bio-samtools 0.6.2 → 2.0.3
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- checksums.yaml +4 -4
- data/Gemfile +3 -2
- data/README.md +4 -7
- data/VERSION +1 -1
- data/bio-samtools.gemspec +47 -105
- data/doc/Bio.html +68 -131
- data/doc/Bio/DB.html +51 -111
- data/doc/Bio/DB/Alignment.html +135 -363
- data/doc/Bio/DB/Pileup.html +183 -170
- data/doc/Bio/DB/SAM.html +1396 -820
- data/doc/Bio/DB/SAM/Library.html +73 -123
- data/doc/Bio/DB/SAM/Tools.html +51 -273
- data/doc/Bio/DB/Tag.html +78 -124
- data/doc/Bio/DB/Vcf.html +111 -147
- data/doc/LICENSE_txt.html +113 -148
- data/doc/created.rid +9 -10
- data/doc/fonts.css +167 -0
- data/doc/fonts/Lato-Light.ttf +0 -0
- data/doc/fonts/Lato-LightItalic.ttf +0 -0
- data/doc/fonts/Lato-Regular.ttf +0 -0
- data/doc/fonts/Lato-RegularItalic.ttf +0 -0
- data/doc/fonts/SourceCodePro-Bold.ttf +0 -0
- data/doc/fonts/SourceCodePro-Regular.ttf +0 -0
- data/doc/images/add.png +0 -0
- data/doc/images/arrow_up.png +0 -0
- data/doc/images/delete.png +0 -0
- data/doc/images/tag_blue.png +0 -0
- data/doc/index.html +48 -54
- data/doc/js/darkfish.js +9 -22
- data/doc/js/search.js +20 -5
- data/doc/js/search_index.js +1 -1
- data/doc/rdoc.css +255 -218
- data/doc/table_of_contents.html +256 -137
- data/ext/Rakefile +57 -0
- data/lib/bio-samtools.rb +7 -2
- data/lib/bio/BIOExtensions.rb +89 -0
- data/lib/bio/db/alignment.rb +59 -0
- data/lib/bio/db/fastadb.rb +255 -0
- data/lib/bio/db/pileup.rb +221 -172
- data/lib/bio/db/sam.rb +639 -589
- data/lib/bio/db/sam/{faidx.rb → faidx_old.rb} +0 -0
- data/lib/bio/db/vcf.rb +69 -68
- data/test/.gitignore +1 -0
- data/test/{test_basic.rb → old_test_basic.rb} +33 -1
- data/test/samples/small/dupes.bam +0 -0
- data/test/samples/small/dupes.sam +274 -0
- data/test/samples/small/map_for_reheader.sam +8 -0
- data/test/samples/small/map_to_merge1.bam +0 -0
- data/test/samples/small/map_to_merge1.bam.bai +0 -0
- data/test/samples/small/map_to_merge1.sam +8 -0
- data/test/samples/small/map_to_merge2.bam +0 -0
- data/test/samples/small/map_to_merge2.bam.bai +0 -0
- data/test/samples/small/map_to_merge2.sam +8 -0
- data/test/samples/small/no_md.sam +8 -0
- data/test/samples/small/test_chr.fasta.1.bt2 +0 -0
- data/test/samples/small/test_chr.fasta.2.bt2 +0 -0
- data/test/samples/small/test_chr.fasta.3.bt2 +0 -0
- data/test/samples/small/test_chr.fasta.4.bt2 +0 -0
- data/test/samples/small/test_chr.fasta.rev.1.bt2 +0 -0
- data/test/samples/small/test_chr.fasta.rev.2.bt2 +0 -0
- data/test/samples/small/test_cov.svg +273 -0
- data/test/samples/small/testu.bam.bai +0 -0
- data/test/svg +133 -0
- data/test/test_pileup.rb +84 -0
- data/test/test_sam.rb +331 -0
- data/test/test_vcf.rb +11 -0
- data/{doc → tutorial}/tutorial.html +0 -0
- data/{doc → tutorial}/tutorial.pdf +0 -0
- metadata +56 -114
- data/doc/Bio/DB/SAM/Tools/Bam1CoreT.html +0 -159
- data/doc/Bio/DB/SAM/Tools/Bam1T.html +0 -220
- data/doc/Bio/DB/SAM/Tools/BamHeaderT.html +0 -249
- data/doc/Bio/DB/SAM/Tools/BamPileup1T.html +0 -159
- data/doc/Bio/DB/SAM/Tools/SamfileT.html +0 -171
- data/doc/Bio/DB/SAM/Tools/SamfileTX.html +0 -159
- data/doc/Bio/DB/SAMException.html +0 -205
- data/doc/LibC.html +0 -155
- data/doc/Pileup.html +0 -571
- data/doc/Vcf.html +0 -473
- data/doc/basic_styles.css +0 -31
- data/doc/classes/Bio.html +0 -139
- data/doc/classes/Bio/DB.html +0 -137
- data/doc/classes/Bio/DB/Alignment.html +0 -441
- data/doc/classes/Bio/DB/Alignment.src/M000012.html +0 -19
- data/doc/classes/Bio/DB/Alignment.src/M000013.html +0 -27
- data/doc/classes/Bio/DB/Alignment.src/M000014.html +0 -45
- data/doc/classes/Bio/DB/Alignment.src/M000015.html +0 -40
- data/doc/classes/Bio/DB/SAM.html +0 -510
- data/doc/classes/Bio/DB/SAM/Library.html +0 -135
- data/doc/classes/Bio/DB/SAM/Library.src/M000006.html +0 -28
- data/doc/classes/Bio/DB/SAM/Tools.html +0 -278
- data/doc/classes/Bio/DB/SAM/Tools.src/M000007.html +0 -20
- data/doc/classes/Bio/DB/SAM/Tools/Bam1CoreT.html +0 -111
- data/doc/classes/Bio/DB/SAM/Tools/Bam1T.html +0 -150
- data/doc/classes/Bio/DB/SAM/Tools/Bam1T.src/M000010.html +0 -20
