seqtrimnext 2.0.36 → 2.0.38
This diff represents the content of publicly available package versions that have been released to one of the supported registries. The information contained in this diff is provided for informational purposes only and reflects changes between package versions as they appear in their respective public registries.
- data/History.txt +8 -0
- data/bin/seqtrimnext +9 -2
- data/lib/seqtrimnext/classes/seqtrim.rb +10 -2
- data/lib/seqtrimnext/plugins/plugin_adapters.rb +1 -1
- data/lib/seqtrimnext/plugins/plugin_contaminants.rb +2 -2
- data/lib/seqtrimnext/plugins/plugin_low_high_size.rb +2 -2
- data/lib/seqtrimnext/plugins/plugin_mids.rb +1 -1
- data/lib/seqtrimnext.rb +1 -1
- metadata +2 -2
data/History.txt
CHANGED
data/bin/seqtrimnext
CHANGED
@@ -205,6 +205,12 @@ optparse = OptionParser.new do |opts|
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opts.on( '-c', '--check_databases', 'Check Blast databases and reformat if necessary' ) do
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options[:check_db] = true
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end
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+
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# options[:skip_initial_stats] = false
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# opts.on( '-k', '--skip_initial_stats', 'Skip initial stats' ) do
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# options[:skip_initial_stats] = true
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# end
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options[:install_db] = nil
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opts.on( '-i', '--install_databases TYPE', 'Install base databases and reformat them if necessary') do |db_type|
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@@ -234,8 +240,9 @@ optparse = OptionParser.new do |opts|
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options[:list_db] = nil
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-
opts.on( '-L', '--list_db DB_NAME', 'List entries IDs in DB_NAME. Use "-L all" to view all available databases' ) do |value|
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-
options[:list_db] =
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opts.on( '-L', '--list_db [DB_NAME]', 'List entries IDs in DB_NAME. Use "-L all" to view all available databases' ) do |value|
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options[:list_db] = true
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options[:list_db_name] = value
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end
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options[:gen_params] = false
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@@ -94,6 +94,9 @@ class Seqtrim
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default_value='true'
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params.check_param(errors,'remove_clonality','String',default_value,comment)
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comment='Generate initial stats'
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default_value='true'
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params.check_param(errors,'generate_initial_stats','String',default_value,comment)
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comment='Minimum insert size for every trimmed sequence'
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default_value = 40
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@@ -208,9 +211,14 @@ class Seqtrim
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Dir.mkdir(OUTPUT_PATH)
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end
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-
$LOG.info "Calculatings stats"
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# Extract global stats
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-
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if params.get_param('generate_initial_stats')=='true'
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$LOG.info "Calculatings stats"
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+
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ExtractStats.new(sequence_reader,params)
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else
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$LOG.info "Skipping calculatings stats phase."
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end
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# save used params to file
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@@ -107,7 +107,7 @@ class PluginAdapters < Plugin
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actions=[]
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adapter_size=0
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-
@stats['adapter_size']={}
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# @stats['adapter_size']={}
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adapters.each do |ad| # adds the correspondent action to the sequence
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type = get_type_adapter(ad.q_beg,ad.q_end,seq)
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@@ -105,8 +105,8 @@ class PluginContaminants < Plugin
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contaminants_size=0
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# @stats[:contaminants_size]={}
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-
@stats['contaminants_size']={}
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-
@stats['rejected_seqs']={}
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# @stats['contaminants_size']={}
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# @stats['rejected_seqs']={}
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min_cont_size=@params.get_param('min_contam_seq_presence').to_i
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@@ -28,8 +28,8 @@ class PluginLowHighSize < Plugin
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max_size = @params.get_param('max_sequence_size_raw').to_i #max_size is: mean + 2dev
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#add_stats('rejected_seqs',seq.seq_fasta.length)
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actions=[]
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-
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if ((seq.seq_fasta.length>max_size) || (seq.seq_fasta.length<min_size)) #if length of sequence is out of (-2dev,2dev)
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+
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if ((max_size>0 && (seq.seq_fasta.length>max_size)) || (seq.seq_fasta.length<min_size)) #if length of sequence is out of (-2dev,2dev)
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$LOG.debug "#{seq.seq_name} rejected by size #{seq.seq_fasta.length} "
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type='ActionLowHighSize'
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# seq.add_action(0,seq.seq_fasta.length,type)
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@@ -101,7 +101,7 @@ class PluginMids < Plugin
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mid_initial_pos=mid.q_beg-mid.s_beg
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has_full_key=false
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if
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if !@params.get_param('sequencing_key').nil? && !@params.get_param('sequencing_key').empty?
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has_full_key = !seq.seq_fasta.index(@params.get_param('sequencing_key')).nil?
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end
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data/lib/seqtrimnext.rb
CHANGED
metadata
CHANGED
@@ -2,7 +2,7 @@
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name: seqtrimnext
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version: !ruby/object:Gem::Version
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prerelease:
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version: 2.0.
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version: 2.0.38
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platform: ruby
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authors:
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- Dario Guerrero & Almudena Bocinos
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@@ -10,7 +10,7 @@ autorequire:
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bindir: bin
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cert_chain: []
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date: 2011-
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date: 2011-07-07 00:00:00 Z
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dependencies:
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- !ruby/object:Gem::Dependency
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name: narray
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