bio-samtools 0.6.2 → 2.0.3
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- checksums.yaml +4 -4
- data/Gemfile +3 -2
- data/README.md +4 -7
- data/VERSION +1 -1
- data/bio-samtools.gemspec +47 -105
- data/doc/Bio.html +68 -131
- data/doc/Bio/DB.html +51 -111
- data/doc/Bio/DB/Alignment.html +135 -363
- data/doc/Bio/DB/Pileup.html +183 -170
- data/doc/Bio/DB/SAM.html +1396 -820
- data/doc/Bio/DB/SAM/Library.html +73 -123
- data/doc/Bio/DB/SAM/Tools.html +51 -273
- data/doc/Bio/DB/Tag.html +78 -124
- data/doc/Bio/DB/Vcf.html +111 -147
- data/doc/LICENSE_txt.html +113 -148
- data/doc/created.rid +9 -10
- data/doc/fonts.css +167 -0
- data/doc/fonts/Lato-Light.ttf +0 -0
- data/doc/fonts/Lato-LightItalic.ttf +0 -0
- data/doc/fonts/Lato-Regular.ttf +0 -0
- data/doc/fonts/Lato-RegularItalic.ttf +0 -0
- data/doc/fonts/SourceCodePro-Bold.ttf +0 -0
- data/doc/fonts/SourceCodePro-Regular.ttf +0 -0
- data/doc/images/add.png +0 -0
- data/doc/images/arrow_up.png +0 -0
- data/doc/images/delete.png +0 -0
- data/doc/images/tag_blue.png +0 -0
- data/doc/index.html +48 -54
- data/doc/js/darkfish.js +9 -22
- data/doc/js/search.js +20 -5
- data/doc/js/search_index.js +1 -1
- data/doc/rdoc.css +255 -218
- data/doc/table_of_contents.html +256 -137
- data/ext/Rakefile +57 -0
- data/lib/bio-samtools.rb +7 -2
- data/lib/bio/BIOExtensions.rb +89 -0
- data/lib/bio/db/alignment.rb +59 -0
- data/lib/bio/db/fastadb.rb +255 -0
- data/lib/bio/db/pileup.rb +221 -172
- data/lib/bio/db/sam.rb +639 -589
- data/lib/bio/db/sam/{faidx.rb → faidx_old.rb} +0 -0
- data/lib/bio/db/vcf.rb +69 -68
- data/test/.gitignore +1 -0
- data/test/{test_basic.rb → old_test_basic.rb} +33 -1
- data/test/samples/small/dupes.bam +0 -0
- data/test/samples/small/dupes.sam +274 -0
- data/test/samples/small/map_for_reheader.sam +8 -0
- data/test/samples/small/map_to_merge1.bam +0 -0
- data/test/samples/small/map_to_merge1.bam.bai +0 -0
- data/test/samples/small/map_to_merge1.sam +8 -0
- data/test/samples/small/map_to_merge2.bam +0 -0
- data/test/samples/small/map_to_merge2.bam.bai +0 -0
- data/test/samples/small/map_to_merge2.sam +8 -0
- data/test/samples/small/no_md.sam +8 -0
- data/test/samples/small/test_chr.fasta.1.bt2 +0 -0
- data/test/samples/small/test_chr.fasta.2.bt2 +0 -0
- data/test/samples/small/test_chr.fasta.3.bt2 +0 -0
- data/test/samples/small/test_chr.fasta.4.bt2 +0 -0
- data/test/samples/small/test_chr.fasta.rev.1.bt2 +0 -0
- data/test/samples/small/test_chr.fasta.rev.2.bt2 +0 -0
- data/test/samples/small/test_cov.svg +273 -0
- data/test/samples/small/testu.bam.bai +0 -0
- data/test/svg +133 -0
- data/test/test_pileup.rb +84 -0
- data/test/test_sam.rb +331 -0
- data/test/test_vcf.rb +11 -0
- data/{doc → tutorial}/tutorial.html +0 -0
- data/{doc → tutorial}/tutorial.pdf +0 -0
- metadata +56 -114
- data/doc/Bio/DB/SAM/Tools/Bam1CoreT.html +0 -159
- data/doc/Bio/DB/SAM/Tools/Bam1T.html +0 -220
- data/doc/Bio/DB/SAM/Tools/BamHeaderT.html +0 -249
- data/doc/Bio/DB/SAM/Tools/BamPileup1T.html +0 -159
- data/doc/Bio/DB/SAM/Tools/SamfileT.html +0 -171
- data/doc/Bio/DB/SAM/Tools/SamfileTX.html +0 -159
- data/doc/Bio/DB/SAMException.html +0 -205
- data/doc/LibC.html +0 -155
- data/doc/Pileup.html +0 -571
- data/doc/Vcf.html +0 -473
- data/doc/basic_styles.css +0 -31
- data/doc/classes/Bio.html +0 -139
- data/doc/classes/Bio/DB.html +0 -137
- data/doc/classes/Bio/DB/Alignment.html +0 -441
- data/doc/classes/Bio/DB/Alignment.src/M000012.html +0 -19
- data/doc/classes/Bio/DB/Alignment.src/M000013.html +0 -27
- data/doc/classes/Bio/DB/Alignment.src/M000014.html +0 -45
- data/doc/classes/Bio/DB/Alignment.src/M000015.html +0 -40
- data/doc/classes/Bio/DB/SAM.html +0 -510
- data/doc/classes/Bio/DB/SAM/Library.html +0 -135
- data/doc/classes/Bio/DB/SAM/Library.src/M000006.html +0 -28
- data/doc/classes/Bio/DB/SAM/Tools.html +0 -278
- data/doc/classes/Bio/DB/SAM/Tools.src/M000007.html +0 -20
- data/doc/classes/Bio/DB/SAM/Tools/Bam1CoreT.html +0 -111
