phasorpy 0.7__cp312-cp312-manylinux2014_aarch64.manylinux_2_17_aarch64.manylinux_2_28_aarch64.whl

phasorpy/io/_ometiff.py

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+"""Read and write OME-TIFF file format."""
+
+from __future__ import annotations
+
+__all__ = [
+    # 'signal_from_ometiff',
+    'phasor_from_ometiff',
+    'phasor_to_ometiff',
+]
+
+import os
+import re
+from typing import TYPE_CHECKING
+
+from .._utils import parse_harmonic
+from ..utils import logger
+
+if TYPE_CHECKING:
+    from .._typing import (
+        Any,
+        ArrayLike,
+        DTypeLike,
+        Literal,
+        NDArray,
+        PathLike,
+        Sequence,
+    )
+
+import numpy
+
+from .. import __version__
+
+
+def phasor_to_ometiff(
+    filename: str | PathLike[Any],
+    mean: ArrayLike,
+    real: ArrayLike,
+    imag: ArrayLike,
+    /,
+    *,
+    frequency: float | None = None,
+    harmonic: int | Sequence[int] | None = None,
+    dims: Sequence[str] | None = None,
+    dtype: DTypeLike | None = None,
+    description: str | None = None,
+    **kwargs: Any,
+) -> None:
+    """Write phasor coordinate images and metadata to OME-TIFF file.
+
+    The OME-TIFF format is compatible with Bio-Formats and Fiji.
+
+    By default, write phasor coordinates as single precision floating point
+    values to separate image series.
+    Write images larger than (1024, 1024) pixels as (256, 256) tiles, datasets
+    larger than 2 GB as BigTIFF, and datasets larger than 8 KB using
+    zlib compression.
+
+    This file format is experimental and might be incompatible with future
+    versions of this library. It is intended for temporarily exchanging
+    phasor coordinates with other software, not as a long-term storage
+    solution.
+
+    Parameters
+    ----------
+    filename : str or Path
+        Name of PhasorPy OME-TIFF file to write.
+    mean : array_like
+        Average intensity image. Write to an image series named 'Phasor mean'.
+    real : array_like
+        Image of real component of phasor coordinates.
+        Multiple harmonics, if any, must be in the first dimension.
+        Write to image series named 'Phasor real'.
+    imag : array_like
+        Image of imaginary component of phasor coordinates.
+        Multiple harmonics, if any, must be in the first dimension.
+        Write to image series named 'Phasor imag'.
+    frequency : float, optional
+        Fundamental frequency of time-resolved phasor coordinates.
+        Usually in units of MHz.
+        Write to image series named 'Phasor frequency'.
+    harmonic : int or sequence of int, optional
+        Harmonics present in the first dimension of `real` and `imag`, if any.
+        Write to image series named 'Phasor harmonic'.
+        Only needed if harmonics are not starting at and increasing by one.
+    dims : sequence of str, optional
+        Character codes for `mean` image dimensions.
+        By default, the last dimensions are assumed to be 'TZCYX'.
+        If harmonics are present in `real` and `imag`, an "other" (``Q``)
+        dimension is prepended to axes for those arrays.
+        Refer to the OME-TIFF model for allowed axes and their order.
+    dtype : dtype_like, optional
+        Floating point data type used to store phasor coordinates.
+        The default is ``float32``, which has 6 digits of precision
+        and maximizes compatibility with other software.
+    description : str, optional
+        Plain-text description of dataset. Write as OME dataset description.
+    **kwargs
+        Additional arguments passed to :py:class:`tifffile.TiffWriter` and
+        :py:meth:`tifffile.TiffWriter.write`.
+        For example, ``compression=None`` writes image data uncompressed.
+
+    See Also
+    --------
+    phasorpy.io.phasor_from_ometiff
+
+    Notes
+    -----
+    Scalar or one-dimensional phasor coordinate arrays are written as images.
+
+    The OME-TIFF format is specified in the
+    `OME Data Model and File Formats Documentation
+    <https://ome-model.readthedocs.io/>`_.
+
+    The `6D, 7D and 8D storage
+    <https://ome-model.readthedocs.io/en/latest/developers/6d-7d-and-8d-storage.html>`_
+    extension is used to store multi-harmonic phasor coordinates.
