ruby-ensembl-api 0.9.6 → 1.0
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- data/TUTORIAL.rdoc +1 -1
- data/bin/variation_effect_predictor +106 -0
- data/lib/ensembl.rb +2 -2
- data/lib/ensembl/core/activerecord.rb +119 -225
- data/lib/ensembl/core/collection.rb +14 -10
- data/lib/ensembl/core/project.rb +6 -8
- data/lib/ensembl/core/slice.rb +87 -123
- data/lib/ensembl/core/transcript.rb +49 -65
- data/lib/ensembl/core/transform.rb +6 -8
- data/lib/ensembl/db_connection.rb +56 -72
- data/lib/ensembl/variation/activerecord.rb +138 -8
- data/lib/ensembl/variation/variation.rb +284 -46
- data/samples/ensembl_genomes_example.rb +60 -0
- data/samples/examples_perl_tutorial.rb +125 -0
- data/samples/small_example_ruby_api.rb +34 -0
- data/samples/variation_example.rb +67 -0
- data/test/unit/{release_56 → release_60}/core/test_gene.rb +6 -6
- data/test/unit/release_60/core/test_project_human.rb +38 -0
- data/test/unit/{release_56 → release_60}/core/test_slice.rb +1 -8
- data/test/unit/release_60/core/test_transcript.rb +126 -0
- data/test/unit/{release_53 → release_60}/core/test_transform.rb +21 -21
- data/test/unit/release_60/variation/test_activerecord.rb +213 -0
- data/test/unit/release_60/variation/test_consequence.rb +158 -0
- data/test/unit/{release_56 → release_60}/variation/test_variation.rb +18 -17
- data/test/unit/test_connection.rb +2 -2
- data/test/unit/test_releases.rb +8 -8
- metadata +27 -43
- data/test/unit/data/seq_c6qbl.fa +0 -10
- data/test/unit/data/seq_cso19_coding.fa +0 -16
- data/test/unit/data/seq_cso19_transcript.fa +0 -28
- data/test/unit/data/seq_drd3_gene.fa +0 -838
- data/test/unit/data/seq_drd3_transcript.fa +0 -22
- data/test/unit/data/seq_drd4_transcript.fa +0 -24
- data/test/unit/data/seq_forward_composite.fa +0 -1669
- data/test/unit/data/seq_par_boundary.fa +0 -169
- data/test/unit/data/seq_rnd3_transcript.fa +0 -47
- data/test/unit/data/seq_ub2r1_coding.fa +0 -13
- data/test/unit/data/seq_ub2r1_gene.fa +0 -174
- data/test/unit/data/seq_ub2r1_transcript.fa +0 -26
- data/test/unit/data/seq_y.fa +0 -2
- data/test/unit/ensembl_genomes/test_collection.rb +0 -51
- data/test/unit/ensembl_genomes/test_gene.rb +0 -52
- data/test/unit/ensembl_genomes/test_slice.rb +0 -71
- data/test/unit/ensembl_genomes/test_variation.rb +0 -17
- data/test/unit/release_50/core/test_project.rb +0 -215
- data/test/unit/release_50/core/test_project_human.rb +0 -58
- data/test/unit/release_50/core/test_relationships.rb +0 -66
- data/test/unit/release_50/core/test_sequence.rb +0 -175
- data/test/unit/release_50/core/test_slice.rb +0 -121
- data/test/unit/release_50/core/test_transcript.rb +0 -108
- data/test/unit/release_50/core/test_transform.rb +0 -223
- data/test/unit/release_50/variation/test_activerecord.rb +0 -143
- data/test/unit/release_50/variation/test_variation.rb +0 -84
- data/test/unit/release_53/core/test_gene.rb +0 -66
- data/test/unit/release_53/core/test_project.rb +0 -96
- data/test/unit/release_53/core/test_project_human.rb +0 -65
- data/test/unit/release_53/core/test_slice.rb +0 -47
- data/test/unit/release_53/variation/test_activerecord.rb +0 -145
- data/test/unit/release_53/variation/test_variation.rb +0 -71
- data/test/unit/release_56/core/test_project.rb +0 -96
- data/test/unit/release_56/core/test_transform.rb +0 -63
- data/test/unit/release_56/variation/test_activerecord.rb +0 -142
@@ -1,175 +0,0 @@
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#
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# = test/unit/test_seq.rb - Unit test for Ensembl::Core
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#
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# Copyright:: Copyright (C) 2007
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# Jan Aerts <http://jandot.myopenid.com>
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# License:: Ruby's
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#
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# $Id:
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require 'pathname'
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libpath = Pathname.new(File.join(File.dirname(__FILE__), ['..'] * 4, 'lib')).cleanpath.to_s
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$:.unshift(libpath) unless $:.include?(libpath)
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require 'test/unit'
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require 'yaml'
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require 'lib/ensembl'
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include Ensembl::Core
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DBConnection.connect('homo_sapiens', 50)
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#class SequenceForSlice < Test::Unit::TestCase
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# def test_forward_strand_seqlevel
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# slice = Slice.new(SeqRegion.find(170931),5,15)
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# seq = 'gcagtggtgtg'
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# assert_equal(seq, slice.seq)
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# end
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#
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# def test_reverse_strand_seqlevel
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# slice = Slice.new(SeqRegion.find(170931),5,15, -1)
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# seq = 'cacaccactgc'
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# assert_equal(seq, slice.seq)
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# end
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#
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# def test_forward_strand_not_seqlevel_single_target
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# slice = Slice.new(SeqRegion.find(226044),69437100,69437110)
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# seq = 'gtctatttaca'
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# assert_equal(seq, slice.seq)
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# end
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#
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# def test_reverse_strand_not_seqlevel_single_target
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# slice = Slice.new(SeqRegion.find(226044),69437100,69437110,-1)
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# seq = 'tgtaaatagac'
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# assert_equal(seq, slice.seq)
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# end
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#
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# def test_forward_strand_not_seqlevel_composite_target
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# seq = ''
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# File.open('../../data/seq_forward_composite.fa').reject{|l| l=~/^>/}.each do |line|
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# line.chomp!
