spacr 0.0.2__py3-none-any.whl → 0.0.6__py3-none-any.whl
This diff represents the content of publicly available package versions that have been released to one of the supported registries. The information contained in this diff is provided for informational purposes only and reflects changes between package versions as they appear in their respective public registries.
- spacr/__init__.py +2 -2
- spacr/__main__.py +0 -2
- spacr/alpha.py +803 -14
- spacr/annotate_app.py +118 -120
- spacr/chris.py +50 -0
- spacr/core.py +1544 -533
- spacr/deep_spacr.py +696 -0
- spacr/foldseek.py +779 -0
- spacr/get_alfafold_structures.py +72 -0
- spacr/graph_learning.py +297 -253
- spacr/gui.py +145 -0
- spacr/gui_2.py +90 -0
- spacr/gui_classify_app.py +70 -80
- spacr/gui_mask_app.py +114 -91
- spacr/gui_measure_app.py +109 -88
- spacr/gui_utils.py +376 -32
- spacr/io.py +441 -438
- spacr/mask_app.py +116 -9
- spacr/measure.py +169 -69
- spacr/models/cp/toxo_pv_lumen.CP_model +0 -0
- spacr/old_code.py +70 -2
- spacr/plot.py +173 -17
- spacr/sequencing.py +1130 -0
- spacr/sim.py +630 -125
- spacr/timelapse.py +139 -10
- spacr/train.py +188 -21
- spacr/umap.py +0 -689
- spacr/utils.py +1360 -119
- {spacr-0.0.2.dist-info → spacr-0.0.6.dist-info}/METADATA +17 -29
- spacr-0.0.6.dist-info/RECORD +39 -0
- {spacr-0.0.2.dist-info → spacr-0.0.6.dist-info}/WHEEL +1 -1
- spacr-0.0.6.dist-info/entry_points.txt +9 -0
- spacr-0.0.2.dist-info/RECORD +0 -31
- spacr-0.0.2.dist-info/entry_points.txt +0 -7
- {spacr-0.0.2.dist-info → spacr-0.0.6.dist-info}/LICENSE +0 -0
- {spacr-0.0.2.dist-info → spacr-0.0.6.dist-info}/top_level.txt +0 -0
spacr/gui.py
ADDED
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import tkinter as tk
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from tkinter import ttk
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from tkinter import font as tkFont
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from PIL import Image, ImageTk
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import os
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import requests
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# Import your GUI apps
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from .gui_mask_app import initiate_mask_root
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from .gui_measure_app import initiate_measure_root
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from .annotate_app import initiate_annotation_app_root
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from .mask_app import initiate_mask_app_root
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from .gui_classify_app import initiate_classify_root
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from .gui_utils import CustomButton, style_text_boxes
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class MainApp(tk.Tk):
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def __init__(self):
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super().__init__()
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self.title("SpaCr GUI Collection")
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self.geometry("1100x1500")
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self.configure(bg="black")
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#self.attributes('-fullscreen', True)
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style = ttk.Style()
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style_text_boxes(style)
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self.gui_apps = {
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"Mask": (initiate_mask_root, "Generate cellpose masks for cells, nuclei and pathogen images."),
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"Measure": (initiate_measure_root, "Measure single object intensity and morphological feature. Crop and save single object image"),
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"Annotate": (initiate_annotation_app_root, "Annotation single object images on a grid. Annotations are saved to database."),
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"Make Masks": (initiate_mask_app_root, "Adjust pre-existing Cellpose models to your specific dataset for improved performance"),
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"Classify": (initiate_classify_root, "Train Torch Convolutional Neural Networks (CNNs) or Transformers to classify single object images.")
