parse_fasta 0.0.5 → 1.0.0

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+ File: README
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+ </div>
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+
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+ <iframe id="search_frame"></iframe>
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+
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+ <div id="content"><div id='filecontents'>
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+ <h1 id="label-parse_fasta">parse_fasta</h1>
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+
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+ <p>So you want to parse a fasta file…</p>
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+
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+ <h2 id="label-Installation">Installation</h2>
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+
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+ <p>Add this line to your application&#39;s Gemfile:</p>
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+
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+ <pre class="code ruby"><code class="ruby"><span class='id identifier rubyid_gem'>gem</span> <span class='tstring'><span class='tstring_beg'>&#39;</span><span class='tstring_content'>parse_fasta</span><span class='tstring_end'>&#39;</span></span></code></pre>
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+
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+ <p>And then execute:</p>
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+
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+ <pre class="code ruby"><code class="ruby">$ bundle</code></pre>
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+
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+ <p>Or install it yourself as:</p>
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+
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+ <pre class="code ruby"><code class="ruby">$ gem install parse_fasta</code></pre>
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+
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+ <h2 id="label-Overview">Overview</h2>
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+
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+ <p>Provides the method <code>#each_record</code> for the <code>File</code>
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+ class.</p>
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+
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+ <pre class="code ruby"><code class="ruby"><span class='id identifier rubyid_each_record'>each_record</span> <span class='lbrace'>{</span> <span class='op'>|</span><span class='id identifier rubyid_header'>header</span><span class='comma'>,</span> <span class='id identifier rubyid_sequence'>sequence</span><span class='op'>|</span> <span class='id identifier rubyid_block'>block</span> <span class='rbrace'>}</span>
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+ </code></pre>
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+
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+ <p>The whole file is not loaded into memory, so have no fear of giant fasta
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+ files!</p>
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+
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+ <h2 id="label-Usage">Usage</h2>
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+
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+ <h3 id="label-Version+1.0.0+%28current%29">Version 1.0.0 (current)</h3>
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+
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+ <p>The monkey patch of the <code>File</code> class is no more! Here is the new
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+ print length example:</p>
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+
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+ <pre class="code ruby"><code class="ruby"><span class='id identifier rubyid_require'>require</span> <span class='tstring'><span class='tstring_beg'>&#39;</span><span class='tstring_content'>parse_fasta</span><span class='tstring_end'>&#39;</span></span>
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+
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+ <span class='const'>FastaFile</span><span class='period'>.</span><span class='id identifier rubyid_open'>open</span><span class='lparen'>(</span><span class='const'>ARGV</span><span class='period'>.</span><span class='id identifier rubyid_first'>first</span><span class='comma'>,</span> <span class='tstring'><span class='tstring_beg'>&#39;</span><span class='tstring_content'>r</span><span class='tstring_end'>&#39;</span></span><span class='rparen'>)</span><span class='period'>.</span><span class='id identifier rubyid_each_record'>each_record</span> <span class='kw'>do</span> <span class='op'>|</span><span class='id identifier rubyid_header'>header</span><span class='comma'>,</span> <span class='id identifier rubyid_sequence'>sequence</span><span class='op'>|</span>
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+ <span class='id identifier rubyid_puts'>puts</span> <span class='lbracket'>[</span><span class='id identifier rubyid_header'>header</span><span class='comma'>,</span> <span class='id identifier rubyid_sequence'>sequence</span><span class='period'>.</span><span class='id identifier rubyid_length'>length</span><span class='rbracket'>]</span><span class='period'>.</span><span class='id identifier rubyid_join'>join</span><span class='lparen'>(</span><span class='tstring'><span class='tstring_beg'>&quot;</span><span class='tstring_content'>\t</span><span class='tstring_end'>&quot;</span></span><span class='rparen'>)</span>
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+ <span class='kw'>end</span>
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+ </code></pre>
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+
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+ <p>And here, a script to calculate GC content:</p>
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+
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+ <pre class="code ruby"><code class="ruby"><span class='id identifier rubyid_require'>require</span> <span class='tstring'><span class='tstring_beg'>&#39;</span><span class='tstring_content'>parse_fasta</span><span class='tstring_end'>&#39;</span></span>
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+