+    The modulo type for the first, harmonic dimension is "other".
+
+    The implementation is based on the
+    `tifffile <https://github.com/cgohlke/tifffile/>`__ library.
+
+    Examples
+    --------
+    >>> mean, real, imag = numpy.random.rand(3, 32, 32, 32)
+    >>> phasor_to_ometiff(
+    ...     '_phasorpy.ome.tif', mean, real, imag, dims='ZYX', frequency=80.0
+    ... )
+
+    """
+    import tifffile
+
+    if dtype is None:
+        dtype = numpy.float32
+    dtype = numpy.dtype(dtype)
+    if dtype.kind != 'f':
+        raise ValueError(f'{dtype=} not a floating point type')
+
+    mean = numpy.asarray(mean, dtype)
+    real = numpy.asarray(real, dtype)
+    imag = numpy.asarray(imag, dtype)
+    datasize = mean.nbytes + real.nbytes + imag.nbytes
+
+    if real.shape != imag.shape:
+        raise ValueError(f'{real.shape=} != {imag.shape=}')
+    if mean.shape != real.shape[-mean.ndim :]:
+        raise ValueError(f'{mean.shape=} != {real.shape[-mean.ndim:]=}')
+    has_harmonic_dim = real.ndim == mean.ndim + 1
+    if mean.ndim == real.ndim or real.ndim == 0:
+        nharmonic = 1
+    else:
+        nharmonic = real.shape[0]
+
+    if mean.ndim < 2:
+        # not an image
+        mean = mean.reshape(1, -1)
+        if has_harmonic_dim:
+            real = real.reshape(real.shape[0], 1, -1)
+            imag = imag.reshape(imag.shape[0], 1, -1)
+        else:
+            real = real.reshape(1, -1)
+            imag = imag.reshape(1, -1)
+
+    if harmonic is not None:
+        harmonic, _ = parse_harmonic(harmonic)
+        if len(harmonic) != nharmonic:
+            raise ValueError('invalid harmonic')
+
+    if frequency is not None:
+        frequency_array = numpy.atleast_2d(frequency).astype(numpy.float64)
+        if frequency_array.size > 1:
+            raise ValueError('frequency must be scalar')
+
+    axes = 'TZCYX'[-mean.ndim :] if dims is None else ''.join(tuple(dims))
+    if len(axes) != mean.ndim:
+        raise ValueError(f'{axes=} does not match {mean.ndim=}')
+    axes_phasor = axes if mean.ndim == real.ndim else 'Q' + axes
+
+    if 'photometric' not in kwargs:
+        kwargs['photometric'] = 'minisblack'
+    if 'compression' not in kwargs and datasize > 8192:
+        kwargs['compression'] = 'zlib'
+    if 'tile' not in kwargs and 'rowsperstrip' not in kwargs:
+        if (
+            axes.endswith('YX')
+            and mean.shape[-1] > 1024
+            and mean.shape[-2] > 1024
+        ):
+            kwargs['tile'] = (256, 256)
+
+    mode = kwargs.pop('mode', None)
+    bigtiff = kwargs.pop('bigtiff', None)
+    if bigtiff is None:
+        bigtiff = datasize > 2**31
+
+    metadata = kwargs.pop('metadata', {})
+    if 'Creator' not in metadata:
+        metadata['Creator'] = f'PhasorPy {__version__}'
+
+    dataset = metadata.pop('Dataset', {})
+    if 'Name' not in dataset:
+        dataset['Name'] = 'Phasor'
+    if description:
+        dataset['Description'] = description
+    metadata['Dataset'] = dataset
+
+    if has_harmonic_dim:
+        metadata['TypeDescription'] = {'Q': 'Phasor harmonics'}
+
+    with tifffile.TiffWriter(
+        filename, bigtiff=bigtiff, mode=mode, ome=True
+    ) as tif:
+        metadata['Name'] = 'Phasor mean'
+        metadata['axes'] = axes
+        tif.write(mean, metadata=metadata, **kwargs)
+        del metadata['Dataset']
+
+        metadata['Name'] = 'Phasor real'
+        metadata['axes'] = axes_phasor
+        tif.write(real, metadata=metadata, **kwargs)
+
+        metadata['Name'] = 'Phasor imag'
+        tif.write(imag, metadata=metadata, **kwargs)
+
+        if frequency is not None:
+            tif.write(frequency_array, metadata={'Name': 'Phasor frequency'})
+
+        if harmonic is not None:
+            tif.write(
+                numpy.atleast_2d(harmonic).astype(numpy.uint32),
+                metadata={'Name': 'Phasor harmonic'},
+            )
+
+
+def phasor_from_ometiff(
+    filename: str | PathLike[Any],
+    /,
+    *,
+    harmonic: int | Sequence[int] | Literal['all'] | str | None = None,
+) -> tuple[NDArray[Any], NDArray[Any], NDArray[Any], dict[str, Any]]:
+    """Return phasor coordinates and metadata from PhasorPy OME-TIFF file.