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# seq += line
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# end
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# seq.downcase!
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# slice = Slice.new(SeqRegion.find(226044),69387650,69487649)
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# assert_equal(seq, slice.seq)
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# end
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#
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# def test_reverse_strand_not_seqlevel_composite_target
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# slice = Slice.new(SeqRegion.find(226044),69437061,69437160,-1)
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# assert_equal('nnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnatgtaaatagacaactaacaagatagctgagattgtttccttatccagaca', slice.seq)
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# end
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#
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#end
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#
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#class SequenceForUnsplicedFeature < Test::Unit::TestCase
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# def test_forward_strand_seqlevel
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# marker_feature = MarkerFeature.find(1323757)
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# marker_seq = 'ggcttacttggaaaggtctcttccaacccaatattattcaaatactttcaattttcttctaatgtttttagtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtggttttgttttatttcttttaattctctgatacatttagaatttcttttattattttattttattttattatttatttatttatttttgagacagagttttgctc'
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# assert_equal(marker_seq, marker_feature.seq)
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# end
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#
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# def test_reverse_strand_seqlevel
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# gene = Gene.find_by_name('ANKRD56')
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#
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# ankrd56_seq = 'atggcccgagagctgagccaggaggcactactggactttctgtgccaggctgggggccgcgtgaccaacgctgccttgctgagccacttcaagagctttctccgagaccccgacgcgtcccccagccagcaccagcaccgccgcgagctcttcaagggcttcgtcaactcggtcgccgcagtgcgccaggaccccgacggcaccaagtacgtggtgctcaagaggagatacagggaccttttgggggaggaggggctgcagcgaccccgcgagccgcccgcggccgcccccagtgcagggggagctgcgccctgctccccgcgaggcgcgcgccggggggagccgccccagcagcagcccaggcggcggcggcgcgagaaggagccggaggaggagccagcaggtgcagcagccagagccgccgacgcagcttgcaatggactcccgggcagcgactcccgtagggcgcccgggaagggcggcggatcgaagggcagtcccggacagaggccgccggtgcccgcagctgcagcggcaggggcccaggcgagagcgagctgcgcggcggcgaagacgcagggccgctgctgctgggaatgcctccagaacaacctggctgtactgccgggagagctcggcgcactcccgcactcggccaccgcggaggagaagccggcacgggctctgcctgcccaggatgaccgcggggcttccagggagcgggaagaaggcgcgctagctgagccggcgcctgtgcctgcagtggctcactcgcctcccgccaccgtcgaggctgcgacaagcagggcttccccgcctgctctcctgcccggccccgctccccgcggagaccggccggagctgctgacccccagctccctgcattattcgaccctgcagcagcagcagcagcgcactcgagagtgggtggccaggcacccgcaggtgcccgaggcccgtgatcagggccctatccgcgcctggtcggtgctgccagacaacttcctccagctgcccttggaacccggctccacggagcctaattcagagccgccagacccctgtctttcctcgcactctctctttcctgttgttccggatgagtcctgggaatcctgggcggggaacccttcattgactgtctttcgcagcattcgttgtcagctgtccctccaagatctggatgactttgtggaccaggagagtgatggcagtgaggagagcagcagtgggcccaaagactccccgggggcttctgaagaggggctgcaggttgtcttgggaaccccagatagggggaagctcaggaatccagctgggggcctttctgtatctcggaaggagggcagccccagccggagccctcagggtctcagaaacagaggggatggtcacatctctcagcaggtccctgcaggggctaatggccttgcaggccaccccctgaagcctttgccttggccagttcctaagttaaggaggtccctcaggaggagctctctggcagggagagccaaattgtcctcctctgatgaggagtacctcgatgagggcttgctgaaaagaagtcggcgcccacctcgatccaggaagccctccaaggcaggaacggcacccagcccaagggttgatgcaggtttatcactaaaacttgcagaggttaaggctgttgtggccgagcggggttggcgacacagcctgtgggtccccagtggggaggggtctgcagccttggccccccacagaacttctgagcacaaatcatccctggttccactagatgccagggagcatgagtggattgtgaagcttgccagtggctcctggattcaggtgtggactttgttctgggaggaccctcaactggccttgcacaaagactttttgactgggtacactgcgttgcactggatagccaaacatggtgacctcagggcccttcaggacttggtgtctggagcaaagaaggcagggattgtccttgatgtaaacgtgaggtccagttgtggatataccccgctgcaccttgcagccattcacggccaccagggggtcatcaaattgctagtgcaaaggttggcttctcgggtaaatgtcagggacagcagtgggaagaagccatggcagtatctaaccagtaatacctctggggaaatatggcagctgttgggagctcctcggggcaagcccattttccctgtctatcccttagttggaagttcttcccctaccagaaaggccaagagcaaggaaatatctagaagtgtcacccgaaaaacttccttcgctgcactactcaaaagtcagcacaacaagtggaaactggccaaccagtatgagaaattccacagtccaagggaaagagaagagtatagtgactga'
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# assert_equal(ankrd56_seq, gene.seq)
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# end
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#
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# def test_reverse_strand_not_seqlevel
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# gene = Gene.find_by_name('DRD3')
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# drd3_gene_seq = ''
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# File.open('../../data/seq_drd3_gene.fa').reject{|l| l=~/^>/}.each do |line|
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# line.chomp!
