@bgicli/bgicli 2.1.1 → 2.2.1
This diff represents the content of publicly available package versions that have been released to one of the supported registries. The information contained in this diff is provided for informational purposes only and reflects changes between package versions as they appear in their respective public registries.
- package/README.md +152 -74
- package/data/skills/aav-vector-design-agent/SKILL.md +198 -0
- package/data/skills/adaptyv/SKILL.md +112 -0
- package/data/skills/adhd-daily-planner/SKILL.md +271 -0
- package/data/skills/aeon/SKILL.md +372 -0
- package/data/skills/agent-browser/SKILL.md +159 -0
- package/data/skills/agentd-drug-discovery/SKILL.md +52 -0
- package/data/skills/ai-analyzer/SKILL.md +218 -0
- package/data/skills/alphafold/SKILL.md +183 -0
- package/data/skills/alphafold-database/SKILL.md +500 -0
- package/data/skills/anndata/SKILL.md +394 -0
- package/data/skills/antibody-design-agent/SKILL.md +64 -0
- package/data/skills/arboreto/SKILL.md +237 -0
- package/data/skills/armored-cart-design-agent/SKILL.md +225 -0
- package/data/skills/arxiv-search/SKILL.md +224 -0
- package/data/skills/autonomous-oncology-agent/SKILL.md +77 -0
- package/data/skills/bayesian-optimizer/SKILL.md +60 -0
- package/data/skills/benchling-integration/SKILL.md +473 -0
- package/data/skills/bgpt-paper-search/SKILL.md +81 -0
- package/data/skills/bindcraft/SKILL.md +198 -0
- package/data/skills/binder-design/SKILL.md +182 -0
- package/data/skills/binding-characterization/SKILL.md +234 -0
- package/data/skills/bindingdb-database/SKILL.md +332 -0
- package/data/skills/bio-admet-prediction/SKILL.md +224 -0
- package/data/skills/bio-alignment-files-bam-statistics/SKILL.md +340 -0
- package/data/skills/bio-alignment-filtering/SKILL.md +322 -0
- package/data/skills/bio-alignment-indexing/SKILL.md +249 -0
- package/data/skills/bio-alignment-io/SKILL.md +301 -0
- package/data/skills/bio-alignment-msa-parsing/SKILL.md +366 -0
- package/data/skills/bio-alignment-msa-statistics/SKILL.md +375 -0
- package/data/skills/bio-alignment-pairwise/SKILL.md +277 -0
- package/data/skills/bio-alignment-sorting/SKILL.md +296 -0
- package/data/skills/bio-alignment-validation/SKILL.md +374 -0
- package/data/skills/bio-atac-seq-atac-peak-calling/SKILL.md +221 -0
- package/data/skills/bio-atac-seq-atac-qc/SKILL.md +292 -0
- package/data/skills/bio-atac-seq-differential-accessibility/SKILL.md +268 -0
- package/data/skills/bio-atac-seq-footprinting/SKILL.md +256 -0
- package/data/skills/bio-atac-seq-motif-deviation/SKILL.md +319 -0
- package/data/skills/bio-atac-seq-nucleosome-positioning/SKILL.md +321 -0
- package/data/skills/bio-basecalling/SKILL.md +368 -0
- package/data/skills/bio-batch-downloads/SKILL.md +384 -0
- package/data/skills/bio-batch-processing/SKILL.md +303 -0
- package/data/skills/bio-bedgraph-handling/SKILL.md +336 -0
- package/data/skills/bio-blast-searches/SKILL.md +354 -0
- package/data/skills/bio-causal-genomics-colocalization-analysis/SKILL.md +264 -0
- package/data/skills/bio-causal-genomics-fine-mapping/SKILL.md +267 -0
- package/data/skills/bio-causal-genomics-mediation-analysis/SKILL.md +264 -0
- package/data/skills/bio-causal-genomics-mendelian-randomization/SKILL.md +221 -0
- package/data/skills/bio-causal-genomics-pleiotropy-detection/SKILL.md +292 -0
- package/data/skills/bio-cfdna-preprocessing/SKILL.md +200 -0
- package/data/skills/bio-chipseq-differential-binding/SKILL.md +262 -0
- package/data/skills/bio-chipseq-motif-analysis/SKILL.md +387 -0
- package/data/skills/bio-chipseq-peak-annotation/SKILL.md +239 -0
- package/data/skills/bio-chipseq-peak-calling/SKILL.md +277 -0
- package/data/skills/bio-chipseq-qc/SKILL.md +391 -0
- package/data/skills/bio-chipseq-super-enhancers/SKILL.md +288 -0
- package/data/skills/bio-chipseq-visualization/SKILL.md +289 -0
- package/data/skills/bio-clinical-databases-clinvar-lookup/SKILL.md +188 -0
- package/data/skills/bio-clinical-databases-dbsnp-queries/SKILL.md +171 -0
- package/data/skills/bio-clinical-databases-gnomad-frequencies/SKILL.md +205 -0
- package/data/skills/bio-clinical-databases-hla-typing/SKILL.md +248 -0
- package/data/skills/bio-clinical-databases-myvariant-queries/SKILL.md +174 -0
- package/data/skills/bio-clinical-databases-pharmacogenomics/SKILL.md +232 -0
- package/data/skills/bio-clinical-databases-polygenic-risk/SKILL.md +276 -0
- package/data/skills/bio-clinical-databases-somatic-signatures/SKILL.md +261 -0
- package/data/skills/bio-clinical-databases-tumor-mutational-burden/SKILL.md +301 -0
- package/data/skills/bio-clinical-databases-variant-prioritization/SKILL.md +225 -0
- package/data/skills/bio-clip-seq-binding-site-annotation/SKILL.md +66 -0
- package/data/skills/bio-clip-seq-clip-alignment/SKILL.md +70 -0
- package/data/skills/bio-clip-seq-clip-motif-analysis/SKILL.md +62 -0
- package/data/skills/bio-clip-seq-clip-peak-calling/SKILL.md +282 -0
- package/data/skills/bio-clip-seq-clip-preprocessing/SKILL.md +142 -0
- package/data/skills/bio-codon-usage/SKILL.md +353 -0
- package/data/skills/bio-comparative-genomics-ancestral-reconstruction/SKILL.md +312 -0
- package/data/skills/bio-comparative-genomics-hgt-detection/SKILL.md +341 -0
- package/data/skills/bio-comparative-genomics-ortholog-inference/SKILL.md +308 -0
- package/data/skills/bio-comparative-genomics-positive-selection/SKILL.md +354 -0
- package/data/skills/bio-comparative-genomics-synteny-analysis/SKILL.md +315 -0
- package/data/skills/bio-compressed-files/SKILL.md +263 -0
- package/data/skills/bio-consensus-sequences/SKILL.md +340 -0
- package/data/skills/bio-copy-number-cnv-annotation/SKILL.md +307 -0
- package/data/skills/bio-copy-number-cnv-visualization/SKILL.md +294 -0
- package/data/skills/bio-copy-number-cnvkit-analysis/SKILL.md +290 -0
- package/data/skills/bio-copy-number-gatk-cnv/SKILL.md +270 -0
- package/data/skills/bio-crispr-screens-base-editing-analysis/SKILL.md +110 -0
- package/data/skills/bio-crispr-screens-batch-correction/SKILL.md +316 -0
- package/data/skills/bio-crispr-screens-crispresso-editing/SKILL.md +205 -0
- package/data/skills/bio-crispr-screens-hit-calling/SKILL.md +264 -0
- package/data/skills/bio-crispr-screens-jacks-analysis/SKILL.md +313 -0
- package/data/skills/bio-crispr-screens-library-design/SKILL.md +417 -0
- package/data/skills/bio-crispr-screens-mageck-analysis/SKILL.md +222 -0
- package/data/skills/bio-crispr-screens-screen-qc/SKILL.md +243 -0
- package/data/skills/bio-ctdna-mutation-detection/SKILL.md +234 -0
- package/data/skills/bio-data-visualization-circos-plots/SKILL.md +405 -0
- package/data/skills/bio-data-visualization-color-palettes/SKILL.md +244 -0
- package/data/skills/bio-data-visualization-genome-browser-tracks/SKILL.md +328 -0
- package/data/skills/bio-data-visualization-genome-tracks/SKILL.md +249 -0
- package/data/skills/bio-data-visualization-ggplot2-fundamentals/SKILL.md +313 -0
- package/data/skills/bio-data-visualization-heatmaps-clustering/SKILL.md +227 -0
- package/data/skills/bio-data-visualization-interactive-visualization/SKILL.md +210 -0
- package/data/skills/bio-data-visualization-multipanel-figures/SKILL.md +274 -0
- package/data/skills/bio-data-visualization-specialized-omics-plots/SKILL.md +251 -0
- package/data/skills/bio-data-visualization-upset-plots/SKILL.md +228 -0
- package/data/skills/bio-data-visualization-volcano-customization/SKILL.md +233 -0
- package/data/skills/bio-de-deseq2-basics/SKILL.md +376 -0
- package/data/skills/bio-de-edger-basics/SKILL.md +418 -0
- package/data/skills/bio-de-results/SKILL.md +378 -0
- package/data/skills/bio-de-visualization/SKILL.md +408 -0
- package/data/skills/bio-differential-expression-batch-correction/SKILL.md +253 -0
- package/data/skills/bio-differential-expression-timeseries-de/SKILL.md +370 -0
- package/data/skills/bio-differential-splicing/SKILL.md +177 -0