- data/doc/classes/Bio/DB/SAM/Tools/Bam1T.html +0 -150
- data/doc/classes/Bio/DB/SAM/Tools/Bam1T.src/M000010.html +0 -20
- data/doc/classes/Bio/DB/SAM/Tools/BamHeaderT.html +0 -169
- data/doc/classes/Bio/DB/SAM/Tools/BamHeaderT.src/M000008.html +0 -19
- data/doc/classes/Bio/DB/SAM/Tools/BamHeaderT.src/M000009.html +0 -18
- data/doc/classes/Bio/DB/SAM/Tools/BamPileup1T.html +0 -111
- data/doc/classes/Bio/DB/SAM/Tools/SamfileT.html +0 -129
- data/doc/classes/Bio/DB/SAM/Tools/SamfileTX.html +0 -111
- data/doc/classes/Bio/DB/SAMException.html +0 -140
- data/doc/classes/Bio/DB/SAMException.src/M000016.html +0 -18
- data/doc/classes/Bio/DB/Sam.src/M000017.html +0 -43
- data/doc/classes/Bio/DB/Sam.src/M000018.html +0 -42
- data/doc/classes/Bio/DB/Sam.src/M000019.html +0 -18
- data/doc/classes/Bio/DB/Sam.src/M000020.html +0 -22
- data/doc/classes/Bio/DB/Sam.src/M000021.html +0 -19
- data/doc/classes/Bio/DB/Sam.src/M000022.html +0 -25
- data/doc/classes/Bio/DB/Sam.src/M000023.html +0 -28
- data/doc/classes/Bio/DB/Sam.src/M000024.html +0 -28
- data/doc/classes/Bio/DB/Sam.src/M000025.html +0 -46
- data/doc/classes/Bio/DB/Sam.src/M000026.html +0 -24
- data/doc/classes/Bio/DB/Sam.src/M000027.html +0 -19
- data/doc/classes/Bio/DB/Sam.src/M000028.html +0 -24
- data/doc/classes/Bio/DB/Sam.src/M000029.html +0 -41
- data/doc/classes/Bio/DB/Sam.src/M000030.html +0 -31
- data/doc/classes/Bio/DB/Sam.src/M000031.html +0 -86
- data/doc/classes/Bio/DB/Sam.src/M000032.html +0 -34
- data/doc/classes/Bio/DB/Tag.html +0 -160
- data/doc/classes/Bio/DB/Tag.src/M000011.html +0 -21
- data/doc/classes/LibC.html +0 -105
- data/doc/classes/Pileup.html +0 -374
- data/doc/classes/Pileup.src/M000001.html +0 -34
- data/doc/classes/Pileup.src/M000002.html +0 -21
- data/doc/classes/Pileup.src/M000003.html +0 -21
- data/doc/classes/Pileup.src/M000004.html +0 -21
- data/doc/classes/Pileup.src/M000005.html +0 -31
- data/doc/files/lib/bio-samtools_rb.html +0 -109
- data/doc/files/lib/bio/db/sam/bam_rb.html +0 -108
- data/doc/files/lib/bio/db/sam/faidx_rb.html +0 -108
- data/doc/files/lib/bio/db/sam/library_rb.html +0 -101
- data/doc/files/lib/bio/db/sam/pileup_rb.html +0 -178
- data/doc/files/lib/bio/db/sam/sam_rb.html +0 -113
- data/doc/files/lib/bio/db/sam_rb.html +0 -111
- data/doc/fr_class_index.html +0 -43
- data/doc/fr_file_index.html +0 -33
- data/doc/fr_method_index.html +0 -58
- data/doc/lib/bio-samtools_rb.html +0 -115
- data/doc/lib/bio/db/pileup_rb.html +0 -171
- data/doc/lib/bio/db/sam/bam_rb.html +0 -121
- data/doc/lib/bio/db/sam/faidx_rb.html +0 -117
- data/doc/lib/bio/db/sam/library_rb.html +0 -115
- data/doc/lib/bio/db/sam/pileup_rb.html +0 -171
- data/doc/lib/bio/db/sam/sam_rb.html +0 -121
- data/doc/lib/bio/db/sam/vcf_rb.html +0 -124
- data/doc/lib/bio/db/sam_rb.html +0 -115
- data/doc/lib/bio/db/vcf_rb.html +0 -124
- data/doc/rdoc-style.css +0 -208
- data/lib/bio/db/sam/bam.rb +0 -210
- data/lib/bio/db/sam/sam.rb +0 -86
- data/test/samples/pipe_char/test.bam +0 -0
- data/test/samples/pipe_char/test.bam.bai +0 -0
- data/test/samples/pipe_char/test.tam +0 -10
- data/test/samples/pipe_char/test_chr.fasta +0 -1000
- data/test/samples/pipe_char/test_chr.fasta.fai +0 -1
- data/test/samples/small/test +0 -0
- data/test/samples/small/test.bam +0 -0
- data/test/samples/small/test.fa +0 -20
- data/test/samples/small/test.fai +0 -0
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<span class="ruby-constant">LibC</span>.<span class="ruby-identifier">free</span> <span class="ruby-identifier">object_id</span>.<span class="ruby-identifier">sam</span>
|
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<span class="ruby-keyword">end</span></pre>
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<section id="public-class-5Buntitled-5D-method-details" class="method-section">
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<header>
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<h3>Public Class Methods</h3>
