celldetective 1.4.2__py3-none-any.whl → 1.5.0b0__py3-none-any.whl

celldetective/relative_measurements.py

@@ -1,468 +1,677 @@
 import pandas as pd
 import numpy as np
-from celldetective.utils import derivative, extract_identity_col
+from celldetective.utils.maths import derivative
+from celldetective.utils.data_cleaning import extract_identity_col
 import os
 import subprocess
-abs_path = os.sep.join([os.path.split(os.path.dirname(os.path.realpath(__file__)))[0], 'celldetective'])
+
+abs_path = os.sep.join(
+    [os.path.split(os.path.dirname(os.path.realpath(__file__)))[0], "celldetective"]
+)
+
 
 def measure_pairs(pos, neighborhood_protocol):
-	
-	reference_population = neighborhood_protocol['reference']
-	neighbor_population = neighborhood_protocol['neighbor']
-	neighborhood_type = neighborhood_protocol['type']
-	neighborhood_distance = neighborhood_protocol['distance']
-	neighborhood_description = neighborhood_protocol['description']
-
-	relative_measurements = []
-
-	tab_ref = pos + os.sep.join(['output', 'tables', f'trajectories_{reference_population}.pkl'])
-	if os.path.exists(tab_ref):
-		df_reference = np.load(tab_ref, allow_pickle=True)
-	else:
-		df_reference = None
-
-	if os.path.exists(tab_ref.replace(reference_population, neighbor_population)):
-		df_neighbor = np.load(tab_ref.replace(reference_population, neighbor_population), allow_pickle=True)
-	else:
-		if os.path.exists(tab_ref.replace(reference_population, neighbor_population).replace('.pkl','.csv')):
-			df_neighbor = pd.read_csv(tab_ref.replace(reference_population, neighbor_population).replace('.pkl','.csv'))
-		else:
-			df_neighbor = None
-
-	if df_reference is None:
-		return None
-
-	assert str(neighborhood_description) in list(df_reference.columns)
-	neighborhood = df_reference.loc[:,f'{neighborhood_description}'].to_numpy()
-
-	ref_id_col = extract_identity_col(df_reference)
-	ref_tracked = False
-	if ref_id_col is None:
-		return None
-	elif ref_id_col=='TRACK_ID':
-		ref_tracked = True
-	neigh_id_col = extract_identity_col(df_neighbor)
-	neigh_tracked = False
-	if neigh_id_col is None:
-		return None
-	elif neigh_id_col=='TRACK_ID':
-		neigh_tracked = True
-	
-	centre_of_mass_columns = [(c,c.replace('POSITION_X','POSITION_Y')) for c in list(df_neighbor.columns) if c.endswith('centre_of_mass_POSITION_X')]
-	centre_of_mass_labels = [c.replace('_centre_of_mass_POSITION_X','') for c in list(df_neighbor.columns) if c.endswith('centre_of_mass_POSITION_X')]
-
-	for t in np.unique(list(df_reference['FRAME'].unique())+list(df_neighbor['FRAME'])):
-		
-		group_reference = df_reference.loc[df_reference['FRAME']==t,:]
-		group_neighbors = df_neighbor.loc[df_neighbor['FRAME']==t, :]
-		
-		for tid, group in group_reference.groupby(ref_id_col):
-
-			neighborhood = group.loc[: , f'{neighborhood_description}'].to_numpy()[0]
-			coords_reference = group[['POSITION_X', 'POSITION_Y']].to_numpy()[0]
-
-			neighbors = []
-			if isinstance(neighborhood, float) or neighborhood!=neighborhood:
-				pass
-			else:
-				for neigh in neighborhood:
-					neighbors.append(neigh['id'])
-
-			unique_neigh = list(np.unique(neighbors))
-			print(f'{unique_neigh=}')
-
-			neighbor_properties = group_neighbors.loc[group_neighbors[neigh_id_col].isin(unique_neigh)]
-			
-			for nc, group_neigh in neighbor_properties.groupby(neigh_id_col):
-				
-				neighbor_vector = np.zeros((2))
-				neighbor_vector[:] = np.nan
-				mass_displacement_vector = np.zeros((len(centre_of_mass_columns), 2))
-
-				coords_centre_of_mass = []
-				for col in centre_of_mass_columns:
-					coords_centre_of_mass.append(group_neigh[[col[0],col[1]]].to_numpy()[0])
-
-				dot_product_vector = np.zeros((len(centre_of_mass_columns)))
-				dot_product_vector[:] = np.nan
-
-				cosine_dot_vector = np.zeros((len(centre_of_mass_columns)))
-				cosine_dot_vector[:] = np.nan
-
-				coords_neighbor = group_neigh[['POSITION_X', 'POSITION_Y']].to_numpy()[0]
-				intersection = np.nan
-				if 'intersection' in list(group_neigh.columns):
-					intersection = group_neigh['intersection'].values[0]
-
-				neighbor_vector[0] = coords_neighbor[0] - coords_reference[0]
-				neighbor_vector[1] = coords_neighbor[1] - coords_reference[1]
-
-				if neighbor_vector[0]==neighbor_vector[0] and neighbor_vector[1]==neighbor_vector[1]:
-					angle = np.arctan2(neighbor_vector[1], neighbor_vector[0])
-					relative_distance = np.sqrt(neighbor_vector[0]**2 + neighbor_vector[1]**2)
-					
-					for z,cols in enumerate(centre_of_mass_columns):
-
-							mass_displacement_vector[z,0] = coords_centre_of_mass[z][0] - coords_neighbor[0]
-							mass_displacement_vector[z,1] = coords_centre_of_mass[z][1] - coords_neighbor[1]
-
-							dot_product_vector[z] = np.dot(mass_displacement_vector[z], -neighbor_vector)
-							cosine_dot_vector[z] = np.dot(mass_displacement_vector[z], -neighbor_vector) / (np.linalg.norm(mass_displacement_vector[z])*np.linalg.norm(-neighbor_vector))
-
-					relative_measurements.append(
-							{'REFERENCE_ID': tid, 'NEIGHBOR_ID': nc,
-							'reference_population': reference_population,
-							'neighbor_population': neighbor_population,
-							'FRAME': t, 'distance': relative_distance, 'intersection': intersection,
-							'angle': angle * 180 / np.pi,
-							f'status_{neighborhood_description}': 1,
-							f'class_{neighborhood_description}': 0,
-							'reference_tracked': ref_tracked, 'neighbors_tracked': neigh_tracked,
-							 })
-					for z,lbl in enumerate(centre_of_mass_labels):
-						relative_measurements[-1].update({lbl+'_centre_of_mass_dot_product': dot_product_vector[z], lbl+'_centre_of_mass_dot_cosine': cosine_dot_vector[z]})
-	
-	df_pairs = pd.DataFrame(relative_measurements)
-	
-	return df_pairs
-
-
-
-def measure_pair_signals_at_position(pos, neighborhood_protocol, velocity_kwargs={'window': 3, 'mode': 'bi'}):
-
-
-	reference_population = neighborhood_protocol['reference']
-	neighbor_population = neighborhood_protocol['neighbor']
-	neighborhood_type = neighborhood_protocol['type']
-	neighborhood_distance = neighborhood_protocol['distance']
-	neighborhood_description = neighborhood_protocol['description']
-
-	relative_measurements = []
-
-	tab_ref = pos + os.sep.join(['output', 'tables', f'trajectories_{reference_population}.pkl'])
-	if os.path.exists(tab_ref):
-		df_reference = np.load(tab_ref, allow_pickle=True)
-	else:
-		df_reference = None
-
-	if os.path.exists(tab_ref.replace(reference_population, neighbor_population)):
-		df_neighbor = np.load(tab_ref.replace(reference_population, neighbor_population), allow_pickle=True)
-	else:
-		if os.path.exists(tab_ref.replace(reference_population, neighbor_population).replace('.pkl','.csv')):
-			df_neighbor = pd.read_csv(tab_ref.replace(reference_population, neighbor_population).replace('.pkl','.csv'))
-		else:
-			df_neighbor = None
-
-	if df_reference is None:
-		return None
-
-	assert str(neighborhood_description) in list(df_reference.columns)
-	neighborhood = df_reference.loc[:,f'{neighborhood_description}'].to_numpy()
-
-	ref_id_col = extract_identity_col(df_reference)
-	if ref_id_col is not None:
-		df_reference = df_reference.sort_values(by=[ref_id_col, 'FRAME'])
-
-	ref_tracked = False
-	if ref_id_col=='TRACK_ID':
-		compute_velocity = True
-		ref_tracked = True
-	elif ref_id_col=='ID':
-		df_pairs = measure_pairs(pos, neighborhood_protocol)
-		return df_pairs
-	else:
-		print('ID or TRACK ID column could not be found in neighbor table. Abort.')
-		return None
-
-	print(f'Measuring pair signals...')
