mspire 0.2.4 → 0.3.0

data/test_files/bioworks_small.toppred.out

@@ -0,0 +1,2847 @@
+Algorithm specific parameters: 
+
+Full window size : 21
+Core window size : 11
+Wedge window size: 5
+Using hydrophobicity file: GES-scale
+
+Cutoff for certain transmembrane segments: 1.00
+Cutoff for putative transmembrane segments: 0.60
+Critical distance between 2 transmembrane segments: 2
+
+Critical loop length:  60
+
+Kingdom: procaryote
+
+Using cyt/ext file: CYTEXT-scale
+
+
+Sequence : gi_49175991_ref_YP_025292_1_  (50 res)
+MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVFTAYESE
+
+Found: 1 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1     5 - 25    2.260 Certain
+
+Total of 1 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  1.00        -1.00     0.00      1.00    -1.00
+TOPOLOGY                                  N-out        ?      N-in
+EXT_LOOP      1      4      4              2.00  (  0.06)
+TRANSMEM      5     25     21 1.00 2.26
+CYT_LOOP     26     50     25              3.00  ( -0.31)
+//
+
+Sequence : gi_16128018_ref_NP_414565_1_  (72 res)
+MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICAMAMKSHFFLISVLNRRLTLT
+AVQGILGRFSLF
+
+Found: 1 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1    33 - 53    1.332 Certain
+
+Total of 1 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  1.00         1.00     0.00     -2.00    -0.20
+TOPOLOGY                                   N-in        ?     N-out
+CYT_LOOP      1     32     32              3.00  ( -0.07)
+TRANSMEM     33     53     21 1.00 1.33
+EXT_LOOP     54     72     19              3.00  ( -0.24)
+//
+
+Sequence : gi_16128024_ref_NP_414571_1_  (304 res)
+MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEKTTRNALQLLHFWNAEI
+PLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIPAFLAIRDALMRAPEPVTL
+VAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTPNAEFNIAADPEAAACVFRSG
+IEIVMCGLDVTNQAILTPDYLSTLPQLNRTGKMLHALFSHYRSGSMQSGLRMHDLCAIAW
+LVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLGKPANVQVALDLDVKGFQQWVAEVL
+ALAS
+
+Found: 1 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1   117 - 137   1.361 Certain
+
+Total of 1 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  1.00         0.00    -0.09     -2.00     0.22
+TOPOLOGY                                      ?     N-in     N-out
+    LOOP      1    116    116           (  9.00)    0.07
+TRANSMEM    117    137     21 1.00 1.36
+    LOOP    138    304    167           ( 13.00)    0.16
+//
+
+Sequence : gi_16128058_ref_NP_414606_1_  (292 res)
+MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQ
+GREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFF
+RPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVR
+EACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLS
+TTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS
+
+Found: 1 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1     8 - 28    0.697 Putative
+
+Total of 2 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  0.24         1.00    -0.37      0.00     1.00
+TOPOLOGY                                   N-in     N-in         ?
+CYT_LOOP      1      7      7              0.00  ( -0.55)
+TRANSMEM      8     28     21 0.24 0.70
+EXT_LOOP     29    292    264           ( 29.00)    0.37
+//
+
+Sequence : gi_16128092_ref_NP_414641_1_  (129 res)
+MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGETPEQAVVRELQEEVGI
+TPQHFSLFEKLEYEFPDRHITLWFWLVERWEGEPWGKEGQPGEWMSLVGLNADDFPPANE
+PVIAKLKRL
+
+Found: 0 segments
+
+
+Sequence : gi_16128121_ref_NP_414670_1_  (256 res)
+MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGNLIGSRIGDMHGFSYMQ
+FIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPTHVIIAGYVGGGVARGLFV
+GILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLNGVFAKTFDDISLVPTFVLTP
+LTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGFLGINDVPLVTTFGVLVVFIVAF
+YLICWSLIQRGRGLRS
+
+Found: 7 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1    27 - 47    1.848 Certain
+     2    54 - 74    1.693 Certain
+     3    95 - 115   1.290 Certain
+     4   138 - 158   2.271 Certain
+     5   170 - 190   1.648 Certain
+     6   194 - 214   0.663 Putative
+     7   228 - 248   2.367 Certain
+
+Total of 2 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  1.00         8.00     0.00      2.00    -0.60
+TOPOLOGY                                   N-in        ?      N-in
+CYT_LOOP      1     26     26              4.00  (  0.36)
+TRANSMEM     27     47     21 1.00 1.85
+EXT_LOOP     48     53      6              1.00  ( -0.39)
+TRANSMEM     54     74     21 1.00 1.69
+CYT_LOOP     75     94     20              2.00  (  0.69)
+TRANSMEM     95    115     21 1.00 1.29
+EXT_LOOP    116    137     22              1.00  (  1.04)
+TRANSMEM    138    158     21 1.00 2.27
+CYT_LOOP    159    169     11              1.00  (  0.18)
+TRANSMEM    170    190     21 1.00 1.65
+EXT_LOOP    191    227     37              1.00  (  1.31)
+TRANSMEM    228    248     21 1.00 2.37
+CYT_LOOP    249    256      8              3.00  ( -0.52)
+//
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   2                  0.16         4.00     0.00      2.00    -0.60
+TOPOLOGY                                   N-in        ?      N-in
+CYT_LOOP      1     26     26              4.00  (  0.36)
+TRANSMEM     27     47     21 1.00 1.85
+EXT_LOOP     48     53      6              1.00  ( -0.39)
+TRANSMEM     54     74     21 1.00 1.69
+CYT_LOOP     75     94     20              2.00  (  0.69)
+TRANSMEM     95    115     21 1.00 1.29
+EXT_LOOP    116    137     22              1.00  (  1.04)
+TRANSMEM    138    158     21 1.00 2.27
+CYT_LOOP    159    169     11              1.00  (  0.18)
+TRANSMEM    170    190     21 1.00 1.65
+EXT_LOOP    191    193      3              0.00  ( -0.67)
+TRANSMEM    194    214     21 0.16 0.66
+CYT_LOOP    215    227     13              1.00  (  1.19)
+TRANSMEM    228    248     21 1.00 2.37
+EXT_LOOP    249    256      8              3.00  ( -0.52)
+//
+
+Sequence : gi_90111097_ref_NP_414731_2_  (84 res)
+MNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASDLVSRKNVEYLVVEAAGET
+RELRLDKITSFSHPEIGTVVVSES
+
+Found: 0 segments
+
+
+Sequence : gi_16128191_ref_NP_414740_1_  (217 res)
+MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPGQIIANAKLYRTVSAIV
+NIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAPFIARMVENALLEIPTGLI
+EASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGYSAMGGAVGAGGLGQIGYQYG
+YIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK
+
+Found: 4 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1    16 - 36    2.260 Certain
+     2    63 - 83    1.518 Certain
+     3   149 - 169   1.729 Certain
+     4   187 - 207   1.860 Certain
+
+Total of 1 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  1.00         2.00     0.40     -2.00     0.33
+TOPOLOGY                                   N-in    N-out     N-out
+CYT_LOOP      1     15     15              1.00  (  0.36)
+TRANSMEM     16     36     21 1.00 2.26
+EXT_LOOP     37     62     26              4.00  (  0.78)
+TRANSMEM     63     83     21 1.00 1.52
+CYT_LOOP     84    148     65           (  5.00)    0.40
+TRANSMEM    149    169     21 1.00 1.73
+EXT_LOOP    170    186     17              0.00  (  0.71)
+TRANSMEM    187    207     21 1.00 1.86
+CYT_LOOP    208    217     10              4.00  ( -0.29)
+//
+
+Sequence : gi_16128232_ref_NP_414781_1_  (158 res)
+MKQLSFLPGEMTPQDRRLIQRALRALDRHLHEPGVAFTSTHAVREWLRLHMAALEREEFR
+VLYLDNQNQLIAHETLFTGTINRTEVHPREVVKRALHFNAAAVILAHNHPSGETTPSQAD
+KTLTQRLVQVLQLVDIRVPDHLIVGGRQIYSFAEHGLL
+
+Found: 0 segments
+
+
+Sequence : gi_16128248_ref_NP_414797_1_  (120 res)
+MESLPGQIDKSLDEASLSLRAGSLRTITHILLPLLRPAILSALIYSFVRAITTVSAIVFL
+VTPDTRVATAYILNRVEDGEYGVAIAYGSILIVVMLAIIFIFDWLIGESRTSRSKAKNQA
+
+
+Found: 2 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1    43 - 63    1.197 Certain
+     2    82 - 102   2.268 Certain
+
+Total of 1 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  1.00         7.00     0.00      1.00     0.00
+TOPOLOGY                                   N-in        ?      N-in
+CYT_LOOP      1     42     42              4.00  ( -0.53)
+TRANSMEM     43     63     21 1.00 1.20
+EXT_LOOP     64     81     18              2.00  ( -0.06)
+TRANSMEM     82    102     21 1.00 2.27
+CYT_LOOP    103    120     18              4.00  ( -0.23)
+//
+
+Sequence : gi_90111117_ref_NP_414887_2_  (403 res)
+MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQAFALDKMQMGWIFSAGI
+LGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWDFPSLVFARLMTGVGLGAA
+LPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGFAGANLAWQTVFWVGGVVPLI
+LVPLLMRWLPESAVFAGEKQSAPPLRALFAPETATATLLLWLCYFFTLLVVYMLINWLPL
+LLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDKLRPVTMSLLIYSGMLASLLALGTV
+SSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSSQIRATGVGTAVAVGRLGAMSGPLLAG
+KMLALGTGTVGVMAASAPGILVAGLAVFILMSRRSRIQPCADA
+
+Found: 11 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1     9 - 29    1.440 Certain
+     2    54 - 74    1.915 Certain
+     3    82 - 102   1.992 Certain
+     4   111 - 131   1.206 Certain
+     5   143 - 163   1.739 Certain
+     6   166 - 186   1.916 Certain
+     7   215 - 235   2.249 Certain
+     8   254 - 274   1.696 Certain
+     9   280 - 300   1.913 Certain
+    10   340 - 360   0.835 Putative
+    11   372 - 392   2.014 Certain
+
+Total of 2 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  1.00        15.00    -1.02      4.00    -1.00
+TOPOLOGY                                   N-in     N-in      N-in
+CYT_LOOP      1      8      8              2.00  (  0.34)
+TRANSMEM      9     29     21 1.00 1.44
+EXT_LOOP     30     53     24              1.00  ( -0.39)
+TRANSMEM     54     74     21 1.00 1.91
+CYT_LOOP     75     81      7              4.00  ( -0.65)
+TRANSMEM     82    102     21 1.00 1.99
+EXT_LOOP    103    110      8              1.00  (  0.78)
+TRANSMEM    111    131     21 1.00 1.21
+CYT_LOOP    132    142     11              2.00  (  0.65)
+TRANSMEM    143    163     21 1.00 1.74
+EXT_LOOP    164    165      2              0.00  (  0.05)
+TRANSMEM    166    186     21 1.00 1.92
+CYT_LOOP    187    214     28              3.00  (  0.19)
+TRANSMEM    215    235     21 1.00 2.25
+EXT_LOOP    236    253     18              0.00  (  0.46)
+TRANSMEM    254    274     21 1.00 1.70
+CYT_LOOP    275    279      5              2.00  ( -0.87)
+TRANSMEM    280    300     21 1.00 1.91
+EXT_LOOP    301    371     71           (  3.00)    1.02
+TRANSMEM    372    392     21 1.00 2.01
+CYT_LOOP    393    403     11              3.00  ( -1.31)
+//
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   2                  0.59         9.00     0.00      4.00    -1.00
+TOPOLOGY                                   N-in        ?      N-in
+CYT_LOOP      1      8      8              2.00  (  0.34)
+TRANSMEM      9     29     21 1.00 1.44
+EXT_LOOP     30     53     24              1.00  ( -0.39)
+TRANSMEM     54     74     21 1.00 1.91
+CYT_LOOP     75     81      7              4.00  ( -0.65)
+TRANSMEM     82    102     21 1.00 1.99
+EXT_LOOP    103    110      8              1.00  (  0.78)
+TRANSMEM    111    131     21 1.00 1.21
+CYT_LOOP    132    142     11              2.00  (  0.65)
+TRANSMEM    143    163     21 1.00 1.74
+EXT_LOOP    164    165      2              0.00  (  0.05)
+TRANSMEM    166    186     21 1.00 1.92
+CYT_LOOP    187    214     28              3.00  (  0.19)
+TRANSMEM    215    235     21 1.00 2.25
+EXT_LOOP    236    253     18              0.00  (  0.46)
+TRANSMEM    254    274     21 1.00 1.70
+CYT_LOOP    275    279      5              2.00  ( -0.87)
+TRANSMEM    280    300     21 1.00 1.91
+EXT_LOOP    301    339     39              1.00  (  1.01)
+TRANSMEM    340    360     21 0.59 0.84
+CYT_LOOP    361    371     11              1.00  (  0.21)
+TRANSMEM    372    392     21 1.00 2.01
+EXT_LOOP    393    403     11              3.00  ( -1.31)
+//
+
+Sequence : gi_16128374_ref_NP_414923_1_  (63 res)
+MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEHPTAQEAMDAKKRYEDP
+DKE
+
+Found: 0 segments
+
+
+Sequence : gi_16128403_ref_NP_414952_1_  (172 res)
+MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLAAIPFWYLMTFLPWQLY
+SLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITLMALPTNDWQWVAAGFVIF
+RILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILYFIGHHWPLGILS
+
+Found: 3 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1    37 - 57    1.857 Certain
+     2    87 - 107   0.906 Putative
+     3   148 - 168   1.341 Certain
+
+Total of 2 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  0.77         9.00     0.00      0.00    -0.67
+TOPOLOGY                                   N-in        ?         ?
+CYT_LOOP      1     36     36              5.00  (  1.15)
+TRANSMEM     37     57     21 1.00 1.86
+EXT_LOOP     58     86     29              1.00  (  0.42)
+TRANSMEM     87    107     21 0.77 0.91
+CYT_LOOP    108    147     40              4.00  (  0.34)
+TRANSMEM    148    168     21 1.00 1.34
+EXT_LOOP    169    172      4              0.00  (  0.26)
+//
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   2                  1.00         6.00    -0.42      0.00    -0.67
+TOPOLOGY                                   N-in     N-in         ?
