biopipen 0.34.0__py3-none-any.whl → 0.34.2__py3-none-any.whl
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- biopipen/__init__.py +1 -1
- biopipen/ns/scrna.py +276 -30
- biopipen/ns/tcr.py +4 -4
- biopipen/scripts/scrna/MarkersFinder.R +190 -49
- biopipen/scripts/scrna/PseudoBulkDEG.R +592 -0
- biopipen/scripts/scrna/ScFGSEA.R +101 -28
- biopipen/scripts/scrna/SeuratClusterStats-features.R +4 -0
- biopipen/scripts/scrna/TopExpressingGenes.R +9 -7
- biopipen/scripts/tcr/ClonalStats.R +1 -1
- biopipen/scripts/tcr/ScRepCombiningExpression.R +2 -2
- {biopipen-0.34.0.dist-info → biopipen-0.34.2.dist-info}/METADATA +1 -1
- {biopipen-0.34.0.dist-info → biopipen-0.34.2.dist-info}/RECORD +14 -15
- biopipen/reports/scrna/ScFGSEA.svelte +0 -16
- biopipen/reports/scrna/TopExpressingGenes.svelte +0 -17
- {biopipen-0.34.0.dist-info → biopipen-0.34.2.dist-info}/WHEEL +0 -0
- {biopipen-0.34.0.dist-info → biopipen-0.34.2.dist-info}/entry_points.txt +0 -0
biopipen/scripts/scrna/ScFGSEA.R
CHANGED
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@@ -7,9 +7,9 @@ srtfile <- {{in.srtobj | r}} # nolint
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outdir <- {{out.outdir | r}} # nolint
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joboutdir <- {{job.outdir | r}} # nolint
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mutaters <- {{envs.mutaters | r}} # nolint
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group_by <- {{envs.group_by | default: envs["group-by"] | default: None | r}} # nolint
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ident_1 <- {{envs.ident_1 | default: envs["ident-1"] | default: None | r}} # nolint
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ident_2 <- {{envs.ident_2 | default: envs["ident-2"] | default: None | r}} # nolint
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each <- {{envs.each | r}} # nolint
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subset <- {{envs.subset | r}} # nolint
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gmtfile <- {{envs.gmtfile | r}} # nolint
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@@ -18,6 +18,8 @@ top <- {{envs.top | r}} # nolint
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minsize <- {{envs.minSize | default: envs.minsize | r}} # nolint
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maxsize <- {{envs.maxSize | default: envs.maxsize | r}} # nolint
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eps <- {{envs.eps | r}} # nolint
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alleach_plots_defaults <- {{envs.alleach_plots_defaults | r}} # nolint
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alleach_plots <- {{envs.alleach_plots | r}} #
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ncores <- {{envs.ncores | r}} # nolint
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rest <- {{envs.rest | r: todot="-"}} # nolint
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cases <- {{envs.cases | r: todot="-"}} # nolint
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@@ -25,6 +27,10 @@ cases <- {{envs.cases | r: todot="-"}} # nolint
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log <- get_logger()
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reporter <- get_reporter()
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alleach_plots <- lapply(alleach_plots, function(x) {
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list_update(alleach_plots_defaults, x)
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})
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log$info("Reading Seurat object ...")
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srtobj <- read_obj(srtfile)
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if (!"Identity" %in% colnames(srtobj@meta.data)) {
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@@ -37,9 +43,9 @@ if (!is.null(mutaters) && length(mutaters) > 0) {
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}
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defaults <- list(
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-
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group_by = group_by,
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ident_1 = ident_1,
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ident_2 = ident_2,
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each = each,
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subset = subset,
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gmtfile = gmtfile,
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@@ -48,6 +54,8 @@ defaults <- list(
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minsize = minsize,
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maxsize = maxsize,
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eps = eps,
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alleach_plots_defaults = alleach_plots_defaults,
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alleach_plots = alleach_plots,
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ncores = ncores,
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rest = rest
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)
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@@ -55,9 +63,13 @@ defaults <- list(
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expand_each <- function(name, case) {
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outcases <- list()
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case$
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case$group_by <- case$group_by %||% "Identity"
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if (is.null(case$each) || is.na(case$each) || nchar(case$each) == 0 || isFALSE(each)) {
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if (length(case$alleach_plots) > 0) {
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stop("Cannot perform `alleach_plots` without `each` defined.")
