bio-samtools-wrapper 2.7.0
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- checksums.yaml +7 -0
- data/.document +5 -0
- data/.travis.yml +27 -0
- data/Gemfile +20 -0
- data/LICENSE.txt +702 -0
- data/README.md +501 -0
- data/Rakefile +73 -0
- data/VERSION +1 -0
- data/bin/bam_consensus.rb +85 -0
- data/bio-samtools-wrapper.gemspec +181 -0
- data/doc/Bio/DB/Alignment.html +552 -0
- data/doc/Bio/DB/Pileup.html +711 -0
- data/doc/Bio/DB/SAM/Library.html +167 -0
- data/doc/Bio/DB/SAM/Tools.html +109 -0
- data/doc/Bio/DB/SAM.html +1853 -0
- data/doc/Bio/DB/Tag.html +208 -0
- data/doc/Bio/DB/Vcf.html +431 -0
- data/doc/Bio/DB.html +105 -0
- data/doc/Bio.html +175 -0
- data/doc/LICENSE_txt.html +846 -0
- data/doc/created.rid +9 -0
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- data/doc/index.html +106 -0
- data/doc/js/darkfish.js +140 -0
- data/doc/js/jquery.js +18 -0
- data/doc/js/navigation.js +142 -0
- data/doc/js/search.js +109 -0
- data/doc/js/search_index.js +1 -0
- data/doc/js/searcher.js +228 -0
- data/doc/rdoc.css +580 -0
- data/doc/table_of_contents.html +305 -0
- data/ext/Makefile-bioruby.patch +12 -0
- data/ext/Makefile-suse.patch +11 -0
- data/ext/mkrf_conf.rb +118 -0
- data/lib/bio/BIOExtensions.rb +89 -0
- data/lib/bio/db/alignment.rb +64 -0
- data/lib/bio/db/fastadb.rb +320 -0
- data/lib/bio/db/pileup.rb +273 -0
- data/lib/bio/db/sam/external/COPYING +21 -0
- data/lib/bio/db/sam/external/VERSION +1 -0
- data/lib/bio/db/sam/library.rb +32 -0
- data/lib/bio/db/sam.rb +778 -0
- data/lib/bio/db/vcf.rb +105 -0
- data/lib/bio-samtools-wrapper.rb +9 -0
- data/test/.gitignore +1 -0
- data/test/helper.rb +18 -0
- data/test/sample.vcf +24 -0
- data/test/samples/.gitignore +1 -0
- data/test/samples/LCI/NC_001988.ffn +2 -0
- data/test/samples/LCI/test.bam +0 -0
- data/test/samples/LCI/test.bam.bai +0 -0
- data/test/samples/small/dupes.bam +0 -0
- data/test/samples/small/dupes.sam +274 -0
- data/test/samples/small/ids2.txt +1 -0
- data/test/samples/small/map_for_reheader.sam +8 -0
- data/test/samples/small/map_to_merge1.bam +0 -0
- data/test/samples/small/map_to_merge1.bam.bai +0 -0
- data/test/samples/small/map_to_merge1.sam +8 -0
- data/test/samples/small/map_to_merge2.bam +0 -0
- data/test/samples/small/map_to_merge2.bam.bai +0 -0
- data/test/samples/small/map_to_merge2.sam +8 -0
- data/test/samples/small/no_md.sam +8 -0
- data/test/samples/small/sorted.bam +0 -0
- data/test/samples/small/sorted.bam.bai +0 -0
- data/test/samples/small/test.sai +0 -0
- data/test/samples/small/test.tam +10 -0
- data/test/samples/small/test_chr.fasta +1000 -0
- data/test/samples/small/test_chr.fasta.1.bt2 +0 -0
- data/test/samples/small/test_chr.fasta.2.bt2 +0 -0
- data/test/samples/small/test_chr.fasta.3.bt2 +0 -0
- data/test/samples/small/test_chr.fasta.4.bt2 +0 -0
- data/test/samples/small/test_chr.fasta.amb +2 -0
- data/test/samples/small/test_chr.fasta.ann +3 -0
- data/test/samples/small/test_chr.fasta.bwt +0 -0
- data/test/samples/small/test_chr.fasta.pac +0 -0
- data/test/samples/small/test_chr.fasta.rbwt +0 -0
- data/test/samples/small/test_chr.fasta.rev.1.bt2 +0 -0
- data/test/samples/small/test_chr.fasta.rev.2.bt2 +0 -0
- data/test/samples/small/test_chr.fasta.rpac +0 -0
- data/test/samples/small/test_chr.fasta.rsa +0 -0
- data/test/samples/small/test_chr.fasta.sa +0 -0
- data/test/samples/small/test_cov.svg +273 -0
- data/test/samples/small/test_fastadb.fasta +34 -0
- data/test/samples/small/testu.bam +0 -0
- data/test/samples/small/testu.bed +2 -0
- data/test/test_bio-samtools-wrapper.rb +1 -0
- data/test/test_fastadb.rb +89 -0
- data/test/test_pileup.rb +90 -0
- data/test/test_sam.rb +421 -0
- data/test/test_vcf.rb +79 -0
- data/tutorial/tutorial.html +474 -0
- data/tutorial/tutorial.md +424 -0
- data/tutorial/tutorial.pdf +0 -0
- metadata +254 -0
data/doc/Bio/DB/Tag.html
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<p>a class to represent the SAM OPT values, presented in SAM as
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<p>Generated by <a href="http://rdoc.rubyforge.org">RDoc</a> 4.1.1.