+CYT_LOOP      1     36     36              5.00  (  1.15)
+TRANSMEM     37     57     21 1.00 1.86
+EXT_LOOP     58    147     90           (  5.00)    0.42
+TRANSMEM    148    168     21 1.00 1.34
+CYT_LOOP    169    172      4              0.00  (  0.26)
+//
+
+Sequence : gi_16128443_ref_NP_414992_1_  (183 res)
+MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHTLRQQILADLFGQVTEA
+YIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCMLAPGVHIYTATHPIDPVAR
+NSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGAVVTKDVPDNVVVGGNPARII
+KKL
+
+Found: 0 segments
+
+
+Sequence : gi_16128499_ref_NP_415048_1_  (261 res)
+MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLVKNIIPGFENCDATILSTPKLGASFV
+DYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFALSEGGYLYCPPGSLMTFV
+NAQAEDSQIFLYKRRYVPVEGYAPWLVSGNASELERIHYEGMDDVILLDFLPKELGFDMN
+MHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNNWIPVKKGDYIFMGAYSLQAGYG
+VGRGEAFSYIYSKDCNRDVEI
+
+Found: 1 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1   104 - 124   0.814 Putative
+
+Total of 2 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  0.53         0.00    -0.53     -1.00     0.12
+TOPOLOGY                                      ?     N-in     N-out
+    LOOP      1    103    103           (  7.00)    0.62
+TRANSMEM    104    124     21 0.53 0.81
+    LOOP    125    261    137           ( 10.00)    1.15
+//
+
+Sequence : gi_16128537_ref_NP_415086_1_  (71 res)
+MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGSLVFGLLTYLTNLYFKI
+KEDKRKAARGE
+
+Found: 2 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1     7 - 27    1.103 Certain
+     2    34 - 54    1.875 Certain
+
+Total of 1 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  1.00         7.00     0.00      1.00    -0.33
+TOPOLOGY                                   N-in        ?      N-in
+CYT_LOOP      1      6      6              2.00  ( -0.07)
+TRANSMEM      7     27     21 1.00 1.10
+EXT_LOOP     28     33      6              1.00  (  0.27)
+TRANSMEM     34     54     21 1.00 1.87
+CYT_LOOP     55     71     17              6.00  ( -0.65)
+//
+
+Sequence : gi_49176035_ref_YP_025295_1_  (50 res)
+MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVLAYEPKK
+
+Found: 1 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1     5 - 25    2.295 Certain
+
+Total of 1 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  1.00        -4.00     0.00      3.00    -1.00
+TOPOLOGY                                  N-out        ?      N-in
+EXT_LOOP      1      4      4              1.00  ( -0.42)
+TRANSMEM      5     25     21 1.00 2.29
+CYT_LOOP     26     50     25              5.00  ( -0.21)
+//
+
+Sequence : gi_16128614_ref_NP_415164_1_  (87 res)
+MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYTPTVKPSSKGNYHSVSI
+TINATHIEQVETLYEELGKIDIVRMVL
+
+Found: 0 segments
+
+
+Sequence : gi_16128621_ref_NP_415171_1_  (203 res)
+MRLWLIRHGETQANIDGLYSGHAPTPLTARGIEQAQNLHTLLHGVSFDLVLCSELERAQH
+TARLVLSDRQLPVQIIPELNEMFFGDWEMRHHRDLMQEDAENYSAWCNDWQHAIPTNGEG
+FQAFSQRVERFIARLSEFQHYQNILVVSHQGVLSLLIARLIGMPAEAMWHFRVDQGCWSA
+IDINQKFATLRVLNSRAIGVENA
+
+Found: 1 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1   142 - 162   0.677 Putative
+
+Total of 2 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  0.19        -5.00    -0.32      1.00     0.27
+TOPOLOGY                                  N-out     N-in      N-in
+EXT_LOOP      1    141    141           ( 11.00)   -0.32
+TRANSMEM    142    162     21 0.19 0.68
+CYT_LOOP    163    203     41              5.00  (  0.17)
+//
+
+Sequence : gi_16128710_ref_NP_415263_1_  (97 res)
+MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTSELEIWHGLLLMWAVCA
+GVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF
+
+Found: 3 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1    17 - 37    1.689 Certain
+     2    48 - 68    1.779 Certain
+     3    77 - 97    1.810 Certain
+
+Total of 1 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  1.00         5.00     0.00      4.00    -0.60
+TOPOLOGY                                   N-in        ?      N-in
+CYT_LOOP      1     16     16              4.00  (  0.15)
+TRANSMEM     17     37     21 1.00 1.69
+EXT_LOOP     38     47     10              2.00  ( -0.74)
+TRANSMEM     48     68     21 1.00 1.78
+CYT_LOOP     69     76      8              2.00  (  0.34)
+TRANSMEM     77     97     21 1.00 1.81
+//
+
+Sequence : gi_90111171_ref_NP_415327_2_  (86 res)
+MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATGASNLSDLEDKLAEKAR
+EQGAKGYVINSAGGNDQMFGTATIYK
+
+Found: 1 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1     3 - 23    1.778 Certain
+
+Total of 1 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  1.00         2.00    -0.46      2.00    -1.00
+TOPOLOGY                                   N-in     N-in      N-in
+CYT_LOOP      1      2      2              2.00  ( -0.06)
+TRANSMEM      3     23     21 1.00 1.78
+EXT_LOOP     24     86     63           (  6.00)    0.46
+//
+
+Sequence : gi_16128811_ref_NP_415364_1_  (94 res)
+MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSVPQAVSVATVPQTVYVA
+PAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH
+
+Found: 2 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1     5 - 25    1.916 Certain
+     2    46 - 66    0.882 Putative
+
+Total of 2 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  0.71         2.00     0.00      1.00    -1.00
+TOPOLOGY                                   N-in        ?      N-in
+CYT_LOOP      1      4      4              1.00  ( -0.13)
+TRANSMEM      5     25     21 1.00 1.92
+EXT_LOOP     26     45     20              1.00  (  1.86)
+TRANSMEM     46     66     21 0.71 0.88
+CYT_LOOP     67     94     28              2.00  (  0.58)
+//
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   2                  1.00         1.00    -1.74      1.00    -1.00
+TOPOLOGY                                   N-in     N-in      N-in
+CYT_LOOP      1      4      4              1.00  ( -0.13)
+TRANSMEM      5     25     21 1.00 1.92
+EXT_LOOP     26     94     69           (  3.00)    1.74
+//
+
+Sequence : gi_16128871_ref_NP_415424_1_  (285 res)
+MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVFISIAFVFYITATTGTG
+TMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKASGRITWGQLAKNWLNVYFG
+NLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHTFIEAVCLGILANLMVCLAVW
+MSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIPMGIVIRDFASPEFWTAVGSAPE
+NFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWVIYLRENDHH
+
+Found: 6 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1    34 - 54    2.194 Certain
+     2    68 - 88    1.985 Certain
+     3   117 - 137   1.896 Certain
+     4   164 - 184   1.834 Certain
+     5   191 - 211   1.559 Certain
+     6   259 - 279   1.551 Certain
+
+Total of 1 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  1.00         8.00     0.00      0.00    -0.11
+TOPOLOGY                                   N-in        ?         ?
+CYT_LOOP      1     33     33              5.00  (  0.24)
+TRANSMEM     34     54     21 1.00 2.19
+EXT_LOOP     55     67     13              1.00  (  0.01)
+TRANSMEM     68     88     21 1.00 1.99
+CYT_LOOP     89    116     28              3.00  (  0.76)
+TRANSMEM    117    137     21 1.00 1.90
+EXT_LOOP    138    163     26              1.00  (  0.60)
+TRANSMEM    164    184     21 1.00 1.83
+CYT_LOOP    185    190      6              2.00  ( -0.46)
+TRANSMEM    191    211     21 1.00 1.56
+EXT_LOOP    212    258     47              1.00  (  0.87)
+TRANSMEM    259    279     21 1.00 1.55
+CYT_LOOP    280    285      6              1.00  ( -0.26)
+//
+
+Sequence : gi_90111191_ref_NP_415463_2_  (171 res)
+MLKRIIWILFLLGLTWGCELFAHDGTVNISGSFRRNTCVLAQDSKQINVQLGDVSLTRFS
+HGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSIESGTDAASGLAIAILDDA
+KILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKVNASATFVLHYD
+
+Found: 1 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1     3 - 23    1.353 Certain
+
+Total of 1 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  1.00         2.00    -1.08      2.00    -1.00
+TOPOLOGY                                   N-in     N-in      N-in
+CYT_LOOP      1      2      2              2.00  ( -0.26)
+TRANSMEM      3     23     21 1.00 1.35
+EXT_LOOP     24    171    148           ( 10.00)    1.08
+//
+
+Sequence : gi_90111193_ref_NP_415466_4_  (180 res)
+MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAFSPAGFCVDDAALYFSF
+EEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHGEMWVPMPGDAACVWLSDT
+HEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKIMNDRLKPQVNVDSFSLEQAV
+
+
+Found: 1 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1   132 - 152   0.774 Putative
+
+Total of 2 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  0.43        -4.00     0.24     -6.00     0.14
+TOPOLOGY                                  N-out    N-out     N-out
+EXT_LOOP      1    131    131           ( 15.00)    0.24
+TRANSMEM    132    152     21 0.43 0.77
+CYT_LOOP    153    180     28              4.00  (  0.44)
+//
+
+Sequence : gi_16128957_ref_NP_415511_1_  (76 res)
+MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEMAYNISYLTRVPGTNTL
+LACCCLLRPEEVNSEY
+
+Found: 1 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1    13 - 33    1.657 Certain
+
+Total of 1 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  1.00         2.00     0.00      7.00    -1.00
+TOPOLOGY                                   N-in        ?      N-in
+CYT_LOOP      1     12     12              4.00  ( -0.30)
+TRANSMEM     13     33     21 1.00 1.66
+EXT_LOOP     34     76     43              2.00  ( -0.32)
+//
+
+Sequence : gi_16128974_ref_NP_415528_1_  (196 res)
+MNEAVSPGALSTLFTDARTHNGWRETPVSDETLREIYALMKWGPTSANCSPARIVFTRTA
+EGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFPHGDARSWFTSSPQLAEET
+AFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFTGSTLKSNLLINIGYGDSSKL
+YARLPRLSFEEACGLL
+
+Found: 0 segments
+
+
+Sequence : gi_16129015_ref_NP_415570_1_  (32 res)
+MEQSTSSSNTPGIELIFRLSYLQKRRISYPFP
+
+Found: 0 segments
+
+
+Sequence : gi_16129146_ref_NP_415701_1_  (139 res)
+MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLLIQHPSATYFVKASGDS
+MIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLRPTVQLIPMNSAYSPITIS
+SEDTLDVFGVVIHVVKAMR
+
+Found: 1 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1    11 - 31    0.718 Putative
+
+Total of 2 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  0.29         2.00    -0.91      2.00    -0.33
+TOPOLOGY                                   N-in     N-in      N-in
+CYT_LOOP      1     10     10              2.00  ( -0.72)
+TRANSMEM     11     31     21 0.29 0.72
+EXT_LOOP     32    139    108           (  7.00)    0.91
+//
+
+Sequence : gi_16129250_ref_NP_415805_1_  (117 res)
+MIDKIKSNARDLRRNLTLQERKLWRYLRSRRFSDFKFRRQHPVGSYILDFACCSARVVVE
+LDGGQHDLAVAYDSRRTSWLESQGWTVLRFWNNEIDCNEETVLENILQELNRRSPSP
+
+Found: 0 segments
+
+
+Sequence : gi_16129266_ref_NP_415821_1_  (74 res)
+MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLAQLADEAKRMRERIQAL
+ESILDAEHPNWRDR
+
+Found: 1 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1     1 - 21    2.438 Certain
+
+Total of 1 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  1.00        -9.00     0.00     -1.00     0.00
+TOPOLOGY                                  N-out        ?     N-out
+TRANSMEM      1     21     21 1.00 2.44
+CYT_LOOP     22     74     53              9.00  ( -0.79)
+//
+
+Sequence : gi_16129267_ref_NP_415822_1_  (119 res)
+MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLSIFFGLALFTLVAYIIL
+SFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREMERYVTSDTFTLRSRFRQL
+
+Found: 1 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1    39 - 59    2.391 Certain
+
+Total of 1 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  1.00         0.00     0.00      3.00    -0.71
+TOPOLOGY                                   N-in        ?      N-in
+    LOOP      1     38     38              6.00  (  0.94)
+TRANSMEM     39     59     21 1.00 2.39
+    LOOP     60    119     60              7.00  ( -0.70)
+//
+
+Sequence : gi_16129318_ref_NP_415875_1_  (98 res)
+MKKENYSFKQACAVVGGQSAMARLLGVSPPSVNQWIKGVRQLPAERCPAIERATRGEVLC
+EELRPDIDWSYLRRSACCSQNMSVKQLNDSNKSSFDHT
+
+Found: 0 segments
+
+
+Sequence : gi_90111262_ref_NP_415894_4_  (144 res)
+MNRTILVPIDISDSELTQRVISHVEEEAKIDDAEVHFLTVIPSLPYYASLGLAYSAELPA
+MDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILELAKKIPAHMIIIASHRPDI
+TTYLLGSNAAAVVRHAECSVLVVR
+
+Found: 1 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1    35 - 55    1.239 Certain
+
+Total of 1 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  1.00         4.00    -0.01     -3.00     0.50
+TOPOLOGY                                   N-in     N-in     N-out
+CYT_LOOP      1     34     34              3.00  ( -0.52)
+TRANSMEM     35     55     21 1.00 1.24
+EXT_LOOP     56    144     89           ( 13.00)    0.01
+//
+
+Sequence : gi_16129357_ref_NP_415914_1_  (140 res)
+MSHKAWQNAHAMYENDACAKALGIDIISMDEGFAVVTMTVTAQMLNGHQSCHGGQLFSLA
+DTAFAYACNSQGLAAVASACTIDFLRPGFAGDTLTATAQVRHQGKQTGVYDIEIVNQQQK
+TVALFRGKSHRIGGTITGEA
+
+Found: 2 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1    31 - 51    0.779 Putative
+     2    62 - 82    0.816 Putative
+
+Total of 4 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  0.45        10.00     0.00     -2.00     0.33
+TOPOLOGY                                   N-in        ?     N-out
+CYT_LOOP      1     30     30              2.00  ( -0.24)
+TRANSMEM     31     51     21 0.45 0.78
+EXT_LOOP     52     61     10              0.00  (  0.13)
+TRANSMEM     62     82     21 0.54 0.82
+CYT_LOOP     83    140     58              7.00  (  0.03)
+//
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   2                  0.54        -7.00     0.13      0.00     0.50
+TOPOLOGY                                  N-out    N-out         ?
