bio-samtools-wrapper 2.7.0
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- checksums.yaml +7 -0
- data/.document +5 -0
- data/.travis.yml +27 -0
- data/Gemfile +20 -0
- data/LICENSE.txt +702 -0
- data/README.md +501 -0
- data/Rakefile +73 -0
- data/VERSION +1 -0
- data/bin/bam_consensus.rb +85 -0
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- data/ext/Makefile-bioruby.patch +12 -0
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- data/lib/bio/BIOExtensions.rb +89 -0
- data/lib/bio/db/alignment.rb +64 -0
- data/lib/bio/db/fastadb.rb +320 -0
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- data/lib/bio/db/sam/external/COPYING +21 -0
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- data/lib/bio/db/sam.rb +778 -0
- data/lib/bio/db/vcf.rb +105 -0
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- data/test/.gitignore +1 -0
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|
+
AAGAAGCTTATGCCTTCTAAGCCAACAACCCTCAAGGATATGAGCAAGTGGCGTTCCTTCTTTGAAAACT
|
9
|
+
GGAACTTGTACATGAGTCAGTGTCGCGGTGCTGCGGCTATCCCTCTTTCGTACGTTTACCGTACCAACGA
|
10
|
+
GCAGCCCGAGACCGCTTTGGTCGGAACCTATGTGAATATGGATGCCTATTTGGTTGCCCAGACAGTACTG
|
11
|
+
TCTGGTTCCAACTTTGAGATCGATAATCAATGGGTTTTTGACGAATTCAAGGAAGCAATCACTACAACCG
|
12
|
+
GACCTGGTTGGTCTTTCATCAAGACGTACAACCGAAGCAAGGACGGTCGTGCTGCCATTTTGAAATTAAA
|
13
|
+
GGAACAGGCGGAAGGAACATTAAACGAGTCCGTTCGCCGTGATGATGCCATCAAGATCCTGTCAACTACG
|
14
|
+
>chr.2
|
15
|
+
ACATACAATGGTCCGAGTTGTAACTGGAATATTGATATGCTGTTGCAGAAATTTCAGTATGCCATCTCGG
|
16
|
+
AATTGGTCGAAATTGACGGAGTCGCGTTGCCGGATGGGCAGCTTGTGACTTATTTGGTCCAGGCATTGAA
|
17
|
+
GCGCAGCTTTTTGTGAAGACTTTTGTGTCTTCGTCCACGAGCAAATCCGAAAACACGCCTCGACAGGTCA
|
18
|
+
ATGATGTGCAAACATCAGGTAGTGGGGCCTCCGGTGGGAGTAAGAAAGGAGGTACCGGGAAAGGAGCCAG
|
19
|
+
CAAGCAGACTCCCTTCAAGGGTGCAGTCACGGCTCGCAGTTATACTCCGGGAGAATGGAAAAGATTGTCC
|
20
|
+
AAGGACCAACAGGAAAAAGTGCGATCGCTGCGTAATAAAAAGAAGCAAGGAGGGAAACCCGAGGAATCAG
|
21
|
+
AGAGGAGTGTTGACAGTGTAGCACGGGATGAGCCTGTGGACACTAAGGAAGTCCATACCAGCAGTGATAT
|
22
|
+
GGAACCGACTTCAGATGCGGCTGGCCTGCAATTTGGCCGTGGTGCGTATAAGAAATCGGTCGGATTCACT
|
23
|
+
GCGGACACCGCTTCTCCTTCAGAAAACGGAACGAAGAAGCAGAAAACGCATCACGATGCGTGAAACGCGG
|
24
|
+
CACCCAATGCCAGTGTTTCGGGGACTAAGCAATGCATTTTACCAGATCGAGTGATATTGAGCCTCACCTC
|
25
|
+
TACACGCAGCATTTGTGATCTCAACGCATGCACTCATCTTGGTGAGGGCCGCTGCGAGTTGGATTCACAT
|
26
|
+
>chr_3
|
27
|
+
GCAGACACATGCGTGGCTGGGGCAAACACTGTCTTGATTGGTGAATCGCAGAAGTCCGTAACTGTGCG
|
28
|
+
CTTTCTCCGGTGAATATTCTGCACTGAAGAATATCCCCATTGGAACGGTTGCCACAGCTTACACAGCC
|
29
|
+
AGAAGACGGGAGAGTGGTGCTTCTTATTATTAATCAGGCCCTATTCTTTGGGGACAGATTGAAACACC
|
30
|
+
CTAGACCCCCAACCAGATGCGAGACTTTGGCATTGAAGTTGACGATGCCCCTCGGCAGTACGTCGCCA
|
31
|
+
ACCAAGCACTCTTTGTATGTTCCTGACTCCCAACTTCGGATTCCGCTGCAGCTGCGCGGTATATTCTC
|
32
|
+
GTTTTTGGAGTCGGAAGCCCACGCAACAGGAACTTGACGAGTGTGAGCATATCATACTCACCTCTGAT
|
33
|
+
GTGCCGTGGGACTTGCTCAACGGACTTTGCCCGTCGAGAAGAAGAGGCCGCTAAGAGAGACCGGAGCG
|
34
|
+
TATCATTGACACAACGGGACTTTCCACTGGCCACGCAATCCTATCAGCACACCCATATGGTATACG
|
Binary file
|
@@ -0,0 +1 @@
|
|
1
|
+
require 'helper'
|
@@ -0,0 +1,89 @@
|
|
1
|
+
$: << File.expand_path(File.dirname(__FILE__) + '/../lib')
|
2
|
+
$: << File.expand_path('.')