- package/data/skills/bio-duplicate-handling/SKILL.md +292 -0
- package/data/skills/bio-entrez-fetch/SKILL.md +334 -0
- package/data/skills/bio-entrez-link/SKILL.md +325 -0
- package/data/skills/bio-entrez-search/SKILL.md +311 -0
- package/data/skills/bio-epidemiological-genomics-amr-surveillance/SKILL.md +233 -0
- package/data/skills/bio-epidemiological-genomics-pathogen-typing/SKILL.md +202 -0
- package/data/skills/bio-epidemiological-genomics-phylodynamics/SKILL.md +207 -0
- package/data/skills/bio-epidemiological-genomics-transmission-inference/SKILL.md +237 -0
- package/data/skills/bio-epidemiological-genomics-variant-surveillance/SKILL.md +237 -0
- package/data/skills/bio-epitranscriptomics-m6a-differential/SKILL.md +88 -0
- package/data/skills/bio-epitranscriptomics-m6a-peak-calling/SKILL.md +89 -0
- package/data/skills/bio-epitranscriptomics-m6anet-analysis/SKILL.md +101 -0
- package/data/skills/bio-epitranscriptomics-merip-preprocessing/SKILL.md +81 -0
- package/data/skills/bio-epitranscriptomics-modification-visualization/SKILL.md +98 -0
- package/data/skills/bio-experimental-design-batch-design/SKILL.md +110 -0
- package/data/skills/bio-experimental-design-multiple-testing/SKILL.md +98 -0
- package/data/skills/bio-experimental-design-power-analysis/SKILL.md +84 -0
- package/data/skills/bio-experimental-design-sample-size/SKILL.md +93 -0
- package/data/skills/bio-expression-matrix-counts-ingest/SKILL.md +220 -0
- package/data/skills/bio-expression-matrix-gene-id-mapping/SKILL.md +256 -0
- package/data/skills/bio-expression-matrix-metadata-joins/SKILL.md +271 -0
- package/data/skills/bio-expression-matrix-sparse-handling/SKILL.md +247 -0
- package/data/skills/bio-fastq-quality/SKILL.md +279 -0
- package/data/skills/bio-filter-sequences/SKILL.md +265 -0
- package/data/skills/bio-flow-cytometry-bead-normalization/SKILL.md +315 -0
- package/data/skills/bio-flow-cytometry-clustering-phenotyping/SKILL.md +237 -0
- package/data/skills/bio-flow-cytometry-compensation-transformation/SKILL.md +196 -0
- package/data/skills/bio-flow-cytometry-cytometry-qc/SKILL.md +382 -0
- package/data/skills/bio-flow-cytometry-differential-analysis/SKILL.md +217 -0
- package/data/skills/bio-flow-cytometry-doublet-detection/SKILL.md +288 -0
- package/data/skills/bio-flow-cytometry-fcs-handling/SKILL.md +221 -0
- package/data/skills/bio-flow-cytometry-gating-analysis/SKILL.md +193 -0
- package/data/skills/bio-format-conversion/SKILL.md +193 -0
- package/data/skills/bio-fragment-analysis/SKILL.md +214 -0
- package/data/skills/bio-gatk-variant-calling/SKILL.md +422 -0
- package/data/skills/bio-genome-assembly-assembly-polishing/SKILL.md +333 -0
- package/data/skills/bio-genome-assembly-assembly-qc/SKILL.md +344 -0
- package/data/skills/bio-genome-assembly-contamination-detection/SKILL.md +235 -0
- package/data/skills/bio-genome-assembly-hifi-assembly/SKILL.md +178 -0
- package/data/skills/bio-genome-assembly-long-read-assembly/SKILL.md +307 -0
- package/data/skills/bio-genome-assembly-metagenome-assembly/SKILL.md +227 -0
- package/data/skills/bio-genome-assembly-scaffolding/SKILL.md +204 -0
- package/data/skills/bio-genome-assembly-short-read-assembly/SKILL.md +319 -0
- package/data/skills/bio-genome-engineering-base-editing-design/SKILL.md +277 -0
- package/data/skills/bio-genome-engineering-grna-design/SKILL.md +221 -0
- package/data/skills/bio-genome-engineering-hdr-template-design/SKILL.md +264 -0
- package/data/skills/bio-genome-engineering-off-target-prediction/SKILL.md +232 -0
- package/data/skills/bio-genome-engineering-prime-editing-design/SKILL.md +275 -0
- package/data/skills/bio-genome-intervals-bed-file-basics/SKILL.md +357 -0
- package/data/skills/bio-genome-intervals-bigwig-tracks/SKILL.md +351 -0
- package/data/skills/bio-genome-intervals-coverage-analysis/SKILL.md +300 -0
- package/data/skills/bio-genome-intervals-gtf-gff-handling/SKILL.md +345 -0
- package/data/skills/bio-genome-intervals-interval-arithmetic/SKILL.md +485 -0
- package/data/skills/bio-genome-intervals-proximity-operations/SKILL.md +337 -0
- package/data/skills/bio-geo-data/SKILL.md +380 -0
- package/data/skills/bio-hi-c-analysis-compartment-analysis/SKILL.md +261 -0
- package/data/skills/bio-hi-c-analysis-contact-pairs/SKILL.md +278 -0
- package/data/skills/bio-hi-c-analysis-hic-data-io/SKILL.md +260 -0
- package/data/skills/bio-hi-c-analysis-hic-differential/SKILL.md +328 -0
- package/data/skills/bio-hi-c-analysis-hic-visualization/SKILL.md +297 -0
- package/data/skills/bio-hi-c-analysis-loop-calling/SKILL.md +284 -0
- package/data/skills/bio-hi-c-analysis-matrix-operations/SKILL.md +274 -0
- package/data/skills/bio-hi-c-analysis-tad-detection/SKILL.md +239 -0
- package/data/skills/bio-imaging-mass-cytometry-cell-segmentation/SKILL.md +241 -0
- package/data/skills/bio-imaging-mass-cytometry-data-preprocessing/SKILL.md +279 -0
- package/data/skills/bio-imaging-mass-cytometry-interactive-annotation/SKILL.md +304 -0
- package/data/skills/bio-imaging-mass-cytometry-phenotyping/SKILL.md +231 -0
- package/data/skills/bio-imaging-mass-cytometry-quality-metrics/SKILL.md +316 -0
- package/data/skills/bio-imaging-mass-cytometry-spatial-analysis/SKILL.md +246 -0
- package/data/skills/bio-immunoinformatics-epitope-prediction/SKILL.md +259 -0
- package/data/skills/bio-immunoinformatics-immunogenicity-scoring/SKILL.md +275 -0
- package/data/skills/bio-immunoinformatics-mhc-binding-prediction/SKILL.md +260 -0
- package/data/skills/bio-immunoinformatics-neoantigen-prediction/SKILL.md +277 -0
- package/data/skills/bio-immunoinformatics-tcr-epitope-binding/SKILL.md +257 -0
- package/data/skills/bio-isoform-switching/SKILL.md +192 -0
- package/data/skills/bio-liquid-biopsy-pipeline/SKILL.md +311 -0
- package/data/skills/bio-local-blast/SKILL.md +350 -0
- package/data/skills/bio-long-read-sequencing-clair3-variants/SKILL.md +252 -0
- package/data/skills/bio-long-read-sequencing-isoseq-analysis/SKILL.md +334 -0
- package/data/skills/bio-long-read-sequencing-nanopore-methylation/SKILL.md +110 -0
- package/data/skills/bio-longitudinal-monitoring/SKILL.md +271 -0
- package/data/skills/bio-longread-alignment/SKILL.md +193 -0
- package/data/skills/bio-longread-medaka/SKILL.md +176 -0
- package/data/skills/bio-longread-qc/SKILL.md +224 -0
- package/data/skills/bio-longread-structural-variants/SKILL.md +201 -0
- package/data/skills/bio-machine-learning-atlas-mapping/SKILL.md +139 -0
- package/data/skills/bio-machine-learning-biomarker-discovery/SKILL.md +157 -0
- package/data/skills/bio-machine-learning-model-validation/SKILL.md +148 -0
- package/data/skills/bio-machine-learning-omics-classifiers/SKILL.md +146 -0
- package/data/skills/bio-machine-learning-prediction-explanation/SKILL.md +162 -0
- package/data/skills/bio-machine-learning-survival-analysis/SKILL.md +176 -0
- package/data/skills/bio-metabolomics-lipidomics/SKILL.md +265 -0
- package/data/skills/bio-metabolomics-metabolite-annotation/SKILL.md +241 -0
- package/data/skills/bio-metabolomics-msdial-preprocessing/SKILL.md +308 -0
- package/data/skills/bio-metabolomics-normalization-qc/SKILL.md +283 -0
- package/data/skills/bio-metabolomics-pathway-mapping/SKILL.md +237 -0
- package/data/skills/bio-metabolomics-statistical-analysis/SKILL.md +276 -0
- package/data/skills/bio-metabolomics-targeted-analysis/SKILL.md +314 -0
- package/data/skills/bio-metabolomics-xcms-preprocessing/SKILL.md +268 -0
- package/data/skills/bio-metagenomics-abundance/SKILL.md +203 -0
- package/data/skills/bio-metagenomics-amr-detection/SKILL.md +293 -0
- package/data/skills/bio-metagenomics-functional-profiling/SKILL.md +252 -0
- package/data/skills/bio-metagenomics-kraken/SKILL.md +204 -0
- package/data/skills/bio-metagenomics-metaphlan/SKILL.md +214 -0
- package/data/skills/bio-metagenomics-strain-tracking/SKILL.md +292 -0
- package/data/skills/bio-metagenomics-visualization/SKILL.md +240 -0