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<p>Based on <a href="http://deveiate.org/projects/Darkfish-Rdoc/">Darkfish</a> by <a href="http://deveiate.org">Michael Granger</a>.
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<h3>Public Class Methods</h3>
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class="method-args">(line=nil, sample_names=nil)</span>
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<p>create the vcf object, use the ordered list of sample names to label
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samples if provided ['A', 'B', 'C'], otherwise
|
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uses, 1,2,3 etc</p>
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<pre>vcf = Bio::DB::Vcf("19 111 . A C 9.6 . . GT:HQ 0|0:10,10 0|0:10,10 0/1:3,3")</pre>
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<pre><span class="ruby-comment"># File lib/bio/db/vcf.rb, line 15</span>
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<span class="ruby-keyword">def</span> <span class="ruby-identifier">initialize</span>(<span class="ruby-identifier">line</span>=<span class="ruby-keyword">nil</span>, <span class="ruby-identifier">sample_names</span>=<span class="ruby-keyword">nil</span>)
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<span class="ruby-ivar">@info</span> = {}
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<span class="ruby-ivar">@samples</span> = {}
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<span class="ruby-identifier">parse_line</span>(<span class="ruby-identifier">line</span>, <span class="ruby-identifier">sample_names</span>) <span class="ruby-keyword">if</span> <span class="ruby-identifier">line</span> <span class="ruby-operator">!=</span> <span class="ruby-keyword">nil</span>
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class="method-args">(x)</span>
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<p>tests if the current variable is an Integer</p>
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<pre><span class="ruby-comment"># File lib/bio/db/vcf.rb, line 23</span>
|
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<span class="ruby-keyword">def</span> <span class="ruby-identifier">int_or_raw</span>(<span class="ruby-identifier">x</span>)
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<span class="ruby-constant">Integer</span>.<span class="ruby-identifier">new</span>(<span class="ruby-identifier">x</span>) <span class="ruby-keyword">rescue</span> <span class="ruby-identifier">x</span>
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<span class="ruby-keyword">end</span></pre>
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<p>gets the info in the <a href="Vcf.html">Vcf</a> lines and parses it,
|
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setting the attributes</p>
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<pre><span class="ruby-comment"># File lib/bio/db/vcf.rb, line 28</span>
|
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<span class="ruby-keyword">def</span> <span class="ruby-identifier">parse_line</span>(<span class="ruby-identifier">line</span>, <span class="ruby-identifier">sample_names</span>=<span class="ruby-keyword">nil</span>)
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<span class="ruby-keyword">return</span> <span class="ruby-keyword">false</span> <span class="ruby-keyword">if</span> <span class="ruby-identifier">line</span>[<span class="ruby-value">0</span>,<span class="ruby-value">1</span>] <span class="ruby-operator">==</span> <span class="ruby-string">'#'</span>
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<span class="ruby-identifier">f</span> = <span class="ruby-identifier">line</span>.<span class="ruby-identifier">chomp</span>.<span class="ruby-identifier">split</span>(<span class="ruby-string">"\t"</span>, <span class="ruby-value">-1</span>)
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<span class="ruby-identifier">raise</span> <span class="ruby-string">"VCF lines must have at least 8 fields"</span> <span class="ruby-keyword">if</span> <span class="ruby-identifier">f</span>.<span class="ruby-identifier">size</span> <span class="ruby-operator"><</span> <span class="ruby-value">8</span>
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<span class="ruby-ivar">@chrom</span> = <span class="ruby-identifier">f</span>[<span class="ruby-value">0</span>]
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<span class="ruby-ivar">@pos</span> = <span class="ruby-identifier">f</span>[<span class="ruby-value">1</span>].<span class="ruby-identifier">to_i</span>
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<span class="ruby-ivar">@id</span> = <span class="ruby-string">'.'</span> <span class="ruby-operator">==</span> <span class="ruby-identifier">f</span>[<span class="ruby-value">2</span>] <span class="ruby-operator">?</span> <span class="ruby-keyword">nil</span> <span class="ruby-operator">:</span> <span class="ruby-identifier">f</span>[<span class="ruby-value">2</span>]