-
-	neigh_id_col = extract_identity_col(df_neighbor)
-	neigh_tracked = False
-	if neigh_id_col=='TRACK_ID':
-		compute_velocity = True
-		neigh_tracked = True
-	elif neigh_id_col=='ID':
-		df_pairs = measure_pairs(pos, neighborhood_protocol)
-		return df_pairs
-	else:
-		print('ID or TRACK ID column could not be found in neighbor table. Abort.')
-		return None
-
-	try:
-		for tid, group in df_reference.groupby(ref_id_col):
-
-			neighbor_dicts = group.loc[: , f'{neighborhood_description}'].values
-			timeline_reference = group['FRAME'].to_numpy()
-			coords_reference = group[['POSITION_X', 'POSITION_Y']].to_numpy()
-			if "area" in list(group.columns):
-				ref_area = group['area'].to_numpy()
-			else:
-				ref_area = [np.nan]*len(coords_reference)
-
-			neighbor_ids = []
-			neighbor_ids_per_t = []
-			intersection_values = []
-
-			time_of_first_entrance_in_neighborhood = {}
-			t_departure={}
-
-			for t in range(len(timeline_reference)):
-
-				neighbors_at_t = neighbor_dicts[t]
-				neighs_t = []
-				if isinstance(neighbors_at_t, float) or neighbors_at_t!=neighbors_at_t:
-					pass
-				else:
-					for neigh in neighbors_at_t:
-						if neigh['id'] not in neighbor_ids:
-							time_of_first_entrance_in_neighborhood[neigh['id']]=t
-						if 'intersection' in neigh:
-							intersection_values.append({"frame": t, "neigh_id": neigh['id'], "intersection": neigh['intersection']})
-						else:
-							intersection_values.append({"frame": t, "neigh_id": neigh['id'], "intersection": np.nan})
-						neighbor_ids.append(neigh['id'])
-						neighs_t.append(neigh['id'])
-				neighbor_ids_per_t.append(neighs_t)
-
-			intersection_values = pd.DataFrame(intersection_values)
-
-			#print(neighbor_ids_per_t)
-			unique_neigh = list(np.unique(neighbor_ids))
-			print(f'Reference cell {tid}: found {len(unique_neigh)} neighbour cells: {unique_neigh}...')
-
-			neighbor_properties = df_neighbor.loc[df_neighbor[neigh_id_col].isin(unique_neigh)]
-
-			for nc, group_neigh in neighbor_properties.groupby(neigh_id_col):
-				
-				coords_neighbor = group_neigh[['POSITION_X', 'POSITION_Y']].to_numpy()
-				timeline_neighbor = group_neigh['FRAME'].to_numpy()
-				if "area" in list(group_neigh.columns):
-					neigh_area = group_neigh['area'].to_numpy()
-				else:
-					neigh_area = [np.nan]*len(timeline_neighbor)
-
-
-				# # Perform timeline matching to have same start-end points and no gaps
-				full_timeline, _, _ = timeline_matching(timeline_reference, timeline_neighbor)
-
-				neighbor_vector = np.zeros((len(full_timeline), 2))
-				neighbor_vector[:,:] = np.nan
-
-				intersection_vector = np.zeros((len(full_timeline)))
-				intersection_vector[:] = np.nan
-
-				centre_of_mass_columns = [(c,c.replace('POSITION_X','POSITION_Y')) for c in list(neighbor_properties.columns) if c.endswith('centre_of_mass_POSITION_X')]
-				centre_of_mass_labels = [c.replace('_centre_of_mass_POSITION_X','') for c in list(neighbor_properties.columns) if c.endswith('centre_of_mass_POSITION_X')]
-
-				mass_displacement_vector = np.zeros((len(centre_of_mass_columns), len(full_timeline), 2))
-				mass_displacement_vector[:,:,:] = np.nan
-
-				dot_product_vector = np.zeros((len(centre_of_mass_columns), len(full_timeline)))
-				dot_product_vector[:,:] = np.nan
-
-				cosine_dot_vector = np.zeros((len(centre_of_mass_columns), len(full_timeline)))
-				cosine_dot_vector[:,:] = np.nan
-
-				coords_centre_of_mass = []
-				for col in centre_of_mass_columns:
-					coords_centre_of_mass.append(group_neigh[[col[0],col[1]]].to_numpy())
-
-				# Relative distance
-				for t in range(len(full_timeline)):
-
-					if t in timeline_reference and t in timeline_neighbor: # meaning position exists on both sides
-
-						idx_reference = list(timeline_reference).index(t) #index_reference[list(full_timeline).index(t)]
-						idx_neighbor = list(timeline_neighbor).index(t) #index_neighbor[list(full_timeline).index(t)]
-
-						neighbor_vector[t, 0] = coords_neighbor[idx_neighbor, 0] - coords_reference[idx_reference, 0]
-						neighbor_vector[t, 1] = coords_neighbor[idx_neighbor, 1] - coords_reference[idx_reference, 1]
-
-						for z,cols in enumerate(centre_of_mass_columns):
-
-							mass_displacement_vector[z,t,0] = coords_centre_of_mass[z][idx_neighbor, 0] - coords_neighbor[idx_neighbor, 0]
-							mass_displacement_vector[z,t,1] = coords_centre_of_mass[z][idx_neighbor, 1] - coords_neighbor[idx_neighbor, 1]
-
-							dot_product_vector[z,t] = np.dot(mass_displacement_vector[z,t], -neighbor_vector[t])
-							cosine_dot_vector[z,t] = np.dot(mass_displacement_vector[z,t], -neighbor_vector[t]) / (np.linalg.norm(mass_displacement_vector[z,t])*np.linalg.norm(-neighbor_vector[t]))
-							if tid==44.0 and nc==173.0:
-								print(f'{centre_of_mass_columns[z]=} {mass_displacement_vector[z,t]=} {-neighbor_vector[t]=} {dot_product_vector[z,t]=} {cosine_dot_vector[z,t]=}')
-							
-
-				angle = np.zeros(len(full_timeline))
-				angle[:] = np.nan
-
-				exclude = neighbor_vector[:,1]!=neighbor_vector[:,1]
-				angle[~exclude] = np.arctan2(neighbor_vector[:, 1][~exclude], neighbor_vector[:, 0][~exclude])
-				#print(f'Angle before unwrap: {angle}')
-				angle[~exclude] = np.unwrap(angle[~exclude])
-				#print(f'Angle after unwrap: {angle}')
-				relative_distance = np.sqrt(neighbor_vector[:,0]**2 + neighbor_vector[:, 1]**2)
-				#print(f'Timeline: {full_timeline}; Distance: {relative_distance}')
-
-				if compute_velocity:
-					rel_velocity = derivative(relative_distance, full_timeline, **velocity_kwargs)
-					rel_velocity_long_timescale = derivative(relative_distance, full_timeline, window = 7, mode='bi')
-					#rel_velocity = np.insert(rel_velocity, 0, np.nan)[:-1]
-					
-					angular_velocity = np.zeros(len(full_timeline))
-					angular_velocity[:] = np.nan
-					angular_velocity_long_timescale = np.zeros(len(full_timeline))
-					angular_velocity_long_timescale[:] = np.nan
-
-					angular_velocity[~exclude] = derivative(angle[~exclude], full_timeline[~exclude], **velocity_kwargs)
-					angular_velocity_long_timescale[~exclude] = derivative(angle[~exclude], full_timeline[~exclude], window = 7, mode='bi')
-
-				# 	angular_velocity = np.zeros(len(full_timeline))
-				# 	angular_velocity[:] = np.nan
-
-				# 	for t in range(1, len(relative_angle1)):
-				# 		if not np.isnan(relative_angle1[t]) and not np.isnan(relative_angle1[t - 1]):
-				# 			delta_angle = relative_angle1[t] - relative_angle1[t - 1]
-				# 			delta_time = full_timeline[t] - full_timeline[t - 1]
-				# 			if delta_time != 0:
-				# 				angular_velocity[t] = delta_angle / delta_time
-
-				duration_in_neigh = list(neighbor_ids).count(nc)
-				#print(nc, duration_in_neigh, ' frames')
-
-				cum_sum = 0
-				for t in range(len(full_timeline)):
-
-					if t in timeline_reference and t in timeline_neighbor: # meaning position exists on both sides
-
-						idx_reference = list(timeline_reference).index(t)
-						idx_neighbor = list(timeline_neighbor).index(t)
-						inter = intersection_values.loc[(intersection_values['neigh_id']==nc)&(intersection_values["frame"]==t),"intersection"].values
-						if len(inter)==0:
-							inter = np.nan
-						else:
-							inter = inter[0]
-
-						neigh_inter_fraction = np.nan
-						if inter==inter and neigh_area[idx_neighbor]==neigh_area[idx_neighbor]:
-							neigh_inter_fraction = inter / neigh_area[idx_neighbor]
-
-						ref_inter_fraction = np.nan
-						if inter==inter and ref_area[idx_reference]==ref_area[idx_reference]:
-							ref_inter_fraction = inter / ref_area[idx_reference]						
-
-						if nc in neighbor_ids_per_t[idx_reference]:
-
-							cum_sum+=1
-							relative_measurements.append(
-									{'REFERENCE_ID': tid, 'NEIGHBOR_ID': nc,
-									'reference_population': reference_population,
-									'neighbor_population': neighbor_population,
-									'FRAME': t, 'distance': relative_distance[t], 'intersection': inter, 'reference_frac_area_intersection': ref_inter_fraction, 'neighbor_frac_area_intersection': neigh_inter_fraction,
-									'velocity': rel_velocity[t],
-									'velocity_smooth': rel_velocity_long_timescale[t], 
-									'angle': angle[t] * 180 / np.pi,
-									#'angle-neigh-ref': angle[t] * 180 / np.pi, 
-									'angular_velocity': angular_velocity[t],
-									'angular_velocity_smooth': angular_velocity_long_timescale[t],
-									f'status_{neighborhood_description}': 1,
-									f'residence_time_in_{neighborhood_description}': cum_sum,
-									f'class_{neighborhood_description}': 0,
-									f't0_{neighborhood_description}': time_of_first_entrance_in_neighborhood[nc],
-									'reference_tracked': ref_tracked, 'neighbors_tracked': neigh_tracked,
-									 })
-							for z,lbl in enumerate(centre_of_mass_labels):
-								relative_measurements[-1].update({lbl+'_centre_of_mass_dot_product': dot_product_vector[z,t], lbl+'_centre_of_mass_dot_cosine': cosine_dot_vector[z,t]})
-
-						else:
-							relative_measurements.append(
-									{'REFERENCE_ID': tid, 'NEIGHBOR_ID': nc,
-									'reference_population': reference_population,
-									'neighbor_population': neighbor_population,
-									'FRAME': t, 'distance': relative_distance[t], 'intersection': inter, 'reference_frac_area_intersection': ref_inter_fraction, 'neighbor_frac_area_intersection': neigh_inter_fraction,
-									'velocity': rel_velocity[t], 