- data/doc/classes/Bio/DB/SAM/Tools/BamHeaderT.html +0 -169
- data/doc/classes/Bio/DB/SAM/Tools/BamHeaderT.src/M000008.html +0 -19
- data/doc/classes/Bio/DB/SAM/Tools/BamHeaderT.src/M000009.html +0 -18
- data/doc/classes/Bio/DB/SAM/Tools/BamPileup1T.html +0 -111
- data/doc/classes/Bio/DB/SAM/Tools/SamfileT.html +0 -129
- data/doc/classes/Bio/DB/SAM/Tools/SamfileTX.html +0 -111
- data/doc/classes/Bio/DB/SAMException.html +0 -140
- data/doc/classes/Bio/DB/SAMException.src/M000016.html +0 -18
- data/doc/classes/Bio/DB/Sam.src/M000017.html +0 -43
- data/doc/classes/Bio/DB/Sam.src/M000018.html +0 -42
- data/doc/classes/Bio/DB/Sam.src/M000019.html +0 -18
- data/doc/classes/Bio/DB/Sam.src/M000020.html +0 -22
- data/doc/classes/Bio/DB/Sam.src/M000021.html +0 -19
- data/doc/classes/Bio/DB/Sam.src/M000022.html +0 -25
- data/doc/classes/Bio/DB/Sam.src/M000023.html +0 -28
- data/doc/classes/Bio/DB/Sam.src/M000024.html +0 -28
- data/doc/classes/Bio/DB/Sam.src/M000025.html +0 -46
- data/doc/classes/Bio/DB/Sam.src/M000026.html +0 -24
- data/doc/classes/Bio/DB/Sam.src/M000027.html +0 -19
- data/doc/classes/Bio/DB/Sam.src/M000028.html +0 -24
- data/doc/classes/Bio/DB/Sam.src/M000029.html +0 -41
- data/doc/classes/Bio/DB/Sam.src/M000030.html +0 -31
- data/doc/classes/Bio/DB/Sam.src/M000031.html +0 -86
- data/doc/classes/Bio/DB/Sam.src/M000032.html +0 -34
- data/doc/classes/Bio/DB/Tag.html +0 -160
- data/doc/classes/Bio/DB/Tag.src/M000011.html +0 -21
- data/doc/classes/LibC.html +0 -105
- data/doc/classes/Pileup.html +0 -374
- data/doc/classes/Pileup.src/M000001.html +0 -34
- data/doc/classes/Pileup.src/M000002.html +0 -21
- data/doc/classes/Pileup.src/M000003.html +0 -21
- data/doc/classes/Pileup.src/M000004.html +0 -21
- data/doc/classes/Pileup.src/M000005.html +0 -31
- data/doc/files/lib/bio-samtools_rb.html +0 -109
- data/doc/files/lib/bio/db/sam/bam_rb.html +0 -108
- data/doc/files/lib/bio/db/sam/faidx_rb.html +0 -108
- data/doc/files/lib/bio/db/sam/library_rb.html +0 -101
- data/doc/files/lib/bio/db/sam/pileup_rb.html +0 -178
- data/doc/files/lib/bio/db/sam/sam_rb.html +0 -113
- data/doc/files/lib/bio/db/sam_rb.html +0 -111
- data/doc/fr_class_index.html +0 -43
- data/doc/fr_file_index.html +0 -33
- data/doc/fr_method_index.html +0 -58
- data/doc/lib/bio-samtools_rb.html +0 -115
- data/doc/lib/bio/db/pileup_rb.html +0 -171
- data/doc/lib/bio/db/sam/bam_rb.html +0 -121
- data/doc/lib/bio/db/sam/faidx_rb.html +0 -117
- data/doc/lib/bio/db/sam/library_rb.html +0 -115
- data/doc/lib/bio/db/sam/pileup_rb.html +0 -171
- data/doc/lib/bio/db/sam/sam_rb.html +0 -121
- data/doc/lib/bio/db/sam/vcf_rb.html +0 -124
- data/doc/lib/bio/db/sam_rb.html +0 -115
- data/doc/lib/bio/db/vcf_rb.html +0 -124
- data/doc/rdoc-style.css +0 -208
- data/lib/bio/db/sam/bam.rb +0 -210
- data/lib/bio/db/sam/sam.rb +0 -86
- data/test/samples/pipe_char/test.bam +0 -0
- data/test/samples/pipe_char/test.bam.bai +0 -0
- data/test/samples/pipe_char/test.tam +0 -10
- data/test/samples/pipe_char/test_chr.fasta +0 -1000
- data/test/samples/pipe_char/test_chr.fasta.fai +0 -1
- data/test/samples/small/test +0 -0
- data/test/samples/small/test.bam +0 -0
- data/test/samples/small/test.fa +0 -20
- data/test/samples/small/test.fai +0 -0
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<div class="method-heading">
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<span class="method-name">parse_line</span><span
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class="method-args">(line, sample_names=nil)</span>
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<span class="method-click-advice">click to toggle source</span>
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@@ -386,52 +350,53 @@ GT:HQ 0|0:10,10 0|0:10,10 0/1:3,3”)</p>
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<p>gets the info in the <a href="Vcf.html">Vcf</a> lines and parses it,
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setting the attributes</p>
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<div class="method-source-code" id="parse_line-source">