- package/data/skills/bio-methylation-based-detection/SKILL.md +223 -0
- package/data/skills/bio-methylation-bismark-alignment/SKILL.md +195 -0
- package/data/skills/bio-methylation-calling/SKILL.md +200 -0
- package/data/skills/bio-methylation-dmr-detection/SKILL.md +211 -0
- package/data/skills/bio-methylation-methylkit/SKILL.md +219 -0
- package/data/skills/bio-microbiome-amplicon-processing/SKILL.md +137 -0
- package/data/skills/bio-microbiome-differential-abundance/SKILL.md +147 -0
- package/data/skills/bio-microbiome-diversity-analysis/SKILL.md +188 -0
- package/data/skills/bio-microbiome-functional-prediction/SKILL.md +153 -0
- package/data/skills/bio-microbiome-qiime2-workflow/SKILL.md +219 -0
- package/data/skills/bio-microbiome-taxonomy-assignment/SKILL.md +168 -0
- package/data/skills/bio-molecular-descriptors/SKILL.md +200 -0
- package/data/skills/bio-molecular-io/SKILL.md +188 -0
- package/data/skills/bio-motif-search/SKILL.md +354 -0
- package/data/skills/bio-multi-omics-data-harmonization/SKILL.md +228 -0
- package/data/skills/bio-multi-omics-mixomics-analysis/SKILL.md +221 -0
- package/data/skills/bio-multi-omics-mofa-integration/SKILL.md +225 -0
- package/data/skills/bio-multi-omics-similarity-network/SKILL.md +235 -0
- package/data/skills/bio-orchestrator/SKILL.md +133 -0
- package/data/skills/bio-paired-end-fastq/SKILL.md +334 -0
- package/data/skills/bio-pathway-enrichment-visualization/SKILL.md +278 -0
- package/data/skills/bio-pathway-go-enrichment/SKILL.md +218 -0
- package/data/skills/bio-pathway-gsea/SKILL.md +227 -0
- package/data/skills/bio-pathway-kegg-pathways/SKILL.md +234 -0
- package/data/skills/bio-pathway-reactome/SKILL.md +215 -0
- package/data/skills/bio-pathway-wikipathways/SKILL.md +255 -0
- package/data/skills/bio-pdb-geometric-analysis/SKILL.md +475 -0
- package/data/skills/bio-pdb-structure-io/SKILL.md +296 -0
- package/data/skills/bio-pdb-structure-modification/SKILL.md +448 -0
- package/data/skills/bio-pdb-structure-navigation/SKILL.md +335 -0
- package/data/skills/bio-phasing-imputation-genotype-imputation/SKILL.md +201 -0
- package/data/skills/bio-phasing-imputation-haplotype-phasing/SKILL.md +190 -0
- package/data/skills/bio-phasing-imputation-imputation-qc/SKILL.md +265 -0
- package/data/skills/bio-phasing-imputation-reference-panels/SKILL.md +203 -0
- package/data/skills/bio-phylo-distance-calculations/SKILL.md +307 -0
- package/data/skills/bio-phylo-modern-tree-inference/SKILL.md +274 -0
- package/data/skills/bio-phylo-tree-io/SKILL.md +252 -0
- package/data/skills/bio-phylo-tree-manipulation/SKILL.md +375 -0
- package/data/skills/bio-phylo-tree-visualization/SKILL.md +275 -0
- package/data/skills/bio-pileup-generation/SKILL.md +314 -0
- package/data/skills/bio-population-genetics-association-testing/SKILL.md +293 -0
- package/data/skills/bio-population-genetics-linkage-disequilibrium/SKILL.md +260 -0
- package/data/skills/bio-population-genetics-plink-basics/SKILL.md +338 -0
- package/data/skills/bio-population-genetics-population-structure/SKILL.md +352 -0
- package/data/skills/bio-population-genetics-scikit-allel-analysis/SKILL.md +306 -0
- package/data/skills/bio-population-genetics-selection-statistics/SKILL.md +251 -0
- package/data/skills/bio-primer-design-primer-basics/SKILL.md +289 -0
- package/data/skills/bio-primer-design-primer-validation/SKILL.md +344 -0
- package/data/skills/bio-primer-design-qpcr-primers/SKILL.md +273 -0
- package/data/skills/bio-proteomics-data-import/SKILL.md +122 -0
- package/data/skills/bio-proteomics-dia-analysis/SKILL.md +246 -0
- package/data/skills/bio-proteomics-differential-abundance/SKILL.md +129 -0
- package/data/skills/bio-proteomics-peptide-identification/SKILL.md +122 -0
- package/data/skills/bio-proteomics-protein-inference/SKILL.md +174 -0
- package/data/skills/bio-proteomics-proteomics-qc/SKILL.md +208 -0
- package/data/skills/bio-proteomics-ptm-analysis/SKILL.md +139 -0
- package/data/skills/bio-proteomics-quantification/SKILL.md +141 -0
- package/data/skills/bio-proteomics-spectral-libraries/SKILL.md +270 -0
- package/data/skills/bio-reaction-enumeration/SKILL.md +251 -0
- package/data/skills/bio-read-alignment-bowtie2-alignment/SKILL.md +189 -0
- package/data/skills/bio-read-alignment-bwa-alignment/SKILL.md +166 -0
- package/data/skills/bio-read-alignment-hisat2-alignment/SKILL.md +205 -0
- package/data/skills/bio-read-alignment-star-alignment/SKILL.md +204 -0
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- package/data/workflows/pcr-primer-design/SKILL.md +397 -0
- package/data/workflows/pcr-primer-design/references/code_examples.md +594 -0
- package/data/workflows/pcr-primer-design/references/miqe_guidelines.md +453 -0
- package/data/workflows/pcr-primer-design/references/parameter_ranges.md +356 -0
- package/data/workflows/pcr-primer-design/references/primer_design_best_practices.md +451 -0
- package/data/workflows/pcr-primer-design/references/troubleshooting_guide.md +477 -0
- package/data/workflows/pcr-primer-design/scripts/__init__.py +2 -0
- package/data/workflows/pcr-primer-design/scripts/calculate_tm.py +306 -0
- package/data/workflows/pcr-primer-design/scripts/check_dimers.py +298 -0
- package/data/workflows/pcr-primer-design/scripts/check_secondary_structures.py +343 -0
- package/data/workflows/pcr-primer-design/scripts/design_qpcr_primers.py +233 -0
- package/data/workflows/pcr-primer-design/scripts/design_standard_primers.py +197 -0
- package/data/workflows/pcr-primer-design/scripts/design_taqman_probes.py +226 -0
- package/data/workflows/pcr-primer-design/scripts/export_results.py +382 -0
- package/data/workflows/pcr-primer-design/scripts/generate_reports.py +379 -0
- package/data/workflows/pcr-primer-design/scripts/validate_specificity.py +311 -0
- package/data/workflows/pcr-primer-design/scripts/visualize_primers.py +379 -0
- package/data/workflows/polygenic-risk-score-prs-catalog/SKILL.md +195 -0
- package/data/workflows/polygenic-risk-score-prs-catalog/references/interpretation-guide.md +80 -0
- package/data/workflows/polygenic-risk-score-prs-catalog/references/pgs-catalog-guide.md +109 -0
- package/data/workflows/polygenic-risk-score-prs-catalog/scripts/export_results.R +186 -0
- package/data/workflows/polygenic-risk-score-prs-catalog/scripts/generate_plots.R +283 -0
- package/data/workflows/polygenic-risk-score-prs-catalog/scripts/load_pgs_weights.R +228 -0
- package/data/workflows/polygenic-risk-score-prs-catalog/scripts/load_reference_data.R +191 -0
- package/data/workflows/polygenic-risk-score-prs-catalog/scripts/score_traits.R +216 -0
- package/data/workflows/pooled-crispr-screens/SKILL.md +362 -0
- package/data/workflows/pooled-crispr-screens/references/crispr_screen_best_practices.md +349 -0
- package/data/workflows/pooled-crispr-screens/references/qc_guidelines.md +722 -0
- package/data/workflows/pooled-crispr-screens/references/statistical_methods.md +644 -0
- package/data/workflows/pooled-crispr-screens/references/troubleshooting_guide.md +684 -0
- package/data/workflows/pooled-crispr-screens/references/umi_optimization.md +297 -0
- package/data/workflows/pooled-crispr-screens/scripts/concatenate_libraries.py +132 -0
- package/data/workflows/pooled-crispr-screens/scripts/detect_perturbed_cells.py +255 -0
- package/data/workflows/pooled-crispr-screens/scripts/differential_expression.py +202 -0
- package/data/workflows/pooled-crispr-screens/scripts/differential_expression_glmgampoi.py +320 -0
- package/data/workflows/pooled-crispr-screens/scripts/export_results.py +261 -0
- package/data/workflows/pooled-crispr-screens/scripts/expression_filtering.py +159 -0
- package/data/workflows/pooled-crispr-screens/scripts/gene_name_corrections.py +188 -0
- package/data/workflows/pooled-crispr-screens/scripts/generate_report.py +485 -0
- package/data/workflows/pooled-crispr-screens/scripts/load_10x_libraries.py +69 -0