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+ <span class='const'>FastaFile</span><span class='period'>.</span><span class='id identifier rubyid_open'>open</span><span class='lparen'>(</span><span class='const'>ARGV</span><span class='period'>.</span><span class='id identifier rubyid_first'>first</span><span class='comma'>,</span> <span class='tstring'><span class='tstring_beg'>&#39;</span><span class='tstring_content'>r</span><span class='tstring_end'>&#39;</span></span><span class='rparen'>)</span><span class='period'>.</span><span class='id identifier rubyid_each_record'>each_record</span> <span class='kw'>do</span> <span class='op'>|</span><span class='id identifier rubyid_header'>header</span><span class='comma'>,</span> <span class='id identifier rubyid_sequence'>sequence</span><span class='op'>|</span>
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+ <span class='id identifier rubyid_puts'>puts</span> <span class='lbracket'>[</span><span class='id identifier rubyid_header'>header</span><span class='comma'>,</span> <span class='id identifier rubyid_sequence'>sequence</span><span class='period'>.</span><span class='id identifier rubyid_gc'>gc</span><span class='rbracket'>]</span><span class='period'>.</span><span class='id identifier rubyid_join'>join</span><span class='lparen'>(</span><span class='tstring'><span class='tstring_beg'>&quot;</span><span class='tstring_content'>\t</span><span class='tstring_end'>&quot;</span></span><span class='rparen'>)</span>
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+ <span class='kw'>end</span>
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+ </code></pre>
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+
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+ <h3 id="label-Version+0.0.5+%28old%29">Version 0.0.5 (old)</h3>
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+
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+ <p>An example that lists the length for each sequence. (Won&#39;t work in
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+ version 1.0.0)</p>
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+
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+ <pre class="code ruby"><code class="ruby"><span class='id identifier rubyid_require'>require</span> <span class='tstring'><span class='tstring_beg'>&#39;</span><span class='tstring_content'>parse_fasta</span><span class='tstring_end'>&#39;</span></span>
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+
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+ <span class='const'>File</span><span class='period'>.</span><span class='id identifier rubyid_open'>open</span><span class='lparen'>(</span><span class='const'>ARGV</span><span class='period'>.</span><span class='id identifier rubyid_first'>first</span><span class='comma'>,</span> <span class='tstring'><span class='tstring_beg'>&#39;</span><span class='tstring_content'>r</span><span class='tstring_end'>&#39;</span></span><span class='rparen'>)</span><span class='period'>.</span><span class='id identifier rubyid_each_record'>each_record</span> <span class='kw'>do</span> <span class='op'>|</span><span class='id identifier rubyid_header'>header</span><span class='comma'>,</span> <span class='id identifier rubyid_sequence'>sequence</span><span class='op'>|</span>
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+ <span class='id identifier rubyid_puts'>puts</span> <span class='lbracket'>[</span><span class='id identifier rubyid_header'>header</span><span class='comma'>,</span> <span class='id identifier rubyid_sequence'>sequence</span><span class='period'>.</span><span class='id identifier rubyid_length'>length</span><span class='rbracket'>]</span><span class='period'>.</span><span class='id identifier rubyid_join'>join</span><span class='lparen'>(</span><span class='tstring'><span class='tstring_beg'>&quot;</span><span class='tstring_content'>\t</span><span class='tstring_end'>&quot;</span></span><span class='rparen'>)</span>
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+ <span class='kw'>end</span>
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+ </code></pre>
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+
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+ <h2 id="label-Benchmark">Benchmark</h2>
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+
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+ <p>Just for fun, I wanted to compare the execution time to that of BioRuby. I
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+ calculated sequence length for each fasta record with both the
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+ <code>each_record</code> method from this gem and using the
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+ <code>FastaFormat</code> class from BioRuby. You can see the test script in
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+ <code>benchmark.rb</code>.</p>
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+
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+ <p>The test file contained 2,009,897 illumina reads and the file size was 1.1
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+ gigabytes. Here are the results from Ruby&#39;s <code>Benchmark</code>
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+ class:</p>
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+
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+ <pre class="code ruby"><code class="ruby"> user system total real
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+ parse_fasta 64.530000 1.740000 66.270000 ( 67.081502)
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+ bioruby 116.250000 2.260000 118.510000 (120.223710)</code></pre>
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+
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+ <p>I just wanted a nice, clean way to parse fasta files, but being nearly
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+ twice as fasta as BioRuby doesn&#39;t hurt either!</p>
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+
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+ <h2 id="label-Notes">Notes</h2>
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+
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+ <p>Currently in doesn&#39;t check whether your file is actually a fasta file
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+ or anything, so watch out.</p>
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+ </div></div>
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+
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+ <div id="footer">
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+ Generated on Tue Jun 3 22:55:41 2014 by
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+ <a href="http://yardoc.org" title="Yay! A Ruby Documentation Tool" target="_parent">yard</a>
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+ 0.8.7.4 (ruby-2.1.1).