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<pre><span class="ruby-comment"># File lib/bio/db/vcf.rb, line
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<pre><span class="ruby-comment"># File lib/bio/db/vcf.rb, line 28</span>
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<span class="ruby-keyword">def</span> <span class="ruby-identifier">parse_line</span>(<span class="ruby-identifier">line</span>, <span class="ruby-identifier">sample_names</span>=<span class="ruby-keyword">nil</span>)
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<span class="ruby-keyword">return</span> <span class="ruby-keyword">false</span> <span class="ruby-keyword">if</span> <span class="ruby-identifier">line</span>[<span class="ruby-value">0</span>,<span class="ruby-value">1</span>] <span class="ruby-operator">==</span> <span class="ruby-string"
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<span class="ruby-keyword">return</span> <span class="ruby-keyword">false</span> <span class="ruby-keyword">if</span> <span class="ruby-identifier">line</span>[<span class="ruby-value">0</span>,<span class="ruby-value">1</span>] <span class="ruby-operator">==</span> <span class="ruby-string">'#'</span>
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<span class="ruby-identifier">f</span> = <span class="ruby-identifier">line</span>.<span class="ruby-identifier">chomp</span>.<span class="ruby-identifier">split</span>(<span class="ruby-string">"\t"</span>, <span class="ruby-value">-1</span>)
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<span class="ruby-identifier">raise</span> <span class="ruby-string">"VCF lines must have at least 8 fields"</span> <span class="ruby-keyword">if</span> <span class="ruby-identifier">f</span>.<span class="ruby-identifier">size</span> <span class="ruby-operator"><</span> <span class="ruby-value">8</span>
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<span class="ruby-ivar">@chrom</span> = <span class="ruby-identifier">f</span>[<span class="ruby-value">0</span>]
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<span class="ruby-ivar">@pos</span> = <span class="ruby-identifier">f</span>[<span class="ruby-value">1</span>].<span class="ruby-identifier">to_i</span>
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<span class="ruby-ivar">@id</span> = <span class="ruby-string"
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<span class="ruby-ivar">@id</span> = <span class="ruby-string">'.'</span> <span class="ruby-operator">==</span> <span class="ruby-identifier">f</span>[<span class="ruby-value">2</span>] <span class="ruby-operator">?</span> <span class="ruby-keyword">nil</span> <span class="ruby-operator">:</span> <span class="ruby-identifier">f</span>[<span class="ruby-value">2</span>]
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<span class="ruby-ivar">@ref</span> = <span class="ruby-identifier">f</span>[<span class="ruby-value">3</span>]
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<span class="ruby-ivar">@alt</span> = <span class="ruby-identifier">f</span>[<span class="ruby-value">4</span>]
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<span class="ruby-ivar">@qual</span> = <span class="ruby-identifier">f</span>[<span class="ruby-value">5</span>].<span class="ruby-identifier">to_f</span>
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<span class="ruby-ivar">@filter</span> = <span class="ruby-string"
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<span class="ruby-ivar">@info</span> = <span class="ruby-string"
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<span class="ruby-ivar">@filter</span> = <span class="ruby-string">'.'</span> <span class="ruby-operator">==</span> <span class="ruby-identifier">f</span>[<span class="ruby-value">6</span>] <span class="ruby-operator">?</span> <span class="ruby-keyword">nil</span> <span class="ruby-operator">:</span> <span class="ruby-identifier">f</span>[<span class="ruby-value">6</span>]