+
+    PhasorPy OME-TIFF files contain phasor mean intensity, real and imaginary
+    components, along with frequency and harmonic information.
+
+    Parameters
+    ----------
+    filename : str or Path
+        Name of PhasorPy OME-TIFF file to read.
+    harmonic : int, sequence of int, or 'all', optional
+        Harmonic(s) to return from file.
+        If None (default), return the first harmonic stored in the file.
+        If `'all'`, return all harmonics as stored in file.
+        If a list, the first axes of the returned `real` and `imag` arrays
+        contain specified harmonic(s).
+        If an integer, the returned `real` and `imag` arrays are single
+        harmonic and have the same shape as `mean`.
+
+    Returns
+    -------
+    mean : ndarray
+        Average intensity image.
+    real : ndarray
+        Image of real component of phasor coordinates.
+    imag : ndarray
+        Image of imaginary component of phasor coordinates.
+    attrs : dict
+        Select metadata:
+
+        - ``'dims'`` (tuple of str):
+          :ref:`Axes codes <axes>` for `mean` image dimensions.
+        - ``'harmonic'`` (int or list of int):
+          Harmonic(s) present in `real` and `imag`.
+          If a scalar, `real` and `imag` are single harmonic and contain no
+          harmonic axes.
+          If a list, `real` and `imag` contain one or more harmonics in the
+          first axis.
+        - ``'frequency'`` (float, optional):
+          Fundamental frequency of time-resolved phasor coordinates.
+          Usually in units of MHz.
+        - ``'description'`` (str, optional):
+          OME dataset plain-text description.
+
+    Raises
+    ------
+    tifffile.TiffFileError
+        File is not a TIFF file.
+    ValueError
+        File is not an OME-TIFF containing phasor coordinates.
+    IndexError
+        Harmonic is not found in file.
+
+    See Also
+    --------
+    phasorpy.io.phasor_to_ometiff
+
+    Notes
+    -----
+    Scalar or one-dimensional phasor coordinates stored in the file are
+    returned as two-dimensional images (three-dimensional if multiple
+    harmonics are present).
+
+    The implementation is based on the
+    `tifffile <https://github.com/cgohlke/tifffile/>`__ library.
+
+    Examples
+    --------
+    >>> mean, real, imag = numpy.random.rand(3, 32, 32, 32)
+    >>> phasor_to_ometiff(
+    ...     '_phasorpy.ome.tif', mean, real, imag, dims='ZYX', frequency=80.0
+    ... )
+    >>> mean, real, imag, attrs = phasor_from_ometiff('_phasorpy.ome.tif')
+    >>> mean
+    array(...)