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# drd3_gene_seq += line
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# end
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# drd3_gene_seq.downcase!
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# assert_equal(drd3_gene_seq, gene.seq)
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#
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# end
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#
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# def test_exon
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# exon = Exon.find(719588)
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# assert_equal('atggcatctctgagccagctgagtggccacctgaactacacctgtggggcagagaactccacaggtgccagccaggcccgcccacatgcctactatgccctctcctactgcgcgctcatcctggccatcgtcttcggcaatggcctggtgtgcatggctgtgctgaaggagcgggccctgcagactaccaccaactacttagtagtgagcctggctgtggcagacttgctggtggccaccttggtgatgccctgggtggtatacctggag', exon.seq)
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# end
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#
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#end
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class SequenceForSlicedFeature < Test::Unit::TestCase
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def test_transcript_foward
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transcript = Transcript.find(73491) # UB2R1 = CDC34
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ub2r1_transcript_seq = ''
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File.open('test/unit/data/seq_ub2r1_transcript.fa').reject{|l| l=~/^>/}.each do |line|
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line.chomp!
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ub2r1_transcript_seq += line
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end
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ub2r1_transcript_seq.downcase!
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assert_equal(ub2r1_transcript_seq, transcript.seq)
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end
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def test_transcript_reverse
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transcript = Transcript.find(107548)
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rnd3_transcript_seq = ''
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File.open('test/unit/data/seq_rnd3_transcript.fa').reject{|l| l=~/^>/}.each do |line|
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line.chomp!
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rnd3_transcript_seq += line
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end
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rnd3_transcript_seq.downcase!
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assert_equal(rnd3_transcript_seq, transcript.seq)
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end
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end
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class SequenceForCDS < Test::Unit::TestCase
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def setup
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# Transcript tr_fw is ENST00000215574
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@tr_fw = Transcript.find(73491)
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# Transcript tr_rev is ENST00000315489
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@tr_rev = Transcript.find(73411)
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end
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def test_cds_fw
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ub2r1_coding_seq = ''
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File.open('test/unit/data/seq_ub2r1_coding.fa').reject{|l| l=~/^>/}.each do |line|
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line.chomp!
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ub2r1_coding_seq += line
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end
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ub2r1_coding_seq.downcase!
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assert_equal(ub2r1_coding_seq, @tr_fw.cds_seq)
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end
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def test_cds_rev
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cso19_coding_seq = ''
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File.open('test/unit/data/seq_cso19_coding.fa').reject{|l| l=~/^>/}.each do |line|
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line.chomp!
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cso19_coding_seq += line
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end
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cso19_coding_seq.downcase!
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assert_equal(cso19_coding_seq, @tr_rev.cds_seq)
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end
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def test_five_prime_utr_fw
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assert_equal('GGCAAGCGCCGGTGGGGCGGCGGCGCCAGAGCTGCTGGAGCGCTCGGGGTCCCCGGGCGGCGGCGGCGGCGCAGAGGAGGAGGCAGGCGGCGGCCCCGGTGGCTCCCCCCCGGACGGTGCGCGGCCCGGCCCGTCTCGCGAACTCGCGGTGGTCGCGCGGCCCCGCGCTGCTCCGACCCCGGGCCCCTCCGCCGCCGCC'.downcase, @tr_fw.five_prime_utr_seq)
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end
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def test_five_primer_utr_rev
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assert_equal('ACTCGATCCCGGCCCCACTTCCAGGCCAGTGTCCGGCCGACCAGCCTGCCTTGGGCCAGGGCCCCACGACTCCCTGCTGCGGGACAAGAGGCCGTCTGTGCGGCTGTGGTCGTGGGAGGGTGTGGTGAGGCCGTGAAGGTGGGGACGGTGCCTGGGCCTGTGGCCGCCAGAGCTGCTGCGGCTCAGAAGGTAGCACCAGGCCCCGTGGGTGCTGTGGGGGCCATCGCCTGCCCACC'.downcase, @tr_rev.five_prime_utr_seq)
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end
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def test_three_prime_utr_fw