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<span class="ruby-ivar">@info</span> = <span class="ruby-string">'.'</span> <span class="ruby-operator">==</span> <span class="ruby-identifier">f</span>[<span class="ruby-value">7</span>] <span class="ruby-operator">?</span> <span class="ruby-keyword">nil</span> <span class="ruby-operator">:</span> {}
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<span class="ruby-keyword">if</span> <span class="ruby-ivar">@info</span>
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<span class="ruby-identifier">info_vec</span> = <span class="ruby-identifier">f</span>[<span class="ruby-value">7</span>].<span class="ruby-identifier">split</span>(<span class="ruby-string">";"</span>)
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<span class="ruby-identifier">info_vec</span>.<span class="ruby-identifier">each</span> <span class="ruby-keyword">do</span> <span class="ruby-operator">|</span><span class="ruby-identifier">x</span><span class="ruby-operator">|</span>
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<span class="ruby-identifier">keyval</span> = <span class="ruby-identifier">x</span>.<span class="ruby-identifier">split</span>(<span class="ruby-string">"="</span>, <span class="ruby-value">-1</span>)
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<span class="ruby-keyword">if</span> <span class="ruby-identifier">keyval</span>.<span class="ruby-identifier">size</span> <span class="ruby-operator">==</span> <span class="ruby-value">2</span> <span class="ruby-comment"># If it
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<span class="ruby-keyword">if</span> <span class="ruby-identifier">keyval</span>.<span class="ruby-identifier">size</span> <span class="ruby-operator">==</span> <span class="ruby-value">2</span> <span class="ruby-comment"># If it's key=value</span>
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<span class="ruby-ivar">@info</span>[<span class="ruby-identifier">keyval</span>[<span class="ruby-value">0</span>]] = <span class="ruby-identifier">keyval</span>[<span class="ruby-value">1</span>]
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<span class="ruby-keyword">else</span> <span class="ruby-comment"># Otherwise, it
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<span class="ruby-keyword">else</span> <span class="ruby-comment"># Otherwise, it's just a flag</span>
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<span class="ruby-ivar">@info</span>[<span class="ruby-identifier">x</span>] = <span class="ruby-keyword">nil</span>
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<span class="ruby-keyword">end</span>
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<span class="ruby-keyword">end</span>
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<span class="ruby-keyword">end</span>
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<span class="ruby-ivar">@samples</span> = {}
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<span class="ruby-keyword">return</span> <span class="ruby-keyword">true</span> <span class="ruby-keyword">if</span> <span class="ruby-identifier">f</span>.<span class="ruby-identifier">size</span> <span class="ruby-operator">==</span> <span class="ruby-value">8</span> <span class="ruby-comment"># Has just upto info</span>
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<span class="ruby-identifier">raise</span> <span class="ruby-string">"Can
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<span class="ruby-identifier">raise</span> <span class="ruby-string">"Can't have format with no samples"</span> <span class="ruby-keyword">if</span> <span class="ruby-identifier">f</span>.<span class="ruby-identifier">size</span> <span class="ruby-operator">==</span> <span class="ruby-value">9</span>
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<span class="ruby-ivar">@format</span> = <span class="ruby-identifier">f</span>[<span class="ruby-value">8</span>]
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<span class="ruby-identifier">sample_keys</span> = <span class="ruby-ivar">@format</span>.<span class="ruby-identifier">split</span>(<span class="ruby-string">":"</span>)