+EXT_LOOP      1     61     61           (  2.00)    0.13
+TRANSMEM     62     82     21 0.54 0.82
+CYT_LOOP     83    140     58              7.00  (  0.03)
+//
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   3                  0.45         3.00    -0.04     -2.00     0.33
+TOPOLOGY                                   N-in     N-in     N-out
+CYT_LOOP      1     30     30              2.00  ( -0.24)
+TRANSMEM     31     51     21 0.45 0.78
+EXT_LOOP     52    140     89           (  7.00)    0.04
+//
+
+Sequence : gi_90111267_ref_NP_415926_4_  (201 res)
+MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLLTGLLLMLAAQPILFLLLPI
+VLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALYLPFLFLPESNASLVILML
+FCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGLAVAIYPQWMQWNNLLWSIAS
+ILLLWTAINRCRSVLLMSAEI
+
+Found: 3 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1    31 - 51    2.129 Certain
+     2   113 - 133   1.851 Certain
+     3   169 - 189   1.329 Certain
+
+Total of 1 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  1.00         5.00     0.50      0.00    -1.00
+TOPOLOGY                                   N-in    N-out         ?
+CYT_LOOP      1     30     30              3.00  (  0.51)
+TRANSMEM     31     51     21 1.00 2.13
+EXT_LOOP     52    112     61           (  3.00)   -0.50
+TRANSMEM    113    133     21 1.00 1.85
+CYT_LOOP    134    168     35              3.00  (  1.02)
+TRANSMEM    169    189     21 1.00 1.33
+EXT_LOOP    190    201     12              2.00  ( -0.69)
+//
+
+Sequence : gi_16129388_ref_NP_415946_1_  (330 res)
+MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGLVILAMIIWGLLTSAFI
+ARLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFRPLAVCLFSFGVVVQLAYA
+AWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALGYTDAGLVFLGAGVFSWLSLE
+PVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSVNGGEGDTLAKMLFGYGLLQLLF
+MLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGSGSDNGFFHTLAVPLFIFTNFIIAI
+LLIRTFALLMQGKLLVRTERAVLMKAEDKE
+
+Found: 9 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1     7 - 27    1.717 Certain
+     2    41 - 61    2.072 Certain
+     3    81 - 101   2.083 Certain
+     4   141 - 161   1.039 Certain
+     5   164 - 184   1.503 Certain
+     6   199 - 219   1.360 Certain
+     7   225 - 245   1.097 Certain
+     8   255 - 275   1.686 Certain
+     9   283 - 303   1.979 Certain
+
+Total of 1 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  1.00        -7.00     0.00     -1.00     0.00
+TOPOLOGY                                  N-out        ?     N-out
+EXT_LOOP      1      6      6              1.00  (  0.49)
+TRANSMEM      7     27     21 1.00 1.72
+CYT_LOOP     28     40     13              1.00  (  1.00)
+TRANSMEM     41     61     21 1.00 2.07
+EXT_LOOP     62     80     19              3.00  (  0.75)
+TRANSMEM     81    101     21 1.00 2.08
+CYT_LOOP    102    140     39              2.00  (  0.80)
+TRANSMEM    141    161     21 1.00 1.04
+EXT_LOOP    162    163      2              0.00  ( -0.81)
+TRANSMEM    164    184     21 1.00 1.50
+CYT_LOOP    185    198     14              3.00  ( -0.70)
+TRANSMEM    199    219     21 1.00 1.36
+EXT_LOOP    220    224      5              1.00  ( -0.23)
+TRANSMEM    225    245     21 1.00 1.10
+CYT_LOOP    246    254      9              1.00  (  1.09)
+TRANSMEM    255    275     21 1.00 1.69
+EXT_LOOP    276    282      7              0.00  (  0.79)
+TRANSMEM    283    303     21 1.00 1.98
+CYT_LOOP    304    330     27              6.00  ( -0.96)
+//
+
+Sequence : gi_16129463_ref_NP_416021_1_  (176 res)
+MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDSLNFTVDLQKNSARQFP
+TTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLKLDEGSNTASGLGIEILDA
+NMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSVNPGLVRASATFTLEFQ
+
+Found: 2 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1     3 - 23    1.535 Certain
+     2    58 - 78    0.807 Putative
+
+Total of 2 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  0.52        -3.00     0.67      1.00    -1.00
+TOPOLOGY                                  N-out    N-out      N-in
+EXT_LOOP      1      2      2              1.00  ( -0.06)
+TRANSMEM      3     23     21 1.00 1.54
+CYT_LOOP     24     57     34              4.00  (  0.71)
+TRANSMEM     58     78     21 0.52 0.81
+EXT_LOOP     79    176     98           (  9.00)    0.67
+//
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   2                  1.00         1.00    -0.83      1.00    -1.00
+TOPOLOGY                                   N-in     N-in      N-in
+CYT_LOOP      1      2      2              1.00  ( -0.06)
+TRANSMEM      3     23     21 1.00 1.54
+EXT_LOOP     24    176    153           ( 13.00)    0.83
+//
+
+Sequence : gi_16129488_ref_NP_416046_1_  (221 res)
+MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQSLMASVYVFAIMMVAYY
+AGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSPEAKSKSEELEDEPSANIA
+FVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAPPLIFFLVAVILWGSLRSSGA
+IMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIKTYH
+
+Found: 6 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1    12 - 32    1.555 Certain
+     2    43 - 63    1.776 Certain
+     3    69 - 89    1.143 Certain
+     4   121 - 141   1.602 Certain
+     5   154 - 174   2.309 Certain
+     6   194 - 214   1.593 Certain
+
+Total of 1 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  1.00        -7.00     0.00     -1.00     0.00
+TOPOLOGY                                  N-out        ?     N-out
+EXT_LOOP      1     11     11              1.00  ( -0.47)
+TRANSMEM     12     32     21 1.00 1.56
+CYT_LOOP     33     42     10              2.00  (  0.00)
+TRANSMEM     43     63     21 1.00 1.78
+EXT_LOOP     64     68      5              0.00  ( -0.07)
+TRANSMEM     69     89     21 1.00 1.14
+CYT_LOOP     90    120     31              4.00  ( -0.40)
+TRANSMEM    121    141     21 1.00 1.60
+EXT_LOOP    142    153     12              1.00  (  0.44)
+TRANSMEM    154    174     21 1.00 2.31
+CYT_LOOP    175    193     19              4.00  (  0.03)
+TRANSMEM    194    214     21 1.00 1.59
+EXT_LOOP    215    221      7              1.00  ( -0.04)
+//
+
+Sequence : gi_90111295_ref_NP_416074_4_  (71 res)
+MKSMDKLTTGVAYGTSAGNAGFWALQLLDKVTPSQWAAIGVLGSLVFGLLTYLTNLYFKI
+KEDRRKAARGE
+
+Found: 2 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1     7 - 27    0.953 Putative
+     2    34 - 54    1.875 Certain
+
+Total of 2 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  0.88         7.00     0.00      1.00    -0.33
+TOPOLOGY                                   N-in        ?      N-in
+CYT_LOOP      1      6      6              2.00  ( -0.07)
+TRANSMEM      7     27     21 0.88 0.95
+EXT_LOOP     28     33      6              1.00  (  0.27)
+TRANSMEM     34     54     21 1.00 1.87
+CYT_LOOP     55     71     17              6.00  ( -0.90)
+//
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   2                  1.00        -3.00     0.00     -3.00    -0.20
+TOPOLOGY                                  N-out        ?     N-out
+EXT_LOOP      1     33     33              3.00  (  0.64)
+TRANSMEM     34     54     21 1.00 1.87
+CYT_LOOP     55     71     17              6.00  ( -0.90)
+//
+
+Sequence : gi_16129540_ref_NP_416099_1_  (108 res)
+MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLALFVWLLTLHPAASGRVY
+AAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIVAGWGRT
+
+Found: 4 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1     4 - 24    1.675 Certain
+     2    31 - 51    2.150 Certain
+     3    58 - 78    1.017 Certain
+     4    86 - 106   1.952 Certain
+
+Total of 1 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  1.00        -1.00     0.00     -1.00    -1.00
+TOPOLOGY                                  N-out        ?     N-out
+EXT_LOOP      1      3      3              1.00  ( -0.18)
+TRANSMEM      4     24     21 1.00 1.67
+CYT_LOOP     25     30      6              2.00  ( -0.11)
+TRANSMEM     31     51     21 1.00 2.15
+EXT_LOOP     52     57      6              1.00  (  0.78)
+TRANSMEM     58     78     21 1.00 1.02
+CYT_LOOP     79     85      7              2.00  ( -0.08)
+TRANSMEM     86    106     21 1.00 1.95
+EXT_LOOP    107    108      2              1.00  ( -0.95)
+//
+
+Sequence : gi_90111300_ref_NP_416103_2_  (101 res)
+MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPESSFTLSIVPNDQVDQP
+DSQVVGHCANDTHKILYTRTTSGNVSAPAQSSQDGAPAEPQ
+
+Found: 1 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1     3 - 23    1.825 Certain
+
+Total of 1 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  1.00         1.00    -0.69      1.00    -1.00
+TOPOLOGY                                   N-in     N-in      N-in
+CYT_LOOP      1      2      2              1.00  ( -0.06)
+TRANSMEM      3     23     21 1.00 1.83
+EXT_LOOP     24    101     78           (  4.00)    0.69
+//
+
+Sequence : gi_90111309_ref_NP_416156_4_  (109 res)
+MTMKKLLIIILPVLLSGCSAFNQLVERMQTDTLEYQCDEKPLTVKLNNPRQEVSFVYDNQ
+LLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNCQLQNPQR
+
+Found: 1 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1     5 - 25    1.526 Certain
+
+Total of 1 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  1.00         3.00    -0.12      6.00    -1.00
+TOPOLOGY                                   N-in     N-in      N-in
+CYT_LOOP      1      4      4              2.00  ( -0.16)
+TRANSMEM      5     25     21 1.00 1.53
+EXT_LOOP     26    109     84           ( 11.00)    0.12
+//
+
+Sequence : gi_90111313_ref_NP_416166_2_  (171 res)
+MGLSELLKTAEVPKGSFYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILA
+YYQQTLNQFCQHGTISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENH
+CLTFCGEPLQQAQVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV
+
+Found: 0 segments
+
+
+Sequence : gi_16129623_ref_NP_416182_1_  (101 res)
+MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTESEKNHEAGGIYLFTDEK
+SALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA
+
+Found: 0 segments
+
+
+Sequence : gi_16129732_ref_NP_416292_1_  (137 res)
+MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVYHCLICDAPLFHSQTKY
+DSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHLGHVFPDGPQPTGERYCVN
+SASLRFTDGENGEEING
+
+Found: 0 segments
+
+
+Sequence : gi_90111344_ref_NP_416352_4_  (218 res)
+MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPWRDLLISVGDVIDRGPQ
+SLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNGGDWFIALADNQQKQAKTALE
+KCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQVLWSRSRLGERQKGQGITGA
+DHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
+
+Found: 0 segments
+
+
+Sequence : gi_90111352_ref_NP_416389_4_  (188 res)
+MANWQSIDELQDIASDLPRFIHALDELSRRLGLNITPLTADHISLRCHQNATAERWRRGF
+EQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPGEKRYPHEGWEHIEIVLPG
+DPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNPTLAVTDGKTTIKFHPWSIEE
+IVASEQSA
+
+Found: 0 segments
+
+
+Sequence : gi_16129855_ref_NP_416418_1_  (165 res)
+MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLRRHAQEEMTHMQRLFDY
+LTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKINELAHAAMTNQDYPTFNFL
+QWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELSTLDTQN
+
+Found: 0 segments
+
+
+Sequence : gi_16129862_ref_NP_416425_1_  (74 res)
+MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLKMEKQLALIEDETQAAV
+FSKTVKQIKQAYRQ
+
+Found: 1 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1    19 - 39    0.614 Putative
+
+Total of 2 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  0.03        -5.00     0.00     -3.00     0.60
+TOPOLOGY                                  N-out        ?     N-out
+EXT_LOOP      1     18     18              1.00  ( -0.12)
+TRANSMEM     19     39     21 0.03 0.61
+CYT_LOOP     40     74     35              7.00  ( -0.23)
+//
+
+Sequence : gi_16129891_ref_NP_416454_1_  (154 res)
+MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADDKAQQRVVPSPVFYALD
+TFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLLLLFSRQDAAVLATEEGKK
+NLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR
+
+Found: 1 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1    13 - 33    1.995 Certain
+
+Total of 1 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  1.00         5.00    -0.22      4.00    -0.60
+TOPOLOGY                                   N-in     N-in      N-in