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+ </div>
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+
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+ </body>
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+ </html>
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+ $('#toc .hide_toc').toggle(function() {
192
+ $('#toc .top').slideUp('fast');
193
+ $('#toc').toggleClass('hidden');
194
+ $('#toc .title small').toggle();
195
+ }, function() {
196
+ $('#toc .top').slideDown('fast');
197
+ $('#toc').toggleClass('hidden');
198
+ $('#toc .title small').toggle();
199
+ });
200
+ $('#toc .float_toc').toggle(function() {
201
+ $(this).text('float');
202
+ $('#toc').toggleClass('nofloat');
203
+ }, function() {
204
+ $(this).text('left');
205
+ $('#toc').toggleClass('nofloat');
206
+ });
207
+ }
208
+
209
+ $(framesInit);
210
+ $(createSourceLinks);
211
+ $(createDefineLinks);
212
+ $(createFullTreeLinks);
213
+ $(fixBoxInfoHeights);
214
+ $(searchFrameLinks);
215
+ $(linkSummaries);
216
+ $(keyboardShortcuts);
217
+ $(summaryToggle);
218
+ $(fixOutsideWorldLinks);
219
+ $(generateTOC);
@@ -0,0 +1,178 @@
1
+ var inSearch = null;
2
+ var searchIndex = 0;
3
+ var searchCache = [];
4
+ var searchString = '';
5
+ var regexSearchString = '';
6
+ var caseSensitiveMatch = false;
7
+ var ignoreKeyCodeMin = 8;
8
+ var ignoreKeyCodeMax = 46;
9
+ var commandKey = 91;
10
+
11
+ RegExp.escape = function(text) {
12
+ return text.replace(/[-[\]{}()*+?.,\\^$|#\s]/g, "\\$&");
13
+ }
14
+
15
+ function fullListSearch() {
16
+ // generate cache
17
+ searchCache = [];
18
+ $('#full_list li').each(function() {
19
+ var link = $(this).find('.object_link a');
20
+ if (link.length === 0) return;
21
+ var fullName = link.attr('title').split(' ')[0];
22
+ searchCache.push({name:link.text(), fullName:fullName, node:$(this), link:link});
23
+ });
24
+
25
+ $('#search input').keyup(function(event) {
26
+ if ((event.keyCode > ignoreKeyCodeMin && event.keyCode < ignoreKeyCodeMax)
27
+ || event.keyCode == commandKey)
28
+ return;
29
+ searchString = this.value;
30
+ caseSensitiveMatch = searchString.match(/[A-Z]/) != null;
31
+ regexSearchString = RegExp.escape(searchString);
32
+ if (caseSensitiveMatch) {
33
+ regexSearchString += "|" +
34
+ $.map(searchString.split(''), function(e) { return RegExp.escape(e); }).