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<span class="ruby-identifier">num_samples</span> = <span class="ruby-identifier">f</span>.<span class="ruby-identifier">size</span> <span class="ruby-operator">-</span> <span class="ruby-value">9</span> <span class="ruby-comment"># How many fields are past the format</span>
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<span class="ruby-keyword">if</span> <span class="ruby-identifier">sample_names</span> <span class="ruby-operator">==</span> <span class="ruby-keyword">nil</span> <span class="ruby-comment"># Make the sample names just ["1", "2", ... , "num_samples}"</span>
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<span class="ruby-identifier">sample_names</span> = (<span class="ruby-value">1</span><span class="ruby-operator">..</span><span class="ruby-identifier">num_samples</span>).<span class="ruby-identifier">to_a</span>.<span class="ruby-identifier">map</span>{<span class="ruby-operator">|</span><span class="ruby-identifier">i</span><span class="ruby-operator">|</span> <span class="ruby-identifier">i</span>.<span class="ruby-identifier">to_s</span>}
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<span class="ruby-keyword">elsif</span> <span class="ruby-identifier">sample_names</span>.<span class="ruby-identifier">size</span> <span class="ruby-operator">!=</span> <span class="ruby-identifier">num_samples</span>
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<span class="ruby-identifier">raise</span> <span class="ruby-node">"Unexpected number of samples (#{num_samples}) based on the provided sample names (#{sample_names.inspect})"</span>
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<span class="ruby-keyword">end</span>
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<span class="ruby-identifier">sample_names</span>.<span class="ruby-identifier">each_with_index</span> <span class="ruby-keyword">do</span> <span class="ruby-operator">|</span><span class="ruby-identifier">sample_name</span>, <span class="ruby-identifier">sample_index</span><span class="ruby-operator">|</span>
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<span class="ruby-identifier">i</span> = <span class="ruby-identifier">sample_index</span> <span class="ruby-operator">+</span> <span class="ruby-value">9</span> <span class="ruby-comment"># index into columns (f)</span>
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<span class="ruby-identifier">sample_values</span> = <span class="ruby-identifier">f</span>[<span class="ruby-identifier">i</span>].<span class="ruby-identifier">split</span>(<span class="ruby-string">":"</span>)
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<span class="ruby-keyword">return</span> <span class="ruby-keyword">true</span>;
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<span class="ruby-keyword">end</span></pre>
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</section>
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</div><!-- documentation -->
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</section>
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</main>
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<footer id="validator-badges">
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<p><a href="http://validator.w3.org/check/referer">
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<p>Generated by <a href="
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<p><a href="http://validator.w3.org/check/referer">Validate</a>
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<p>Generated by <a href="http://rdoc.rubyforge.org">RDoc</a> 4.1.1.
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<p>Based on <a href="http://deveiate.org/projects/Darkfish-Rdoc/">Darkfish</a> by <a href="http://deveiate.org">Michael Granger</a>.
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</footer>
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