet",
|
348
|
+
"description": "Run quietly (no STDERR output)."
|
349
|
+
}
|
350
|
+
]
|
351
|
+
},
|
352
|
+
{
|
353
|
+
"task": "HMM.essential.rb",
|
354
|
+
"description": ["Finds and extracts a collection of essential proteins",
|
355
|
+
"suitable for genome completeness evaluation and phylogenetic",
|
356
|
+
"analyses in Archaea and Bacteria."],
|
357
|
+
"warn": ["Most complete bacterial genomes contain only 106/111 genes in",
|
358
|
+
"this collection, therefore producing a completeness of 95.5%, and",
|
359
|
+
"most archaeal genomes only contain 26/111 genes, producing a",
|
360
|
+
"completeness of 23.4%. Use the options --bacteria and/or --archaea to",
|
361
|
+
"ignore models often missing in one or both domains. Note that even",
|
362
|
+
"with these options, some complete archaeal genomes result in very low",
|
363
|
+
"values of completeness (e.g., Nanoarchaeum equitans returns 88.5%)."],
|
364
|
+
"help_arg": "--help",
|
365
|
+
"requires": [
|
366
|
+
{
|
367
|
+
"description": "HMMer 3.0+",
|
368
|
+
"test": "hmmsearch -h",
|
369
|
+
"source_url": "http://hmmer.janelia.org/software"
|
370
|
+
}
|
371
|
+
],
|
372
|
+
"cite": [["Eddy, 2011, PLoS CB",
|
373
|
+
"http://dx.doi.org/10.1371/journal.pcbi.1002195"]],
|
374
|
+
"options": [
|
375
|
+
{
|
376
|
+
"name": "Input file",
|
377
|
+
"opt": "--in",
|
378
|
+
"arg": "in_file",
|
379
|
+
"mandatory": true,
|
380
|
+
"description": "FastA file containing all the proteins in the genome."
|
381
|
+
},
|
382
|
+
{
|
383
|
+
"name": "Output file",
|
384
|
+
"opt": "--out",
|
385
|
+
"arg": "out_file",
|
386
|
+
"description": ["FastA file with the translated essential genes. By",
|
387
|
+
"default the file is not produced."]
|
388
|
+
},
|
389
|
+
{
|
390
|
+
"opt": "--per-model",
|
391
|
+
"arg": "out_file",
|
392
|
+
"description": ["Prefix of translated genes in independent files",
|
393
|
+
"with the name of the model appended. By default files are not",
|
394
|
+
"produced."]
|
395
|
+
},
|
396
|
+
{
|
397
|
+
"opt": "--report",
|
398
|
+
"arg": "out_file",
|
399
|
+
"description": ["Path to the report file. By default, the report is",
|
400
|
+
"sent to the STDOUT."]
|
401
|
+
},
|
402
|
+
{
|
403
|
+
"opt": "--bacteria",
|
404
|
+
"description": "If set, ignores models typically missing in Bacteria."
|
405
|
+
},
|
406
|
+
{
|
407
|
+
"opt": "--archaea",
|
408
|
+
"description": "If set, ignores models typically missing in Archaea."
|
409
|
+
},
|
410
|
+
{
|
411
|
+
"opt": "--genome-eq",
|
412
|
+
"description": ["If set, ignores models not suitable for",
|
413
|
+
"genome-equivalents estimations. See Rodriguez-R et al, 2015, ISME",
|
414
|
+
"J 9(9):1928-1940."],
|
415
|
+
"source_url": "http://www.nature.com/ismej/journal/vaop/ncurrent/full/ismej20155a.html"
|
416
|
+
},
|
417
|
+
{
|
418
|
+
"opt": "--rename",
|
419
|
+
"arg": "string",
|
420
|
+
"description": ["If set, renames the sequences with the string",
|
421
|
+
"provided and appends it with pipe (|) and the gene name (except",
|
422
|
+
"in --per-model files)."]
|
423
|
+
},
|
424
|
+
{
|
425
|
+
"opt": "--no-stats",
|
426
|
+
"description": ["If set, no statistics are reported on genome",
|
427
|
+
"evaluation."]
|
428
|
+
},
|
429
|
+
{
|
430
|
+
"opt": "--no-genes",
|
431
|
+
"description": ["If set, statistics won't include the lists of",
|
432
|
+
"missing/multi-copy genes."]
|
433
|
+
},
|
434
|
+
{
|
435
|
+
"opt": "--metagenome",
|
436
|
+
"description": ["If set, it allows for multiple copies of each gene",
|
437
|
+
"and turns on metagenomic report mode."]