+CYT_LOOP      1     12     12              4.00  (  0.03)
+TRANSMEM     13     33     21 1.00 1.99
+EXT_LOOP     34    154    121           ( 15.00)    0.22
+//
+
+Sequence : gi_90111376_ref_NP_416555_2_  (159 res)
+MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGYWFVPGGRVQKDETLEA
+AFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHYVVLGFRFRVSEEELLLPD
+EQHDDYRWLTSDALLASDNVHANSRAYFLAEKRTGVPGL
+
+Found: 0 segments
+
+
+Sequence : gi_16130023_ref_NP_416587_1_  (48 res)
+MSGFKRVIDEQDWTITKVTHFLNNSGFTTSLELEVRLSDVGYETEDNE
+
+Found: 0 segments
+
+
+Sequence : gi_16130042_ref_NP_416607_1_  (262 res)
+MQVDLLGSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLALGASPAMVIETEEASQF
+AAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVAVGALDYRRHFCHELLSFK
+PAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTLARETGAIVVVTGEMDYVTDG
+HRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTLENVASACHWMKQAGERAVARSE
+GPGSFVPHFLDALWQLTQEVQA
+
+Found: 3 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1    32 - 52    1.220 Certain
+     2    57 - 77    1.268 Certain
+     3   195 - 215   1.663 Certain
+
+Total of 1 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  1.00        -2.00     0.01      2.00     1.00
+TOPOLOGY                                  N-out    N-out      N-in
+EXT_LOOP      1     31     31              0.00  (  0.28)
+TRANSMEM     32     52     21 1.00 1.22
+CYT_LOOP     53     56      4              0.00  ( -0.90)
+TRANSMEM     57     77     21 1.00 1.27
+EXT_LOOP     78    194    117           ( 11.00)    0.01
+TRANSMEM    195    215     21 1.00 1.66
+CYT_LOOP    216    262     47              3.00  (  0.33)
+//
+
+Sequence : gi_16130217_ref_NP_416785_1_  (325 res)
+MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQNRYGPNRVGWGGSLQL
+VADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVPVSPGWVVADLNIGILFFL
+MMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFLGLSLMGVVAQAGSFNMTDIV
+NSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAEQELADGYHIEYSGMKFGLFFVG
+EYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTAFFMMMFILIRASLPRPRYDQVMSF
+GWKICLPLTLINLLVTAAVILWQAQ
+
+Found: 8 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1    16 - 36    2.018 Certain
+     2    81 - 101   2.245 Certain
+     3   114 - 134   2.358 Certain
+     4   157 - 177   1.366 Certain
+     5   188 - 208   1.885 Certain
+     6   242 - 262   2.100 Certain
+     7   265 - 285   1.991 Certain
+     8   304 - 324   1.661 Certain
+
+Total of 1 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  1.00       -14.00     0.00     -3.00     0.33
+TOPOLOGY                                  N-out        ?     N-out
+EXT_LOOP      1     15     15              1.00  ( -0.28)
+TRANSMEM     16     36     21 1.00 2.02
+CYT_LOOP     37     80     44              8.00  (  0.24)
+TRANSMEM     81    101     21 1.00 2.24
+EXT_LOOP    102    113     12              0.00  (  1.69)
+TRANSMEM    114    134     21 1.00 2.36
+CYT_LOOP    135    156     22              2.00  (  0.82)
+TRANSMEM    157    177     21 1.00 1.37
+EXT_LOOP    178    187     10              0.00  (  1.10)
+TRANSMEM    188    208     21 1.00 1.89
+CYT_LOOP    209    241     33              2.00  (  0.25)
+TRANSMEM    242    262     21 1.00 2.10
+EXT_LOOP    263    264      2              0.00  (  0.16)
+TRANSMEM    265    285     21 1.00 1.99
+CYT_LOOP    286    303     18              4.00  (  0.71)
+TRANSMEM    304    324     21 1.00 1.66
+EXT_LOOP    325    325      1              0.00  ( -0.13)
+//
+
+Sequence : gi_16130268_ref_NP_416836_1_  (179 res)
+MNKSMIQSGGYVLLAGLILAMSSTLFAADNNLHFSGNLLSKSCALVVDGQYLAEVRFPTV
+SRQDLNVAGQSARVPVVFKLKDCKGPAGYNVKVTLTGVEDSEQPGFLALDTSSTAQGVGI
+GMEKTDGMQVAINNTNGATFALTNGNNDINFRAWLQAKSGRDVTIGEFTASLTATFEYI
+
+Found: 1 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1     8 - 28    1.953 Certain
+
+Total of 1 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  1.00         2.00    -1.03      1.00    -1.00
+TOPOLOGY                                   N-in     N-in      N-in
+CYT_LOOP      1      7      7              1.00  (  0.15)
+TRANSMEM      8     28     21 1.00 1.95
+EXT_LOOP     29    179    151           ( 12.00)    1.03
+//
+
+Sequence : gi_16130299_ref_NP_416868_1_  (512 res)
+MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTISGFLGASTDEGTWVIT
+SFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSLSTNLDVLIFFRVVQGLMA
+GPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPILGGYICDNFSWGWIFLINVP
+MGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVGGLQIMLDKGRDLDWFNSSTIII
+LTVVSVISLISLVIWESTSENPILDLSLFKSRNFTIGIVSITCAYLFYSGAIVLMPQLLQ
+ETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNKIDMRLLVTFSFLMYAVCYYWRSVTFM
+PTIDFTGIILPQFFQGFAVACFFLPLTTISFSGLPDNKFANASSMSNFFRTLSGSVGTSL
+TMTLWGRRESLHHSQLTATIDQFNPVFNSSSQIMDKYYGSLSGVLNEINNEITQQSLSIS
+ANEIFRMAAIAFILLTVLVWFAKPPFTAKGVG
+
+Found: 14 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1    12 - 32    1.904 Certain
+     2    54 - 74    1.414 Certain
+     3    85 - 105   1.983 Certain
+     4   112 - 132   0.914 Putative
+     5   141 - 161   2.023 Certain
+     6   171 - 191   1.953 Certain
+     7   205 - 225   1.514 Certain
+     8   235 - 255   2.229 Certain
+     9   276 - 296   1.728 Certain
+    10   308 - 328   1.724 Certain
+    11   337 - 357   1.079 Certain
+    12   374 - 394   2.007 Certain
+    13   407 - 427   0.936 Putative
+    14   484 - 504   1.811 Certain
+
+Total of 4 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  0.78        12.00     0.00      2.00    -1.00
+TOPOLOGY                                   N-in        ?      N-in
+CYT_LOOP      1     11     11              1.00  (  0.07)
+TRANSMEM     12     32     21 1.00 1.90
+EXT_LOOP     33     53     21              0.00  (  0.52)
+TRANSMEM     54     74     21 1.00 1.41
+CYT_LOOP     75     84     10              3.00  ( -1.39)
+TRANSMEM     85    105     21 1.00 1.98
+EXT_LOOP    106    111      6              1.00  (  0.02)
+TRANSMEM    112    132     21 0.78 0.91
+CYT_LOOP    133    140      8              3.00  (  0.14)
+TRANSMEM    141    161     21 1.00 2.02
+EXT_LOOP    162    170      9              0.00  (  0.20)
+TRANSMEM    171    191     21 1.00 1.95
+CYT_LOOP    192    204     13              3.00  ( -0.19)
+TRANSMEM    205    225     21 1.00 1.51
+EXT_LOOP    226    234      9              2.00  ( -0.33)
+TRANSMEM    235    255     21 1.00 2.23
+CYT_LOOP    256    275     20              2.00  (  0.04)
+TRANSMEM    276    296     21 1.00 1.73
+EXT_LOOP    297    307     11              0.00  ( -0.16)
+TRANSMEM    308    328     21 1.00 1.72
+CYT_LOOP    329    336      8              3.00  (  0.10)
+TRANSMEM    337    357     21 1.00 1.08
+EXT_LOOP    358    373     16              1.00  ( -0.13)
+TRANSMEM    374    394     21 1.00 2.01
+CYT_LOOP    395    406     12              2.00  (  0.79)
+TRANSMEM    407    427     21 0.84 0.94
+EXT_LOOP    428    483     56              4.00  (  0.72)
+TRANSMEM    484    504     21 1.00 1.81
+CYT_LOOP    505    512      8              2.00  (  1.11)
+//
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   2                  0.78        10.00     0.86      2.00    -1.00
+TOPOLOGY                                   N-in    N-out      N-in
+CYT_LOOP      1     11     11              1.00  (  0.07)
+TRANSMEM     12     32     21 1.00 1.90
+EXT_LOOP     33     53     21              0.00  (  0.52)
+TRANSMEM     54     74     21 1.00 1.41
+CYT_LOOP     75     84     10              3.00  ( -1.39)
+TRANSMEM     85    105     21 1.00 1.98
+EXT_LOOP    106    111      6              1.00  (  0.02)
+TRANSMEM    112    132     21 0.78 0.91
+CYT_LOOP    133    140      8              3.00  (  0.14)
+TRANSMEM    141    161     21 1.00 2.02
+EXT_LOOP    162    170      9              0.00  (  0.20)
+TRANSMEM    171    191     21 1.00 1.95
+CYT_LOOP    192    204     13              3.00  ( -0.19)
+TRANSMEM    205    225     21 1.00 1.51
+EXT_LOOP    226    234      9              2.00  ( -0.33)
+TRANSMEM    235    255     21 1.00 2.23
+CYT_LOOP    256    275     20              2.00  (  0.04)
+TRANSMEM    276    296     21 1.00 1.73
+EXT_LOOP    297    307     11              0.00  ( -0.16)
+TRANSMEM    308    328     21 1.00 1.72
+CYT_LOOP    329    336      8              3.00  (  0.10)
+TRANSMEM    337    357     21 1.00 1.08
+EXT_LOOP    358    373     16              1.00  ( -0.13)
+TRANSMEM    374    394     21 1.00 2.01
+CYT_LOOP    395    483     89           (  6.00)    0.86
+TRANSMEM    484    504     21 1.00 1.81
+EXT_LOOP    505    512      8              2.00  (  1.11)
+//
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   3                  0.84        -4.00     0.00      2.00    -1.00
+TOPOLOGY                                  N-out        ?      N-in
+EXT_LOOP      1     11     11              1.00  (  0.07)
+TRANSMEM     12     32     21 1.00 1.90
+CYT_LOOP     33     53     21              0.00  (  0.52)
+TRANSMEM     54     74     21 1.00 1.41
+EXT_LOOP     75     84     10              3.00  ( -1.39)
+TRANSMEM     85    105     21 1.00 1.98
+CYT_LOOP    106    140     35              4.00  (  0.53)
+TRANSMEM    141    161     21 1.00 2.02
+EXT_LOOP    162    170      9              0.00  (  0.20)
+TRANSMEM    171    191     21 1.00 1.95
+CYT_LOOP    192    204     13              3.00  ( -0.19)
+TRANSMEM    205    225     21 1.00 1.51
+EXT_LOOP    226    234      9              2.00  ( -0.33)
+TRANSMEM    235    255     21 1.00 2.23
+CYT_LOOP    256    275     20              2.00  (  0.04)
+TRANSMEM    276    296     21 1.00 1.73
+EXT_LOOP    297    307     11              0.00  ( -0.16)
+TRANSMEM    308    328     21 1.00 1.72
+CYT_LOOP    329    336      8              3.00  (  0.10)
+TRANSMEM    337    357     21 1.00 1.08
+EXT_LOOP    358    373     16              1.00  ( -0.13)
+TRANSMEM    374    394     21 1.00 2.01
+CYT_LOOP    395    406     12              2.00  (  0.79)
+TRANSMEM    407    427     21 0.84 0.94
+EXT_LOOP    428    483     56              4.00  (  0.72)
+TRANSMEM    484    504     21 1.00 1.81
+CYT_LOOP    505    512      8              2.00  (  1.11)
+//
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   4                  1.00        -2.00    -0.86      2.00    -1.00
+TOPOLOGY                                  N-out     N-in      N-in
+EXT_LOOP      1     11     11              1.00  (  0.07)
+TRANSMEM     12     32     21 1.00 1.90
+CYT_LOOP     33     53     21              0.00  (  0.52)
+TRANSMEM     54     74     21 1.00 1.41
+EXT_LOOP     75     84     10              3.00  ( -1.39)
+TRANSMEM     85    105     21 1.00 1.98
+CYT_LOOP    106    140     35              4.00  (  0.53)
+TRANSMEM    141    161     21 1.00 2.02
+EXT_LOOP    162    170      9              0.00  (  0.20)
+TRANSMEM    171    191     21 1.00 1.95
+CYT_LOOP    192    204     13              3.00  ( -0.19)
+TRANSMEM    205    225     21 1.00 1.51
+EXT_LOOP    226    234      9              2.00  ( -0.33)
+TRANSMEM    235    255     21 1.00 2.23
+CYT_LOOP    256    275     20              2.00  (  0.04)
+TRANSMEM    276    296     21 1.00 1.73
+EXT_LOOP    297    307     11              0.00  ( -0.16)
+TRANSMEM    308    328     21 1.00 1.72
+CYT_LOOP    329    336      8              3.00  (  0.10)
+TRANSMEM    337    357     21 1.00 1.08
+EXT_LOOP    358    373     16              1.00  ( -0.13)
+TRANSMEM    374    394     21 1.00 2.01
+CYT_LOOP    395    483     89           (  6.00)    0.86
+TRANSMEM    484    504     21 1.00 1.81
+EXT_LOOP    505    512      8              2.00  (  1.11)
+//
+
+Sequence : gi_90111436_ref_NP_416933_4_  (166 res)
+MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTLVVEGDLAACRAALDAG
+CAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPDAPVIVAESADELLALLTS
+VRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSRYRLKPH
+
+Found: 0 segments
+
+
+Sequence : gi_90111440_ref_NP_416957_4_  (111 res)
+MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIMTLTPGETAMIAGDLAL
+KAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLNYTLCEMTKS
+
+Found: 1 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1    38 - 58    1.022 Certain
+
+Total of 1 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  1.00         2.00     0.00      0.00     0.09
+TOPOLOGY                                   N-in        ?         ?