- package/data/workflows/pooled-crispr-screens/scripts/load_example_data.py +257 -0
- package/data/workflows/pooled-crispr-screens/scripts/map_sgrna_to_cells.py +119 -0
- package/data/workflows/pooled-crispr-screens/scripts/normalize_and_scale.py +140 -0
- package/data/workflows/pooled-crispr-screens/scripts/qc_filtering.py +185 -0
- package/data/workflows/pooled-crispr-screens/scripts/run_glmgampoi.R +181 -0
- package/data/workflows/pooled-crispr-screens/scripts/screen_all_perturbations.py +306 -0
- package/data/workflows/pooled-crispr-screens/scripts/validate_perturbations.py +314 -0
- package/data/workflows/pooled-crispr-screens/scripts/visualize_perturbations.py +314 -0
- package/data/workflows/scrnaseq-scanpy-core-analysis/SKILL.md +425 -0
- package/data/workflows/scrnaseq-scanpy-core-analysis/references/ambient_rna_correction.md +422 -0
- package/data/workflows/scrnaseq-scanpy-core-analysis/references/common-patterns.md +533 -0
- package/data/workflows/scrnaseq-scanpy-core-analysis/references/integration_methods.md +820 -0
- package/data/workflows/scrnaseq-scanpy-core-analysis/references/marker_gene_database.md +471 -0
- package/data/workflows/scrnaseq-scanpy-core-analysis/references/pseudobulk_de_guide.md +408 -0
- package/data/workflows/scrnaseq-scanpy-core-analysis/references/qc_guidelines.md +535 -0
- package/data/workflows/scrnaseq-scanpy-core-analysis/references/scanpy_best_practices.md +496 -0
- package/data/workflows/scrnaseq-scanpy-core-analysis/references/troubleshooting_guide.md +668 -0
- package/data/workflows/scrnaseq-scanpy-core-analysis/references/workflow-details.md +727 -0
- package/data/workflows/scrnaseq-scanpy-core-analysis/scripts/annotate_celltypes.py +431 -0
- package/data/workflows/scrnaseq-scanpy-core-analysis/scripts/cluster_cells.py +293 -0
- package/data/workflows/scrnaseq-scanpy-core-analysis/scripts/export_results.py +423 -0
- package/data/workflows/scrnaseq-scanpy-core-analysis/scripts/filter_cells.py +531 -0
- package/data/workflows/scrnaseq-scanpy-core-analysis/scripts/find_markers.py +391 -0
- package/data/workflows/scrnaseq-scanpy-core-analysis/scripts/find_variable_genes.py +222 -0
- package/data/workflows/scrnaseq-scanpy-core-analysis/scripts/integrate_scvi.py +665 -0
- package/data/workflows/scrnaseq-scanpy-core-analysis/scripts/integration_diagnostics.py +678 -0
- package/data/workflows/scrnaseq-scanpy-core-analysis/scripts/load_example_data.py +68 -0
- package/data/workflows/scrnaseq-scanpy-core-analysis/scripts/normalize_data.py +325 -0
- package/data/workflows/scrnaseq-scanpy-core-analysis/scripts/plot_dimreduction.py +389 -0
- package/data/workflows/scrnaseq-scanpy-core-analysis/scripts/plot_qc.py +320 -0
- package/data/workflows/scrnaseq-scanpy-core-analysis/scripts/pseudobulk_de.py +553 -0
- package/data/workflows/scrnaseq-scanpy-core-analysis/scripts/qc_metrics.py +477 -0
- package/data/workflows/scrnaseq-scanpy-core-analysis/scripts/remove_ambient_rna.py +347 -0
- package/data/workflows/scrnaseq-scanpy-core-analysis/scripts/run_umap.py +188 -0
- package/data/workflows/scrnaseq-scanpy-core-analysis/scripts/scale_and_pca.py +365 -0
- package/data/workflows/scrnaseq-scanpy-core-analysis/scripts/setup_and_import.py +334 -0
- package/data/workflows/scrnaseq-seurat-core-analysis/SKILL.md +585 -0
- package/data/workflows/scrnaseq-seurat-core-analysis/references/ambient_rna_correction.md +422 -0
- package/data/workflows/scrnaseq-seurat-core-analysis/references/common-patterns.md +667 -0
- package/data/workflows/scrnaseq-seurat-core-analysis/references/decision-guide.md +456 -0
- package/data/workflows/scrnaseq-seurat-core-analysis/references/integration_methods.md +864 -0
- package/data/workflows/scrnaseq-seurat-core-analysis/references/marker_gene_database.md +471 -0
- package/data/workflows/scrnaseq-seurat-core-analysis/references/pseudobulk_de_guide.md +408 -0
- package/data/workflows/scrnaseq-seurat-core-analysis/references/qc_guidelines.md +452 -0
- package/data/workflows/scrnaseq-seurat-core-analysis/references/seurat_best_practices.md +417 -0
- package/data/workflows/scrnaseq-seurat-core-analysis/references/troubleshooting_guide.md +566 -0
- package/data/workflows/scrnaseq-seurat-core-analysis/references/workflow-details.md +801 -0
- package/data/workflows/scrnaseq-seurat-core-analysis/scripts/annotate_celltypes.R +306 -0
- package/data/workflows/scrnaseq-seurat-core-analysis/scripts/cluster_cells.R +223 -0
- package/data/workflows/scrnaseq-seurat-core-analysis/scripts/export_results.R +292 -0
- package/data/workflows/scrnaseq-seurat-core-analysis/scripts/filter_cells.R +576 -0
- package/data/workflows/scrnaseq-seurat-core-analysis/scripts/find_markers.R +325 -0
- package/data/workflows/scrnaseq-seurat-core-analysis/scripts/find_variable_features.R +106 -0
- package/data/workflows/scrnaseq-seurat-core-analysis/scripts/integrate_batches.R +504 -0
- package/data/workflows/scrnaseq-seurat-core-analysis/scripts/integration_diagnostics.R +596 -0
- package/data/workflows/scrnaseq-seurat-core-analysis/scripts/load_example_data.R +89 -0
- package/data/workflows/scrnaseq-seurat-core-analysis/scripts/normalize_data.R +184 -0
- package/data/workflows/scrnaseq-seurat-core-analysis/scripts/plot_dimreduction.R +273 -0
- package/data/workflows/scrnaseq-seurat-core-analysis/scripts/plot_qc.R +250 -0
- package/data/workflows/scrnaseq-seurat-core-analysis/scripts/pseudobulk_de.R +324 -0
- package/data/workflows/scrnaseq-seurat-core-analysis/scripts/qc_metrics.R +358 -0
- package/data/workflows/scrnaseq-seurat-core-analysis/scripts/remove_ambient_rna.R +281 -0
- package/data/workflows/scrnaseq-seurat-core-analysis/scripts/run_umap.R +116 -0
- package/data/workflows/scrnaseq-seurat-core-analysis/scripts/scale_and_pca.R +243 -0
- package/data/workflows/scrnaseq-seurat-core-analysis/scripts/setup_and_import.R +193 -0
- package/data/workflows/spatial-transcriptomics/SKILL.md +256 -0
- package/data/workflows/spatial-transcriptomics/references/spatial-analysis-guide.md +216 -0
- package/data/workflows/spatial-transcriptomics/scripts/export_results.py +214 -0
- package/data/workflows/spatial-transcriptomics/scripts/generate_all_plots.py +397 -0
- package/data/workflows/spatial-transcriptomics/scripts/load_example_data.py +175 -0
- package/data/workflows/spatial-transcriptomics/scripts/spatial_workflow.py +206 -0
- package/dist/bgi.js +128 -2
- package/package.json +2 -1
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"""
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============================================================================
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LOAD SPATIAL TRANSCRIPTOMICS DATA
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============================================================================
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Functions:
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- load_visium_heart(): Load V1_Human_Heart example dataset from 10x Genomics
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- load_visium_data(): Load user-provided Visium data (h5ad or Space Ranger)
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Usage:
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from load_example_data import load_visium_heart
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adata = load_visium_heart()
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"""
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from pathlib import Path
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from typing import Optional, Union
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def load_visium_heart() -> 'anndata.AnnData':
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"""
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Load V1_Human_Heart Visium dataset from 10x Genomics.