35
+ join('.+?');
36
+ }
37
+ if (searchString === "") {
38
+ clearTimeout(inSearch);
39
+ inSearch = null;
40
+ $('ul .search_uncollapsed').removeClass('search_uncollapsed');
41
+ $('#full_list, #content').removeClass('insearch');
42
+ $('#full_list li').removeClass('found').each(function() {
43
+
44
+ var link = $(this).find('.object_link a');
45
+ if (link.length > 0) link.text(link.text());
46
+ });
47
+ if (clicked) {
48
+ clicked.parents('ul').each(function() {
49
+ $(this).removeClass('collapsed').prev().removeClass('collapsed');
50
+ });
51
+ }
52
+ highlight();
53
+ }
54
+ else {
55
+ if (inSearch) clearTimeout(inSearch);
56
+ searchIndex = 0;
57
+ lastRowClass = '';
58
+ $('#full_list, #content').addClass('insearch');
59
+ $('#noresults').text('');
60
+ searchItem();
61
+ }
62
+ });
63
+
64
+ $('#search input').focus();
65
+ $('#full_list').after("<div id='noresults'></div>");
66
+ }
67
+
68
+ var lastRowClass = '';
69
+ function searchItem() {
70
+ for (var i = 0; i < searchCache.length / 50; i++) {
71
+ var item = searchCache[searchIndex];
72
+ var searchName = (searchString.indexOf('::') != -1 ? item.fullName : item.name);
73
+ var matchString = regexSearchString;
74
+ var matchRegexp = new RegExp(matchString, caseSensitiveMatch ? "" : "i");
75
+ if (searchName.match(matchRegexp) == null) {
76
+ item.node.removeClass('found');
77
+ }
78
+ else {
79
+ item.node.css('padding-left', '10px').addClass('found');
80
+ item.node.parents().addClass('search_uncollapsed');
81
+ item.node.removeClass(lastRowClass).addClass(lastRowClass == 'r1' ? 'r2' : 'r1');
82
+ lastRowClass = item.node.hasClass('r1') ? 'r1' : 'r2';
83
+ item.link.html(item.name.replace(matchRegexp, "<strong>$&</strong>"));
84
+ }
85
+
86
+ if (searchCache.length === searchIndex + 1) {
87
+ searchDone();
88
+ return;
89
+ }
90
+ else {
91
+ searchIndex++;
92
+ }
93
+ }
94
+ inSearch = setTimeout('searchItem()', 0);
95
+ }
96
+
97
+ function searchDone() {
98
+ highlight(true);
99
+ if ($('#full_list li:visible').size() === 0) {
100
+ $('#noresults').text('No results were found.').hide().fadeIn();
101
+ }
102
+ else {
103
+ $('#noresults').text('');
104
+ }
105
+ $('#content').removeClass('insearch');
106
+ clearTimeout(inSearch);
107
+ inSearch = null;
108
+ }
109
+
110
+ clicked = null;
111
+ function linkList() {
112
+ $('#full_list li, #full_list li a:last').click(function(evt) {
113
+ if ($(this).hasClass('toggle')) return true;
114
+ if (this.tagName.toLowerCase() == "li") {
115
+ if ($(this).find('.object_link a').length === 0) {
116
+ $(this).children('a.toggle').click();
117
+ return false;
118
+ }
119
+ var toggle = $(this).children('a.toggle');
120
+ if (toggle.size() > 0 && evt.pageX < toggle.offset().left) {
121
+ toggle.click();
122
+ return false;
123
+ }
124
+ }
125
+ if (clicked) clicked.removeClass('clicked');
126
+ var win = window.top.frames.main ? window.top.frames.main : window.parent;
127
+ if (this.tagName.toLowerCase() == "a") {
128
+ clicked = $(this).parents('li').addClass('clicked');
129
+ win.location = this.href;
130
+ }
131
+ else {
132
+ clicked = $(this).addClass('clicked');
133
+ win.location = $(this).find('a:last').attr('href');
134
+ }
135
+ return false;
136
+ });
137
+ }
138
+
139
+ function collapse() {
140
+ if (!$('#full_list').hasClass('class')) return;
141
+ $('#full_list.class a.toggle').click(function() {
142
+ $(this).parent().toggleClass('collapsed').next().toggleClass('collapsed');
143
+ highlight();
144
+ return false;
145
+ });
146
+ $('#full_list.class ul').each(function() {
147
+ $(this).addClass('collapsed').prev().addClass('collapsed');
148
+ });
149
+ $('#full_list.class').children().removeClass('collapsed');
150
+ highlight();
151
+ }
152
+
153
+ function highlight(no_padding) {
154
+ var n = 1;
155
+ $('#full_list li:visible').each(function() {
156
+ var next = n == 1 ? 2 : 1;
157
+ $(this).removeClass("r" + next).addClass("r" + n);
158
+ if (!no_padding && $('#full_list').hasClass('class')) {
159
+ $(this).css('padding-left', (10 + $(this).parents('ul').size() * 15) + 'px');
160
+ }
161
+ n = next;
162
+ });
163
+ }
164
+
165
+ function escapeShortcut() {
166
+ $(document).keydown(function(evt) {
167
+ if (evt.which == 27) {
168
+ $('#search_frame', window.top.document).slideUp(100);
169
+ $('#search a', window.top.document).removeClass('active inactive');
170
+ $(window.top).focus();
171
+ }
172
+ });
173
+ }
174
+
175
+ $(escapeShortcut);
176
+ $(fullListSearch);
177
+ $(linkList);
178
+ $(collapse);