+CYT_LOOP      1     37     37              5.00  ( -0.01)
+TRANSMEM     38     58     21 1.00 1.02
+EXT_LOOP     59    111     53              4.00  (  0.69)
+//
+
+Sequence : gi_90111445_ref_NP_416978_4_  (315 res)
+MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRGPGIWQDYRDIHKLFKR
+QEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGGGDLITLIYLLALFRFFFA
+LSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIAGSTHIEMISNTLAMGWNSPL
+TTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTEYSGAGLALAKWGLGLKQVVMAS
+LFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFVLASIAENTLARGRFLLIHHVTWLG
+FSLAALAWVFWLTGL
+
+Found: 8 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1     8 - 28    2.008 Certain
+     2    77 - 97    2.254 Certain
+     3   104 - 124   1.455 Certain
+     4   145 - 165   1.884 Certain
+     5   178 - 198   1.880 Certain
+     6   232 - 252   1.456 Certain
+     7   260 - 280   1.780 Certain
+     8   295 - 315   2.082 Certain
+
+Total of 1 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  1.00       -14.00     0.00     -4.00     0.00
+TOPOLOGY                                  N-out        ?     N-out
+EXT_LOOP      1      7      7              1.00  ( -1.66)
+TRANSMEM      8     28     21 1.00 2.01
+CYT_LOOP     29     76     48             10.00  ( -0.23)
+TRANSMEM     77     97     21 1.00 2.25
+EXT_LOOP     98    103      6              0.00  (  0.67)
+TRANSMEM    104    124     21 1.00 1.46
+CYT_LOOP    125    144     20              1.00  (  0.17)
+TRANSMEM    145    165     21 1.00 1.88
+EXT_LOOP    166    177     12              0.00  (  0.12)
+TRANSMEM    178    198     21 1.00 1.88
+CYT_LOOP    199    231     33              3.00  ( -0.16)
+TRANSMEM    232    252     21 1.00 1.46
+EXT_LOOP    253    259      7              1.00  ( -1.09)
+TRANSMEM    260    280     21 1.00 1.78
+CYT_LOOP    281    294     14              2.00  ( -0.79)
+TRANSMEM    295    315     21 1.00 2.08
+//
+
+Sequence : gi_16130429_ref_NP_416999_1_  (63 res)
+MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHHQSKKEAQQSTLSVESP
+VQR
+
+Found: 1 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1    20 - 40    1.458 Certain
+
+Total of 1 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  1.00         4.00     0.00      5.00    -1.00
+TOPOLOGY                                   N-in        ?      N-in
+CYT_LOOP      1     19     19              6.00  ( -0.24)
+TRANSMEM     20     40     21 1.00 1.46
+EXT_LOOP     41     63     23              3.00  (  0.59)
+//
+
+Sequence : gi_16130443_ref_NP_417013_1_  (143 res)
+MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTVEQARGFYAEHDGKPFF
+DGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGTLRADYADSLTENGTHGSD
+SVESAAREIAYFFGEGEVCPRTR
+
+Found: 1 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1    58 - 78    0.861 Putative
+
+Total of 2 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  0.65         9.00     0.28     -1.00    -0.23
+TOPOLOGY                                   N-in    N-out     N-out
+CYT_LOOP      1     57     57              8.00  (  0.41)
+TRANSMEM     58     78     21 0.65 0.86
+EXT_LOOP     79    143     65           (  6.00)   -0.28
+//
+
+Sequence : gi_16130511_ref_NP_417081_1_  (90 res)
+MANDSWSGQDKAQHFIASAMLSAAGNEYSQHQGMSRDRSAMFGLMFSVSLGASKELWDSR
+PEGSGWSWKDLAWDVAGASTGYTVWQLTRH
+
+Found: 1 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1    37 - 57    0.884 Putative
+
+Total of 2 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  0.71         0.00     0.00      1.00     0.00
+TOPOLOGY                                   N-in        ?      N-in
+    LOOP      1     36     36              3.00  (  0.49)
+TRANSMEM     37     57     21 0.71 0.88
+    LOOP     58     90     33              4.00  (  0.48)
+//
+
+Sequence : gi_49176253_ref_YP_026176_1_  (66 res)
+MAAGFIADTASLPLIVSNLVNIVSADFFGLSFAQYASVMISVDAAAIAATLIMLYLFFRR
+VIPATY
+
+Found: 2 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1     1 - 21    0.901 Putative
+     2    38 - 58    1.450 Certain
+
+Total of 2 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  0.75         2.00     0.00      1.00     0.00
+TOPOLOGY                                   N-in        ?      N-in
+TRANSMEM      1     21     21 0.75 0.90
+EXT_LOOP     22     37     16              0.00  (  0.90)
+TRANSMEM     38     58     21 1.00 1.45
+CYT_LOOP     59     66      8              2.00  (  0.34)
+//
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   2                  1.00        -1.00     0.00     -3.00     1.00
+TOPOLOGY                                      ?        ?     N-out
+EXT_LOOP      1     37     37              0.00  (  1.08)
+TRANSMEM     38     58     21 1.00 1.45
+CYT_LOOP     59     66      8              2.00  (  0.34)
+//
+
+Sequence : gi_16130584_ref_NP_417156_1_  (149 res)
+MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYSRLVAIPVNILIAWPYG
+MYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLVVGADWHQIMAAVSSNIVV
+SMLMGAVYGYFLDYCRRLFKVSRYQQVKA
+
+Found: 4 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1    14 - 34    1.150 Certain
+     2    42 - 62    0.819 Putative
+     3    85 - 105   1.445 Certain
+     4   109 - 129   1.568 Certain
+
+Total of 2 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  0.55        11.00     0.00      2.00    -1.00
+TOPOLOGY                                   N-in        ?      N-in
+CYT_LOOP      1     13     13              2.00  (  0.60)
+TRANSMEM     14     34     21 1.00 1.15
+EXT_LOOP     35     41      7              1.00  (  0.45)
+TRANSMEM     42     62     21 0.55 0.82
+CYT_LOOP     63     84     22              5.00  ( -0.03)
+TRANSMEM     85    105     21 1.00 1.44
+EXT_LOOP    106    108      3              0.00  (  0.06)
+TRANSMEM    109    129     21 1.00 1.57
+CYT_LOOP    130    149     20              5.00  (  0.30)
+//
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   2                  1.00        -9.00     0.00      2.00    -1.00
+TOPOLOGY                                  N-out        ?      N-in
+EXT_LOOP      1     13     13              2.00  (  0.60)
+TRANSMEM     14     34     21 1.00 1.15
+CYT_LOOP     35     84     50              6.00  (  1.11)
+TRANSMEM     85    105     21 1.00 1.44
+EXT_LOOP    106    108      3              0.00  (  0.06)
+TRANSMEM    109    129     21 1.00 1.57
+CYT_LOOP    130    149     20              5.00  (  0.30)
+//
+
+Sequence : gi_16130625_ref_NP_417198_1_  (136 res)
+MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDCLEAALTCPWDEYLAWI
+ATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAAQTSQQQEWSTMLLSMLHD
+IHQENAIYLMVRRLRD
+
+Found: 0 segments
+
+
+Sequence : gi_16130646_ref_NP_417219_1_  (258 res)
+MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYNYSTLQIQKQLEQNHLTLALFN
+TAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQVHVMAGVVPAGEDAERYRA
+VFIDNIRYAADRFAPHGKRILVEALSPGVKPHYLFSSQYQALAIVEEVARDNVFIQLDTF
+HAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGEINYPWLFRLFDEVGYQGWIGCE
+YKPRGLTEEGLGWFDAWR
+
+Found: 0 segments
+
+
+Sequence : gi_16130699_ref_NP_417272_1_  (109 res)
+MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDTMEPLEWLQWVLIPRMH
+DLLDNKQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDALFADDAS
+
+Found: 0 segments
+
+
+Sequence : gi_16130761_ref_NP_417334_1_  (143 res)
+MINDLKSILLKPSEEVDVFIKIFESWVTKLPSISGPVNLHIPTSFKDKSLEVESYFVDKS
+IWNVHITFHDDKRFVFFTDQFIAEFSPQEFVDNCEQYLINNHCFSPDKVNEICEQARHYL
+VEKMFETHSLDMNNSVLASPEDL
+
+Found: 0 segments
+
+
+Sequence : gi_16130806_ref_NP_417380_1_  (129 res)
+MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVDLPEVGATVSAGDDCAV
+AESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWIFKIKASDESELESLLDAT
+AYEALLEDE
+
+Found: 0 segments
+
+
+Sequence : gi_16130817_ref_NP_417391_1_  (297 res)
+MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLENMFGQPLLVRTVPPRPT
+EQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSLATWLLPALAPVLADSPIR
+LNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLGALDYLFVSSKPFAEKYFPNG
+VTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPCHIVNSSEAFVQLARQGTTCCMI
+PHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPESRMMRKVTDALLDYGHKVLRQD
+
+Found: 1 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1    99 - 119   1.022 Certain
+
+Total of 1 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  1.00         0.00    -0.75      1.00    -0.04
+TOPOLOGY                                      ?     N-in      N-in
+    LOOP      1     98     98           ( 13.00)   -0.67
+TRANSMEM     99    119     21 1.00 1.02
+    LOOP    120    297    178           ( 20.00)    0.08
+//
+
+Sequence : gi_16130835_ref_NP_417409_1_  (147 res)
+MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQAIKDSTINNGPYYILA
+PGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIGLSAADADSHIGAIQALSE
+LLCEEEILEQLLTASSEKQLADIISRG
+
+Found: 0 segments
+
+
+Sequence : gi_16130863_ref_NP_417437_1_  (91 res)
+MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQHKQTMLINEKKLNMMNA
+EHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK
+
+Found: 0 segments
+
+
+Sequence : gi_16130867_ref_NP_417441_1_  (235 res)
+MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLGIGILLVVKCANLPAMV
+LATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESFIQNYVAIIVLFCASGTGI
+FGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVISIPLLIIQLTLAWAAALILP
+LTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLPALLLAMPLSAAWTAWFA
+
+Found: 6 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1     2 - 22    1.564 Certain
+     2    32 - 52    2.021 Certain
+     3   105 - 125   1.969 Certain
+     4   143 - 163   2.161 Certain
+     5   168 - 188   1.736 Certain
+     6   215 - 235   1.822 Certain
+
+Total of 1 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  1.00         2.00     0.00     -2.00     0.00
+TOPOLOGY                                   N-in        ?     N-out
+CYT_LOOP      1      1      1              0.00  ( -0.06)
+TRANSMEM      2     22     21 1.00 1.56
+EXT_LOOP     23     31      9              3.00  ( -0.89)
+TRANSMEM     32     52     21 1.00 2.02
+CYT_LOOP     53    104     52              7.00  ( -0.21)
+TRANSMEM    105    125     21 1.00 1.97
+EXT_LOOP    126    142     17              1.00  ( -0.34)
+TRANSMEM    143    163     21 1.00 2.16
+CYT_LOOP    164    167      4              0.00  ( -0.54)
+TRANSMEM    168    188     21 1.00 1.74
+EXT_LOOP    189    214     26              2.00  (  0.31)
+TRANSMEM    215    235     21 1.00 1.82
+//
+
+Sequence : gi_16130900_ref_NP_417475_1_  (164 res)
+MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPNIFSMAESDLILVLLSL
+VDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDATSLKNKVAASIVAISSIH
+LLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLTRHNH
+
+Found: 4 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1    14 - 34    1.715 Certain
+     2    58 - 78    1.759 Certain
+     3   107 - 127   0.675 Putative
+     4   136 - 156   1.976 Certain
+
+Total of 2 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  1.00         8.00     0.00      0.00     0.00
+TOPOLOGY                                   N-in        ?         ?
+CYT_LOOP      1     13     13              2.00  ( -0.25)
+TRANSMEM     14     34     21 1.00 1.71
+EXT_LOOP     35     57     23              1.00  (  0.31)
+TRANSMEM     58     78     21 1.00 1.76
+CYT_LOOP     79    135     57              8.00  (  0.79)
+TRANSMEM    136    156     21 1.00 1.98
+EXT_LOOP    157    164      8              2.00  ( -0.47)
+//
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   2                  0.19         6.00     0.00      0.00     0.00
+TOPOLOGY                                   N-in        ?         ?
+CYT_LOOP      1     13     13              2.00  ( -0.25)
+TRANSMEM     14     34     21 1.00 1.71
+EXT_LOOP     35     57     23              1.00  (  0.31)
+TRANSMEM     58     78     21 1.00 1.76
+CYT_LOOP     79    106     28              5.00  (  0.03)
+TRANSMEM    107    127     21 0.19 0.67
+EXT_LOOP    128    135      8              3.00  (  0.72)
+TRANSMEM    136    156     21 1.00 1.98
+CYT_LOOP    157    164      8              2.00  ( -0.47)
+//
+
+Sequence : gi_16130902_ref_NP_417477_1_  (81 res)
+MNALSGLQTHEDSTCCNRFCRPDERSASGNSTLLRFGGFFADQTYLQVTRLMQRIHYLHQ
+RLVIDGFVRSEEDGGVFLAFG
+
+Found: 0 segments
+
+
+Sequence : gi_16130905_ref_NP_417480_1_  (51 res)
+MLVTFLLRKRKEKKAKVRQYANSNENDYQFDVVLILLCADFVTCVLEIHSG
+
+Found: 1 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1    29 - 49    0.982 Putative
+
+Total of 2 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  0.96         8.00     0.00      1.00    -0.45
+TOPOLOGY                                   N-in        ?      N-in
+CYT_LOOP      1     28     28              8.00  (  0.20)
+TRANSMEM     29     49     21 0.96 0.98
+EXT_LOOP     50     51      2              0.00  (  0.93)
+//
+
+Sequence : gi_16130984_ref_NP_417560_1_  (414 res)
+MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVGLLGTLFVGALKAVAPI
+LVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVFSFAFPSTLHLSSSAGDIS
+PPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLGFALRHGNETTKNLVNDMSNA
+VTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQLLVVLVGCMLLVALVVNPLLVWW
+KIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMALCEKLNLDRDTYSVSIPLGATINM
+AGAAITITVLTLAAVNTLGIPVDLPTALLLSVVASLCACGASGVAGGSLLLIPLACNMFG
+ISNDIAMQVVAVGFIIGVLQDSCETALNSSTDVLFTAAACQAEDDRLANSALRN
+
+Found: 9 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1    18 - 38    1.479 Certain
+     2    52 - 72    1.755 Certain
+     3    88 - 108   2.057 Certain
+     4   146 - 166   1.213 Certain
+     5   191 - 211   1.817 Certain
+     6   216 - 236   2.077 Certain
+     7   295 - 315   1.700 Certain
+     8   324 - 344   1.835 Certain
+     9   361 - 381   1.065 Certain
+
+Total of 1 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  1.00        12.00     0.00      4.00    -1.00
+TOPOLOGY                                   N-in        ?      N-in
+CYT_LOOP      1     17     17              4.00  ( -0.17)
+TRANSMEM     18     38     21 1.00 1.48
+EXT_LOOP     39     51     13              2.00  (  0.43)
+TRANSMEM     52     72     21 1.00 1.76
+CYT_LOOP     73     87     15              2.00  ( -0.04)
+TRANSMEM     88    108     21 1.00 2.06
+EXT_LOOP    109    145     37              2.00  (  1.06)
+TRANSMEM    146    166     21 1.00 1.21
+CYT_LOOP    167    190     24              4.00  (  0.71)
+TRANSMEM    191    211     21 1.00 1.82
+EXT_LOOP    212    215      4              0.00  (  0.55)
+TRANSMEM    216    236     21 1.00 2.08
+CYT_LOOP    237    294     58              7.00  (  0.54)
+TRANSMEM    295    315     21 1.00 1.70
+EXT_LOOP    316    323      8              0.00  (  0.51)
+TRANSMEM    324    344     21 1.00 1.84
+CYT_LOOP    345    360     16              0.00  ( -0.17)
+TRANSMEM    361    381     21 1.00 1.06
+EXT_LOOP    382    414     33              2.00  ( -0.86)
+//
+
+Sequence : gi_16131011_ref_NP_417588_1_  (312 res)
+MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIINDIEDYFGVELVVRKNTG
+VTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFGFPSLIGFTFMSGMINKFK
+EVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKLQDLHVEPLFESEFVLVASKS
+RTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNGISIENIVKTDSVVTIYNLVLNA
+DFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAVWSKNYRIKKAASVLVELAKEYSSY
+NGCRRRQLIEVG
+
+Found: 1 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1    97 - 117   1.849 Certain
+
+Total of 1 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  1.00         0.00     0.03     -4.00     0.05
+TOPOLOGY                                      ?    N-out     N-out
+    LOOP      1     96     96           (  9.00)    0.94
+TRANSMEM     97    117     21 1.00 1.85
+    LOOP    118    312    195           ( 19.00)    0.92
+//
+
+Sequence : gi_16131049_ref_NP_417626_1_  (174 res)
+MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQALDDGELEFLEGRWLSI
+HVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAARKQDPDTLFFQRRLVIEG
+DTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMKTAPETKQTSVGEPC
+
+Found: 1 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1     9 - 29    0.747 Putative
+
+Total of 2 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  0.37         3.00     0.25      0.00    -0.50
+TOPOLOGY                                   N-in    N-out         ?