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Downloads the processed dataset via scanpy's built-in datasets module.
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Contains ~4,247 spots and ~33,538 genes with H&E tissue image.
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Returns
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-------
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AnnData
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Visium spatial transcriptomics data with:
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- .X: count matrix (spots x genes)
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- .obs: spot metadata
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"""
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import scanpy as sc
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print("Loading V1_Human_Heart Visium dataset...")
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print(" Source: 10x Genomics Spatial Gene Expression")
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print(" Description: Human heart tissue section")
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print(" Platform: Visium Spatial Gene Expression")
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print(" (First run downloads ~50 MB, subsequent runs use cache)")
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try:
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adata = sc.datasets.visium_sge(sample_id="V1_Human_Heart")
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except AttributeError:
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# Fallback for Python <3.12 (tarfile.data_filter not available)
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print(" Using manual download fallback...")
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adata = _download_visium_manual("V1_Human_Heart")
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# Make variable names unique (critical for some Visium datasets)
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adata.var_names_make_unique()
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# Report dataset summary
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has_image = 'spatial' in adata.uns and len(adata.uns['spatial']) > 0
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print(f"\n✓ Data loaded successfully!")
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print(f" Spots: {adata.n_obs}")
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print(f" Genes: {adata.n_vars}")
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print(f" Spatial image: {'Yes' if has_image else 'No'}")
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print(f" Spatial coordinates: {adata.obsm['spatial'].shape}")
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return adata
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def _download_file(url: str, dest: Path) -> None:
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"""Download a file with proper User-Agent header."""
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req = urllib.request.Request(url, headers={
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'User-Agent': 'Mozilla/5.0 (compatible; scanpy-downloader)'
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})
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with urllib.request.urlopen(req) as response, open(dest, 'wb') as out:
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def _download_visium_manual(sample_id: str) -> 'anndata.AnnData':
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"""Manual Visium dataset download for Python <3.12 compatibility."""
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import scanpy as sc
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import tarfile
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base_url = "https://cf.10xgenomics.com/samples/spatial-exp/1.1.0"
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cache_dir = Path(sc.settings.datasetdir) / sample_id
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cache_dir.mkdir(parents=True, exist_ok=True)
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# Download H5 file (required by sc.read_visium)
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h5_path = cache_dir / "filtered_feature_bc_matrix.h5"
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if not h5_path.exists():
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h5_url = f"{base_url}/{sample_id}/{sample_id}_filtered_feature_bc_matrix.h5"
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print(f" Downloading filtered feature-barcode matrix (H5)...")
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_download_file(h5_url, h5_path)
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# Download and extract spatial data
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spatial_dir = cache_dir / "spatial"
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if not spatial_dir.exists():
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spatial_url = f"{base_url}/{sample_id}/{sample_id}_spatial.tar.gz"
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print(f" Downloading spatial data...")
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tar_path = cache_dir / "spatial.tar.gz"
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_download_file(spatial_url, tar_path)
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with tarfile.open(tar_path, 'r:gz') as tf:
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tf.extractall(cache_dir)
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tar_path.unlink()
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adata = sc.read_visium(cache_dir)
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return adata
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def load_visium_data(
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path: Union[str, Path],
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library_id: Optional[str] = None
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) -> 'anndata.AnnData':
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"""
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Load user-provided Visium data.
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Supports:
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- .h5ad files (AnnData format)
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- Space Ranger output directory (with filtered_feature_bc_matrix/)
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- .h5 files (10x HDF5 format)
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Parameters
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----------
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path : str or Path
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Path to data file or Space Ranger output directory.
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library_id : str, optional
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Library ID for Space Ranger output.
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Returns
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-------
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AnnData
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Loaded spatial data with spatial coordinates.
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"""
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import scanpy as sc
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path = Path(path)
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if not path.exists():
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raise FileNotFoundError(f"Path not found: {path}")
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if path.suffix == '.h5ad':
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print(f"Loading AnnData from {path}...")
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adata = sc.read_h5ad(path)
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elif path.suffix == '.h5':
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print(f"Loading 10x HDF5 from {path}...")
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adata = sc.read_10x_h5(path)
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elif path.is_dir():
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print(f"Loading Space Ranger output from {path}...")
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adata = sc.read_visium(path, library_id=library_id)
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else:
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raise ValueError(
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f"Unsupported format: {path.suffix}. "
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"Expected .h5ad, .h5, or Space Ranger output directory."
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)
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adata.var_names_make_unique()
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has_image = 'spatial' in adata.uns and len(adata.uns['spatial']) > 0
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print(f"\n✓ Data loaded successfully!")
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print(f" Spots: {adata.n_obs}")
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print(f" Genes: {adata.n_vars}")
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print(f" Spatial image: {'Yes' if has_image else 'No'}")
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if 'spatial' in adata.obsm:
|
|
168
|
+
print(f" Spatial coordinates: {adata.obsm['spatial'].shape}")
|
|
169
|
+
|
|
170
|
+
return adata
|
|
171
|
+
|
|
172
|
+
|
|
173
|
+
if __name__ == "__main__":
|
|
174
|
+
adata = load_visium_heart()
|
|
175
|
+
print("\nExample data ready for spatial analysis!")
|
|
@@ -0,0 +1,206 @@
|
|
|
1
|
+
"""
|
|
2
|
+
============================================================================
|
|
3
|
+
SPATIAL TRANSCRIPTOMICS ANALYSIS WORKFLOW
|
|
4
|
+
============================================================================
|
|
5
|
+
|
|
6
|
+
Complete Visium spatial analysis: QC -> filtering -> normalization ->
|
|
7
|
+
clustering -> spatial neighbors -> SVGs -> neighborhood enrichment ->
|
|
8
|
+
co-occurrence.
|
|
9
|
+
|
|
10
|
+
Functions:
|
|
11
|
+
- run_spatial_analysis(): Execute complete spatial workflow
|
|
12
|
+
|
|
13
|
+
Usage:
|
|
14
|
+
from spatial_workflow import run_spatial_analysis
|
|
15
|
+
adata = run_spatial_analysis(adata)
|
|
16
|
+
"""
|
|
17
|
+
|
|
18
|
+
import numpy as np
|
|
19
|
+
import pandas as pd
|
|
20
|
+
from typing import Optional, List
|
|
21
|
+
|
|
22
|
+
|
|
23
|
+
def run_spatial_analysis(
|
|
24
|
+
adata: 'anndata.AnnData',
|
|
25
|
+
min_genes: int = 200,
|
|
26
|
+
min_cells: int = 10,
|
|
27
|
+
max_pct_mito: float = 50.0,
|
|
28
|
+
n_top_genes: int = 2000,
|
|
29
|
+
n_pcs: int = 30,
|
|
30
|
+
resolution: float = 0.8,
|
|
31
|
+
n_neighbors: int = 15,
|
|
32
|
+
svgs_n_perms: int = 100,
|
|
33
|
+
svgs_n_jobs: int = 1,
|
|
34
|
+
output_dir: str = "visium_results"
|
|
35
|
+
) -> 'anndata.AnnData':
|
|
36
|
+
"""
|
|
37
|
+
Run complete spatial transcriptomics analysis.
|
|
38
|
+
|
|
39
|
+
Pipeline:
|
|
40
|
+
1. QC metrics (mitochondrial %, total counts, genes per spot)
|
|
41
|
+
2. Filter spots (min_genes, max_mito)
|
|
42
|
+
3. Normalize + log1p
|
|
43
|
+
4. HVG selection (seurat_v3 on raw counts)
|
|
44
|
+
5. PCA + Neighbors + UMAP + Leiden clustering
|
|
45
|
+
6. Spatial neighbors graph
|
|
46
|
+
7. Spatially variable genes (Moran's I)
|
|
47
|
+
8. Neighborhood enrichment
|
|
48
|
+
9. Co-occurrence analysis
|
|
49
|
+
|
|
50
|
+
Parameters
|
|
51
|
+
----------
|
|
52
|
+
adata : AnnData
|
|
53
|
+
Raw Visium data (from load_example_data).
|
|
54
|
+
min_genes : int
|
|
55
|
+
Minimum genes per spot (default: 200).
|
|
56
|
+
min_cells : int
|
|
57
|
+
Minimum spots per gene (default: 10).
