bento_search 1.3.0 → 1.4.2
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- checksums.yaml +4 -4
- data/README.md +42 -2
- data/app/controllers/bento_search/search_controller.rb +1 -1
- data/app/models/bento_search/author.rb +6 -4
- data/app/models/bento_search/link.rb +8 -5
- data/app/models/bento_search/multi_searcher.rb +1 -1
- data/app/models/bento_search/registrar.rb +2 -1
- data/app/models/bento_search/result_item.rb +37 -21
- data/app/models/bento_search/results/serialization.rb +136 -0
- data/app/models/bento_search/results.rb +46 -1
- data/app/models/bento_search/search_engine.rb +22 -17
- data/app/search_engines/bento_search/eds_engine.rb +1 -1
- data/app/search_engines/bento_search/journal_tocs_for_journal.rb +21 -3
- data/lib/bento_search/version.rb +1 -1
- data/lib/bento_search.rb +5 -2
- data/test/search_engines/journal_tocs_for_journal_test.rb +117 -93
- data/test/search_engines/search_engine_test.rb +1 -1
- data/test/unit/serialization_test.rb +249 -0
- data/test/vcr_cassettes/journal_tocs/empty_results_on_bad_ISSN.yml +19 -25
- data/test/vcr_cassettes/journal_tocs/error_on_bad_registered_email.yml +19 -26
- data/test/vcr_cassettes/journal_tocs/error_on_error_response.yml +33 -37
- data/test/vcr_cassettes/journal_tocs/fetch_xml_with_hits.yml +308 -305
- data/test/vcr_cassettes/journal_tocs/fills_out_metadata.yml +515 -377
- data/test/vcr_cassettes/journal_tocs/smoke_test.yml +308 -305
- data/test/vcr_cassettes/journal_tocs/sorts_by_date.yml +922 -0
- metadata +7 -6
- data/test/dummy/db/test.sqlite3 +0 -0
- data/test/dummy/log/test.log +0 -164426
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method: get
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uri: http://www.journaltocs.ac.uk/api/journals/0026-2617?output=articles&user=nobody@example.com
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- Wed, 02 Sep 2015 20:57:46 GMT
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\r\n xmlns:prism=\"http://prismstandard.org/namespaces/1.2/basic/\"
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\r\n\t\t\t\t xmlns:dc=\"http://purl.org/dc/elements/1.1/\" \r\n\t\t\t\t xmlns:mn=\"http://usefulinc.com/rss/manifest/\"\r\n\t\t\t\t
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xmlns:content=\"http://purl.org/rss/1.0/modules/content/\" \r\n\t\t\t\t xmlns=\"http://purl.org/rss/1.0/\">\n\n
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\ <channel rdf:about=\"http://www.journaltocs.hw.ac.uk/api/journals\">\r\n
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\ <title>JournalTOCs API - Microbiology (20 articles)</title>\r\n <link>http://www.journaltocs.ac.uk/api/journals/0026-2617</link>\r\n
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\ <description><![CDATA[Your query: 0026-2617 has returned 20 articles.
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They are listed in alphabetical order per journal (maximum number of returned
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items is 3000).]]></description>\r\n <dc:publisher>JournalTOCs API</dc:publisher>\r\n
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\ <dc:creator>JOURNALTOCS API PROJECT</dc:creator>\r\n\t\t<dc:coverage>1</dc:coverage>\r\n
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\ <image rdf:resource=\"http://www.journaltocs.ac.uk/images/jtocslogo.gif\"
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/>\r\n <items>\r\n <rdf:Seq><rdf:li rdf:resource=\"http://link.springer.com/10.1134/S0026261715040128\"
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/>\n<rdf:li rdf:resource=\"http://link.springer.com/10.1134/S0026261715040098\"
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/>\n<rdf:li rdf:resource=\"http://link.springer.com/10.1134/S0026261715040165\"
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/>\n<rdf:li rdf:resource=\"http://link.springer.com/10.1134/S0026261715040086\"
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/>\n<rdf:li rdf:resource=\"http://link.springer.com/10.1134/S0026261715040153\"
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/>\n<rdf:li rdf:resource=\"http://link.springer.com/10.1134/S0026261715030157\"
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/>\n<rdf:li rdf:resource=\"http://link.springer.com/10.1134/S0026261715030030\"
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/>\n<rdf:li rdf:resource=\"http://link.springer.com/10.1134/S0026261715040074\"
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/>\n<rdf:li rdf:resource=\"http://link.springer.com/10.1134/S0026261715040141\"
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/>\n<rdf:li rdf:resource=\"http://link.springer.com/10.1134/S0026261715040104\"
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/>\n<rdf:li rdf:resource=\"http://link.springer.com/10.1134/S0026261715040062\"
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/>\n<rdf:li rdf:resource=\"http://link.springer.com/10.1134/S0026261715040025\"
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/>\n<rdf:li rdf:resource=\"http://link.springer.com/10.1134/S0026261715040037\"
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/>\n<rdf:li rdf:resource=\"http://link.springer.com/10.1134/S0026261715040189\"
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/>\n<rdf:li rdf:resource=\"http://link.springer.com/10.1134/S0026261715030194\"
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/>\n<rdf:li rdf:resource=\"http://link.springer.com/10.1134/S0026261715040177\"
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/>\n<rdf:li rdf:resource=\"http://link.springer.com/10.1134/S0026261715040050\"
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/>\n<rdf:li rdf:resource=\"http://link.springer.com/10.1134/S0026261715040116\"
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/>\n<rdf:li rdf:resource=\"http://link.springer.com/10.1134/S0026261715040049\"
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/>\n\r\n </rdf:Seq>\r\n </items>\r\n </channel>\r\n <item rdf:about=\"http://link.springer.com/10.1134/S0026261715040128\">\n<title>Structure
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of the archaeal community in the Black Sea photic zone</title>\r\n<link>http://link.springer.com/10.1134/S0026261715040128</link>\r\n<description><h3
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class=&quot;a-plus-plus&quot;>Abstract</h3>\n <p
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class=&quot;a-plus-plus&quot;>Qualitative and quantitative analysis
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of the structure of the archaeal community of the photic zone of the Black
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Sea water column was carried out. Real-time PCR revealed 2 × 10<sup
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class=&quot;a-plus-plus&quot;>4</sup> archaeal cells/mL (4.2%
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of the total cell number) at a 15-m depth. The structure of archaeal communities
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in the subsurface water column was investigated using the sequencing by synthesis
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technology (Illumina/Solexa) of the 16S rRNA genes. The Marine Group II phylogenetic
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cluster belonging to the phylum <em class=&quot;a-plus-plus&quot;>Euryarchaeota</em>
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was the most numerous archaeal group (1.2–1.7 × 10<sup class=&quot;a-plus-plus&quot;>4</sup>
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cells/mL). The Marine Group I phylogenetic cluster (phylum <em class=&quot;a-plus-plus&quot;>Thaumarchaeota</em>)
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was the second most numerous group (40% of the free-living archaea or 7.7
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× 10<sup class=&quot;a-plus-plus&quot;>3</sup> cells/mL).
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Sequences of the <em class=&quot;a-plus-plus&quot;>‘Nitrosopumilus’</em>
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cluster were revealed among Marine Group I sequences due to high homology
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(over 90%). A group of archaea belonging to the Deep-sea Hydrothermal Vent
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Euryarchaeotic Group 6 (DHVEG-6) (phylum <em class=&quot;a-plus-plus&quot;>Euryarchaeota</em>)
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was also detected. The 16S rRNA gene sequences belonging to this cluster were
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revealed only in the suspension fraction. High homology level (over 90%) suggested
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classification of most DHVEG-6 sequences within the <em class=&quot;a-plus-plus&quot;>‘Parvarchaeum’</em>
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cluster. In spite of a noticeable methane peak detected at 15-m depth, no
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sequences of methanogens were found.</p></description>\r\n<dc:identifier>http://link.springer.com/10.1134/S0026261715040128</dc:identifier>\r\n<dc:date>2015-07-01</dc:date>\n<dc:publisher>Springer-Verlag</dc:publisher>\n<prism:PublicationName>Microbiology</prism:PublicationName>\n<prism:publicationDate>2015-07-01</prism:publicationDate>\n<content:encoded><![CDATA[<p><a
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href=\"http://link.springer.com/10.1134/S0026261715040128\"><b>Structure of
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the archaeal community in the Black Sea photic zone</b></A><br /> <br /><i>Microbiology,
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Vol. , No. (2015) pp. - </i><br /><h3 class=&quot;a-plus-plus&quot;>Abstract</h3>\n
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\ <p class=&quot;a-plus-plus&quot;>Qualitative
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and quantitative analysis of the structure of the archaeal community of the
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photic zone of the Black Sea water column was carried out. Real-time PCR revealed
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2 × 10<sup class=&quot;a-plus-plus&quot;>4</sup>
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archaeal cells/mL (4.2% of the total cell number) at a 15-m depth. The structure
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of archaeal communities in the subsurface water column was investigated using
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the sequencing by synthesis technology (Illumina/Solexa) of the 16S rRNA genes.
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The Marine Group II phylogenetic cluster belonging to the phylum <em class=&quot;a-plus-plus&quot;>Euryarchaeota</em>
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was the most numerous archaeal group (1.2–1.7 × 10<sup class=&quot;a-plus-plus&quot;>4</sup>
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cells/mL). The Marine Group I phylogenetic cluster (phylum <em class=&quot;a-plus-plus&quot;>Thaumarchaeota</em>)
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was the second most numerous group (40% of the free-living archaea or 7.7
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× 10<sup class=&quot;a-plus-plus&quot;>3</sup> cells/mL).
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Sequences of the <em class=&quot;a-plus-plus&quot;>‘Nitrosopumilus’</em>
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98
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cluster were revealed among Marine Group I sequences due to high homology
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(over 90%). A group of archaea belonging to the Deep-sea Hydrothermal Vent
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Euryarchaeotic Group 6 (DHVEG-6) (phylum <em class=&quot;a-plus-plus&quot;>Euryarchaeota</em>)
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was also detected. The 16S rRNA gene sequences belonging to this cluster were
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revealed only in the suspension fraction. High homology level (over 90%) suggested
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classification of most DHVEG-6 sequences within the <em class=&quot;a-plus-plus&quot;>‘Parvarchaeum’</em>
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cluster. In spite of a noticeable methane peak detected at 15-m depth, no
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sequences of methanogens were found.</p></p>]]></content:encoded>\r\n</item>\n<item
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rdf:about=\"http://link.springer.com/10.1134/S0026261715040098\">\n<title>Molecular
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identification and resistance investigation of atrazine degrading bacteria
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in the sediments of Karun River, Ahvaz, Iran</title>\r\n<link>http://link.springer.com/10.1134/S0026261715040098</link>\r\n<description><h3
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class=&quot;a-plus-plus&quot;>Abstract</h3>\n <p
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class=&quot;a-plus-plus&quot;>Nowadays, the use of pesticides as
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plant protection products has become widely prevalent, leading to the entry
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of large amounts of pesticides into soil and water resources, and subsequently,
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a threat to the environment. The objective of this study was molecular identification
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and resistance investigation of atrazine degrading bacteria in the sediments
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of Karun River, Ahvaz, Iran. Nine samples were collected in both summer (Jul)
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and autumn (Nov) year 2012 from a depth of 3 to 5 cm of the sediments. Atrazine
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degrading bacteria were enriched in a culture containing atrazine with initial
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concentration of 30 mg/L. Identification of isolated bacteria was performed
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by morphological and biochemical test and molecular analysis based on 16S
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rDNA sequencing. The atrazine biodegradation rate was obtained by high-performance
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liquid chromatography (HPLC). Six strains was identified including <em
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class=&quot;a-plus-plus&quot;>Achromobacter insolitus</em>
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strain F-N3, <em class=&quot;a-plus-plus&quot;>Delftia tsuruhatensis</em>
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strain F-N4, <em class=&quot;a-plus-plus&quot;>Klebsiella pneumonia</em>
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F-N1, <em class=&quot;a-plus-plus&quot;>Enterobacter ludwigii</em>
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strain F-N5, <em class=&quot;a-plus-plus&quot;>Serratia marcescens</em>
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strain F-N6 in both summer and autumn, and <em class=&quot;a-plus-plus&quot;>Exiguobacterium
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profundum</em> strain F-N2 only in the summer. The minimum inhibitory
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concentration (MIC) of atrazine showed that the most resistant species belonged
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to <em class=&quot;a-plus-plus&quot;>E. ludwigii</em>
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F-N5 and <em class=&quot;a-plus-plus&quot;>D. tsuruhatensis</em>
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F-N4 in the both seasons. The atrazine degradation rates of the two strains
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reached 90 and 85%, respectively after 7 days culture. Result showed that
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indigenous bacteria in the Karun River can degrade the atrazine effectively.</p></description>\r\n<dc:identifier>http://link.springer.com/10.1134/S0026261715040098</dc:identifier>\r\n<dc:date>2015-07-01</dc:date>\n<dc:publisher>Springer-Verlag</dc:publisher>\n<prism:PublicationName>Microbiology</prism:PublicationName>\n<prism:publicationDate>2015-07-01</prism:publicationDate>\n<content:encoded><![CDATA[<p><a
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href=\"http://link.springer.com/10.1134/S0026261715040098\"><b>Molecular identification
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136
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and resistance investigation of atrazine degrading bacteria in the sediments
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137
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of Karun River, Ahvaz, Iran</b></A><br /> <br /><i>Microbiology, Vol. , No.