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<span class="ruby-ivar">@ref</span> = <span class="ruby-identifier">f</span>[<span class="ruby-value">3</span>]
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<span class="ruby-ivar">@alt</span> = <span class="ruby-identifier">f</span>[<span class="ruby-value">4</span>]
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<span class="ruby-ivar">@qual</span> = <span class="ruby-identifier">f</span>[<span class="ruby-value">5</span>].<span class="ruby-identifier">to_f</span>
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<span class="ruby-ivar">@filter</span> = <span class="ruby-string">'.'</span> <span class="ruby-operator">==</span> <span class="ruby-identifier">f</span>[<span class="ruby-value">6</span>] <span class="ruby-operator">?</span> <span class="ruby-keyword">nil</span> <span class="ruby-operator">:</span> <span class="ruby-identifier">f</span>[<span class="ruby-value">6</span>]
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<span class="ruby-ivar">@info</span> = <span class="ruby-string">'.'</span> <span class="ruby-operator">==</span> <span class="ruby-identifier">f</span>[<span class="ruby-value">7</span>] <span class="ruby-operator">?</span> <span class="ruby-keyword">nil</span> <span class="ruby-operator">:</span> {}
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<span class="ruby-keyword">if</span> <span class="ruby-ivar">@info</span>
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<span class="ruby-identifier">info_vec</span> = <span class="ruby-identifier">f</span>[<span class="ruby-value">7</span>].<span class="ruby-identifier">split</span>(<span class="ruby-string">";"</span>)
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<span class="ruby-identifier">info_vec</span>.<span class="ruby-identifier">each</span> <span class="ruby-keyword">do</span> <span class="ruby-operator">|</span><span class="ruby-identifier">x</span><span class="ruby-operator">|</span>
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<span class="ruby-identifier">keyval</span> = <span class="ruby-identifier">x</span>.<span class="ruby-identifier">split</span>(<span class="ruby-string">"="</span>, <span class="ruby-value">-1</span>)
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<span class="ruby-keyword">if</span> <span class="ruby-identifier">keyval</span>.<span class="ruby-identifier">size</span> <span class="ruby-operator">==</span> <span class="ruby-value">2</span> <span class="ruby-comment"># If it's key=value</span>
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<span class="ruby-ivar">@info</span>[<span class="ruby-identifier">keyval</span>[<span class="ruby-value">0</span>]] = <span class="ruby-identifier">keyval</span>[<span class="ruby-value">1</span>]
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<span class="ruby-keyword">else</span> <span class="ruby-comment"># Otherwise, it's just a flag</span>
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<span class="ruby-ivar">@info</span>[<span class="ruby-identifier">x</span>] = <span class="ruby-keyword">nil</span>
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<span class="ruby-keyword">end</span>
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<span class="ruby-keyword">end</span>
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<span class="ruby-keyword">end</span>
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<span class="ruby-ivar">@samples</span> = {}
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<span class="ruby-keyword">return</span> <span class="ruby-keyword">true</span> <span class="ruby-keyword">if</span> <span class="ruby-identifier">f</span>.<span class="ruby-identifier">size</span> <span class="ruby-operator">==</span> <span class="ruby-value">8</span> <span class="ruby-comment"># Has just upto info</span>
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<span class="ruby-identifier">raise</span> <span class="ruby-string">"Can't have format with no samples"</span> <span class="ruby-keyword">if</span> <span class="ruby-identifier">f</span>.<span class="ruby-identifier">size</span> <span class="ruby-operator">==</span> <span class="ruby-value">9</span>
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<span class="ruby-ivar">@format</span> = <span class="ruby-identifier">f</span>[<span class="ruby-value">8</span>]
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<span class="ruby-identifier">sample_keys</span> = <span class="ruby-ivar">@format</span>.<span class="ruby-identifier">split</span>(<span class="ruby-string">":"</span>)
|