|
|
58
|
+
max_pct_mito : float
|
|
59
|
+
Maximum mitochondrial percentage (default: 50.0; heart tissue is MT-rich).
|
|
60
|
+
n_top_genes : int
|
|
61
|
+
Number of highly variable genes (default: 2000).
|
|
62
|
+
n_pcs : int
|
|
63
|
+
Number of PCA components (default: 30).
|
|
64
|
+
resolution : float
|
|
65
|
+
Leiden clustering resolution (default: 0.8).
|
|
66
|
+
n_neighbors : int
|
|
67
|
+
Number of neighbors for expression graph (default: 15).
|
|
68
|
+
svgs_n_perms : int
|
|
69
|
+
Permutations for Moran's I (default: 100).
|
|
70
|
+
svgs_n_jobs : int
|
|
71
|
+
Parallel jobs for Moran's I (default: 1).
|
|
72
|
+
output_dir : str
|
|
73
|
+
Output directory (default: "visium_results").
|
|
74
|
+
|
|
75
|
+
Returns
|
|
76
|
+
-------
|
|
77
|
+
AnnData
|
|
78
|
+
Processed adata with clustering, SVGs, enrichment results attached.
|
|
79
|
+
"""
|
|
80
|
+
import scanpy as sc
|
|
81
|
+
import squidpy as sq
|
|
82
|
+
import os
|
|
83
|
+
|
|
84
|
+
os.makedirs(output_dir, exist_ok=True)
|
|
85
|
+
|
|
86
|
+
print("\n=== Step 2: Spatial Transcriptomics Analysis ===\n")
|
|
87
|
+
n_spots_initial = adata.n_obs
|
|
88
|
+
|
|
89
|
+
# --- 1. QC metrics ---
|
|
90
|
+
print("1. Calculating QC metrics...")
|
|
91
|
+
adata.var['mt'] = adata.var_names.str.startswith('MT-')
|
|
92
|
+
sc.pp.calculate_qc_metrics(
|
|
93
|
+
adata, qc_vars=['mt'], percent_top=None, log1p=False, inplace=True
|
|
94
|
+
)
|
|
95
|
+
print(f" Median genes/spot: {adata.obs['n_genes_by_counts'].median():.0f}")
|
|
96
|
+
print(f" Median counts/spot: {adata.obs['total_counts'].median():.0f}")
|
|
97
|
+
print(f" Median %MT: {adata.obs['pct_counts_mt'].median():.1f}%")
|
|
98
|
+
|
|
99
|
+
# --- 2. Filter spots ---
|
|
100
|
+
print(f"\n2. Filtering spots (min_genes={min_genes}, max_mito={max_pct_mito}%)...")
|
|
101
|
+
|
|
102
|
+
# Store raw counts BEFORE any processing
|
|
103
|
+
adata.layers['counts'] = adata.X.copy()
|
|
104
|
+
|
|
105
|
+
sc.pp.filter_cells(adata, min_genes=min_genes)
|
|
106
|
+
sc.pp.filter_genes(adata, min_cells=min_cells)
|
|
107
|
+
adata = adata[adata.obs['pct_counts_mt'] < max_pct_mito, :].copy()
|
|
108
|
+
|
|
109
|
+
n_spots_after = adata.n_obs
|
|
110
|
+
retention = 100 * n_spots_after / n_spots_initial
|
|
111
|
+
print(f" Spots: {n_spots_initial} -> {n_spots_after} ({retention:.1f}% retained)")
|
|
112
|
+
|
|
113
|
+
# --- 3. Normalize ---
|
|
114
|
+
print("\n3. Normalizing (target sum + log1p)...")
|
|
115
|
+
sc.pp.normalize_total(adata, target_sum=1e4)
|
|
116
|
+
sc.pp.log1p(adata)
|
|
117
|
+
|
|
118
|
+
# Store normalized counts for SVG computation later
|
|
119
|
+
adata.layers['normalized'] = adata.X.copy()
|
|
120
|
+
|
|
121
|
+
# --- 4. HVG selection ---
|
|
122
|
+
print(f"\n4. Selecting {n_top_genes} highly variable genes...")
|
|
123
|
+
sc.pp.highly_variable_genes(
|
|
124
|
+
adata, n_top_genes=n_top_genes, flavor='seurat_v3',
|
|
125
|
+
layer='counts'
|
|
126
|
+
)
|
|
127
|
+
n_hvgs = adata.var['highly_variable'].sum()
|
|
128
|
+
print(f" Highly variable genes: {n_hvgs}")
|
|
129
|
+
|
|
130
|
+
# --- 5. PCA + Neighbors + UMAP + Leiden ---
|
|
131
|
+
print("\n5. Dimensionality reduction and clustering...")
|
|
132
|
+
sc.pp.scale(adata, max_value=10)
|
|
133
|
+
sc.tl.pca(adata, n_comps=n_pcs, svd_solver='arpack')
|
|
134
|
+
sc.pp.neighbors(adata, n_neighbors=n_neighbors, n_pcs=n_pcs)
|
|
135
|
+
sc.tl.umap(adata)
|
|
136
|
+
sc.tl.leiden(adata, resolution=resolution, key_added='leiden')
|
|
137
|
+
|
|
138
|
+
n_clusters = adata.obs['leiden'].nunique()
|
|
139
|
+
print(f" PCA: {n_pcs} components")
|
|
140
|
+
print(f" Leiden clusters: {n_clusters} (resolution={resolution})")
|
|
141
|
+
|
|
142
|
+
# --- 6. Spatial neighbors ---
|
|
143
|
+
print("\n6. Building spatial neighbors graph...")
|
|
144
|
+
sq.gr.spatial_neighbors(adata, coord_type='grid', n_neighs=6)
|
|
145
|
+
n_edges = adata.obsp['spatial_connectivities'].nnz
|
|
146
|
+
print(f" Spatial graph: {n_edges} edges")
|
|
147
|
+
|
|
148
|
+
# --- 7. Spatially variable genes (Moran's I) ---
|
|
149
|
+
print(f"\n7. Computing spatially variable genes (Moran's I, n_perms={svgs_n_perms})...")
|
|
150
|
+
|
|
151
|
+
# Restore normalized expression for SVG computation (scale modifies X)
|
|
152
|
+
adata.X = adata.layers['normalized'].copy()
|
|
153
|
+
|
|
154
|
+
sq.gr.spatial_autocorr(
|
|
155
|
+
adata,
|
|
156
|
+
mode='moran',
|
|
157
|
+
n_perms=svgs_n_perms,
|
|
158
|
+
n_jobs=svgs_n_jobs
|
|
159
|
+
)
|
|
160
|
+
|
|
161
|
+
# Extract significant SVGs
|
|
162
|
+
moranI = adata.uns['moranI'].copy()
|
|
163
|
+
svgs = moranI[moranI['pval_norm_fdr_bh'] < 0.05].sort_values('I', ascending=False)
|
|
164
|
+
n_svgs = len(svgs)
|
|
165
|
+
print(f" Spatially variable genes (FDR < 0.05): {n_svgs}")
|
|
166
|
+
if n_svgs > 0:
|
|
167
|
+
top5 = svgs.index[:5].tolist()
|
|
168
|
+
print(f" Top SVGs: {', '.join(top5)}")
|
|
169
|
+
|
|
170
|
+
# Store SVG results
|
|
171
|
+
adata.uns['svg_results'] = svgs
|
|
172
|
+
|
|
173
|
+
# --- 8. Neighborhood enrichment ---
|
|
174
|
+
print("\n8. Computing neighborhood enrichment...")
|
|
175
|
+
sq.gr.nhood_enrichment(adata, cluster_key='leiden')
|
|
176
|
+
print(f" Enrichment matrix: {n_clusters}x{n_clusters}")
|
|
177
|
+
|
|
178
|
+
# --- 9. Co-occurrence ---
|
|
179
|
+
print("\n9. Computing co-occurrence scores...")
|
|
180
|
+
sq.gr.co_occurrence(adata, cluster_key='leiden')
|
|
181
|
+
print(f" Co-occurrence computed for {n_clusters} clusters")
|
|
182
|
+
|
|
183
|
+
# --- Store analysis parameters ---
|
|
184
|
+
adata.uns['spatial_analysis_params'] = {
|
|
185
|
+
'min_genes': min_genes,
|
|
186
|
+
'min_cells': min_cells,
|
|
187
|
+
'max_pct_mito': max_pct_mito,
|
|
188
|
+
'n_top_genes': n_top_genes,
|
|
189
|
+
'n_pcs': n_pcs,
|
|
190
|
+
'resolution': resolution,
|
|
191
|
+
'n_neighbors': n_neighbors,
|
|
192
|
+
'svgs_n_perms': svgs_n_perms,
|
|
193
|
+
'n_spots_initial': int(n_spots_initial),
|
|
194
|
+
'n_spots_after': int(n_spots_after),
|
|
195
|
+
'n_clusters': int(n_clusters),
|
|
196
|
+
'n_svgs': int(n_svgs),
|
|
197
|
+
}
|
|
198
|
+
|
|
199
|
+
print("\n" + "=" * 50)
|
|
200
|
+
print("✓ Spatial analysis completed successfully!")