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\ (2015) pp. - </i><br /><h3 class=&quot;a-plus-plus&quot;>Abstract</h3>\n
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\ <p class=&quot;a-plus-plus&quot;>Nowadays,
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the use of pesticides as plant protection products has become widely prevalent,
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leading to the entry of large amounts of pesticides into soil and water resources,
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and subsequently, a threat to the environment. The objective of this study
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was molecular identification and resistance investigation of atrazine degrading
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144
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bacteria in the sediments of Karun River, Ahvaz, Iran. Nine samples were collected
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145
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in both summer (Jul) and autumn (Nov) year 2012 from a depth of 3 to 5 cm
|
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146
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of the sediments. Atrazine degrading bacteria were enriched in a culture containing
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atrazine with initial concentration of 30 mg/L. Identification of isolated
|
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bacteria was performed by morphological and biochemical test and molecular
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149
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analysis based on 16S rDNA sequencing. The atrazine biodegradation rate was
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150
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obtained by high-performance liquid chromatography (HPLC). Six strains was
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identified including <em class=&quot;a-plus-plus&quot;>Achromobacter
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insolitus</em> strain F-N3, <em class=&quot;a-plus-plus&quot;>Delftia
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153
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tsuruhatensis</em> strain F-N4, <em class=&quot;a-plus-plus&quot;>Klebsiella
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pneumonia</em> F-N1, <em class=&quot;a-plus-plus&quot;>Enterobacter
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ludwigii</em> strain F-N5, <em class=&quot;a-plus-plus&quot;>Serratia
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156
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marcescens</em> strain F-N6 in both summer and autumn, and <em class=&quot;a-plus-plus&quot;>Exiguobacterium
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profundum</em> strain F-N2 only in the summer. The minimum inhibitory
|
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158
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concentration (MIC) of atrazine showed that the most resistant species belonged
|
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159
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to <em class=&quot;a-plus-plus&quot;>E. ludwigii</em>
|
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160
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F-N5 and <em class=&quot;a-plus-plus&quot;>D. tsuruhatensis</em>
|
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161
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F-N4 in the both seasons. The atrazine degradation rates of the two strains
|
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reached 90 and 85%, respectively after 7 days culture. Result showed that
|
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indigenous bacteria in the Karun River can degrade the atrazine effectively.</p></p>]]></content:encoded>\r\n</item>\n<item
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rdf:about=\"http://link.springer.com/10.1134/S0026261715040165\">\n<title>Sulfate-reducing
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165
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bacteria of the genus Desulfovibrio from south vietnam seacoast</title>\r\n<link>http://link.springer.com/10.1134/S0026261715040165</link>\r\n<description><h3
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166
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class=&quot;a-plus-plus&quot;>Abstract</h3>\n <p
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class=&quot;a-plus-plus&quot;>Nine strains of sulfate-reducing
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bacteria were isolated from a biofouling of corroded steel samples incubated
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in a marine environment near Nha Trang, South Vietnam. Sulfate-reducing bacteria
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were obtained from all samples with black corrosion products (in rust-filled
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metal cavities, beneath the <em class=&quot;a-plus-plus&quot;>Balanus</em>
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and oyster booths, and beneath <em class=&quot;a-plus-plus&quot;>Bryozoa</em>
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or algal colonies). Analysis of the 16S rRNA gene sequences of these strains
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174
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showed that they belonged to the genus <em class=&quot;a-plus-plus&quot;>Desulfovibrio</em>,
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175
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with <em class=&quot;a-plus-plus&quot;>D. salexigens</em>,
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<em class=&quot;a-plus-plus&quot;>D. marinisediminis</em>,
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<em class=&quot;a-plus-plus&quot;>D. alaskensis</em>,
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<em class=&quot;a-plus-plus&quot;>D. bizertensis D. indonesiensis</em>,
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and <em class=&quot;a-plus-plus&quot;>D. dechloracetivorans</em>
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180
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+
as the closest phylogenetic relatives (98–99% similarity). According
|
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181
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+
to the 16S rRNA gene sequencing, one <em class=&quot;a-plus-plus&quot;>Desulfovibrio</em>
|
|
182
|
+
isolate was related to “<em class=&quot;a-plus-plus&quot;>D.
|
|
183
|
+
caledoniensis”</em>, although the similarity did not exceed 97.0%.
|
|
184
|
+
All strains utilized hydrogen (in the presence of acetate and CO<sub class=&quot;a-plus-plus&quot;>2</sub>),
|
|
185
|
+
lactate, pyruvate, formate, and fumarate, but not acetate. Utilization of
|
|
186
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+
other substrates varied from strain to strain. Some isolates were capable
|
|
187
|
+
of slow autotrophic growth with H<sub class=&quot;a-plus-plus&quot;>2</sub>
|
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188
|
+
as the sole electron donor. <em class=&quot;a-plus-plus&quot;>D.
|
|
189
|
+
indonesiensis</em> and <em class=&quot;a-plus-plus&quot;>D.
|
|
190
|
+
alaskensis</em> strains were tolerant to long-term exposure to atmospheric
|
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191
|
+
oxygen exposure and could grow in the presence of 0.1% O<sub class=&quot;a-plus-plus&quot;>2</sub>
|
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+
in the gas phase.</p></description>\r\n<dc:identifier>http://link.springer.com/10.1134/S0026261715040165</dc:identifier>\r\n<dc:date>2015-07-01</dc:date>\n<dc:publisher>Springer-Verlag</dc:publisher>\n<prism:PublicationName>Microbiology</prism:PublicationName>\n<prism:publicationDate>2015-07-01</prism:publicationDate>\n<content:encoded><![CDATA[<p><a
|
|
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|
+
href=\"http://link.springer.com/10.1134/S0026261715040165\"><b>Sulfate-reducing
|
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194
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+
bacteria of the genus Desulfovibrio from south vietnam seacoast</b></A><br
|
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195
|
+
/> <br /><i>Microbiology, Vol. , No. (2015) pp. - </i><br /><h3 class=&quot;a-plus-plus&quot;>Abstract</h3>\n
|
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196
|
+
\ <p class=&quot;a-plus-plus&quot;>Nine strains
|
|
197
|
+
of sulfate-reducing bacteria were isolated from a biofouling of corroded steel
|
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198
|
+
samples incubated in a marine environment near Nha Trang, South Vietnam. Sulfate-reducing
|
|
199
|
+
bacteria were obtained from all samples with black corrosion products (in
|
|
200
|
+
rust-filled metal cavities, beneath the <em class=&quot;a-plus-plus&quot;>Balanus</em>
|
|
201
|
+
and oyster booths, and beneath <em class=&quot;a-plus-plus&quot;>Bryozoa</em>
|
|
202
|
+
or algal colonies). Analysis of the 16S rRNA gene sequences of these strains
|
|
203
|
+
showed that they belonged to the genus <em class=&quot;a-plus-plus&quot;>Desulfovibrio</em>,
|
|
204
|
+
with <em class=&quot;a-plus-plus&quot;>D. salexigens</em>,
|
|
205
|
+
<em class=&quot;a-plus-plus&quot;>D. marinisediminis</em>,
|
|
206
|
+
<em class=&quot;a-plus-plus&quot;>D. alaskensis</em>,
|
|
207
|
+
<em class=&quot;a-plus-plus&quot;>D. bizertensis D. indonesiensis</em>,
|
|
208
|
+
and <em class=&quot;a-plus-plus&quot;>D. dechloracetivorans</em>
|
|
209
|
+
as the closest phylogenetic relatives (98–99% similarity). According
|
|
210
|
+
to the 16S rRNA gene sequencing, one <em class=&quot;a-plus-plus&quot;>Desulfovibrio</em>
|
|
211
|
+
isolate was related to “<em class=&quot;a-plus-plus&quot;>D.
|
|
212
|
+
caledoniensis”</em>, although the similarity did not exceed 97.0%.
|
|
213
|
+
All strains utilized hydrogen (in the presence of acetate and CO<sub class=&quot;a-plus-plus&quot;>2</sub>),
|
|
214
|
+
lactate, pyruvate, formate, and fumarate, but not acetate. Utilization of
|
|
215
|
+
other substrates varied from strain to strain. Some isolates were capable
|
|
216
|
+
of slow autotrophic growth with H<sub class=&quot;a-plus-plus&quot;>2</sub>
|
|
217
|
+
as the sole electron donor. <em class=&quot;a-plus-plus&quot;>D.
|
|
218
|
+
indonesiensis</em> and <em class=&quot;a-plus-plus&quot;>D.
|
|
219
|
+
alaskensis</em> strains were tolerant to long-term exposure to atmospheric
|
|
220
|
+
oxygen exposure and could grow in the presence of 0.1% O<sub class=&quot;a-plus-plus&quot;>2</sub>
|
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221
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+
in the gas phase.</p></p>]]></content:encoded>\r\n</item>\n<item rdf:about=\"http://link.springer.com/10.1134/S002626171504013X\">\n<title>Occurrence,
|
|
222
|
+
diversity, and abundance of methanogenic archaea in terrestrial hot springs
|
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223
|
+
of Kamchatka and Saõ Miguel Island</title>\r\n<link>http://link.springer.com/10.1134/S002626171504013X</link>\r\n<description><h3
|
|
224
|
+
class=&quot;a-plus-plus&quot;>Abstract</h3>\n <p
|
|
225
|
+
class=&quot;a-plus-plus&quot;>Detection and analysis of the <em
|
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226
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+
class=&quot;a-plus-plus&quot;>mcrA</em> gene encoding methyl-coenzyme
|
|
227
|
+
M reductase, the key enzyme of methanogenesis, was used to assess occurrence
|
|
228
|
+
and diversity of methanogenic archaea in terrestrial hot springs of Kamchatka
|
|
229
|
+
and Sa~o Miguel Island (the Azores). For this analysis, phylogeny of methanogens
|
|
230
|
+
was initially reconstructed based on available sequences of the <em class=&quot;a-plus-plus&quot;>mcrA</em>
|
|
231
|
+
gene, which is a common functional and phylogenetic marker for this physiological
|
|
232
|
+
group of prokaryotes. Methanogens were revealed in most of the studied terrestrial
|
|
233
|
+
hot springs with temperatures from 51 to 89°C, although they constituted
|
|
234
|
+
an insignificant portion of the microbial population. The <em class=&quot;a-plus-plus&quot;>mcrA</em>
|
|
235
|
+
gene sequences revealed in the samples belonged to members of the genera <em
|
|
236
|
+
class=&quot;a-plus-plus&quot;>Methanothermobacter, Methanothermus</em>,
|
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237
|
+
and <em class=&quot;a-plus-plus&quot;>Methanothrix</em>,
|
|
238
|
+
previously detected in hot springs, as well as to methanogens not found earlier
|
|
239
|
+
in these environments. The latter belonged to <em class=&quot;a-plus-plus&quot;>Methanomassiliicoccales,
|
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240
|
+
Methanocellales</em>, and <em class=&quot;a-plus-plus&quot;>Methanomethylovorans</em>,
|
|
241
|
+
as well as to MCR-2a, the new deep phylogenetic cluster of uncultured methanogenic
|
|
242
|
+
archaea; its phylotypes were present in all springs where the <em class=&quot;a-plus-plus&quot;>mcrA</em>
|
|
243
|
+
gene was detected. Our results indicate high diversity of the thermophilic
|
|
244
|
+
methanogens inhabiting terrestrial hot springs and the presence among them
|
|
245
|
+
of new groups with yet unknown substrate specificity.</p></description>\r\n<dc:identifier>http://link.springer.com/10.1134/S002626171504013X</dc:identifier>\r\n<dc:date>2015-07-01</dc:date>\n<dc:publisher>Springer-Verlag</dc:publisher>\n<prism:PublicationName>Microbiology</prism:PublicationName>\n<prism:publicationDate>2015-07-01</prism:publicationDate>\n<content:encoded><![CDATA[<p><a
|
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246
|
+
href=\"http://link.springer.com/10.1134/S002626171504013X\"><b>Occurrence,
|
|
247
|
+
diversity, and abundance of methanogenic archaea in terrestrial hot springs
|
|
248
|
+
of Kamchatka and Saõ Miguel Island</b></A><br /> <br /><i>Microbiology,
|
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249
|
+
Vol. , No. (2015) pp. - </i><br /><h3 class=&quot;a-plus-plus&quot;>Abstract</h3>\n
|
|
250
|
+
\ <p class=&quot;a-plus-plus&quot;>Detection
|
|
251
|
+
and analysis of the <em class=&quot;a-plus-plus&quot;>mcrA</em>
|
|
252
|
+
gene encoding methyl-coenzyme M reductase, the key enzyme of methanogenesis,
|
|
253
|
+
was used to assess occurrence and diversity of methanogenic archaea in terrestrial
|
|
254
|
+
hot springs of Kamchatka and Sa~o Miguel Island (the Azores). For this analysis,
|
|
255
|
+
phylogeny of methanogens was initially reconstructed based on available sequences
|
|
256
|
+
of the <em class=&quot;a-plus-plus&quot;>mcrA</em> gene,
|
|
257
|
+
which is a common functional and phylogenetic marker for this physiological
|
|
258
|
+
group of prokaryotes. Methanogens were revealed in most of the studied terrestrial
|
|
259
|
+
hot springs with temperatures from 51 to 89°C, although they constituted
|
|
260
|
+
an insignificant portion of the microbial population. The <em class=&quot;a-plus-plus&quot;>mcrA</em>
|
|
261
|
+
gene sequences revealed in the samples belonged to members of the genera <em
|
|
262
|
+
class=&quot;a-plus-plus&quot;>Methanothermobacter, Methanothermus</em>,
|
|
263
|
+
and <em class=&quot;a-plus-plus&quot;>Methanothrix</em>,
|
|
264
|
+
previously detected in hot springs, as well as to methanogens not found earlier
|
|
265
|
+
in these environments. The latter belonged to <em class=&quot;a-plus-plus&quot;>Methanomassiliicoccales,
|
|
266
|
+
Methanocellales</em>, and <em class=&quot;a-plus-plus&quot;>Methanomethylovorans</em>,
|
|
267
|
+
as well as to MCR-2a, the new deep phylogenetic cluster of uncultured methanogenic
|
|
268
|
+
archaea; its phylotypes were present in all springs where the <em class=&quot;a-plus-plus&quot;>mcrA</em>
|
|
269
|
+
gene was detected. Our results indicate high diversity of the thermophilic
|
|
270
|
+
methanogens inhabiting terrestrial hot springs and the presence among them
|
|
271
|
+
of new groups with yet unknown substrate specificity.</p></p>]]></content:encoded>\r\n</item>\n<item
|
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272
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+
rdf:about=\"http://link.springer.com/10.1134/S0026261715040086\">\n<title>Bacterial
|
|
273
|
+
photosensory proteins: Regulatory functions and optogenetic applications</title>\r\n<link>http://link.springer.com/10.1134/S0026261715040086</link>\r\n<description><h3
|
|
274
|
+
class=&quot;a-plus-plus&quot;>Abstract</h3>\n <p
|
|
275
|
+
class=&quot;a-plus-plus&quot;>Three classes of light-sensory regulatory
|
|
276
|
+
proteins, which have been identified in genomes of numerous phototrophic and
|
|
277
|
+
nonphotosynthetic bacteria, are discussed: the UVA/blue light sensitive BLUF
|
|
278
|
+
and LOV domain-containing proteins and red/far-red light-sensitive phytochromes.