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}
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self.selected_app = tk.StringVar()
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self.create_widgets()
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def create_widgets(self):
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# Create the menu bar
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#create_menu_bar(self)
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# Create a canvas to hold the selected app and other elements
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self.canvas = tk.Canvas(self, bg="black", highlightthickness=0, width=4000, height=4000)
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self.canvas.grid(row=0, column=0, sticky="nsew")
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self.grid_rowconfigure(0, weight=1)
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self.grid_columnconfigure(0, weight=1)
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# Create a frame inside the canvas to hold the main content
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self.content_frame = tk.Frame(self.canvas, bg="black")
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self.content_frame.pack(fill=tk.BOTH, expand=True)
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# Create startup screen with buttons for each GUI app
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self.create_startup_screen()
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def create_startup_screen(self):
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self.clear_frame(self.content_frame)
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# Create a frame for the logo and description
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logo_frame = tk.Frame(self.content_frame, bg="black")
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logo_frame.pack(pady=20, expand=True)
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# Load the logo image
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if not self.load_logo(logo_frame):
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tk.Label(logo_frame, text="Logo not found", bg="black", fg="white", font=('Arial', 24, tkFont.NORMAL)).pack(padx=10, pady=10)
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# Add SpaCr text below the logo with padding for sharper text
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tk.Label(logo_frame, text="SpaCr", bg="black", fg="#008080", font=('Arial', 24, tkFont.NORMAL)).pack(padx=10, pady=10)
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# Create a frame for the buttons and descriptions
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buttons_frame = tk.Frame(self.content_frame, bg="black")
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buttons_frame.pack(pady=10, expand=True, padx=10)
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for i, (app_name, app_data) in enumerate(self.gui_apps.items()):
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app_func, app_desc = app_data
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# Create custom button with text
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button = CustomButton(buttons_frame, text=app_name, command=lambda app_name=app_name: self.load_app(app_name))
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button.grid(row=i, column=0, pady=10, padx=10, sticky="w")
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description_label = tk.Label(buttons_frame, text=app_desc, bg="black", fg="white", wraplength=800, justify="left", font=('Arial', 10, tkFont.NORMAL))
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description_label.grid(row=i, column=1, pady=10, padx=10, sticky="w")
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# Ensure buttons have a fixed width
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buttons_frame.grid_columnconfigure(0, minsize=150)
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# Ensure descriptions expand as needed
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buttons_frame.grid_columnconfigure(1, weight=1)
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def load_logo(self, frame):
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def download_image(url, save_path):
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try:
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response = requests.get(url, stream=True)
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response.raise_for_status() # Raise an HTTPError for bad responses
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with open(save_path, 'wb') as f:
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for chunk in response.iter_content(chunk_size=8192):
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f.write(chunk)
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return True
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except requests.exceptions.RequestException as e:
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print(f"Failed to download image from {url}: {e}")
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return False
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try:
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img_path = os.path.join(os.path.dirname(__file__), 'logo_spacr.png')
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print(f"Trying to load logo from {img_path}")
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logo_image = Image.open(img_path)
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except (FileNotFoundError, Image.UnidentifiedImageError):
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print(f"File {img_path} not found or is not a valid image. Attempting to download from GitHub.")
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if download_image('https://raw.githubusercontent.com/EinarOlafsson/spacr/main/spacr/logo_spacr.png', img_path):
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try:
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print(f"Downloaded file size: {os.path.getsize(img_path)} bytes")
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logo_image = Image.open(img_path)
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except Image.UnidentifiedImageError as e:
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print(f"Downloaded file is not a valid image: {e}")
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return False
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else:
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return False
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except Exception as e:
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print(f"An error occurred while loading the logo: {e}")
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return False
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try:
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logo_image = logo_image.resize((800, 800), Image.Resampling.LANCZOS)
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logo_photo = ImageTk.PhotoImage(logo_image)
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logo_label = tk.Label(frame, image=logo_photo, bg="black")
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logo_label.image = logo_photo # Keep a reference to avoid garbage collection
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logo_label.pack()
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return True
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except Exception as e:
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print(f"An error occurred while processing the logo image: {e}")
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return False
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def load_app(self, app_name):
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selected_app_func, _ = self.gui_apps[app_name]
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self.clear_frame(self.content_frame)
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app_frame = tk.Frame(self.content_frame, bg="black")
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app_frame.pack(fill=tk.BOTH, expand=True)
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selected_app_func(app_frame, self.winfo_width(), self.winfo_height())
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def clear_frame(self, frame):
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for widget in frame.winfo_children():
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widget.destroy()
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def gui_app():
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app = MainApp()
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app.mainloop()
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if __name__ == "__main__":
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gui_app()
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spacr/gui_2.py
ADDED
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import customtkinter as ctk
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from PIL import Image, ImageTk
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import os
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import requests
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class MainApp(ctk.CTk):
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def __init__(self):
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super().__init__()
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self.title("SpaCr GUI Collection")
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self.geometry("1200x800")
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ctk.set_appearance_mode("dark") # Modes: "System" (standard), "Dark", "Light"
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ctk.set_default_color_theme("dark-blue") # Themes: "blue" (standard), "green", "dark-blue")
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# Set scaling factor for high DPI displays; use a floating-point value.