-									'velocity_smooth': rel_velocity_long_timescale[t],
-									'angle': angle[t] * 180 / np.pi,
-									#'angle-neigh-ref': angle[t] * 180 / np.pi, 
-									'angular_velocity': angular_velocity[t],
-									'angular_velocity_smooth': angular_velocity_long_timescale[t],
-									f'status_{neighborhood_description}': 0,
-									f'residence_time_in_{neighborhood_description}': cum_sum,
-									f'class_{neighborhood_description}': 0,
-									f't0_{neighborhood_description}': time_of_first_entrance_in_neighborhood[nc],
-									'reference_tracked': ref_tracked, 'neighbors_tracked': neigh_tracked,
-									 })
-							for z,lbl in enumerate(centre_of_mass_labels):
-								relative_measurements[-1].update({lbl+'_centre_of_mass_dot_product': dot_product_vector[z,t], lbl+'_centre_of_mass_dot_cosine': cosine_dot_vector[z,t]})
-
-		df_pairs = pd.DataFrame(relative_measurements)
-
-		return df_pairs
-
-	except KeyError:
-		print(f"Neighborhood not found in data frame. Measurements for this neighborhood will not be calculated")
 
+    reference_population = neighborhood_protocol["reference"]
+    neighbor_population = neighborhood_protocol["neighbor"]
+    neighborhood_type = neighborhood_protocol["type"]
+    neighborhood_distance = neighborhood_protocol["distance"]
+    neighborhood_description = neighborhood_protocol["description"]
+
+    relative_measurements = []
+
+    tab_ref = pos + os.sep.join(
+        ["output", "tables", f"trajectories_{reference_population}.pkl"]
+    )
+    if os.path.exists(tab_ref):
+        df_reference = np.load(tab_ref, allow_pickle=True)
+    else:
+        df_reference = None
+
+    if os.path.exists(tab_ref.replace(reference_population, neighbor_population)):
+        df_neighbor = np.load(
+            tab_ref.replace(reference_population, neighbor_population),
+            allow_pickle=True,
+        )
+    else:
+        if os.path.exists(
+            tab_ref.replace(reference_population, neighbor_population).replace(
+                ".pkl", ".csv"
+            )
+        ):
+            df_neighbor = pd.read_csv(
+                tab_ref.replace(reference_population, neighbor_population).replace(
+                    ".pkl", ".csv"
+                )
+            )
+        else:
+            df_neighbor = None
+
+    if df_reference is None:
+        return None
+
+    assert str(neighborhood_description) in list(df_reference.columns)
+    neighborhood = df_reference.loc[:, f"{neighborhood_description}"].to_numpy()
+
+    ref_id_col = extract_identity_col(df_reference)
+    ref_tracked = False
+    if ref_id_col is None:
+        return None
+    elif ref_id_col == "TRACK_ID":
+        ref_tracked = True
+    neigh_id_col = extract_identity_col(df_neighbor)
+    neigh_tracked = False
+    if neigh_id_col is None:
+        return None
+    elif neigh_id_col == "TRACK_ID":
+        neigh_tracked = True
+
+    centre_of_mass_columns = [
+        (c, c.replace("POSITION_X", "POSITION_Y"))
+        for c in list(df_neighbor.columns)
+        if c.endswith("centre_of_mass_POSITION_X")
+    ]
+    centre_of_mass_labels = [
+        c.replace("_centre_of_mass_POSITION_X", "")
+        for c in list(df_neighbor.columns)
+        if c.endswith("centre_of_mass_POSITION_X")
+    ]
+
+    for t in np.unique(
+        list(df_reference["FRAME"].unique()) + list(df_neighbor["FRAME"])
+    ):
+
+        group_reference = df_reference.loc[df_reference["FRAME"] == t, :]
+        group_neighbors = df_neighbor.loc[df_neighbor["FRAME"] == t, :]
+
+        for tid, group in group_reference.groupby(ref_id_col):
+
+            neighborhood = group.loc[:, f"{neighborhood_description}"].to_numpy()[0]
+            coords_reference = group[["POSITION_X", "POSITION_Y"]].to_numpy()[0]
+
+            neighbors = []
+            if isinstance(neighborhood, float) or neighborhood != neighborhood:
+                pass
+            else:
+                for neigh in neighborhood:
+                    neighbors.append(neigh["id"])
+
+            unique_neigh = list(np.unique(neighbors))
+            print(f"{unique_neigh=}")
+
+            neighbor_properties = group_neighbors.loc[
+                group_neighbors[neigh_id_col].isin(unique_neigh)
+            ]
+
+            for nc, group_neigh in neighbor_properties.groupby(neigh_id_col):
+
+                neighbor_vector = np.zeros((2))
+                neighbor_vector[:] = np.nan
+                mass_displacement_vector = np.zeros((len(centre_of_mass_columns), 2))
+
+                coords_centre_of_mass = []
+                for col in centre_of_mass_columns:
+                    coords_centre_of_mass.append(
+                        group_neigh[[col[0], col[1]]].to_numpy()[0]
+                    )
+
+                dot_product_vector = np.zeros((len(centre_of_mass_columns)))
+                dot_product_vector[:] = np.nan
+
+                cosine_dot_vector = np.zeros((len(centre_of_mass_columns)))
+                cosine_dot_vector[:] = np.nan
+
+                coords_neighbor = group_neigh[["POSITION_X", "POSITION_Y"]].to_numpy()[
+                    0
+                ]
+                intersection = np.nan
+                if "intersection" in list(group_neigh.columns):
+                    intersection = group_neigh["intersection"].values[0]
+
+                neighbor_vector[0] = coords_neighbor[0] - coords_reference[0]
+                neighbor_vector[1] = coords_neighbor[1] - coords_reference[1]
+
+                if (
+                    neighbor_vector[0] == neighbor_vector[0]
+                    and neighbor_vector[1] == neighbor_vector[1]
+                ):
+                    angle = np.arctan2(neighbor_vector[1], neighbor_vector[0])
+                    relative_distance = np.sqrt(
+                        neighbor_vector[0] ** 2 + neighbor_vector[1] ** 2
+                    )
+
+                    for z, cols in enumerate(centre_of_mass_columns):
+
+                        mass_displacement_vector[z, 0] = (
+                            coords_centre_of_mass[z][0] - coords_neighbor[0]
+                        )
+                        mass_displacement_vector[z, 1] = (
+                            coords_centre_of_mass[z][1] - coords_neighbor[1]
+                        )
+
+                        dot_product_vector[z] = np.dot(
+                            mass_displacement_vector[z], -neighbor_vector
+                        )
+                        cosine_dot_vector[z] = np.dot(
+                            mass_displacement_vector[z], -neighbor_vector
+                        ) / (
+                            np.linalg.norm(mass_displacement_vector[z])
+                            * np.linalg.norm(-neighbor_vector)
+                        )
+
+                    relative_measurements.append(
+                        {
+                            "REFERENCE_ID": tid,
+                            "NEIGHBOR_ID": nc,
+                            "reference_population": reference_population,
+                            "neighbor_population": neighbor_population,
+                            "FRAME": t,
+                            "distance": relative_distance,
+                            "intersection": intersection,
+                            "angle": angle * 180 / np.pi,
+                            f"status_{neighborhood_description}": 1,
+                            f"class_{neighborhood_description}": 0,
+                            "reference_tracked": ref_tracked,
+                            "neighbors_tracked": neigh_tracked,
+                        }
+                    )
+                    for z, lbl in enumerate(centre_of_mass_labels):
+                        relative_measurements[-1].update(
+                            {
+                                lbl
+                                + "_centre_of_mass_dot_product": dot_product_vector[z],
+                                lbl
+                                + "_centre_of_mass_dot_cosine": cosine_dot_vector[z],
+                            }
+                        )
+
+    df_pairs = pd.DataFrame(relative_measurements)
+
+    return df_pairs
+
+
+def measure_pair_signals_at_position(
+    pos, neighborhood_protocol, velocity_kwargs={"window": 3, "mode": "bi"}
+):
+
+    reference_population = neighborhood_protocol["reference"]
+    neighbor_population = neighborhood_protocol["neighbor"]
+    neighborhood_type = neighborhood_protocol["type"]
+    neighborhood_distance = neighborhood_protocol["distance"]
+    neighborhood_description = neighborhood_protocol["description"]
+
+    relative_measurements = []
+
+    tab_ref = pos + os.sep.join(
+        ["output", "tables", f"trajectories_{reference_population}.pkl"]
+    )
+    if os.path.exists(tab_ref):
+        df_reference = np.load(tab_ref, allow_pickle=True)
+    else:
+        df_reference = None
+
+    if os.path.exists(tab_ref.replace(reference_population, neighbor_population)):
+        df_neighbor = np.load(
+            tab_ref.replace(reference_population, neighbor_population),
+            allow_pickle=True,
+        )
+    else:
+        if os.path.exists(
+            tab_ref.replace(reference_population, neighbor_population).replace(
+                ".pkl", ".csv"
+            )
+        ):
+            df_neighbor = pd.read_csv(
+                tab_ref.replace(reference_population, neighbor_population).replace(