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assert_equal('CACCACCAGAATAAACTTGCCGAGTTTACCTCACTAGGGCCGGACCCGTGGCTCCTTAGACGACAGACTACCTCACGGAGGTTTTGTGCTGGTCCCCGTCTCCTCTGGTTGTTTCGTTTTGGCTTTTTCTCCCTCCCCATGTCTGTTCTGGGTTTTCACGTGCTTCAGAGAAGAGGGGCTGCCCCACCGCCACTCACGTCACTCGGGGCTCGGTGGACGGGCCCAGGGTGGGAGCGGCCGGCCCACCTGTCCCCTCGGGAGGGGAGCTGAGCCCGACTTCTACCGGGGTCCCCCAGCTTCCGGACTGGCCGCACCCCGGAGGAGCCACGGGGGCGCTGCTGGGAACGTGGGCGGGGGGCCGTTTCCTGACACTACCAGCCTGGGAGGCCCAGGTGTAGCGGTCCGAGGGGCCCGGTCCTGCCTGTCAGCTCCAGGTCCTGGAGCCACGTCCAGCACAGAGTGGACGGATTCACCGTGGCCGACTCTTTTCCCTGCTTTGGTTTGTTTGAAATCTAAATAAAACTACTTTATG'.downcase, @tr_fw.three_prime_utr_seq)
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end
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def test_three_prime_utr_rev
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assert_equal('GCCAGGCCTGTTTCCCACACAGTGAACGGGGGGCTGGCGGGCCTCTCCTGGGGCCTGGCTTTGGCTGGAGGGGCTAGAAGGCGAGAGGGGCTGGGAAAGGAGTCTTCCCTCCCCTTTCCTGAACTGTCCAGGTCCTTCTAGGACACCTGATCCTTCCAGTCCCTGGGGGCTGTGACCCATGGCCCTGTGCAGGGTGCAGGGTGAGTCTCCTTCCAGTGCCCCAGTTTCTTCCAGGTCACGCCAGGCCAGGCCAGGCCAGGTGGGGAAAGGGACACCTTCCGGCCCTCCCCAGTAGCTGGCTGGAGATGGAGCTTCCTGTGTCCCAGAACTCGGCGTCCAGCTCCACTAGGGCCTGGATCCCCATCACAGCTTGGGTTAGCCCCGGTCCCAGCCCAAACTCAGGCTGGAGGCAGCCCCGAGGCCTGTGCCTTTCCCACTCCACCTTCTACAGTTGCTTAGCCAATAAACCTTTCCTGGGCTGGAG'.downcase, @tr_rev.three_prime_utr_seq)
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end
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def test_protein_fw
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assert_equal('MARPLVPSSQKALLLELKGLQEEPVEGFRVTLVDEGDLYNWEVAIFGPPNTYYEGGYFKARLKFPIDYPYSPPAFRFLTKMWHPNIYETGDVCISILHPPVDDPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPANVDASVMYRKWKESKGKDREYTDIIRKQVLGTKVDAERDGVKVPTTLAEYCVKTKAPAPDEGSDLFYDDYYEDGEVEEEADSCFGDDEDDSGTEES*', @tr_fw.protein_seq)
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end
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def test_protein_rev
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assert_equal('MGTLSCDSTPRLATAPLGRRVTEGQIPETGLRKSCGTATLENGSGPGLYVLPSTVGFINHDCTRVASPAYSLVRRPSEAPPQDTSPGPIYFLDPKVTRFGRSCTPAYSMQGRAKSRGPEVTPGPGAYSPEKVPPVRHRTPPAFTLGCRLPLKPLDTSAPAPNAYTMPPLWGSQIFTKPSSPSYTVVGRTPPARPPQDPAEIPGPGQYDSPDANTYRQRLPAFTMLGRPRAPRPLEETPGPGAHCPEQVTVNKARAPAFSMGIRHSKRASTMAATTPSRPAGHRLPGRCC*', @tr_rev.protein_seq)
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end
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end
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#
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# = test/unit/test_project.rb - Unit test for Ensembl::Core
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#
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# Copyright:: Copyright (C) 2007
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# Jan Aerts <http://jandot.myopenid.com>
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# License:: Ruby's
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#
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# $Id:
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require 'pathname'
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libpath = Pathname.new(File.join(File.dirname(__FILE__), ['..'] * 4, 'lib')).cleanpath.to_s
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$:.unshift(libpath) unless $:.include?(libpath)
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require 'test/unit'
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require 'lib/ensembl'
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|
16
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include Ensembl::Core
|
17
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DBConnection.connect('bos_taurus', 50)
|
18
|
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|
19
|
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class GetFeatures < Test::Unit::TestCase
|
20
|
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# Chr4.003.122 has no simple features in itself, but the corresponding region
|
21
|
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# covered by the chromosome has 37. In addition, contigs within the scaffold
|
22
|
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# have 85. Total should therefore be 122.
|
23
|
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def test_simple_features
|
24
|
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contig = SeqRegion.find_by_name('AAFC03055312')
|
25
|
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assert_equal(19, contig.simple_features.length)
|
26
|
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assert_equal(19, contig.slice.simple_features.length)
|
27
|
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slice = Slice.fetch_by_region('contig','AAFC03055312')
|
28
|
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assert_equal(19, slice.simple_features.length)
|
29
|
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end
|
30
|
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end
|
31
|
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|
32
|
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class SliceMethodMissing < Test::Unit::TestCase
|
33
|
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def setup
|
34
|
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@slice = Slice.fetch_by_region('chromosome','4',10000,10000000)
|
35
|
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end
|
36
|
-
|
37
|
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# There is not NotExistingTable class
|
38
|
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def test_non_existing_tables
|
39
|
-
assert_raise(NoMethodError) { @slice.not_existing_tables }
|
40
|
-
end
|
41
|
-
|
42
|
-
# A slice can get its exons
|
43
|
-
def test_exons
|
44
|
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assert_equal(291, @slice.exons.length)
|
45
|
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assert_equal(Exon, @slice.exons[0].class)