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self.tk.call('tk', 'scaling', 1.5)
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self.create_widgets()
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def create_widgets(self):
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self.content_frame = ctk.CTkFrame(self)
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self.content_frame.pack(fill="both", expand=True, padx=20, pady=20)
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logo_frame = ctk.CTkFrame(self.content_frame)
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logo_frame.pack(pady=20, expand=True)
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if not self.load_logo(logo_frame):
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ctk.CTkLabel(logo_frame, text="Logo not found", text_color="white", font=('Helvetica', 24)).pack(padx=10, pady=10)
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ctk.CTkLabel(logo_frame, text="SpaCr", text_color="#00BFFF", font=('Helvetica', 36, "bold")).pack(padx=10, pady=10)
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button = ctk.CTkButton(
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self.content_frame,
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text="Mask",
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command=self.load_mask_app,
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width=250,
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height=60,
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corner_radius=20,
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fg_color="#1E90FF",
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hover_color="#4682B4",
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text_color="white",
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font=("Helvetica", 18, "bold")
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)
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button.pack(pady=20)
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def load_logo(self, frame):
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def download_image(url, save_path):
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try:
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response = requests.get(url, stream=True)
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response.raise_for_status()
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with open(save_path, 'wb') as f:
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for chunk in response.iter_content(chunk_size=8192):
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f.write(chunk)
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return True
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except requests.exceptions.RequestException as e:
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print(f"Failed to download image from {url}: {e}")
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return False
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try:
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img_path = os.path.join(os.path.dirname(__file__), 'logo_spacr.png')
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logo_image = Image.open(img_path)
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except (FileNotFoundError, Image.UnidentifiedImageError):
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if download_image('https://raw.githubusercontent.com/EinarOlafsson/spacr/main/spacr/logo_spacr.png', img_path):
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try:
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logo_image = Image.open(img_path)
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except Image.UnidentifiedImageError as e:
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return False
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else:
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return False
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except Exception as e:
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return False
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try:
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logo_image = logo_image.resize((200, 200), Image.Resampling.LANCZOS)
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logo_photo = ImageTk.PhotoImage(logo_image)
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logo_label = ctk.CTkLabel(frame, image=logo_photo)