|
|
279
|
+
Light perception by these chromoproteins is provided by the flavin or bilin
|
|
280
|
+
(in phytochromes) chromophores binding to their photosensory domains. Bacterial
|
|
281
|
+
photoreceptors also contain a variety of effector domains with enzymatic DNA-binding
|
|
282
|
+
and other functions, which compose modular light-switchable systems. In recent
|
|
283
|
+
years, progress was achieved in uncovering the photoactivation mechanisms
|
|
284
|
+
of such systems. Based on the chromophore phototransformation-induced changes
|
|
285
|
+
in the domain structures, these mechanisms cause the biochemical signal cascades
|
|
286
|
+
which can control the light-dependent physiological responses of the cells.
|
|
287
|
+
The new information obtained is important not only for understanding the fundamental
|
|
288
|
+
mechanisms of light perception and signal transduction by bacterial photosensory
|
|
289
|
+
proteins but also as a basis for designing photo-switchable enzymes and light-inducible
|
|
290
|
+
gene expression systems, which may be used in optogenetics, a new field in
|
|
291
|
+
cell biology and biotechnology. The presents review is focused on the structural
|
|
292
|
+
aspects of signal transduction in light-activated bacterial photoreceptors,
|
|
293
|
+
on their regulatory functions, and on some recent advances in using LOV and
|
|
294
|
+
BLUF photosensors in optogenetics for the regulation of biological processes.</p></description>\r\n<dc:identifier>http://link.springer.com/10.1134/S0026261715040086</dc:identifier>\r\n<dc:date>2015-07-01</dc:date>\n<dc:publisher>Springer-Verlag</dc:publisher>\n<prism:PublicationName>Microbiology</prism:PublicationName>\n<prism:publicationDate>2015-07-01</prism:publicationDate>\n<content:encoded><![CDATA[<p><a
|
|
295
|
+
href=\"http://link.springer.com/10.1134/S0026261715040086\"><b>Bacterial photosensory
|
|
296
|
+
proteins: Regulatory functions and optogenetic applications</b></A><br />
|
|
297
|
+
<br /><i>Microbiology, Vol. , No. (2015) pp. - </i><br /><h3 class=&quot;a-plus-plus&quot;>Abstract</h3>\n
|
|
298
|
+
\ <p class=&quot;a-plus-plus&quot;>Three classes
|
|
299
|
+
of light-sensory regulatory proteins, which have been identified in genomes
|
|
300
|
+
of numerous phototrophic and nonphotosynthetic bacteria, are discussed: the
|
|
301
|
+
UVA/blue light sensitive BLUF and LOV domain-containing proteins and red/far-red
|
|
302
|
+
light-sensitive phytochromes. Light perception by these chromoproteins is
|
|
303
|
+
provided by the flavin or bilin (in phytochromes) chromophores binding to
|
|
304
|
+
their photosensory domains. Bacterial photoreceptors also contain a variety
|
|
305
|
+
of effector domains with enzymatic DNA-binding and other functions, which
|
|
306
|
+
compose modular light-switchable systems. In recent years, progress was achieved
|
|
307
|
+
in uncovering the photoactivation mechanisms of such systems. Based on the
|
|
308
|
+
chromophore phototransformation-induced changes in the domain structures,
|
|
309
|
+
these mechanisms cause the biochemical signal cascades which can control the
|
|
310
|
+
light-dependent physiological responses of the cells. The new information
|
|
311
|
+
obtained is important not only for understanding the fundamental mechanisms
|
|
312
|
+
of light perception and signal transduction by bacterial photosensory proteins
|
|
313
|
+
but also as a basis for designing photo-switchable enzymes and light-inducible
|
|
314
|
+
gene expression systems, which may be used in optogenetics, a new field in
|
|
315
|
+
cell biology and biotechnology. The presents review is focused on the structural
|
|
316
|
+
aspects of signal transduction in light-activated bacterial photoreceptors,
|
|
317
|
+
on their regulatory functions, and on some recent advances in using LOV and
|
|
318
|
+
BLUF photosensors in optogenetics for the regulation of biological processes.</p></p>]]></content:encoded>\r\n</item>\n<item
|
|
319
|
+
rdf:about=\"http://link.springer.com/10.1134/S0026261715040153\">\n<title>The
|
|
320
|
+
production of exopolysaccharide by Pseudomonas putida GAP-P45 under various
|
|
321
|
+
abiotic stress conditions and its role in soil aggregation</title>\r\n<link>http://link.springer.com/10.1134/S0026261715040153</link>\r\n<description><h3
|
|
322
|
+
class=&quot;a-plus-plus&quot;>Abstract</h3>\n <p
|
|
323
|
+
class=&quot;a-plus-plus&quot;>Exopolysaccharides (EPS) production
|
|
324
|
+
is modified in response to environmental changes and is believed to protect
|
|
325
|
+
bacteria. In the present study <em class=&quot;a-plus-plus&quot;>Pseudomonas
|
|
326
|
+
putida</em> strain GAP-P45 identified by 16S rDNA sequence analysis
|
|
327
|
+
was exposed to stresses such as drought, temperature and salt to test the
|
|
328
|
+
ability to tolerate and produce EPS. Strain GAP-P45 could tolerate matric
|
|
329
|
+
stress up to −0.73 MPa, Temperature of 50°C and 1.4 M salt and
|
|
330
|
+
produced EPS, which increased with increase in stress levels. Among all stress
|
|
331
|
+
conditions, the production was high under drought stress. HPLC analysis revealed
|
|
332
|
+
that monosaccharide composition and ratio of sugars in EPS increased under
|
|
333
|
+
stress that might induce osmotic and thermal tolerance in GAP-P45. Rhamnose
|
|
334
|
+
was reported as major sugar under all stress conditions. Among the different
|
|
335
|
+
carbon sources tested, glycerol was found to be best for EPS production under
|
|
336
|
+
stressed as well as non-stressed conditions. Inoculation with GAP-P45 resulted
|
|
337
|
+
in better soil aggregation and aggregate stability under different stress
|
|
338
|
+
conditions. However inoculation effect was more under drought-stress. The
|
|
339
|
+
significance of these findings shows that abiotic stresses influence the EPS
|
|
340
|
+
composition and ratios of sugars, which influence the tolerance of the microorganisms
|
|
341
|
+
which can be employed for improvement in water holding capacity under unfavorable
|
|
342
|
+
environmental conditions.</p></description>\r\n<dc:identifier>http://link.springer.com/10.1134/S0026261715040153</dc:identifier>\r\n<dc:date>2015-07-01</dc:date>\n<dc:publisher>Springer-Verlag</dc:publisher>\n<prism:PublicationName>Microbiology</prism:PublicationName>\n<prism:publicationDate>2015-07-01</prism:publicationDate>\n<content:encoded><![CDATA[<p><a
|
|
343
|
+
href=\"http://link.springer.com/10.1134/S0026261715040153\"><b>The production
|
|
344
|
+
of exopolysaccharide by Pseudomonas putida GAP-P45 under various abiotic stress
|
|
345
|
+
conditions and its role in soil aggregation</b></A><br /> <br /><i>Microbiology,
|
|
346
|
+
Vol. , No. (2015) pp. - </i><br /><h3 class=&quot;a-plus-plus&quot;>Abstract</h3>\n
|
|
347
|
+
\ <p class=&quot;a-plus-plus&quot;>Exopolysaccharides
|
|
348
|
+
(EPS) production is modified in response to environmental changes and is believed
|
|
349
|
+
to protect bacteria. In the present study <em class=&quot;a-plus-plus&quot;>Pseudomonas
|
|
350
|
+
putida</em> strain GAP-P45 identified by 16S rDNA sequence analysis
|
|
351
|
+
was exposed to stresses such as drought, temperature and salt to test the
|
|
352
|
+
ability to tolerate and produce EPS. Strain GAP-P45 could tolerate matric
|
|
353
|
+
stress up to −0.73 MPa, Temperature of 50°C and 1.4 M salt and
|
|
354
|
+
produced EPS, which increased with increase in stress levels. Among all stress
|
|
355
|
+
conditions, the production was high under drought stress. HPLC analysis revealed
|
|
356
|
+
that monosaccharide composition and ratio of sugars in EPS increased under
|
|
357
|
+
stress that might induce osmotic and thermal tolerance in GAP-P45. Rhamnose
|
|
358
|
+
was reported as major sugar under all stress conditions. Among the different
|
|
359
|
+
carbon sources tested, glycerol was found to be best for EPS production under
|
|
360
|
+
stressed as well as non-stressed conditions. Inoculation with GAP-P45 resulted
|
|
361
|
+
in better soil aggregation and aggregate stability under different stress
|
|
362
|
+
conditions. However inoculation effect was more under drought-stress. The
|
|
363
|
+
significance of these findings shows that abiotic stresses influence the EPS
|
|
364
|
+
composition and ratios of sugars, which influence the tolerance of the microorganisms
|
|
365
|
+
which can be employed for improvement in water holding capacity under unfavorable
|
|
366
|
+
environmental conditions.</p></p>]]></content:encoded>\r\n</item>\n<item
|
|
367
|
+
rdf:about=\"http://link.springer.com/10.1134/S0026261715030157\">\n<title>Active
|
|
368
|
+
sulfate reduction in acidic sediments of gold mine tailings</title>\r\n<link>http://link.springer.com/10.1134/S0026261715030157</link>\r\n<description><br>\nArticle
|
|
369
|
+
URL: http://link.springer.com/10.1134/S0026261715030157<br>\nCitation:
|
|
370
|
+
\ (2015) <br>\nPublication Date: 2015-05-01<br>\nJournal: Microbiology</description>\r\n<dc:identifier>http://link.springer.com/10.1134/S0026261715030157</dc:identifier>\r\n<dc:date>2015-05-01</dc:date>\n<dc:publisher>Springer-Verlag</dc:publisher>\n<prism:PublicationName>Microbiology</prism:PublicationName>\n<prism:publicationDate>2015-05-01</prism:publicationDate>\n<content:encoded><![CDATA[<p><a
|
|
371
|
+
href=\"http://link.springer.com/10.1134/S0026261715030157\"><b>Active sulfate
|
|
372
|
+
reduction in acidic sediments of gold mine tailings</b></A><br /> <br /><i>Microbiology,
|
|
373
|
+
Vol. , No. (2015) pp. - </i><br />\nArticle URL: http://link.springer.com/10.1134/S0026261715030157\nCitation:
|
|
374
|
+
\ (2015) \nPublication Date: 2015-05-01\nJournal: Microbiology</p>]]></content:encoded>\r\n</item>\n<item
|
|
375
|
+
rdf:about=\"http://link.springer.com/10.1134/S0026261715030030\">\n<title>Activated
|
|
376
|
+
sludge bacteria transforming cyanopyridines and amides of pyridinecarboxylic
|
|
377
|
+
acids</title>\r\n<link>http://link.springer.com/10.1134/S0026261715030030</link>\r\n<description><h3
|
|
378
|
+
class=&quot;a-plus-plus&quot;>Abstract</h3>\n <p
|
|
379
|
+
class=&quot;a-plus-plus&quot;>Species diversity of bacteria from
|
|
380
|
+
the activated sludge of Perm biological waste treatment facilities capable
|
|
381
|
+
of transformation of cyanopyridines and amides of pyridinecarboxylic acids
|
|
382
|
+
was investigated. Enrichment cultures in mineral media with 3-cyanopyridine
|
|
383
|
+
as the sole carbon and nitrogen source were used to obtain 32 clones of gram-negative
|
|
384
|
+
heterotrophic bacteria exhibiting moderate growth on solid and liquid media
|
|
385
|
+
with 3- and 4-cyanopyridine. Sequencing of the 16S rRNA gene fragments revealed
|
|
386
|
+
that the clones with homology of at least 99% belonged to the genera <em
|
|
387
|
+
class=&quot;a-plus-plus&quot;>Acinetobacter</em>, <em
|
|
388
|
+
class=&quot;a-plus-plus&quot;>Alcaligenes</em>, <em class=&quot;a-plus-plus&quot;>Delftia</em>,
|
|
389
|
+
<em class=&quot;a-plus-plus&quot;>Ochrobactrum</em>, <em
|
|
390
|
+
class=&quot;a-plus-plus&quot;>Pseudomonas</em>, <em class=&quot;a-plus-plus&quot;>Stenotrophomonas</em>,
|
|
391
|
+
and <em class=&quot;a-plus-plus&quot;>Xanthobacter</em>.