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|
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|
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|
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<pre><span class="ruby-comment"># File lib/bio/db/alignment.rb, line 24</span>
|
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|
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<span class="ruby-keyword">def</span> <span class="ruby-identifier">initialize</span>(<span class="ruby-identifier">sam_string</span>)
|
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|
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|
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|
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|
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|
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|
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|
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|
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|
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<span class="ruby-ivar">@cigar</span> = <span class="ruby-identifier">s</span>[<span class="ruby-value">5</span>]
|
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|
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|
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|
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+
<span class="ruby-ivar">@isize</span> = <span class="ruby-identifier">s</span>[<span class="ruby-value">8</span>].<span class="ruby-identifier">to_i</span>
|
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|
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<span class="ruby-ivar">@seq</span> = <span class="ruby-identifier">s</span>[<span class="ruby-value">9</span>]
|
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|
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<span class="ruby-ivar">@tags</span> = {}
|
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|
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|
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|
-
<span class="ruby-identifier">t</span> = <span class="ruby-constant">Tag</span>.<span class="ruby-identifier">new</span>
|
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|
+
<span class="ruby-identifier">t</span> = <span class="ruby-constant">Bio</span><span class="ruby-operator">::</span><span class="ruby-constant">DB</span><span class="ruby-operator">::</span><span class="ruby-constant">Tag</span>.<span class="ruby-identifier">new</span>
|
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|
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|
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|
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}
|
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|
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</div><!-- sam-3D-method -->
|
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|
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|
-
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|
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|
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|
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|
-
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|
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|
-
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|
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|
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<span class="method-click-advice">click to toggle source</span>
|
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|
-
</div>
|
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|
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|
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|
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<pre><span class="ruby-comment"># File lib/bio/db/sam.rb, line 582</span>