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logo_label.image = logo_photo # Keep a reference to avoid garbage collection
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logo_label.pack()
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return True
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except Exception as e:
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return False
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def load_mask_app(self):
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print("Mask app loaded.") # Placeholder for mask app loading function
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def gui_app():
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app = MainApp()
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app.mainloop()
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if __name__ == "__main__":
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gui_app()
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spacr/gui_classify_app.py
CHANGED
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import sys, ctypes,
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import sys, ctypes, matplotlib
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import tkinter as tk
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from tkinter import ttk, scrolledtext
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from ttkthemes import ThemedTk
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from matplotlib.figure import Figure
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from matplotlib.backends.backend_tkagg import FigureCanvasTkAgg
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from matplotlib.figure import Figure
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pass
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from .logger import log_function_call
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from .gui_utils import ScrollableFrame, StdoutRedirector,
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from .gui_utils import classify_variables, check_classify_gui_settings, train_test_model_wrapper, read_settings_from_csv, update_settings_from_csv
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from .gui_utils import ScrollableFrame, StdoutRedirector, CustomButton, set_dark_style, set_default_font, generate_fields, process_stdout_stderr, clear_canvas, main_thread_update_function
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from .gui_utils import classify_variables, check_classify_gui_settings, train_test_model_wrapper, read_settings_from_csv, update_settings_from_csv, style_text_boxes, create_menu_bar
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thread_control = {"run_thread": None, "stop_requested": False}
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#@log_function_call
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def initiate_abort():
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global thread_control
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if thread_control.get("stop_requested") is not None:
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thread_control["run_thread"].terminate()
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thread_control["run_thread"] = None
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#@log_function_call
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def run_classify_gui(q, fig_queue, stop_requested):
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global vars_dict
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process_stdout_stderr(q)
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finally:
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stop_requested.value = 1
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#@log_function_call
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def start_process(q, fig_queue):
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global thread_control
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if thread_control.get("run_thread") is not None:
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new_settings = update_settings_from_csv(variables, csv_settings)
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vars_dict = generate_fields(new_settings, scrollable_frame)
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def initiate_classify_root(width, height):