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<span class="ruby-identifier">num_samples</span> = <span class="ruby-identifier">f</span>.<span class="ruby-identifier">size</span> <span class="ruby-operator">-</span> <span class="ruby-value">9</span> <span class="ruby-comment"># How many fields are past the format</span>
|
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<span class="ruby-keyword">if</span> <span class="ruby-identifier">sample_names</span> <span class="ruby-operator">==</span> <span class="ruby-keyword">nil</span> <span class="ruby-comment"># Make the sample names just ["1", "2", ... , "num_samples}"</span>
|
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<span class="ruby-identifier">sample_names</span> = (<span class="ruby-value">1</span><span class="ruby-operator">..</span><span class="ruby-identifier">num_samples</span>).<span class="ruby-identifier">to_a</span>.<span class="ruby-identifier">map</span>{<span class="ruby-operator">|</span><span class="ruby-identifier">i</span><span class="ruby-operator">|</span> <span class="ruby-identifier">i</span>.<span class="ruby-identifier">to_s</span>}
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<span class="ruby-keyword">elsif</span> <span class="ruby-identifier">sample_names</span>.<span class="ruby-identifier">size</span> <span class="ruby-operator">!=</span> <span class="ruby-identifier">num_samples</span>
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<span class="ruby-identifier">raise</span> <span class="ruby-node">"Unexpected number of samples (#{num_samples}) based on the provided sample names (#{sample_names.inspect})"</span>
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<span class="ruby-keyword">end</span>
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<span class="ruby-identifier">sample_names</span>.<span class="ruby-identifier">each_with_index</span> <span class="ruby-keyword">do</span> <span class="ruby-operator">|</span><span class="ruby-identifier">sample_name</span>, <span class="ruby-identifier">sample_index</span><span class="ruby-operator">|</span>
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<span class="ruby-identifier">i</span> = <span class="ruby-identifier">sample_index</span> <span class="ruby-operator">+</span> <span class="ruby-value">9</span> <span class="ruby-comment"># index into columns (f)</span>
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<span class="ruby-identifier">sample_values</span> = <span class="ruby-identifier">f</span>[<span class="ruby-identifier">i</span>].<span class="ruby-identifier">split</span>(<span class="ruby-string">":"</span>)
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<span class="ruby-identifier">raise</span> <span class="ruby-node">"Expected number of sample values to be <= number of sample keys in FORMAT column Format=#{@format} but sample=#{f[i]}"</span> <span class="ruby-keyword">if</span> <span class="ruby-identifier">sample_values</span>.<span class="ruby-identifier">size</span> <span class="ruby-operator">></span> <span class="ruby-identifier">sample_keys</span>.<span class="ruby-identifier">size</span>
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<span class="ruby-ivar">@samples</span>[<span class="ruby-identifier">sample_name</span>] = {}
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<span class="ruby-identifier">sample_keys</span>.<span class="ruby-identifier">each_with_index</span> {<span class="ruby-operator">|</span><span class="ruby-identifier">key</span>, <span class="ruby-identifier">value_index</span><span class="ruby-operator">|</span> <span class="ruby-ivar">@samples</span>[<span class="ruby-identifier">sample_name</span>][<span class="ruby-identifier">key</span>] = <span class="ruby-identifier">sample_values</span>[<span class="ruby-identifier">value_index</span>] <span class="ruby-operator">||</span> <span class="ruby-string">""</span>}
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<span class="ruby-keyword">end</span>
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<span class="ruby-keyword">return</span> <span class="ruby-keyword">true</span>;
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<span class="ruby-keyword">end</span></pre>
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<footer id="validator-badges" role="contentinfo">
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<p><a href="http://validator.w3.org/check/referer">Validate</a>
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<p>Generated by <a href="http://rdoc.rubyforge.org">RDoc</a> 4.1.1.
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<p>Based on <a href="http://deveiate.org/projects/Darkfish-Rdoc/">Darkfish</a> by <a href="http://deveiate.org">Michael Granger</a>.
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</footer>
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