|
|
392
|
+
PCR analysis showed that 13 out of 32 isolates contained the sequences (~1070
|
|
393
|
+
bp) homologous to the nitrilase genes reported previously in <em class=&quot;a-plus-plus&quot;>Alcaligenes
|
|
394
|
+
faecalis</em> JM3 (GenBank, D13419.1). Nine clones were capable of nitrile
|
|
395
|
+
and amide transformation when grown on minimal salt medium. <em class=&quot;a-plus-plus&quot;>Acinetobacter</em>
|
|
396
|
+
sp. 11h and <em class=&quot;a-plus-plus&quot;>Alcaligenes</em>
|
|
397
|
+
sp. osv transformed 3-cyanopyridine to nicotinamide, while most of the clones
|
|
398
|
+
possessed amidase activity (0.5 to 46.3 mmol/(g h) for acetamide and 0.1 to
|
|
399
|
+
5.6 mmol/(g h) for nicotinamide). Nicotinamide utilization by strain <em
|
|
400
|
+
class=&quot;a-plus-plus&quot;>A. faecalis</em> 2 was shown
|
|
401
|
+
to result in excretion of a secondary metabolite, which was identified as
|
|
402
|
+
dodecyl acrylate at 91% probability.</p></description>\r\n<dc:identifier>http://link.springer.com/10.1134/S0026261715030030</dc:identifier>\r\n<dc:date>2015-05-01</dc:date>\n<dc:publisher>Springer-Verlag</dc:publisher>\n<prism:PublicationName>Microbiology</prism:PublicationName>\n<prism:publicationDate>2015-05-01</prism:publicationDate>\n<content:encoded><![CDATA[<p><a
|
|
403
|
+
href=\"http://link.springer.com/10.1134/S0026261715030030\"><b>Activated sludge
|
|
404
|
+
bacteria transforming cyanopyridines and amides of pyridinecarboxylic acids</b></A><br
|
|
405
|
+
/> <br /><i>Microbiology, Vol. , No. (2015) pp. - </i><br /><h3 class=&quot;a-plus-plus&quot;>Abstract</h3>\n
|
|
406
|
+
\ <p class=&quot;a-plus-plus&quot;>Species
|
|
407
|
+
diversity of bacteria from the activated sludge of Perm biological waste treatment
|
|
408
|
+
facilities capable of transformation of cyanopyridines and amides of pyridinecarboxylic
|
|
409
|
+
acids was investigated. Enrichment cultures in mineral media with 3-cyanopyridine
|
|
410
|
+
as the sole carbon and nitrogen source were used to obtain 32 clones of gram-negative
|
|
411
|
+
heterotrophic bacteria exhibiting moderate growth on solid and liquid media
|
|
412
|
+
with 3- and 4-cyanopyridine. Sequencing of the 16S rRNA gene fragments revealed
|
|
413
|
+
that the clones with homology of at least 99% belonged to the genera <em
|
|
414
|
+
class=&quot;a-plus-plus&quot;>Acinetobacter</em>, <em
|
|
415
|
+
class=&quot;a-plus-plus&quot;>Alcaligenes</em>, <em class=&quot;a-plus-plus&quot;>Delftia</em>,
|
|
416
|
+
<em class=&quot;a-plus-plus&quot;>Ochrobactrum</em>, <em
|
|
417
|
+
class=&quot;a-plus-plus&quot;>Pseudomonas</em>, <em class=&quot;a-plus-plus&quot;>Stenotrophomonas</em>,
|
|
418
|
+
and <em class=&quot;a-plus-plus&quot;>Xanthobacter</em>.
|
|
419
|
+
PCR analysis showed that 13 out of 32 isolates contained the sequences (~1070
|
|
420
|
+
bp) homologous to the nitrilase genes reported previously in <em class=&quot;a-plus-plus&quot;>Alcaligenes
|
|
421
|
+
faecalis</em> JM3 (GenBank, D13419.1). Nine clones were capable of nitrile
|
|
422
|
+
and amide transformation when grown on minimal salt medium. <em class=&quot;a-plus-plus&quot;>Acinetobacter</em>
|
|
423
|
+
sp. 11h and <em class=&quot;a-plus-plus&quot;>Alcaligenes</em>
|
|
424
|
+
sp. osv transformed 3-cyanopyridine to nicotinamide, while most of the clones
|
|
425
|
+
possessed amidase activity (0.5 to 46.3 mmol/(g h) for acetamide and 0.1 to
|
|
426
|
+
5.6 mmol/(g h) for nicotinamide). Nicotinamide utilization by strain <em
|
|
427
|
+
class=&quot;a-plus-plus&quot;>A. faecalis</em> 2 was shown
|
|
428
|
+
to result in excretion of a secondary metabolite, which was identified as
|
|
429
|
+
dodecyl acrylate at 91% probability.</p></p>]]></content:encoded>\r\n</item>\n<item
|
|
430
|
+
rdf:about=\"http://link.springer.com/10.1134/S0026261715040074\">\n<title>1-aminocyclopropane-1-carboxylate
|
|
431
|
+
deaminase of the aerobic facultative methylotrophic actinomycete Amycolatopsis
|
|
432
|
+
methanolica 239</title>\r\n<link>http://link.springer.com/10.1134/S0026261715040074</link>\r\n<description><br>\nArticle
|
|
433
|
+
URL: http://link.springer.com/10.1134/S0026261715040074<br>\nCitation:
|
|
434
|
+
\ (2015) <br>\nPublication Date: 2015-07-01<br>\nJournal: Microbiology</description>\r\n<dc:identifier>http://link.springer.com/10.1134/S0026261715040074</dc:identifier>\r\n<dc:date>2015-07-01</dc:date>\n<dc:publisher>Springer-Verlag</dc:publisher>\n<prism:PublicationName>Microbiology</prism:PublicationName>\n<prism:publicationDate>2015-07-01</prism:publicationDate>\n<content:encoded><![CDATA[<p><a
|
|
435
|
+
href=\"http://link.springer.com/10.1134/S0026261715040074\"><b>1-aminocyclopropane-1-carboxylate
|
|
436
|
+
deaminase of the aerobic facultative methylotrophic actinomycete Amycolatopsis
|
|
437
|
+
methanolica 239</b></A><br /> <br /><i>Microbiology, Vol. , No. (2015) pp.
|
|
438
|
+
\ - </i><br />\nArticle URL: http://link.springer.com/10.1134/S0026261715040074\nCitation:
|
|
439
|
+
\ (2015) \nPublication Date: 2015-07-01\nJournal: Microbiology</p>]]></content:encoded>\r\n</item>\n<item
|
|
440
|
+
rdf:about=\"http://link.springer.com/10.1134/S0026261715040141\">\n<title>The
|
|
441
|
+
yeast Komagataella : A genetic genus in accordance with interspecies hybridization</title>\r\n<link>http://link.springer.com/10.1134/S0026261715040141</link>\r\n<description><h3
|
|
442
|
+
class=&quot;a-plus-plus&quot;>Abstract</h3>\n <p
|
|
443
|
+
class=&quot;a-plus-plus&quot;>Using induced complementary auxotrophic
|
|
444
|
+
mutants and selective growth of prototrophic hybrids on minimal medium, hybridization
|
|
445
|
+
of the type strain of <em class=&quot;a-plus-plus&quot;>Komagataella
|
|
446
|
+
kurtzmanii</em> VKPM Y-727 with the type strains of <em class=&quot;a-plus-plus&quot;>K.
|
|
447
|
+
pastoris</em> VKPM Y-3262, <em class=&quot;a-plus-plus&quot;>K.
|
|
448
|
+
phaffii</em> NRRL Y-7556, <em class=&quot;a-plus-plus&quot;>K.
|
|
449
|
+
populi</em> NRRL YB-455, <em class=&quot;a-plus-plus&quot;>K.
|
|
450
|
+
pseudopastoris</em> NRRL Y-27603, and <em class=&quot;a-plus-plus&quot;>K.
|
|
451
|
+
ulmi</em> NRRL YB-407 was demonstrated. The data obtained suggest that
|
|
452
|
+
the genus <em class=&quot;a-plus-plus&quot;>Komagataella</em>,
|
|
453
|
+
established previously by phylogenetic analysis, corresponds well to the concept
|
|
454
|
+
of genetic genus in ascomycetous fungi. According to this concept, a genetic
|
|
455
|
+
genus is a group of hybridized species having a common mating type system.
|
|
456
|
+
Application of the concept of genetic genus for different yeast genera is
|
|
457
|
+
discussed.</p></description>\r\n<dc:identifier>http://link.springer.com/10.1134/S0026261715040141</dc:identifier>\r\n<dc:date>2015-07-01</dc:date>\n<dc:publisher>Springer-Verlag</dc:publisher>\n<prism:PublicationName>Microbiology</prism:PublicationName>\n<prism:publicationDate>2015-07-01</prism:publicationDate>\n<content:encoded><![CDATA[<p><a
|
|
458
|
+
href=\"http://link.springer.com/10.1134/S0026261715040141\"><b>The yeast Komagataella
|
|
459
|
+
: A genetic genus in accordance with interspecies hybridization</b></A><br
|
|
460
|
+
/> <br /><i>Microbiology, Vol. , No. (2015) pp. - </i><br /><h3 class=&quot;a-plus-plus&quot;>Abstract</h3>\n
|
|
461
|
+
\ <p class=&quot;a-plus-plus&quot;>Using induced
|
|
462
|
+
complementary auxotrophic mutants and selective growth of prototrophic hybrids
|
|
463
|
+
on minimal medium, hybridization of the type strain of <em class=&quot;a-plus-plus&quot;>Komagataella
|
|
464
|
+
kurtzmanii</em> VKPM Y-727 with the type strains of <em class=&quot;a-plus-plus&quot;>K.
|
|
465
|
+
pastoris</em> VKPM Y-3262, <em class=&quot;a-plus-plus&quot;>K.
|
|
466
|
+
phaffii</em> NRRL Y-7556, <em class=&quot;a-plus-plus&quot;>K.
|
|
467
|
+
populi</em> NRRL YB-455, <em class=&quot;a-plus-plus&quot;>K.
|
|
468
|
+
pseudopastoris</em> NRRL Y-27603, and <em class=&quot;a-plus-plus&quot;>K.