+                    ".pkl", ".csv"
+                )
+            )
+        else:
+            df_neighbor = None
+
+    if df_reference is None:
+        return None
+
+    assert str(neighborhood_description) in list(df_reference.columns)
+    neighborhood = df_reference.loc[:, f"{neighborhood_description}"].to_numpy()
+
+    ref_id_col = extract_identity_col(df_reference)
+    if ref_id_col is not None:
+        df_reference = df_reference.sort_values(by=[ref_id_col, "FRAME"])
+
+    ref_tracked = False
+    if ref_id_col == "TRACK_ID":
+        compute_velocity = True
+        ref_tracked = True
+    elif ref_id_col == "ID":
+        df_pairs = measure_pairs(pos, neighborhood_protocol)
+        return df_pairs
+    else:
+        print("ID or TRACK ID column could not be found in neighbor table. Abort.")
+        return None
+
+    print(f"Measuring pair signals...")
+
+    neigh_id_col = extract_identity_col(df_neighbor)
+    neigh_tracked = False
+    if neigh_id_col == "TRACK_ID":
+        compute_velocity = True
+        neigh_tracked = True
+    elif neigh_id_col == "ID":
+        df_pairs = measure_pairs(pos, neighborhood_protocol)
+        return df_pairs
+    else:
+        print("ID or TRACK ID column could not be found in neighbor table. Abort.")
+        return None
+
+    try:
+        for tid, group in df_reference.groupby(ref_id_col):
+
+            neighbor_dicts = group.loc[:, f"{neighborhood_description}"].values
+            timeline_reference = group["FRAME"].to_numpy()
+            coords_reference = group[["POSITION_X", "POSITION_Y"]].to_numpy()
+            if "area" in list(group.columns):
+                ref_area = group["area"].to_numpy()
+            else:
+                ref_area = [np.nan] * len(coords_reference)
+
+            neighbor_ids = []
+            neighbor_ids_per_t = []
+            intersection_values = []
+
+            time_of_first_entrance_in_neighborhood = {}
+            t_departure = {}
+
+            for t in range(len(timeline_reference)):
+
+                neighbors_at_t = neighbor_dicts[t]
+                neighs_t = []
+                if (
+                    isinstance(neighbors_at_t, float)
+                    or neighbors_at_t != neighbors_at_t
+                ):
+                    pass
+                else:
+                    for neigh in neighbors_at_t:
+                        if neigh["id"] not in neighbor_ids:
+                            time_of_first_entrance_in_neighborhood[neigh["id"]] = t
+                        if "intersection" in neigh:
+                            intersection_values.append(
+                                {
+                                    "frame": t,
+                                    "neigh_id": neigh["id"],
+                                    "intersection": neigh["intersection"],
+                                }
+                            )
+                        else:
+                            intersection_values.append(
+                                {
+                                    "frame": t,
+                                    "neigh_id": neigh["id"],
+                                    "intersection": np.nan,
+                                }
+                            )
+                        neighbor_ids.append(neigh["id"])
+                        neighs_t.append(neigh["id"])
+                neighbor_ids_per_t.append(neighs_t)
+
+            intersection_values = pd.DataFrame(intersection_values)
+
+            # print(neighbor_ids_per_t)
+            unique_neigh = list(np.unique(neighbor_ids))
+            print(
+                f"Reference cell {tid}: found {len(unique_neigh)} neighbour cells: {unique_neigh}..."
+            )
+
+            neighbor_properties = df_neighbor.loc[
+                df_neighbor[neigh_id_col].isin(unique_neigh)
+            ]
+
+            for nc, group_neigh in neighbor_properties.groupby(neigh_id_col):
+
+                coords_neighbor = group_neigh[["POSITION_X", "POSITION_Y"]].to_numpy()
+                timeline_neighbor = group_neigh["FRAME"].to_numpy()
+                if "area" in list(group_neigh.columns):
+                    neigh_area = group_neigh["area"].to_numpy()
+                else:
+                    neigh_area = [np.nan] * len(timeline_neighbor)
+
+                # # Perform timeline matching to have same start-end points and no gaps
+                full_timeline, _, _ = timeline_matching(
+                    timeline_reference, timeline_neighbor
+                )
+
+                neighbor_vector = np.zeros((len(full_timeline), 2))
+                neighbor_vector[:, :] = np.nan
+
+                intersection_vector = np.zeros((len(full_timeline)))
+                intersection_vector[:] = np.nan
+
+                centre_of_mass_columns = [
+                    (c, c.replace("POSITION_X", "POSITION_Y"))
+                    for c in list(neighbor_properties.columns)
+                    if c.endswith("centre_of_mass_POSITION_X")
+                ]
+                centre_of_mass_labels = [
+                    c.replace("_centre_of_mass_POSITION_X", "")
+                    for c in list(neighbor_properties.columns)
+                    if c.endswith("centre_of_mass_POSITION_X")
+                ]
+
+                mass_displacement_vector = np.zeros(
+                    (len(centre_of_mass_columns), len(full_timeline), 2)
+                )
+                mass_displacement_vector[:, :, :] = np.nan
+
+                dot_product_vector = np.zeros(
+                    (len(centre_of_mass_columns), len(full_timeline))
+                )
+                dot_product_vector[:, :] = np.nan
+
+                cosine_dot_vector = np.zeros(
+                    (len(centre_of_mass_columns), len(full_timeline))
+                )
+                cosine_dot_vector[:, :] = np.nan
+
+                coords_centre_of_mass = []
+                for col in centre_of_mass_columns:
+                    coords_centre_of_mass.append(
+                        group_neigh[[col[0], col[1]]].to_numpy()
+                    )
+
+                # Relative distance
+                for t in range(len(full_timeline)):
+
+                    if (
+                        t in timeline_reference and t in timeline_neighbor
+                    ):  # meaning position exists on both sides
+
+                        idx_reference = list(timeline_reference).index(
+                            t
+                        )  # index_reference[list(full_timeline).index(t)]
+                        idx_neighbor = list(timeline_neighbor).index(
+                            t
+                        )  # index_neighbor[list(full_timeline).index(t)]
+
+                        neighbor_vector[t, 0] = (
+                            coords_neighbor[idx_neighbor, 0]
+                            - coords_reference[idx_reference, 0]
+                        )
+                        neighbor_vector[t, 1] = (
+                            coords_neighbor[idx_neighbor, 1]
+                            - coords_reference[idx_reference, 1]
+                        )
+
+                        for z, cols in enumerate(centre_of_mass_columns):
+
+                            mass_displacement_vector[z, t, 0] = (
+                                coords_centre_of_mass[z][idx_neighbor, 0]
+                                - coords_neighbor[idx_neighbor, 0]
+                            )
+                            mass_displacement_vector[z, t, 1] = (
+                                coords_centre_of_mass[z][idx_neighbor, 1]
+                                - coords_neighbor[idx_neighbor, 1]
+                            )
+
+                            dot_product_vector[z, t] = np.dot(
+                                mass_displacement_vector[z, t], -neighbor_vector[t]
+                            )
+                            cosine_dot_vector[z, t] = np.dot(
+                                mass_displacement_vector[z, t], -neighbor_vector[t]
+                            ) / (
+                                np.linalg.norm(mass_displacement_vector[z, t])
+                                * np.linalg.norm(-neighbor_vector[t])
+                            )
+                            if tid == 44.0 and nc == 173.0:
+                                print(
+                                    f"{centre_of_mass_columns[z]=} {mass_displacement_vector[z,t]=} {-neighbor_vector[t]=} {dot_product_vector[z,t]=} {cosine_dot_vector[z,t]=}"
+                                )
+
+                angle = np.zeros(len(full_timeline))
+                angle[:] = np.nan
+
+                exclude = neighbor_vector[:, 1] != neighbor_vector[:, 1]
+                angle[~exclude] = np.arctan2(
+                    neighbor_vector[:, 1][~exclude], neighbor_vector[:, 0][~exclude]
+                )
+                # print(f'Angle before unwrap: {angle}')
+                angle[~exclude] = np.unwrap(angle[~exclude])
+                # print(f'Angle after unwrap: {angle}')
+                relative_distance = np.sqrt(
+                    neighbor_vector[:, 0] ** 2 + neighbor_vector[:, 1] ** 2
+                )
+                # print(f'Timeline: {full_timeline}; Distance: {relative_distance}')
+
+                if compute_velocity:
+                    rel_velocity = derivative(
+                        relative_distance, full_timeline, **velocity_kwargs
+                    )
+                    rel_velocity_long_timescale = derivative(
+                        relative_distance, full_timeline, window=7, mode="bi"
+                    )
+                    # rel_velocity = np.insert(rel_velocity, 0, np.nan)[:-1]
+
+                    angular_velocity = np.zeros(len(full_timeline))
+                    angular_velocity[:] = np.nan
+                    angular_velocity_long_timescale = np.zeros(len(full_timeline))
+                    angular_velocity_long_timescale[:] = np.nan
+
+                    angular_velocity[~exclude] = derivative(
+                        angle[~exclude], full_timeline[~exclude], **velocity_kwargs
+                    )
+                    angular_velocity_long_timescale[~exclude] = derivative(
+                        angle[~exclude], full_timeline[~exclude], window=7, mode="bi"
+                    )
+
+                # 	angular_velocity = np.zeros(len(full_timeline))
+                # 	angular_velocity[:] = np.nan
+
+                # 	for t in range(1, len(relative_angle1)):
+                # 		if not np.isnan(relative_angle1[t]) and not np.isnan(relative_angle1[t - 1]):
+                # 			delta_angle = relative_angle1[t] - relative_angle1[t - 1]
+                # 			delta_time = full_timeline[t] - full_timeline[t - 1]
+                # 			if delta_time != 0:
+                # 				angular_velocity[t] = delta_angle / delta_time
+
+                duration_in_neigh = list(neighbor_ids).count(nc)
+                # print(nc, duration_in_neigh, ' frames')
+
+                cum_sum = 0
+                for t in range(len(full_timeline)):
+
+                    if (
+                        t in timeline_reference and t in timeline_neighbor
+                    ):  # meaning position exists on both sides
+
+                        idx_reference = list(timeline_reference).index(t)
+                        idx_neighbor = list(timeline_neighbor).index(t)
+                        inter = intersection_values.loc[
+                            (intersection_values["neigh_id"] == nc)
+                            & (intersection_values["frame"] == t),
+                            "intersection",
+                        ].values
+                        if len(inter) == 0:
+                            inter = np.nan
+                        else:
+                            inter = inter[0]
+
+                        neigh_inter_fraction = np.nan
+                        if (
+                            inter == inter
+                            and neigh_area[idx_neighbor] == neigh_area[idx_neighbor]
+                        ):
+                            neigh_inter_fraction = inter / neigh_area[idx_neighbor]
+
+                        ref_inter_fraction = np.nan
+                        if (
+                            inter == inter
+                            and ref_area[idx_reference] == ref_area[idx_reference]
+                        ):
+                            ref_inter_fraction = inter / ref_area[idx_reference]
+
+                        if nc in neighbor_ids_per_t[idx_reference]:
+
+                            cum_sum += 1
+                            relative_measurements.append(
+                                {
+                                    "REFERENCE_ID": tid,
+                                    "NEIGHBOR_ID": nc,
+                                    "reference_population": reference_population,
+                                    "neighbor_population": neighbor_population,
+                                    "FRAME": t,
+                                    "distance": relative_distance[t],
+                                    "intersection": inter,
+                                    "reference_frac_area_intersection": ref_inter_fraction,
+                                    "neighbor_frac_area_intersection": neigh_inter_fraction,
+                                    "velocity": rel_velocity[t],
+                                    "velocity_smooth": rel_velocity_long_timescale[t],
+                                    "angle": angle[t] * 180 / np.pi,
+                                    #'angle-neigh-ref': angle[t] * 180 / np.pi,
+                                    "angular_velocity": angular_velocity[t],
+                                    "angular_velocity_smooth": angular_velocity_long_timescale[
+                                        t
+                                    ],
+                                    f"status_{neighborhood_description}": 1,
+                                    f"residence_time_in_{neighborhood_description}": cum_sum,
+                                    f"class_{neighborhood_description}": 0,
+                                    f"t0_{neighborhood_description}": time_of_first_entrance_in_neighborhood[
+                                        nc
+                                    ],
+                                    "reference_tracked": ref_tracked,
+                                    "neighbors_tracked": neigh_tracked,
+                                }
+                            )
+                            for z, lbl in enumerate(centre_of_mass_labels):
+                                relative_measurements[-1].update(
+                                    {
+                                        lbl
+                                        + "_centre_of_mass_dot_product": dot_product_vector[
+                                            z, t
+                                        ],
+                                        lbl
+                                        + "_centre_of_mass_dot_cosine": cosine_dot_vector[
+                                            z, t
+                                        ],
+                                    }
+                                )
+
+                        else:
+                            relative_measurements.append(
+                                {
+                                    "REFERENCE_ID": tid,
+                                    "NEIGHBOR_ID": nc,
+                                    "reference_population": reference_population,
+                                    "neighbor_population": neighbor_population,
+                                    "FRAME": t,
+                                    "distance": relative_distance[t],
+                                    "intersection": inter,
+                                    "reference_frac_area_intersection": ref_inter_fraction,
+                                    "neighbor_frac_area_intersection": neigh_inter_fraction,
+                                    "velocity": rel_velocity[t],
+                                    "velocity_smooth": rel_velocity_long_timescale[t],
+                                    "angle": angle[t] * 180 / np.pi,
+                                    #'angle-neigh-ref': angle[t] * 180 / np.pi,
+                                    "angular_velocity": angular_velocity[t],
+                                    "angular_velocity_smooth": angular_velocity_long_timescale[
+                                        t
+                                    ],
+                                    f"status_{neighborhood_description}": 0,
+                                    f"residence_time_in_{neighborhood_description}": cum_sum,
+                                    f"class_{neighborhood_description}": 0,
+                                    f"t0_{neighborhood_description}": time_of_first_entrance_in_neighborhood[
+                                        nc
+                                    ],
+                                    "reference_tracked": ref_tracked,
+                                    "neighbors_tracked": neigh_tracked,
+                                }
+                            )
+                            for z, lbl in enumerate(centre_of_mass_labels):
+                                relative_measurements[-1].update(
+                                    {
+                                        lbl
+                                        + "_centre_of_mass_dot_product": dot_product_vector[
+                                            z, t
+                                        ],
+                                        lbl
+                                        + "_centre_of_mass_dot_cosine": cosine_dot_vector[
+                                            z, t
+                                        ],
+                                    }
+                                )
+
+        df_pairs = pd.DataFrame(relative_measurements)
+
+        return df_pairs
+
+    except KeyError:
+        print(
+            f"Neighborhood not found in data frame. Measurements for this neighborhood will not be calculated"
+        )
 
-def timeline_matching(timeline1, timeline2):
 
-	"""
-	Match two timelines and create a unified timeline with corresponding indices.
-
-	Parameters
-	----------
-	timeline1 : array-like
-		The first timeline to be matched.
-	timeline2 : array-like
-		The second timeline to be matched.
-
-	Returns
-	-------
-	tuple
-		A tuple containing:
-
-			- full_timeline : numpy.ndarray
-				The unified timeline spanning from the minimum to the maximum time point in the input timelines.