+    >>> mean.dtype
+    dtype('float32')
+    >>> mean.shape
+    (32, 32, 32)
+    >>> attrs['dims']
+    ('Z', 'Y', 'X')
+    >>> attrs['frequency']
+    80.0
+    >>> attrs['harmonic']
+    1
+
+    """
+    import tifffile
+
+    name = os.path.basename(filename)
+
+    with tifffile.TiffFile(filename) as tif:
+        if (
+            not tif.is_ome
+            or len(tif.series) < 3
+            or tif.series[0].name != 'Phasor mean'
+            or tif.series[1].name != 'Phasor real'
+            or tif.series[2].name != 'Phasor imag'
+        ):
+            raise ValueError(
+                f'{name!r} is not an OME-TIFF containing phasor images'
+            )
+
+        attrs: dict[str, Any] = {'dims': tuple(tif.series[0].axes)}
+
+        # TODO: read coords from OME-XML
+        ome_xml = tif.ome_metadata
+        assert ome_xml is not None
+
+        # TODO: parse OME-XML
+        match = re.search(
+            r'><Description>(.*)</Description><',
+            ome_xml,
+            re.MULTILINE | re.DOTALL,
+        )
+        if match is not None:
+            attrs['description'] = (
+                match.group(1)
+                .replace('&amp;', '&')
+                .replace('&gt;', '>')
+                .replace('&lt;', '<')
+            )
+
+        has_harmonic_dim = tif.series[1].ndim > tif.series[0].ndim
+        nharmonics = tif.series[1].shape[0] if has_harmonic_dim else 1
+        harmonic_max = nharmonics
+        for i in (3, 4):
+            if len(tif.series) < i + 1:
+                break
+            series = tif.series[i]
+            data = series.asarray().squeeze()
+            if series.name == 'Phasor frequency':
+                attrs['frequency'] = float(data.item(0))
+            elif series.name == 'Phasor harmonic':
+                if not has_harmonic_dim and data.size == 1:
+                    attrs['harmonic'] = int(data.item(0))
+                    harmonic_max = attrs['harmonic']
+                elif has_harmonic_dim and data.size == nharmonics:
+                    attrs['harmonic'] = data.tolist()
+                    harmonic_max = max(attrs['harmonic'])
+                else:
+                    logger().warning(
+                        f'harmonic={data} does not match phasor '
+                        f'shape={tif.series[1].shape}'
+                    )
+
+        if 'harmonic' not in attrs:
+            if has_harmonic_dim:
+                attrs['harmonic'] = list(range(1, nharmonics + 1))
+            else:
+                attrs['harmonic'] = 1
+        harmonic_stored = attrs['harmonic']
+
+        mean = tif.series[0].asarray()
+        if harmonic is None:
+            # first harmonic in file
+            if isinstance(harmonic_stored, list):
+                attrs['harmonic'] = harmonic_stored[0]
+            else:
+                attrs['harmonic'] = harmonic_stored
+            real = tif.series[1].asarray()
+            if has_harmonic_dim:
+                real = real[0].copy()
+            imag = tif.series[2].asarray()
+            if has_harmonic_dim:
+                imag = imag[0].copy()
+        elif isinstance(harmonic, str) and harmonic == 'all':
+            # all harmonics as stored in file
+            real = tif.series[1].asarray()
+            imag = tif.series[2].asarray()
+        else:
+            # specified harmonics
+            harmonic, keepdims = parse_harmonic(harmonic, harmonic_max)
+            try:
+                if isinstance(harmonic_stored, list):
+                    index = [harmonic_stored.index(h) for h in harmonic]
+                else:
+                    index = [[harmonic_stored].index(h) for h in harmonic]
+            except ValueError as exc:
+                raise IndexError('harmonic not found') from exc
+
+            if has_harmonic_dim:
+                if keepdims:
+                    attrs['harmonic'] = [harmonic_stored[i] for i in index]
+                    real = tif.series[1].asarray()[index].copy()
+                    imag = tif.series[2].asarray()[index].copy()
+                else:
+                    attrs['harmonic'] = harmonic_stored[index[0]]
+                    real = tif.series[1].asarray()[index[0]].copy()
+                    imag = tif.series[2].asarray()[index[0]].copy()
+            elif keepdims:
+                real = tif.series[1].asarray()
+                real = real.reshape(1, *real.shape)
+                imag = tif.series[2].asarray()
+                imag = imag.reshape(1, *imag.shape)
+                attrs['harmonic'] = [harmonic_stored]
+            else:
+                real = tif.series[1].asarray()
+                imag = tif.series[2].asarray()
+
+    if real.shape != imag.shape:
+        logger().warning(f'{real.shape=} != {imag.shape=}')
+    if real.shape[-mean.ndim :] != mean.shape:
+        logger().warning(f'{real.shape[-mean.ndim:]=} != {mean.shape=}')
+
+    return mean, real, imag, attrs