|
|
469
|
+
ulmi</em> NRRL YB-407 was demonstrated. The data obtained suggest that
|
|
470
|
+
the genus <em class=&quot;a-plus-plus&quot;>Komagataella</em>,
|
|
471
|
+
established previously by phylogenetic analysis, corresponds well to the concept
|
|
472
|
+
of genetic genus in ascomycetous fungi. According to this concept, a genetic
|
|
473
|
+
genus is a group of hybridized species having a common mating type system.
|
|
474
|
+
Application of the concept of genetic genus for different yeast genera is
|
|
475
|
+
discussed.</p></p>]]></content:encoded>\r\n</item>\n<item rdf:about=\"http://link.springer.com/10.1134/S0026261715040104\">\n<title>Trophic
|
|
476
|
+
patterns of functioning and microbial profile of the evolutionally established
|
|
477
|
+
associated kefir grains culture</title>\r\n<link>http://link.springer.com/10.1134/S0026261715040104</link>\r\n<description><h3
|
|
478
|
+
class=&quot;a-plus-plus&quot;>Abstract</h3>\n <p
|
|
479
|
+
class=&quot;a-plus-plus&quot;>The associated culture of kefir grains
|
|
480
|
+
was analyzed by molecular methods for determination of the functional activity
|
|
481
|
+
of microbial isolates and molecular genetic techniques for their identification.
|
|
482
|
+
A combination of 16S rRNA analysis and denaturing gradient gel electrophoresis
|
|
483
|
+
was used to determine the microbial profile of kefir grains and to reveal
|
|
484
|
+
lactic acid bacteria of two physiological groups, differing in their ability
|
|
485
|
+
to use lactose for lactic acid fermentation. The role of inducible β-galactosidase
|
|
486
|
+
of lactic acid bacteria for the functional stability of the microbial community
|
|
487
|
+
was shown in the study of the functional activity and microbial profile of
|
|
488
|
+
the kefir grains after long-time cultivation (over 4 years) on lactose-free
|
|
489
|
+
milk. The results obtained improve our understanding of the possible trophic
|
|
490
|
+
interactions in such microbial communities and may be used to develop the
|
|
491
|
+
algorithm for experimental production of a stably associated culture of kefir
|
|
492
|
+
grains.</p></description>\r\n<dc:identifier>http://link.springer.com/10.1134/S0026261715040104</dc:identifier>\r\n<dc:date>2015-07-01</dc:date>\n<dc:publisher>Springer-Verlag</dc:publisher>\n<prism:PublicationName>Microbiology</prism:PublicationName>\n<prism:publicationDate>2015-07-01</prism:publicationDate>\n<content:encoded><![CDATA[<p><a
|
|
493
|
+
href=\"http://link.springer.com/10.1134/S0026261715040104\"><b>Trophic patterns
|
|
494
|
+
of functioning and microbial profile of the evolutionally established associated
|
|
495
|
+
kefir grains culture</b></A><br /> <br /><i>Microbiology, Vol. , No. (2015)
|
|
496
|
+
pp. - </i><br /><h3 class=&quot;a-plus-plus&quot;>Abstract</h3>\n
|
|
497
|
+
\ <p class=&quot;a-plus-plus&quot;>The associated
|
|
498
|
+
culture of kefir grains was analyzed by molecular methods for determination
|
|
499
|
+
of the functional activity of microbial isolates and molecular genetic techniques
|
|
500
|
+
for their identification. A combination of 16S rRNA analysis and denaturing
|
|
501
|
+
gradient gel electrophoresis was used to determine the microbial profile of
|
|
502
|
+
kefir grains and to reveal lactic acid bacteria of two physiological groups,
|
|
503
|
+
differing in their ability to use lactose for lactic acid fermentation. The
|
|
504
|
+
role of inducible β-galactosidase of lactic acid bacteria for the functional
|
|
505
|
+
stability of the microbial community was shown in the study of the functional
|
|
506
|
+
activity and microbial profile of the kefir grains after long-time cultivation
|
|
507
|
+
(over 4 years) on lactose-free milk. The results obtained improve our understanding
|
|
508
|
+
of the possible trophic interactions in such microbial communities and may
|
|
509
|
+
be used to develop the algorithm for experimental production of a stably associated
|
|
510
|
+
culture of kefir grains.</p></p>]]></content:encoded>\r\n</item>\n<item
|
|
511
|
+
rdf:about=\"http://link.springer.com/10.1134/S0026261715040062\">\n<title>Disruption
|
|
512
|
+
of bacterial biofilms using recombinant dispersin B</title>\r\n<link>http://link.springer.com/10.1134/S0026261715040062</link>\r\n<description><h3
|
|
513
|
+
class=&quot;a-plus-plus&quot;>Abstract</h3>\n <p
|
|
514
|
+
class=&quot;a-plus-plus&quot;>A synthetic gene encoding dispersin
|
|
515
|
+
B of <em class=&quot;a-plus-plus&quot;>Aggregatibacter actinomycetemcomitans</em>
|
|
516
|
+
was cloned and expressed in <em class=&quot;a-plus-plus&quot;>Escherichia
|
|
517
|
+
coli</em> cells. Procedure for purification of recombinant dispersin
|
|
518
|
+
B was developed, and its in vitro activity was determined. The enzyme was
|
|
519
|
+
used in experiments on disruption of the biofilms formed by various microorganisms.
|
|
520
|
+
It exhibited high activity against <em class=&quot;a-plus-plus&quot;>Staphylococcus
|
|
521
|
+
epidermidis</em> biofilms. The biofilms formed by <em class=&quot;a-plus-plus&quot;>Burkholderia
|
|
522
|
+
cenocepacia</em> and <em class=&quot;a-plus-plus&quot;>Achromobacter
|
|
523
|
+
xylosoxidans</em> were more resistant to the recombinant enzyme.</p></description>\r\n<dc:identifier>http://link.springer.com/10.1134/S0026261715040062</dc:identifier>\r\n<dc:date>2015-07-01</dc:date>\n<dc:publisher>Springer-Verlag</dc:publisher>\n<prism:PublicationName>Microbiology</prism:PublicationName>\n<prism:publicationDate>2015-07-01</prism:publicationDate>\n<content:encoded><![CDATA[<p><a
|
|
524
|
+
href=\"http://link.springer.com/10.1134/S0026261715040062\"><b>Disruption
|
|
525
|
+
of bacterial biofilms using recombinant dispersin B</b></A><br /> <br /><i>Microbiology,
|
|
526
|
+
Vol. , No. (2015) pp. - </i><br /><h3 class=&quot;a-plus-plus&quot;>Abstract</h3>\n
|
|
527
|
+
\ <p class=&quot;a-plus-plus&quot;>A synthetic
|
|
528
|
+
gene encoding dispersin B of <em class=&quot;a-plus-plus&quot;>Aggregatibacter
|
|
529
|
+
actinomycetemcomitans</em> was cloned and expressed in <em class=&quot;a-plus-plus&quot;>Escherichia
|
|
530
|
+
coli</em> cells. Procedure for purification of recombinant dispersin
|
|
531
|
+
B was developed, and its in vitro activity was determined. The enzyme was
|
|
532
|
+
used in experiments on disruption of the biofilms formed by various microorganisms.
|
|
533
|
+
It exhibited high activity against <em class=&quot;a-plus-plus&quot;>Staphylococcus
|
|
534
|
+
epidermidis</em> biofilms. The biofilms formed by <em class=&quot;a-plus-plus&quot;>Burkholderia
|
|
535
|
+
cenocepacia</em> and <em class=&quot;a-plus-plus&quot;>Achromobacter
|
|
536
|
+
xylosoxidans</em> were more resistant to the recombinant enzyme.</p></p>]]></content:encoded>\r\n</item>\n<item
|
|
537
|
+
rdf:about=\"http://link.springer.com/10.1134/S0026261715040025\">\n<title>
|
|
538
|
+
Methylopila turkiensis sp. nov., a new aerobic facultatively methylotrophic
|
|
539
|
+
phytosymbiont</title>\r\n<link>http://link.springer.com/10.1134/S0026261715040025</link>\r\n<description><h3
|
|
540
|
+
class=&quot;a-plus-plus&quot;>Abstract</h3>\n <p
|
|
541
|
+
class=&quot;a-plus-plus&quot;>A new facultative methylotroph, strain
|
|
542
|
+
Side1<sup class=&quot;a-plus-plus&quot;>T</sup>, was isolated
|
|
543
|
+
from the phyllosphere of <em class=&quot;a-plus-plus&quot;>Bougainvillea</em>
|
|
544
|
+
sp. L. The isolate is represented by rod-shaped, aerobic gram-negative asporogenous
|
|
545
|
+
bacteria which divide by binary fission. Methanol and mono- and trimethylamine
|
|
546
|
+
were utilized, as well as a limited spectrum of polycarbon substrates, while
|
|
547
|
+
methane and dichloromethane were not used. Growth occurred at pH 6.0–9.0
|
|
548
|
+
with the optimum at pH 7.0 within the temperature range from 20 to 40°C
|
|
549
|
+
(optimum at 28–30°C) and 0–2.5% NaCl in the medium. The predominant
|
|
550
|
+
fatty acids were <em class=&quot;a-plus-plus&quot;>cis</em>-11-octadecenoic
|
|
551
|
+
(C18:1ω7c), 11-methyl-octadecenoic (C18:ω7c11Me), and stearic (C18:0)
|
|
552
|
+
acids. Phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol,
|
|
553
|
+
and diphosphatidylglycerol were the dominant phospholipids. Q<sub class=&quot;a-plus-plus&quot;>10</sub>
|
|
554
|
+
was the dominant ubiquinone. The isolate oxidized methanol and methylamine
|
|
555
|
+
by the appropriate dehydrogenases. The isocitrate lyase-negative variant of
|
|
556
|
+
the serine pathway was used. Ammonium assimilation involved glutamate dehydrogenase
|
|
557
|
+
and the glutamate cycle (glutamate synthase and glutamine synthetase). The
|
|
558
|
+
strain synthesized indole and siderophores; it solubilized insoluble phosphates.
|
|
559
|
+
The DNA G+C content (<em class=&quot;a-plus-plus&quot;>T</em>\n
|
|
560
|
+
\ <sub class=&quot;a-plus-plus&quot;>m</sub>)
|
|
561
|
+
was 65.4 mol %. While the nucleotide sequence of the 16S rRNA gene of strain
|
|
562
|
+
Side1 exhibited high similarity to those of <em class=&quot;a-plus-plus&quot;>Methylopila</em>
|
|
563
|
+
species (<em class=&quot;a-plus-plus&quot;>M. musalis</em>
|
|
564
|
+
MUSA<sup class=&quot;a-plus-plus&quot;>T</sup> and <em
|
|
565
|
+
class=&quot;a-plus-plus&quot;>M. capsulata</em> IM1<sup
|
|
566
|
+
class=&quot;a-plus-plus&quot;>T</sup>), DNA-DNA homology
|
|
567
|
+
with these cultures was 32–37%. The results obtained supported classification
|
|
568
|
+
of strain Side1<sup class=&quot;a-plus-plus&quot;>T</sup>
|
|
569
|
+
as a new species <em class=&quot;a-plus-plus&quot;>Methylopila
|
|
570
|
+
turkiensis</em> sp. nov. (VKM B-2748<sup class=&quot;a-plus-plus&quot;>T</sup>=
|
|
571
|
+
DSM 27566<sup class=&quot;a-plus-plus&quot;>T</sup>).</p></description>\r\n<dc:identifier>http://link.springer.com/10.1134/S0026261715040025</dc:identifier>\r\n<dc:date>2015-07-01</dc:date>\n<dc:publisher>Springer-Verlag</dc:publisher>\n<prism:PublicationName>Microbiology</prism:PublicationName>\n<prism:publicationDate>2015-07-01</prism:publicationDate>\n<content:encoded><![CDATA[<p><a
|
|
572
|
+
href=\"http://link.springer.com/10.1134/S0026261715040025\"><b> Methylopila
|
|
573
|
+
turkiensis sp. nov., a new aerobic facultatively methylotrophic phytosymbiont</b></A><br
|
|
574
|
+
/> <br /><i>Microbiology, Vol. , No. (2015) pp. - </i><br /><h3 class=&quot;a-plus-plus&quot;>Abstract</h3>\n
|
|
575
|
+
\ <p class=&quot;a-plus-plus&quot;>A new facultative
|
|
576
|
+
methylotroph, strain Side1<sup class=&quot;a-plus-plus&quot;>T</sup>,
|
|
577
|
+
was isolated from the phyllosphere of <em class=&quot;a-plus-plus&quot;>Bougainvillea</em>
|
|
578
|
+
sp. L. The isolate is represented by rod-shaped, aerobic gram-negative asporogenous
|
|
579
|
+
bacteria which divide by binary fission. Methanol and mono- and trimethylamine
|
|
580
|
+
were utilized, as well as a limited spectrum of polycarbon substrates, while
|
|
581
|
+
methane and dichloromethane were not used. Growth occurred at pH 6.0–9.0
|
|
582
|
+
with the optimum at pH 7.0 within the temperature range from 20 to 40°C
|
|
583
|
+
(optimum at 28–30°C) and 0–2.5% NaCl in the medium. The predominant
|
|
584
|
+
fatty acids were <em class=&quot;a-plus-plus&quot;>cis</em>-11-octadecenoic
|
|
585
|
+
(C18:1ω7c), 11-methyl-octadecenoic (C18:ω7c11Me), and stearic (C18:0)
|
|
586
|
+
acids. Phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol,
|
|
587
|
+
and diphosphatidylglycerol were the dominant phospholipids. Q<sub class=&quot;a-plus-plus&quot;>10</sub>
|
|
588
|
+
was the dominant ubiquinone. The isolate oxidized methanol and methylamine
|
|
589
|
+
by the appropriate dehydrogenases. The isocitrate lyase-negative variant of
|
|
590
|
+
the serine pathway was used. Ammonium assimilation involved glutamate dehydrogenase
|
|
591
|
+
and the glutamate cycle (glutamate synthase and glutamine synthetase). The
|
|
592
|
+
strain synthesized indole and siderophores; it solubilized insoluble phosphates.