+CYT_LOOP      1      8      8              3.00  ( -0.94)
+TRANSMEM      9     29     21 0.37 0.75
+EXT_LOOP     30    174    145           ( 15.00)   -0.25
+//
+
+Sequence : gi_16131065_ref_NP_417642_1_  (110 res)
+MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFGSSGSGNFMTRMTALLA
+TLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPAKPTSDIPN
+
+Found: 3 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1     3 - 23    2.366 Certain
+     2    29 - 49    1.452 Certain
+     3    54 - 74    1.995 Certain
+
+Total of 1 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  1.00        -3.00     0.00     -1.00     0.00
+TOPOLOGY                                  N-out        ?     N-out
+EXT_LOOP      1      2      2              0.00  (  0.41)
+TRANSMEM      3     23     21 1.00 2.37
+CYT_LOOP     24     28      5              1.00  (  0.18)
+TRANSMEM     29     49     21 1.00 1.45
+EXT_LOOP     50     53      4              1.00  (  0.15)
+TRANSMEM     54     74     21 1.00 1.99
+CYT_LOOP     75    110     36              4.00  (  0.37)
+//
+
+Sequence : gi_90111557_ref_NP_417676_2_  (217 res)
+MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQQVDVINHLTGEAMTET
+RNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLSNFASLGSECTVDRELKAL
+AQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTAEVLEEMGAEHVPCPVDDIVV
+DEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE
+
+Found: 1 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1   127 - 147   0.921 Putative
+
+Total of 2 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  0.80         0.00    -0.30      3.00     0.11
+TOPOLOGY                                      ?     N-in      N-in
+    LOOP      1    126    126           ( 12.00)   -0.32
+TRANSMEM    127    147     21 0.80 0.92
+    LOOP    148    217     70           (  5.00)   -0.02
+//
+
+Sequence : gi_16131149_ref_NP_417727_1_  (98 res)
+MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNGQDVNDLYELVLAEVEQ
+PLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN
+
+Found: 0 segments
+
+
+Sequence : gi_16131155_ref_NP_417733_1_  (73 res)
+MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTTQDNWETAGAIAGGAAA
+VAGLTMGIIALSK
+
+Found: 2 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1     3 - 23    1.013 Certain
+     2    52 - 72    1.877 Certain
+
+Total of 1 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  1.00         1.00     0.00      4.00    -1.00
+TOPOLOGY                                   N-in        ?      N-in
+CYT_LOOP      1      2      2              2.00  ( -0.06)
+TRANSMEM      3     23     21 1.00 1.01
+EXT_LOOP     24     51     28              2.00  (  0.07)
+TRANSMEM     52     72     21 1.00 1.88
+CYT_LOOP     73     73      1              1.00  ( -0.03)
+//
+
+Sequence : gi_16131230_ref_NP_417810_1_  (184 res)
+MMSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHYPDFFIDIPREQALLR
+EHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAGKYWRSVITTGEPESAYR
+YDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSAQELASHARAYGDWLANPLS
+PGGR
+
+Found: 0 segments
+
+
+Sequence : gi_90111586_ref_NP_417860_2_  (292 res)
+MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNVLAELLVATSLLTATLK
+FDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENADLKTLVGNGYVVITITPS
+EGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGDVDGKPAAGGMLLQVMPAQNA
+QQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEVTVYDPQDVEFKCTCSRERCADA
+LKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMDIAEIRNNASPADPQVH
+
+Found: 1 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1    43 - 63    0.770 Putative
+
+Total of 2 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  0.42         4.00     0.55      0.00     0.33
+TOPOLOGY                                   N-in    N-out         ?
+CYT_LOOP      1     42     42              3.00  (  0.54)
+TRANSMEM     43     63     21 0.42 0.77
+EXT_LOOP     64    292    229           ( 16.00)   -0.55
+//
+
+Sequence : gi_16131340_ref_NP_417925_1_  (208 res)
+MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQAPMFDAISYLVLAGLC
+ASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSFFWPLPLVLLVLGALLLAI
+IWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALSAFVGASLLFISNFVWLGSHY
+RRRFRADNAIAAACYFAGHFLIVRSLYL
+
+Found: 7 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1     1 - 21    1.222 Certain
+     2    28 - 48    1.462 Certain
+     3    76 - 96    1.625 Certain
+     4   103 - 123   2.252 Certain
+     5   130 - 150   1.986 Certain
+     6   158 - 178   1.789 Certain
+     7   188 - 208   0.867 Putative
+
+Total of 2 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  1.00         4.00     0.00     -2.00     0.00
+TOPOLOGY                                   N-in        ?     N-out
+TRANSMEM      1     21     21 1.00 1.22
+EXT_LOOP     22     27      6              3.00  (  0.05)
+TRANSMEM     28     48     21 1.00 1.46
+CYT_LOOP     49     75     27              2.00  ( -0.65)
+TRANSMEM     76     96     21 1.00 1.63
+EXT_LOOP     97    102      6              0.00  (  0.06)
+TRANSMEM    103    123     21 1.00 2.25
+CYT_LOOP    124    129      6              2.00  ( -0.88)
+TRANSMEM    130    150     21 1.00 1.99
+EXT_LOOP    151    157      7              1.00  (  0.22)
+TRANSMEM    158    178     21 1.00 1.79
+CYT_LOOP    179    208     30              5.00  ( -0.05)
+//
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   2                  0.67         4.00     0.00     -2.00     0.00
+TOPOLOGY                                   N-in        ?     N-out
+TRANSMEM      1     21     21 1.00 1.22
+EXT_LOOP     22     27      6              3.00  (  0.05)
+TRANSMEM     28     48     21 1.00 1.46
+CYT_LOOP     49     75     27              2.00  ( -0.65)
+TRANSMEM     76     96     21 1.00 1.63
+EXT_LOOP     97    102      6              0.00  (  0.06)
+TRANSMEM    103    123     21 1.00 2.25
+CYT_LOOP    124    129      6              2.00  ( -0.88)
+TRANSMEM    130    150     21 1.00 1.99
+EXT_LOOP    151    157      7              1.00  (  0.22)
+TRANSMEM    158    178     21 1.00 1.79
+CYT_LOOP    179    187      9              4.00  ( -0.56)
+TRANSMEM    188    208     21 0.67 0.87
+//
+
+Sequence : gi_16131343_ref_NP_417928_1_  (221 res)
+MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTWGAFSFPFIFLATDLTV
+RIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALAHFNLFVARIATASFMAYA
+LGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFFIAFWRSPDAFMAEHWMEIAL
+VDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINALQAS
+
+Found: 6 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1    11 - 31    1.427 Certain
+     2    36 - 56    1.804 Certain
+     3    71 - 91    2.016 Certain
+     4   102 - 122   0.864 Putative
+     5   144 - 164   1.160 Certain
+     6   186 - 206   1.900 Certain
+
+Total of 2 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  0.66        11.00     0.00      2.00    -1.00
+TOPOLOGY                                   N-in        ?      N-in
+CYT_LOOP      1     10     10              2.00  (  0.79)
+TRANSMEM     11     31     21 1.00 1.43
+EXT_LOOP     32     35      4              0.00  (  0.90)
+TRANSMEM     36     56     21 1.00 1.80
+CYT_LOOP     57     70     14              3.00  ( -0.69)
+TRANSMEM     71     91     21 1.00 2.02
+EXT_LOOP     92    101     10              0.00  (  0.52)
+TRANSMEM    102    122     21 0.66 0.86
+CYT_LOOP    123    143     21              4.00  (  0.17)
+TRANSMEM    144    164     21 1.00 1.16
+EXT_LOOP    165    185     21              2.00  ( -0.18)
+TRANSMEM    186    206     21 1.00 1.90
+CYT_LOOP    207    221     15              3.00  ( -0.57)
+//
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   2                  1.00         0.00     0.00      2.00    -1.00
+TOPOLOGY                                   N-in        ?      N-in
+    LOOP      1     10     10              2.00  (  0.79)
+TRANSMEM     11     31     21 1.00 1.43
+    LOOP     32     35      4              0.00  (  0.90)
+TRANSMEM     36     56     21 1.00 1.80
+    LOOP     57     70     14              3.00  ( -0.69)
+TRANSMEM     71     91     21 1.00 2.02
+    LOOP     92    143     52              5.00  (  0.56)
+TRANSMEM    144    164     21 1.00 1.16
+    LOOP    165    185     21              2.00  ( -0.18)
+TRANSMEM    186    206     21 1.00 1.90
+    LOOP    207    221     15              3.00  ( -0.57)
+//
+
+Sequence : gi_90111596_ref_NP_417930_2_  (405 res)
+MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYVHDVMGFSAFWAGLVIS
+LQYFATLLSRPHAGRYADSLGPKKIVVFGLCGCFLSGLGYLTAGLTASLPVISLLLLCLG
+RVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTYGAMAMGAPLGVVFYHWGGLQ
+ALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLGRVWLYGMALALASAGFGVIATF
+ITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGINRIGGLNVAMICFSVEIIGLLLVGV
+ATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVPQQNQGAALATYTVFMDLSLGVTGPLA
+GLVMSWAGVPVIYLAAAGLVAIALLLTWRLKKRPPEHVPEAASSS
+
+Found: 12 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1    19 - 39    1.630 Certain
+     2    47 - 67    1.636 Certain
+     3   100 - 120   1.895 Certain
+     4   129 - 149   1.510 Certain
+     5   156 - 176   1.632 Certain
+     6   179 - 199   2.300 Certain
+     7   225 - 245   2.094 Certain
+     8   250 - 270   1.458 Certain
+     9   283 - 303   1.727 Certain
+    10   309 - 329   1.986 Certain
+    11   339 - 359   1.123 Certain
+    12   368 - 388   1.948 Certain
+
+Total of 1 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  1.00        18.00     0.00      2.00     0.00
+TOPOLOGY                                   N-in        ?      N-in
+CYT_LOOP      1     18     18              2.00  ( -0.08)
+TRANSMEM     19     39     21 1.00 1.63
+EXT_LOOP     40     46      7              0.00  (  1.04)
+TRANSMEM     47     67     21 1.00 1.64
+CYT_LOOP     68     99     32              4.00  (  0.40)
+TRANSMEM    100    120     21 1.00 1.89
+EXT_LOOP    121    128      8              1.00  (  0.10)
+TRANSMEM    129    149     21 1.00 1.51
+CYT_LOOP    150    155      6              1.00  (  0.79)
+TRANSMEM    156    176     21 1.00 1.63
+EXT_LOOP    177    178      2              0.00  (  0.41)
+TRANSMEM    179    199     21 1.00 2.30
+CYT_LOOP    200    224     25              6.00  (  1.16)
+TRANSMEM    225    245     21 1.00 2.09
+EXT_LOOP    246    249      4              1.00  ( -0.40)
+TRANSMEM    250    270     21 1.00 1.46
+CYT_LOOP    271    282     12              2.00  (  0.36)
+TRANSMEM    283    303     21 1.00 1.73
+EXT_LOOP    304    308      5              1.00  (  0.30)
+TRANSMEM    309    329     21 1.00 1.99
+CYT_LOOP    330    338      9              1.00  (  0.90)
+TRANSMEM    339    359     21 1.00 1.12
+EXT_LOOP    360    367      8              0.00  (  0.79)
+TRANSMEM    368    388     21 1.00 1.95
+CYT_LOOP    389    405     17              4.00  (  0.55)
+//
+
+Sequence : gi_16131350_ref_NP_417935_1_  (277 res)
+MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSRLLSPDAQHWLGTDHLG
+RDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGRVDQATMRVADMFMTFPTS
+ILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISLRQREFVLASRLSGAGHVRVF
+VDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVTAPTAEWGVMINDARQYIWTQPL
+QMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHAH
+
+Found: 5 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1    13 - 33    1.922 Certain
+     2    75 - 95    2.252 Certain
+     3   113 - 133   2.186 Certain
+     4   202 - 222   1.715 Certain
+     5   239 - 259   1.892 Certain
+
+Total of 1 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  1.00         1.00    -0.84      3.00    -1.00
+TOPOLOGY                                   N-in     N-in      N-in
+CYT_LOOP      1     12     12              2.00  (  0.77)
+TRANSMEM     13     33     21 1.00 1.92
+EXT_LOOP     34     74     41              4.00  ( -0.27)
+TRANSMEM     75     95     21 1.00 2.25
+CYT_LOOP     96    112     17              2.00  ( -0.23)
+TRANSMEM    113    133     21 1.00 2.19
+EXT_LOOP    134    201     68           (  6.00)    0.84
+TRANSMEM    202    222     21 1.00 1.71
+CYT_LOOP    223    238     16              1.00  (  0.15)
+TRANSMEM    239    259     21 1.00 1.89
+EXT_LOOP    260    277     18              1.00  ( -0.21)
+//
+
+Sequence : gi_90111601_ref_NP_417948_2_  (350 res)
+MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLISLAAICLALFTTAFII
+ISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMADEFVAGHVIFGVGMIAAC
+VSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLIAVPVLLTLLGFIWSITLLRS
+ADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTFSTKEHWLWCYWVIFLGSITVLQ
+GIYVLVSSDASARLAPGIILICLGMICYSIFSKVWLLALVWRRTCSLANRIPMIPVFTCL
+FCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCFTLFSIVSILEAGSAKK
+
+Found: 10 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1     8 - 28    1.996 Certain
+     2    42 - 62    2.293 Certain
+     3    74 - 94    1.883 Certain
+     4   107 - 127   1.924 Certain
+     5   158 - 178   2.195 Certain
+     6   191 - 211   2.110 Certain
+     7   223 - 243   1.711 Certain
+     8   254 - 274   1.887 Certain
+     9   291 - 311   2.280 Certain
+    10   325 - 345   2.004 Certain
+
+Total of 1 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  1.00        10.00     0.00      3.00    -1.00
+TOPOLOGY                                   N-in        ?      N-in
+CYT_LOOP      1      7      7              1.00  (  0.47)
+TRANSMEM      8     28     21 1.00 2.00
+EXT_LOOP     29     41     13              0.00  (  1.19)
+TRANSMEM     42     62     21 1.00 2.29
+CYT_LOOP     63     73     11              1.00  (  0.64)
+TRANSMEM     74     94     21 1.00 1.88
+EXT_LOOP     95    106     12              0.00  (  0.38)
+TRANSMEM    107    127     21 1.00 1.92
+CYT_LOOP    128    157     30              2.00  (  1.35)
+TRANSMEM    158    178     21 1.00 2.19
+EXT_LOOP    179    190     12              1.00  (  0.84)
+TRANSMEM    191    211     21 1.00 2.11
+CYT_LOOP    212    222     11              2.00  ( -0.18)
+TRANSMEM    223    243     21 1.00 1.71
+EXT_LOOP    244    253     10              1.00  (  0.94)
+TRANSMEM    254    274     21 1.00 1.89
+CYT_LOOP    275    290     16              4.00  ( -0.41)
+TRANSMEM    291    311     21 1.00 2.28
+EXT_LOOP    312    324     13              1.00  (  0.11)
+TRANSMEM    325    345     21 1.00 2.00
+CYT_LOOP    346    350      5              2.00  (  0.49)
+//
+
+Sequence : gi_16131427_ref_NP_418012_1_  (70 res)
+MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKSLDEGQKVSFTIESGAK
+GPAAGNVTSL
+
+Found: 0 segments
+
+
+Sequence : gi_16131530_ref_NP_418116_1_  (307 res)
+MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRLIFAGLILLTLSFVHGD
+KIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATATVLQFLSPTIIVAWFSLV
+RKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALFWGIASAFAAAFYTTYPSTLI
+ARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLILAFFYLVVIGTSLTFSLYLKGA
+QLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDWLGTLLILSSVILISMDSRRRARKI
+NRPARHK
+
+Found: 9 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1     7 - 27    1.505 Certain
+     2    39 - 59    1.516 Certain
+     3    73 - 93    1.873 Certain
+     4   100 - 120   1.709 Certain
+     5   125 - 145   2.244 Certain
+     6   154 - 174   1.892 Certain
+     7   184 - 204   2.032 Certain
+     8   215 - 235   2.126 Certain
+     9   256 - 276   1.834 Certain
+
+Total of 1 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  1.00         0.00     0.00      3.00    -1.00
+TOPOLOGY                                   N-in        ?      N-in
+    LOOP      1      6      6              2.00  ( -0.09)
+TRANSMEM      7     27     21 1.00 1.51
+    LOOP     28     38     11              1.00  (  0.21)
+TRANSMEM     39     59     21 1.00 1.52
+    LOOP     60     72     13              2.00  ( -0.00)
+TRANSMEM     73     93     21 1.00 1.87
+    LOOP     94     99      6              1.00  (  0.09)
+TRANSMEM    100    120     21 1.00 1.71
+    LOOP    121    124      4              3.00  ( -0.44)
+TRANSMEM    125    145     21 1.00 2.24
+    LOOP    146    153      8              0.00  (  0.90)
+TRANSMEM    154    174     21 1.00 1.89
+    LOOP    175    183      9              1.00  (  0.47)
+TRANSMEM    184    204     21 1.00 2.03
+    LOOP    205    214     10              1.00  (  1.02)
+TRANSMEM    215    235     21 1.00 2.13
+    LOOP    236    255     20              2.00  ( -0.10)
+TRANSMEM    256    276     21 1.00 1.83
+    LOOP    277    307     31              8.00  ( -0.46)
+//
+
+Sequence : gi_16131578_ref_NP_418166_1_  (391 res)
+MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAFSVYLAGMAAAMLFAGK
+VADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQGLGAGCCYVVAFAILRDTL
+DDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLFWAMAMMGIAVLMLSLFILKE
+TRPAAPAASDKPRENSESLLNRFFLSRVVITTLSVSVILTFVNTSPVLLMEIMGFERGEY
+ATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQVLFLAAGITLAVSPSHAVSLFGITL
+ICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIAQVCGSSLWIWLAAVVGIGAWNMLIGI
+LIACSIVSLLLIMFVAPGRPVAAHEEIHHHA
+
+Found: 12 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1     4 - 24    1.353 Certain
+     2    39 - 59    1.825 Certain
+     3    69 - 89    1.973 Certain
+     4    97 - 117   1.153 Certain
+     5   132 - 152   1.634 Certain
+     6   158 - 178   2.519 Certain
+     7   208 - 228   1.568 Certain
+     8   241 - 261   1.864 Certain
+     9   269 - 289   1.729 Certain
+    10   293 - 313   2.136 Certain
+    11   316 - 336   0.657 Putative
+    12   356 - 376   2.433 Certain
+
+Total of 2 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  0.14        16.00     0.00      3.00    -1.00
+TOPOLOGY                                   N-in        ?      N-in
+CYT_LOOP      1      3      3              1.00  ( -0.06)
+TRANSMEM      4     24     21 1.00 1.35
+EXT_LOOP     25     38     14              1.00  ( -0.52)
+TRANSMEM     39     59     21 1.00 1.83
+CYT_LOOP     60     68      9              4.00  (  0.04)
+TRANSMEM     69     89     21 1.00 1.97
+EXT_LOOP     90     96      7              1.00  ( -0.17)
+TRANSMEM     97    117     21 1.00 1.15
+CYT_LOOP    118    131     14              5.00  ( -1.04)
+TRANSMEM    132    152     21 1.00 1.63
+EXT_LOOP    153    157      5              1.00  (  0.47)
+TRANSMEM    158    178     21 1.00 2.52
+CYT_LOOP    179    207     29              6.00  ( -0.24)
+TRANSMEM    208    228     21 1.00 1.57
+EXT_LOOP    229    240     12              1.00  ( -0.37)
+TRANSMEM    241    261     21 1.00 1.86
+CYT_LOOP    262    268      7              2.00  ( -0.09)
+TRANSMEM    269    289     21 1.00 1.73
+EXT_LOOP    290    292      3              0.00  (  0.56)
+TRANSMEM    293    313     21 1.00 2.14
+CYT_LOOP    314    315      2              0.00  (  0.45)
+TRANSMEM    316    336     21 0.14 0.66
+EXT_LOOP    337    355     19              0.00  (  0.59)
+TRANSMEM    356    376     21 1.00 2.43
+CYT_LOOP    377    391     15              1.00  (  0.28)
+//
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   2                  1.00        15.00     0.00      3.00    -1.00
+TOPOLOGY                                   N-in        ?      N-in
+CYT_LOOP      1      3      3              1.00  ( -0.06)
+TRANSMEM      4     24     21 1.00 1.35
+EXT_LOOP     25     38     14              1.00  ( -0.52)
+TRANSMEM     39     59     21 1.00 1.83
+CYT_LOOP     60     68      9              4.00  (  0.04)
+TRANSMEM     69     89     21 1.00 1.97
+EXT_LOOP     90     96      7              1.00  ( -0.17)
+TRANSMEM     97    117     21 1.00 1.15
+CYT_LOOP    118    131     14              5.00  ( -1.04)
+TRANSMEM    132    152     21 1.00 1.63
+EXT_LOOP    153    157      5              1.00  (  0.47)
+TRANSMEM    158    178     21 1.00 2.52
+CYT_LOOP    179    207     29              6.00  ( -0.24)
+TRANSMEM    208    228     21 1.00 1.57
+EXT_LOOP    229    240     12              1.00  ( -0.37)
+TRANSMEM    241    261     21 1.00 1.86
+CYT_LOOP    262    268      7              2.00  ( -0.09)
+TRANSMEM    269    289     21 1.00 1.73
+EXT_LOOP    290    292      3              0.00  (  0.56)
+TRANSMEM    293    313     21 1.00 2.14
+CYT_LOOP    314    355     42              1.00  (  0.91)
+TRANSMEM    356    376     21 1.00 2.43
+EXT_LOOP    377    391     15              1.00  (  0.28)
+//
+
+Sequence : gi_16131585_ref_NP_418173_1_  (195 res)
+MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSGPFYCVDEVEHLCPWCI
+ADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDEMLKELVERTPGYHGWQQE
+FWLAHCGDFCVFIGYVGWNDIKDRLDEFANLEEDCENFGIRNSDLAKCLQKGGHCQGYLF
+RCLHCGKLRLWGDFS
+
+Found: 1 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1   119 - 139   0.751 Putative
+
+Total of 2 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  0.38        -8.00    -0.21      3.00     0.52
+TOPOLOGY                                  N-out     N-in      N-in
+EXT_LOOP      1    118    118           (  8.00)   -0.21
+TRANSMEM    119    139     21 0.38 0.75
+CYT_LOOP    140    195     56              8.00  ( -1.38)
+//
+
+Sequence : gi_16131606_ref_NP_418194_1_  (271 res)
+MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINIDSMFFSVVLGLLFLVLF
+RSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIAPLALTIFVWVFLMNLMDL
+LPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFILILFYSIKMKGIGGFTKELTL
+QPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAGELIFILIAGLLPWWSQWILNVP
+WAIFHILIITLQAFIFMVLTIVYLSMASEEH
+
+Found: 6 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1    43 - 63    1.899 Certain
+     2    98 - 118   1.967 Certain
+     3   147 - 167   2.078 Certain
+     4   183 - 203   0.695 Putative
+     5   217 - 237   1.604 Certain
+     6   243 - 263   2.143 Certain
+
+Total of 2 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  1.00        -9.00     0.00     -6.00     0.60
+TOPOLOGY                                  N-out        ?     N-out
+EXT_LOOP      1     42     42              1.00  (  0.34)
+TRANSMEM     43     63     21 1.00 1.90
+CYT_LOOP     64     97     34              7.00  (  1.62)
+TRANSMEM     98    118     21 1.00 1.97
+EXT_LOOP    119    146     28              1.00  (  0.31)
+TRANSMEM    147    167     21 1.00 2.08
+CYT_LOOP    168    216     49              5.00  (  0.94)
+TRANSMEM    217    237     21 1.00 1.60
+EXT_LOOP    238    242      5              0.00  (  1.11)
+TRANSMEM    243    263     21 1.00 2.14
+CYT_LOOP    264    271      8              0.00  (  0.00)
+//
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   2                  0.24        -5.00     0.00     -6.00     0.60
+TOPOLOGY                                  N-out        ?     N-out
+EXT_LOOP      1     42     42              1.00  (  0.34)
+TRANSMEM     43     63     21 1.00 1.90
+CYT_LOOP     64     97     34              7.00  (  1.62)
+TRANSMEM     98    118     21 1.00 1.97
+EXT_LOOP    119    146     28              1.00  (  0.31)
+TRANSMEM    147    167     21 1.00 2.08
+CYT_LOOP    168    182     15              3.00  ( -0.25)
+TRANSMEM    183    203     21 0.24 0.69
+EXT_LOOP    204    216     13              2.00  (  1.02)
+TRANSMEM    217    237     21 1.00 1.60
+CYT_LOOP    238    242      5              0.00  (  1.11)
+TRANSMEM    243    263     21 1.00 2.14
+EXT_LOOP    264    271      8              0.00  (  0.00)
+//
+
+Sequence : gi_67005950_ref_NP_418228_2_  (109 res)
+MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEIADEYQGKLTVAKLN
+IDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFLDANLA
+
+Found: 0 segments
+
+
+Sequence : gi_16131722_ref_NP_418318_1_  (298 res)
+MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKGATPAANPAQAAKDAEF
+IITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQTDKLIADMQAKGFSMMDVP
+VGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELINAGGPGMGIRVKLINNYMSIA
+LNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTTSWPNKVLSGDLSPAFMIDLAHK
+DLGIALDVANQLHVPMPLGAASREVYSQARAAGRGRQDWSAILEQVRVSAGMTAKVKM
+
+Found: 3 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1     1 - 21    1.358 Certain
+     2   124 - 144   1.104 Certain
+     3   147 - 167   0.810 Putative
+
+Total of 2 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  0.53         1.00    -1.38      1.00     0.00
+TOPOLOGY                                   N-in     N-in      N-in
+TRANSMEM      1     21     21 1.00 1.36
+EXT_LOOP     22    123    102           (  8.00)    0.35
+TRANSMEM    124    144     21 1.00 1.10
+CYT_LOOP    145    146      2              1.00  ( -1.31)
+TRANSMEM    147    167     21 0.53 0.81
+EXT_LOOP    168    298    131           ( 13.00)    1.03
+//
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   2                  1.00         0.00     0.47      1.00     0.00
