seqtrimnext 2.0.36 → 2.0.38
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- data/History.txt +8 -0
- data/bin/seqtrimnext +9 -2
- data/lib/seqtrimnext/classes/seqtrim.rb +10 -2
- data/lib/seqtrimnext/plugins/plugin_adapters.rb +1 -1
- data/lib/seqtrimnext/plugins/plugin_contaminants.rb +2 -2
- data/lib/seqtrimnext/plugins/plugin_low_high_size.rb +2 -2
- data/lib/seqtrimnext/plugins/plugin_mids.rb +1 -1
- data/lib/seqtrimnext.rb +1 -1
- metadata +2 -2
data/History.txt
CHANGED
data/bin/seqtrimnext
CHANGED
@@ -205,6 +205,12 @@ optparse = OptionParser.new do |opts|
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205
205
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opts.on( '-c', '--check_databases', 'Check Blast databases and reformat if necessary' ) do
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206
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options[:check_db] = true
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end
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208
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+
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209
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+
# options[:skip_initial_stats] = false
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210
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+
# opts.on( '-k', '--skip_initial_stats', 'Skip initial stats' ) do
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211
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+
# options[:skip_initial_stats] = true
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212
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+
# end
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213
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+
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208
214
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209
215
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options[:install_db] = nil
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216
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opts.on( '-i', '--install_databases TYPE', 'Install base databases and reformat them if necessary') do |db_type|
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@@ -234,8 +240,9 @@ optparse = OptionParser.new do |opts|
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234
240
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235
241
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236
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options[:list_db] = nil
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237
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-
opts.on( '-L', '--list_db DB_NAME', 'List entries IDs in DB_NAME. Use "-L all" to view all available databases' ) do |value|
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238
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-
options[:list_db] =
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243
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+
opts.on( '-L', '--list_db [DB_NAME]', 'List entries IDs in DB_NAME. Use "-L all" to view all available databases' ) do |value|
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+
options[:list_db] = true
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245
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+
options[:list_db_name] = value
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end
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241
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options[:gen_params] = false
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@@ -94,6 +94,9 @@ class Seqtrim
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94
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default_value='true'
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95
95
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params.check_param(errors,'remove_clonality','String',default_value,comment)
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96
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97
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+
comment='Generate initial stats'
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98
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+
default_value='true'
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99
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+
params.check_param(errors,'generate_initial_stats','String',default_value,comment)
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97
100
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|
98
101
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comment='Minimum insert size for every trimmed sequence'
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99
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default_value = 40
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@@ -208,9 +211,14 @@ class Seqtrim
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208
211
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Dir.mkdir(OUTPUT_PATH)
|
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end
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213
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|
211
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-
$LOG.info "Calculatings stats"
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# Extract global stats
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213
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-
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215
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+
if params.get_param('generate_initial_stats')=='true'
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+
$LOG.info "Calculatings stats"
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+
|
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+
ExtractStats.new(sequence_reader,params)
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+
else
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220
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+
$LOG.info "Skipping calculatings stats phase."
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+
end
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214
222
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215
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# save used params to file
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@@ -107,7 +107,7 @@ class PluginAdapters < Plugin
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actions=[]
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adapter_size=0
|
110
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-
@stats['adapter_size']={}
|
110
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+
# @stats['adapter_size']={}
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adapters.each do |ad| # adds the correspondent action to the sequence
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type = get_type_adapter(ad.q_beg,ad.q_end,seq)
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@@ -105,8 +105,8 @@ class PluginContaminants < Plugin
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|
105
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contaminants_size=0
|
106
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|
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# @stats[:contaminants_size]={}
|
108
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-
@stats['contaminants_size']={}
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-
@stats['rejected_seqs']={}
|
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+
# @stats['contaminants_size']={}
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+
# @stats['rejected_seqs']={}
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min_cont_size=@params.get_param('min_contam_seq_presence').to_i
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@@ -28,8 +28,8 @@ class PluginLowHighSize < Plugin
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|
28
28
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max_size = @params.get_param('max_sequence_size_raw').to_i #max_size is: mean + 2dev
|
29
29
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#add_stats('rejected_seqs',seq.seq_fasta.length)
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actions=[]
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31
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-
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32
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-
if ((seq.seq_fasta.length>max_size) || (seq.seq_fasta.length<min_size)) #if length of sequence is out of (-2dev,2dev)
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31
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+
|
32
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+
if ((max_size>0 && (seq.seq_fasta.length>max_size)) || (seq.seq_fasta.length<min_size)) #if length of sequence is out of (-2dev,2dev)
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$LOG.debug "#{seq.seq_name} rejected by size #{seq.seq_fasta.length} "
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34
34
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type='ActionLowHighSize'
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35
35
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# seq.add_action(0,seq.seq_fasta.length,type)
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@@ -101,7 +101,7 @@ class PluginMids < Plugin
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|
101
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|
102
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mid_initial_pos=mid.q_beg-mid.s_beg
|
103
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has_full_key=false
|
104
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-
if
|
104
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+
if !@params.get_param('sequencing_key').nil? && !@params.get_param('sequencing_key').empty?
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105
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has_full_key = !seq.seq_fasta.index(@params.get_param('sequencing_key')).nil?
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end
|
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data/lib/seqtrimnext.rb
CHANGED
metadata
CHANGED
@@ -2,7 +2,7 @@
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2
2
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name: seqtrimnext
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3
3
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version: !ruby/object:Gem::Version
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4
4
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prerelease:
|
5
|
-
version: 2.0.
|
5
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+
version: 2.0.38
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6
6
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platform: ruby
|
7
7
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authors:
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8
8
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- Dario Guerrero & Almudena Bocinos
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@@ -10,7 +10,7 @@ autorequire:
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10
10
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bindir: bin
|
11
11
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cert_chain: []
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12
12
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13
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-
date: 2011-
|
13
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+
date: 2011-07-07 00:00:00 Z
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14
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dependencies:
|
15
15
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- !ruby/object:Gem::Dependency
|
16
16
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name: narray
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