|
|
593
|
+
The DNA G+C content (<em class=&quot;a-plus-plus&quot;>T</em>\n
|
|
594
|
+
\ <sub class=&quot;a-plus-plus&quot;>m</sub>)
|
|
595
|
+
was 65.4 mol %. While the nucleotide sequence of the 16S rRNA gene of strain
|
|
596
|
+
Side1 exhibited high similarity to those of <em class=&quot;a-plus-plus&quot;>Methylopila</em>
|
|
597
|
+
species (<em class=&quot;a-plus-plus&quot;>M. musalis</em>
|
|
598
|
+
MUSA<sup class=&quot;a-plus-plus&quot;>T</sup> and <em
|
|
599
|
+
class=&quot;a-plus-plus&quot;>M. capsulata</em> IM1<sup
|
|
600
|
+
class=&quot;a-plus-plus&quot;>T</sup>), DNA-DNA homology
|
|
601
|
+
with these cultures was 32–37%. The results obtained supported classification
|
|
602
|
+
of strain Side1<sup class=&quot;a-plus-plus&quot;>T</sup>
|
|
603
|
+
as a new species <em class=&quot;a-plus-plus&quot;>Methylopila
|
|
604
|
+
turkiensis</em> sp. nov. (VKM B-2748<sup class=&quot;a-plus-plus&quot;>T</sup>=
|
|
605
|
+
DSM 27566<sup class=&quot;a-plus-plus&quot;>T</sup>).</p></p>]]></content:encoded>\r\n</item>\n<item
|
|
606
|
+
rdf:about=\"http://link.springer.com/10.1134/S0026261715040037\">\n<title>Statistical
|
|
607
|
+
medium optimization for the production of collagenolytic protease by Pseudomonas
|
|
608
|
+
sp. SUK using response surface methodology</title>\r\n<link>http://link.springer.com/10.1134/S0026261715040037</link>\r\n<description><h3
|
|
609
|
+
class=&quot;a-plus-plus&quot;>Abstract</h3>\n <p
|
|
610
|
+
class=&quot;a-plus-plus&quot;>\n <em
|
|
611
|
+
class=&quot;a-plus-plus&quot;>Pseudomonas</em> sp. SUK producing
|
|
612
|
+
an extracellular collagenolytic protease was isolated from soil samples from
|
|
613
|
+
meat and poultry industrial area based in Kolhapur, India. Response surface
|
|
614
|
+
methodology was employed for the optimization of different nutritional parameters
|
|
615
|
+
influencing production of collagenolytic protease by newly isolated <em
|
|
616
|
+
class=&quot;a-plus-plus&quot;>Pseudomonas</em> sp. SUK in
|
|
617
|
+
submerged fermentation. Initial screening of production parameters was performed
|
|
618
|
+
using Plackett-Burman design and the variables with statistically significant
|
|
619
|
+
effects on collagenolytic protease production were identified as gelatin,
|
|
620
|
+
peptone, and K<sub class=&quot;a-plus-plus&quot;>2</sub>HPO<sub
|
|
621
|
+
class=&quot;a-plus-plus&quot;>4</sub>. Further, optimization
|
|
622
|
+
by response surface methodology (RSM) using Central Composite Design showed
|
|
623
|
+
optimum production of collagenolytic protease with 12.05 g L<sup class=&quot;a-plus-plus&quot;>−1</sup>
|
|
624
|
+
of gelatin, 12.26 g L<sup class=&quot;a-plus-plus&quot;>−1</sup>
|
|
625
|
+
of peptone and 1.29 g L<sup class=&quot;a-plus-plus&quot;>−1</sup>
|
|
626
|
+
of K<sub class=&quot;a-plus-plus&quot;>2</sub>HPO<sub
|
|
627
|
+
class=&quot;a-plus-plus&quot;>4</sub>. Collagenolytic protease
|
|
628
|
+
production obtained experimentally has very close agreement with the model
|
|
629
|
+
prediction value and the model was proven to be adequate. The statistical
|
|
630
|
+
optimization by response surface methodology upsurges collagenolytic protease
|
|
631
|
+
yield by 2.9 fold, hence the experimental design is effective towards process
|
|
632
|
+
optimization. Moreover, ammonium sulphate precipitated, partially purified
|
|
633
|
+
enzyme has shown to cleave collagen from bovine achilles tendon, which was
|
|
634
|
+
observed by phase contrast microscopy, and SDS-PAGE. Hence, extracellular
|
|
635
|
+
collagenolytic protease of <em class=&quot;a-plus-plus&quot;>Pseudomonas</em>
|
|
636
|
+
sp. SUK could have considerable potential for industrial as well as medical
|
|
637
|
+
applications.</p></description>\r\n<dc:identifier>http://link.springer.com/10.1134/S0026261715040037</dc:identifier>\r\n<dc:date>2015-07-01</dc:date>\n<dc:publisher>Springer-Verlag</dc:publisher>\n<prism:PublicationName>Microbiology</prism:PublicationName>\n<prism:publicationDate>2015-07-01</prism:publicationDate>\n<content:encoded><![CDATA[<p><a
|
|
638
|
+
href=\"http://link.springer.com/10.1134/S0026261715040037\"><b>Statistical
|
|
639
|
+
medium optimization for the production of collagenolytic protease by Pseudomonas
|
|
640
|
+
sp. SUK using response surface methodology</b></A><br /> <br /><i>Microbiology,
|
|
641
|
+
Vol. , No. (2015) pp. - </i><br /><h3 class=&quot;a-plus-plus&quot;>Abstract</h3>\n
|
|
642
|
+
\ <p class=&quot;a-plus-plus&quot;>\n <em
|
|
643
|
+
class=&quot;a-plus-plus&quot;>Pseudomonas</em> sp. SUK producing
|
|
644
|
+
an extracellular collagenolytic protease was isolated from soil samples from
|
|
645
|
+
meat and poultry industrial area based in Kolhapur, India. Response surface
|
|
646
|
+
methodology was employed for the optimization of different nutritional parameters
|
|
647
|
+
influencing production of collagenolytic protease by newly isolated <em
|
|
648
|
+
class=&quot;a-plus-plus&quot;>Pseudomonas</em> sp. SUK in
|
|
649
|
+
submerged fermentation. Initial screening of production parameters was performed
|
|
650
|
+
using Plackett-Burman design and the variables with statistically significant
|
|
651
|
+
effects on collagenolytic protease production were identified as gelatin,
|
|
652
|
+
peptone, and K<sub class=&quot;a-plus-plus&quot;>2</sub>HPO<sub
|
|
653
|
+
class=&quot;a-plus-plus&quot;>4</sub>. Further, optimization
|
|
654
|
+
by response surface methodology (RSM) using Central Composite Design showed
|
|
655
|
+
optimum production of collagenolytic protease with 12.05 g L<sup class=&quot;a-plus-plus&quot;>−1</sup>
|
|
656
|
+
of gelatin, 12.26 g L<sup class=&quot;a-plus-plus&quot;>−1</sup>
|
|
657
|
+
of peptone and 1.29 g L<sup class=&quot;a-plus-plus&quot;>−1</sup>
|
|
658
|
+
of K<sub class=&quot;a-plus-plus&quot;>2</sub>HPO<sub
|
|
659
|
+
class=&quot;a-plus-plus&quot;>4</sub>. Collagenolytic protease
|
|
660
|
+
production obtained experimentally has very close agreement with the model
|
|
661
|
+
prediction value and the model was proven to be adequate. The statistical
|
|
662
|
+
optimization by response surface methodology upsurges collagenolytic protease
|
|
663
|
+
yield by 2.9 fold, hence the experimental design is effective towards process
|
|
664
|
+
optimization. Moreover, ammonium sulphate precipitated, partially purified
|
|
665
|
+
enzyme has shown to cleave collagen from bovine achilles tendon, which was
|
|
666
|
+
observed by phase contrast microscopy, and SDS-PAGE. Hence, extracellular
|
|
667
|
+
collagenolytic protease of <em class=&quot;a-plus-plus&quot;>Pseudomonas</em>
|
|
668
|
+
sp. SUK could have considerable potential for industrial as well as medical
|
|
669
|
+
applications.</p></p>]]></content:encoded>\r\n</item>\n<item rdf:about=\"http://link.springer.com/10.1134/S0026261715040189\">\n<title>Structural
|
|
670
|
+
characterization of the extracellular peptide metabolites of Luteococcus japonicus
|
|
671
|
+
subsp. casei and their protective effect on probiotic bacteria</title>\r\n<link>http://link.springer.com/10.1134/S0026261715040189</link>\r\n<description><h3
|
|
672
|
+
class=&quot;a-plus-plus&quot;>Abstract</h3>\n <p
|
|
673
|
+
class=&quot;a-plus-plus&quot;>Protective effect of <em class=&quot;a-plus-plus&quot;>Luteococcus
|
|
674
|
+
japonicus</em> subsp. <em class=&quot;a-plus-plus&quot;>casei</em>
|
|
675
|
+
exometabolites on the cells of transient probiotic <em class=&quot;a-plus-plus&quot;>Propionibacterium</em>
|
|
676
|
+
strains, <em class=&quot;a-plus-plus&quot;>Enterococcus faecium</em>,
|
|
677
|
+
and the yeasts <em class=&quot;a-plus-plus&quot;>Saccharomyces
|
|
678
|
+
cerevisiae</em> Boulardii under exposure to bile salts (BS) and acid
|
|
679
|
+
stress was studied. The extracellular peptide reactivating factor (RF) and
|
|
680
|
+
the peptide component of the culture liquid (CL) after RF removal possessed
|
|
681
|
+
a protective effect. Protective (preventive) and reactivating (after stress
|
|
682
|
+
impact) application of RF resulted in 1.5- to 2.0-fold increased survival
|
|
683
|
+
of the human transient probiotics <em class=&quot;a-plus-plus&quot;>P.
|
|
684
|
+
freudenreichii, P. acidipropionici</em> and <em class=&quot;a-plus-plus&quot;>E.
|
|
685
|
+
faecium</em> subjected to BS treatment of acid stress. The CL peptide
|
|
686
|
+
fraction had a stronger protective effect. Its application for preincubation
|
|
687
|
+
of <em class=&quot;a-plus-plus&quot;>P. acidipropionici</em>
|
|
688
|
+
cells resulted in 14-fold (BS treatment) and 8-fold (acid stress) increased
|
|
689
|
+
survival compared to the control. Yeasts exhibited very high resistance to
|
|
690
|
+
the stress factors used. Two active glycopeptide fractions with molecular
|
|
691
|
+
masses of 1.8 and 2.4 kDa were found in RF. Analysis of their amino acid composition
|
|
692
|
+
revealed the residues of glycine, leucine, proline, arginine, and aspartate/asparagine,
|
|
693
|
+
while no residues of aromatic and sulfur-containing amino acids, or the disulfide
|
|
694
|
+
bridge-type posttranslational modifications, were found. The possible mechanisms
|
|
695
|
+
of reactivating and the protective effect of RF are discussed.</p></description>\r\n<dc:identifier>http://link.springer.com/10.1134/S0026261715040189</dc:identifier>\r\n<dc:date>2015-07-01</dc:date>\n<dc:publisher>Springer-Verlag</dc:publisher>\n<prism:PublicationName>Microbiology</prism:PublicationName>\n<prism:publicationDate>2015-07-01</prism:publicationDate>\n<content:encoded><![CDATA[<p><a
|
|
696
|
+
href=\"http://link.springer.com/10.1134/S0026261715040189\"><b>Structural
|
|
697
|
+
characterization of the extracellular peptide metabolites of Luteococcus japonicus
|
|
698
|
+
subsp. casei and their protective effect on probiotic bacteria</b></A><br
|
|
699
|
+
/> <br /><i>Microbiology, Vol. , No. (2015) pp. - </i><br /><h3 class=&quot;a-plus-plus&quot;>Abstract</h3>\n
|
|
700
|
+
\ <p class=&quot;a-plus-plus&quot;>Protective
|
|
701
|
+
effect of <em class=&quot;a-plus-plus&quot;>Luteococcus japonicus</em>
|
|
702
|
+
subsp. <em class=&quot;a-plus-plus&quot;>casei</em> exometabolites
|
|
703
|
+
on the cells of transient probiotic <em class=&quot;a-plus-plus&quot;>Propionibacterium</em>
|
|
704
|
+
strains, <em class=&quot;a-plus-plus&quot;>Enterococcus faecium</em>,
|
|
705
|
+
and the yeasts <em class=&quot;a-plus-plus&quot;>Saccharomyces
|
|
706
|
+
cerevisiae</em> Boulardii under exposure to bile salts (BS) and acid
|
|
707
|
+
stress was studied. The extracellular peptide reactivating factor (RF) and
|
|
708
|
+
the peptide component of the culture liquid (CL) after RF removal possessed
|
|
709
|
+
a protective effect. Protective (preventive) and reactivating (after stress
|
|
710
|
+
impact) application of RF resulted in 1.5- to 2.0-fold increased survival
|
|
711
|
+
of the human transient probiotics <em class=&quot;a-plus-plus&quot;>P.