+TOPOLOGY                                   N-in    N-out      N-in
+TRANSMEM      1     21     21 1.00 1.36
+    LOOP     22    123    102           (  8.00)    0.35
+TRANSMEM    124    144     21 1.00 1.10
+    LOOP    145    298    154           ( 14.00)    0.82
+//
+
+Sequence : gi_16131732_ref_NP_418328_1_  (211 res)
+MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLMQIMGTPQLARILHPFV
+GVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVGDTGRYNFGQKCVFWAAII
+FLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVALIVVIMVHIYAALWVKGTITA
+MVEGWVTSAWAKKHHPRWYREVRKTTEKKAE
+
+Found: 5 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1    20 - 40    2.242 Certain
+     2    55 - 75    2.115 Certain
+     3   113 - 133   2.476 Certain
+     4   148 - 168   2.039 Certain
+     5   171 - 191   0.691 Putative
+
+Total of 2 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  1.00        18.00     0.00     -1.00    -0.43
+TOPOLOGY                                   N-in        ?     N-out
+CYT_LOOP      1     19     19              5.00  ( -0.36)
+TRANSMEM     20     40     21 1.00 2.24
+EXT_LOOP     41     54     14              1.00  ( -0.20)
+TRANSMEM     55     75     21 1.00 2.11
+CYT_LOOP     76    112     37              7.00  (  0.42)
+TRANSMEM    113    133     21 1.00 2.48
+EXT_LOOP    134    147     14              2.00  (  0.83)
+TRANSMEM    148    168     21 1.00 2.04
+CYT_LOOP    169    211     43              9.00  (  0.43)
+//
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   2                  0.23         2.00     0.00     -1.00    -0.43
+TOPOLOGY                                   N-in        ?     N-out
+CYT_LOOP      1     19     19              5.00  ( -0.36)
+TRANSMEM     20     40     21 1.00 2.24
+EXT_LOOP     41     54     14              1.00  ( -0.20)
+TRANSMEM     55     75     21 1.00 2.11
+CYT_LOOP     76    112     37              7.00  (  0.42)
+TRANSMEM    113    133     21 1.00 2.48
+EXT_LOOP    134    147     14              2.00  (  0.83)
+TRANSMEM    148    168     21 1.00 2.04
+CYT_LOOP    169    170      2              1.00  (  0.61)
+TRANSMEM    171    191     21 0.23 0.69
+EXT_LOOP    192    211     20              8.00  ( -0.13)
+//
+
+Sequence : gi_16131829_ref_NP_418427_1_  (196 res)
+MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGDGQIYRRILDLIWETLT
+VKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSELVHSRLSGETLEHAVEVS
+KTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEIFRLLAECEERDIELIKGLRA
+DLREAGESNIGIIFQQ
+
+Found: 0 segments
+
+
+Sequence : gi_16131888_ref_NP_418486_1_  (107 res)
+MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRTVTHQTLGDYIRQRRLL
+LAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSDYRHRL
+
+Found: 0 segments
+
+
+Sequence : gi_16131894_ref_NP_418492_1_  (104 res)
+MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLIAFAPGWLGTPLNPNTS
+VTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQAS
+
+Found: 2 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1    27 - 47    2.426 Certain
+     2    64 - 84    2.112 Certain
+
+Total of 1 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  1.00         7.00     0.00      2.00    -0.11
+TOPOLOGY                                   N-in        ?      N-in
+CYT_LOOP      1     26     26              5.00  ( -0.65)
+TRANSMEM     27     47     21 1.00 2.43
+EXT_LOOP     48     63     16              1.00  (  0.74)
+TRANSMEM     64     84     21 1.00 2.11
+CYT_LOOP     85    104     20              2.00  (  0.18)
+//
+
+Sequence : gi_16131928_ref_NP_418526_1_  (241 res)
+MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAARFEVNRHTLRRAIDQLV
+EKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPTSEKLLSVLRPASGHVADA
+LGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQRFDSGSLHDFLREQTGIALR
+RSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDGESSPAEYSVSLTRADMIEFTME
+H
+
+Found: 0 segments
+
+
+Sequence : gi_16132032_ref_NP_418631_1_  (324 res)
+MPVMISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLIALPIAWLGRVRLR
+QLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLPVVIPVFANLLYSQRD
+GKLAWGKLAPALICIGIGLACVNIAELNHGLPDFDWARYTSGIVLALVSVVCWAWYALRN
+ARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQTPDFSLPFGPRPLVFISLM
+VAIAVLCSWVGALCWNVASQLLPTVILGPLIVFETLAGLLYTFLLRQQMPPLMTLSGIAL
+LVIGVVIAVRAKPEKPLTESVSES
+
+Found: 10 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1     8 - 28    2.168 Certain
+     2    37 - 57    1.015 Certain
+     3    67 - 87    1.662 Certain
+     4    92 - 112   1.876 Certain
+     5   125 - 145   1.478 Certain
+     6   159 - 179   1.771 Certain
+     7   198 - 218   1.609 Certain
+     8   233 - 253   2.235 Certain
+     9   261 - 281   1.449 Certain
+    10   289 - 309   2.132 Certain
+
+Total of 1 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  1.00        11.00     0.00      0.00     0.00
+TOPOLOGY                                   N-in        ?         ?
+CYT_LOOP      1      7      7              0.00  (  0.70)
+TRANSMEM      8     28     21 1.00 2.17
+EXT_LOOP     29     36      8              1.00  (  0.27)
+TRANSMEM     37     57     21 1.00 1.01
+CYT_LOOP     58     66      9              5.00  ( -1.44)
+TRANSMEM     67     87     21 1.00 1.66
+EXT_LOOP     88     91      4              1.00  ( -0.76)
+TRANSMEM     92    112     21 1.00 1.88
+CYT_LOOP    113    124     12              3.00  ( -0.16)
+TRANSMEM    125    145     21 1.00 1.48
+EXT_LOOP    146    158     13              1.00  ( -0.34)
+TRANSMEM    159    179     21 1.00 1.77
+CYT_LOOP    180    197     18              4.00  ( -0.11)
+TRANSMEM    198    218     21 1.00 1.61
+EXT_LOOP    219    232     14              1.00  (  0.30)
+TRANSMEM    233    253     21 1.00 2.24
+CYT_LOOP    254    260      7              0.00  (  0.46)
+TRANSMEM    261    281     21 1.00 1.45
+EXT_LOOP    282    288      7              1.00  ( -0.62)
+TRANSMEM    289    309     21 1.00 2.13
+CYT_LOOP    310    324     15              3.00  (  0.32)
+//
+
+Sequence : gi_90111709_ref_NP_418651_2_  (340 res)
+MPQSLPDTTTPKRRFRWPTGMPQLVALLLVLLVDSLVAPHFWQVVLQDGRLFGSPIDILN
+RAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVAGFSLPIVLLSALGTGILA
+GLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVTFNSPDLSWFGSGSLLFLPTP
+VIIAVLTLILFWLLTRKTALGMFIEAVGINIRAAKNAGVNTRIIVMLTYVLSGLCAAIAG
+IIVAADIRGADANNAGLWLELDAILAVVIGGGSLMGGRFNLLLSVVGALIIQGMNTGILL
+SGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGVRSRDKT
+
+Found: 8 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1    17 - 37    1.334 Certain
+     2    62 - 82    1.965 Certain
+     3    95 - 115   1.983 Certain
+     4   126 - 146   1.300 Certain
+     5   175 - 195   2.272 Certain
+     6   223 - 243   1.942 Certain
+     7   281 - 301   1.350 Certain
+     8   305 - 325   0.631 Putative
+
+Total of 2 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  0.08         6.00     0.00      4.00    -0.60
+TOPOLOGY                                   N-in        ?      N-in
+CYT_LOOP      1     16     16              4.00  ( -0.48)
+TRANSMEM     17     37     21 1.00 1.33
+EXT_LOOP     38     61     24              2.00  (  0.55)
+TRANSMEM     62     82     21 1.00 1.96
+CYT_LOOP     83     94     12              0.00  (  0.91)
+TRANSMEM     95    115     21 1.00 1.98
+EXT_LOOP    116    125     10              0.00  (  0.12)
+TRANSMEM    126    146     21 1.00 1.30
+CYT_LOOP    147    174     28              1.00  (  1.01)
+TRANSMEM    175    195     21 1.00 2.27
+EXT_LOOP    196    222     27              5.00  (  0.03)
+TRANSMEM    223    243     21 1.00 1.94
+CYT_LOOP    244    280     37              2.00  (  0.01)
+TRANSMEM    281    301     21 1.00 1.35
+EXT_LOOP    302    304      3              0.00  (  0.76)
+TRANSMEM    305    325     21 0.08 0.63
+CYT_LOOP    326    340     15              5.00  ( -0.22)
+//
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   2                  1.00        -5.00     0.00      4.00    -0.60
+TOPOLOGY                                  N-out        ?      N-in
+EXT_LOOP      1     16     16              4.00  ( -0.48)
+TRANSMEM     17     37     21 1.00 1.33
+CYT_LOOP     38     61     24              2.00  (  0.55)
+TRANSMEM     62     82     21 1.00 1.96
+EXT_LOOP     83     94     12              0.00  (  0.91)
+TRANSMEM     95    115     21 1.00 1.98
+CYT_LOOP    116    125     10              0.00  (  0.12)
+TRANSMEM    126    146     21 1.00 1.30
+EXT_LOOP    147    174     28              1.00  (  1.01)
+TRANSMEM    175    195     21 1.00 2.27
+CYT_LOOP    196    222     27              5.00  (  0.03)
+TRANSMEM    223    243     21 1.00 1.94
+EXT_LOOP    244    280     37              2.00  (  0.01)
+TRANSMEM    281    301     21 1.00 1.35
+CYT_LOOP    302    340     39              6.00  (  1.16)
+//
+
+Sequence : gi_16132087_ref_NP_418686_1_  (439 res)
+MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQAVLHSIQNGIGSTLGG
+LAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALVITGLVVGLAMFFEVGFVL
+LLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGPTAIATIFEANLGTTLLYGFI
+ITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEEMPSFWNSIFAAVIPVILMAIAA
+VCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFTLGRRNGRTIEQIMDIIGDSIGAIA
+MIVFIIAGGGAFKQVLVDSGVGHYISHLMTGTTLSPLLMCWTVAALLRIALGSATVAAIT
+TAGVVLPIINVTHADPALMVLATGAGSVIASHVNDPGFWLFKGYFNLTVGETLRTWTVME
+TLISIMGLLGVLAINAVLH
+
+Found: 11 segments
+
+Candidate membrane-spanning segments:
+
+ Helix Begin - End   Score Certainity
+     1     1 - 21    2.345 Certain
+     2    52 - 72    1.959 Certain
+     3   116 - 136   2.078 Certain
+     4   147 - 167   0.917 Putative
+     5   174 - 194   1.965 Certain
+     6   222 - 242   2.086 Certain
+     7   258 - 278   2.048 Certain
+     8   292 - 312   1.893 Certain
+     9   327 - 347   1.806 Certain
+    10   374 - 394   1.394 Certain
+    11   418 - 438   1.682 Certain
+
+Total of 2 structures are to be tested
+
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   1                  1.00         6.00     0.00     -1.00     0.00
+TOPOLOGY                                   N-in        ?     N-out
+TRANSMEM      1     21     21 1.00 2.34
+EXT_LOOP     22     51     30              1.00  (  1.22)
+TRANSMEM     52     72     21 1.00 1.96
+CYT_LOOP     73    115     43              5.00  (  0.12)
+TRANSMEM    116    136     21 1.00 2.08
+EXT_LOOP    137    173     37              0.00  (  1.05)
+TRANSMEM    174    194     21 1.00 1.96
+CYT_LOOP    195    221     27              3.00  ( -0.02)
+TRANSMEM    222    242     21 1.00 2.09
+EXT_LOOP    243    257     15              2.00  ( -0.24)
+TRANSMEM    258    278     21 1.00 2.05
+CYT_LOOP    279    291     13              3.00  ( -1.17)
+TRANSMEM    292    312     21 1.00 1.89
+EXT_LOOP    313    326     14              1.00  (  1.69)
+TRANSMEM    327    347     21 1.00 1.81
+CYT_LOOP    348    373     26              1.00  (  0.66)
+TRANSMEM    374    394     21 1.00 1.39
+EXT_LOOP    395    417     23              2.00  (  0.27)
+TRANSMEM    418    438     21 1.00 1.68
+CYT_LOOP    439    439      1              0.00  (  0.26)
+//
+HEADER    START   STOP    LEN PROB   HP  DARGLYS  DCYTEXT DNCHARGE DNNEGPOS
+TOPOLOGY   2                  0.79         2.00     0.00     -1.00     0.00
+TOPOLOGY                                   N-in        ?     N-out
+TRANSMEM      1     21     21 1.00 2.34
+EXT_LOOP     22     51     30              1.00  (  1.22)
+TRANSMEM     52     72     21 1.00 1.96
+CYT_LOOP     73    115     43              5.00  (  0.12)
+TRANSMEM    116    136     21 1.00 2.08
+EXT_LOOP    137    146     10              0.00  (  1.59)
+TRANSMEM    147    167     21 0.79 0.92
+CYT_LOOP    168    173      6              0.00  ( -0.43)
+TRANSMEM    174    194     21 1.00 1.96
+EXT_LOOP    195    221     27              3.00  ( -0.02)
+TRANSMEM    222    242     21 1.00 2.09
+CYT_LOOP    243    257     15              2.00  ( -0.24)
+TRANSMEM    258    278     21 1.00 2.05
+EXT_LOOP    279    291     13              3.00  ( -1.17)
+TRANSMEM    292    312     21 1.00 1.89
+CYT_LOOP    313    326     14              1.00  (  1.69)
+TRANSMEM    327    347     21 1.00 1.81
+EXT_LOOP    348    373     26              1.00  (  0.66)
+TRANSMEM    374    394     21 1.00 1.39
+CYT_LOOP    395    417     23              2.00  (  0.27)
+TRANSMEM    418    438     21 1.00 1.68
+EXT_LOOP    439    439      1              0.00  (  0.26)
+//