|
46
|
-
end
|
47
|
-
|
48
|
-
# A slice can _not_ get its markers; it has marker_features instead.
|
49
|
-
def test_markers
|
50
|
-
assert_raise(NoMethodError) { @slice.markers }
|
51
|
-
end
|
52
|
-
|
53
|
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def test_transcripts
|
54
|
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assert_equal(36, @slice.transcripts.length)
|
55
|
-
end
|
56
|
-
end
|
57
|
-
|
58
|
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class GetOverlappingObjects < Test::Unit::TestCase
|
59
|
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def setup
|
60
|
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@small_slice = Slice.fetch_by_region('chromosome','Un.004.10515',850,900)
|
61
|
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@genes_inclusive = @small_slice.genes(true)
|
62
|
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@genes_exclusive = @small_slice.genes
|
63
|
-
|
64
|
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@large_slice = Slice.fetch_by_region('contig','AAFC03055312',1,18210)
|
65
|
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@repeats_inclusive = @large_slice.repeat_features(true).select{|r| r.analysis_id == 6}
|
66
|
-
end
|
67
|
-
|
68
|
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def test_get_gene
|
69
|
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assert_equal(1, @genes_inclusive.length)
|
70
|
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assert_equal('ENSBTAG00000039669', @genes_inclusive[0].stable_id)
|
71
|
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assert_equal(0, @genes_exclusive.length)
|
72
|
-
end
|
73
|
-
|
74
|
-
def test_get_repeat_features
|
75
|
-
assert_equal(2, @repeats_inclusive.length)
|
76
|
-
end
|
77
|
-
end
|
78
|
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|
79
|
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class ExcisingSlice < Test::Unit::TestCase
|
80
|
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def setup
|
81
|
-
@original_slice = Slice.fetch_by_region('chromosome','1',1,1000)
|
82
|
-
end
|
83
|
-
|
84
|
-
def test_excise_one_range
|
85
|
-
output = @original_slice.excise([20..50])
|
86
|
-
assert_equal(2, output.length)
|
87
|
-
assert_equal('chromosome:Btau_4.0:1:1:19:1', output[0].to_s)
|
88
|
-
assert_equal('chromosome:Btau_4.0:1:51:1000:1', output[1].to_s)
|
89
|
-
end
|
90
|
-
|
91
|
-
def test_excise_two_nonoverlapping_ranges
|
92
|
-
output = @original_slice.excise([20..50,100..200])
|
93
|
-
assert_equal(3, output.length)
|
94
|
-
assert_equal('chromosome:Btau_4.0:1:1:19:1', output[0].to_s)
|
95
|
-
assert_equal('chromosome:Btau_4.0:1:51:99:1', output[1].to_s)
|
96
|
-
assert_equal('chromosome:Btau_4.0:1:201:1000:1', output[2].to_s)
|
97
|
-
end
|
98
|
-
|
99
|
-
def test_excise_two_overlapping_ranges
|
100
|
-
output = @original_slice.excise([20..150,100..200])
|
101
|
-
assert_equal(2, output.length)
|
102
|
-
assert_equal('chromosome:Btau_4.0:1:1:19:1', output[0].to_s)
|
103
|
-
assert_equal('chromosome:Btau_4.0:1:201:1000:1', output[1].to_s)
|
104
|
-
end
|
105
|
-
|
106
|
-
def test_excise_two_adjacent_ranges
|
107
|
-
output = @original_slice.excise([20..99,100..200])
|
108
|
-
assert_equal(2, output.length)
|
109
|
-
assert_equal('chromosome:Btau_4.0:1:1:19:1', output[0].to_s)
|
110
|
-
assert_equal('chromosome:Btau_4.0:1:201:1000:1', output[1].to_s)
|
111
|
-
end
|
112
|
-
|
113
|
-
def test_excise_internal_ranges
|
114
|
-
output = @original_slice.excise([20..300,100..200])
|
115
|
-
assert_equal(2, output.length)
|
116
|
-
assert_equal('chromosome:Btau_4.0:1:1:19:1', output[0].to_s)
|
117
|
-
assert_equal('chromosome:Btau_4.0:1:201:1000:1', output[1].to_s)
|
118
|
-
end
|
119
|
-
|
120
|
-
|
121
|
-
end
|
@@ -1,108 +0,0 @@
|
|
1
|
-
#
|
2
|
-
# = test/unit/test_transcript.rb - Unit test for Ensembl::Core
|
3
|
-
#
|
4
|
-
# Copyright:: Copyright (C) 2007
|
5
|
-
# Jan Aerts <http://jandot.myopenid.com>
|
6
|
-
# License:: Ruby's
|
7
|
-
#
|
8
|
-
# $Id:
|
9
|
-
require 'pathname'
|
10