|
|
712
|
+
freudenreichii, P. acidipropionici</em> and <em class=&quot;a-plus-plus&quot;>E.
|
|
713
|
+
faecium</em> subjected to BS treatment of acid stress. The CL peptide
|
|
714
|
+
fraction had a stronger protective effect. Its application for preincubation
|
|
715
|
+
of <em class=&quot;a-plus-plus&quot;>P. acidipropionici</em>
|
|
716
|
+
cells resulted in 14-fold (BS treatment) and 8-fold (acid stress) increased
|
|
717
|
+
survival compared to the control. Yeasts exhibited very high resistance to
|
|
718
|
+
the stress factors used. Two active glycopeptide fractions with molecular
|
|
719
|
+
masses of 1.8 and 2.4 kDa were found in RF. Analysis of their amino acid composition
|
|
720
|
+
revealed the residues of glycine, leucine, proline, arginine, and aspartate/asparagine,
|
|
721
|
+
while no residues of aromatic and sulfur-containing amino acids, or the disulfide
|
|
722
|
+
bridge-type posttranslational modifications, were found. The possible mechanisms
|
|
723
|
+
of reactivating and the protective effect of RF are discussed.</p></p>]]></content:encoded>\r\n</item>\n<item
|
|
724
|
+
rdf:about=\"http://link.springer.com/10.1134/S0026261715030194\">\n<title>Formation
|
|
725
|
+
of 55-kDa fragments under impaired coordination bonds and hydrophobic interactions
|
|
726
|
+
in peripheral light-harvesting complexes isolated from photosynthetic purple
|
|
727
|
+
bacteria</title>\r\n<link>http://link.springer.com/10.1134/S0026261715030194</link>\r\n<description><h3
|
|
728
|
+
class=&quot;a-plus-plus&quot;>Abstract</h3>\n <p
|
|
729
|
+
class=&quot;a-plus-plus&quot;>Size exclusion chromatography was
|
|
730
|
+
used to assess the relative size of intact and diphenylamine-treated (DPA,
|
|
731
|
+
with suppressed carotenoid synthesis) peripheral light-harvesting complexes
|
|
732
|
+
(LH2 complexes) of the sulfur bacterium <em class=&quot;a-plus-plus&quot;>Allochromatium
|
|
733
|
+
minutissimum</em>. Both LH2 complexes were nonamers and had the same
|
|
734
|
+
elution volume V<sub class=&quot;a-plus-plus&quot;>e</sub>,
|
|
735
|
+
coinciding with that for the LH2 complex of <em class=&quot;a-plus-plus&quot;>Rhodoblastus
|
|
736
|
+
acidophilus</em> (strain 10050). Their molecular weight was 150 kDa.
|
|
737
|
+
Both pheophytinization of bacteriochlorophyll (BChl) at low pH and treatment
|
|
738
|
+
with the detergent LDAO, which affects the hydrophobic interactions between
|
|
739
|
+
the neighboring protomers, result in the fragmentation of the ring of the
|
|
740
|
+
isolated LH2 complexes and formation of 55-kDa fragments with molecular weights
|
|
741
|
+
corresponding to one-third of the initial value. Fragmentation caused by both
|
|
742
|
+
pheophytinization and detergent treatment was much more rapid in DPA LH2 complexes
|
|
743
|
+
than in the intact ones. The 55-kDa fragments formed at low pH values contained
|
|
744
|
+
monomeric bacteriopheophytin, while the fragments of a similar molecular weight
|
|
745
|
+
formed at pH 8.0 in the presence of the detergent contained monomeric BChl.
|
|
746
|
+
The observed fragmentation was hypothesized to reflect the inherent C<sub
|
|
747
|
+
class=&quot;a-plus-plus&quot;>3</sub> symmetry of the LH2
|
|
748
|
+
complexes, with the preliminarily assembled trimers used as building blocks.</p></description>\r\n<dc:identifier>http://link.springer.com/10.1134/S0026261715030194</dc:identifier>\r\n<dc:date>2015-05-01</dc:date>\n<dc:publisher>Springer-Verlag</dc:publisher>\n<prism:PublicationName>Microbiology</prism:PublicationName>\n<prism:publicationDate>2015-05-01</prism:publicationDate>\n<content:encoded><![CDATA[<p><a
|
|
749
|
+
href=\"http://link.springer.com/10.1134/S0026261715030194\"><b>Formation of
|
|
750
|
+
55-kDa fragments under impaired coordination bonds and hydrophobic interactions
|
|
751
|
+
in peripheral light-harvesting complexes isolated from photosynthetic purple
|
|
752
|
+
bacteria</b></A><br /> <br /><i>Microbiology, Vol. , No. (2015) pp. - </i><br
|
|
753
|
+
/><h3 class=&quot;a-plus-plus&quot;>Abstract</h3>\n <p
|
|
754
|
+
class=&quot;a-plus-plus&quot;>Size exclusion chromatography was
|
|
755
|
+
used to assess the relative size of intact and diphenylamine-treated (DPA,
|
|
756
|
+
with suppressed carotenoid synthesis) peripheral light-harvesting complexes
|
|
757
|
+
(LH2 complexes) of the sulfur bacterium <em class=&quot;a-plus-plus&quot;>Allochromatium
|
|
758
|
+
minutissimum</em>. Both LH2 complexes were nonamers and had the same
|
|
759
|
+
elution volume V<sub class=&quot;a-plus-plus&quot;>e</sub>,
|
|
760
|
+
coinciding with that for the LH2 complex of <em class=&quot;a-plus-plus&quot;>Rhodoblastus
|
|
761
|
+
acidophilus</em> (strain 10050). Their molecular weight was 150 kDa.
|
|
762
|
+
Both pheophytinization of bacteriochlorophyll (BChl) at low pH and treatment
|
|
763
|
+
with the detergent LDAO, which affects the hydrophobic interactions between
|
|
764
|
+
the neighboring protomers, result in the fragmentation of the ring of the
|
|
765
|
+
isolated LH2 complexes and formation of 55-kDa fragments with molecular weights
|
|
766
|
+
corresponding to one-third of the initial value. Fragmentation caused by both
|
|
767
|
+
pheophytinization and detergent treatment was much more rapid in DPA LH2 complexes
|
|
768
|
+
than in the intact ones. The 55-kDa fragments formed at low pH values contained
|
|
769
|
+
monomeric bacteriopheophytin, while the fragments of a similar molecular weight
|
|
770
|
+
formed at pH 8.0 in the presence of the detergent contained monomeric BChl.
|
|
771
|
+
The observed fragmentation was hypothesized to reflect the inherent C<sub
|
|
772
|
+
class=&quot;a-plus-plus&quot;>3</sub> symmetry of the LH2
|
|
773
|
+
complexes, with the preliminarily assembled trimers used as building blocks.</p></p>]]></content:encoded>\r\n</item>\n<item
|
|
774
|
+
rdf:about=\"http://link.springer.com/10.1134/S0026261715040177\">\n<title>Preparations
|
|
775
|
+
of Bacillus pumilus secreted RNase: One enzyme or two'</title>\r\n<link>http://link.springer.com/10.1134/S0026261715040177</link>\r\n<description><h3
|
|
776
|
+
class=&quot;a-plus-plus&quot;>Abstract</h3>\n <p
|
|
777
|
+
class=&quot;a-plus-plus&quot;>Immunochemical analysis of the following
|
|
778
|
+
purified preparations of <em class=&quot;a-plus-plus&quot;>Bacillus
|
|
779
|
+
pumilus</em> RNase (binase) was carried out: industrially manufactured
|
|
780
|
+
enzyme (Institute of Organic Synthesis, Riga, Latvia) and the enzymes isolated
|
|
781
|
+
from the culture liquid of the native <em class=&quot;a-plus-plus&quot;>B.
|
|
782
|
+
pumilus</em> producer and from the <em class=&quot;a-plus-plus&quot;>Escherichia
|
|
783
|
+
coli</em> BL21 recombinant strain bearing the pGEMGX1/ent/Bi plasmid.
|
|
784
|
+
Electrophoresis of all three samples of purified binase revealed two protein
|
|
785
|
+
fractions with ribonuclease activity possessing molecular masses of ∼12
|
|
786
|
+
and 25 kDa. The possible presence of binase II, a second secreted RNase, was
|
|
787
|
+
ruled out. Both high- and low-molecular mass proteins interacted with binase-specific
|
|
788
|
+
antibodies in the immunoblotting reaction, which indicated their antigenic
|
|
789
|
+
identity. The difference in molecular mass between these proteins indicated
|
|
790
|
+
the possible presence of two forms of binase in solution, a monomer and a
|
|
791
|
+
dimer.</p></description>\r\n<dc:identifier>http://link.springer.com/10.1134/S0026261715040177</dc:identifier>\r\n<dc:date>2015-07-01</dc:date>\n<dc:publisher>Springer-Verlag</dc:publisher>\n<prism:PublicationName>Microbiology</prism:PublicationName>\n<prism:publicationDate>2015-07-01</prism:publicationDate>\n<content:encoded><![CDATA[<p><a
|
|
792
|
+
href=\"http://link.springer.com/10.1134/S0026261715040177\"><b>Preparations
|
|
793
|
+
of Bacillus pumilus secreted RNase: One enzyme or two'</b></A><br /> <br
|
|
794
|
+
/><i>Microbiology, Vol. , No. (2015) pp. - </i><br /><h3 class=&quot;a-plus-plus&quot;>Abstract</h3>\n
|
|
795
|
+
\ <p class=&quot;a-plus-plus&quot;>Immunochemical
|
|
796
|
+
analysis of the following purified preparations of <em class=&quot;a-plus-plus&quot;>Bacillus
|
|
797
|
+
pumilus</em> RNase (binase) was carried out: industrially manufactured
|
|
798
|
+
enzyme (Institute of Organic Synthesis, Riga, Latvia) and the enzymes isolated
|
|
799
|
+
from the culture liquid of the native <em class=&quot;a-plus-plus&quot;>B.
|
|
800
|
+
pumilus</em> producer and from the <em class=&quot;a-plus-plus&quot;>Escherichia
|
|
801
|
+
coli</em> BL21 recombinant strain bearing the pGEMGX1/ent/Bi plasmid.
|
|
802
|
+
Electrophoresis of all three samples of purified binase revealed two protein
|
|
803
|
+
fractions with ribonuclease activity possessing molecular masses of ∼12
|
|
804
|
+
and 25 kDa. The possible presence of binase II, a second secreted RNase, was
|
|
805
|
+
ruled out. Both high- and low-molecular mass proteins interacted with binase-specific
|
|
806
|
+
antibodies in the immunoblotting reaction, which indicated their antigenic
|
|
807
|
+
identity. The difference in molecular mass between these proteins indicated
|
|
808
|
+
the possible presence of two forms of binase in solution, a monomer and a
|
|
809
|
+
dimer.</p></p>]]></content:encoded>\r\n</item>\n<item rdf:about=\"http://link.springer.com/10.1134/S0026261715040050\">\n<title>Metabolic
|
|
810
|
+
properties of Pachysolen tannophilus mutants producing xylitol and ethanol
|
|
811
|
+
from D-xylose</title>\r\n<link>http://link.springer.com/10.1134/S0026261715040050</link>\r\n<description><h3
|
|
812
|
+
class=&quot;a-plus-plus&quot;>Abstract</h3>\n <p
|
|
813
|
+
class=&quot;a-plus-plus&quot;>Activity of the major enzymes of
|
|
814
|
+
D-xylose metabolism in the mutants of xylose-utilizing yeasts <em class=&quot;a-plus-plus&quot;>Pachysolen
|
|
815
|
+
tannophilus</em> selectively producing xylitol or ethanol was studied.