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global
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theme = 'breeze'
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#@log_function_call
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def initiate_classify_root(parent_frame):#, width, height):
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global vars_dict, q, canvas, fig_queue, canvas_widget, thread_control
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set_dark_style(style)
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set_default_font(root, font_name="Arial", size=10)
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#root.state('zoomed') # For Windows to maximize the window
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root.attributes('-fullscreen', True)
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root.geometry(f"{width}x{height}")
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root.title("SpaCer: generate masks")
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style = ttk.Style(parent_frame)
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set_dark_style(style)
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+
style_text_boxes(style)
|
78
|
+
set_default_font(parent_frame, font_name="Arial", size=8)
|
79
|
+
|
80
|
+
parent_frame.configure(bg='#333333')
|
81
|
+
parent_frame.grid_rowconfigure(0, weight=1)
|
82
|
+
parent_frame.grid_columnconfigure(0, weight=1)
|
93
83
|
fig_queue = Queue()
|
94
|
-
|
84
|
+
|
95
85
|
def _process_fig_queue():
|
96
86
|
global canvas
|
97
87
|
try:
|
98
88
|
while not fig_queue.empty():
|
99
89
|
clear_canvas(canvas)
|
100
90
|
fig = fig_queue.get_nowait()
|
101
|
-
#set_fig_text_properties(fig, font_size=8)
|
102
91
|
for ax in fig.get_axes():
|
103
92
|
ax.set_xticks([]) # Remove x-axis ticks
|
104
93
|
ax.set_yticks([]) # Remove y-axis ticks
|
105
94
|
ax.xaxis.set_visible(False) # Hide the x-axis
|
106
95
|
ax.yaxis.set_visible(False) # Hide the y-axis
|
107
|
-
#ax.title.set_fontsize(14)
|
108
|
-
#disable_interactivity(fig)
|
109
96
|
fig.tight_layout()
|
110
97
|
fig.set_facecolor('#333333')
|
111
98
|
canvas.figure = fig
|
112
99
|
fig_width, fig_height = canvas_widget.winfo_width(), canvas_widget.winfo_height()
|
113
100
|
fig.set_size_inches(fig_width / fig.dpi, fig_height / fig.dpi, forward=True)
|
114
|
-
canvas.draw_idle()
|
101
|
+
canvas.draw_idle()
|
115
102
|
except Exception as e:
|
116
103
|
traceback.print_exc()
|
117
|
-
#pass
|
118
104
|
finally:
|
119
105
|
canvas_widget.after(100, _process_fig_queue)
|
120
|
-
|
121
|
-
# Process queue for console output
|
106
|
+
|
122
107
|
def _process_console_queue():
|
123
108
|
while not q.empty():
|
124
109
|
message = q.get_nowait()
|
125
110
|
console_output.insert(tk.END, message)
|
126
111
|
console_output.see(tk.END)
|
127
112
|
console_output.after(100, _process_console_queue)
|
128
|
-
|
129
|
-
# Vertical container for settings and console
|
130
|
-
vertical_container = tk.PanedWindow(root, orient=tk.HORIZONTAL) #VERTICAL
|
131
|
-
vertical_container.pack(fill=tk.BOTH, expand=True)
|
132
113
|
|
133
|
-
|
134
|
-
|
135
|
-
|
114
|
+
vertical_container = tk.PanedWindow(parent_frame, orient=tk.HORIZONTAL)
|
115
|
+
vertical_container.grid(row=0, column=0, sticky=tk.NSEW)
|
116
|
+
parent_frame.grid_rowconfigure(0, weight=1)
|
117
|
+
parent_frame.grid_columnconfigure(0, weight=1)
|
118
|
+
|
119
|
+
# Settings Section
|
120
|
+
settings_frame = tk.Frame(vertical_container, bg='#333333')
|
121
|
+
vertical_container.add(settings_frame, stretch="always")
|
122
|
+
settings_label = ttk.Label(settings_frame, text="Settings", background="#333333", foreground="white")
|
123
|
+
settings_label.grid(row=0, column=0, pady=10, padx=10)
|
124
|
+
scrollable_frame = ScrollableFrame(settings_frame, width=500)
|
125
|
+
scrollable_frame.grid(row=1, column=0, sticky="nsew")
|
126
|
+
settings_frame.grid_rowconfigure(1, weight=1)
|
127
|
+
settings_frame.grid_columnconfigure(0, weight=1)
|
136
128
|
|
137
129
|
# Setup for user input fields (variables)
|
138
130
|
variables = classify_variables()
|
139
131
|
vars_dict = generate_fields(variables, scrollable_frame)
|
140
|
-
|
141
|
-
# Horizontal container for Matplotlib figure and the vertical pane (for settings and console)
|
142
|
-
horizontal_container = tk.PanedWindow(vertical_container, orient=tk.VERTICAL) #HORIZONTAL