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}
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outcases[[name]] <- case
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} else {
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eachs <- if (!is.null(case$subset)) {
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@@ -77,10 +89,13 @@ expand_each <- function(name, case) {
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newname <- paste0(case$each, "::", each)
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newcase <- case
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newcase$original_case <- name
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newcase$original_case <- paste0(name, " (all ", case$each,")")
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newcase$each_name <- case$each
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newcase$each <- each
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newcase$alleach_plots_defaults <- NULL
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newcase$alleach_plots <- NULL
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if (!is.null(case$subset)) {
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newcase$subset <- paste0(case$subset, " & ", bQuote(case$each), " == '", each, "'")
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} else {
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outcases[[newname]] <- newcase
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}
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if (length(case$alleach_plots) > 0) {
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newcase <- case
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newcase$gseas <- list()
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newcase$alleach_plots <- lapply(
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newcase$alleach_plots,
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function(x) { list_update(newcase$alleach_plots_defaults, x) }
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)
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outcases[[paste0(name, " (all ", case$each,")")]] <- newcase
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}
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}
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outcases
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}
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@@ -108,21 +135,59 @@ ensure_sobj <- function(expr, allow_empty) {
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})
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}
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-
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do_case <- function(name) {
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log$info("- Processing case: {name} ...")
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case <- cases[[name]]
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info <- case_info(name, outdir, create = TRUE)
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if (!is.null(case$gseas)) {
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each_levels <- names(case$gseas)
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gseas <- do_call(rbind, lapply(each_levels, function(x) {
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gsea_df <- case$gseas[[x]]
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if (nrow(gsea_df) > 0) {
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gsea_df[[case$each]] <- x
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} else {
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gsea_df[[case$each]] <- character(0) # Empty case
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}
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gsea_df
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}))
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gseas[[case$each]] <- factor(gseas[[case$each]], levels = each_levels)
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for (plotname in names(case$alleach_plots)) {
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plotargs <- case$alleach_plots[[plotname]]
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plotargs <- extract_vars(plotargs, "devpars")
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plotargs$gsea_results <- gseas
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plotargs$group_by <- case$each
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if (plotargs$plot_type == "heatmap") {
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plotargs$show_row_names <- plotargs$show_row_names %||% TRUE
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plotargs$show_column_names <- plotargs$show_column_names %||% TRUE
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}
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p <- do_call(VizGSEA, plotargs)
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outprefix <- file.path(info$prefix, paste0("all.", slugify(plotname)))
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save_plot(p, outprefix, devpars, formats = "png")
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reporter$add2(
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list(kind = "descr", content = paste0("Pathways for all ", case$each, ".")),
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list(kind = "image", src = paste0(outprefix, ".png")),
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hs = c(info$section, info$name),
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hs2 = plotname
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)
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}
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return(invisible(NULL))
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}
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allow_empty = !is.null(case$each)
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# prepare expression matrix
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log$info(" Preparing expression matrix...")
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sobj <- ensure_sobj({ srtobj %>% filter(!is.na(!!sym(case$
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sobj <- ensure_sobj({ srtobj %>% filter(!is.na(!!sym(case$group_by))) }, allow_empty)
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if (is.null(sobj)) {
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reporter$add2(
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list(
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kind = "error",
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content = paste0("No cells with non-NA `", case$
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content = paste0("No cells with non-NA `", case$group_by, "` in the Seurat object.")
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),
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hs = c(info$section, info$name)
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)
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reporter$add2(
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list(
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kind = "error",
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content = paste0("No cells with non-NA `", case$
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content = paste0("No cells with non-NA `", case$group_by, "` in the Seurat object.")