|
|
201
|
+
print("=" * 50)
|
|
202
|
+
print(f"\n Spots analyzed: {n_spots_after}")
|
|
203
|
+
print(f" Clusters found: {n_clusters}")
|
|
204
|
+
print(f" SVGs identified: {n_svgs}")
|
|
205
|
+
|
|
206
|
+
return adata
|
package/dist/bgi.js
CHANGED
|
@@ -13930,6 +13930,33 @@ async function streamOnce(client, messages, model) {
|
|
|
13930
13930
|
finishReason
|
|
13931
13931
|
};
|
|
13932
13932
|
}
|
|
13933
|
+
async function compactMessages(messages, config) {
|
|
13934
|
+
const prov = PROVIDERS[config.provider];
|
|
13935
|
+
if (!prov) throw new Error(`Unknown provider: ${config.provider}`);
|
|
13936
|
+
const baseURL = config.provider === "custom" ? config.customUrl : prov.baseURL;
|
|
13937
|
+
const apiKey = getApiKey(config);
|
|
13938
|
+
const client = new openai_default({ apiKey: apiKey || "none", baseURL });
|
|
13939
|
+
const transcript = messages.filter((m2) => m2.role === "user" || m2.role === "assistant").map((m2) => `[${m2.role === "user" ? "\u7528\u6237" : "AI"}]: ${String(m2.content ?? "").slice(0, 2e3)}`).join("\n\n");
|
|
13940
|
+
const resp = await client.chat.completions.create({
|
|
13941
|
+
model: config.model,
|
|
13942
|
+
messages: [
|
|
13943
|
+
{
|
|
13944
|
+
role: "system",
|
|
13945
|
+
content: "\u4F60\u662F\u4E00\u4E2A\u5BF9\u8BDD\u6458\u8981\u52A9\u624B\u3002\u8BF7\u5C06\u4EE5\u4E0B\u5BF9\u8BDD\u5386\u53F2\u538B\u7F29\u4E3A\u7B80\u6D01\u7684\u4E2D\u6587\u6458\u8981\uFF0C\u4FDD\u7559\u6240\u6709\u5173\u952E\u6280\u672F\u4FE1\u606F\uFF1A\u6587\u4EF6\u8DEF\u5F84\u3001\u547D\u4EE4\u3001\u5206\u6790\u7ED3\u679C\u3001\u7528\u6237\u51B3\u7B56\u3001\u5DF2\u6FC0\u6D3B\u7684\u5DE5\u4F5C\u6D41/\u6280\u80FD\u3002\u6458\u8981\u5E94\u8BA9\u5BF9\u8BDD\u80FD\u591F\u65E0\u7F1D\u7EE7\u7EED\u3002"
|
|
13946
|
+
},
|
|
13947
|
+
{
|
|
13948
|
+
role: "user",
|
|
13949
|
+
content: `\u8BF7\u538B\u7F29\u4EE5\u4E0B\u5BF9\u8BDD\u5386\u53F2\uFF1A
|
|
13950
|
+
|
|
13951
|
+
${transcript}
|
|
13952
|
+
|
|
13953
|
+
\u8F93\u51FA\u683C\u5F0F\uFF1A\u76F4\u63A5\u8F93\u51FA\u6458\u8981\u6587\u672C\uFF0C\u4E0D\u9700\u8981\u4EFB\u4F55\u524D\u7F00\u3002`
|
|
13954
|
+
}
|
|
13955
|
+
],
|
|
13956
|
+
stream: false
|
|
13957
|
+
});
|
|
13958
|
+
return resp.choices[0]?.message?.content ?? "\uFF08\u5BF9\u8BDD\u5386\u53F2\u5DF2\u538B\u7F29\uFF09";
|
|
13959
|
+
}
|
|
13933
13960
|
function getApiKey(cfg) {
|
|
13934
13961
|
const prov = PROVIDERS[cfg.provider];
|
|
13935
13962
|
if (prov?.envKey && process.env[prov.envKey]) return process.env[prov.envKey];
|
|
@@ -14657,7 +14684,33 @@ function routeSkill(message) {
|
|
|
14657
14684
|
}
|
|
14658
14685
|
|
|
14659
14686
|
// src/index.ts
|
|
14660
|
-
var VERSION2 = "2.
|
|
14687
|
+
var VERSION2 = "2.2.0";
|
|
14688
|
+
function installBundledData() {
|
|
14689
|
+
const bundledData = (0, import_path4.join)(__dirname, "..", "data");
|
|
14690
|
+
if (!(0, import_fs4.existsSync)(bundledData)) return;
|
|
14691
|
+
ensureDirs();
|
|
14692
|
+
const targets = [
|
|
14693
|
+
{ src: (0, import_path4.join)(bundledData, "workflows"), dest: WORKFLOWS_DIR, name: "\u5DE5\u4F5C\u6D41" },
|
|
14694
|
+
{ src: (0, import_path4.join)(bundledData, "skills"), dest: SKILLS_DIR, name: "OpenClaw Skills" },
|
|
14695
|
+
{ src: (0, import_path4.join)(bundledData, "tools"), dest: TOOLS_DIR, name: "\u5DE5\u5177" }
|
|
14696
|
+
];
|
|
14697
|
+
let installed = false;
|
|
14698
|
+
for (const { src, dest, name } of targets) {
|
|
14699
|
+
if (!(0, import_fs4.existsSync)(src)) continue;
|
|
14700
|
+
const isEmpty = !(0, import_fs4.existsSync)(dest) || (0, import_fs4.readdirSync)(dest).length === 0;
|
|
14701
|
+
if (isEmpty) {
|
|
14702
|
+
(0, import_fs4.mkdirSync)(dest, { recursive: true });
|
|
14703
|
+
(0, import_fs4.cpSync)(src, dest, { recursive: true });
|
|
14704
|
+
if (!installed) {
|
|
14705
|
+
process.stdout.write(source_default.dim("\u6B63\u5728\u521D\u59CB\u5316\u5185\u7F6E\u6570\u636E...\n"));
|
|
14706
|
+
installed = true;
|
|
14707
|
+
}
|
|
14708
|
+
process.stdout.write(source_default.green(` \u2713 ${name} \u5DF2\u5B89\u88C5
|
|
14709
|
+
`));
|
|
14710
|
+
}
|
|
14711
|
+
}
|
|
14712
|
+
if (installed) console.log();
|
|
14713
|
+
}
|
|
14661
14714
|
function printBanner() {
|
|
14662
14715
|
console.log(source_default.cyan.bold(`
|
|
14663
14716
|
\u2588\u2588\u2588\u2588\u2588\u2588\u2557 \u2588\u2588\u2588\u2588\u2588\u2588\u2557 \u2588\u2588\u2557 \u2588\u2588\u2588\u2588\u2588\u2588\u2557\u2588\u2588\u2557 \u2588\u2588\u2557
|
|
@@ -14681,6 +14734,7 @@ function printHelp() {
|
|
|
14681
14734
|
console.log(source_default.bold.cyan("\u2500\u2500\u2500 \u5BF9\u8BDD\u7BA1\u7406 \u2500\u2500\u2500\u2500\u2500\u2500\u2500\u2500\u2500\u2500\u2500\u2500\u2500\u2500\u2500\u2500\u2500\u2500\u2500\u2500\u2500\u2500\u2500\u2500\u2500\u2500\u2500\u2500\u2500\u2500\u2500\u2500\u2500\u2500\u2500\u2500\u2500\u2500\u2500\u2500\u2500\u2500\u2500\u2500\u2500"));
|
|
14682
14735
|
console.log(` ${source_default.cyan("/clear")} \u6E05\u7A7A\u5BF9\u8BDD\u5386\u53F2`);
|
|
14683
14736
|
console.log(` ${source_default.cyan("/history")} \u67E5\u770B\u5BF9\u8BDD\u7EDF\u8BA1\uFF08\u8F6E\u6B21 / Token \u4F30\u7B97\uFF09`);
|
|
14737
|
+
console.log(` ${source_default.cyan("/compact")} \u7ACB\u5373\u538B\u7F29\u5BF9\u8BDD\u5386\u53F2\uFF08\u8D85 60k token \u81EA\u52A8\u89E6\u53D1\uFF09`);
|
|
14684
14738
|
console.log(` ${source_default.cyan("/save")} [\u6587\u4EF6\u540D] \u4FDD\u5B58\u5BF9\u8BDD\u4E3A Markdown \u6587\u4EF6`);
|
|
14685
14739
|
console.log(` ${source_default.cyan("/think")} [on|off] \u5207\u6362\u601D\u8003\u6A21\u5F0F (Qwen3 /think \u524D\u7F00)`);
|
|
14686
14740
|
console.log();
|
|
@@ -14908,6 +14962,45 @@ ${msg.content}
|
|
|
14908
14962
|
(0, import_fs4.writeFileSync)(outPath, lines.join("\n"), "utf8");
|
|
14909
14963
|
console.log(source_default.green(`\u2713 \u5BF9\u8BDD\u5DF2\u4FDD\u5B58: ${outPath}`));
|
|
14910
14964
|
}
|
|
14965
|
+
var COMPACT_TOKEN_THRESHOLD = 6e4;
|
|
14966
|
+
var COMPACT_KEEP_RECENT = 8;
|
|
14967
|
+
function estimateTokens(messages) {
|
|
14968
|
+
const chars = messages.reduce((n2, m2) => n2 + String(m2.content ?? "").length, 0);
|
|
14969
|
+
return Math.round(chars / 3.5);
|
|
14970
|
+
}
|
|
14971
|
+
async function maybeCompact(history, cfg) {
|
|
14972
|
+
const tokens = estimateTokens(history);
|
|
14973
|
+
if (tokens < COMPACT_TOKEN_THRESHOLD) return history;
|
|
14974
|
+
const recent = history.slice(-COMPACT_KEEP_RECENT);
|
|
14975
|
+
const old = history.slice(0, -COMPACT_KEEP_RECENT);
|
|
14976
|
+
if (old.length === 0) return history;
|
|
14977
|
+
process.stdout.write(source_default.dim(`
|
|
14978
|
+
[\u4E0A\u4E0B\u6587\u5DF2\u8FBE ~${Math.round(tokens / 1e3)}k tokens\uFF0C\u6B63\u5728\u81EA\u52A8\u538B\u7F29...]