|
-
libpath = Pathname.new(File.join(File.dirname(__FILE__), ['..'] * 4, 'lib')).cleanpath.to_s
|
11
|
-
$:.unshift(libpath) unless $:.include?(libpath)
|
12
|
-
|
13
|
-
require 'test/unit'
|
14
|
-
|
15
|
-
require 'lib/ensembl'
|
16
|
-
|
17
|
-
include Ensembl::Core
|
18
|
-
|
19
|
-
DBConnection.connect('homo_sapiens', 50)
|
20
|
-
|
21
|
-
class CodingPositions < Test::Unit::TestCase
|
22
|
-
def setup
|
23
|
-
# Transcript tr_fw is ENST00000215574
|
24
|
-
@tr_fw = Transcript.find(73491)
|
25
|
-
# Transcript tr_rev is ENST00000358041
|
26
|
-
@tr_rev = Transcript.find(73774)
|
27
|
-
end
|
28
|
-
|
29
|
-
def test_transcript_coords
|
30
|
-
assert_equal(482733, @tr_fw.seq_region_start)
|
31
|
-
assert_equal(493084, @tr_fw.seq_region_end)
|
32
|
-
assert_equal(595371, @tr_rev.seq_region_start)
|
33
|
-
assert_equal(598309, @tr_rev.seq_region_end)
|
34
|
-
end
|
35
|
-
|
36
|
-
def test_coding_regions_genomic_coords_of_fw
|
37
|
-
assert_equal(482932, @tr_fw.coding_region_genomic_start)
|
38
|
-
assert_equal(492552, @tr_fw.coding_region_genomic_end)
|
39
|
-
end
|
40
|
-
|
41
|
-
def test_coding_regions_genomic_coords_of_rev
|
42
|
-
assert_equal(597652, @tr_rev.coding_region_genomic_start)
|
43
|
-
assert_equal(598047, @tr_rev.coding_region_genomic_end)
|
44
|
-
end
|
45
|
-
|
46
|
-
def test_coding_regions_cdna_coords_of_fw
|
47
|
-
assert_equal(200, @tr_fw.coding_region_cdna_start)
|
48
|
-
assert_equal(910, @tr_fw.coding_region_cdna_end)
|
49
|
-
end
|
50
|
-
|
51
|
-
def test_coding_regions_cdna_coords_of_rev
|
52
|
-
assert_equal(263, @tr_rev.coding_region_cdna_start)
|
53
|
-
assert_equal(658, @tr_rev.coding_region_cdna_end)
|
54
|
-
end
|
55
|
-
|
56
|
-
end
|
57
|
-
|
58
|
-
class GenomicVsCDna < Test::Unit::TestCase
|
59
|
-
def setup
|
60
|
-
# Transcript tr_fw is ENST00000215574
|
61
|
-
@tr_fw = Transcript.find(73491)
|
62
|
-
# Transcript tr_rev is ENST00000315489
|
63
|
-
@tr_rev = Transcript.find(73411)
|
64
|
-
end
|
65
|
-
|
66
|
-
def test_identify_exon
|
67
|
-
assert_equal(Exon.find(374767), @tr_fw.exon_for_cdna_position(601))
|
68
|
-
assert_equal(Exon.find(374767), @tr_fw.exon_for_genomic_position(488053))
|
69
|
-
assert_equal(Exon.find(374458), @tr_rev.exon_for_cdna_position(541))
|
70
|
-
assert_equal(Exon.find(374458), @tr_rev.exon_for_genomic_position(418719))
|
71
|
-
end
|
72
|
-
|
73
|
-
def test_cdna2genomic
|
74
|
-
assert_equal(488053, @tr_fw.cdna2genomic(601))
|
75
|
-
assert_equal(418719, @tr_rev.cdna2genomic(541))
|
76
|
-
end
|
77
|
-
|
78
|
-
def test_cds2genomic
|
79
|
-
assert_equal(488053, @tr_fw.cds2genomic(401))
|
80
|
-
assert_equal(418719, @tr_rev.cds2genomic(304))
|
81
|
-
end
|
82
|
-
|
83
|
-
def test_genomic2cdna
|
84
|
-
assert_equal(601, @tr_fw.genomic2cdna(488053))
|
85
|
-
assert_equal(541, @tr_rev.genomic2cdna(418719))
|
86
|
-
end
|
87
|
-
|
88
|
-
def test_genomic2cds
|
89
|
-
assert_equal(401, @tr_fw.genomic2cds(488053))
|
90
|
-
assert_equal(304, @tr_rev.genomic2cds(418719))
|
91
|
-
end
|
92
|
-
end
|
93
|
-
|
94
|
-
class TestIntron < Test::Unit::TestCase
|
95
|
-
def setup
|
96
|
-
@transcript = Transcript.find(58973)
|
97
|
-
@introns = @transcript.introns
|
98
|
-
end
|
99
|
-
|
100
|
-
def test_get_introns
|
101
|
-
assert_equal(2, @introns.length)
|
102
|
-
end
|
103
|
-
|
104
|
-
def test_intron_slices
|
105
|
-
assert_equal('chromosome:NCBI36:8:159418:172128:-1', @introns[0].slice.to_s)
|
106
|
-
end
|
107
|
-
end
|
108
|
-
|
@@ -1,223 +0,0 @@
|
|
1
|
-
#
|
2
|
-
# = test/unit/test_transform.rb - Unit test for Ensembl::Core
|
3
|
-
#
|
4
|
-
# Copyright:: Copyright (C) 2007
|
5
|
-
# Jan Aerts <http://jandot.myopenid.com>
|
6
|
-
# License:: Ruby's
|
7
|
-
#
|
8
|
-
# $Id:
|
9
|
-
require 'pathname'
|
10
|
-
libpath = Pathname.new(File.join(File.dirname(__FILE__), ['..'] * 4, 'lib')).cleanpath.to_s
|
11
|
-
$:.unshift(libpath) unless $:.include?(libpath)
|
12
|
-
|
13
|
-
require 'test/unit'
|
14
|
-
|
15
|
-
require 'lib/ensembl'
|
16
|
-
|
17
|
-
include Ensembl::Core
|
18
|
-
DBConnection.connect('bos_taurus', 50)