|
3
|
+
|
4
|
+
require 'rubygems'
|
5
|
+
require 'bio/db/sam'
|
6
|
+
require "test/unit"
|
7
|
+
#gem 'ruby-prof'
|
8
|
+
gem 'test-unit'
|
9
|
+
#require "ruby-prof"
|
10
|
+
|
11
|
+
|
12
|
+
class TestBioDbfastaDB < Test::Unit::TestCase
|
13
|
+
|
14
|
+
def setup
|
15
|
+
@test_folder = "test/samples/small"
|
16
|
+
@testReference = @test_folder + "/test_fastadb.fasta"
|
17
|
+
@fasta_local = Bio::DB::Fasta::FastaFile.new(
|
18
|
+
fasta: @testReference,
|
19
|
+
samtools: false
|
20
|
+
)
|
21
|
+
|
22
|
+
@fasta_samtools = Bio::DB::Fasta::FastaFile.new(
|
23
|
+
:fasta => @testReference,
|
24
|
+
samtools: true
|
25
|
+
)
|
26
|
+
end
|
27
|
+
class << self
|
28
|
+
def shutdown
|
29
|
+
File.delete("test/samples/small/test_fastadb.fasta.fai")
|
30
|
+
end
|
31
|
+
end
|
32
|
+
|
33
|
+
def test_faidx
|
34
|
+
@fasta_samtools.index()
|
35
|
+
test_fai_file = @testReference+".fai"
|
36
|
+
#test that the .fai file exists
|
37
|
+
assert_nothing_thrown do
|
38
|
+
File.open(test_fai_file, "r")
|
39
|
+
end
|
40
|
+
#test that the file is not empty
|
41
|
+
assert(File.size(test_fai_file) > 0, "From test_faidx: .fai file is empty")
|
42
|
+
end
|
43
|
+
|
44
|
+
|
45
|
+
|
46
|
+
def test_fetch_reference
|
47
|
+
#this is the first 70 nucleotides of the test seqeunce
|
48
|
+
@fasta_samtools.faidx()
|
49
|
+
|
50
|
+
test_regions = { "chr_1:1-70" => "CCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTA",
|
51
|
+
"chr_1:68-74" => "CTAACCC",
|
52
|
+
"chr.2:300-450" => "GGTGCAGTCACGGCTCGCAGTTATACTCCGGGAGAATGGAAAAGATTGTCCAAGGACCAA\
|
53
|
+
CAGGAAAAAGTGCGATCGCTGCGTAATAAAAAGAAGCAAGGAG\
|
54
|
+
GGAAACCCGAGGAATCAGAGAGGAGTGTTGACAGTGTAGCACGGGATG",
|
55
|
+
"chr_3:536-542" => "GTATACG",
|
56
|
+
"chr.2:765-770" => "TCACAT"
|
57
|
+
}
|
58
|
+
|
59
|
+
test_regions.each_pair do |region, seq_expected|
|
60
|
+
seq_fetched = @fasta_samtools.fetch_sequence(region)
|
61
|
+
assert_equal(seq_expected, seq_fetched.upcase)
|
62
|
+
|
63
|
+
seq_fetched_local = @fasta_local.fetch_sequence(region)
|
64
|
+
assert_equal(seq_expected, seq_fetched_local.upcase)
|
65
|
+
end
|
66
|
+
|
67
|
+
test_regions = {
|
68
|
+
"chr_3:772-780" => Bio::DB::Fasta::FastaDBException,
|
69
|
+
"chr_3:-1-10" => Bio::DB::Fasta::FastaDBException,
|
70
|
+
"chr_3:0-10" => Bio::DB::Fasta::FastaDBException,
|
71
|
+
"bfafdaads" => Bio::DB::Fasta::FastaDBException,
|
72
|
+
"chr.2:765-771" => Bio::DB::Fasta::FastaDBException,
|
73
|
+
"chr_2:765-770" => Bio::DB::Fasta::FastaDBException,
|
74
|
+
|
75
|
+
}
|
76
|
+
|
77
|
+
test_regions.each_pair do |region, expected_eception|
|
78
|
+
assert_raise expected_eception do
|
79
|
+
#seq_fetched =
|
80
|
+
@fasta_samtools.fetch_sequence(region)
|
81
|
+
end
|
82
|
+
|
83
|
+
assert_raise expected_eception do
|
84
|
+
#seq_fetched_local =
|
85
|
+
@fasta_local.fetch_sequence(region)
|
86
|
+
end
|
87
|
+
end
|
88
|
+
end
|
89
|
+
end
|