|
438
|
+
},
|
439
|
+
{
|
440
|
+
"opt": "--list-models",
|
441
|
+
"description": ["If set, it only lists the models and exits.",
|
442
|
+
"Compatible with 'Archaea', 'Bacteria', 'Genome eq', and 'Quiet';",
|
443
|
+
"ignores all other parameters."]
|
444
|
+
},
|
445
|
+
{
|
446
|
+
"opt": "--bin",
|
447
|
+
"arg": "in_dir",
|
448
|
+
"description": "Directory containing the binaries of HMMer 3.0+."
|
449
|
+
},
|
450
|
+
{
|
451
|
+
"opt": "--model-file",
|
452
|
+
"arg": "in_file",
|
453
|
+
"description": "External file containing models to search."
|
454
|
+
},
|
455
|
+
{
|
456
|
+
"opt": "--threads",
|
457
|
+
"arg": "integer",
|
458
|
+
"default": 2,
|
459
|
+
"description": "Number of parallel threads to be used."
|
460
|
+
},
|
461
|
+
{
|
462
|
+
"opt": "--quiet",
|
463
|
+
"description": "Run quietly (no STDERR output)."
|
464
|
+
}
|
465
|
+
]
|
466
|
+
},
|
467
|
+
{
|
468
|
+
"task": "HMMsearch.extractIds.rb",
|
469
|
+
"description": ["Extracts the sequence IDs and query model form a",
|
470
|
+
"(multiple) HMMsearch report (for HMMer 3.0)."],
|
471
|
+
"help_arg": "--help",
|
472
|
+
"options": [
|
473
|
+
{
|
474
|
+
"opt": "--all-evalue",
|
475
|
+
"arg": "float",
|
476
|
+
"description": "Maximum e-value of sequence to report result."
|
477
|
+
},
|
478
|
+
{
|
479
|
+
"opt": "--all-score",
|
480
|
+
"arg": "float",
|
481
|
+
"description": "Minimum score of sequence to report result."
|
482
|
+
},
|
483
|
+
{
|
484
|
+
"opt": "--best-evalue",
|
485
|
+
"arg": "float",
|
486
|
+
"description": "Maximum e-value of best domain to report result."
|
487
|
+
},
|
488
|
+
{
|
489
|
+
"opt": "--best-score",
|
490
|
+
"arg": "float",
|
491
|
+
"description": "Minimum score of best domain to report result."
|
492
|
+
},
|
493
|
+
{
|
494
|
+
"opt": "--no-model",
|
495
|
+
"description": "Do not include the query model in the output list."
|
496
|
+
},
|
497
|
+
{
|
498
|
+
"opt": "--quiet",
|
499
|
+
"description": "Run quietly."
|
500
|
+
},
|
501
|
+
"<",
|
502
|
+
{
|
503
|
+
"arg": "in_file",
|
504
|
+
"mandatory": true,
|
505
|
+
"description": "Input HMMsearch text file."
|
506
|
+
},
|
507
|
+
">",
|
508
|
+
{
|
509
|
+
"arg": "out_file",
|
510
|
+
"mandatory": true,
|
511
|
+
"description": "Output list of IDs."
|
512
|
+
}
|
513
|
+
]
|
514
|
+
},
|
515
|
+
{
|
516
|
+
"task": "JPlace.distances.rb",
|
517
|
+
"description": ["Extracts the distance (estimated branch length) of each",
|
518
|
+
"placed read to a given node in a JPlace file."],
|
519
|
+
"requires": [ { "ruby_gem": "json" } ],
|
520
|
+
"help_arg": "--help",
|
521
|
+
"options": [
|
522
|
+
{
|
523
|
+
"name": "Input file",
|
524
|
+
"opt": "--in",
|
525
|
+
"arg": "in_file",
|
526
|
+
"mandatory": true,
|
527
|
+
"description": ".jplace input file containing the read placement."
|
528
|
+
},
|
529
|
+
{
|
530
|
+
"name": "Node",
|
531
|
+
"opt": "--node",
|
532
|
+
"arg": "string",
|
533
|
+
"mandatory": true,
|
534
|
+
"description": ["Index (number in curly brackets) of the node to",
|
535
|
+
"which distances should be measured."]
|
536
|
+
},
|
537
|
+
{
|
538
|
+
"name": "Output file",
|
539
|
+
"opt": "--out",
|
540
|
+
"arg": "out_file",
|
541
|
+
"mandatory": true,
|
542
|
+
"description": "Output file in tabular format."
|
543
|
+
},
|
544
|
+
{
|
545
|
+
"opt": "--in-node",
|
546
|
+
"arg": "string",
|
547
|
+
"description": ["Report only reads placed at this node or it's",
|
548
|
+
"children."]