-			- index1 : list of int
-				The indices of `timeline1` in the `full_timeline`.
-			- index2 : list of int
-				The indices of `timeline2` in the `full_timeline`.
-
-	Examples
-	--------
-	>>> timeline1 = [1, 2, 5, 6]
-	>>> timeline2 = [2, 3, 4, 6]
-	>>> full_timeline, index1, index2 = timeline_matching(timeline1, timeline2)
-	>>> print(full_timeline)
-	[1 2 3 4 5 6]
-	>>> print(index1)
-	[0, 1, 4, 5]
-	>>> print(index2)
-	[1, 2, 3, 5]
-
-	Notes
-	-----
-	- The function combines the two timelines and generates a continuous range from the minimum to the maximum time point.
-	- It then finds the indices of the original timelines in this unified timeline.
-	- The function assumes that the input timelines consist of integer values.
-
-	"""
-
-	min_t = np.amin(np.concatenate((timeline1, timeline2)))
-	max_t = np.amax(np.concatenate((timeline1, timeline2)))
-	full_timeline = np.arange(min_t, max_t + 1)
-
-	index1 = [list(np.where(full_timeline == int(t))[0])[0] for t in timeline1]
-	index2 = [list(np.where(full_timeline == int(t))[0])[0] for t in timeline2]
-
-	return full_timeline, index1, index2
+def timeline_matching(timeline1, timeline2):
+    """
+    Match two timelines and create a unified timeline with corresponding indices.
+
+    Parameters
+    ----------
+    timeline1 : array-like
+            The first timeline to be matched.
+    timeline2 : array-like
+            The second timeline to be matched.
+
+    Returns
+    -------
+    tuple
+            A tuple containing:
+
+                    - full_timeline : numpy.ndarray
+                            The unified timeline spanning from the minimum to the maximum time point in the input timelines.
+                    - index1 : list of int
+                            The indices of `timeline1` in the `full_timeline`.
+                    - index2 : list of int
+                            The indices of `timeline2` in the `full_timeline`.
+
+    Examples
+    --------
+    >>> timeline1 = [1, 2, 5, 6]
+    >>> timeline2 = [2, 3, 4, 6]
+    >>> full_timeline, index1, index2 = timeline_matching(timeline1, timeline2)
+    >>> print(full_timeline)
+    [1 2 3 4 5 6]
+    >>> print(index1)
+    [0, 1, 4, 5]
+    >>> print(index2)
+    [1, 2, 3, 5]
+
+    Notes
+    -----
+    - The function combines the two timelines and generates a continuous range from the minimum to the maximum time point.
+    - It then finds the indices of the original timelines in this unified timeline.
+    - The function assumes that the input timelines consist of integer values.
+
+    """
+
+    min_t = np.amin(np.concatenate((timeline1, timeline2)))
+    max_t = np.amax(np.concatenate((timeline1, timeline2)))
+    full_timeline = np.arange(min_t, max_t + 1)
+
+    index1 = [list(np.where(full_timeline == int(t))[0])[0] for t in timeline1]
+    index2 = [list(np.where(full_timeline == int(t))[0])[0] for t in timeline2]
+
+    return full_timeline, index1, index2
 
 
 def rel_measure_at_position(pos):
 
-	pos = pos.replace('\\', '/')
-	pos = rf"{pos}"
-	assert os.path.exists(pos), f'Position {pos} is not a valid path.'
-	if not pos.endswith('/'):
-		pos += '/'
-	script_path = os.sep.join([abs_path, 'scripts', 'measure_relative.py'])
-	cmd = f'python "{script_path}" --pos "{pos}"'
-	subprocess.call(cmd, shell=True)
+    pos = pos.replace("\\", "/")
+    pos = rf"{pos}"
+    assert os.path.exists(pos), f"Position {pos} is not a valid path."
+    if not pos.endswith("/"):
+        pos += "/"
+    script_path = os.sep.join([abs_path, "scripts", "measure_relative.py"])
+    cmd = f'python "{script_path}" --pos "{pos}"'
+    subprocess.call(cmd, shell=True)
 
 
 # def mcf7_size_model(x,x0,x2):
@@ -520,265 +729,333 @@ def rel_measure_at_position(pos):
 # 					probs.append(group)
 # 	return probs
 
+
 def update_effector_table(df_relative, df_effector):
-	df_effector['group_neighborhood']=1
-	effectors = np.unique(df_relative['EFFECTOR_ID'].to_numpy())
-	for effector in effectors:
-		try:
-			# Set group_neighborhood to 0 where TRACK_ID matches effector
-			df_effector.loc[df_effector['TRACK_ID'] == effector, 'group_neighborhood'] = 0
-		except:
-			df_effector.loc[df_effector['ID'] == effector, 'group_neighborhood'] = 0
-	return df_effector
-
-def extract_neighborhoods_from_pickles(pos, populations=['targets','effectors']):
-
-	"""
-	Extract neighborhood protocols from pickle files located at a given position.
-
-	Parameters
-	----------
-	pos : str
-		The base directory path where the pickle files are located.
-
-	Returns
-	-------
-	list of dict
-		A list of dictionaries, each containing a neighborhood protocol. Each dictionary has the keys:
-			
-			- 'reference' : str
-				The reference population ('targets' or 'effectors').
-			- 'neighbor' : str
-				The neighbor population.
-			- 'type' : str
-				The type of neighborhood ('circle' or 'contact').
-			- 'distance' : float
-				The distance parameter for the neighborhood.
-			- 'description' : str
-				The original neighborhood string.
-
-	Notes
-	-----
-	- The function checks for the existence of pickle files containing target and effector trajectory data.
-	- If the files exist, it loads the data and extracts columns that start with 'neighborhood'.
-	- The neighborhood settings are extracted using the `extract_neighborhood_settings` function.
-	- The function assumes the presence of subdirectories 'output/tables' under the provided `pos`.
-
-	Examples
-	--------
-	>>> protocols = extract_neighborhoods_from_pickles('/path/to/data')
-	>>> for protocol in protocols:
-	>>>		print(protocol)
-	{'reference': 'targets', 'neighbor': 'targets', 'type': 'contact', 'distance': 5.0, 'description': 'neighborhood_self_contact_5_px'}
-	
-	"""
-
-	neighborhood_protocols = []
-
-	for pop in populations:
-		tab_pop = pos + os.sep.join(['output', 'tables', f'trajectories_{pop}.pkl'])
-		if os.path.exists(tab_pop):
-			df_pop = np.load(tab_pop, allow_pickle=True)
-			for column in list(df_pop.columns):
-				if column.startswith('neighborhood'):
-					neigh_protocol = extract_neighborhood_settings(column, population=pop)
-					neighborhood_protocols.append(neigh_protocol)
-
-	# tab_tc = pos + os.sep.join(['output', 'tables', 'trajectories_targets.pkl'])
-	# if os.path.exists(tab_tc):
-	# 	df_targets = np.load(tab_tc, allow_pickle=True)
-	# else:
-	# 	df_targets = None
-	# if os.path.exists(tab_tc.replace('targets','effectors')):
-	# 	df_effectors = np.load(tab_tc.replace('targets','effectors'), allow_pickle=True)
-	# else:
-	# 	df_effectors = None
-
-	# neighborhood_protocols=[]
-
-	# if df_targets is not None:
-	# 	for column in list(df_targets.columns):
-	# 		if column.startswith('neighborhood'):
-	# 			neigh_protocol = extract_neighborhood_settings(column, population='targets')
-	# 			neighborhood_protocols.append(neigh_protocol)
-
-	# if df_effectors is not None:
-	# 	for column in list(df_effectors.columns):
-	# 		if column.startswith('neighborhood'):
-	# 			neigh_protocol = extract_neighborhood_settings(column, population='effectors')
-	# 			neighborhood_protocols.append(neigh_protocol)
-
-	return neighborhood_protocols
-
-def extract_neighborhood_settings(neigh_string, population='targets'):
-	
-	"""
-	Extract neighborhood settings from a given string.
-
-	Parameters
-	----------
-	neigh_string : str
-		The string describing the neighborhood settings. Must start with 'neighborhood'.
-	population : str, optional
-		The population type ('targets' by default). Can be either 'targets' or 'effectors'.
-
-	Returns
-	-------
-	dict
-		A dictionary containing the neighborhood protocol with keys:
-		
-			- 'reference' : str
-				The reference population.
-			- 'neighbor' : str
-				The neighbor population.
-			- 'type' : str
-				The type of neighborhood ('circle' or 'contact').
-			- 'distance' : float
-				The distance parameter for the neighborhood.
-			- 'description' : str
-				The original neighborhood string.
-
-	Raises
-	------
-	AssertionError
-		If the `neigh_string` does not start with 'neighborhood'.
-
-	Notes
-	-----
-	- The function determines the neighbor population based on the given population.
-	- The neighborhood type and distance are extracted from the `neigh_string`.