|
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|
-
<span class="ruby-keyword">def</span> <span class="ruby-identifier">set</span>(<span class="ruby-identifier">bam_alignment</span>, <span class="ruby-identifier">header</span>)
|
729
|
-
<span class="ruby-comment">#Create the FFI object</span>
|
730
|
-
<span class="ruby-ivar">@al</span> = <span class="ruby-constant">Bio</span><span class="ruby-operator">::</span><span class="ruby-constant">DB</span><span class="ruby-operator">::</span><span class="ruby-constant">SAM</span><span class="ruby-operator">::</span><span class="ruby-constant">Tools</span><span class="ruby-operator">::</span><span class="ruby-constant">Bam1T</span>.<span class="ruby-identifier">new</span>(<span class="ruby-identifier">bam_alignment</span>)
|
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|
-
|
732
|
-
<span class="ruby-comment">#set the raw data</span>
|
733
|
-
<span class="ruby-identifier">tmp_str</span> = <span class="ruby-constant">Bio</span><span class="ruby-operator">::</span><span class="ruby-constant">DB</span><span class="ruby-operator">::</span><span class="ruby-constant">SAM</span><span class="ruby-operator">::</span><span class="ruby-constant">Tools</span>.<span class="ruby-identifier">bam_format1</span>(<span class="ruby-identifier">header</span>,<span class="ruby-identifier">al</span>)
|
734
|
-
<span class="ruby-comment">#self.sam = tmp_str</span>
|
735
|
-
<span class="ruby-comment">#ObjectSpace.define_finalizer(self, proc {|id| puts "Finalizer one on #{id}" })</span>
|
736
|
-
<span class="ruby-keyword">self</span>.<span class="ruby-identifier">sam</span> = <span class="ruby-constant">String</span>.<span class="ruby-identifier">new</span>(<span class="ruby-identifier">tmp_str</span>)
|
737
|
-
<span class="ruby-comment">#LibC.free tmp_str</span>
|
738
|
-
<span class="ruby-comment">#Set values calculated by libbam</span>
|
739
|
-
<span class="ruby-identifier">core</span> = <span class="ruby-identifier">al</span>[<span class="ruby-value">:core</span>]
|
740
|
-
<span class="ruby-identifier">cigar</span> = <span class="ruby-identifier">al</span>[<span class="ruby-value">:data</span>][<span class="ruby-identifier">core</span>[<span class="ruby-value">:l_qname</span>]]<span class="ruby-comment">#define bam1_cigar(b) ((uint32_t*)((b)->data + (b)->core.l_qname)) </span>
|
741
|
-
<span class="ruby-ivar">@calend</span> = <span class="ruby-constant">Bio</span><span class="ruby-operator">::</span><span class="ruby-constant">DB</span><span class="ruby-operator">::</span><span class="ruby-constant">SAM</span><span class="ruby-operator">::</span><span class="ruby-constant">Tools</span>.<span class="ruby-identifier">bam_calend</span>(<span class="ruby-identifier">core</span>,<span class="ruby-identifier">cigar</span>)
|
742
|
-
<span class="ruby-ivar">@qlen</span> = <span class="ruby-constant">Bio</span><span class="ruby-operator">::</span><span class="ruby-constant">DB</span><span class="ruby-operator">::</span><span class="ruby-constant">SAM</span><span class="ruby-operator">::</span><span class="ruby-constant">Tools</span>.<span class="ruby-identifier">bam_cigar2qlen</span>(<span class="ruby-identifier">core</span>,<span class="ruby-identifier">cigar</span>)
|
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|
-
|
744
|
-
<span class="ruby-comment">#process the flags</span>
|
745
|
-
<span class="ruby-ivar">@is_paired</span> = <span class="ruby-ivar">@flag</span> & <span class="ruby-value">0x0001</span> <span class="ruby-operator">></span> <span class="ruby-value">0</span>
|
746
|
-
<span class="ruby-ivar">@is_mapped</span> = <span class="ruby-ivar">@flag</span> & <span class="ruby-value">0x0002</span> <span class="ruby-operator">></span> <span class="ruby-value">0</span>
|
747
|
-
<span class="ruby-ivar">@query_unmapped</span> = <span class="ruby-ivar">@flag</span> & <span class="ruby-value">0x0004</span> <span class="ruby-operator">></span> <span class="ruby-value">0</span>