|
549
|
+
},
|
550
|
+
{
|
551
|
+
"opt": "--quiet",
|
552
|
+
"description": "Run quietly (no STDERR output)."
|
553
|
+
}
|
554
|
+
]
|
555
|
+
},
|
556
|
+
{
|
557
|
+
"task": "JPlace.to_iToL.rb",
|
558
|
+
"description": ["Generates iToL-compatible files from a .jplace file",
|
559
|
+
"(produced by RAxML's EPA or pplacer), that can be used to draw",
|
560
|
+
"pie-charts in the nodes of the reference tree."],
|
561
|
+
"requires": [ { "ruby_gem": "json" } ],
|
562
|
+
"help_arg": "--help",
|
563
|
+
"options": [
|
564
|
+
{
|
565
|
+
"name": "Input file",
|
566
|
+
"opt": "--in",
|
567
|
+
"arg": "in_file",
|
568
|
+
"mandatory": true,
|
569
|
+
"description": ".jplace input file containing the read placement."
|
570
|
+
},
|
571
|
+
{
|
572
|
+
"name": "Out base",
|
573
|
+
"opt": "--out",
|
574
|
+
"arg": "out_file",
|
575
|
+
"mandatory": true,
|
576
|
+
"description": "Base of the output files."
|
577
|
+
},
|
578
|
+
{
|
579
|
+
"opt": "--unique",
|
580
|
+
"arg": "string",
|
581
|
+
"description": ["Name of the dataset (if only one is used).",
|
582
|
+
"Conflicts with 'Regex' and 'Separator'."]
|
583
|
+
},
|
584
|
+
{
|
585
|
+
"opt": "--regex",
|
586
|
+
"arg": "string",
|
587
|
+
"description": ["Regular expression capturing the sample ID (named",
|
588
|
+
"dataset) in read names. By default: '^(?<dataset>.+?):.*'.",
|
589
|
+
"Conflicts with 'Separator'."]
|
590
|
+
},
|
591
|
+
{
|
592
|
+
"opt": "--separator",
|
593
|
+
"arg": "string",
|
594
|
+
"description": ["String separating the dataset name and the rest of",
|
595
|
+
"the read name. It assumes that the read name starts by the",
|
596
|
+
"dataset name. Conflicts with 'Regex'."]
|
597
|
+
},
|
598
|
+
{
|
599
|
+
"opt": "--metadata",
|
600
|
+
"arg": "in_file",
|
601
|
+
"description": ["Datasets metadata in tab-delimited format with a",
|
602
|
+
"header row. Valid headers: name (required), color (in Hex),",
|
603
|
+
"size (# reads), norm (any float)."]
|
604
|
+
},
|
605
|
+
{
|
606
|
+
"opt": "--norm",
|
607
|
+
"arg": "string",
|
608
|
+
"description": ["Normalization strategy. Must be one of:\n",
|
609
|
+
"none: Direct read counts are reported without normalization.\n",
|
610
|
+
"count (default): The counts are normalized (divided) by the total",
|
611
|
+
"counts per dataset.\n",
|
612
|
+
"size: The counts are normalized (divided) by the size column in",
|
613
|
+
"metadata (must be integer).\n",
|
614
|
+
"norm: The counts are normalized (divided) by the norm column in",
|
615
|
+
"metadata (can be any float)."]
|
616
|
+
},
|
617
|
+
{
|
618
|
+
"opt": "--old-itol",
|
619
|
+
"description": ["Generate output file using the old iToL format",
|
620
|
+
" (pre v3.0)."]
|
621
|
+
},
|
622
|
+
{
|
623
|
+
"opt": "--collapse",
|
624
|
+
"arg": "in_file",
|
625
|
+
"description": "Internal nodes to collapse (requires rootted tree)."
|
626
|
+
},
|
627
|
+
{
|
628
|
+
"opt": "--area",
|
629
|
+
"description": ["If set, the area of the pies is proportional to the",
|
630
|
+
"placements. Otherwise, the radius is."]
|
631
|
+
},
|
632
|
+
{
|
633
|
+
"opt": "--quiet",
|
634
|
+
"description": "Run quietly (no STDERR output)."
|
635
|
+
}
|
636
|
+
]
|
637
|
+
},
|
638
|
+
{
|
639
|
+
"task": "MyTaxa.fragsByTax.pl",
|
640
|
+
"description": "Identifies fragments annotated as a taxon in MyTaxa.",
|
641
|
+
"help_arg": "",
|
642
|
+
"options": [
|
643
|
+
{
|
644
|
+
"name": "MyTaxa output",
|
645
|
+
"arg": "in_file",
|
646
|
+
"mandatory": true,
|
647
|
+
"description": "Classification file generated by MyTaxa."