-	- The description field in the returned dictionary contains the original neighborhood string.
-
-	Examples
-	--------
-	>>> extract_neighborhood_settings('neighborhood_self_contact_5_px', 'targets')
-	{'reference': 'targets', 'neighbor': 'targets', 'type': 'contact', 'distance': 5.0, 'description': 'neighborhood_self_contact_5_px'}
-	
-	"""
-
-	assert neigh_string.startswith('neighborhood')
-	print(f"{neigh_string=}")
-
-	if '_(' in neigh_string and ')_' in neigh_string:
-		# determine neigh pop from string
-		neighbor_population = neigh_string.split('_(')[-1].split(')_')[0].split('-')[-1]
-		print(f'{neighbor_population=}')
-	else:
-		# old method
-		if population=='targets':
-			neighbor_population = 'effectors'
-		elif population=='effectors':
-			neighbor_population = 'targets'
-
-	if 'self' in neigh_string:
-		
-		if 'circle' in neigh_string:
-			
-			distance = float(neigh_string.split('circle_')[1].replace('_px',''))
-			neigh_protocol = {'reference': population,'neighbor': population,'type':'circle','distance':distance,'description': neigh_string}
-		elif 'contact' in neigh_string:
-			distance=float(neigh_string.split('contact_')[1].replace('_px',''))
-			neigh_protocol = {'reference': population,'neighbor': population,'type':'contact','distance':distance,'description': neigh_string}
-	else:
-
-		if 'circle' in neigh_string:
-
-			distance=float(neigh_string.split('circle_')[1].replace('_px',''))
-			neigh_protocol = {'reference': population,'neighbor': neighbor_population,'type':'circle','distance':distance,'description': neigh_string}
-		elif 'contact' in neigh_string:
-			
-			distance=float(neigh_string.split('contact_')[1].replace('_px',''))
-			neigh_protocol = {'reference': population,'neighbor': neighbor_population,'type':'contact','distance':distance,'description': neigh_string}
-
-	return neigh_protocol
+    df_effector["group_neighborhood"] = 1
+    effectors = np.unique(df_relative["EFFECTOR_ID"].to_numpy())
+    for effector in effectors:
+        try:
+            # Set group_neighborhood to 0 where TRACK_ID matches effector
+            df_effector.loc[
+                df_effector["TRACK_ID"] == effector, "group_neighborhood"
+            ] = 0
+        except:
+            df_effector.loc[df_effector["ID"] == effector, "group_neighborhood"] = 0
+    return df_effector
+
+
+def extract_neighborhoods_from_pickles(pos, populations=["targets", "effectors"]):
+    """
+    Extract neighborhood protocols from pickle files located at a given position.
+
+    Parameters
+    ----------
+    pos : str
+            The base directory path where the pickle files are located.
+
+    Returns
+    -------
+    list of dict
+            A list of dictionaries, each containing a neighborhood protocol. Each dictionary has the keys:
+
+                    - 'reference' : str
+                            The reference population ('targets' or 'effectors').
+                    - 'neighbor' : str
+                            The neighbor population.
+                    - 'type' : str
+                            The type of neighborhood ('circle' or 'contact').
+                    - 'distance' : float
+                            The distance parameter for the neighborhood.
+                    - 'description' : str
+                            The original neighborhood string.
+
+    Notes
+    -----
+    - The function checks for the existence of pickle files containing target and effector trajectory data.
+    - If the files exist, it loads the data and extracts columns that start with 'neighborhood'.
+    - The neighborhood settings are extracted using the `extract_neighborhood_settings` function.
+    - The function assumes the presence of subdirectories 'output/tables' under the provided `pos`.
+
+    Examples
+    --------
+    >>> protocols = extract_neighborhoods_from_pickles('/path/to/data')
+    >>> for protocol in protocols:
+    >>>		print(protocol)
+    {'reference': 'targets', 'neighbor': 'targets', 'type': 'contact', 'distance': 5.0, 'description': 'neighborhood_self_contact_5_px'}
+
+    """
+
+    neighborhood_protocols = []
+
+    for pop in populations:
+        tab_pop = pos + os.sep.join(["output", "tables", f"trajectories_{pop}.pkl"])
+        if os.path.exists(tab_pop):
+            df_pop = np.load(tab_pop, allow_pickle=True)
+            for column in list(df_pop.columns):
+                if column.startswith("neighborhood"):
+                    neigh_protocol = extract_neighborhood_settings(
+                        column, population=pop
+                    )
+                    neighborhood_protocols.append(neigh_protocol)
+
+    # tab_tc = pos + os.sep.join(['output', 'tables', 'trajectories_targets.pkl'])
+    # if os.path.exists(tab_tc):
+    # 	df_targets = np.load(tab_tc, allow_pickle=True)
+    # else:
+    # 	df_targets = None
+    # if os.path.exists(tab_tc.replace('targets','effectors')):
+    # 	df_effectors = np.load(tab_tc.replace('targets','effectors'), allow_pickle=True)
+    # else:
+    # 	df_effectors = None
+
+    # neighborhood_protocols=[]
+
+    # if df_targets is not None:
+    # 	for column in list(df_targets.columns):
+    # 		if column.startswith('neighborhood'):
+    # 			neigh_protocol = extract_neighborhood_settings(column, population='targets')
+    # 			neighborhood_protocols.append(neigh_protocol)
+
+    # if df_effectors is not None:
+    # 	for column in list(df_effectors.columns):
+    # 		if column.startswith('neighborhood'):
+    # 			neigh_protocol = extract_neighborhood_settings(column, population='effectors')
+    # 			neighborhood_protocols.append(neigh_protocol)
+
+    return neighborhood_protocols
+
+
+def extract_neighborhood_settings(neigh_string, population="targets"):
+    """
+    Extract neighborhood settings from a given string.
+
+    Parameters
+    ----------
+    neigh_string : str
+            The string describing the neighborhood settings. Must start with 'neighborhood'.
+    population : str, optional
+            The population type ('targets' by default). Can be either 'targets' or 'effectors'.
+
+    Returns
+    -------
+    dict
+            A dictionary containing the neighborhood protocol with keys:
+
+                    - 'reference' : str
+                            The reference population.
+                    - 'neighbor' : str
+                            The neighbor population.
+                    - 'type' : str
+                            The type of neighborhood ('circle' or 'contact').
+                    - 'distance' : float
+                            The distance parameter for the neighborhood.
+                    - 'description' : str
+                            The original neighborhood string.
+
+    Raises
+    ------
+    AssertionError
+            If the `neigh_string` does not start with 'neighborhood'.
+
+    Notes
+    -----
+    - The function determines the neighbor population based on the given population.
+    - The neighborhood type and distance are extracted from the `neigh_string`.
+    - The description field in the returned dictionary contains the original neighborhood string.
+
+    Examples
+    --------
+    >>> extract_neighborhood_settings('neighborhood_self_contact_5_px', 'targets')
+    {'reference': 'targets', 'neighbor': 'targets', 'type': 'contact', 'distance': 5.0, 'description': 'neighborhood_self_contact_5_px'}
+
+    """
+
+    assert neigh_string.startswith("neighborhood")
+    print(f"{neigh_string=}")
+
+    if "_(" in neigh_string and ")_" in neigh_string:
+        # determine neigh pop from string
+        neighbor_population = neigh_string.split("_(")[-1].split(")_")[0].split("-")[-1]
+        print(f"{neighbor_population=}")
+    else:
+        # old method
+        if population == "targets":
+            neighbor_population = "effectors"
+        elif population == "effectors":
+            neighbor_population = "targets"
+
+    if "self" in neigh_string:
+
+        if "circle" in neigh_string:
+
+            distance = float(neigh_string.split("circle_")[1].replace("_px", ""))
+            neigh_protocol = {
+                "reference": population,
+                "neighbor": population,
+                "type": "circle",
+                "distance": distance,
+                "description": neigh_string,
+            }
+        elif "contact" in neigh_string:
+            distance = float(neigh_string.split("contact_")[1].replace("_px", ""))
+            neigh_protocol = {
+                "reference": population,
+                "neighbor": population,
+                "type": "contact",
+                "distance": distance,
+                "description": neigh_string,
+            }
+    else:
+
+        if "circle" in neigh_string:
+
+            distance = float(neigh_string.split("circle_")[1].replace("_px", ""))
+            neigh_protocol = {
+                "reference": population,
+                "neighbor": neighbor_population,
+                "type": "circle",
+                "distance": distance,
+                "description": neigh_string,
+            }
+        elif "contact" in neigh_string:
+
+            distance = float(neigh_string.split("contact_")[1].replace("_px", ""))
+            neigh_protocol = {
+                "reference": population,
+                "neighbor": neighbor_population,
+                "type": "contact",
+                "distance": distance,
+                "description": neigh_string,
+            }
+
+    return neigh_protocol
 
 
 def expand_pair_table(data):
-
-	"""
-	Expands a pair table by merging reference and neighbor trajectory data from CSV files based on the specified 
-	reference and neighbor populations, and their associated positions and frames.