|
19
|
-
|
20
|
-
# For all tests, the source (i.e. the seq_region that the feature is annotated
|
21
|
-
# on initially) remains forward.
|
22
|
-
#
|
23
|
-
# Same coordinate system: test names refer to direction of gene vs chromosome
|
24
|
-
class TransformOntoSameCoordinateSystem < Test::Unit::TestCase
|
25
|
-
# |-------|========>-------------------------> chromosome
|
26
|
-
# ^ ^
|
27
|
-
# | |
|
28
|
-
# |-------|========>-------------------------> chromosome
|
29
|
-
# This should return itself.
|
30
|
-
def test_fw
|
31
|
-
source_gene = Gene.find(6043)
|
32
|
-
target_gene = source_gene.transform('chromosome')
|
33
|
-
|
34
|
-
assert_equal('4', source_gene.seq_region.name)
|
35
|
-
assert_equal(4595538, source_gene.seq_region_start)
|
36
|
-
assert_equal(4827723, source_gene.seq_region_end)
|
37
|
-
assert_equal(1, source_gene.seq_region_strand)
|
38
|
-
assert_equal('4', target_gene.seq_region.name)
|
39
|
-
assert_equal(4595538, target_gene.seq_region_start)
|
40
|
-
assert_equal(4827723, target_gene.seq_region_end)
|
41
|
-
assert_equal(1, target_gene.seq_region_strand)
|
42
|
-
end
|
43
|
-
|
44
|
-
# |-------<========|-------------------------> chromosome
|
45
|
-
# ^ ^
|
46
|
-
# | |
|
47
|
-
# |-------<========|-------------------------> chromosome
|
48
|
-
# This should return itself.
|
49
|
-
def test_rev
|
50
|
-
source_gene = Gene.find(6053)
|
51
|
-
target_gene = source_gene.transform('chromosome')
|
52
|
-
|
53
|
-
assert_equal('4', source_gene.seq_region.name)
|
54
|
-
assert_equal(5199677, source_gene.seq_region_start)
|
55
|
-
assert_equal(5201728, source_gene.seq_region_end)
|
56
|
-
assert_equal(-1, source_gene.seq_region_strand)
|
57
|
-
assert_equal('4', target_gene.seq_region.name)
|
58
|
-
assert_equal(5199677, target_gene.seq_region_start)
|
59
|
-
assert_equal(5201728, target_gene.seq_region_end)
|
60
|
-
assert_equal(-1, target_gene.seq_region_strand)
|
61
|
-
end
|
62
|
-
end
|
63
|
-
|
64
|
-
## Test names refer to:
|
65
|
-
## (1) direction of gene vs chromosome
|
66
|
-
## (2) direction of component (scaffold) vs assembly (chromosome)
|
67
|
-
#class TransformFromComponentToAssembly < Test::Unit::TestCase
|
68
|
-
# def test_fw_fw
|
69
|
-
# assert true
|
70
|
-
# end
|
71
|
-
#
|
72
|
-
# def test_fw_rev
|
73
|
-
# assert true
|
74
|
-
# end
|
75
|
-
#
|
76
|
-
# def test_rev_fw
|
77
|
-
# assert true
|
78
|
-
# end
|
79
|
-
#
|
80
|
-
# def test_rev_rev
|
81
|
-
# assert true
|
82
|
-
# end
|
83
|
-
#end
|
84
|
-
#
|
85
|
-
## Test names refer to:
|
86
|
-
## (1) direction of gene vs chromosome
|
87
|
-
## (2) direction of component (scaffold) vs assembly (chromosome)
|
88
|
-
## We have to test for features that are covered by a scaffold, and those
|
89
|
-
## overlapping more than 1 scaffold.
|
90
|
-
#class TransformFromAssemblyToComponent < Test::Unit::TestCase
|
91
|
-
# # |-----------------> scaffold
|
92
|
-
# # ^ ^
|
93
|
-
# # | |
|
94
|
-
# # |---------|=====>-----------------------------> chromosome
|
95
|
-
# def test_fw_fw_full_overlap
|
96
|
-
# source_gene = Gene.find(2995)
|
97
|
-
# target_gene = source_gene.transform('scaffold')
|
98
|
-
#
|
99
|
-
# assert_equal('4', source_gene.seq_region.name)
|
100
|
-
# assert_equal(10333321, source_gene.seq_region_start)
|
101
|
-
# assert_equal(10510842, source_gene.seq_region_end)
|
102
|
-
# assert_equal(1, source_gene.seq_region_strand)
|
103
|
-
# assert_equal('Chr4.003.12', target_gene.seq_region.name)
|
104
|
-
# assert_equal(43842, target_gene.seq_region_start)
|
105
|
-
# assert_equal(221363, target_gene.seq_region_end)
|
106
|
-
# assert_equal(1, target_gene.seq_region_strand)
|
107
|
-
# end
|
108
|
-
#
|
109
|
-
# # |-----------------> scaffold
|
110
|
-
# # |
|
111
|
-
# # |
|
112
|
-
# # |---|===>-------------------------------> chromosome
|
113
|
-
# def test_fw_fw_partial_overlap
|
114
|
-
# source_feature = PredictionTranscript.find(52425)
|
115
|
-
# target_feature = source_feature.transform('scaffold')
|
116
|
-
#
|
117
|
-
# assert_equal('4', source_feature.seq_region.name)
|
118
|
-
# assert_equal(1443280, source_feature.seq_region_start)
|
119
|
-
# assert_equal(1482777, source_feature.seq_region_end)
|
120
|
-
# assert_equal(1, source_feature.seq_region_strand)
|
121
|
-
# assert_equal(nil, target_feature)
|
122
|
-
# end
|
123
|
-
#
|
124
|
-
# # <-----------------| scaffold
|
125
|
-
# # ^ ^
|
126
|
-
# # | |
|
127