|
648
|
+
},
|
649
|
+
{
|
650
|
+
"name": "Taxon",
|
651
|
+
"arg": "string",
|
652
|
+
"mandatory": true,
|
653
|
+
"description": "Taxon to look for."
|
654
|
+
},
|
655
|
+
{
|
656
|
+
"name": "Rank",
|
657
|
+
"arg": "string",
|
658
|
+
"description": "Rank of taxon (optional). By default: any rank."
|
659
|
+
},
|
660
|
+
">",
|
661
|
+
{
|
662
|
+
"arg": "out_file",
|
663
|
+
"mandatory": true,
|
664
|
+
"description": "List of fragment IDs."
|
665
|
+
}
|
666
|
+
]
|
667
|
+
},
|
668
|
+
{
|
669
|
+
"task": "MyTaxa.seq-taxrank.rb",
|
670
|
+
"description": ["Generates a simple tabular file with the classification",
|
671
|
+
"of each sequence at a given taxonomic rank from a MyTaxa output."],
|
672
|
+
"help_arg": "--help",
|
673
|
+
"options": [
|
674
|
+
{
|
675
|
+
"name": "MyTaxa",
|
676
|
+
"opt": "--mytaxa",
|
677
|
+
"arg": "in_file",
|
678
|
+
"mandatory": true,
|
679
|
+
"description": "Input MyTaxa file."
|
680
|
+
},
|
681
|
+
{
|
682
|
+
"opt": "--rank",
|
683
|
+
"arg": "string",
|
684
|
+
"default": "genus",
|
685
|
+
"description": "Taxonomic rank."
|
686
|
+
},
|
687
|
+
{
|
688
|
+
"opt": "--quiet",
|
689
|
+
"description": "Run quietly."
|
690
|
+
}
|
691
|
+
]
|
692
|
+
},
|
693
|
+
{
|
694
|
+
"task": "Taxonomy.silva2ncbi.rb",
|
695
|
+
"description": ["Re-formats Silva taxonomy into NCBI-like taxonomy dump",
|
696
|
+
"files."],
|
697
|
+
"help_arg": "--help",
|
698
|
+
"options": [
|
699
|
+
{
|
700
|
+
"opt": "--silvaranks",
|
701
|
+
"arg": "in_file",
|
702
|
+
"mandatory": true,
|
703
|
+
"description": ["Input Silva ranks file (e.g.,",
|
704
|
+
"tax_ranks_ssu_115.txt)."],
|
705
|
+
"source_url": "https://www.arb-silva.de/no_cache/download/archive/release_115/Exports/"
|
706
|
+
},
|
707
|
+
{
|
708
|
+
"opt": "--silvaref",
|
709
|
+
"arg": "in_file",
|
710
|
+
"mandatory": true,
|
711
|
+
"description": ["Input Silva ref alignment file (e.g.,",
|
712
|
+
"SSURef_NR99_115_tax_silva_full_align_trunc.fasta)."],
|
713
|
+
"source_url": "https://www.arb-silva.de/no_cache/download/archive/release_115/Exports/"
|
714
|
+
},
|
715
|
+
{
|
716
|
+
"opt": "--patch",
|
717
|
+
"arg": "in_file",
|
718
|
+
"description": ["If passed, it replaces the paths specified in the",
|
719
|
+
"patch."],
|
720
|
+
"source_url": "http://enve-omics.ce.gatech.edu/data/public_misc/patch_Silva_SSU_r115.txt"
|
721
|
+
},
|
722
|
+
{
|
723
|
+
"opt": "--seqinfo",
|
724
|
+
"arg": "out_file",
|
725
|
+
"description": ["If passed, it creates a CSV seq-info file",
|
726
|
+
"compatible with taxtastic."]
|
727
|
+
},
|
728
|
+
{
|
729
|
+
"opt": "--taxfile",
|
730
|
+
"arg": "out_file",
|
731
|
+
"description": "If passed, it creates a simple TSV taxonomy file."
|
732
|
+
},
|
733
|
+
{
|
734
|
+
"opt": "--ncbi",
|
735
|
+
"arg": "out_dir",
|
736
|
+
"description": ["If passed, output folder for the NCBI dump files",
|
737
|
+
"(e.g., taxdmp)."]
|
738
|
+
},
|
739
|
+
{
|
740
|
+
"opt": "--warns",
|
741
|
+
"description": "Verbously display warnings."
|
742
|
+
}
|
743
|
+
]
|
744
|
+
}
|
745
|
+
]
|
746
|
+
}
|