|
|
14979
|
+
`));
|
|
14980
|
+
try {
|
|
14981
|
+
const summary = await compactMessages(old, cfg);
|
|
14982
|
+
const compacted = [
|
|
14983
|
+
{
|
|
14984
|
+
role: "user",
|
|
14985
|
+
content: `[\u5BF9\u8BDD\u5386\u53F2\u6458\u8981 \u2014 \u8BF7\u5728\u6B64\u57FA\u7840\u4E0A\u7EE7\u7EED]
|
|
14986
|
+
|
|
14987
|
+
${summary}`
|
|
14988
|
+
},
|
|
14989
|
+
{
|
|
14990
|
+
role: "assistant",
|
|
14991
|
+
content: "\u2713 \u5DF2\u7406\u89E3\u4E4B\u524D\u7684\u5BF9\u8BDD\u6458\u8981\uFF0C\u8BF7\u7EE7\u7EED\u3002"
|
|
14992
|
+
},
|
|
14993
|
+
...recent
|
|
14994
|
+
];
|
|
14995
|
+
const saved = estimateTokens(history) - estimateTokens(compacted);
|
|
14996
|
+
process.stdout.write(source_default.dim(`[\u538B\u7F29\u5B8C\u6210\uFF0C\u91CA\u653E\u7EA6 ~${Math.round(saved / 1e3)}k tokens]
|
|
14997
|
+
|
|
14998
|
+
`));
|
|
14999
|
+
return compacted;
|
|
15000
|
+
} catch {
|
|
15001
|
+
return history.slice(-COMPACT_KEEP_RECENT * 2);
|
|
15002
|
+
}
|
|
15003
|
+
}
|
|
14911
15004
|
async function handleCommand(input, rl, history, thinkMode) {
|
|
14912
15005
|
const [cmd, ...rest] = input.slice(1).trim().split(/\s+/);
|
|
14913
15006
|
const arg = rest.join(" ");
|
|
@@ -15011,6 +15104,37 @@ async function handleCommand(input, rl, history, thinkMode) {
|
|
|
15011
15104
|
saveConversation(history, arg || void 0);
|
|
15012
15105
|
break;
|
|
15013
15106
|
}
|
|
15107
|
+
case "compact": {
|
|
15108
|
+
const tokens = estimateTokens(history);
|
|
15109
|
+
if (history.length < 4) {
|
|
15110
|
+
console.log(source_default.dim("\u5BF9\u8BDD\u592A\u77ED\uFF0C\u65E0\u9700\u538B\u7F29"));
|
|
15111
|
+
break;
|
|
15112
|
+
}
|
|
15113
|
+
console.log(source_default.dim(`\u5F53\u524D\u5BF9\u8BDD\u7EA6 ~${Math.round(tokens / 1e3)}k tokens\uFF0C\u6B63\u5728\u538B\u7F29...`));
|
|
15114
|
+
try {
|
|
15115
|
+
const currentCfg = loadConfig();
|
|
15116
|
+
const recent = history.slice(-COMPACT_KEEP_RECENT);
|
|
15117
|
+
const old = history.slice(0, -COMPACT_KEEP_RECENT);
|
|
15118
|
+
if (old.length === 0) {
|
|
15119
|
+
console.log(source_default.dim("\u8FD1\u671F\u6D88\u606F\u4E0D\u8DB3\uFF0C\u65E0\u9700\u538B\u7F29"));
|
|
15120
|
+
break;
|
|
15121
|
+
}
|
|
15122
|
+
const summary = await compactMessages(old, currentCfg);
|
|
15123
|
+
const newHistory = [
|
|
15124
|
+
{ role: "user", content: `[\u5BF9\u8BDD\u5386\u53F2\u6458\u8981 \u2014 \u8BF7\u5728\u6B64\u57FA\u7840\u4E0A\u7EE7\u7EED]
|
|
15125
|
+
|
|
15126
|
+
${summary}` },
|
|
15127
|
+
{ role: "assistant", content: "\u2713 \u5DF2\u7406\u89E3\u4E4B\u524D\u7684\u5BF9\u8BDD\u6458\u8981\uFF0C\u8BF7\u7EE7\u7EED\u3002" },
|
|
15128
|
+
...recent
|
|
15129
|
+
];
|
|
15130
|
+
const after = estimateTokens(newHistory);
|
|
15131
|
+
console.log(source_default.green(`\u2713 \u538B\u7F29\u5B8C\u6210: ${history.length} \u6761\u6D88\u606F \u2192 ${newHistory.length} \u6761\uFF0C~${Math.round(after / 1e3)}k tokens`));
|
|
15132
|
+
return { injectHistory: newHistory };
|
|
15133
|
+
} catch (err) {
|
|
15134
|
+
console.error(source_default.red(`\u538B\u7F29\u5931\u8D25: ${err instanceof Error ? err.message : String(err)}`));
|
|
15135
|
+
}
|
|
15136
|
+
break;
|
|
15137
|
+
}
|
|
15014
15138
|
case "think": {
|
|
15015
15139
|
const val = arg.toLowerCase();
|
|
15016
15140
|
if (val === "on" || val === "1" || val === "true") {
|
|
@@ -15105,6 +15229,7 @@ async function handleCommand(input, rl, history, thinkMode) {
|
|
|
15105
15229
|
return {};
|
|
15106
15230
|
}
|
|
15107
15231
|
async function main() {
|
|
15232
|
+
installBundledData();
|
|
15108
15233
|
printBanner();
|
|
15109
15234
|
const rl = (0, import_readline.createInterface)({
|
|
15110
15235
|
input: process.stdin,
|
|
@@ -15155,6 +15280,7 @@ async function main() {
|
|
|
15155
15280
|
history = [];
|
|
15156
15281
|
injectedSkills.clear();
|
|
15157
15282
|
}
|
|
15283
|
+
if (result.injectHistory) history = result.injectHistory;
|
|
15158
15284
|
if (result.thinkMode !== void 0) thinkMode = result.thinkMode;
|
|
15159
15285
|
continue;
|
|
15160
15286
|
}
|
|
@@ -15183,7 +15309,7 @@ ${expanded}` : expanded;
|
|
|
15183
15309
|
const currentCfg = loadConfig();
|
|
15184
15310
|
const reply = await chat(history, currentCfg, systemPrompt);
|
|
15185
15311
|
history.push({ role: "assistant", content: reply });
|
|
15186
|
-
|
|
15312
|
+
history = await maybeCompact(history, currentCfg);
|
|
15187
15313
|
} catch (err) {
|
|
15188
15314
|
const msg = err instanceof Error ? err.message : String(err);
|
|
15189
15315
|
console.error(source_default.red(`
|
package/package.json
CHANGED
|
@@ -1,12 +1,13 @@
|
|
|
1
1
|
{
|
|
2
2
|
"name": "@bgicli/bgicli",
|
|
3
|
-
"version": "2.
|
|
3
|
+
"version": "2.2.1",
|
|
4
4
|
"description": "BGI CLI — Bioinformatics AI terminal for Chinese researchers (百炼/DeepSeek/Kimi/Qwen)",
|
|
5
5
|
"bin": {
|
|
6
6
|
"bgi": "dist/bgi.js"
|
|
7
7
|
},
|
|
8
8
|
"files": [
|
|
9
9
|
"dist/bgi.js",
|
|
10
|
+
"data/",
|
|
10
11
|
"README.md"
|
|
11
12
|
],
|
|
12
13
|
"publishConfig": {
|