|
748
|
-
<span class="ruby-ivar">@mate_unmapped</span> = <span class="ruby-ivar">@flag</span> & <span class="ruby-value">0x0008</span> <span class="ruby-operator">></span> <span class="ruby-value">0</span>
|
749
|
-
<span class="ruby-ivar">@query_strand</span> = <span class="ruby-operator">!</span>(<span class="ruby-ivar">@flag</span> & <span class="ruby-value">0x0010</span> <span class="ruby-operator">></span> <span class="ruby-value">0</span>)
|
750
|
-
<span class="ruby-ivar">@mate_strand</span> = <span class="ruby-operator">!</span>(<span class="ruby-ivar">@flag</span> & <span class="ruby-value">0x0020</span> <span class="ruby-operator">></span> <span class="ruby-value">0</span>)
|
751
|
-
<span class="ruby-ivar">@first_in_pair</span> = <span class="ruby-ivar">@flag</span> & <span class="ruby-value">0x0040</span> <span class="ruby-operator">></span> <span class="ruby-value">0</span>
|
752
|
-
<span class="ruby-ivar">@second_in_pair</span> = <span class="ruby-ivar">@flag</span> & <span class="ruby-value">0x0080</span> <span class="ruby-operator">></span> <span class="ruby-value">0</span>
|
753
|
-
<span class="ruby-ivar">@primary</span> = <span class="ruby-operator">!</span>(<span class="ruby-ivar">@flag</span> & <span class="ruby-value">0x0100</span> <span class="ruby-operator">></span> <span class="ruby-value">0</span>)
|
754
|
-
<span class="ruby-ivar">@failed_quality</span> = <span class="ruby-ivar">@flag</span> & <span class="ruby-value">0x0200</span> <span class="ruby-operator">></span> <span class="ruby-value">0</span>
|
755
|
-
<span class="ruby-ivar">@is_duplicate</span> = <span class="ruby-ivar">@flag</span> & <span class="ruby-value">0x0400</span> <span class="ruby-operator">></span> <span class="ruby-value">0</span>
|
516
|
+
}
|
517
|
+
|
518
|
+
<span class="ruby-ivar">@is_paired</span> = (<span class="ruby-ivar">@flag</span> <span class="ruby-operator">&</span> <span class="ruby-value">0x0001</span>) <span class="ruby-operator">></span> <span class="ruby-value">0</span>
|
519
|
+
<span class="ruby-ivar">@is_mapped</span> = <span class="ruby-ivar">@flag</span> <span class="ruby-operator">&</span> <span class="ruby-value">0x0002</span> <span class="ruby-operator">></span> <span class="ruby-value">0</span>
|
520
|
+
<span class="ruby-ivar">@query_unmapped</span> = <span class="ruby-ivar">@flag</span> <span class="ruby-operator">&</span> <span class="ruby-value">0x0004</span> <span class="ruby-operator">></span> <span class="ruby-value">0</span>
|
521
|
+
<span class="ruby-ivar">@mate_unmapped</span> = <span class="ruby-ivar">@flag</span> <span class="ruby-operator">&</span> <span class="ruby-value">0x0008</span> <span class="ruby-operator">></span> <span class="ruby-value">0</span>
|
522
|
+
<span class="ruby-ivar">@query_strand</span> = <span class="ruby-operator">!</span>(<span class="ruby-ivar">@flag</span> <span class="ruby-operator">&</span> <span class="ruby-value">0x0010</span> <span class="ruby-operator">></span> <span class="ruby-value">0</span>)
|
523
|
+
<span class="ruby-ivar">@mate_strand</span> = <span class="ruby-operator">!</span>(<span class="ruby-ivar">@flag</span> <span class="ruby-operator">&</span> <span class="ruby-value">0x0020</span> <span class="ruby-operator">></span> <span class="ruby-value">0</span>)
|
524
|
+
<span class="ruby-ivar">@first_in_pair</span> = <span class="ruby-ivar">@flag</span> <span class="ruby-operator">&</span> <span class="ruby-value">0x0040</span> <span class="ruby-operator">></span> <span class="ruby-value">0</span>
|
525
|
+
<span class="ruby-ivar">@second_in_pair</span> = <span class="ruby-ivar">@flag</span> <span class="ruby-operator">&</span> <span class="ruby-value">0x0080</span> <span class="ruby-operator">></span> <span class="ruby-value">0</span>
|
526
|
+