|
-
# # |----------|=====>-----------------------------> chromosome
|
128
|
-
# def test_fw_rev_full_overlap
|
129
|
-
# source_gene = Gene.find(2708)
|
130
|
-
# target_gene = source_gene.transform('scaffold')
|
131
|
-
#
|
132
|
-
# assert_equal('4', source_gene.seq_region.name)
|
133
|
-
# assert_equal(8312492, source_gene.seq_region_start)
|
134
|
-
# assert_equal(8312812, source_gene.seq_region_end)
|
135
|
-
# assert_equal(1, source_gene.seq_region_strand)
|
136
|
-
# assert_equal('Chr4.003.10', target_gene.seq_region.name)
|
137
|
-
# assert_equal(1774466, target_gene.seq_region_start)
|
138
|
-
# assert_equal(1774786, target_gene.seq_region_end)
|
139
|
-
# assert_equal(-1, target_gene.seq_region_strand)
|
140
|
-
# end
|
141
|
-
#
|
142
|
-
# # <-----------------| scaffold
|
143
|
-
# # |
|
144
|
-
# # |
|
145
|
-
# # |---------------------|===>------------------> chromosome
|
146
|
-
# def test_fw_rev_partial_overlap
|
147
|
-
# source_feature = PredictionTranscript.find(23305)
|
148
|
-
# target_feature = source_feature.transform('scaffold')
|
149
|
-
#
|
150
|
-
# assert_equal('4', source_feature.seq_region.name)
|
151
|
-
# assert_equal(10008188, source_feature.seq_region_start)
|
152
|
-
# assert_equal(10156104, source_feature.seq_region_end)
|
153
|
-
# assert_equal(1, source_feature.seq_region_strand)
|
154
|
-
# assert_equal(nil, target_feature)
|
155
|
-
# end
|
156
|
-
#
|
157
|
-
# # |-----------------> scaffold
|
158
|
-
# # | ^ ^
|
159
|
-
# # | | |
|
160
|
-
# # |---<===|--<=====|-----------------------------> chromosome
|
161
|
-
# def test_rev_fw_full_overlap
|
162
|
-
# source_gene = Gene.find(3124)
|
163
|
-
# target_gene = source_gene.transform('scaffold')
|
164
|
-
#
|
165
|
-
# assert_equal('4', source_gene.seq_region.name)
|
166
|
-
# assert_equal(10353230, source_gene.seq_region_start)
|
167
|
-
# assert_equal(10371155, source_gene.seq_region_end)
|
168
|
-
# assert_equal(-1, source_gene.seq_region_strand)
|
169
|
-
# assert_equal('Chr4.003.12', target_gene.seq_region.name)
|
170
|
-
# assert_equal(63751, target_gene.seq_region_start)
|
171
|
-
# assert_equal(81676, target_gene.seq_region_end)
|
172
|
-
# assert_equal(-1, target_gene.seq_region_strand)
|
173
|
-
# end
|
174
|
-
#
|
175
|
-
# # |-----------------> scaffold
|
176
|
-
# # |
|
177
|
-
# # |
|
178
|
-
# # |---------------------<===|------------------> chromosome
|
179
|
-
# def test_rev_fw_partial_overlap
|
180
|
-
# source_feature = PredictionTranscript.find(24185)
|
181
|
-
# target_feature = source_feature.transform('scaffold')
|
182
|
-
#
|
183
|
-
# assert_equal('4', source_feature.seq_region.name)
|
184
|
-
# assert_equal(11389212, source_feature.seq_region_start)
|
185
|
-
# assert_equal(11471635, source_feature.seq_region_end)
|
186
|
-
# assert_equal(-1, source_feature.seq_region_strand)
|
187
|
-
# assert_equal(nil, target_feature)
|
188
|
-
# end
|
189
|
-
#
|
190
|
-
# # <-----------------| scaffold
|
191
|
-
# # | ^ ^
|
192
|
-
# # | | |
|
193
|
-
# # |---<===|--<=====|-----------------------------> chromosome
|
194
|
-
# def test_rev_rev_full_overlap
|
195
|
-
# source_gene = Gene.find(2408)
|
196
|
-
# target_gene = source_gene.transform('scaffold')
|
197
|
-
#
|
198
|
-
# assert_equal('4', source_gene.seq_region.name)
|
199
|
-
# assert_equal(8104409, source_gene.seq_region_start)
|
200
|
-
# assert_equal(8496477, source_gene.seq_region_end)
|
201
|
-
# assert_equal(-1, source_gene.seq_region_strand)
|
202
|
-
# assert_equal('Chr4.003.10', target_gene.seq_region.name)
|
203
|
-
# assert_equal(1590801, target_gene.seq_region_start)
|
204
|
-
# assert_equal(1982869, target_gene.seq_region_end)
|
205
|
-
# assert_equal(1, target_gene.seq_region_strand)
|
206
|
-
# end
|
207
|
-
#
|
208
|
-
# # <-----------------| scaffold
|
209
|
-
# # |
|
210
|
-
# # |
|
211
|
-
# # |---<===|-----------------------------> chromosome
|
212
|
-
# def test_rev_rev_partial_overlap
|
213
|
-
# source_feature = Transcript.find(14723)
|
214
|
-
# target_feature = source_feature.transform('scaffold')
|
215
|
-
#
|
216
|
-
# assert_equal('4', source_feature.seq_region.name)
|
217
|
-
# assert_equal(55713316, source_feature.seq_region_start)
|
218
|
-
# assert_equal(55792273, source_feature.seq_region_end)
|
219
|
-
# assert_equal(-1, source_feature.seq_region_strand)
|
220
|
-
# assert_equal(nil, target_feature)
|
221
|
-
# end
|
222
|
-
#
|
223
|
-
#end
|