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),
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hs = c(info$section, info$name)
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)
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return(NULL)
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}
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}
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if (!is.null(case$
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sobj <- ensure_sobj({ sobj %>% filter(!!sym(case$
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if (!is.null(case$ident_2)) {
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sobj <- ensure_sobj({ sobj %>% filter(!!sym(case$group_by) %in% c(case$ident_1, case$ident_2)) }, allow_empty)
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if (is.null(sobj)) {
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reporter$add2(
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list(
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kind = "error",
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content = paste0("No cells with non-NA `", case$
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content = paste0("No cells with non-NA `", case$group_by, "` in the Seurat object.")
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),
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hs = c(info$section, info$name)
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)
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}
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}
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allclasses <- sobj@meta.data[, case$
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if (is.null(case$
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case$
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allclasses[allclasses != case$
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allclasses <- sobj@meta.data[, case$group_by, drop = TRUE]
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if (is.null(case$ident_2)) {
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case$ident_2 <- "Other"
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allclasses[allclasses != case$ident_1] <- "Other"
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}
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exprs <- GetAssayData(sobj, layer = "data")
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# get preranks
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log$info(" Getting preranks...")
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ranks <- RunGSEAPreRank(exprs, allclasses, case$
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ranks <- RunGSEAPreRank(exprs, allclasses, case$ident_1, case$ident_2, case$method)
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write.table(
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ranks,
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file.path(info$prefix, "fgsea.rank"),
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row.names =
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as.data.frame(ranks),
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file.path(info$prefix, "fgsea.rank.txt"),
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row.names = TRUE,
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col.names = TRUE,
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sep = "\t",
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quote = FALSE
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quote = FALSE
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)
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aspect.ratio <- sqrt(case$top) / sqrt(10)
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p_summary <- VizGSEA(
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result,
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plot_type = "summary",
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top_term = case$top
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top_term = case$top,
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aspect.ratio = aspect.ratio
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)
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save_plot(
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p_summary,
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file.path(info$prefix, "summary"),
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devpars = list(res = 100, height = attr(p_summary, "height") * 100, width = attr(p_summary, "width") * 100),
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devpars = list(res = 100, height = attr(p_summary, "height") * 100 / 1.5, width = attr(p_summary, "width") * 100),
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formats = "png"
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)
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reporter$add2(
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list(
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name = "Table",
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name = paste0("Table (", case$ident_1, " vs ", case$ident_2, ")"),
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contents = list(
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list(kind = "descr", content = paste0(
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"Showing top 50 pathways by padj in descending order. ",
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"Use 'Download the entire data' button to download all pathways."
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)),
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list(kind = "table", src = file.path(info$prefix, "fgsea"), data = list(nrows = 50))
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list(kind = "table", src = file.path(info$prefix, "fgsea.tsv"), data = list(nrows = 50))
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),
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list(
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hs = c(info$section, info$name),
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ui = "tabs"
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)
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if (!is.null(case$original_case) && !is.null(cases[[case$original_case]])) {
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cases[[case$original_case]]$gseas[[case$each]] <<- result
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}
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invisible()
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}
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sapply(
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sapply(names(cases), function(name) do_case(name))
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reporter$save(joboutdir)
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@@ -9,7 +9,7 @@ outdir <- {{out.outdir | r}}
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joboutdir <- {{job.outdir | r}}
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mutaters <- {{ envs.mutaters | r }}
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ident <- {{ envs.ident | r }}
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group_by <- {{ envs.group_by | default: envs["group-by"] | default: None | r }} # nolint
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each <- {{ envs.each | r }}
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dbs <- {{ envs.dbs | r }}
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n <- {{ envs.n | r }}
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@@ -41,7 +41,7 @@ enrich_plots <- lapply(enrich_plots, function(x) {
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})
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defaults <- list(
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ident = ident,
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-
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group_by = group_by,
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each = each,
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{%- macro report_job(job, h=1) -%}
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10
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{{ job | render_job: h=h }}
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11
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{%- endmacro -%}
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12
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-
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13
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{%- macro head_job(job) -%}
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14
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<h1>{{job.in.srtobj | stem0 | escape}}</h1>
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15
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{%- endmacro -%}
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16
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-
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17
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{{ report_jobs(jobs, head_job, report_job) }}
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File without changes
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File without changes
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