-
-	Parameters
-	----------
-	data : pandas.DataFrame
-		DataFrame containing the pair table
-
-	Returns
-	-------
-	pandas.DataFrame
-		Expanded DataFrame that includes merged reference and neighbor data, sorted by position, reference population, 
-		neighbor population, and frame. Rows without values in `REFERENCE_ID`, `NEIGHBOR_ID`, `reference_population`, 
-		or `neighbor_population` are dropped.
-
-	Notes
-	-----
-	- For each unique pair of `reference_population` and `neighbor_population`, the function identifies corresponding 
-	  trajectories CSV files based on the position identifier.
-	- The function reads the trajectories CSV files, prefixes columns with `reference_` or `neighbor_` to avoid 
-	  conflicts, and merges data from reference and neighbor tables based on `TRACK_ID` or `ID`, and `FRAME`.
-	- Merges are performed in an outer join manner to retain all rows, regardless of missing values in the target files.
-	- The final DataFrame is sorted and cleaned to ensure only valid pairings are included.
-
-	Example
-	-------
-	>>> expanded_df = expand_pair_table(pair_table)
-	>>> expanded_df.head()
-
-	Raises
-	------
-	AssertionError
-		If `reference_population` or `neighbor_population` is not found in the columns of `data`.
-	
-	"""
-
-	assert 'reference_population' in list(data.columns),"Please provide a valid pair table..."
-	assert 'neighbor_population' in list(data.columns),"Please provide a valid pair table..."
-
-	data.__dict__.update(data.astype({'reference_population': str, 'neighbor_population': str}).__dict__)
-
-	expanded_table = []
-	
-	for neigh, group in data.groupby(['reference_population','neighbor_population']):
-		
-		ref_pop = neigh[0]; neigh_pop = neigh[1];
-
-		for pos,pos_group in group.groupby('position'):
-			
-			ref_tab = os.sep.join([pos,'output','tables',f'trajectories_{ref_pop}.csv'])
-			neigh_tab = os.sep.join([pos,'output','tables',f'trajectories_{neigh_pop}.csv'])
-			
-			if os.path.exists(ref_tab):
-				df_ref = pd.read_csv(ref_tab)
-				if 'TRACK_ID' in df_ref.columns:
-					if not np.all(df_ref['TRACK_ID'].isnull()):
-						ref_merge_cols = ['TRACK_ID','FRAME']
-					else:
-						ref_merge_cols = ['ID','FRAME']
-				else:
-					ref_merge_cols = ['ID','FRAME']
-
-			if os.path.exists(neigh_tab):
-				df_neigh = pd.read_csv(neigh_tab)
-				if 'TRACK_ID' in df_neigh.columns:
-					if not np.all(df_neigh['TRACK_ID'].isnull()):
-						neigh_merge_cols = ['TRACK_ID','FRAME']
-					else:
-						neigh_merge_cols = ['ID','FRAME']
-				else:
-					neigh_merge_cols = ['ID','FRAME']
-
-			df_ref = df_ref.add_prefix('reference_',axis=1)
-			df_neigh = df_neigh.add_prefix('neighbor_',axis=1)
-			ref_merge_cols = ['reference_'+c for c in ref_merge_cols]
-			neigh_merge_cols = ['neighbor_'+c for c in neigh_merge_cols]
-
-			merge_ref = pos_group.merge(df_ref, how='outer', left_on=['REFERENCE_ID','FRAME'], right_on=ref_merge_cols, suffixes=('', '_reference'))
-			merge_neigh = merge_ref.merge(df_neigh, how='outer', left_on=['NEIGHBOR_ID','FRAME'], right_on=neigh_merge_cols, suffixes=('_reference', '_neighbor'))
-			expanded_table.append(merge_neigh)
-
-	df_expanded = pd.concat(expanded_table, axis=0, ignore_index = True)
-	df_expanded = df_expanded.sort_values(by=['position', 'reference_population','neighbor_population','REFERENCE_ID','NEIGHBOR_ID','FRAME'])
-	df_expanded = df_expanded.dropna(axis=0, subset=['REFERENCE_ID','NEIGHBOR_ID','reference_population','neighbor_population'])
-
-	return df_expanded
+    """
+    Expands a pair table by merging reference and neighbor trajectory data from CSV files based on the specified
+    reference and neighbor populations, and their associated positions and frames.
+
+    Parameters
+    ----------
+    data : pandas.DataFrame
+            DataFrame containing the pair table
+
+    Returns
+    -------
+    pandas.DataFrame
+            Expanded DataFrame that includes merged reference and neighbor data, sorted by position, reference population,
+            neighbor population, and frame. Rows without values in `REFERENCE_ID`, `NEIGHBOR_ID`, `reference_population`,
+            or `neighbor_population` are dropped.
+
+    Notes
+    -----
+    - For each unique pair of `reference_population` and `neighbor_population`, the function identifies corresponding
+      trajectories CSV files based on the position identifier.
+    - The function reads the trajectories CSV files, prefixes columns with `reference_` or `neighbor_` to avoid
+      conflicts, and merges data from reference and neighbor tables based on `TRACK_ID` or `ID`, and `FRAME`.
+    - Merges are performed in an outer join manner to retain all rows, regardless of missing values in the target files.
+    - The final DataFrame is sorted and cleaned to ensure only valid pairings are included.
+
+    Example
+    -------
+    >>> expanded_df = expand_pair_table(pair_table)
+    >>> expanded_df.head()
+
+    Raises
+    ------
+    AssertionError
+            If `reference_population` or `neighbor_population` is not found in the columns of `data`.
+
+    """
+
+    assert "reference_population" in list(
+        data.columns
+    ), "Please provide a valid pair table..."
+    assert "neighbor_population" in list(
+        data.columns
+    ), "Please provide a valid pair table..."
+
+    data.__dict__.update(
+        data.astype({"reference_population": str, "neighbor_population": str}).__dict__
+    )
+
+    expanded_table = []
+
+    for neigh, group in data.groupby(["reference_population", "neighbor_population"]):
+
+        ref_pop = neigh[0]
+        neigh_pop = neigh[1]
+
+        for pos, pos_group in group.groupby("position"):
+
+            ref_tab = os.sep.join(
+                [pos, "output", "tables", f"trajectories_{ref_pop}.csv"]
+            )
+            neigh_tab = os.sep.join(
+                [pos, "output", "tables", f"trajectories_{neigh_pop}.csv"]
+            )
+
+            if os.path.exists(ref_tab):
+                df_ref = pd.read_csv(ref_tab)
+                if "TRACK_ID" in df_ref.columns:
+                    if not np.all(df_ref["TRACK_ID"].isnull()):
+                        ref_merge_cols = ["TRACK_ID", "FRAME"]
+                    else:
+                        ref_merge_cols = ["ID", "FRAME"]
+                else:
+                    ref_merge_cols = ["ID", "FRAME"]
+
+            if os.path.exists(neigh_tab):
+                df_neigh = pd.read_csv(neigh_tab)
+                if "TRACK_ID" in df_neigh.columns:
+                    if not np.all(df_neigh["TRACK_ID"].isnull()):
+                        neigh_merge_cols = ["TRACK_ID", "FRAME"]
+                    else:
+                        neigh_merge_cols = ["ID", "FRAME"]
+                else:
+                    neigh_merge_cols = ["ID", "FRAME"]
+
+            df_ref = df_ref.add_prefix("reference_", axis=1)
+            df_neigh = df_neigh.add_prefix("neighbor_", axis=1)
+            ref_merge_cols = ["reference_" + c for c in ref_merge_cols]
+            neigh_merge_cols = ["neighbor_" + c for c in neigh_merge_cols]
+
+            merge_ref = pos_group.merge(
+                df_ref,
+                how="outer",
+                left_on=["REFERENCE_ID", "FRAME"],
+                right_on=ref_merge_cols,
+                suffixes=("", "_reference"),
+            )
+            merge_neigh = merge_ref.merge(
+                df_neigh,
+                how="outer",
+                left_on=["NEIGHBOR_ID", "FRAME"],
+                right_on=neigh_merge_cols,
+                suffixes=("_reference", "_neighbor"),
+            )
+            expanded_table.append(merge_neigh)
+
+    df_expanded = pd.concat(expanded_table, axis=0, ignore_index=True)
+    df_expanded = df_expanded.sort_values(
+        by=[
+            "position",
+            "reference_population",
+            "neighbor_population",
+            "REFERENCE_ID",
+            "NEIGHBOR_ID",
+            "FRAME",
+        ]
+    )
+    df_expanded = df_expanded.dropna(
+        axis=0,
+        subset=[
+            "REFERENCE_ID",
+            "NEIGHBOR_ID",
+            "reference_population",
+            "neighbor_population",
+        ],
+    )
+
+    return df_expanded