|
143
|
-
vertical_container.add(horizontal_container, stretch="always")
|
144
132
|
|
145
|
-
#
|
133
|
+
# Button section
|
134
|
+
import_btn = CustomButton(scrollable_frame.scrollable_frame, text="Import Settings", command=lambda: import_settings(scrollable_frame))
|
135
|
+
import_btn.grid(row=47, column=0, pady=20, padx=20)
|
136
|
+
run_button = CustomButton(scrollable_frame.scrollable_frame, text="Run", command=lambda: start_process(q, fig_queue))
|
137
|
+
run_button.grid(row=45, column=0, pady=20, padx=20)
|
138
|
+
abort_button = CustomButton(scrollable_frame.scrollable_frame, text="Abort", command=initiate_abort)
|
139
|
+
abort_button.grid(row=45, column=1, pady=20, padx=20)
|
140
|
+
progress_label = ttk.Label(scrollable_frame.scrollable_frame, text="Processing: 0%", background="black", foreground="white") # Create progress field
|
141
|
+
progress_label.grid(row=50, column=0, columnspan=2, sticky="ew", pady=(5, 0), padx=10)
|
142
|
+
|
143
|
+
# Plot Canvas Section
|
144
|
+
plot_frame = tk.PanedWindow(vertical_container, orient=tk.VERTICAL)
|
145
|
+
vertical_container.add(plot_frame, stretch="always")
|
146
146
|
figure = Figure(figsize=(30, 4), dpi=100, facecolor='#333333')
|
147
147
|
plot = figure.add_subplot(111)
|
148
|
-
plot.plot([], [])
|
148
|
+
plot.plot([], [])
|
149
149
|
plot.axis('off')
|
150
|
-
|
151
|
-
# Embedding the Matplotlib figure in the Tkinter window
|
152
|
-
canvas = FigureCanvasTkAgg(figure, master=horizontal_container)
|
150
|
+
canvas = FigureCanvasTkAgg(figure, master=plot_frame)
|
153
151
|
canvas.get_tk_widget().configure(cursor='arrow', background='#333333', highlightthickness=0)
|
154
|
-
#canvas.get_tk_widget().configure(cursor='arrow')
|
155
152
|
canvas_widget = canvas.get_tk_widget()
|
156
|
-
|
153
|
+
plot_frame.add(canvas_widget, stretch="always")
|
157
154
|
canvas.draw()
|
158
155
|
canvas.figure = figure
|
159
156
|
|
160
|
-
# Console
|
161
|
-
|
162
|
-
vertical_container.add(
|
157
|
+
# Console Section
|
158
|
+
console_frame = tk.Frame(vertical_container, bg='#333333')
|
159
|
+
vertical_container.add(console_frame, stretch="always")
|
160
|
+
console_label = ttk.Label(console_frame, text="Console", background="#333333", foreground="white")
|
161
|
+
console_label.grid(row=0, column=0, pady=10, padx=10)
|
162
|
+
console_output = scrolledtext.ScrolledText(console_frame, height=10, bg='#333333', fg='white', insertbackground='white')
|
163
|
+
console_output.grid(row=1, column=0, sticky="nsew")
|
164
|
+
console_frame.grid_rowconfigure(1, weight=1)
|
165
|
+
console_frame.grid_columnconfigure(0, weight=1)
|
163
166
|
|
164
|
-
# Queue and redirection setup for updating console output safely
|
165
167
|
q = Queue()
|
166
168
|
sys.stdout = StdoutRedirector(console_output)
|
167
169
|
sys.stderr = StdoutRedirector(console_output)
|
168
|
-
|
169
|
-
# This is your GUI setup where you create the Run button
|
170
|
-
run_button = ttk.Button(scrollable_frame.scrollable_frame, text="Run",command=lambda: start_process(q, fig_queue))
|
171
|
-
run_button.grid(row=40, column=0, pady=10)
|
172
|
-
|
173
|
-
abort_button = ttk.Button(scrollable_frame.scrollable_frame, text="Abort", command=initiate_abort)
|
174
|
-
abort_button.grid(row=40, column=1, pady=10)
|
175
|
-
|
176
|
-
progress_label = ttk.Label(scrollable_frame.scrollable_frame, text="Processing: 0%", background="#333333", foreground="white")
|
177
|
-
progress_label.grid(row=41, column=0, columnspan=2, sticky="ew", pady=(5, 0))
|
178
|
-
|
179
|
-
# Create the Import Settings button
|
180
|
-
import_btn = tk.Button(root, text="Import Settings", command=lambda: import_settings(scrollable_frame))
|
181
|
-
import_btn.pack(pady=20)
|
182
|
-
|
170
|
+
|
183
171
|
_process_console_queue()
|
184
172
|
_process_fig_queue()
|
185
|
-
|
186
|
-
|
187
|
-
|
188
|
-
|
189
|
-
return root, vars_dict
|
173
|
+
|
174
|
+
parent_frame.after(100, lambda: main_thread_update_function(parent_frame, q, fig_queue, canvas_widget, progress_label))
|
175
|
+
|
176
|
+
return parent_frame, vars_dict
|
190
177
|
|
191
178
|
def gui_classify():
|
192
|
-
|
193
|
-
root
|
179
|
+
root = tk.Tk()
|
180
|
+
root.geometry("1000x800")
|
181
|
+
root.title("SpaCer: generate masks")
|
182
|
+
initiate_classify_root(root, 1000, 800)
|
183
|
+
create_menu_bar(root)
|
194
184
|
root.mainloop()
|
195
185
|
|
196
186
|
if __name__ == "__main__":
|
197
|
-
gui_classify()
|
187
|
+
gui_classify()
|