|
|
816
|
+
The xylitol-producing strain exhibited low activities of xylitol dehydrogenase,
|
|
817
|
+
xylose reductase with preferential affinity to NADPH, NAD<sup class=&quot;a-plus-plus&quot;>+</sup>-dependent
|
|
818
|
+
malate dehydrogenase, and cytochrome <em class=&quot;a-plus-plus&quot;>c</em>
|
|
819
|
+
oxidase (4.40, 4.80, 1.87, and 0.28 μmol mg<sup class=&quot;a-plus-plus&quot;>−1</sup>
|
|
820
|
+
min<sup class=&quot;a-plus-plus&quot;>−1</sup>,
|
|
821
|
+
respectively). The cells of the ethanol-producing mutants exhibited elevated
|
|
822
|
+
activity of NADH/NADPH-xylose reductase, xylitol dehydrogenase, 1-glycerophosphate
|
|
823
|
+
dehydrogenase, and lactate dehydrogenase to 6.80, 8.60, 4.68, and 16.48 μmol
|
|
824
|
+
mg<sup class=&quot;a-plus-plus&quot;>−1</sup> min<sup
|
|
825
|
+
class=&quot;a-plus-plus&quot;>−1</sup>, respectively.
|
|
826
|
+
Effect of the NADPH/NADH imbalance on ethanol production accumulation and
|
|
827
|
+
xylitol accumulation is discussed.</p></description>\r\n<dc:identifier>http://link.springer.com/10.1134/S0026261715040050</dc:identifier>\r\n<dc:date>2015-07-01</dc:date>\n<dc:publisher>Springer-Verlag</dc:publisher>\n<prism:PublicationName>Microbiology</prism:PublicationName>\n<prism:publicationDate>2015-07-01</prism:publicationDate>\n<content:encoded><![CDATA[<p><a
|
|
828
|
+
href=\"http://link.springer.com/10.1134/S0026261715040050\"><b>Metabolic properties
|
|
829
|
+
of Pachysolen tannophilus mutants producing xylitol and ethanol from D-xylose</b></A><br
|
|
830
|
+
/> <br /><i>Microbiology, Vol. , No. (2015) pp. - </i><br /><h3 class=&quot;a-plus-plus&quot;>Abstract</h3>\n
|
|
831
|
+
\ <p class=&quot;a-plus-plus&quot;>Activity
|
|
832
|
+
of the major enzymes of D-xylose metabolism in the mutants of xylose-utilizing
|
|
833
|
+
yeasts <em class=&quot;a-plus-plus&quot;>Pachysolen tannophilus</em>
|
|
834
|
+
selectively producing xylitol or ethanol was studied. The xylitol-producing
|
|
835
|
+
strain exhibited low activities of xylitol dehydrogenase, xylose reductase
|
|
836
|
+
with preferential affinity to NADPH, NAD<sup class=&quot;a-plus-plus&quot;>+</sup>-dependent
|
|
837
|
+
malate dehydrogenase, and cytochrome <em class=&quot;a-plus-plus&quot;>c</em>
|
|
838
|
+
oxidase (4.40, 4.80, 1.87, and 0.28 μmol mg<sup class=&quot;a-plus-plus&quot;>−1</sup>
|
|
839
|
+
min<sup class=&quot;a-plus-plus&quot;>−1</sup>,
|
|
840
|
+
respectively). The cells of the ethanol-producing mutants exhibited elevated
|
|
841
|
+
activity of NADH/NADPH-xylose reductase, xylitol dehydrogenase, 1-glycerophosphate
|
|
842
|
+
dehydrogenase, and lactate dehydrogenase to 6.80, 8.60, 4.68, and 16.48 μmol
|
|
843
|
+
mg<sup class=&quot;a-plus-plus&quot;>−1</sup> min<sup
|
|
844
|
+
class=&quot;a-plus-plus&quot;>−1</sup>, respectively.
|
|
845
|
+
Effect of the NADPH/NADH imbalance on ethanol production accumulation and
|
|
846
|
+
xylitol accumulation is discussed.</p></p>]]></content:encoded>\r\n</item>\n<item
|
|
847
|
+
rdf:about=\"http://link.springer.com/10.1134/S0026261715040116\">\n<title>Transmembrane
|
|
848
|
+
adenylate cyclase controls the virulence factors of plant pathogenic Pseudomonas
|
|
849
|
+
siringae and mutualistic Rhizobium leguminosarum </title>\r\n<link>http://link.springer.com/10.1134/S0026261715040116</link>\r\n<description><h3
|
|
850
|
+
class=&quot;a-plus-plus&quot;>Abstract</h3>\n <p
|
|
851
|
+
class=&quot;a-plus-plus&quot;>The possible role of transmembrane
|
|
852
|
+
adenylate cyclase of a plant pathogen <em class=&quot;a-plus-plus&quot;>Pseudomonas
|
|
853
|
+
siringae</em> pv. <em class=&quot;a-plus-plus&quot;>pisi</em>
|
|
854
|
+
and of a symbiotroph <em class=&quot;a-plus-plus&quot;>Rhizobium
|
|
855
|
+
leguminosarum</em> bv. <em class=&quot;a-plus-plus&quot;>viceae</em>
|
|
856
|
+
in control of the activity of their virulence factors (cellulases and pectinases,
|
|
857
|
+
the enzymes degrading plant cell walls) was investigated. While transmembrane
|
|
858
|
+
adenylate cyclase was found to control the activity of virulence factors in
|
|
859
|
+
both pathogens and symbionts, the strategies employed by these microorganisms
|
|
860
|
+
in molecular dialogue with plants involving the adenylate cylcase signal system
|
|
861
|
+
exhibited both similarities and cardinal differences.</p></description>\r\n<dc:identifier>http://link.springer.com/10.1134/S0026261715040116</dc:identifier>\r\n<dc:date>2015-07-01</dc:date>\n<dc:publisher>Springer-Verlag</dc:publisher>\n<prism:PublicationName>Microbiology</prism:PublicationName>\n<prism:publicationDate>2015-07-01</prism:publicationDate>\n<content:encoded><![CDATA[<p><a
|
|
862
|
+
href=\"http://link.springer.com/10.1134/S0026261715040116\"><b>Transmembrane
|
|
863
|
+
adenylate cyclase controls the virulence factors of plant pathogenic Pseudomonas
|
|
864
|
+
siringae and mutualistic Rhizobium leguminosarum </b></A><br /> <br /><i>Microbiology,
|
|
865
|
+
Vol. , No. (2015) pp. - </i><br /><h3 class=&quot;a-plus-plus&quot;>Abstract</h3>\n
|
|
866
|
+
\ <p class=&quot;a-plus-plus&quot;>The possible
|
|
867
|
+
role of transmembrane adenylate cyclase of a plant pathogen <em class=&quot;a-plus-plus&quot;>Pseudomonas
|
|
868
|
+
siringae</em> pv. <em class=&quot;a-plus-plus&quot;>pisi</em>
|
|
869
|
+
and of a symbiotroph <em class=&quot;a-plus-plus&quot;>Rhizobium
|
|
870
|
+
leguminosarum</em> bv. <em class=&quot;a-plus-plus&quot;>viceae</em>
|
|
871
|
+
in control of the activity of their virulence factors (cellulases and pectinases,
|
|
872
|
+
the enzymes degrading plant cell walls) was investigated. While transmembrane
|
|
873
|
+
adenylate cyclase was found to control the activity of virulence factors in
|
|
874
|
+
both pathogens and symbionts, the strategies employed by these microorganisms
|
|
875
|
+
in molecular dialogue with plants involving the adenylate cylcase signal system
|
|
876
|
+
exhibited both similarities and cardinal differences.</p></p>]]></content:encoded>\r\n</item>\n<item
|
|
877
|
+
rdf:about=\"http://link.springer.com/10.1134/S0026261715040049\">\n<title>Production
|
|
878
|
+
of xylitol and ethanol and activity of the key enzymes of D-xylose consumption
|
|
879
|
+
in Pachysolen tannophilus mutant strains</title>\r\n<link>http://link.springer.com/10.1134/S0026261715040049</link>\r\n<description><h3
|
|
880
|
+
class=&quot;a-plus-plus&quot;>Abstract</h3>\n <p
|
|
881
|
+
class=&quot;a-plus-plus&quot;>Production of xylitol and ethanol,
|
|
882
|
+
as well as activities of the key enzymes of D-xylose consumption, were studied
|
|
883
|
+
in <em class=&quot;a-plus-plus&quot;>Pachysolen tannophilus</em>
|
|
884
|
+
mutants with altered growth on D-xylose, xylitol, ethanol, or D-glucose as
|
|
885
|
+
sole carbon sources. Suppressed activity of xylose reductase with preferential
|
|
886
|
+
affinity for NADPH and of xylitol dehydrogenase to 4.40 and 4.80 μmol
|
|
887
|
+
mg<sup class=&quot;a-plus-plus&quot;>−1</sup> min<sup
|
|
888
|
+
class=&quot;a-plus-plus&quot;>−1</sup>, respectively,
|
|
889
|
+
resulted in accumulation of xylitol (0.25 g per 1 g D-xylose consumed). The
|
|
890
|
+
highest levels of NADH/NADPH-xylose reductase and xylitol dehydrogenase (6.00–6.80
|
|
891
|
+
and 6.80–8.40 μmol mg<sup class=&quot;a-plus-plus&quot;>−1</sup>
|
|
892
|
+
min<sup class=&quot;a-plus-plus&quot;>−1</sup>,
|
|
893
|
+
respectively) were found in the strains producing 0.24–0.26 g ethanol
|
|
894
|
+
per 1 g D-xylose. Application of <em class=&quot;a-plus-plus&quot;>Pa.
|
|
895
|
+
tannophilus</em> mutants for analysis of the regulation of D-xylose
|
|
896
|
+
catabolism in yeasts is discussed.</p></description>\r\n<dc:identifier>http://link.springer.com/10.1134/S0026261715040049</dc:identifier>\r\n<dc:date>2015-07-01</dc:date>\n<dc:publisher>Springer-Verlag</dc:publisher>\n<prism:PublicationName>Microbiology</prism:PublicationName>\n<prism:publicationDate>2015-07-01</prism:publicationDate>\n<content:encoded><![CDATA[<p><a
|
|
897
|
+
href=\"http://link.springer.com/10.1134/S0026261715040049\"><b>Production
|
|
898
|
+
of xylitol and ethanol and activity of the key enzymes of D-xylose consumption
|
|
899
|
+
in Pachysolen tannophilus mutant strains</b></A><br /> <br /><i>Microbiology,
|
|
900
|
+
Vol. , No. (2015) pp. - </i><br /><h3 class=&quot;a-plus-plus&quot;>Abstract</h3>\n
|
|
901
|
+
\ <p class=&quot;a-plus-plus&quot;>Production
|
|
902
|
+
of xylitol and ethanol, as well as activities of the key enzymes of D-xylose
|
|
903
|
+
consumption, were studied in <em class=&quot;a-plus-plus&quot;>Pachysolen
|
|
904
|
+
tannophilus</em> mutants with altered growth on D-xylose, xylitol, ethanol,
|
|
905
|
+
or D-glucose as sole carbon sources. Suppressed activity of xylose reductase
|
|
906
|
+
with preferential affinity for NADPH and of xylitol dehydrogenase to 4.40
|
|
907
|
+
and 4.80 μmol mg<sup class=&quot;a-plus-plus&quot;>−1</sup>
|
|
908
|
+
min<sup class=&quot;a-plus-plus&quot;>−1</sup>,
|
|
909
|
+
respectively, resulted in accumulation of xylitol (0.25 g per 1 g D-xylose
|
|
910
|
+
consumed). The highest levels of NADH/NADPH-xylose reductase and xylitol dehydrogenase
|
|
911
|
+
(6.00–6.80 and 6.80–8.40 μmol mg<sup class=&quot;a-plus-plus&quot;>−1</sup>
|
|
912
|
+
min<sup class=&quot;a-plus-plus&quot;>−1</sup>,
|
|
913
|
+
respectively) were found in the strains producing 0.24–0.26 g ethanol
|
|
914
|
+
per 1 g D-xylose. Application of <em class=&quot;a-plus-plus&quot;>Pa.
|
|
915
|
+
tannophilus</em> mutants for analysis of the regulation of D-xylose
|
|
916
|
+
catabolism in yeasts is discussed.</p></p>]]></content:encoded>\r\n</item>\n\r\n\t\r\n
|
|
917
|
+
\ <rdf:Description rdf:ID=\"manifest\">\r\n <mn:channels>\r\n <rdf:Seq>\r\n
|
|
918
|
+
\ <rdf:li rdf:resource=\"http://www.journaltocs.hw.ac.uk/api/journals\"
|
|
919
|
+
/>\r\n </rdf:Seq>\r\n </mn:channels>\r\n </rdf:Description>\r\n\r\n</rdf:RDF>"
|
|
920
|
+
http_version:
|
|
921
|
+
recorded_at: Wed, 02 Sep 2015 20:57:47 GMT
|
|
922
|
+
recorded_with: VCR 2.9.3
|