<span class="ruby-ivar">@primary</span> = <span class="ruby-operator">!</span>(<span class="ruby-ivar">@flag</span> <span class="ruby-operator">&</span> <span class="ruby-value">0x0100</span> <span class="ruby-operator">></span> <span class="ruby-value">0</span>)
|
527
|
+
<span class="ruby-ivar">@failed_quality</span> = <span class="ruby-ivar">@flag</span> <span class="ruby-operator">&</span> <span class="ruby-value">0x0200</span> <span class="ruby-operator">></span> <span class="ruby-value">0</span>
|
528
|
+
<span class="ruby-ivar">@is_duplicate</span> = <span class="ruby-ivar">@flag</span> <span class="ruby-operator">&</span> <span class="ruby-value">0x0400</span> <span class="ruby-operator">></span> <span class="ruby-value">0</span>
|
756
529
|
|
757
530
|
<span class="ruby-keyword">end</span></pre>
|
758
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-
</div
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531
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+
</div>
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759
532
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760
533
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</div>
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761
534
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762
535
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763
536
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764
537
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765
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-
</div
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538
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+
</div>
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766
539
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767
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768
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</section
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541
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</section>
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769
542
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770
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</section
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771
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-
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772
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</div><!-- documentation -->
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543
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</section>
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544
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+
</main>
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775
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<footer id="validator-badges">
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776
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<p><a href="http://validator.w3.org/check/referer">
|
777
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-
<p>Generated by <a href="
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778
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-
<p>
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547
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+
<footer id="validator-badges" role="contentinfo">
|
548
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+
<p><a href="http://validator.w3.org/check/referer">Validate</a>
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549
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+
<p>Generated by <a href="http://rdoc.rubyforge.org">RDoc</a> 4.1.1.
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550
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+
<p>Based on <a href="http://deveiate.org/projects/Darkfish-Rdoc/">Darkfish</a> by <a href="http://deveiate.org">Michael Granger</a>.
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</footer>
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